CN114560736A - Composite nutrition regulator for regulating bougainvillea spectabilis florescence and regulating method thereof - Google Patents
Composite nutrition regulator for regulating bougainvillea spectabilis florescence and regulating method thereof Download PDFInfo
- Publication number
- CN114560736A CN114560736A CN202210182505.9A CN202210182505A CN114560736A CN 114560736 A CN114560736 A CN 114560736A CN 202210182505 A CN202210182505 A CN 202210182505A CN 114560736 A CN114560736 A CN 114560736A
- Authority
- CN
- China
- Prior art keywords
- bougainvillea spectabilis
- flowering
- regulating
- bougainvillea
- spectabilis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Pest Control & Pesticides (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Inorganic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
- Fertilizers (AREA)
Abstract
The invention provides a compound nutrition regulator for regulating and controlling bougainvillea spectabilis florescence and a regulating and controlling method thereof. The application discovers for the first time that under the stress of melatonin and drought, the differentiation time of bougainvillea spectabilis buds can be shortened, the flowering time of the bougainvillea spectabilis is can be obviously advanced, growth hormones such as ethephon, monopotassium phosphate and the like for promoting the differentiation of the bougainvillea spectabilis can be matched, the time required for flowering can be further synergistically shortened, meanwhile, sucrose can promote the synthesis of bougainvillea spectabilis pigment, the composite nutrition regulator is obtained after the components are compounded, and the composite nutrition regulator is used in the regulation and control of the flowering phase of the bougainvillea spectabilis, can obviously shorten the flowering time, prolong the flowering phase time and enhance the color of the bougainvillea spectabilis. The composite nutrient regulator for regulating and controlling the bougainvillea spectabilis florescence and the regulating and controlling method thereof can promote the bougainvillea spectabilis to bloom, shorten the time for blooming, prolong the whole florescence open time and greatly improve the appreciation of the bougainvillea spectabilis.
Description
Technical Field
The invention provides a compound nutrition regulator for regulating and controlling bougainvillea spectabilis florescence and a regulating and controlling method thereof, belonging to the technical field of agriculture.
Background
Bougainvillea spectabilis willld, leaf flower of the same name, Mirabilis jalapa and the like are evergreen climbing shrubs with high ornamental value in the genus of Nyctaginaceae (Bougainavillea), and flowers are the main ornamental parts. Bougainvillea spectabilis originally found in brazil, has been introduced all over the world now, including southern provinces such as Fujian, Taiwan, Guangdong and the like in China, and is widely applied to urban greening. With the increasing importance of urban environmental beautification, bougainvillea spectabilis economic value and ornamental value are in urgent need of development.
When the bougainvillea spectabilis is applied to landscaping, the problems of vain growth of branches and leaves, disordered flowering phase, scattered plant types and poor ornamental value are frequently caused, the decoration and ornamental effect is seriously influenced, and the problem needs to be solved urgently. Therefore, foreign scholars have long studied the flowering mechanism, flower bud differentiation mechanism and flowering phase regulation technology of bougainvillea spectabilis. The university of California published an article in 1964 detailing its research efforts on the flowering mechanism of bougainvillea spectabilis. Hackett et al studied the morphological dissection of the inflorescence protomer of bougainvillea spectabilis and studied the systematic study of the flowering process and inflorescence structure of bougainvillea spectabilis by Lopez in order to investigate the morphological changes during the differentiation period of bougainvillea spectabilis. Early research results of foreign scholars preliminarily reveal the flowering process of bougainvillea spectabilis.
The bougainvillea spectabilis flowering phase regulating and controlling technology mainly belongs to two main categories, in the aspect of physical technology, Saifuddin and other researches show that the bougainvillea spectabilis is are increased in pruning times to promote more flower buds, Even-Chen and other researches show that the photosynthesis rate of mature leaves of bougainvillea spectabilis is improved when the bougainvillea spectabilis grows in a short-day environment, and Chenghuaqing researches show that the bract service lives of two varieties are obviously prolonged after shade domestication treatment is carried out on two varieties of 'purple flower' and 'Taibeihong'. In the aspect of chemical technology, Tse and the like simultaneously adopt two treatment methods of removing new leaves and cytokinin to research the influence of Tsingtazapine on the differentiation process of the trigonal plum blossom sequences. Selection of exogenous GA by Steffen et al3The effect on inflorescence differentiation was investigated. Saifuddin et al and Moneruzzaman et al used 100mg-1NAA, GA3The mixture is used for treating bougainvillea speetabilis, and meanwhile, the new leaves of the bougainvillea speetabilis are removed, so that the bract life can be remarkably prolonged, and the bract quality can be improved.
At present, researches on regulating and controlling the flowering phase of bougainvillea spectabilis mainly focused on the directions of variety breeding, flower bud differentiation, flower bud life, growth and development and the like. Researches are carried out on how to improve bougainvillea spectabilis flowering phase time, anthocyanidin content and the like, and a special nutrition regulator for regulating and controlling the bougainvillea spectabilis flowering phase is lacked, so that the technical problems of uneven flowering phase, uneven flower color and inconsistent quality standard in industrial production of bougainvillea spectabilis are urgently to be solved.
Disclosure of Invention
The invention aims to provide a composite nutrient regulator for regulating and controlling bougainvillea spectabilis florescence and a regulating and controlling method thereof.
The application provides a compound nutrition regulator for regulating and controlling bougainvillea spectabilis florescence, which consists of the following components: each 1L of purified water contains 100 μmol melatonin, 20-30mg ethephon, 1-2 g sucrose, and 2-4 g potassium dihydrogen phosphate.
Further, the compound nutrition regulator consists of the following components: every 1L of purified water contains 100 mu mol of melatonin, 20mg of ethephon, 2g of sucrose and 2g of monopotassium phosphate.
The application also provides a bougainvillea spectabilis florescence regulation and control method, which comprises the following steps:
(1) selecting annual potted bougainvillea spectabilis, and culturing at 24-26 deg.C and 70-80% humidity in room;
(2) the potting soil is a mixed matrix of nutrient soil, and the volume ratio is 1: 1;
(3) the concentration of melatonin is selected to be 100 mu mol/L, and distilled water is used for preparing a solution;
(4) carrying out 3-day seedling revival treatment on bougainvillea spectabilis, specifically watering in the morning and watering thoroughly, spraying 100 mu mol/L melatonin on the leaf surface of the bougainvillea spectabilis, and stopping watering after 3 days to enable the bougainvillea spectabilis to naturally dry for 14-21 days;
(5) after drought treatment, if wet watering is seen, spraying the compound nutrient regulator of claim 1 on the leaf surface every 2 days for 1 time at night until 1 month after flowering;
(6) fertilization is carried out during flowering, and topdressing is carried out for 1 time per week.
Further, stopping watering after 3 days in the step (4) to enable the plants to naturally dry for 14 days.
Further, bougainvillea spectabilis flowering period control treatment is performed around the first 50d of the expected flowering period of bougainvillea spectabilis.
Furthermore, the growth in the bougainvillea trigonata period needs to be under the conditions of full light and ventilation.
Further, the nutrient soil in the step (2) is prepared from coconut coir: peat: the volume ratio of the perlite is 1:4: 1.
Further, the base fertilizer of the pot soil in the step (2) is prepared by adding 15-9-11 g of Ordovician Green slow release fertilizer into each L of mixed matrix.
Further, in the flowering period in the step (6), nitrogen, phosphorus and potassium 20-20-20 water-soluble fertilizer is additionally applied for 1 time every 7 days, and the dilution concentration is 1000 times.
Has the advantages that:
1. the application provides a composite nutrient regulator for regulating and controlling bougainvillea spectabilis florescence and a regulating and controlling method thereof, which can promote bougainvillea spectabilis to bloom, shorten the time for blooming, prolong the whole florescence open time and greatly improve the ornamental value of the bougainvillea spectabilis.
2. Years of experiments show that the water control treatment is a key factor for prolonging the flowering phase of bougainvillea spectabilis, the bougainvillea spectabilis has moderate requirement on the water content of soil, and the flowering phase is difficult to control due to excessive water. The study finds that the melatonin can relieve the influence of plant drought stress, the mild drought stress and the melatonin treatment can obviously advance the flowering time of bougainvillea spectabilis through a large number of comparative tests, the gradient change presented by the soil water content of the bougainvillea spectabilis very obvious along with the extension of the drought time, the experiment finds that the ground diameter increment of the bougainvillea spectabilis treated by natural drought treatment (ZG) presents a descending trend along with the extension of the stress treatment time of the bougainvillea spectabilis, and the ground diameter increment of the bougainvillea spectabilis treated by the foliar melatonin spraying Treatment (TG) presents a descending trend after increasing. At the time of water cut 14d, the ZG-treated diameter begins to shrink, while the TG-treated diameter is still growing, at which point the increase in diameter reaches a maximum. Similarly, the seedling height also shows the same trend of change, and the melatonin treated bougainvillea glabra seedling height increment is the highest when the water supply is cut off for 14 d.
3. We find for the first time that melatonin and drought stress can shorten the differentiation time of bougainvillea spectabilis buds, obviously advance the flowering time of the bougainvillea spectabilis, cooperate with growth hormones such as ethephon, monopotassium phosphate and the like for promoting the differentiation of the bougainvillea spectabilis, further synergistically shorten the time required for flowering, simultaneously, sucrose can promote the synthesis of bougainvillea spectabilis pigments, the composite nutrition regulator is obtained after the components are compounded, and the composite nutrition regulator is used in the regulation and control of the flowering phase of bougainvillea spectabilis capable of obviously shortening the flowering time, prolonging the flowering phase time and brightening the color of the bougainvillea spectabilis.
4. The effect that the blooming is advanced by 37 days in the initial flowering period compared with the control group cultured by normal watering can be achieved through the joint regulation of drought stress and the compound nutrition regulator, and the blooming time from the full flowering period to the final flowering period is prolonged by 25 days compared with the control group. By different compound nutrient regulator compound formulas, a better test group is obtained, namely the drought stress of 14d is treated by adding melatonin than that of 21d, the flowering time can be obviously shortened, and the flowering period is prolonged.
5. In the control group which is only subjected to drought stress, or only subjected to drought stress plus melatonin treatment, or only sprayed with a plurality of compound nutritional regulators, the flowering time of the control group is delayed compared with that of the control group which uses the test example, and the flowering phase is shortened. For the control example using drought stress + melatonin treatment in the early stage, the control treatment using one component of the complex nutrient regulator alone all showed a delay in flowering time and a reduction in flowering time compared to the combination using the test examples of the present application. The effects of advancing flowering and delaying the flowering phase can be realized by jointly adjusting the drought stress and the composite nutrition regulator.
6. The composite nutrition regulator can regulate the flowering phase of bougainvillea spectabilis, can improve the content of anthocyanidin, is obtained by compounding different composite nutrition regulators according to different compounds, is singly compounded by one or more of melatonin, ethephon, cane sugar or potassium dihydrogen phosphate, and is sprayed on bougainvillea spectabilis leaves, the effect of the composite nutrition regulator prepared by singly using 100 mu mol/L MT +20mg/L E +2g/L S +2g/L P is good when the content of the anthocyanidin is not used, the flower color of the bougainvillea spectabilis enhanced by matching with drought stress treatment, and particularly when the full-bloom stage is open, the treatment is beneficial to the accumulation of the anthocyanidin in the bougainvillea spectaculata buds, the flower color is more bright, and the ornamental value is increased.
7. The matrix proportion and the fertilization measure can further promote flower bud differentiation in the bougainvillea spectabilis florescence, and the nutrient soil proportion is coconut coir: peat: the volume ratio of perlite is 1:4:1, 15-9-11 of Ordovician greens is used as a slow release fertilizer base fertilizer, 20-20-20 parts of nitrogen phosphorus and potassium are used for topdressing for 1 time per week, the differentiation of triangular plum blossom buds in a flowering period can be obviously promoted, the differentiation amount of the flower buds of new branches is promoted to be different by comparing the condition that topdressing is not carried out in a growing period and the condition that the topdressing frequency or the topdressing amount is different, and the number of flowers in the flowering period can be increased by matching with the flowering period regulation and control compound nutrition regulator for topdressing in the flowering period, so that the technical effect of improving the ornamental value is achieved. In different comparison examples of the Ordovician greens slow release fertilizer, the influence difference of the Ordovician greens slow release fertilizer on the flowering number is not obvious, and the influence difference of different treatments of substrate proportion and additional fertilizer on the flowering number of bougainvillea spectabilis is very obvious.
Drawings
FIG. 1 graph of changes in soil water content under natural drought stress
FIG. 2 is a graph showing the change of bougainvillea spectabilis ground diameter increment under drought stress and melatonin treatment
FIG. 3 change diagram of bougainvillea spectabilis seedling high increment under drought stress and melatonin treatment
Detailed Description
The processing method of the present invention will be described below with reference to specific examples, but the present invention is not limited thereto. The reagents and materials and equipment are commercially available, unless otherwise specified.
Example 1
A composite nutrition regulator for regulating bougainvillea spectabilis florescence comprises the following components: every 1L of purified water contains 100 mu mol of melatonin, 20mg of ethephon, 2g of sucrose and 2g of monopotassium phosphate.
Example 2
A composite nutrition regulator for regulating bougainvillea spectabilis florescence comprises the following components: every 1L of purified water contains 100 mu mol of melatonin, 30mg of ethephon, 2g of sucrose and 2g of monopotassium phosphate.
