CN106035336A - Complex hormone and preparation method and application thereof - Google Patents
Complex hormone and preparation method and application thereof Download PDFInfo
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- CN106035336A CN106035336A CN201610523130.2A CN201610523130A CN106035336A CN 106035336 A CN106035336 A CN 106035336A CN 201610523130 A CN201610523130 A CN 201610523130A CN 106035336 A CN106035336 A CN 106035336A
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- melatonin
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
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- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a complex hormone and a preparation method and application thereof. The complex hormone is formed by melatonin, salicylic acid and abscisic acid in a certain proportion. The preparation method includes steps: A, using an analytical balance to respectively proportionally weighing the melatonin, the salicylic acid and the abscisic acid at the room temperature; B, well mixing the melatonin, the salicylic acid and the abscisic acid to obtain a mixture; D, adding 1L of full-concentration Hoagland's nutrient solution into the mixture, and well mixing to obtain a complex hormone aqueous solution. The complex hormone is reasonable in formulation, convenient to use, effective in improvement of drought stress resistance of rape, safe and free of pollution to rape products and ecological environment; the method is easy to implement and convenient and simple in operation, and by compounding of different hormones, nutritional disorder caused by action of single hormones is avoided, and crop growth can be promoted comprehensively; under drought stress, lateral root growth is promoted, a flowering time is changed, and leaf senescence is decelerated. In addition, excessive active oxygen generated in rape bodies under drought stress can be cleared to reduce cell membrane permeability and improve drought tolerance of rape, and accordingly yield increase is realized.
Description
Technical field
The present invention relates to agricultural biological technical field, be specifically related to a kind of improve the drought-enduring melatonin coerced of Brassica campestris L
(melatonin, N-acetyl group-5-methoxyl group-tryptamines, C13H16N2O2), salicylic acid (SA, C7H6O3), abscisic acid (ABA,
C15H20O4) composite hormone that forms, also relate to the preparation method of a kind of composite hormone, further relate to the use of a kind of composite hormone
On the way.Growth-regulating hormone plays very important effect in terms of physiological regulation, increase stress resistance of plant, and pouring is taked in this research
Composite hormone alleviates the adverse circumstance infringement to Brassica campestris L, gives the ability of Brassica campestris L opposing poor environment.
Background technology
Crop is to be restricted by inherited character and two kinds of factors of crop body physiological situation the adaptation of adverse circumstance, the latter again with
Crop hormone in vivo close relation.Plant growth regulator can be widely used for coordinate plant growth and improve plant
Anti-adversity ability, therefore explores potential growth regulatory substance and mechanism thereof significant to the drought-resistance ability improving crop.
Arid is a global problem, and the loss caused agricultural because of arid is equivalent to what other bad factor caused
The summation of loss.Brassica campestris L is one of most important oil crop of China, occupies very important in China's oil crop plantation
Status.The Yangtze river basin is China's Brassica campestris L main producing region, although rainfall is abundant, but annual precipitation uneven, seasonal drought is frequent
Occur.Brassica campestris L is the crop that drought tolerance is poor, and the seasonal drought affecting Yangtze river basin Rape-seed production is mainly autumn drought and spring drought,
Its mid-autumn is non-irrigated on Rape-seed production impact maximum.Typically on the occasion of rape seed phase or seedling stage when autumn drought occurs.Rape seedling water consumption
Measure less, but if this phase hydropenia, not only can suppress the growth of its blade and root system, have influence on cultivation and the peace in Brassica campestris L strong sprout
Entirely surviving the winter, seedling plants also can reduce moisture because of rising, and Repiration is aggravated, and synthesizes absorption of nutrient ingredients and photosynthate
Reduce, cause wilting or dead, and centering late growing stage brings a series of negative effect that cannot reverse, and ultimately causes the underproduction.
Correlational study shows the intracellular a large amount of deposition activity oxygen of Under Drought Stress in Plant, causes Lipid peroxidation metabolism free radical chain
Formula is reacted, and causes film to be separated, destroys the normal function of film, and the generation of Peroxidation Product MDA and accumulation can aggravate carefully
The murder by poisoning of born of the same parents.Research is it is also shown that in Brassica Napus Seedling body, MDA content aggregate performance is increasing after PEG6000 process Drought stress simulation
The trend added, shows that plant is caused peroxidative damage by active oxygen.
