CN107347605A - A kind of application of epiphysin in peanut salt damage stress is alleviated - Google Patents
A kind of application of epiphysin in peanut salt damage stress is alleviated Download PDFInfo
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- CN107347605A CN107347605A CN201710654534.XA CN201710654534A CN107347605A CN 107347605 A CN107347605 A CN 107347605A CN 201710654534 A CN201710654534 A CN 201710654534A CN 107347605 A CN107347605 A CN 107347605A
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- nutrient solution
- sodium chloride
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- epiphysin
- peanut
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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Abstract
The invention discloses a kind of application of epiphysin in peanut salt damage stress is alleviated, step is：A) experiment process：Choose peanut seed to be seeded in vermiculite, be changed to water planting in high 6~8 cm of the cm of hypocotyl length 3~4, stem, select growing way consistent peanut seedling during transplanting, plant in plastics field planting plate with holes, be placed on hydroponic box, root is immersed in Huo Gelan nutrient solutions；B) it is respectively processed after water planting, during the nutrient solution renewed：Normal growth in Huo Gelan nutrient solutions；Sodium chloride is added in nutrient solution；Sodium chloride and epiphysin are added in nutrient solution；Sodium chloride and epiphysin are added in nutrient solution；Sodium chloride and epiphysin are added in nutrient solution；Sodium chloride and epiphysin are added in nutrient solution；C) peanut seedling character mutation is observed after handling, determines the whole strain fresh weight of peanut seedling, then fixing, drying.It is easy to implement the method, strong operability, it is possible to increase the biomass of peanut seedling under salt stress, improve peanut seedling salt tolerance.
Description
Technical field
The present invention relates to peanut production field, is more particularly to a kind of use of epiphysin in peanut salt damage stress is alleviated
On the way.Growth-regulating hormone plays very important effect in terms of Physiological effect, increase stress resistance of plant, and external source is taken in this research
Addition epiphysin handles the ability for alleviate infringement of the salt damage to peanut seedling, assigning peanut resistance poor environment.
Background technology
The soil salinization is to influence the serious problems of agricultural production and ecological environment, and according to statistics, there is 20% arable land in the whole world
By different degrees of salination, and China saline-alkali soil area about 1.0 × 107hm2, the 6.2% of cultivated area is accounted for, is had at present
An important factor for 80% or so is not yet developed, and the salinization of soil is limiting plant growth, has a strong impact on plant growth,
Reduce crop yield.Peanut (Arachis hypogaea) is important oil crops and industrial crops, and its oil content is up to
50%, there is important nutritive value and economic value.Oil crops strive the contradiction on ground with cereal crops in recent years,
In order to not strive ground with grain, the renovation and utilization in salt-soda soil is increased, for expanding peanut cultivation area, improves pattern of farming, increase wound
Receive and ensure that edible oil has important practical significance safely.
Epiphysin (melatonin, MT) is a kind of indole hormone being widely present in animal and plant body, and chemical name is
Melatonin, it is widely present in animals and plants.Epiphysin has multiple functions in plant, including regulation is planted
Thing grows, and such as promotes the growth of lateral root, changes the florescence, participates in Chlorophyll synthesis and Delaying Leaf-Senescence.In addition,
Epiphysin can also strengthen plant and think that epiphysin is to planting to arid, salt damage and the resistance for a variety of adverse circumstances such as damaging to plants caused by sudden drop in temperature, at present research
The degeneration-resistant adjustment effect of thing is mainly due to the removing that it participates in active oxygen.At present, relevant epiphysin is to peanut children under salt stress
The relieving effect of seedling has not been reported, and this research takes external source addition epiphysin processing to alleviate wound of the salt damage to peanut seedling
Evil, the new way of a safe noresidue is provided for agriculture chemistry salt resistance.
The content of the invention
The purpose of the present invention be the provision of a kind of epiphysin alleviate peanut seedling salt damage stress in application, method
Simply, strong operability, it can effectively improve peanut seedling biomass, alleviate the injury that salt damage is brought to peanut seedling, show
Write the salt resistance ability for improving peanut.
