CN106068779A - A kind of method utilizing melatonin to strengthen the high temperature resistant storage of Herba bromi japonici Priming Seeds - Google Patents
A kind of method utilizing melatonin to strengthen the high temperature resistant storage of Herba bromi japonici Priming Seeds Download PDFInfo
- Publication number
- CN106068779A CN106068779A CN201610453578.1A CN201610453578A CN106068779A CN 106068779 A CN106068779 A CN 106068779A CN 201610453578 A CN201610453578 A CN 201610453578A CN 106068779 A CN106068779 A CN 106068779A
- Authority
- CN
- China
- Prior art keywords
- seed
- melatonin
- priming
- seedling
- high temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
- A01C1/025—Testing seeds for determining their viability or germination capacity
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01F—PROCESSING OF HARVESTED PRODUCE; HAY OR STRAW PRESSES; DEVICES FOR STORING AGRICULTURAL OR HORTICULTURAL PRODUCE
- A01F25/00—Storing agricultural or horticultural produce; Hanging-up harvested fruit
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Soil Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention belongs to seed germination and storage technique field, be specifically related to a kind of method utilizing melatonin to strengthen the high temperature resistant storage of Herba bromi japonici Priming Seeds.Comprise the following steps: (1) selects for examination oat seed；(2) adjustment of seed moisture content；(3) seed priming processes；(4) simulation high-temperature storage processes；(5) determination of the high temperature resistant holding conditions of melatonin Priming Seeds.The present invention can be as strengthening the high temperature resistant storage performance of Herba bromi japonici Priming Seeds, promoting seed germination and the effective ways of growth of seedling.
Description
Technical field
The invention belongs to seed germination and storage technique field, be specifically related to one and utilize melatonin to strengthen Herba bromi japonici initiation kind
The method of the high temperature resistant storage of son.
Background technology
Seed priming is that a kind of seed that controls slowly absorbs water and the preconditioning technique of progressively Hui Gan.In actual agricultural production
In, often use the aged seed preserved, and cause process that the vigor of aged seed can be made to be restored or improve, promote low
Seed germination under the adverse environmental factor such as warm, arid, saves seed consumption, lowers production cost.But, cause and be unfavorable for that seed is store
Hide, shorten seed longeivity, and then constrain its application in seed commodity production and sale.At present, both at home and abroad about initiation
The research of technology and application majority concentrate on it to be improved and raising aspect vegetable and Vigor of Crop, rarely have relate to causing right
Seed longeivity, storage potential and Priming Seeds sprouting and growth of seedling affect the research of aspect.
Melatonin (Melatonin, MT) is the indole derivatives of a kind of tryptophan, is found in the earliest 1917, its
Physiological action in animal body is furtherd investigate.In recent years, melatonin is detected in increasing plant,
The functional study of growth and development of plants and metabolism is the most increasingly paid close attention to by it by people.There are some researches show, melatonin in plant
There is removing free radical, regulation photoperiod, protection chlorophyll, strengthen the physiological functions such as stress resistance of plant.But, so far,
It is showed no both at home and abroad about melatonin Priming Seeds shelf-life and the report of Seed Germination.
Herba bromi japonici (Avena sativa L.) is grass family Avena annual Grain feed plant, mainly produces in China
In Qinghai, Gansu and Sichuan.Herba bromi japonici is tender as the high quality grass material of poultry, its leaf and straw succulence, good palatability, thick egg
In vain, crude fat content high, be of high nutritive value, crude fiber content is low, both can make green hay, and the most modulated ensilage, to solution
Certainly extremely frigid zones domestic animal forage grass demand uneven contradiction seasonal with forage grass supply is significant.Meanwhile, Herba bromi japonici drought resisting, anti-
The feature that inverse property is strong determines its adverse circumstances that can adapt to arid area.Additionally, as green nourishing health care crop, Herba bromi japonici
Containing a large amount of protein, water soluble dietary fiber, vitamin and mineral in seed, fat content is higher, and linoleic acid content
Height, most important to human health.