Example 3
A method for regulating bougainvillea spectabilis florescence, wherein the regulation treatment can be carried out at about the first 50d of the expected flowering period of bougainvillea spectabilis, and the method comprises the following steps:
(1) selecting annual potted bougainvillea spectabilis, and culturing at the indoor temperature of 25 ℃ and the indoor humidity of 70%;
(2) the potting soil is nutrient soil, coconut husk: peat: the volume ratio of the perlite is 1:4: 1; the base fertilizer of the pot soil is prepared by adding 15-9-11 g of ultragreen slow release fertilizer into each L of mixed matrix;
(3) the concentration of melatonin is selected to be 100 mu mol/L, and distilled water is used for preparing a solution;
(4) carrying out 3-day seedling reviving treatment on bougainvillea spectabilis, specifically watering and fully watering in the morning every day, spraying 100 mu mol/L melatonin on the leaf surface of the bougainvillea spectabilis in the evening, and stopping watering after 3 days to enable the bougainvillea spectabilis to be naturally dry for 14 days;
(5) after drought treatment, if the plants are watered wet, spraying the compound nutrition regulator of the embodiment 1 or 2 on the leaf surface for 1 time every 2 days at night until 1 month after flowering; the bougainvillea spectabilis is growing in a full-light and ventilated habitat state;
(6) topdressing nitrogen phosphorus potassium 20-20-20 water soluble fertilizer for 1 time every 7 days in the flowering period, diluting the fertilizer by 1000 times, and completely watering.
Test one: effect of drought stress and melatonin on morphological growth of bougainvillea spectabilis
The test method comprises the following steps:
annual potted bougainvillea spectabilis (hereinafter referred to as bougainvillea spectabilis) seedlings are used as test materials, all the seedlings grow strongly and are consistent in growth vigor, and no plant diseases or insect pests exist. The pot soil is a mixed matrix of nutrient soil, the bottom diameter of the flowerpot is 30cm, the height of the flowerpot is 40.5cm, and each seedling is potted. Before the experimental treatment, the bougainvillea spectabilis seedlings are transferred into a greenhouse, the indoor temperature is 24-26 ℃, and the humidity is 70-80%.
The test is divided into two treatments, namely natural drought treatment (ZG) and natural drought + foliage spray melatonin Treatment (TG), each treatment is repeated for 3 times, each treatment is repeated for 10 pots, and the prophase pretest shows that when 100 mu mol/L melatonin is sprayed on the leaves of the bougainvillea spectabilis seedlings, the growth state of the seedlings is the best, so that the concentration of the melatonin selected in the test is 100 mu mol/L, and distilled water is used for preparing a solution.
Before the test is started, 3 days of seedling revival are carried out on the bougainvillea spectabilis, two treated bougainvillea spectabilis are watered thoroughly at 9 points in the morning every day, and watering is stopped after 3 days to enable the bougainvillea spectabilis to be naturally dry; meanwhile, in the period of seedling recovering, 100 mu mol/L melatonin is sprayed on the surfaces of the TG-treated bougainvillea spectabilis leaves at 17:30 pm every day for 3 times, and the spraying amount is based on the standard that the leaves are fully distributed with water drops and do not flow in a strand manner. Judging the drought degree and the severe drought according to the relative water content of the soil: 15% -25%, moderate drought: 25% -35%, mild drought: 35 to 45 percent. Sampling is carried out once every 7d during drought, and sampling is carried out for measuring relevant indexes at 0, 7, 14, 21, 28 and 35 days respectively. Sampling on day 0 as a control group (CK), selecting bougainvillea spectabilis plants with excellent growth, observing the phenotypic change of the bougainvillea spectabilis plants, measuring the water content of soil, the plant height and the ground diameter of the bougainvillea spectabilis plants, and measuring again when sampling for the last time. The experiment was conducted in a modern intelligent greenhouse (120.63 ° E,28.00 ° N) in wenzhou city, zhejiang, 2021, month 10.
And (3) test results:
as shown in figure 1 (note: letters a, b, etc. indicate that the difference between the data is significant at the level of 0.05 according to the LSD test), after the bougainvillea spectabilis subjected to drought stress treatment, the soil water content of the bougainvillea spectabilis shows a continuous reduction trend, the water content in the CK period is 80.10%, the water content is reduced significantly in the 0d-14d after the drought treatment, and the reduction trend of the water content is gradually gentle in the later period of stress. In the test, when the water is cut off for 21 days, the mild drought stress level is reached, and the water content of the soil at the moment is 35.27%; when the water is stopped for 28 days, the moderate drought stress level is reached, and the water content is 27.65 percent; the severe drought stress level is reached when the water is cut off for 35 days, and the water content is 19.30 percent. Along with the prolonging of drought time, the gradient change presented by the water content of the bougainvillea spectabilis soil is very obvious, and the process of gradually losing the soil water in the natural environment is effectively reflected.
As shown in FIG. 2 (note: a, b, etc. letters indicate significance of difference at 0.05 level between data according to LSD test), the increment of bougainvillea speichi diameter by natural drought treatment (ZG) showed a decreasing trend with increasing time of stress treatment on bougainvillea speichi; at 28d (moderate) and 35d (severe) of water cut, the increase in the ground diameter showed a negative increase, indicating that drought stress inhibited the growth of bougainvillea glabra ground diameter and caused a certain amount of change in the external morphology of bougainvillea glabra.
