A kind of acer truncatum cultivation and grow method of using 5-ALA
Technical field
The present invention relates to the cultivation method of a kind of acer truncatum, relate to specifically the acer truncatum cultivation and grow method of a kind of 5-ALA of using.
Background technology
Acer truncatum (Aacer truncatum Bunge), for Aceraceae maple belongs to deciduous tree, has another name called acer truncatum, because of samara shape, as ancient Chinese silver ingot " ingot ", gains the name, and be Chinese endemic tree, there is drought resisting, the characteristic that barren-resistant, vitality is strong.These seeds are to integrate high-quality oil plant, tanning material, vegetable protein, industrial chemicals, medicinal health and ecological protection, the special precious economic tree with several functions such as timber, red autumnal leaves Characteristic landscapes.China's acer truncatum cultivating and growing and production application technology etc. are also in the starting stage, generally: (1) graft technology: to adopt 1 year life stubble seedling to carry out the effective way that plate budding is the good acer truncatum seeds of Fast-propagation and promotion precocity fruiting at the beginning of the 7--8 month or at the beginning of 9 months.(2) introducing a fine variety while growing seedlings, acer truncatum hibernaculum is undeveloped, and Ce Ya Meng Bi power is strong, and it is vital in seedling raising process, protecting terminal bud.(3) height of seedling of acer truncatum nursery stock transplanting rests in 3--5 centimetre, can Arbuscular Mycorrhizal Fungi bacterium, and as inoculation Pt bacterium, every cave 1ml, effect is remarkable, and before afforestation, reply root system is repaired, and can adopt whole ground, large cave, root system is unfolded, favourable raising survival rate.Afforestation seeding due to various places gathers acer truncatum seedling randomness by force at present, between self-sow and tame individual plant, differs greatly, and seed quality mixes, uneven, directly affects the production application of acer truncatum.
Known at present, 5-ALA (5-ALA) is 4 azole compounds such as porphyrin, chlorophyll, the cobalamin important intermediate of conversion mutually, is that natural existence in plant corpus, plant vital activity are essential, the active physiological activator of metabolism.It is weed killer herbicide, insecticide, antimicrobial agents, plant growth promoter etc. as a kind of non-harmful green, has been subject to the extensive concern of domestic and international researcher and industrial circle.Research shows, the 5-ALA of high concentration presents the effect of weed killer herbicide, and the 5-ALA of low concentration can improve the abilities such as plant salt tolerance alkali, drought resisting, anti-low temperature and poor light, strengthens resistance, and Promoting plant growth improves output, improves fruit quality.
Recent study shows, the effect that 5-ALA has comprises the stability that regulates chlorophyll to synthesize, improve chlorophyll and catch lightsystemⅡ, improves photosynthetic efficiency, affect respiration, promotes to transport and otherwise effect in plant tissue differentiation, organism.Wherein, regulate chlorophyll synthetic aspect: it is generally acknowledged that 5-ALA is the essential precursor substance of chlcrophyll biosynthesis, and can also regulate chlorophyllous synthesizing.In higher plant chloroplast, the 5-ALA that can synthesize protochlorophyllide (is called 5-ALA
h) may adhere to different 5-ALA storehouses separately, building-up process is also by different mechanism regulatings.No matter from external source, supply with experiment, be that root system soaks or foliage-spray, can be to the whole physiological effect that produces of plant.Aspect improving chlorophyll and catching the stability of lightsystemⅡ: tracer analysis announcement, 5-ALA processes the sufficient chlorophyll a of induction generation and is conducive to change into chlorophyll b, the combination of the latter LHC II apoprotein, can avoid the impact of hydrolase and play the effect of stabilize proteins structure.Acting body is in other respects present: there are some researches show after 5-ALA processes and can expand the pore of plant, and improve CO
2crystallized ability.The physiologically active of 5-ALA is subject to the impact of the environmental factors such as light, nutrient source, temperature, and the same with other plant growth regulator, and due to processing method, concentration for the treatment of and processing time difference, effect is different.Therefore, in actual production, must be noted.
Root system is the important component part of plant, and it is the result that overground part photosynthesis and underground part root mass absorb moisture, nutrient coordination that plant normal growth is grown.Root morphology determines that plant obtains the ability of moisture and nutrient, and the root length of reflection Root morphology index, root system surface area, root system volume, root biomass etc. are to affect the vitals that output forms.Therefore, root system of plant research is the important content of ecological study, studies have shown that in a large number, the quality of root growth, directly restricting the growth of acrial part, and the drought resisting mechanism of plant is had to larger impact, especially in arid and semi-arid area, cultivating good root system is one of key measures improving forest survival rate and amount of growth.The growth of root system of plant is for moisture, nutrient and store the synthetic of crucial importance of carbohydrate and growth regulator.In order to guarantee to obtain to greatest extent water source under Drought Stress, crop must be optimized the root structure of self, in form, is mainly that the total root of increase is long, and in spatial distribution, being mainly increases thin length and quantity with (root of d≤2mm is long).
Not enough to the correlative study of acer truncatum for present stage, the present invention aims to provide in a kind of cultivating and growing process that 5-ALA is applied to acer truncatum, obtains the technical method of suitable zones of different acer truncatum Efficient Cultivation plantation.
Summary of the invention
The object of the invention is to overcome the shortcoming and deficiency that in prior art, exist, the acer truncatum cultivation and grow method of a kind of 5-ALA of using is provided.In the present invention, by processing acer truncatum nursery stock and dip in root and process by the 5-ALA with suitable concentration in the cultivating and growing process acer truncatum, strengthen acer truncatum Resistance of Seedling and improved transplanting survival rate.
Object of the present invention is achieved through the following technical solutions: the acer truncatum cultivation and grow method of a kind of 5-ALA of using, comprises the following steps:
1) the 5-ALA solution of preparation 10~40mg/L;
2) get the earth in plantation field, cross 8~10 mesh sieves;
3) by step 1) the 5-ALA solution and step 2 of 10~40mg/L of preparation) the earth hybrid modulation prepared becomes mud standby, and described 5-ALA solution is 200~300mL:1kg with the volume mass ratio of earth;
4) bareroot seedling is dipped in step 3) transplant after dipping in root after the mud that modulates;
5) until step 4) transplant seedling and conform for 10~20 days after, carrying out leaf surface spraying concentration is the 5-ALA solution of 30mg/L~60mg/L, cultivates and obtains healthy and strong acer truncatum seedling.
The acer truncatum seedling obtaining through cultivation and grow method of the present invention, system general length and the thin length of following all have raising in various degree, have optimized the root structure of acer truncatum seeds nursery stocks, to improving drought-resistant ability, have positive role.Acer truncatum seedling after simultaneously transplanting all increases to some extent in parameters such as leaf weight, leaf area, height of seedling, leading thread, ground biomass.
The solvent of the 5-ALA solution step 1) is water;
Step 2) object of sieving described in is to remove the bulk impurity such as stone etc.;
Step 4) bareroot seedling described in is preferably the acer truncatum seedling that plant height is 10~40cm;
Step 4) transplanting described in is preferably density at 90~110cm*90~110cm, makes its density meet the requirement of directive breeding seedlings; After transplanting time fall foliage, to feud or after spring break, before germinateing, all can carry out.
Conforming as having young leaves or sprouting to grow step 5).
The present invention has following advantage and effect with respect to prior art:
The present invention processes acer truncatum seedling with the 5-ALA of low concentration, to strengthen acer truncatum Resistance of Seedling, improves transplanting survival rate; With 5-ALA, seedling is sprayed, improved growth of seedling quality.The acer truncatum seedling obtaining through cultivation and grow method of the present invention, system general length and the thin length of following all have raising in various degree, have optimized the root structure of acer truncatum seeds nursery stocks, to improving drought-resistant ability, have positive role.Acer truncatum seedling after simultaneously transplanting all increases to some extent in parameters such as leaf weight, leaf area, height of seedling, leading thread, ground biomass.After the mud that the present invention modulates on bareroot seedling is dipped in, transplant and dip in after root, then carry out foliage-spray, transplanting survival rate is obviously improved, survival rate is more than 91%; Well developed root system, resistance, is applicable to the acer truncatum cultivating and growing of China's regional.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to this.
Embodiment 1
1) the 5-ALA solution of preparation 10mg/L;
2) get the earth in plantation field, cross 8 mesh sieves and remove the bulk impurity such as stone etc.;
3) by step 1) the 5-ALA solution and step 2 of 10mg/L of preparation) the earth hybrid modulation prepared becomes mud standby; Described 5-ALA solution is 200mL:1kg with the volume mass of earth ratio;
4) bareroot seedling is dipped in step 3) to transplant after dipping in root after the mud that modulates, Transplanting Density is 90cm*90cm;
5) until step 4) transplant 10~20 days seedling and grow after sprouting or young leaves, carrying out leaf surface spraying concentration is the 5-ALA solution of 30mg/L, cultivates and obtains healthy and strong acer truncatum seedling.
Step 4) bareroot seedling described in is preferably the acer truncatum seedling of plant height 10~40cm;
The survival rate of the acer truncatum seedling obtaining through above-mentioned cultivation and grow method is 91%.
Embodiment 2
1) the 5-ALA solution of preparation 30mg/L;
2) get the earth in plantation field, cross the bulk impurity such as 9 mesh sieve de-stone pieces etc.;
3) by step 1) the 5-ALA solution and step 2 of the 30mg/L for preparing) the earth hybrid modulation prepared becomes mud standby; Described 5-ALA solution is 300mL:1kg with the volume mass of earth ratio;
4) bareroot seedling is dipped in step 3) to transplant after dipping in root after the mud that modulates, Transplanting Density is 100cm*100cm;
5) until step 4) transplant 10~20 days seedling and grow after sprouting or young leaves, carrying out leaf surface spraying concentration is the 5-ALA solution of 50mg/L, cultivates and obtains healthy and strong acer truncatum seedling.
Step 4) bareroot seedling described in is preferably the acer truncatum seedling of plant height 10~40cm;
The survival rate of the acer truncatum seedling obtaining through above-mentioned cultivation and grow method is 94%.
Embodiment 3
1) the 5-ALA solution of preparation 40mg/L;
2) get the earth in plantation field, cross the bulk impurity such as 10 mesh sieve de-stone pieces etc.;
3) by step 1) the 5-ALA solution and step 2 of the 40mg/L for preparing) the earth hybrid modulation prepared becomes mud standby; Described 5-ALA solution is 300mL:1kg with the volume mass of earth ratio;
4) bareroot seedling is dipped in step 3) to transplant after dipping in root after the mud that modulates, Transplanting Density is 110cm*110cm;
5) until step 4) transplant 10~20 days seedling and grow after sprouting or young leaves, carrying out leaf surface spraying concentration is the 5-ALA solution of 60mg/L, cultivates and obtains healthy and strong acer truncatum seedling.
Step 4) bareroot seedling described in is preferably the acer truncatum seedling of plant height 10~40cm;
The survival rate of the acer truncatum seedling obtaining through above-mentioned cultivation and grow method is 93%.
Comparative example 1
1) get the earth in plantation field, cross the bulk impurity such as 9 mesh sieve de-stone pieces etc.;
2) by water and step 2) the earth hybrid modulation prepared becomes mud standby; Described water is 300mL:1kg with the volume mass of earth ratio;
3) bareroot seedling is dipped in step 2) to transplant after dipping in root after the mud that modulates, Transplanting Density is 100cm*100cm;
4) until step 3) transplant 10~20 days seedling and grow after sprouting or young leaves, cultivate the acer truncatum seedling obtaining.
Step 3) bareroot seedling described in is preferably the acer truncatum seedling of plant height 10~40cm;
The survival rate of the acer truncatum seedling obtaining through above-mentioned cultivation and grow method is 70%.
Embodiment 3 compares with comparative example 1, and the survival rate of the acer truncatum seedling after transplanting has improved 24%, has significant effect.The acer truncatum that embodiment 1~3 obtains is simultaneously transplanted seedling and compares with comparative example 1, and system general length all has in various degree and improves with length with thin, has optimized the root structure of acer truncatum seeds nursery stocks, to improving drought-resistant ability, has positive role.Acer truncatum seedling after simultaneously transplanting also all increases to some extent in parameters such as leaf weight, leaf area, height of seedling, leading thread, ground biomass.After the mud that the present invention modulates on bareroot seedling is dipped in, transplant and dip in after root, then carry out foliage-spray, transplanting survival rate is obviously improved, survival rate is more than 91%; Well developed root system, resistance, is applicable to the acer truncatum cultivating and growing of China's regional.
Above-described embodiment is preferably embodiment of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under Spirit Essence of the present invention and principle, substitutes, combination, simplify; all should be equivalent substitute mode, within being included in protection scope of the present invention.