CN107493726A - Application of the citrulling in the resistance to salt stress of vegetable seeds is improved - Google Patents
Application of the citrulling in the resistance to salt stress of vegetable seeds is improved Download PDFInfo
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- CN107493726A CN107493726A CN201710797746.3A CN201710797746A CN107493726A CN 107493726 A CN107493726 A CN 107493726A CN 201710797746 A CN201710797746 A CN 201710797746A CN 107493726 A CN107493726 A CN 107493726A
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- vegetable seeds
- salt stress
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- citrulling
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
Abstract
The present invention provides the application with citrulling in the resistance to salt stress of vegetable seeds is improved, by the citrulline administration in the vegetable seeds by salt stress, germination percentage, germinating energy, germination index, vitality index, root activity, radicle length and the hypocotyl length in Plants under Salt Stress can be improved, seed is promoted to sprout, so as to reach vegetable seeds survival rate height, the high effect of yield and quality.
Description
Technical field
The present invention relates to application of the citrulling in the resistance to salt stress of vegetable seeds is improved.
Background technology
In recent years, soil salinization problem becomes to become increasingly conspicuous, and the threat to agricultural is a global problem, with
The expansion of the modernization of industry, irrigated land and vinyl house area, soil secondary salinization getting worse, therefore salt tolerance of crop
Raising is one of great research of agricultural, and salt stress is to suppress one of plant growth, the important environmental factor for reducing crop yield.
Watermelon is one of China staple crops, and critical role is occupied in world's horticulture, and salt stress injures to caused by plant is
It is many, include the destruction etc. of osmotic stress, Ion toxicity and intracellular ion balance, be suppressed plant growth, light
Close and decline, energy consumption increase, aging accelerates, and finally makes plant dead.Salt stress turns into a great problem for influenceing watermelon facility cultivation,
The growth and development of watermelon seed are seriously affected, very big influence is caused to yield and quality.Therefore seed selection salt tolerance is strong
Variety of watermelon is significant for development watermelon facility cultivation.
The content of the invention
For mirror with this, the present invention proposes a kind of application of citrulling in the resistance to salt stress of vegetable seeds is improved.
The technical proposal of the invention is realized in this way：Application of the citrulling in the resistance to salt stress of vegetable seeds is improved.
Preferably, the application process is in the vegetable seeds by salt stress by citrulline administration.
Preferably, the application process is in the watermelon seed by salt stress by citrulline administration.
Preferably, the application concentration of the citrulling is 0.1~0.8mmol/L.
Preferably, the citrulline administration amount is that every 50 vegetable seeds amounts of application are 1~3mL；The salt stress environment
It is 130~140mmol/L for NaCl concentration.
Preferably, the application process comprises the following steps：
(1) vegetable seeds of mature and plump is chosen, first with sterilization 10~15min of liquid disinfectant, then with aseptic water washing plant
Seed；
(2) vegetable seeds for handling step (1) well soaks 4~6h under salt stress with the citrulling solution, then will
Vegetable seeds is placed in the culture dish for being covered with filter paper, and culture dish is placed in 28~32 DEG C of insulating box and cultivated；
(3) the citrulling solution is applied in culture dish, changed once every 22~28h, repeated 3~5 times.
Preferably, the thimerosal is the liquor potassic permanganate that mass concentration is 0.25~0.35%.
Compared with prior art, the beneficial effects of the invention are as follows：
The citrulline administration can be reduced into plant in the vegetable seeds by salt stress, the citrulling processing of suitable concentration
Absorption of the seed to sodium ion, citrulling interacts with the macromolecular substances in vegetable seeds, by adjusting inorganic ions
Ratio reduces the absorption to salt ion so as to maintaining intracellular infiltration, so as to improve the germination percentage of Plants under Salt Stress,
Germinating energy, germination index, vitality index, root activity, radicle length and hypocotyl length, promote seed to sprout, so as to reach plant
Seed viability is high, the high effect of yield and quality.
Embodiment
In order to be best understood from the technology of the present invention content, specific embodiment is provided below, the present invention is described further.