In China, along with fast development and the continuous expansion of raising dairy cattle scale, the Oatplanting area of grassland agriculture
Also increasing considerably, therefore the demand of high-quality oat seed significantly rises.Elicitation technique improves seed vitality as one
And the effective ways of seedling growth, to improving, seed Seed practical value is significant.But, cause and be unfavorable for that seed is store
Hide, shorten seed longeivity, constrain its application in seed commodity production and sale, and then forage grass production and animal husbandry are sent out
Exhibition adversely affects.Therefore, it is used as to strengthen the shortage of the high temperature resistant storage technique of Herba bromi japonici Priming Seeds, research for melatonin
And determine that melatonin strengthens Water Content Conditions and the operation sequence of the high temperature resistant storage of Herba bromi japonici Priming Seeds, significant.
Summary of the invention
The invention provides a kind of method utilizing melatonin to strengthen the high temperature resistant storage of Herba bromi japonici Priming Seeds, concrete steps
For:
(1) select for examination oat seed: with normal seedling for criterion calculation percentage of seedgermination, be 100% by germination percentage
High activity level oat seed is as examination oat seed；
(2) adjustment of seed moisture content: according to seed initial water content and seed aqueous amount adjustment method, at seed safety
In storage water content ranges, 7%, 10% and 13% will be adjusted to for the water content of examination oat seed；
(3) seed priming processes: use 100-300 μM of melatonin to cause for examination oat seed 4-8h；
(4) simulation high-temperature storage processes: use the method simulation seeds high-temperature storage controlling deterioration to process, by different water cut
The Herba bromi japonici Priming Seeds of amount carries out deterioration process in being placed in 45 DEG C of high constant water bath box；
(5) determination of the high temperature resistant holding conditions of melatonin Priming Seeds: according to Herba bromi japonici Priming Seeds at different moisture content bar
The germination percentage after high-temperature storage processes, Seedling length, Miao Chong, seedling vigor index variation it is simulated under part, in conjunction with statistical analysis,
Determine that melatonin strengthens the appropriate aqueous amount condition of the high temperature resistant storage of Herba bromi japonici Priming Seeds.
Preferably, the water content for examination oat seed is 7% or 10%.
When carrying out deterioration process in step (4), water content is the Herba bromi japonici Priming Seeds high temperature respectively of 7%, 10% and 13%
Process 40d, 20d and 12d.
Preferably, the melatonin solution using concentration to be 200 μMs processes 6h to for examination oat seed；Priming Seeds is high temperature resistant
The appropriate aqueous amount of storage is 7% or 10%.
The method have the advantages that
(1) present invention can be as strengthening the high temperature resistant storage performance of Herba bromi japonici Priming Seeds, promoting that seed germination and seedling are raw
Long effective ways.On the one hand, it is 7% that test determines the water content of Herba bromi japonici Priming Seeds safe storage under the high temperature conditions,
It is that change of moisture content a series of in the range of 7%-13% are analyzed and result of the comparison；On the other hand, test determines
Under 10% Water Content Conditions, melatonin can make the resistance to 45 DEG C of high-temperature storages of Herba bromi japonici Priming Seeds extend to 12d, causes Herba bromi japonici kind than water
The son many 4d of high temperature resistant storage time.Therefore, utilize this technology can strengthen the performance of the high temperature resistant storage of Herba bromi japonici Priming Seeds, promote
Priming Seeds is sprouted and growth of seedling.
(2) operation sequence setting up this technology standardization advantageously accounts for what elicitation technique faced in production application
Problem.
Accompanying drawing explanation
Fig. 1 is that melatonin affects schematic diagram to different moisture content Herba bromi japonici Priming Seeds germination percentage；
Fig. 2 is that melatonin affects schematic diagram to different moisture content Herba bromi japonici Priming Seeds seedling Seedling length；
Fig. 3 is that melatonin affects schematic diagram to what different moisture content Herba bromi japonici Priming Seeds seedling Seedling weighed；
Fig. 4 is that melatonin affects schematic diagram to different moisture content Herba bromi japonici Priming Seeds seedling vigor index.
In the accompanying drawings, under the identical deterioration time, different lower cases represent significant difference between C, HP and MP (P ＜ 0.05).
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described further, but protection not thereby limiting the invention
Scope.Method flow
1.1 select for examination oat seed material
For guaranteeing Priming Seeds high temperature resistant storage research and the practicality of application, send out with normal seedling for criterion calculation seed
The method of bud rate judges seed vitality level, and the seed (initial water content 8.9%) selecting germination percentage to be 100% is as examination
Material.