In order to realize above-mentioned purpose, the present invention uses following technical measures：
A kind of application of epiphysin in peanut seedling salt damage stress is alleviated, its step are：
The structural formula of described epiphysin is：
A) experiment process：Choose full peanut seed to be seeded in vermiculite, it is high by 6 to be about 3~4cm, stem in hypocotyl
It is changed to water planting during~8cm, selects growing way consistent peanut seedling during transplanting, plant in plastics field planting plate with holes, be placed on 2.5L
On hydroponic box, 2L nutrient solutions are added in hydroponic box, root is immersed in Huo Gelan nutrient solutions；
B) for water planting after 7~10 days, every basin carries out following several processing respectively when changing the new nutrient solutions of 2L：Huo Gelan is cultivated
Normal growth (CK1) in liquid；1% (20g) sodium chloride (CK2) is added in nutrient solution；1% (20g) sodium chloride is added in nutrient solution
With 50umol/L epiphysins (NaCl+50M)；1% (20g) sodium chloride and 100umol/L epiphysins (NaCl+ are added in nutrient solution
100M)；Added in nutrient solution in 1% (20g) sodium chloride and 150umol/L epiphysins (NaCl+150M), nutrient solution and add 1%
(20g) sodium chloride and 200umol/L epiphysins (NaCl+200M).It is black that taking off for sodium chloride and various concentrations is added in nutrient solution
Element, observe under salt stress, mitigation of the epiphysin to peanut seedling, and further verify addition epiphysin optimum concentration model
Enclose.
Peanut seedling character mutation is observed when 5~10 days after c) handling.And determine the whole strain fresh weight of peanut seedling, with after
105 DEG C fixing 28-32min, 78-82 DEG C be dried to constant weight after claim its dry weight.It was found that under salt stress, taking off for various concentrations is added
The growing way of melanocyte peanut seedling plant is better than CK2, illustrates the epiphysin of above-mentioned several concentration and has not to peanut seedling salt stress
With the mitigation of degree.
By abovementioned technology, solve peanut and plant the difficulty of long potential difference in salt-soda soil, improve biomass,
Enhance the ability of plant salt tolerant.It is more convenient that its invention operates compared with prior art, and effect is more notable.
The present invention compared with prior art, has advantages below and effect：
Epiphysin is used to alleviate in peanut salt damage stress by the present invention, and application method is simple and easy, workable, the party
Method can significantly improve peanut seedling salt resistance ability.Significantly improve the ability of peanut plant resistance salt stress.In salt stress bar
Under part, by external source apply epiphysin can peanut seedling plant fresh weight and dry weight increase 23%- respectively compared to CK2
50% and 20%-52%.
Brief description of the drawings
Fig. 1 is that epiphysin handles 7d schematic diagrames under a kind of salt stress.
CK1 is control；CK2 is salt treatment, i.e., 1% sodium chloride is only added in water planting liquid；NaCl+50M is in nutrient solution
Add 1% sodium chloride and 50umol/L；1% sodium chloride and 100umol/L epiphysins are added in NaCl+100M nutrient solutions；NaCl+
150M is to add 1% sodium chloride and 150umol/L epiphysins in nutrient solution；NaCl+200M is to add 1% sodium chloride in nutrient solution
With 200umol/L epiphysins.The peanut seedling growing way that epiphysin is handled under salt stress is substantially better than the peanut seedling under salt stress.
Embodiment
The present invention is expanded on further with reference to specific embodiment, but the present invention is not limited to following examples.
Embodiment 1：
With reference to embodiment, the present invention will be further described, material therefor, such as epiphysin, and NaCl and vermiculite soil are
To be commercially available, mill water culture nutrient solution is formulated by Huo Gelan nutrient solution prescriptions.