Embodiment 1
Application of the citrulling in the resistance to salt stress of vegetable seeds is improved, the application process is in by salt by citrulline administration
The watermelon seed of stress, the application concentration of the citrulling is 0.1mmol/L, and the citrulline administration amount is every 50 plant species
Sub- amount of application is 1mL；The salt stress environment is that NaCl concentration is 130mmol/L.
The application process comprises the following steps：
(1) vegetable seeds of mature and plump is chosen, is first 0.25% disinfecting solution of potassium permanganate 10min with mass concentration,
Aseptic water washing vegetable seeds is used again.
(2) vegetable seeds for handling step (1) well soaks 4h under salt stress with the citrulling solution, then by plant
Seed is placed in the culture dish for being covered with filter paper, and culture dish is placed in 28 DEG C of insulating box and cultivated.
(3) the citrulling solution is applied in culture dish, changes once, be repeated 3 times every 22h.
Embodiment 2
Application of the citrulling in the resistance to salt stress of vegetable seeds is improved, the application process is in by salt by citrulline administration
The watermelon seed of stress, the application concentration of the citrulling is 0.8mmol/L, and the citrulline administration amount is every 50 plant species
Sub- amount of application is 3mL；The salt stress environment is that NaCl concentration is 140mmol/L.
The application process comprises the following steps：
(1) vegetable seeds of mature and plump is chosen, is first 0.35% disinfecting solution of potassium permanganate 15min with mass concentration,
Aseptic water washing vegetable seeds is used again.
(2) vegetable seeds for handling step (1) well soaks 6h under salt stress with the citrulling solution, then by plant
Seed is placed in the culture dish for being covered with filter paper, and culture dish is placed in 32 DEG C of insulating box and cultivated.
(3) the citrulling solution is applied in culture dish, changes once, be repeated 5 times every 28h.
Embodiment 3
Application of the citrulling in the resistance to salt stress of vegetable seeds is improved, the application process is in by salt by citrulline administration
The watermelon seed of stress, the application concentration of the citrulling is 0.4mmol/L, and the citrulline administration amount is every 50 plant species
Sub- amount of application is 2mL；The salt stress environment is that NaCl concentration is 136.2mmol/L.
The application process comprises the following steps：
(1) vegetable seeds of mature and plump is chosen, is first 0.3% disinfecting solution of potassium permanganate 12min with mass concentration, then
With aseptic water washing vegetable seeds.
(2) vegetable seeds for handling step (1) well soaks 5h under salt stress with the citrulling solution, then by plant
Seed is placed in the culture dish for being covered with filter paper, and culture dish is placed in 30 DEG C of insulating box and cultivated.
(3) the citrulling solution is applied in culture dish, changes once, be repeated 4 times every 24h.
Wherein, the application process is in the vegetable seeds by salt stress, the administration of the citrulling by citrulline administration
Method also adds surfactant to be applied to plant including citrulling, to be administered alone, or with fertilizer, trace element, bactericide, kill
One or more of mixing in worm agent or plant growth regulator are applied.
Embodiment 4
First, seed salt tolerant optium concentration selects
1.1 choose the watermelon seed of mature and plump, are first 0.3% disinfecting solution of potassium permanganate 12min with mass concentration, then
With aseptic water washing 4 times, then with various concentration processing solutions (NaCl concentration is respectively 0,100,110,120,130,140,
150mmol/L) soaked seed 5h, and seed is placed in and is covered with the culture dish of 2 layers of filter paper, 50 seeds is each put, adds 2mL's
Treatment fluid, each processing are repeated 4 times, and are placed in light culture in 30 DEG C of constant incubator, and corresponding place is during which changed once per 24h
Manage liquid, culture dish random alignment.