1.2 seed moisture contents measure
With reference to international Seed Inspection association (ISTA, 2015) Seed Inspection code the 9th chapter, measure seed moisture content.Weigh
Clean seed is weighed (being accurate to 0.001g) after about 4.5g puts into sample box, arranges two repetitions.At 130 DEG C-133 DEG C, will
Sample lid puts into drying in oven 2h after opening.After arriving the time of regulation, build sample lid, put into and cool down in exsiccator
It is re-weighed after 30min.Seed moisture content (MC), i.e. seed moisture content (%)=(M is calculated by formula2-M3)/(M2-M1)×
100%.
M1: sample box and the weight (g) of lid；
M2: weight (g) before the baking of sample box and lid and sample；
M3: weight (g) after the baking of sample box and lid and sample.
1.3 seed moisture contents adjust
According to seed initial water content, calculate kind corresponding when seed moisture content is adjusted to 7%, 10% and 13%
Sub-weight.The method using saturated salt solution balance static weighing, puts into oat seed and fills saturated MgCl2, NaBr and KBr
Hermetic container inner equilibrium 7d of saturated solution (equilibrium relative humidity (ERH) is respectively 33%, 57% and 80%), weighs seed weight repeatedly
Amount, until seed reaches the weight corresponding to specified moisture content.
1.4 seed primings process
By oat seed respectively with 15mL distilled water (H2O) and 200 μMs of melatonin solution process under 20 DEG C of dark conditions
6h (is denoted as HP and MP) respectively, seed weight: liquor capacity (w/v)=1:3, not cause process as comparison (being denoted as C).To plant
Son takes out, distilled water flushing 3 times, and filter paper blots the surface of the seed moisture, 20 DEG C, under 33%RH dark condition natural air drying adjust to
Seed moisture content is 7%, 10% and 13%.After reaching specified moisture content, close in seed is loaded immediately 9cm × 13cm aluminium foil bag
Envelope, 200 every bag.
1.5 simulation high-temperature storages process
The method simulation seeds high-temperature storage controlling deterioration is used to process.The Herba bromi japonici Priming Seeds of different moisture content is placed in
Deterioration process is carried out in 45 DEG C of high constant water bath box.7%, the Priming Seeds of 10% and 13% water content respectively high-temperature process 40d,
20d and 12d, and sample once every 8d, 4d and 2d respectively, in case test is used.
1.6 germinations measure
With reference to ISTA (2015) regulation, choose uniform full 50 consistent, seed, put it into and fill 3 metafiltration paper
In 11cm × 11cm Petri dish, filter paper 10mL distilled water moistening, 20 in illumination box (GXZ-380B-LED)
Cultivate under the conditions of DEG C illumination 8h, dark 16h.Using normal seedling as germination standard, calculate sending out after 10d germination terminates
Bud rate.
Germination percentage (G)=(G10/ N) × 100%, wherein G10For front 10d all normal seedling numbers, N is for total for planting experimentally son
Number.
1.7 seedling growths measure
Carry out according to germinating method in 1.6, using normal seedling as germination standard, after 10d germinates and terminates, survey
The most normal fixed root length of seedling, Seedling length and Seedling fresh weight, calculate seedling vigor index (SVI).
Seedling vigor index (SVI)=(ARL+ASL) × G, wherein, ARL be average root length (cm), ASL be that average Seedling is long
(cm)。
1.8 data process and analyze
Test data uses Excel2010 and SPSS17.0 software to carry out significance statistical analysis, uses
SigmaPlot10.0 software is mapped.Result represents with meansigma methods ± standard error.
Embodiment 1
1. select for examination oat seed
The present invention selects the oat seed sample of germination percentage 100%, kind herdsman, within 2014, originates in the U.S., seed lot
Number Y16-14-CC-10.Essential information for examination oat seed sample is shown in Table 1.
Table 1 is for examination oat seed essential information
2. seed moisture content measures and adjusts
With reference to international Seed Inspection association (ISTA, 2015) Seed Inspection code the 9th chapter regulation, measure seed moisture content
(MC)。
According to the initial water content of seed, calculate when seed moisture content is adjusted to 7%, 10% and 13% corresponding
Seed weight.The method using saturated salt solution balance static weighing, puts into oat seed and fills saturated MgCl2, NaBr and
Hermetic container inner equilibrium 7d of KBr saturated solution (equilibrium relative humidity (ERH) is respectively 33%, 57% and 80%), weighs seed repeatedly
Weight, until seed reaches the weight corresponding to specified moisture content.
3. seed priming processes
By oat seed respectively with 15mL distilled water (H2O) and 200 μMs of melatonin solution process under 20 DEG C of dark conditions
6h, seed weight: liquor capacity (w/v)=1:3, not cause process as comparison.Seed is taken out, distilled water flushing 3 times, filter
Paper blots the surface of the seed moisture, 20 DEG C, under 33%RH dark condition natural air drying adjust to seed moisture content be 7%, 10% and
13%.After reaching specified moisture content, seal in seed is loaded immediately 9cm × 13cm aluminium foil bag, 200 every bag.
4. simulation high-temperature storage processes
Deterioration process is carried out in the Herba bromi japonici Priming Seeds of different moisture content is placed in 45 DEG C of high constant water bath box.7%, 10%
Distinguish high-temperature process 40d, 20d and 12d with the Priming Seeds of 13% water content, and sample once every 8d, 4d and 2d respectively, with
Standby test is used.
5. different moisture content Herba bromi japonici Priming Seeds is sprouted and the impact of growth of seedling by melatonin
5.1 the impact that different moisture content Herba bromi japonici Priming Seeds is sprouted by melatonin
7%MC oat seed during 40d high temperature deterioration, the germination percentage of HP and MP Priming Seeds with compare C phase ratio
Different not notable (P ＞ 0.05), is held in 100% (Fig. 1).
10%MC oat seed is during 20d high temperature deterioration, and comparison C percentage of seedgermination is always held at 100%, and HP
All reduce with the germination percentage of MP Priming Seeds, and all drop to less than 5% when deterioration 20d；When high temperature deterioration 4d and 8d, HP causes
The germination percentage of seed notable (P ＜ 0.05) is less than comparison C, respectively 96% and 77%, and the germination percentage of MP Priming Seeds is with right
Difference is compared not notable (P ＞ 0.05), respectively 99% and 94%, and the germination percentage of MP Priming Seeds during high temperature deterioration 8d according to C
Significantly (P ＜ 0.05) is higher than the germination percentage of HP Priming Seeds；When high temperature deterioration 12d and 16d, the germination percentage of HP and MP Priming Seeds
All notable (P ＜ 0.05) is less than comparison C, and the germination percentage (respectively 70% and 25%) of MP Priming Seeds is notable (P ＜ 0.05)
Germination percentage (respectively 30% and 10%) higher than HP Priming Seeds.
After 13%MC oat seed high temperature deterioration 8d, comparison C percentage of seedgermination is reduced to 66% by 100%, and HP and MP
The germination percentage of Priming Seeds all reduces to 0%；During high temperature deterioration 2d, the germination percentage of HP with MP Priming Seeds is inequality compared with comparison C
Different not notable (P ＞ 0.05), respectively 90% and 96%；During high temperature deterioration 4d-8d, the germination percentage of HP and MP Priming Seeds is the most aobvious
Write (P ＜ 0.05) less than compareing C, and during deterioration 4d, the germination percentage difference of HP and MP Priming Seeds is notable (P ＞ 0.05), and
During deterioration 6d, the germination percentage of MP Priming Seeds all notable (P ＜ 0.05) is higher than the germination percentage (Fig. 1) of HP Priming Seeds.It is visible,
10%MC seed is during 8d-16d high temperature deterioration, and compared with water initiation HP, melatonin causes MP to be conducive to strengthening seed pair
The toleration of high temperature, slows down the reduction of percentage of seedgermination.
The impact on the seedling Seedling length of different moisture content Herba bromi japonici Priming Seeds of 5.2 melatonin
7%MC oat seed is during 40d high temperature deterioration, and the seedling Seedling length of comparison C, HP and MP Priming Seeds is the most
Reduce, dropped to 10.70cm, 10.57cm and 10.90cm (deterioration by 11.53cm, 11.60cm and 11.55cm (deterioration 0d) respectively
40d), but the seedling Seedling length of HP with MP Priming Seeds equal difference compared with comparison C is notable (P ＞ 0.05) (Fig. 2).
10%MC oat seed is during 20d high temperature deterioration, and the seedling Seedling length of comparison C seed is dropped to by 11.80cm
10.66cm, and the seedling Seedling length of HP and MP Priming Seeds is dropped to 3.41cm and 0.81cm by 10.75cm and 10.84cm respectively,
And the seedling Seedling length of 20d high temperature deterioration process HP and MP Priming Seeds all notable (P ＜ 0.05) is less than comparison C；High temperature deterioration 4d
During with 8d, the seedling Seedling length of MP Priming Seeds notable (P ＜ 0.05) is long higher than the seedling Seedling of HP Priming Seeds；Deterioration 12d and 16d
Time, between the seedling Seedling length of HP and MP Priming Seeds, difference is not notable (P ＞ 0.05).
After 13%MC oat seed high temperature deterioration 8d, the seedling Seedling length of comparison C seed is reduced to 8.49cm by 11.58cm,
And the seedling Seedling length of HP and MP Priming Seeds all reduces to 0cm (Fig. 2)；When high temperature deterioration 4d and 6d, the seedling Seedling of MP Priming Seeds
Long notable (P ＜ 0.05) is long higher than the seedling Seedling of HP Priming Seeds.
The impact on the seedling Seedling weight of different moisture content Herba bromi japonici Priming Seeds of 5.3 melatonin
7%MC oat seed is during 40d high temperature deterioration, and the seedling Seedling weight average of comparison C, HP and MP Priming Seeds is
Decline, reduce 0.388g, 0.314g and 0.188g (Fig. 3) respectively.
10%MC oat seed is during 20d high temperature deterioration, and the seedling Seedling of comparison C, HP and MP Priming Seeds is heavily distinguished
Reduce 0.180g, 3.617g and 3.750g, during deterioration 4d, not notable (the P ＞ of difference between the seedling Seedling weight of HP and MP Priming Seeds
0.05), and during high temperature deterioration 8d-16d, the seedling Seedling representation of MP Priming Seeds writes (P ＜ 0.05) children higher than HP Priming Seeds
Miao Miaochong.
13%MC oat seed is during 8d high temperature deterioration, and the seedling Seedling of comparison C, HP and MP Priming Seeds drops the most respectively
Low 2.259g, 4.526g and 4.434g, during deterioration 2d, notable (the P ＞ of difference between the seedling Seedling weight of HP and MP Priming Seeds
0.05), and during high temperature deterioration 4d-6d, the seedling Seedling representation of MP Priming Seeds writes (P ＜ 0.05) seedling higher than HP Priming Seeds
Miao Chong.
The impact on the seedling vigor index of different moisture content Herba bromi japonici Priming Seeds of 5.4 melatonin
7%MC oat seed is during 40d high temperature deterioration, and the seedling vigor index of comparison C, HP and MP Priming Seeds is equal
Decline, reduced 167.075,147.565 and 63.715 (Fig. 4) respectively, the children of MP Priming Seeds when roguing becomes 24d-32d
Outside Seedling vitality index notable (P ＜ 0.05) is higher than the seedling vigor index of HP Priming Seeds, during other deterioration times, HP and MP draws
Send out the seedling vigor index of seed all with compare C difference significantly (P ＞ 0.05).
10%MC oat seed, during 20d high temperature deterioration, compares the seedling vigor index of C, HP and MP Priming Seeds
All decline, reduce 302.600,1941.900 and 1949.500 (Fig. 4) respectively；During deterioration 4d-16d, HP and MP causes
The seedling vigor index of seed all notable (P ＜ 0.05) is less than the seedling vigor index of comparison C seed, but the children of MP Priming Seeds
Seedling vitality index notable (P ＜ 0.05) is higher than the seedling vigor index of HP Priming Seeds.
13%MC oat seed is during 8d high temperature deterioration, and the seedling vigor index of comparison C, HP and MP Priming Seeds is equal
Decline, reduce 1006.190,2119.540 and 2057.750 (Fig. 4) respectively；The seedling vigor of MP Priming Seeds during deterioration 4d
Index notable (P ＜ 0.05) is higher than the seedling vigor index of HP Priming Seeds.
In sum, under the conditions of 7%MC, HP and MP Priming Seeds is all resistant to high-temperature storage 40d, and germination percentage all can keep
100%；Under the conditions of 10%MC, HP Priming Seeds is resistant to high-temperature storage 8d, and germination percentage is 77%, and MP Priming Seeds is resistant to height
Temperature storage 12d, germination percentage is 70%, and simultaneously during 8d-12d high temperature deterioration, compared with water initiation HP, melatonin causes MP to have
It is beneficial to strengthen the seed toleration to high temperature, slows down the reduction of percentage of seedgermination；Under the conditions of 13%MC, HP and MP Priming Seeds is equal
Being resistant to high-temperature storage 4d, germination percentage is respectively 64% and 72%.Therefore, in seed safety storage water content ranges, cause and plant
The water content of son is the lowest, and Priming Seeds storage time under the high temperature conditions is the longest；Compared with 10%MC and 13%MC, 7%MC
It is more beneficial for the high-temperature storage of Herba bromi japonici Priming Seeds.Although additionally, cause the storage being unfavorable for seed, seed longeivity can be reduced,
But compared with causing with water, melatonin causes the high temperature resistant Storage being more beneficial for strengthening seed.
Claims (5)
1. one kind utilizes the method that melatonin strengthens the high temperature resistant storage of Herba bromi japonici Priming Seeds, it is characterised in that use 100-300 μM
Melatonin causes for examination oat seed 4-8h.
Method the most according to claim 1, it is characterised in that concretely comprise the following steps:
(1) select for examination oat seed: with normal seedling for criterion calculation percentage of seedgermination, the height that germination percentage is 100% is lived
Force level oat seed is as examination oat seed；
(2) adjustment of seed moisture content: 7%, 10% and 13% will be adjusted to for the water content of examination oat seed；
(3) seed priming processes: use 100-300 μM of melatonin to cause for examination oat seed 4-8h；
(4) simulation high-temperature storage processes: carry out in the Herba bromi japonici Priming Seeds of different moisture content is placed in 45 DEG C of high constant water bath box
Deterioration processes；
(5) determination of the high temperature resistant holding conditions of melatonin Priming Seeds: according to Herba bromi japonici Priming Seeds under the conditions of different moisture content
It is simulated the germination percentage after high-temperature storage processes, Seedling length, Miao Chong, seedling vigor index variation, in conjunction with statistical analysis, determines
Melatonin strengthens the appropriate aqueous amount condition of the high temperature resistant storage of Herba bromi japonici Priming Seeds.
Method the most according to claim 2, it is characterised in that the water content for examination oat seed is 7% or 10%.
Method the most according to claim 2, it is characterised in that carry out deterioration in step (4) when processing, water content is 7%,
The Herba bromi japonici Priming Seeds of 10% and 13% high-temperature process 40d, 20d and 12d respectively.
Method the most according to claim 2, it is characterised in that the melatonin solution using concentration to be 200 μMs is to for examination swallow
Wheat Seeds processes 6h；The appropriate aqueous amount of storage that Priming Seeds is high temperature resistant is 7% or 10%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610453578.1A CN106068779B (en) | 2016-06-21 | 2016-06-21 | A method of causing seed high temperature resistant using epiphysin enhancing oat and stores |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610453578.1A CN106068779B (en) | 2016-06-21 | 2016-06-21 | A method of causing seed high temperature resistant using epiphysin enhancing oat and stores |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106068779A true CN106068779A (en) | 2016-11-09 |
| CN106068779B CN106068779B (en) | 2019-06-04 |
Family
ID=57238783
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610453578.1A Expired - Fee Related CN106068779B (en) | 2016-06-21 | 2016-06-21 | A method of causing seed high temperature resistant using epiphysin enhancing oat and stores |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106068779B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110679410A (en) * | 2019-11-13 | 2020-01-14 | 上海市农业科学院 | Treatment method for improving heat resistance of lettuce |
| CN111869366A (en) * | 2020-08-18 | 2020-11-03 | 罗杰 | Method for priming tobacco seeds for extended storage time |
| CN111903844A (en) * | 2020-08-07 | 2020-11-10 | 中国热带农业科学院热带作物品种资源研究所 | Method for improving fermentation quality of silage and prepared feed |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104350836A (en) * | 2014-11-11 | 2015-02-18 | 黑龙江八一农垦大学 | New application of melatonin to improving saline-alkaline stress resistance of plant seeds |
| CN104412979A (en) * | 2013-08-19 | 2015-03-18 | 中国科学院遗传与发育生物学研究所 | New applications of melatonin |
-
2016
- 2016-06-21 CN CN201610453578.1A patent/CN106068779B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104412979A (en) * | 2013-08-19 | 2015-03-18 | 中国科学院遗传与发育生物学研究所 | New applications of melatonin |
| CN104350836A (en) * | 2014-11-11 | 2015-02-18 | 黑龙江八一农垦大学 | New application of melatonin to improving saline-alkaline stress resistance of plant seeds |
Non-Patent Citations (2)
| Title |
|---|
| 姜超强 祖朝龙: "褪黑素与植物抗逆性研究进展", 《生物技术通报》 * |
| 赵燕等: "植物中褪黑素的研究进展", 《西北植物学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110679410A (en) * | 2019-11-13 | 2020-01-14 | 上海市农业科学院 | Treatment method for improving heat resistance of lettuce |
| CN111903844A (en) * | 2020-08-07 | 2020-11-10 | 中国热带农业科学院热带作物品种资源研究所 | Method for improving fermentation quality of silage and prepared feed |
| CN111903844B (en) * | 2020-08-07 | 2022-05-13 | 中国热带农业科学院热带作物品种资源研究所 | Method for improving fermentation quality of silage and prepared feed |
| CN111869366A (en) * | 2020-08-18 | 2020-11-03 | 罗杰 | Method for priming tobacco seeds for extended storage time |
| CN111869366B (en) * | 2020-08-18 | 2021-10-26 | 黔西南州烟草公司兴义市分公司 | Method for priming tobacco seeds for extended storage time |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106068779B (en) | 2019-06-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106034463B (en) | A method of promoting the sprouting of oat Aged Seeds and growth of seedling using epiphysin | |
| May et al. | Pre-sowing hardening of plants to drought: an appraisal of the contributions by PA Genkel. | |
| CN104350834B (en) | Improve the soaking processing method of corn seed top soil germination rate | |
| CN106068779A (en) | A kind of method utilizing melatonin to strengthen the high temperature resistant storage of Herba bromi japonici Priming Seeds | |
| CN108966729A (en) | A kind of characterization and evaluation method of Maize at Seedling Stage salt tolerance | |
| Grabe | Glutamic acid decarboxylase activity as a measure of seedling vigor | |
| Uchendu et al. | Cryopreservation of potato microtubers: the critical roles of sucrose and desiccation | |
| Wahyono et al. | Low irradiation dose for sorghum seed sterilization: hydroponic fodder system and in vitro study | |
| Kvaalen et al. | Effects of various partial pressures of oxygen and carbon dioxide on different stages of somatic embryogenesis in Picea abies | |
| Smiderle et al. | Physiological quality of cowpea seeds for different periods of storage1 | |
| Nezamdoost et al. | Can seed priming with seaweed extract neutralize the effects of salinity on new red fire leafy lettuce characteristics? | |
| Radian et al. | Nanotechnology for dryland agriculture water saving: Biodegradable hydrogel application in sweet corn (Zea mays saccharate Sturt) productio | |
| CN106171123A (en) | Gamma aminobutyric acid improves maize under NaCl stress seed germination and the purposes of root growth | |
| Powell et al. | The Effect of Controlled Limited Moisture on Seedling Growth of Cheyenne Winter Wheat Selections 1 | |
| CN106717304A (en) | A kind of rice paddy seed sterilization method | |
| CN106804153B (en) | Application method of different exogenous regulating substances to corn seed germination and seedling alleviation under low-temperature stress | |
| CN110200216A (en) | A kind of production method of high gamma-aminobutyric acid sprouted unpolished rice gruel can | |
| CN109168689A (en) | The big tree tea Camless valve train matrix in Nanchuan and its cottage method | |
| CN107568165A (en) | A kind of experimental method evaluated genetically modified plants and influenceed on snail | |
| Samarah | Effect of air‐drying immature seeds in harvested pods on seed quality of common vetch (Vicia sativa L.) | |
| Fanous | Test for Drought Resistance in Pearl Millet (Pennisetum typhoideum) 1 | |
| CN104067923B (en) | The breeding method of a kind of high protein, homovitamin C content radish seed bud dish | |
| Oliveira et al. | Morphology of fruits and seeds and germinate and initial development analysis of Hancornia speciosa | |
| Sanyal et al. | Effect of post-anthesis high temperature on seed longevity and vigour in wheat (Triticum aestivum L.). | |
| Jadhav et al. | SEED GERMINATION AND VIGOR |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190604 Termination date: 20200621 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |