CN104350836A - New application of melatonin to improving saline-alkaline stress resistance of plant seeds - Google Patents
New application of melatonin to improving saline-alkaline stress resistance of plant seeds Download PDFInfo
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- CN104350836A CN104350836A CN201410629751.XA CN201410629751A CN104350836A CN 104350836 A CN104350836 A CN 104350836A CN 201410629751 A CN201410629751 A CN 201410629751A CN 104350836 A CN104350836 A CN 104350836A
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Abstract
The invention relates to new application of melatonin to improving saline-alkaline stress resistance of plant seeds. A product taking the melatonin as an effective ingredient is applied to the plant seeds under saline-alkaline stress, and the melatonin is a compound with the high lipophicity and hydrophilcity and can get in and out of plant cells freely, so that the melatonin can play a role in plant tissues. The melatonin can be applied to the plant seeds under the saline-alkaline stress conveniently, can be used for promoting the germination rate and the germination potential and reducing the salt damage rate of the plant seeds under the saline-alkaline stress, and can be used for promoting the seed germination and seed vigor, so that the purposes of keeping a full stand of seedlings, strengthening the seedlings, and preventing the reduction of yield and quality, caused by the saline-alkaline stress, can be achieved.
Description
Technical field
the present invention relates to the new opplication of a kind of epiphysin in raising plant seed Salt And Alkali Tolerance is coerced.
Background technology
epiphysin (MLT), as a kind of physiological regulation agent of wide spectrum, is present in most biologic artifact.Research shows, epiphysin can improve plant resistance (arid, high temperature, damage to plants caused by sudden drop in temperature, fungal disease etc.), all have certain regulating action to plant circadian rhythm.Epiphysin, originally as Inner source metabolite, can be biodegradable, and have certain effect to body-care, the safe noresidue of avirulence, can be used for land for growing field crops and conventional agriculture production.
Research shows, China's saline land area increases just year by year, and salinization and alkalization increases the weight of gradually, causes the deterioration of the ecological environment, directly threatens the socioeconomic development such as agricultural, animal husbandry.Saline-alkali soil is otherwise known as " green forbidden area ", without any value.And coarse cereals are high as a kind of rich in nutrition content, content, be acknowledged as food source human health to important function, most economical, the most effective and safest grain, in addition coarse cereals possess the features such as anti-saline and alkaline, impoverishment tolerant, strong stress resistance, have the potentiality well adapting to normal growth on saline land.On this basis, can improve with the use of some the bioactivator that seed vitality, germination rate etc. were coerced, improved to plant seed Salt And Alkali Tolerance, then farthest can ensure the normal growth of crop in saline land, thus reach the object increasing economic benefit.Simultaneously for effectively alleviating Chinese people ground contradiction, increase grain yield, improve and be of great significance with the ecotope tool of salinization of soil region barrenly, producing promotion developing agricultural on saline land has major and immediate significance and far-reaching influence.
It is difficult that saline Land can make plant absorb water, and wilts lethal, affects plant nutrition and absorb.Li etc. are at Journal of Pineal Research magazine the 53rd volume the 3rd phase The mitigation effects of exogenous melatonin on salinity-induced stress in
malus hupehensisone literary composition sodium chloride is as salt environment, additional epiphysin is applied in the environment of Malus spectabilis growth, points out that external source epiphysin significantly can alleviate the impact of hypersaline environment on plant growth, maintains the photosynthetic efficiency that plant is higher, repair the oxidative damage that high salt brings, activate relevant enzymatic synthesis gene.Arnao uses the root of sodium chloride, zinc sulphate, hydrogen peroxide treatment barley respectively in Journal of Pineal Research magazine the 46th volume the 3rd phase Chemical stress by different agents affects the melatonin content of barley roots mono-literary composition, cultivate through 72 h, analyze by methods such as liquid chromatogram, mass spectral analysis, fluoroscopic examinations, discovery is used external source epiphysin and is absorbed through Barley Roots, and endogenous melatonin content can be induced to increase.These all illustrate that epiphysin can alleviate the coercion of salt ion to plant.But the applicable cases of epiphysin under the saline and alkaline common stressful environmental of plant seed, also rarely has report.
Summary of the invention
For the deficiency of above technical problem, the object of the present invention is to provide the new opplication that a kind of epiphysin is being alleviated plant and suffered in Saline Alkali Stress.
To achieve these goals, present invention employs following technical scheme:
Take epiphysin as the application of product in raising plant seed Salt And Alkali Tolerance is coerced of active ingredient.
Concrete, described application method is: the product taking epiphysin as active ingredient is applied to the plant seed by Saline Alkali Stress, preferred mung bean seed.
Further, described application method is specially:
1) with 70% ethanol, carried out to plant seed surface sterilization 3-5 second;
2) repeatedly plant seed is rinsed 3-4 time with sterile water again;
3) product being active ingredient in order to epiphysin by the plant seed handled well under Saline Alkali Stress soaks 12 h;
4) cultivate under the plant seed of immersion 12 h being placed in 25 DEG C of conditions;
5) product being active ingredient in order to epiphysin is administered to 4) described in plant seed in, frequency of administration is every day 1 time; Total application times is 5 times.
More specifically, described amount of application is: the amount of application of every 10 plant seeds is 1 mL.
More specifically, the described aqueous solution taking epiphysin as the product of active ingredient and specifically can be epiphysin, also can with agriculturally acceptable carrier or solvent are processed into formulation known at present in a known way.
The structural formula of this epiphysin is as shown in Equation 1:
Formula 1
More specifically, the concentration of the aqueous solution of described epiphysin is 0.01-10 μm of ol/L, and preferred concentration is 0.1-1 μm of ol/L.
More specifically, described Saline Alkali Stress environment is: NaCl:Na
2sO
4: NaHCO
3: Na
2cO
3mol ratio is the complex saline-alkali solution of 3:3:5:1, and concentration is 100 m mol/L, pH=8.9-9.2.
The Salt And Alkali Tolerance of described raising plant seed coerce into following any one:
1) germination rate of plant seed is improved;
2) germination vigor of plant seed is significantly improved;
3) germination index of plant seed is improved;
4) vitality index of plant seed is improved;
5) the salt damage rate suffered by plant seed is reduced;
The germination rate of described plant seed is the germination rate of the 5th day, and described germination vigor is the seed germination vigor of the 2nd day.
More specifically, described epiphysin can add surfactant and is applied to plant.Can use separately, also can mix with chemical substances such as fertilizer, trace element, insecticide, bactericide, amino acid, other plant growth regulators and use.
The present invention, relative to prior art, has following beneficial effect:
Because epiphysin is highly lipophilic and hydrophilic compound, plant cell of can freely coming in and going out, therefore can play function in plant tissue.Epiphysin of the present invention is applied to the plant seed by Saline Alkali Stress, not only use conveniently, and the germination rate of plant seed under Saline Alkali Stress, germination vigor, reduction salt damage rate can be promoted, promote seed germination and seed vitality, thus reach keep a full stand of seedings, strong sprout, reduce the object that the yield and quality that causes of Saline Alkali Stress declines.
Embodiment
Below in conjunction with specific embodiment, the present invention is further elaborated, but the present invention is not limited to following examples.Described method is conventional method if no special instructions.Described raw material all can obtain from open commercial sources if no special instructions.
Embodiment 1: epiphysin is alleviating the application in mung bean seed Saline Alkali Stress
Select the mung bean seed 360 of full health, be specially the mung bean seed that 100-grain weight is 6.23 g.First with 70% ethanol, carried out to seed surface sterilization 3-5 second, then repeatedly seed is rinsed 3-4 time with sterile water, be 100 m mol/L with salt ionic concentration respectively by the seed handled well, the saline and alkaline mixed liquor (S+A) of pH=9, saline and alkaline mixed liquor add 6 kinds of solution such as variable concentrations (4 concentration) the epiphysin aqueous solution (S+A+MT) and sterile water etc. and soak 12 h, with sterile water immersion for contrast;
Three groups of parallel laboratory tests are done in each process.
The seed of immersion 12 h is put in the 9 cm glass culture dishs being covered with the filter paper soaked with sterile water in advance, cultivates under being placed in 25 DEG C of conditions; The product being active ingredient in order to epiphysin is applied in cultivated plant seed, and frequency of administration is every day 1 time; The amount of application of every 10 plant seeds is 1 mL, and total application times is 5 times.
Every day is added up and records each process seed sprouting situation, calculates germination vigor, calculate final germination rate, and calculate germination index when the 5th day when the 2nd day, weighs radicle and weigh and calculate vitality index when the 5th day;
Seed number/total seed number of germination rate=germination; Seed number is planted experimentally in normal germination grain number/confession in germination vigor=germination vigor number of days; Germination index=∑ (Gt/Dt), Gt is t time potentiality of seed, and Dt is corresponding germinating time; Vitality index=S × germination index, S is radicle fresh weight; Salt damage rate=(CK germination vigor-process germination vigor)/CK germination vigor × 100, CK is control group;
According to as above step, acquired results is all listed in table 1.