As shown in fig. 2, the increase in the ground diameter of bougainvillea spectabilis treated by foliar melatonin Treatment (TG) showed a tendency of increasing first and then decreasing as the stress treatment time for bougainvillea spectabilis prolonged. At the time of water cut 14d, the ZG-treated diameter begins to shrink, while the TG-treated diameter is still growing, at which point the increase in diameter reaches a maximum.
As shown in FIG. 3 (note: letters a, b, etc. indicate significance of difference at the level of 0.05 between the data according to LSD test), the ZG-treated bougainvillea seedling height increment shows a trend of ascending first and then descending as the time for stress treatment on bougainvillea glabra is prolonged; at water cut 7d to 21d (mild), the seedling height increase showed a rising trend. The water is stopped for 21d (mild) to 35d (severe), and the seedling height increment shows a descending trend.
As shown in FIG. 3, the TG-treated bougainvillea spectabilis seedling high increment also shows a tendency of rising first and then falling as the stress treatment time for bougainvillea spectabilis lengthens. The increase in seedling height for TG treatment was higher than for ZG treatment at each stage in the experiment, by 3.9%, 9.2%, 7.1%, 5.1%, 0.5%, respectively. It shows that the melatonin treated bougainvillea was the highest in the increase of the height of bougainvillea spectabilis at 14d after water supply was stopped.
And (2) testing II: effect of drought stress and Complex Nutrition regulators on bouquet stage control
The test method comprises the following steps: the first test is continued, the test is carried out in 2021 year on day 1 and day 10, the drought stress + melatonin treatment is carried out, normal watering is used as a control (2 times of watering every week till the water is thoroughly watered), the treatment of the compound nutrition regulator is respectively carried out in 14 days and 21 days, specifically, the compound nutrition regulator as defined in claim 1 is sprayed on the leaf surface every noon and afternoon every 2 days until 1 month after the flowering, the treatment mode is shown in table 1, wherein each treatment is carried out for 3 times, each treatment is carried out for 10 pots, and the flowering time is observed. Recording the florescence: counting the date of initial flowering on the days that more than half of the plants are flowering; the full-bloom period counted by the day that more than 50% of the treated plants have flowers open; the final florescence date is counted by the day when more than 50% of flowers of each treated plant start wilting, and the flowering duration is counted by the number of days between the full florescence and the final florescence.
And (3) test results: the effect of flowering 37 days earlier than the initial flowering phase of the control group 1 can be achieved through the combined regulation of drought stress and the compound nutrition regulator, and the full-bloom period of the test example 1 is also 54 days longer than that of the control group 1, and the flowering time from the full-bloom period to the final flowering period is also 25 days longer than that of the control group 1(57 days). In experimental examples 2-4, by different compound nutrition regulator compound formulas, a better experimental group is obtained, namely the treatment of drought stress 14d plus melatonin can obviously shorten the time for flowering and prolong the flowering period. When the drought stress time is prolonged to 21d, the flowering time of bougainvillea spectabilis is also delayed because the growth and development of bougainvillea spectabilis are obviously influenced, and the flowering time is obviously shortened compared with the treatment of the drought stress 14 d.
In the control group subjected to only drought stress, or treated with only drought stress plus melatonin, or treated with only spraying of several kinds of composite nutrient regulators (control examples 2 to 4), it was found that the flowering time was delayed and the flowering period was shortened as compared with the flowering time of the combination using the test examples. For the control cases using drought stress + melatonin treatment in the early stage, the control treatments using one component of the complex nutritional regulator alone (see control cases 5 to 8) all showed a delay in flowering-time and a reduction in flowering-time compared to the combination using the test cases of the present application. The effects of advancing flowering and delaying the flowering phase can be realized by jointly adjusting the drought stress and the composite nutrition regulator.
TABLE 1 Effect of drought stress and Complex Nutrition regulators on bouquet control
Wherein: melatonin-MT, ethephon-E, sucrose-S, potassium dihydrogen phosphate-P
And (3) test III: influence of drought stress and composite nutrient regulator on bougainvillea spectabilis pigment content
The test method comprises the following steps:
the test was continued according to the method of test two, and the anthocyanidin was measured by taking the flower bud pieces at different periods, and the flower buds of each test group were randomly taken, and each treatment was repeated 3 times. The extraction and content determination method of the plant anthocyanidin is as follows:
the method comprises the following steps: weighing 1g of milled bougainvillea spectabilis bract, and soaking with 30ml of 5% ethanol at room temperature.
Step two: leaching with 5% ethanol 30ml at room temperature for three times, and filtering after 24 h.
Step three: concentrating the crude pigment extract, removing pectin with 75% ethanol, and vacuum evaporating to obtain concentrated anthocyanidin solution.
