CN106069693B - A kind of compound adjusting control agent is alleviating the application in rape salt damage stress - Google Patents
A kind of compound adjusting control agent is alleviating the application in rape salt damage stress Download PDFInfo
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- CN106069693B CN106069693B CN201610525765.6A CN201610525765A CN106069693B CN 106069693 B CN106069693 B CN 106069693B CN 201610525765 A CN201610525765 A CN 201610525765A CN 106069693 B CN106069693 B CN 106069693B
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- Prior art keywords
- epiphysin
- sodium chloride
- brassinosteroid
- rape
- processing
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
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- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Botany (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses the compound adjusting control agents of a kind of epiphysin and brassinosteroid to alleviate the application in rape salt damage stress, step is: a, test process: enclosing one layer of nylon wire in rectangular plastic magazine, it is tightened with rubber band, is placed in the hydroponic box equipped with 1/4 nutrient solution, is arranged on nylon wire, it is placed in culturing room, brightness culture, after select the consistent Brassica campestris L seedling of growing way, be caught in plastic plate with holes, root is immersed in culture solution and is cultivated, it is long to true leaf to Brassica Napus Seedling；B, the measurement of physical signs: after processing, plant height, leaf area, aerial part and the under ground portion fresh weight of rapeseed plants are measured respectively, then finishes, its dry weight is claimed after drying to constant weight；C, data processing；D, the application of the compound formulation of epiphysin and brassinosteroid.The application promotes the growth of rapeseed plants and the accumulation (ground dry weight, underground dry weight) of dry matter, and simple and easy, easy to operate, nontoxic, stability is good.
Description
Technical field
The invention belongs to agriculture plant production fields, are more particularly to a plant growth regulators and are alleviating rape salt
Evil stress in application, the various physiology courses of wide participation plant, can hestening rooting, promote growth, mention seedling, strong sprout, keep a full stand of seedings,
Improving quality, improve yield, especially plant growth and development and its to adverse circumstance in terms of have important adjustment effect.
The damage that this research takes the compound formulation for applying epiphysin and brassinosteroid to alleviate salt stress to rape assigns rape and supports
The ability of anti-poor environment.
Background technique
Crop to the adaptation of adverse circumstance is restricted by two kinds of factors of hereditary capacity and crop body physiological situation, the latter again with
Crop hormone in vivo close relation.Enhance crop using plant growth regulator to the resistivity of adverse circumstance, it has also become
One of the important channel of crop Adverse Resistance Cultivation at present.Salinized soil is a global problem to the threat of agricultural.For a long time,
It is had always been concerned on how to solve the soil salinization to the harm of plant.Rape is as the big oil crops in third place in the world
With the big edible vegetable oil material crop in China first, planting range is very extensive.Saline Land can make plant water suction difficult, wilt
It is lethal, plant nutrient absorption is influenced, so as to cause the underproduction.Therefore the salt tolerance of research rape, the salt resistance ability of raising rape are aobvious
It obtains particularly significant.
Epiphysin has various physiological functions, main includes adjusting as a kind of indole hormone in plant
Photoperiod, removing free radical, protection chlorophyll etc., have been reported and point out, adverse circumstance environment pair can be effectively relieved in epiphysin processing
The depression effect of growth increment, the degradation for slowing down leaf chlorophyll are lost, and are maintained the higher photosynthetic efficiency of plant, are reduced adverse circumstance
Caused by oxidative damage.Brassinosteroid as a kind of new green environment protection plant growth regulator, can hestening rooting, mention
Seedling, strong sprout, volume increase improve its oxidation resistance, to improve the resistance of plant.Epiphysin and brassinosteroid are to adverse circumstance
There is certain relieving effect, but for the also fresh understatement road of research of the compound adjusting control agent of rape salt stress aspect.Present invention application is outer
The compound adjusting control agent of source substance epiphysin and brassinosteroid alleviates salt damage caused by rape to a greater degree, for agricultural
Chemical salt resistance provides the new way of a safe noresidue.
Summary of the invention
The purpose of the invention is to provide the compound adjusting control agents of a kind of epiphysin and brassinosteroid to alleviate rape
Application (promoting plant growth to alleviate the application of damage in rape salt damage stress) in salt damage stress, promotes rape plant
The growth (root long, stem length, ground fresh weight, underground fresh weight) of strain and the accumulation (ground dry weight, underground dry weight) of dry matter, it is simple easy
Row, easy to operate, nontoxic, stability is good.
In order to achieve the above purpose, the present invention uses following technical measures,
A kind of compound adjusting control agent is alleviating the application in rape salt damage stress, the steps include:
1, test process: enclosing one layer of nylon wire in rectangle (22cm х 15cm х 7cm) plastic casing, with rubber band by its
It tightens, is placed in the hydroponic box equipped with 1/4 nutrient solution (52cm х 32cm х 14cm), guarantee that liquid level just touches nylon wire.