Abscisic acid, as triggering the carrier that Stress response is reacted by plant, participates in regulation and control plant to environment stress.
Salicylic acid can improve the salt tolerance of plant, drought tolerance, winter resistance and heat resistance etc., the metabolism that salicylic acid is important to some plants
Process plays regulating and controlling effect, and then improves oxidative stress, to promote the disease-resistant and resistance to abiotic stress ability of plant.Melatonin is being planted
Thing has several functions, grows including coordinate plant growth, as promoted the growth of side root, changing florescence, Delaying Leaf-Senescence
Deng.Research at present is thought, melatonin is mainly due to it and participates in the removing of active oxygen the regulation effect of plant stress-resistance.Three it
Between can play one and complement each other, the effect collectively promoted.Plant growth promoter is can to promote to plant under suitable concentration
Thing cell division grows, and improves the yield of plant, but current plant growth promoter is owing to easily decomposing or people is had poison
Evil, need in use to properly protect measure.Application external source composite hormone alleviates the infringement that Brassica campestris L is caused by arid, for chemurgy
Learn drought-enduring water saving and provide the new way of a safe noresidue.
Summary of the invention
It is an object of the invention to there are provided a kind of composite hormone, formula is reasonable, easy to use, can effectively raise
The drought-resistant ability of coercing of Brassica campestris L, and pollution-free to rape product and ecological environment security.
Another object of the present invention is the preparation method that there are provided a kind of composite hormone, easy to implement the method, operation letter
Just, hormon compounds, it is to avoid the nutritional disorder that single hormonal action causes, it is possible to improve the growth of crop comprehensively.Play
Different chemical molecule synergism in improving plant resistance to environment stress induction, enhances the resistance regulation effect to adverse circumstance, to adverse circumstance
Have and preferably defend resistant function.
The present invention's it is also an object that there are provided a kind of composite hormone, Brassica campestris L is drought-resistant coerces improving
Application.In drought stress, this product can promote lateral root growth, changes florescence, Delaying Leaf-Senescence etc..Arid can be removed
The active oxygen of the excess produced in coercing lower Brassica campestris L body, reduces cell leakage, improves the drought tolerance of Brassica campestris L, thus reaches volume increase
Effect.
In order to realize above-mentioned purpose, the present invention uses techniques below measure:
A kind of composite hormone, it is made up of the raw material of following weight portion:
A kind of composite hormone, it is made up (preferably scope) of the raw material of following weight portion:
A kind of composite hormone, it is made up (preferable scope) of the raw material of following weight portion:
A kind of composite hormone, it is made up (best scope) of the raw material of following weight portion:
A kind of composite hormone, it is made up (optimum) of the raw material of following weight portion:
The preparation method of a kind of composite hormone, the steps include:
A, under room temperature (20-25 DEG C, the most identical), weigh melatonin, salicylic acid, de-the most in proportion with analytical balance
Fall acid；
B, melatonin, salicylic acid, abscisic acid are carried out mix homogeneously, it is thus achieved that mixture；
C, in the mixture addition 1L full concentration Glan nutritional solution suddenly, be uniformly mixed so as to obtain heretofore described composite hormone
Aqueous solution.
A kind of composite hormone improve Brassica campestris L is drought-resistant coerce in application, the steps include:
1, choose full uniform Semen Brassicae campestris (MZ08), be positioned over equipped with 1/4 Glan (Hoagland) nutritional solution suddenly
Hydroponic box germinates, opens completely to two panels cotyledon, select seedling of the same size, use water planting, be transplanted to prepare suddenly
Glan (Hoagland) nutritional solution is cultivated；Treat that new plant grows to two panels true leaf, Brassica Napus Seedling is gone to simulating drought inverse
The aqueous solution that border is coerced continues cultivate, the aqueous solution that simulation is coerced adds different Hormone agents.Specifically include following place
Reason: comparison (CK1), 7%PEG process (CK2), 7%PEG+ melatonin, 7%PEG+ salicylic acid, 7%PEG+ abscisic acid, 7%PEG
+ melatonin+salicylic acid+abscisic acid.Each process 22-26 strain rapeseed plants, changes single treatment liquid in 2-4 days.