Step four: preparing a pigment solution to be detected from the pigment concentrated solution and 1.5mol/L HCl-95% ethanol according to a ratio of 15:85, measuring an absorbance value within a range of 550nm by using an ultraviolet-visible spectrophotometer, recording data, and analyzing to obtain an absorption spectrogram and a maximum absorption wavelength.
And (3) test results:
according to the comparison examples 5-10, the nutrition regulator obtained by compounding different composite nutrition regulators is obtained by singly using one or more of melatonin, ethephon, cane sugar or potassium dihydrogen phosphate, the effect of the formula of the application test example 1 is good when the nutrition regulator is sprayed on the leaves, the anthocyanidin content is not used, the effect is good, the nutrition regulator of 100 mu mol/L MT +20mg/L E +2g/L S +2g/L P is adopted to be matched with drought stress treatment, the flower color of bougainvillea is brightened, the anthocyanidin accumulation in the full-bloom stage is obviously promoted, and when the full-bloom stage is open, the treatment is beneficial to the anthocyanidin accumulation of the bougainvillea buds, the flower color is more bright, and the ornamental value is increased.
TABLE 2 Effect of Complex Nutrition Modulator on bougainvillea spectabilis pigment content
Wherein: melatonin-MT, ethephon-E, sucrose-S, potassium dihydrogen phosphate-P
And (4) testing: influence of matrix proportioning and fertilization measures on flower bud differentiation in bougainvillea spectabilis period
The test method comprises the following steps: setting different substrate proportions, fertilization modes and gradients, wherein the treatment modes are shown in a table 3, wherein each treatment mode is 3 times of repetition, each treatment mode is 10 times of repetition, and the flowering quantity is calculated on the 10 th day of entering the full-bloom period.
And (3) test results: the matrix formula and the fertilization measures of the test examples 1 and 2 are matched with the tests from the first to the third, and the flowering period regulation and control treatment of the composite nutrient additive can further promote the flower bud differentiation in the full-bloom period of the bougainvillea spectabilis, so that the flowering number is increased. The test of different substrate ratios shows that the coconut husk: peat: 1:4:1 of perlite, 15-9-11 of an ultramarine slow-release fertilizer and 20-20-20 of a nitrogen-phosphorus-potassium water-soluble fertilizer are added into a matrix, the blooming effect is optimal, bougainvillea spectabilis drought and waterlogging are afraid, years of researches show that coconut chaff and perlite are matched for use, the drainage of roots of the bougainvillea spectabilis can be promoted, the soil ponding is prevented, the coconut chaff can improve higher lignocellulose for the growth of the bougainvillea spectabilis, and the higher soil permeability and water retention capacity of the coconut chaff can promote the nutrition absorption of the roots of the bougainvillea spectabilis and prevent the waterlogging.
15-9-11 of Ordovician greens is used as a slow release fertilizer base fertilizer, and the nitrogen-phosphorus-potassium water soluble fertilizer is applied for 1 time per week in the flowering period, so that the differentiation of bouquets in the flowering period can be obviously promoted, the differentiation of the bouquets in the new branches is promoted differently by comparing the non-additional fertilizer in the growth period and the difference of the additional fertilizer frequency or the additional fertilizer amount, and the additional fertilizer is applied in the flowering period by matching with the flowering period regulation composite nutrition regulator disclosed by the application, so that the number of flowers in the flowering period can be increased, and the ornamental value can be improved.
In different comparison examples of the Ordovician greens slow release fertilizer, the influence difference of the Ordovician greens slow release fertilizer on the flowering number is not obvious, and the influence difference of different treatments of substrate proportion and additional fertilizer on the flowering number of bougainvillea spectabilis is very obvious.
TABLE 3 influence of substrate ratio and fertilization measures on flower bud differentiation in bougainvillea spectabilis
Although the present invention has been described with reference to a preferred embodiment, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (9)
1. The compound nutrition regulator for regulating and controlling bougainvillea spectabilis florescence is characterized by comprising the following components: each 1L of purified water contains 100 μmol melatonin, 20-30mg ethephon, 1-2 g sucrose, and 2-4 g potassium dihydrogen phosphate.
2. The compound nutritional regulator according to claim 1, wherein the compound nutritional regulator consists of: each 1L of purified water contains 100 mu mol of melatonin, 20mg of ethephon, 2g of sucrose and 2g of monopotassium phosphate.