Selected full uniform seed, is arranged on nylon wire, is placed in 24-26 DEG C of culturing room, 16/8h brightness culture 6-8d, then
The consistent Brassica campestris L seedling of growing way is selected, is caught in plastic plate with holes, Huo Gelan (Hoagland) culture solution relaying is immersed into root
It is continuous to cultivate, after 4-6d when Brassica Napus Seedling length to 2 true leaves (Fig. 1), under condition of salt stress, carry out following 10 kinds of processing: right
According to (CK1), 0.75% sodium chloride (CK2), 0.75% sodium chloride+30umol/L epiphysin (N+M30), 0.75% sodium chloride+
45umol/L epiphysin (N+M45), 0.75% sodium chloride+60umol/L epiphysin (N+M60), 0.75% sodium chloride+75umol/
L epiphysin (N+M75), 0.75% sodium chloride+0.1umol/L brassinosteroid (N+BR1), 0.75% sodium chloride+0.2umol/L
Brassinosteroid (N+BR2), 0.75% sodium chloride+0.3umol/L brassinosteroid (N+BR3), 0.75% sodium chloride+
0.4umol/L brassinosteroid (N+BR4), 7-10 plants of every processing, 2-4 repetition, 2-4d replace single treatment liquid.
2, the measurement of physical signs: after processing 8-10d, measure respectively the plant heights of 7-10 plants of rapeseed plants, leaf area,
Upper part and under ground portion fresh weight, then in 105-107 DEG C of water-removing 30-45min, 80-85 DEG C claims it dry after drying to constant weight
Weight.
3, data processing: significant difference analysis is carried out to each processing, according to the root long, stem length, overground part of rapeseed plants
The optium concentration 30umol/L and brassinosteroid that point dry weight and fresh weight of plant seedlings and under ground portion dry weight and fresh weight of plant seedlings filter out epiphysin respectively are most
Good concentration 0.2umol/L.
4, the application of the compound formulation of epiphysin and brassinosteroid: under condition of salt stress, the following 4 kinds of processing of setting:
Compare (CK1), 0.75% sodium chloride (CK2), 0.75% sodium chloride+30umol/L epiphysin (N+M30), 0.75% sodium chloride+
0.2umol/L brassinosteroid (N+BR2), 0.75% sodium chloride+30umol/L epiphysin+0.2umol/L brassinosteroid (N+
M30+BR2), it is found that the compound adjusting control agent of 30umol/L epiphysin and 0.2umol/L brassinosteroid coerces tool to rape salt damage
There is significant relaxation effect, promotes the growth of rapeseed plants.
Compared with prior art, the present invention having the following advantages that and effect:
The compound adjusting control agent of epiphysin and brassinosteroid is applied in rape salt damage stress by the present invention, can significantly mitigate
Injury of the salt stress to rapeseed plants, hence it is evident that the ability that rapeseed plants resist salt stress is improved, to reduce caused by salt stress
Yield and quality decline.Compared to single adjusting control agent, effect is more preferable, promote to a greater degree rapeseed plants growth and
The accumulation of dry matter.
Detailed description of the invention
Fig. 1 is growth situation schematic diagram before a kind of processing of rapeseed plants.
Using the method culture rapeseed plants of water planting, it is long to 2 true leaves when handle.
Fig. 2 is that a kind of adjusting control agent alleviates salt damage stress schematic diagram.
It can be seen that from figure, the application of compound adjusting control agent has been relieved salt damage stress, and plant growth condition is substantially better than salt
The plant of harm reason.
Specific embodiment
Present invention is further elaborated in following combination specific embodiment, but the present invention is not limited to following embodiments.
The alleviation application of a kind of compound formulation to growth of rape during embodiment 1:(salt stress)
The compound adjusting control agent of a kind of epiphysin and brassinosteroid is alleviating the application in rape salt damage stress, step
It is:
1, test process: enclosing one layer of nylon wire in rectangle (22cm х 15cm х 7cm) plastic casing, with rubber band by its
It tightens, is placed in the hydroponic box equipped with 1/4 nutrient solution (52cm х 32cm х 14cm), guarantee that liquid level just touches nylon wire.