A kind of application of epiphysin in peanut salt damage stress is alleviated, its step are：
A, the small side's alms bowl (10 × 10 × 8.5cm) of black fills vermiculite soil, with water saturates vermiculite.Full seed is chosen, without broken
The peanut seed of damage, first handle 20min with liquor natrii hypochloritis, then with distilled water flushing 3 times, choose good peanut kind of germinateing
It is some, sow in vermiculite soil and cultivated at 20 DEG C, seedling cotyledon deploys after about 7 days, and 3 or 4cm, stem are about in hypocotyl
Water planting is changed to during high 6 or 8cm；Water planting plastic tub volume is about 2.5L, loads Huo Gelan nutrient solution about 2L, each blue plastic tub
There is the foam cap of a manual manufacture, diameter about 1cm hole is equably stamped on each lid, each aperture moves into one plant of peanut
Seedling.During transplanting, the consistent peanut seedling absorbent cotton of growing way is colonized in foam cap aperture, made around Liang Quan in root neck
Young root extend into 2 or 3cm in nutrient solution.After peanut transplanting, the photoperiod is placed on as 16h on daytime, 25 DEG C/night 8h, 22 DEG C of temperature
Cultivated 7 or 8 or 9 or 10 days in room.
B, salt stress and melatonin change the new nutrient solutions of 2L after peanut seedling is transplanted 7 or 8 or 9 or 10 days in every basin
When add.It is divided into following groups：Normal growth (CK1) in Huo Gelan nutrient solutions；1% (20g) sodium chloride is added in nutrient solution
(CK2)；1% (20g) sodium chloride and 50umol/L epiphysins (NaCl+50M) are added in nutrient solution；1% is added in nutrient solution
(20g) sodium chloride and 100umol/L epiphysins (NaCl+100M)；1% (20g) sodium chloride and 150umol/L are added in nutrient solution
1% (20g) sodium chloride and 200umol/L epiphysins (NaCl+200M) are added in epiphysin (NaCl+150M), nutrient solution.Seeking
The epiphysin of sodium chloride and various concentrations is added in nutrient solution, is observed under salt stress, mitigation of the epiphysin to peanut seedling, and
The optimum concentration scope of peanut seedling salt damage can be alleviated by further having verified epiphysin.
C, treatment fluid specifically matches：
Peanut seedling character mutation is observed when 5 or 7 or 9 or 10 days after D, handling, and determines the whole strain fresh weight of peanut seedling, with
After 105 DEG C finish 28 or 29 or 30 or 31 or 32min, 78 or 79 or 80 or 81 or 82 DEG C be dried to constant weight after claim its dry weight.
Influence of the epiphysin to peanut seedling biomass under the salt stress of table 1
| Processing | Plant fresh weight | Plant weights |
| CK1 | 5.4318±0.0711 | 0.9183±0.0296 |
| CK2 | 3.495±0.0676 | 0.5194±0.0188 |
| NaCl+50M | 4.2922±0.1153 | 0.6434±0.0047 |
| NaCl+100M | 4.5283±0.0613 | 0.6864±0.0317 |
| NaCl+150M | 4.8515±0.2168 | 0.7298±0.0504 |
| NaCl+200M | 5.1734±0.2448 | 0.7832±0.0054 |
Under salt stress, addition 50,100,150, the peanut seedling of 200umol/L epiphysins are compared with CK2, plant fresh weight point
24.09%, 30.91%, 40.26%, 49.56% is not improved；Plant weights have been respectively increased 23.88%, 32.16%,
40.52%, 50.79%.
Above experimental data illustrates, under salt stress, external source, which applies epiphysin, can significantly improve peanut seedling increment, and
The effective injury alleviated salt stress and brought to Brassica Napus Seedling.
