CN109220076A - A kind of Sesbania seed processing method - Google Patents
A kind of Sesbania seed processing method Download PDFInfo
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- CN109220076A CN109220076A CN201810952713.6A CN201810952713A CN109220076A CN 109220076 A CN109220076 A CN 109220076A CN 201810952713 A CN201810952713 A CN 201810952713A CN 109220076 A CN109220076 A CN 109220076A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/30—Derivatives containing the group >N—CO—N aryl or >N—CS—N—aryl
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention belongs to field of agricultural cultivation, and in particular to a kind of Sesbania seed processing method, comprising the following steps: seed is immersed in 3% hydrogen peroxide and handles 20min by the Sesbania seed for (1) selecting full uniform no disease and pests harm；(2) Sesbania seed is cleaned to immerse in the concentrated sulfuric acid after drying and handles 20min, after immersion, outwell the concentrated sulfuric acid immediately, and rinse 2h under flowing water；(3) Sesbania seed is taken out and is immersed in seed-soaking liquid after cleaning, Sesbania seed and seed-soaking liquid are stirred using blender, mixing time 20min, stirring rate 30r/min, Sesbania seed 1h is impregnated after stirring, and Sesbania seed is laid on shield and is dried.Sesbania seed processing method of the present invention can significantly improve sesbania percentage of seedgermination under condition of salt stress.
Description
Technical field
The invention belongs to field of agricultural cultivation, and in particular to a kind of Sesbania seed processing method can significantly improve the salt side of body
Sesbania seed germination percentage under the conditions of compeling.
Technical background
According to incompletely statistics, there are about the natural salt dirting soil gross areas 1.0 × 10 in the whole world9hm2, and Secondary salinization soil area reaches
2.75×109hm2, Jiangsu accounts for about 1/4.With the continuous expansion of salinization soil area, salinity, which has become, influences land resource benefit
Significant obstacle factor seriously inhibits the performance of soil productivity, causes soil production efficiency relatively low, restricts agricultural
Development.
Sesbania is annual leguminous plant, and cauline leaf is in great numbers, and biomass is big, and it is excellent green manure and feeding that protein content is high
Use crop.Sesbania has stronger resistance, and soil suitability is extremely strong, can give birth on acidity, alkalinity, saline-alkali soil, nonirrigated farmland, flood ground
Long, well developed root system, root nodule is more, and nitrogen fixing capacity is strong, can be used as the pionner of soil remediation and alkaline land improvement, by salt
Sesbania is reasonably planted to alkali, can reach the purpose to alkaline land soil comprehensive improvement.In addition, Sesbania seed may further be used to produce
Sesbania gum is used for oil extraction in oil field, ore dressing metallurgy, mining blasting, weaving and daily-use chemical industry, is that natural plant uses crop；But field
Cyanines seed is thicker, and there are waxes on surface, not hygroscopic, if Sesbania seed is in salt stress item without broadcasting preceding seed treatment
The problems such as germination percentage declines, upgrowth situation is bad is easy to appear under part.
The present invention can significantly improve under condition of salt stress in view of the above-mentioned problems, provide a kind of Sesbania seed processing method
Sesbania seed germination percentage.
Summary of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of Sesbania seed processing methods.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of Sesbania seed processing method, comprising the following steps:
(1) seed is immersed in 3% hydrogen peroxide and handles 20min by the Sesbania seed for selecting full uniform no disease and pests harm；
(2) Sesbania seed is cleaned to immerse in the concentrated sulfuric acid after drying and handles 20min, after immersion, outwell dense sulphur immediately
Acid, and 2h is rinsed under flowing water；
(3) Sesbania seed is taken out and is immersed in seed-soaking liquid after cleaning, Sesbania seed and seed-soaking liquid are carried out using blender
Stirring, mixing time 20min, stirring rate 30r/min impregnate Sesbania seed 1h, Sesbania seed are put down after stirring
It is laid on shield and dries.
Before Sesbania seed sowing, Sesbania seed and sesbania special fertilizer are sowed after mixing.
The weight ratio of Sesbania seed and sesbania special fertilizer is preferably 1: 3 in the step (3).
The raw material of the sesbania special fertilizer includes the component of following parts by weight: 20 parts of pine soil, 20 parts of animal wastes, and mistake
2 parts and 0.1 part of sodium alginate of calcium phosphate.
The sesbania special fertilizer the preparation method comprises the following steps: by 20 parts of pine soil, 20 parts of animal wastes, 0.1 part of sodium alginate with
20 parts of rice washing waters are uniformly mixed according to above-mentioned weight part ratio, are stacked at after fermenting 10 days in 50 DEG C of greenhouses, are transferred to 40
It ferments 20 days in DEG C environment, is finally uniformly mixed with 2 parts of peroxophosphoric acid sodium, sesbania special fertilizer can be obtained.
Contain for every liter in the seed-soaking liquid: gibberellin 200mg, brassin lactones 200mg, citrulling 10mg, chlorination are blood red
Plain 0.05mg.
Seed-soaking liquid temperature is preferably 50 DEG C in the step (3).
Seed-soaking liquid weight is 5 times of Sesbania seed weight in the step (3).
Concentrated sulfuric acid mass fraction is 90% in the step (2).
Compared with prior art, the invention has the benefit that
Hydrogen peroxide also has the function of that promotion seed is sprouted in addition to the effect of seed disinfection under concentration appropriate, it
Seed permeability can be improved, the metabolism of seed can also be stimulated, improve the enzyme activity of Their Seed Germinating Period with mild corrosion kind skin
Property, play a significant role to seed dormancy is released.
Sesbania seed seed coat surface has coating of wax matter, plants skin like keratin, is not easy to be waterlogged.Kind skin accounts for seed weight
20% or so.Outmost one layer is epidermis, the palisade cell layer being made of single-row columnar cell, and cell wall has thickening existing
As.Therefore, Sesbania seed has strong property, and the present invention eliminates hard in such a way that dense sulfuric acid treatment and seed-soaking liquid stirring impregnate
Real kind of skin, shortens the residence time in the soil of Sesbania seed, prevents salinity in salt-soda soil from damaging Sesbania seed, improves germination
Rate shortens germinating time, is conducive to Sesbania seed and emerges ahead of time.
Gibberellin can make well developed root system, and pre- disease prevention, can promote axis, leaf growth significantly；Hemin is
The purification form of natural ferroheme, typically separates from animal blood, purifies out.It is recent the study found that
The hemin of certain concentration is alternatively arranged as plant growth regulator, carries out immersion treatment to plant, plant tissue or seed,
It can induce and gradually discharge total concentration≤1000ppm CO gas in plant, so as to improve the resistance of plant.The present invention
Hemin is used for the seed soaking of Sesbania seed for the first time, it is found that it has good facilitation to the sprouting of Sesbania seed.
In addition, test data through the invention also it can be seen that, hemin and other growth hormone have preferable synergistic effect,
The sprouting of Sesbania seed under condition of salt stress can be effectively facilitated.
Brassin lactones are a kind of new green environment protection plant growth regulator, are soaked by suitable concentration brassin lactones
Kind and treatment of stem and leaf spraying.Can promote the plant growths such as vegetables, melon, fruit, can improving quality, improve yield, color is gorgeous
Beautiful, blade is more thick and solid.The application's the study found that the brassin lactones of certain concentration can effectively improve the salt tolerant of Sesbania seed
Property, to promote the sprouting of Sesbania seed under condition of salt stress.
Citrulling is a kind of a-amino acid, be generated in urea cycle from ornithine and carbamoyl phosphate, or
The by-product that arginine generates NO is catalyzed by nitricoxide synthase (NOS).The application's the study found that certain concentration melon ammonia
Acid can effectively improve the salt tolerance of Sesbania seed, to promote the sprouting of Sesbania seed under condition of salt stress.
In addition, gibberellin, brassin lactones, citrulling and hemin have stronger synergistic effect, four kinds of components
It is used in conjunction with the sprouting that can more effectively promote Sesbania seed under condition of salt stress.
It is rich in organic matter and various nutrients in sesbania special fertilizer, and fertilizer efficiency is long and stablize, and can increase alkaline land soil
The content of middle organic matter promotes the formation of alkaline land soil crumb structure, increases alkaline land soil water conservation, ventilative and fertilizer conservation energy
Power provides good growing environment for Sesbania seed, and turning over makes moisture in alkaline land soil, nutrient, air and heat etc. more
Uniformly coordinate, be conducive to increase soil fertility, provides good condition for sowing.
Sesbania special fertilizer of the present invention is formed by many kinds of substance mixed configuration, and allocation ratio provided by the invention can
It is set mutually to maximize favourable factors and minimize unfavourable ones.Pine soil has heat-insulated, moisturizing and the effect for reducing weeds propagation and widespread.Pine soil be pine needle ferment and
At, it is more containing humus, it is light, pine soil permeability is very good, seepage of water is strong, contains a large amount of organic matters, and have certain anti-
Worm and disease act on；The use of sodium alginate is conducive to various composition and is uniformly adhered to around seed, constantly mentions for seed
For nutrient.With raw material provided by the present invention and proportional arrangement sesbania special fertilizer, and with seed mixed seeding, can for sesbania sprout
Stable rhizospheric environment condition is provided with growth, effectively supplements nutrient needed for sesbania growth course.
Sesbania is more sensitive to the reaction of phosphate fertilizer, and phosphate fertilizer content is less in alkaline land soil, therefore applies phosphate fertilizer and cooperate potassium
Fertilizer can effectively increase sesbania biomass；Calcium ion is mineral nutrient element necessary to plant growth, and a kind of important generation
Thank to Auto-regulator.The work of amylase in Seed Germination, lipase, glutamic-pyruvic transaminase can be improved in the addition of exogenous calcium
Property, and then promote the sprouting of seed.Calcium superphosphate is common cheap chemical phosphatic ferfilizer, calcium superphosphate and pine soil in agricultural production
It is used in mixed way with organic fertilizer made of animal wastes co-fermentation, contact of the calcium superphosphate with soil can be reduced, be effectively prevented from
Titanium pigment becomes indissoluble object and reduces fertilizer efficiency.Calcium superphosphate and organic fertilizer agglomerate form loose agglomerate after mixing application soil,
Moisture easily penetrates and dissolves Leaching Properties of Soluble Phosphorus, and root system of plant is also easy to protrude into the phosphorus that agglomerate absorbed inside is dissolved in water.Calcium superphosphate
It mixes and applies with organic fertilizer, also become single fertilising and increase the element species for being applied to plant into compound fertilising, and promote plant pair again
Phosphorus is absorbed and utilized, and has better meet crop nutritional need.After calcium superphosphate and organic fertilizer mix application soil, well-established law can be overcome to apply
The normal life and activity for influencing Soil Microorganism after phosphorus because of the phosphorous excessive concentration of soil, inhibit Plant To Nutrient element
The drawbacks such as absorption, protect the activity of soil, also improve the absorption efficiency of Plant To Nutrient element.
Specific embodiment
Embodiment 1
The Sesbania seed for selecting 900 full uniform no disease and pests harms, is divided into 3 groups, respectively according to seed treatment described below
Method handles Sesbania seed.Two layers of filter paper is put in culture dish, the NaCl that 8mL concentration is 200mmol/L is added in each culture dish
Solution, 100 seeds of each processing, 3 repetitions.Set germination (25 DEG C of constant temperature, illumination 16h) in the light incubator.Daily and
When supplement salting liquid, the sprouting situation in Seed Germination per 1 seed of observational record for 24 hours reaches seed with radicle length
The standard that the 1/2 of length is sprouted as seed, measurement germination percentage, germinating energy and germination index.The results are shown in Table 1.
First group of Sesbania seed is not handled；
Second group of method for treating seeds is referring to record in CN104956809A (processing method before Sesbania seed sowing)
The prior art: this method comprises the following steps: 1) dense sulfuric acid treatment: Sesbania seed is dipped in the concentrated sulfuric acid, handles 5min, is taken out
It cleans；2) warm water is handled: will treated that seed is soaked in 40 DEG C of warm water for 24 hours by step 1)；3) gibberellin (GA) is handled:
Will by step 2), treated that seed is soaked in GA solution, handle 4h, take out and clean；4) at the basic element of cell division (6-BA)
Reason: will by step 3), treated that seed is soaked in 6-BA solution, handle 4h, take out and clean；5) calcium nitrate-hydrogen peroxide
Solution processing: by step 4), treated that seed is soaked in Ca (NO3)2-H2O2In solution for 24 hours, taking-up is dried.
Third group method for treating seeds are as follows: seed is immersed 3% by the Sesbania seed for (1) selecting full uniform no disease and pests harm
Hydrogen peroxide in handle 20min；(2) Sesbania seed is cleaned to immerse in 90% concentrated sulfuric acid after drying and handles 20min, immersion terminates
Afterwards, the concentrated sulfuric acid is outwelled immediately, and rinses 2h under flowing water；(3) Sesbania seed is taken out in the seed-soaking liquid for immersing 50 DEG C after cleaning,
The seed-soaking liquid weight is 5 times of Sesbania seed weight, is stirred using blender to Sesbania seed and seed-soaking liquid, mixing time
For 20min, stirring rate 30r/min, Sesbania seed 1h is impregnated after stirring, Sesbania seed is laid on shield and is dried in the air
It is dry.Contain for every liter in the seed-soaking liquid: gibberellin 200mg, brassin lactones 200mg, citrulling 10mg, hemin
0.05mg。
Calculation formula:
Percentage of seedgermination (%)=(M7/ M) × 100%
Potentiality of seed (%)=(M3/ M) × 100%
Germination index=∑ Gt/Dt
In formula: Gt is the germinative number in the t time, and Dt is corresponding germination number of days
M1、M2、M3、M4、M5、M6、M7--- after planting 1-7 days seeds germinate grain number daily；
M --- for planting experimentally seed number
Table 1
Above-mentioned data are analyzed it is found that salt can be effectively improved using Sesbania seed processing method of the present invention
Percentage of seedgermination, germinating energy and germination index under stressful environmental.
Embodiment 2
Field plot trial is carried out in the area Yancheng City great Feng of the Jiangsu Province farm Jin Hai, soil salt content 0.3%, organic matter
Content 1.1%, belongs to moderate saline-alkali soil block, selects 9 cells of 8m × 8m, is divided into three groups, every group of 3 repetition, respectively according to
Method for treating seeds described below handles Sesbania seed.Sowing measured emergence rate after 45 days, and select in every group of sesbania growing way compared with
For 5 plants of uniform plant, average plant height, stem thickness, overground part dry weight and underground part dry weight are measured, the results are shown in Table 2.
First group of Sesbania seed is not handled；
Second group of method for treating seeds is referring to record in CN104956809A (processing method before Sesbania seed sowing)
The prior art: this method comprises the following steps: 1) dense sulfuric acid treatment: Sesbania seed is dipped in the concentrated sulfuric acid, handles 5min, is taken out
It cleans；2) warm water is handled: will treated that seed is soaked in 40 DEG C of warm water for 24 hours by step 1)；3) gibberellin (GA) is handled:
Will by step 2), treated that seed is soaked in GA solution, handle 4h, take out and clean；4) at the basic element of cell division (6-BA)
Reason: will by step 3), treated that seed is soaked in 6-BA solution, handle 4h, take out and clean；5) calcium nitrate-hydrogen peroxide
Solution processing: by step 4), treated that seed is soaked in Ca (NO3)2-H2O2In solution for 24 hours, taking-up is dried.
Third group method for treating seeds are as follows: seed is immersed 3% by the Sesbania seed for (1) selecting full uniform no disease and pests harm
Hydrogen peroxide in handle 20min；(2) Sesbania seed is cleaned to immerse in 90% concentrated sulfuric acid after drying and handles 20min, immersion terminates
Afterwards, the concentrated sulfuric acid is outwelled immediately, and rinses 2h under flowing water；(3) Sesbania seed is taken out in the seed-soaking liquid for immersing 50 DEG C after cleaning,
The seed-soaking liquid weight is 5 times of Sesbania seed weight, is stirred using blender to Sesbania seed and seed-soaking liquid, mixing time
For 20min, stirring rate 30r/min, Sesbania seed 1h is impregnated after stirring, Sesbania seed is laid on shield and is dried in the air
It is dry, Sesbania seed and sesbania special fertilizer are sowed after mixing according to weight ratio 1: 3.The preparation method of the sesbania special fertilizer
Are as follows: by 20 parts of pine soil, 20 parts of animal wastes, 0.1 part of sodium alginate with 20 parts of rice washing waters according to above-mentioned weight part ratio
Be uniformly mixed, be stacked at after fermenting 10 days in 50 DEG C of greenhouses, be transferred in 40 DEG C of environment and ferment 20 days, finally with 2 parts of peroxophosphoric acid
Sodium is uniformly mixed, and can obtain sesbania special fertilizer.Contain for every liter in the seed-soaking liquid: gibberellin 200mg, brassin lactones 200mg,
Citrulling 10mg, hemin 0.05mg.
Table 2
It is analyzed by above-mentioned data it is found that Sesbania seed processing method of the present invention makes sesbania upgrowth situation good,
Biomass is larger.
Embodiment 3
Field plot trial is carried out in the area Yancheng City great Feng of the Jiangsu Province farm Jin Hai, soil salt content 0.3%, organic matter
Content 1.1%, belongs to moderate saline-alkali soil block, selects 9 cells of 8m × 8m, is divided into three groups, every group of 3 repetition, respectively according to
3 groups of method for treating seeds in embodiment 2 are handled.The disease rate that sesbania plant is measured after sowing 45 days, as a result such as 3 institute of table
Show.
Table 3
It is analyzed by above-mentioned data it is found that Sesbania seed processing mode provided by the present invention can significantly reduce sesbania disease
Rate.
Embodiment 4
The Sesbania seed for selecting 900 full uniform no disease and pests harms, is divided into 3 groups, respectively according to 3 groups of kinds in embodiment 1
Subprocessing method is handled.Two layers of filter paper is put in culture dish, it is -0.5MPa that each culture dish, which is separately added into 8mL osmotic pressure,
PEG solution, 100 seeds of each processing, 3 repetitions.Set germination (25 DEG C of constant temperature, illumination 16h) in the light incubator.Often
PEG solution is replenished in time in it, and the sprouting situation of every 1 seed of observational record for 24 hours, is reached in Seed Germination with radicle length
The standard that the 1/2 of seed length is sprouted as seed measures germination percentage.The results are shown in Table 4.
Table 4
Above-mentioned data are analyzed it is found that using Sesbania seed processing method of the present invention, in drought stress tune
Percentage of seedgermination can also be effectively improved under section.
Embodiment 5
The Sesbania seed for selecting 1500 full uniform no disease and pests harms, is divided into 5 groups, according to third group in embodiment 1
Method for treating seeds is handled, and the difference between 5 groups is the time using 90% dense sulfuric acid treatment.Two are put in culture dish
The NaCl solution that 8mL concentration is 200mmol/L, 100 seeds of each processing, 3 repetitions is added in layer filter paper, each culture dish.
Set germination (25 DEG C of constant temperature, illumination 16h) in the light incubator.Salting liquid is replenished in time daily, per for 24 hours in Seed Germination
The sprouting situation of 1 seed of observational record reaches 1/2 standard sprouted as seed of seed length, measurement using radicle length
Germination percentage.The results are shown in Table 5.
Table 5
Above-mentioned data are analyzed it is found that being coerced using processing 20min in 90% concentrated sulfuric acid in salt before Sesbania seed sowing
It is higher to compel germination percentage under environment.
Embodiment 6
The Sesbania seed for selecting 2700 full uniform no disease and pests harms, is divided into 9 groups, according to third group in embodiment 1
Method for treating seeds is handled, and the difference between 9 groups is the component of every liter of seed-soaking liquid.Two layers of filter paper is put in culture dish,
The NaCl solution that 8mL concentration is 200mmol/L, 100 seeds of each processing, 3 repetitions is added in each culture dish.It is placed in illumination
(25 DEG C of constant temperature, illumination 16h) is germinateed in incubator.Salting liquid, every observational record for 24 hours in Seed Germination are replenished in time daily
The sprouting situation of 1 seed is reached 1/2 standard sprouted as seed of seed length using radicle length, measures germination percentage.Knot
Fruit is as shown in table 6.
Table 6
Above-mentioned data are analyzed it is found that containing when in every liter of seed-soaking liquid: gibberellin 200mg, brassin lactones
When 200mg, citrulling 10mg, hemin 0.05mg, Sesbania seed germination percentage under salt stress environment is higher.
Embodiment 7
Field plot trial is carried out in the area Yancheng City great Feng of the Jiangsu Province farm Jin Hai, soil salt content 0.3%, organic matter
Content 1.1% belongs to moderate saline-alkali soil block, selects 9 cells of 8m × 8m, is divided into 9 groups, every group of 3 repetition, according to implementation
The method for treating seeds of third group is handled in example 2, and 9 groups of difference is the raw material components of sesbania special fertilizer.Sowing 45 days
The emergence rate of sesbania plant is measured afterwards, and the results are shown in Table 7.
Table 7
Above-mentioned data are analyzed it is found that when the raw material of sesbania special fertilizer includes the component of following parts by weight: pine needle
20 parts of soil, 20 parts of animal wastes, 2 parts of calcium superphosphate and at 0.1 part of sodium alginate, Sesbania seed emergence rate under salt stress environment
It is higher.
Embodiment 8
Field plot trial is carried out in the area Yancheng City great Feng of the Jiangsu Province farm Jin Hai, soil salt content 0.3%, organic matter
Content 1.1% belongs to moderate saline-alkali soil block, selects 5 cells of 8m × 8m, is divided into 5 groups, every group of 3 repetition, according to implementation
The method for treating seeds of third group is handled in example 2, and 5 groups of difference is the weight ratio of Sesbania seed Yu sesbania special fertilizer.
The emergence rate of sesbania plant is measured after sowing 45 days, the results are shown in Table 8.
Table 8
Above-mentioned data are analyzed it is found that when the weight ratio of Sesbania seed and sesbania special fertilizer is 1: 3, Sesbania seed
Emergence rate is higher under salt stress environment.
Claims (9)
1. a kind of Sesbania seed processing method, comprising the following steps:
(1) seed is immersed in 3% hydrogen peroxide and handles 20min by the Sesbania seed for selecting full uniform no disease and pests harm；
(2) Sesbania seed is cleaned to immerse in the concentrated sulfuric acid after drying and handles 20min, after immersion, outwell the concentrated sulfuric acid immediately, and
2h is rinsed under flowing water；
(3) Sesbania seed is taken out and is immersed in seed-soaking liquid after cleaning, Sesbania seed and seed-soaking liquid are stirred using blender,
Mixing time is 20min, stirring rate 30r/min, and Sesbania seed 1h is impregnated after stirring, Sesbania seed is laid in pad
It is dried on cloth.
2. Sesbania seed processing method according to claim 1, which is characterized in that, will be by power before Sesbania seed sowing
Benefit requires the Sesbania seed after 1 step process to sow after mixing with sesbania special fertilizer.
3. Sesbania seed processing method according to claim 2, which is characterized in that in the step (3) Sesbania seed with
The weight ratio of sesbania special fertilizer is preferably 1: 3.
4. Sesbania seed processing method according to claim 2, which is characterized in that the raw material of the sesbania special fertilizer includes
The component of following parts by weight: 20 parts of pine soil, 20 parts of animal wastes, 2 parts and 0.1 part of sodium alginate of calcium superphosphate.
5. Sesbania seed processing method according to claim 2, which is characterized in that the preparation method of the sesbania special fertilizer
Are as follows: by 20 parts of pine soil, 20 parts of animal wastes, 0.1 part of sodium alginate with 20 parts of rice washing waters according to above-mentioned weight part ratio
Be uniformly mixed, be stacked at after fermenting 10 days in 50 DEG C of greenhouses, be transferred in 40 DEG C of environment and ferment 20 days, finally with 2 parts of peroxophosphoric acid
Sodium is uniformly mixed, and can obtain sesbania special fertilizer.
6. Sesbania seed processing method according to claim 1, which is characterized in that contain for every liter in the seed-soaking liquid: red
Mycin 200mg, brassin lactones 200mg, citrulling 10mg, hemin 0.05mg.
7. Sesbania seed processing method according to claim 1, which is characterized in that seed-soaking liquid temperature in the step (3)
Preferably 50 DEG C.
8. Sesbania seed processing method according to claim 1, which is characterized in that seed-soaking liquid weight in the step (3)
It is 5 times of Sesbania seed weight.
9. Sesbania seed processing method according to claim 1, which is characterized in that concentrated sulfuric acid quality in the step (2)
Score is 90%.
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Cited By (2)
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| CN109392349A (en) * | 2018-12-26 | 2019-03-01 | 扬州大学 | A kind of tidal flat of Jiangsu Province reparation plantation sesbania method |
| CN115710589A (en) * | 2022-11-21 | 2023-02-24 | 隆平生物技术(海南)有限公司 | Genetic transformation method of sesbania |
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