Table 1, epiphysin suffer the mitigation 1 of Saline Alkali Stress to mung bean seed
| Process | Germination rate (%) | Germination vigor (%) | Germination index | Vitality index | Salt damage rate (%) |
| CK | 100 | 96.3±0.6 | 19.8±0.3 | 49.9±0.4 | -- |
| S+A | 80 | 53.3±0.7 | 10.1±1.0 | 3.3±0.6 | 44.2±0.8 |
| S+A+0.01 μmol/L MT | 90 | 73.3±0.7 | 12.1±0.4 | 4.9±0.9 | 23.7±0.4 |
| S+A+0.1 μmol/L MT | 90 | 80.0±0.5 | 11.5±0.2 | 5.1±0.7 | 16.7±0.4 |
| S+A+1 μmol/L MT | 80 | 80.0±1.0 | 11.2±0.8 | 5.0±0.7 | 15.0±0.5 |
| S+A+10μmol/L MT | 90 | 80.0±0.8 | 11.1±1.0 | 5.4±1.0 | 17.1±0.5 |
In table, CK is sterile water process, and S+A is 100 m mol/L saline solutions, and MT is epiphysin;
As shown in Table 1, the epiphysin process of 4 kinds of variable concentrations all has obvious mitigation to the salt damage of mung bean seed, compared with control group CK, mung bean seed is under the condition having epiphysin, indices in seed germination all increases, and shows that epiphysin effectively can alleviate the negative effect because Saline Alkali Stress brings to seed.
Claims (10)
1. be the application of product in raising plant seed Salt And Alkali Tolerance is coerced of active ingredient with epiphysin.
2. application according to claim 1, is characterized in that: described application method is:
The product taking epiphysin as active ingredient is applied to the plant seed by Saline Alkali Stress.
3. application according to claim 2, is characterized in that: described application method is specially:
With 70% ethanol, carried out to plant seed surface sterilization 3-5 second;
Repeatedly plant seed is rinsed 3-4 time again with sterile water;
The product being active ingredient in order to epiphysin under Saline Alkali Stress by the plant seed handled well soaks 12 h;
Cultivate under the plant seed of immersion 12 h is placed in 25 DEG C of conditions;
The product being active ingredient in order to epiphysin is applied in step 4) described in plant seed in, frequency of administration is every day 1 time; Total application times is 5 times.
4. the application according to Claims 2 or 3, is characterized in that: described amount of application is: the amount of application of every 10 plant seeds is 1 mL; Described Saline Alkali Stress environment is: NaCl:Na
2sO
4: NaHCO
3: Na
2cO
3mol ratio is the complex saline-alkali solution of 3:3:5:1, and concentration is 100 m mol/L, pH is 8.9-9.2.
5. the application according to Claims 2 or 3, is characterized in that: the described aqueous solution taking epiphysin as the product of active ingredient and comprise epiphysin.
6. application according to claim 5, is characterized in that: the concentration of the aqueous solution of described epiphysin is 0.01-10 μm of ol/L.
7. application according to claim 5, is characterized in that: the concentration of the aqueous solution of described epiphysin is 0.1-1 μm of ol/L.
8. the application according to Claims 2 or 3, is characterized in that: described plant seed is mung bean seed.
9. application according to claim 2, is characterized in that: described is that the method for application of the product of active ingredient comprises epiphysin and adds surfactant and be applied to plant with epiphysin; Use separately; Mix with one or several in fertilizer, trace element, insecticide, bactericide, plant growth regulator and use.
10. the application according to any one of claim 1-9, is characterized in that: the Salt And Alkali Tolerance of described raising plant seed coerce into following any one:
1) germination rate of plant seed is improved;
2) germination vigor of plant seed is significantly improved;
3) germination index of plant seed is improved;
4) vitality index of plant seed is improved;
5) the salt damage rate suffered by plant seed is reduced;
The germination rate of described plant seed is the germination rate of the 5th day, and described germination vigor is the seed germination vigor of the 2nd day.
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105830731A (en) * | 2016-04-26 | 2016-08-10 | 湖南农业大学 | Method for promoting growth of root systems of medicago sativa seedlings under stress of acid aluminum |
| CN106034463A (en) * | 2016-05-26 | 2016-10-26 | 中国农业大学 | Method for promoting germination of oat aged seeds and growth of seedlings |
| CN106068779A (en) * | 2016-06-21 | 2016-11-09 | 中国农业大学 | A kind of method utilizing melatonin to strengthen the high temperature resistant storage of Herba bromi japonici Priming Seeds |
| CN106069693A (en) * | 2016-07-05 | 2016-11-09 | 中国农业科学院油料作物研究所 | A kind of compound adjusting control agent is in the application alleviated during Brassica campestris L salt damage is coerced |
| CN107347605A (en) * | 2017-08-03 | 2017-11-17 | 中国农业科学院油料作物研究所 | A kind of application of epiphysin in peanut salt damage stress is alleviated |
| CN107409532A (en) * | 2017-07-21 | 2017-12-01 | 广西柳城县成霖农业科技有限公司 | A kind of seed-soaking method for improving Soybean Resistance Saline Alkali Stress ability |
| CN108719299A (en) * | 2018-06-28 | 2018-11-02 | 陕西中医药大学 | A kind of promotion astragalus membranaceus seed germinant and preparation method thereof |
| CN110150119A (en) * | 2019-05-31 | 2019-08-23 | 扬州大学 | Isothiocyanate content and the production method of yield is improved in a kind of enrichment Brassica genus shoot vegetable |
| CN110367053A (en) * | 2019-08-13 | 2019-10-25 | 中国农业科学院农业资源与农业区划研究所 | A method of Leymus chinensis seeds vigor is improved by spermidine |
| CN110679410A (en) * | 2019-11-13 | 2020-01-14 | 上海市农业科学院 | Treatment method for improving heat resistance of lettuce |
| CN111279836A (en) * | 2020-04-08 | 2020-06-16 | 贵州师范大学 | Method for improving activity of sweet corn seeds under salt stress |
| CN112602713A (en) * | 2020-12-17 | 2021-04-06 | 青岛农业大学 | Alkali stress treating agent and preparation method and application thereof |
| CN114342599A (en) * | 2022-01-24 | 2022-04-15 | 黑龙江八一农垦大学 | Method for breaking cranberry seed dormancy by exogenous hormone |
| CN115152771A (en) * | 2022-05-13 | 2022-10-11 | 中国科学院东北地理与农业生态研究所 | Application of melatonin in cultivation of suaeda glauca |
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Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105830731A (en) * | 2016-04-26 | 2016-08-10 | 湖南农业大学 | Method for promoting growth of root systems of medicago sativa seedlings under stress of acid aluminum |
| CN106034463B (en) * | 2016-05-26 | 2018-11-27 | 中国农业大学 | A method of promoting the sprouting of oat Aged Seeds and growth of seedling using epiphysin |
| CN106034463A (en) * | 2016-05-26 | 2016-10-26 | 中国农业大学 | Method for promoting germination of oat aged seeds and growth of seedlings |
| CN106068779A (en) * | 2016-06-21 | 2016-11-09 | 中国农业大学 | A kind of method utilizing melatonin to strengthen the high temperature resistant storage of Herba bromi japonici Priming Seeds |
| CN106068779B (en) * | 2016-06-21 | 2019-06-04 | 中国农业大学 | A method of causing seed high temperature resistant using epiphysin enhancing oat and stores |
| CN106069693A (en) * | 2016-07-05 | 2016-11-09 | 中国农业科学院油料作物研究所 | A kind of compound adjusting control agent is in the application alleviated during Brassica campestris L salt damage is coerced |
| CN107409532A (en) * | 2017-07-21 | 2017-12-01 | 广西柳城县成霖农业科技有限公司 | A kind of seed-soaking method for improving Soybean Resistance Saline Alkali Stress ability |
| CN107347605A (en) * | 2017-08-03 | 2017-11-17 | 中国农业科学院油料作物研究所 | A kind of application of epiphysin in peanut salt damage stress is alleviated |
| CN108719299A (en) * | 2018-06-28 | 2018-11-02 | 陕西中医药大学 | A kind of promotion astragalus membranaceus seed germinant and preparation method thereof |
| CN110150119A (en) * | 2019-05-31 | 2019-08-23 | 扬州大学 | Isothiocyanate content and the production method of yield is improved in a kind of enrichment Brassica genus shoot vegetable |
| CN110367053A (en) * | 2019-08-13 | 2019-10-25 | 中国农业科学院农业资源与农业区划研究所 | A method of Leymus chinensis seeds vigor is improved by spermidine |
| CN110367053B (en) * | 2019-08-13 | 2022-02-25 | 中国农业科学院农业资源与农业区划研究所 | Method for improving seed vigor of leymus chinensis through spermidine |
| CN110679410A (en) * | 2019-11-13 | 2020-01-14 | 上海市农业科学院 | Treatment method for improving heat resistance of lettuce |
| CN111279836A (en) * | 2020-04-08 | 2020-06-16 | 贵州师范大学 | Method for improving activity of sweet corn seeds under salt stress |
| CN112602713A (en) * | 2020-12-17 | 2021-04-06 | 青岛农业大学 | Alkali stress treating agent and preparation method and application thereof |
| CN114342599A (en) * | 2022-01-24 | 2022-04-15 | 黑龙江八一农垦大学 | Method for breaking cranberry seed dormancy by exogenous hormone |
| CN115152771A (en) * | 2022-05-13 | 2022-10-11 | 中国科学院东北地理与农业生态研究所 | Application of melatonin in cultivation of suaeda glauca |
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