2, after plant processes 6-8d, the root length of rapeseed plants, stem length, leaf area, aerial parts and underground part are measured respectively
Dividing fresh weight, subsequently in 105 DEG C of 28-32min that complete, 78-82 DEG C of drying claims its dry weight to constant weight.
The present invention compared with prior art, has the following advantages and effect:
Composite hormone of the present invention is the joint product of melatonin+salicylic acid+abscisic acid, applies it to Brassica campestris L and does
During drought is coerced, three complements each other mutually, mutually promotes, and can significantly improve Brassica campestris L Drought resistance.And usage simple and flexible, effect
The most notable, nontoxic, the new way providing a safe noresidue is coerced for Genes For Plant Tolerance.
Accompanying drawing explanation
Fig. 1 is a kind of germination 7d schematic diagram.Two panels cotyledon opens and in the same size completely.
Fig. 2 is that a kind of transplanting is cultivated to two panels true leaf schematic diagram.Long in the same size to two panels true leaf and plant.
Fig. 3 is that under a kind of drought stress, hormon processes 7d schematic diagram.The Brassica campestris L of composite hormone is added under drought stress
Seedling is significantly greater than other Brassica campestris L seedlings processed, and adds taking second place of melatonin, and the Brassica campestris L seedling of simple drought stress is the most weak.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is expanded on further, but the present invention is not limited to following example.
Embodiment 1:
A kind of composite hormone, it is made up of the raw material of following weight portion:
The preparation method of a kind of composite hormone, the steps include:
A, at room temperature weigh melatonin 14 parts, salicylic acid 110.5 parts, abscisic acid 2 parts the most in proportion with analytical balance；
B, the melatonin by weighing, salicylic acid, abscisic acid mix homogeneously, it is thus achieved that mixture；
C, in the mixture addition 1L full concentration Glan nutritional solution suddenly, be uniformly mixed so as to obtain the aqueous solution of composite hormone.A kind of multiple
Closing hormone, it is made up of the raw material of following weight portion:
| Embodiment | 2 | 3 | 4 | 5 | 6 |
| Melatonin | 7 | 16.3 | 9.4 | 15 | 12.8 |
| Salicylic acid | 69 | 124.2 | 89.8 | 110.5 | 138.2 |
| Abscisic acid | 2 | 3.5 | 2.8 | 4 | 4 |
| Amount to | 78 | 144 | 102 | 129.5 | 155 |
Its preparation process is same as in Example 1.
Embodiment 7:
A kind of composite hormone improve Brassica campestris L is drought-resistant coerce in application, the steps include:
A, at hydroponic box (22cm × 15cm × 7cm) attached last layer crocus cloth, tightened with rubber band, be placed in equipped with 1/
In the hydroponic box (52cm × 32cm × 14cm) of 4 Glan (Hoagland) nutritional solutions suddenly, it is ensured that liquid level just touches nylon wire.
Selected full uniform seed (MZ08), is seeded on crocus cloth, is placed in 22 or 23 or 24 or 25 or 26 DEG C of culturing room, 16/
8h light light culture 6 or 7 or 8d.
B, after 6 or 7 or 8 days, select the Brassica campestris L seedling that growing way is the same, be transplanted in plastics field planting plate with holes, root is immersed
Huo Gelan (Hoagland) culture fluid continues grown cultures, in time growing 2 true leaves, carries out following process:
Comparison (CK1, full concentration nutritional solution), 7%PEG process (CK2), 7%PEG+ melatonin aqueous solution, 7%PEG+ water
Poplar aqueous acid, 7%PEG+ abscisic acid aqueous solution, 7%PEG+ composite hormone aqueous solution.Each process 24 strain rapeseed plants, three
It changes single treatment liquid.Process after 6 or 7 or 8d, measure respectively the root length of rapeseed plants, stem length, leaf area, aerial parts and
Under ground portion fresh weight, subsequently in 105 DEG C of complete 28 or 29 or 30 or 31 or 32min, dries extremely for 78 or 79 or 80 or 81 or 82 DEG C
Its dry weight is claimed after constant weight.Acquired results is shown in Table 1.