3. A bougainvillea spectabilis florescence regulation and control method is characterized by comprising the following steps:
(1) selecting annual potted bougainvillea spectabilis, and culturing at 24-26 deg.C and 70-80% humidity in room;
(2) the potting soil is a mixed matrix of nutrient soil, and the volume ratio is 1: 1;
(3) the concentration of melatonin is selected to be 100 mu mol/L, and distilled water is used for preparing a solution;
(4) carrying out 3-day seedling revival treatment on bougainvillea spectabilis, specifically watering in the morning and watering thoroughly, spraying 100 mu mol/L melatonin on the leaf surface of the bougainvillea spectabilis, and stopping watering after 3 days to enable the bougainvillea spectabilis to naturally dry for 14-21 days;
(5) after drought treatment, if wet watering is seen, spraying the compound nutrient regulator of claim 1 on the leaf surface every 2 days for 1 time at night until 1 month after flowering;
(6) fertilization is carried out during flowering, and topdressing is carried out for 1 time per week.
4. The method for regulating bougainvillea spectabilis flowering phase according to claim 3, wherein watering is stopped after 3 days in step (4) to allow the bougainvillea spectabilis to naturally dry for 14 days.
5. The method of controlling bougainvillea spectabilis flowering phase according to claim 3, wherein bougainvillea spectabilis flowering phase control treatment is performed in about the first 50d of the expected flowering phase of bougainvillea spectabilis.
6. The method of bougainvillea spectabilis flowering stage control as claimed in claim 3 wherein bougainvillea spectabilis is grown in a full-light and ventilated habitat.
7. The method for regulating bougainvillea spectabilis florescence according to claim 3, wherein the nutrient soil in the step (2) is coconut coir: peat: the volume ratio of the perlite is 1:4: 1.
8. The method for regulating and controlling the flowering phase of bougainvillea spectabilis according to claim 3, wherein the base fertilizer of the pot soil in the step (2) is 15-9-11 g of ultragreen slow release fertilizer added to each L of mixed matrix.
9. The method for regulating bougainvillea spectabilis flowering phase according to claim 3, wherein the water-soluble fertilizer containing NPK 20-20-20 is additionally applied every 7 days in the flowering phase in step (6) at a dilution concentration 1000 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210182505.9A CN114560736B (en) | 2022-02-25 | 2022-02-25 | Composite nutrition regulator for regulating bougainvillea spectabilis florescence and regulating method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210182505.9A CN114560736B (en) | 2022-02-25 | 2022-02-25 | Composite nutrition regulator for regulating bougainvillea spectabilis florescence and regulating method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114560736A true CN114560736A (en) | 2022-05-31 |
| CN114560736B CN114560736B (en) | 2022-11-22 |
Family
ID=81715232
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210182505.9A Active CN114560736B (en) | 2022-02-25 | 2022-02-25 | Composite nutrition regulator for regulating bougainvillea spectabilis florescence and regulating method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114560736B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115490554A (en) * | 2022-10-20 | 2022-12-20 | 浙江省亚热带作物研究所(浙南林业科学研究院) | Lagerstroemia indica flowering phase regulating and controlling composite plant regulator and flowering phase regulating and controlling method |
| CN116686664A (en) * | 2023-08-04 | 2023-09-05 | 四川嘉智生态科技有限公司 | Method for regulating and controlling field planting triangle plum blossom period |
| CN116711731A (en) * | 2023-08-07 | 2023-09-08 | 四川嘉智生态科技有限公司 | Regulator for prolonging flowering phase of bougainvillea spectabilis and application method thereof |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004083459A (en) * | 2002-08-26 | 2004-03-18 | Shintou Something:Kk | Plant activator comprising melatonin as active ingredient |
| CN105638252A (en) * | 2015-12-31 | 2016-06-08 | 佛山铭乾科技有限公司 | Water controlling and flower promoting method for bougainvillea spectabilis willd |
| CN106035336A (en) * | 2016-07-05 | 2016-10-26 | 中国农业科学院油料作物研究所 | Complex hormone and preparation method and application thereof |
| CN107580940A (en) * | 2017-09-07 | 2018-01-16 | 南宁市园林科研所 | A kind of method of Triangular Flos Mume period regulation |
| CN108129214A (en) * | 2018-02-26 | 2018-06-08 | 江苏省农业科学院 | It is a kind of that day lily is promoted to do sth. in advance the growth regulator buddingged |
| CN109566621A (en) * | 2018-11-27 | 2019-04-05 | 中国农业大学 | A kind of concentration complexing agent and the preparation method and application thereof at regulation plant florescence |
| CN112166923A (en) * | 2020-09-18 | 2021-01-05 | 广州普邦园林股份有限公司 | Method for regulating flowering of bougainvillea spectabilis |
| CN114208576A (en) * | 2021-12-24 | 2022-03-22 | 漳州环境集团有限公司 | Bougainvillea spectabilis flower control method |