1.2 index determining
Start the 2d after vernalization to start to record germinative number daily, record 8d, 2mm is exposed using radicle and sprouts shape as seed
State.Germination percentage is calculated, relative germination rate is calculated with the germination percentage of control group, according to the relation between relative germination rate and salinity
Regression equation is calculated, calculates the salt tolerant Appropriate that seed is sprouted, critical value and lethal value.Respectively handle as shown in table 1 below：
| NaCl concentration mmol/L | Relative germination rate % |
| 100 | 85.1 |
| 110 | 74.5 |
| 120 | 72.3 |
| 130 | 57.4 |
| 140 | 53.2 |
| 150 | 27.2 |
As seen from the above table, using concentration as transverse axis, relative germination rate is the longitudinal axis, it follows that equation of linear regression Y=-
1.0451X+192.34 R2=0.92324.The critical value of salt tolerant is：Work as Y=50%, X=136.2mmol/L, so salt stress
NaCl concentration be 136.2mmol/L.
2nd, application of the various concentrations citrulling in watermelon seed rudiment salt damage is improved
The watermelon seed of mature and plump is chosen, is first 0.3% disinfecting solution of potassium permanganate 12min with mass concentration, then use
Aseptic water washing 4 times, then respectively with five kinds of various concentrations citrulling solution, NaCl concentration of salt solution be 136.2mmol/L and
8 kinds of solution such as sterilized water (CK) are soaked seed 5h respectively, and seed is placed in and is covered with the culture dish of 2 layers of filter paper, each puts 50
Seed, 2mL citrulling solution is added, each processing is repeated 4 times, and is placed in light culture in 30 DEG C of constant incubator, during which every
24h changes once corresponding citrulling solution, culture dish random alignment, and each processing makees three groups of panel datas, chooses average
According to.
Germination percentage, germinating energy, germination index and the vitality index of seed are counted, and using TTC methods measure root activity.
Wherein：Subnumber × 100% is planted experimentally in germination percentage=chitting piece number/confession；Chitting piece in germinating energy=stipulated time
Number/for planting experimentally subnumber × 100% (being defined by 5d germination percentage)；Germination index (GI)=∑ (Gt/Dt)；Vitality index (VI)=
∑ (Gt/Dt) × S=GI × S；
Gt refers to the germinative number of t days, and Dt refers to corresponding germination number of days, and S is that average radicle is grown.Using 5d germination percentage as germination
Gesture, repeat during vernalization 8d to take 10 seed testing radicle lengths at random.
1st, it is as shown in table 2 below to improving application result of the watermelon seed in resistance to salt stress according to above-mentioned steps, citrulling：
Table 2：Citrulling is to improving application of the watermelon seed in resistance to salt stress
As seen from the above table, the processing of various concentrations citrulling has obvious mitigation to the salt damage of watermelon seed, and right
Compared according to a group CK, in the presence of citrulling, its every growth indexes increases watermelon seed, citrulling matching watermelon seed
The physiological property of son, greatly suppresses absorption of the watermelon seed to salt ion during growth so that citrulling effectively carries
The growth and cultivation of high salinity underground watermelon seed.
Embodiment 5
Control group is set to test
Control group 1
Using γ-aminobutyric acid in the application of resistance to salt stress Chinese and Western melon seed, the application concentration of the γ-aminobutyric acid is
1.5mmol/L, applied using conventional method.
Control group 2
Using calcium nitrate in the application of resistance to salt stress Chinese and Western melon seed, the application process is same as Example 1.
Control group 3
Using glycine in the application of resistance to salt stress Chinese and Western melon seed, the application process is same as Example 1.
Control group 4
Using alanine in the application of resistance to salt stress Chinese and Western melon seed, the application process uses conventional method.
Germination percentage, germinating energy, germination index and the vigor of seed under Statistics Implementation example 1~3 and the salt stress of control group 1~4
Index, and it is as shown in table 3 below using TTC methods measure root activity, experimental result：
As seen from the above table, control group 1~4 is relative to NaCl solution, the germination percentage of watermelon seed, germinating energy, germination index,
The raising effect and unobvious of vitality index and root activity in salt stress.The present invention is by studying different material to watermelon seed
Son acts in salt stress environment, and research finds that growth of the citrulling to watermelon seed in salt stress has raising effect.
In summary, a kind of answering in the resistance to salt stress of vegetable seeds is improved with citrulling provided by the present invention is used
With.By the citrulline administration in the vegetable seeds by salt stress, it is possible to increase germination percentage, germination in Plants under Salt Stress
Gesture, germination index, vitality index, root activity, radicle length and hypocotyl length, promote seed to sprout, so as to reach vegetable seeds
Survival rate is high, the high effect of yield and quality.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
God any modification, equivalent substitution and improvements made etc., should be included in the scope of the protection with principle.
Claims (7)
1. application of the citrulling in the resistance to salt stress of vegetable seeds is improved.
2. application according to claim 1, it is characterised in that：The application process is in by salt stress by citrulline administration
Vegetable seeds.
3. application according to claim 2, it is characterised in that：The application process is in by salt stress by citrulline administration
Watermelon seed.
4. application according to claim 3, it is characterised in that：The citrulline administration concentration is 0.1~0.8mmol/L.
5. application according to claim 3, it is characterised in that：The citrulline administration amount is applied for every 50 vegetable seeds
Measure as 1~3mL；The salt stress environment is that NaCl concentration is 130~140mmol/L.
6. according to the application described in any one of Claims 1 to 5, it is characterised in that：The application process comprises the following steps：
(1) vegetable seeds of mature and plump is chosen, first with sterilization 10~15min of liquid disinfectant, then with aseptic water washing vegetable seeds；
(2) vegetable seeds for handling step (1) well soaks 4~6h under salt stress with citrulling solution, then by vegetable seeds
It is placed in the culture dish for being covered with filter paper, culture dish is placed in 28~32 DEG C of insulating box and cultivated；
(3) the citrulling solution is applied in culture dish, changed once every 22~28h, repeated 3~5 times.
7. application according to claim 6, it is characterised in that：The thimerosal is that mass concentration is 0.25~0.35%
Liquor potassic permanganate.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710797746.3A CN107493726B (en) | 2017-09-06 | 2017-09-06 | Application of citrulline in improving salt stress resistance of plant seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710797746.3A CN107493726B (en) | 2017-09-06 | 2017-09-06 | Application of citrulline in improving salt stress resistance of plant seeds |
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| Publication Number | Publication Date |
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| CN107493726A true CN107493726A (en) | 2017-12-22 |
| CN107493726B CN107493726B (en) | 2020-06-23 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109220076A (en) * | 2018-08-14 | 2019-01-18 | 江苏沿海地区农业科学研究所 | A kind of Sesbania seed processing method |
| CN112020930A (en) * | 2020-07-31 | 2020-12-04 | 江苏沿海地区农业科学研究所 | Method for inducing rice to improve salt tolerance |
| CN113597916A (en) * | 2021-07-30 | 2021-11-05 | 山东省农业科学院 | Application of ATP in improving salt stress resistance of plants |
| CN113748934A (en) * | 2021-08-27 | 2021-12-07 | 江苏省农业科学院 | Application of citrulline in promoting fruit enlargement and quality improvement of melons and fruits |
-
2017
- 2017-09-06 CN CN201710797746.3A patent/CN107493726B/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109220076A (en) * | 2018-08-14 | 2019-01-18 | 江苏沿海地区农业科学研究所 | A kind of Sesbania seed processing method |
| CN112020930A (en) * | 2020-07-31 | 2020-12-04 | 江苏沿海地区农业科学研究所 | Method for inducing rice to improve salt tolerance |
| CN112020930B (en) * | 2020-07-31 | 2021-10-08 | 江苏沿海地区农业科学研究所 | Method for inducing rice to improve salt tolerance |
| CN113597916A (en) * | 2021-07-30 | 2021-11-05 | 山东省农业科学院 | Application of ATP in improving salt stress resistance of plants |
| CN113748934A (en) * | 2021-08-27 | 2021-12-07 | 江苏省农业科学院 | Application of citrulline in promoting fruit enlargement and quality improvement of melons and fruits |
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| Publication number | Publication date |
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| CN107493726B (en) | 2020-06-23 |
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