The impact on rapeseed plants physical signs of table 1 composite hormone
Note: different alphabetic flags represent that between different disposal, difference is in 5% significant level
From table 1, compared with CK comparison, 7%PEG coerces down, the root length of Brassica Napus Seedling, stem length, above and below ground fresh weight,
Dry weight, leaf area all have reduction, illustrate seriously to inhibit under drought stress the growth of Brassica Napus Seedling.Execute in PEG solution respectively
Add SA, ABA, MT coerce with PEG compared with all make various physical signs improve.Illustrate that external source applies hormone and does Brassica campestris L
Drought is coerced has certain regulating and controlling effect.Wherein apply ABA root length, the long increasing value of stem is higher than other two hormones, and ABA master is described
On root to be acted on and plant height；Apply MT and significantly improve fresh dry weight, the fresh dry weight in underground, leaf area on the ground, illustrate that MT is main
Act on aerial parts.Apply SA and mainly act on root, the freshest dry weight and leaf area.SA+ is applied compared with 7%PEG
The composite hormone of MT+ABA composition, is all improved largely to root length, stem length, the freshest dry weight, the fresh dry weight in underground, leaf area,
And various index is all higher than other hormone independent roles.
In sum, although three kinds of single applyings of hormone all have certain regulation effect to drought stress, but effect is all
The most comprehensive.Applying composite hormone makes various physical signs all increase substantially, although illustrating that arid is coerced by SA, ABA and MT
Compel there is alleviation regulating and controlling effect, but effect and mode have must difference, three combination significantly increase Brassica campestris L to drought stress
Resistant function, its degeneration-resistant border effect is more preferable, and usage is simple, and effect is notable, nontoxic, it is adaptable to land for growing field crops is used.
Claims (8)
1. a composite hormone, it is made up of the raw material of following weight portion:
A kind of composite hormone the most according to claim 1, it is characterised in that:
A kind of composite hormone the most according to claim 1, it is characterised in that:
A kind of composite hormone the most according to claim 1, it is characterised in that:
A kind of composite hormone the most according to claim 1, it is characterised in that:
A kind of composite hormone the most according to claim 1, it is characterised in that:
7. the preparation method of a kind of composite hormone described in claim 1, the steps include:
A, at room temperature weigh melatonin, salicylic acid, abscisic acid the most in proportion with analytical balance；
B, melatonin, salicylic acid, abscisic acid are carried out mix homogeneously, it is thus achieved that mixture；
C, in the mixture addition 1L full concentration Glan nutritional solution suddenly, be uniformly mixed so as to obtain the aqueous solution of composite hormone.
8. a kind of composite hormone described in claim 1 improve Brassica campestris L is drought-resistant coerce in application, the steps include:
A, choose full uniform Semen Brassicae campestris, be positioned in the hydroponic box equipped with 1/4 Glan nutritional solution suddenly and germinate, to two slice, thin pieces
Leaf opens completely, selects seedling of the same size, uses water planting, is transplanted in the Huo Gelan nutritional solution prepared cultivate；
Treat that new plant grows to two panels true leaf, go to Brassica Napus Seedling the aqueous solution of simulating drought environment stress continue cultivate, the simulation side of body
Compel aqueous solution in add different Hormone agents, including following process: comparison, 7% Polyethylene Glycol process, 7% Polyethylene Glycol+
Melatonin, 7% Polyethylene Glycol+salicylic acid, 7% Polyethylene Glycol+abscisic acid, 7% Polyethylene Glycol+melatonin+salicylic acid+come off
Acid, each process 22-26 strain rapeseed plants, within 2-4 days, change single treatment liquid；
After B, plant process 6-8d, measure the root length of rapeseed plants, stem length, leaf area, aerial parts and under ground portion respectively fresh
Weight, subsequently in 105 DEG C of 28-32min that complete, 78-82 DEG C of drying claims its dry weight to constant weight.
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| CN106035336B CN106035336B (en) | 2018-11-06 |
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Cited By (9)
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| CN106613702A (en) * | 2016-12-30 | 2017-05-10 | 四川农业大学 | Method for relieving drought stress of Actinidia chinensis fruit trees |
| CN106614738A (en) * | 2016-11-03 | 2017-05-10 | 明光市大全甜叶菊专业合作社 | Seed-soaking fluid for stevia rebaudiana |
| CN107279145A (en) * | 2017-06-20 | 2017-10-24 | 四川国光农化股份有限公司 | A kind of plant growth regualting composition, preparation and its application |
| CN109105374A (en) * | 2018-08-02 | 2019-01-01 | 吉林省农业科学院 | Corn seed initiator and preparation method thereof and the method for promoting corn seed low-temperature resistance to sprout |
| CN111436372A (en) * | 2019-12-23 | 2020-07-24 | 山东农业大学 | Nutrient solution for inducing garlic aerial bulbs, method and application |
| CN112136816A (en) * | 2020-09-27 | 2020-12-29 | 甘肃省科学院生物研究所 | Compound preparation for preventing and treating early bolting of angelica sinensis as well as use method and application thereof |
| CN112154886A (en) * | 2020-10-15 | 2021-01-01 | 云南省烟草农业科学研究院 | Flue-cured tobacco cultivation management method and baking method for relieving drought stress |
| CN112544325A (en) * | 2020-12-08 | 2021-03-26 | 西北农林科技大学 | Method for postponing flowering phase of apples by applying abscisic acid externally |
| CN114560736A (en) * | 2022-02-25 | 2022-05-31 | 浙江省亚热带作物研究所 | Composite nutrition regulator for regulating bougainvillea spectabilis florescence and regulating method thereof |
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Cited By (11)
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| CN106614738A (en) * | 2016-11-03 | 2017-05-10 | 明光市大全甜叶菊专业合作社 | Seed-soaking fluid for stevia rebaudiana |
| CN106613702A (en) * | 2016-12-30 | 2017-05-10 | 四川农业大学 | Method for relieving drought stress of Actinidia chinensis fruit trees |
| CN106613702B (en) * | 2016-12-30 | 2020-07-24 | 四川农业大学 | Method for relieving drought stress of kiwi fruit trees |
| CN107279145A (en) * | 2017-06-20 | 2017-10-24 | 四川国光农化股份有限公司 | A kind of plant growth regualting composition, preparation and its application |
| CN109105374A (en) * | 2018-08-02 | 2019-01-01 | 吉林省农业科学院 | Corn seed initiator and preparation method thereof and the method for promoting corn seed low-temperature resistance to sprout |
| CN109105374B (en) * | 2018-08-02 | 2021-03-23 | 吉林省农业科学院 | Corn seed initiator, preparation method thereof and method for promoting low-temperature germination resistance of corn seeds |
| CN111436372A (en) * | 2019-12-23 | 2020-07-24 | 山东农业大学 | Nutrient solution for inducing garlic aerial bulbs, method and application |
| CN112136816A (en) * | 2020-09-27 | 2020-12-29 | 甘肃省科学院生物研究所 | Compound preparation for preventing and treating early bolting of angelica sinensis as well as use method and application thereof |
| CN112154886A (en) * | 2020-10-15 | 2021-01-01 | 云南省烟草农业科学研究院 | Flue-cured tobacco cultivation management method and baking method for relieving drought stress |
| CN112544325A (en) * | 2020-12-08 | 2021-03-26 | 西北农林科技大学 | Method for postponing flowering phase of apples by applying abscisic acid externally |
| CN114560736A (en) * | 2022-02-25 | 2022-05-31 | 浙江省亚热带作物研究所 | Composite nutrition regulator for regulating bougainvillea spectabilis florescence and regulating method thereof |
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