Selected full uniform seed, is arranged on nylon wire, is placed in 25 DEG C of culturing room, 16/8h brightness culture 6 or 7 or 8 days.So
The same Brassica campestris L seedling of growing way is selected afterwards, is caught in plastic plate with holes, and root is immersed in Huo Gelan (Hoagland) culture solution
Continue to cultivate, after 4 or 5 or 6 days when Brassica Napus Seedling grows 2 true leaves (Fig. 1), under condition of salt stress, carry out 10 kinds following
Processing: control (CK1), 0.75% sodium chloride (CK2), 0.75% sodium chloride+30umol/L epiphysin (N+M30), 0.75% chlorine
Change sodium+45umol/L epiphysin (N+M45), 0.75% sodium chloride+60umol/L epiphysin (N+M60), 0.75% sodium chloride+
75umol/L epiphysin (N+M75), 0.75% sodium chloride+0.1umol/L brassinosteroid (N+BR1), 0.75% sodium chloride+
0.2umol/L brassinosteroid (N+BR2), 0.75% sodium chloride+0.3umol/L brassinosteroid (N+BR3), 0.75% chlorination
Sodium+0.4umol/L brassinosteroid (N+BR4), every 7 or 9 or 10 plants of processing, 2 or 3 or 4 repetitions, replacement one in 2 or 3 or 4 days
Secondary treatment fluid.
2, the measurement of physical signs: after processing 8 or 9 or 10 days, plant height, the leaf of 7 or 9 or 10 plants of rapeseed plants are measured respectively
Area, aerial part and under ground portion fresh weight, then in 105 or 106 or 107 DEG C of water-removing 28 or 29 or 30 or 31 or 32min, 78
Or 79 or 80 or 81 or 82 DEG C claim its dry weight after drying to constant weight.
3, data processing: significant difference analysis is carried out to each processing, according to the root long, stem length, overground part of rapeseed plants
The optium concentration 30umol/L and brassinosteroid that point dry weight and fresh weight of plant seedlings and under ground portion dry weight and fresh weight of plant seedlings filter out epiphysin respectively are most
Good concentration 0.2umol/L.
4, the application of the compound formulation of epiphysin and brassinosteroid:
According to above-mentioned steps 1,2 test process methods, epiphysin, brassinosteroid are weighed respectively, sodium chloride is added to battalion
In nutrient solution, carry out following 5 kinds of processing: control (CK1), 0.75% sodium chloride (CK2), 0.75% sodium chloride+30umol/L are taken off black
Element (N+M30), 0.75% sodium chloride+0.2umol/L brassinosteroid (N+BR2), 0.75% sodium chloride+30umol/L epiphysin
In+0.2umol/L rape element (N+M30+BR2).
When every processing rapeseed plants 7 or 8 or 9 plants, 2 or 3 or 4 repetitions, 2 or 3 or 4 days replacement single treatment liquid.Processing 8
Or after 9 or 10 days, root long, stem length, aerial part and the under ground portion fresh weight of 7 or 8 or 9 plants of rapeseed plants are measured respectively, then
In 105 or 106 or 107 DEG C of water-removing 28 or 29 or 30 or 31 or 32min, 78 or 79 or 80 or 81 or 82 DEG C claim after drying to constant weight
Its dry weight.Acquired results are listed in Table 1 below.
The influence that 1 adjusting control agent of table grows rapeseed plants
Note: different lowercases indicate between different disposals difference up to 5% level of signifiance
As shown in Table 1, rapeseed plants CK2 growing way is weak under salt damage stress, and root long, stem length, ground dry weight and fresh weight of plant seedlings and underground are dry
Weight is substantially less than the control CK1 of normal growth, illustrates that salt stress significantly suppresses the growth of plant, shows apparent salt
Evil symptom.Apply epiphysin M30 and significantly improves the plant height of rape and ground dry weight and fresh weight of plant seedlings under salt stress, root long and underground dry weight and fresh weight of plant seedlings
Though increasing, not up to the level of signifiance, illustrate that epiphysin focuses on its aerial part to the relaxation effect of rape salt stress.
Another aspect applies brassinosteroid BR2 and significantly improves the root long and underground dry weight and fresh weight of plant seedlings of rapeseed plants, plant height and ground dry weight and fresh weight of plant seedlings
It slightly improves, but the not up to level of signifiance, illustrates that epiphysin focuses on its under ground portion to the relaxation effect of rape salt stress.Together
When apply both adjusting control agents compared with applying single adjusting control agent, root long, ground dry weight and fresh weight of plant seedlings and underground dry weight and fresh weight of plant seedlings obtain
It significantly improves, farthest promotes the growth of rapeseed plants.
In conclusion in the application for alleviating rape salt damage stress, the compound adjusting control agent ratio of epiphysin and brassinosteroid
Single adjusting control agent effect is more preferable, promotes the growth of rapeseed plants and the accumulation of dry matter to a greater degree, hence it is evident that improve
Salt damage coerces the upgrowth situation (Fig. 2) of lower rapeseed plants.
Claims (1)
1. the compound adjusting control agent of a kind of epiphysin and brassinosteroid is alleviating the application in rape salt damage stress, the steps include:
A, test process: one layer of nylon wire is enclosed in rectangular plastic magazine, is tightened, is placed in equipped with 1/4 nutrition with rubber band
In the hydroponic box of liquid, liquid level touches nylon wire, selects full seed, is arranged on nylon wire, is placed in 24-26 DEG C of culturing room
In, then 16/8h brightness culture 6-8d selects the consistent Brassica campestris L seedling of growing way, is caught in plastic plate with holes, root is immersed suddenly
Continue to cultivate in Glan culture solution, long to 2 true leaves to Brassica Napus Seedling after 4-6d, under condition of salt stress, progress is 10 kinds following
Processing: control, 0.75% sodium chloride, 0.75% sodium chloride+30umol/L epiphysin, 0.75% sodium chloride+45umol/L are taken off black
Element, 0.75% sodium chloride+60umol/L epiphysin, 0.75% sodium chloride+75umol/L epiphysin, 0.75% sodium chloride+
0.1umol/L brassinosteroid, 0.75% sodium chloride+0.2umol/L brassinosteroid, 0.75% sodium chloride+0.3umol/L oil
Rape lactone, 0.75%NaCl+0.4umol/L brassinosteroid, 7-10 plants of every processing, 2-4 repetition, 2-4d replacement one
Secondary treatment fluid；
B, the measurement of physical signs: after processing 8-10d, plant height, the leaf area, overground part of 7-10 plants of rapeseed plants are measured respectively
Point and under ground portion fresh weight, then in 105-107 DEG C of water-removing 30-45min, 80-85 DEG C claims its dry weight after drying to constant weight；
C, data processing: to each processing carry out variance analysis, according to the root long of rapeseed plants, stem length, aerial part dry weight and fresh weight of plant seedlings and
Under ground portion dry weight and fresh weight of plant seedlings filters out the concentration 30umol/L of the epiphysin and concentration 0.2umol/L of brassinosteroid respectively；
D, the application of the compound formulation of epiphysin and brassinosteroid: under condition of salt stress, the following 4 kinds of processing of setting: control,
0.75% sodium chloride, 0.75% sodium chloride+30umol/L epiphysin, 0.75% sodium chloride+0.2umol/L brassinosteroid,
0.75% sodium chloride+30umol/L epiphysin+0.2umol/L brassinosteroid finds 30umol/L epiphysin and 0.2umol/L
The compound adjusting control agent of brassinosteroid has alleviation to rape salt damage stress, promotes the growth of rapeseed plants.
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| CN107347605A (en) * | 2017-08-03 | 2017-11-17 | 中国农业科学院油料作物研究所 | A kind of application of epiphysin in peanut salt damage stress is alleviated |
| CN109496711A (en) * | 2018-12-29 | 2019-03-22 | 中国农业科学院油料作物研究所 | A kind of serotonin is alleviating the application in rape freeze injury |
| CN111574289A (en) * | 2020-07-01 | 2020-08-25 | 南京彩韵农林科技有限公司 | Special foliar fertilizer for saline-alkali soil and use method thereof |
| CN114868544A (en) * | 2022-04-15 | 2022-08-09 | 江汉大学 | Method for relieving double stress poison of waterlogging and salt of plant seedlings |
| CN116548437B (en) * | 2023-03-30 | 2024-02-20 | 中国热带农业科学院橡胶研究所 | Environment-friendly preparation for preventing and treating anthracnose of rubber tree, preparation method and application thereof |
| CN117296595A (en) * | 2023-10-25 | 2023-12-29 | 玉林师范学院 | Application of 2,4-epibrassinolide in promoting growth of Yulan in salt stress and method for relieving Yulan salt stress toxicity |
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| CN101953337B (en) * | 2010-09-21 | 2013-06-26 | 中国科学院植物研究所 | Plant growth regulator composition and application thereof |
| WO2014079772A1 (en) * | 2012-11-22 | 2014-05-30 | Basf Se | Pesticidal mixtures |
| CN104412979B (en) * | 2013-08-19 | 2016-04-13 | 中国科学院遗传与发育生物学研究所 | The novelty teabag of epiphysin |
| CN103875673B (en) * | 2014-03-24 | 2016-05-11 | 中国农业科学院农业质量标准与检测技术研究所 | The new application of epiphysin in Promoting plant growth |
| CN104350836A (en) * | 2014-11-11 | 2015-02-18 | 黑龙江八一农垦大学 | New application of melatonin to improving saline-alkaline stress resistance of plant seeds |
| CN104604864A (en) * | 2015-02-13 | 2015-05-13 | 中国农业大学 | Melatonin solution capable of improving anti-stress capability of plant |
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