Claims (1)
1. a kind of application of epiphysin in peanut salt damage stress is alleviated, its step are：
A) experiment process：Choose peanut seed to be seeded in vermiculite, water is changed in the cm of hypocotyl length 3~4, high 6~8 cm of stem
Training, growing way consistent peanut seedling is selected during transplanting, plant in plastics field planting plate with holes, be placed on volume 2.5L hydroponic boxes,
2L nutrient solutions are added in hydroponic box, root is immersed in Huo Gelan nutrient solutions；
B) water planting is after 7~10 days, is respectively processed during the nutrient solution renewed：Normal growth in Huo Gelan nutrient solutions；Nutrient solution
1% sodium chloride of middle addition；1% sodium chloride and 50umol/L epiphysins are added in nutrient solution；Added in nutrient solution 1% sodium chloride and
100umol/L epiphysins；Added in nutrient solution added in 1% sodium chloride and 150umol/L epiphysins, nutrient solution 1% sodium chloride and
200umol/L epiphysins；
Peanut seedling character mutation is observed when 5~10 days after c) handling, the whole strain fresh weight of peanut seedling is determined, is killed with after 105 DEG C
Blue or green 28-32min, 78-82 DEG C be dried to constant weight after claim its dry weight.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110637812A (en) * | 2019-09-30 | 2020-01-03 | 河南科技学院 | Slow-release plant growth stimulator for delaying yellowing and aging of peanut leaves |
| CN110692640A (en) * | 2019-09-30 | 2020-01-17 | 河南科技学院 | Composite biostimulation agent for delaying senescence and death of peanut leaves in middle and later growth stages |
| CN116235753A (en) * | 2023-03-16 | 2023-06-09 | 中国科学院新疆生态与地理研究所 | Method for improving salt tolerance of cannabis sativa seedlings by utilizing exogenous hormone |
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| CN104350836A (en) * | 2014-11-11 | 2015-02-18 | 黑龙江八一农垦大学 | New application of melatonin to improving saline-alkaline stress resistance of plant seeds |
| CN104412979A (en) * | 2013-08-19 | 2015-03-18 | 中国科学院遗传与发育生物学研究所 | New applications of melatonin |
| CN104604864A (en) * | 2015-02-13 | 2015-05-13 | 中国农业大学 | Melatonin solution capable of improving anti-stress capability of plant |
| CN105900818A (en) * | 2016-07-07 | 2016-08-31 | 安徽科技学院 | Method for relieving salt stress for muskmelon seedlings |
| CN106069693A (en) * | 2016-07-05 | 2016-11-09 | 中国农业科学院油料作物研究所 | A kind of compound adjusting control agent is in the application alleviated during Brassica campestris L salt damage is coerced |
| CN106171583A (en) * | 2016-07-15 | 2016-12-07 | 中国农业科学院油料作物研究所 | A kind of 5 hydroxytryptamines are alleviating the application that Brassica campestris L salt damage is coerced |
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- 2017-08-03 CN CN201710654534.XA patent/CN107347605A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104412979A (en) * | 2013-08-19 | 2015-03-18 | 中国科学院遗传与发育生物学研究所 | New applications of melatonin |
| CN104350836A (en) * | 2014-11-11 | 2015-02-18 | 黑龙江八一农垦大学 | New application of melatonin to improving saline-alkaline stress resistance of plant seeds |
| CN104604864A (en) * | 2015-02-13 | 2015-05-13 | 中国农业大学 | Melatonin solution capable of improving anti-stress capability of plant |
| CN106069693A (en) * | 2016-07-05 | 2016-11-09 | 中国农业科学院油料作物研究所 | A kind of compound adjusting control agent is in the application alleviated during Brassica campestris L salt damage is coerced |
| CN105900818A (en) * | 2016-07-07 | 2016-08-31 | 安徽科技学院 | Method for relieving salt stress for muskmelon seedlings |
| CN106171583A (en) * | 2016-07-15 | 2016-12-07 | 中国农业科学院油料作物研究所 | A kind of 5 hydroxytryptamines are alleviating the application that Brassica campestris L salt damage is coerced |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110637812A (en) * | 2019-09-30 | 2020-01-03 | 河南科技学院 | Slow-release plant growth stimulator for delaying yellowing and aging of peanut leaves |
| CN110692640A (en) * | 2019-09-30 | 2020-01-17 | 河南科技学院 | Composite biostimulation agent for delaying senescence and death of peanut leaves in middle and later growth stages |
| CN116235753A (en) * | 2023-03-16 | 2023-06-09 | 中国科学院新疆生态与地理研究所 | Method for improving salt tolerance of cannabis sativa seedlings by utilizing exogenous hormone |
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Application publication date: 20171117 |