-
2022
- 2022-02-25 CN CN202210182505.9A patent/CN114560736B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004083459A (en) * | 2002-08-26 | 2004-03-18 | Shintou Something:Kk | Plant activator comprising melatonin as active ingredient |
| CN105638252A (en) * | 2015-12-31 | 2016-06-08 | 佛山铭乾科技有限公司 | Water controlling and flower promoting method for bougainvillea spectabilis willd |
| CN106035336A (en) * | 2016-07-05 | 2016-10-26 | 中国农业科学院油料作物研究所 | Complex hormone and preparation method and application thereof |
| CN107580940A (en) * | 2017-09-07 | 2018-01-16 | 南宁市园林科研所 | A kind of method of Triangular Flos Mume period regulation |
| CN108129214A (en) * | 2018-02-26 | 2018-06-08 | 江苏省农业科学院 | It is a kind of that day lily is promoted to do sth. in advance the growth regulator buddingged |
| CN109566621A (en) * | 2018-11-27 | 2019-04-05 | 中国农业大学 | A kind of concentration complexing agent and the preparation method and application thereof at regulation plant florescence |
| CN112166923A (en) * | 2020-09-18 | 2021-01-05 | 广州普邦园林股份有限公司 | Method for regulating flowering of bougainvillea spectabilis |
| CN114208576A (en) * | 2021-12-24 | 2022-03-22 | 漳州环境集团有限公司 | Bougainvillea spectabilis flower control method |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115490554A (en) * | 2022-10-20 | 2022-12-20 | 浙江省亚热带作物研究所(浙南林业科学研究院) | Lagerstroemia indica flowering phase regulating and controlling composite plant regulator and flowering phase regulating and controlling method |
| CN115490554B (en) * | 2022-10-20 | 2023-11-24 | 浙江省亚热带作物研究所(浙南林业科学研究院) | Crape myrtle flowering phase regulation composite plant regulator and flowering phase regulation method |
| CN116686664A (en) * | 2023-08-04 | 2023-09-05 | 四川嘉智生态科技有限公司 | Method for regulating and controlling field planting triangle plum blossom period |
| CN116686664B (en) * | 2023-08-04 | 2023-10-24 | 四川嘉智生态科技有限公司 | Method for regulating and controlling field planting triangle plum blossom period |
| CN116711731A (en) * | 2023-08-07 | 2023-09-08 | 四川嘉智生态科技有限公司 | Regulator for prolonging flowering phase of bougainvillea spectabilis and application method thereof |
| CN116711731B (en) * | 2023-08-07 | 2023-10-20 | 四川嘉智生态科技有限公司 | Regulator for prolonging flowering phase of bougainvillea spectabilis and application method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114560736B (en) | 2022-11-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN114560736B (en) | Composite nutrition regulator for regulating bougainvillea spectabilis florescence and regulating method thereof | |
| CN113906944B (en) | Bougainvillea spectabilis florescence regulation and control method | |
| CN105594573B (en) | A kind of method for shortening camellia commodity potted flower juvenile phase | |
| CN104686183A (en) | Efficient elderberry cultivation method | |
| CN112006023A (en) | Medicament for regulating flowering phase of garlic and rattan and regulating method thereof | |
| CN114208618B (en) | Method for improving peony propagation efficiency by promoting cultivation in two seasons in one year | |
| CN104982177B (en) | A kind of implantation methods of violet | |
| CN115490554B (en) | Crape myrtle flowering phase regulation composite plant regulator and flowering phase regulation method | |
| KR20010097002A (en) | A production methode of small division flower use flowering shrub | |
| DK181430B1 (en) | Method for high-efficiently inducing double stems of phalaenopsis | |
| CN111642351A (en) | Method for promoting early flowering of German iris and application | |
| CN112616542B (en) | Flower control cultivation method for sophora japonica | |
| Oh et al. | Growth characteristics by plug tray cell size, soil type, and fertilizer concentration for plug seedling production of Veronica pusanensis YN Lee | |
| CN110178681B (en) | Planting method beneficial to growth and flowering of corydalis angulata | |
| CN113678830A (en) | Composition for improving watermelon fruit quality and application thereof | |
| CN1234264C (en) | Technology for culturing and collecting seeding of Camptotheca acuminata Decne | |
| NL2028355B1 (en) | Container seedling substrate for lithocarpus polystachyus and seedling raising method | |
| CN116034700B (en) | Method for precisely applying nitrogen fertilizer in whole growth period of cut chrysanthemum | |
| NL2028853B1 (en) | Method for rapidly cultivating miniature potted camellia l. | |
| CN113854058B (en) | Method for improving drought resistance and salt resistance of fringed pink | |
| CN112703947B (en) | Elaeagnus angustifolia arborescent method and application | |
| CN110818515B (en) | Blueberry nutrient solution for indoor cultivation and preparation method and application thereof | |
| CN110249889B (en) | Manual planting method of taxus chinensis | |
| CN115362902A (en) | Container cultivation method for 'Luoyang red' peony | |
| CN106358916A (en) | Method for potting nandina |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |