CN1234264C - Technology for culturing and collecting seeding of Camptotheca acuminata Decne - Google Patents
Technology for culturing and collecting seeding of Camptotheca acuminata Decne Download PDFInfo
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- CN1234264C CN1234264C CN 200310107711 CN200310107711A CN1234264C CN 1234264 C CN1234264 C CN 1234264C CN 200310107711 CN200310107711 CN 200310107711 CN 200310107711 A CN200310107711 A CN 200310107711A CN 1234264 C CN1234264 C CN 1234264C
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Abstract
The present invention relates to a camptotheca acuminata seedling cultivation collection technology. Seedlings taken from camptotheca acuminata seeds of the current year are used as the seedling source, and the camptotheca acuminata seedlings which grow out euphylla in early spring are transplanted in a field with the plant spacing and the row spacing of 5 to 50 centimeters; after the camptotheca acuminata seedlings are transplanted in the field for 5 to 25 days, the Nitrogen fertilizer is added to the field; in the process of camptotheca acuminata seedling growth, irrigation is carried out everyday to every three days, and the soil water content is kept at 50 to 80%; in the process of the field cultivation, branches and leaves on the ground are collected every 1 to 4 months; the complete plants are collected after the growing season. The present invention provides a cultivation collection technique for camptotheca acuminata seedling cultivation in non-camptotheca acuminata natural distribution areas, the acquisition amount of the plant material is high, and the present invention enhances the camptothecin content.
Description
Technical field
The present invention relates to the cultivation method of woody plant, relating in particular to a kind of is the cultivation and collection technology of the Camptotheca acuminata seedlings in seedling source to give birth to camptotheca seed gained seedling then.
Background technology
Camplotheca acuminata (Camptotheca acuminata Decaisne) is that Nyssaceae (also claims Nyssaceae, Nyssaceae) camplotheca acuminata belongs to (Camptotheca) plant, be the peculiar tropical trees of China, happiness warm and moist weather, be distributed widely in China Yangtze river basin and on the south the each province.
Camptothecine (Camptothecin, CPT) be from camplotheca acuminata, to separate obtain a kind of kinds of tumor cells is all had obvious inhibiting alkaloid, and anticancer mechanism uniqueness (camptothecine is the topmost topoisomerase I inhibitor of finding up to now) is present, and the camptothecine of existing 2 kinds of high-efficiency low-toxicities obtains drugs approved by FDA and is applied to the various cancers of clinical treatment.The camplotheca acuminata complete stool all contains camptothecine.At present, camplotheca acuminata is a primary raw material of producing camptothecine.
The cultivation method that Patent Office of State Intellectual Property Office discloses a kind of complotheca acuminata ultra-short period ecological economic coppice January 31 calendar year 2001, this patent of invention is according to the terrain condition, different gradient is adjusted land preparation mode, after the afforestation first, second and third stump in year autumn, plucked the top on the 4th year, restriction camplotheca acuminata height is below 180 centimetres, and the initial stage dense planting played interlacing on the 3rd year or every the strain intermediate cutting.Above-mentioned camptotheca acuminata decne cultivation method is only applicable to the natural distribution district of camplotheca acuminata, can't promote at northern wide geographic area, and this cultivation method does not relate to the cultivation step that improves camptothecine content in the camplotheca acuminata only at the foundation of its stunted growth pattern.
Summary of the invention
The object of the present invention is to provide and a kind ofly can cultivate Camptotheca acuminata seedlings in non-camplotheca acuminata natural distribution district, improve the cultivation and collection technology of the high Camptotheca acuminata seedlings of the acquisition amount of camptothecine content in the Camptotheca acuminata seedlings and vegetable material.
In order to achieve the above object, be the seedling source to give birth to camptotheca seed gained seedling then, in the early spring, the camplotheca acuminata seedling that grows true leaf is transplanted into the land for growing field crops with 5～50 centimetres spacing in the rows and line-spacing; Be transplanted into the land for growing field crops after 5～25 days, append nitrogenous fertilizer 1 time; In the Camptotheca acuminata seedlings process of growth, irrigated in per 1～3 day, keeping soil moisture content is 50～80%; In the field production process per 1～4 month, the branch and the blade of the acrial part of gathering; Complete stool is gathered after growth season finishes.
Advantage of the present invention is:
1. the present invention is the seedling source to give birth to camptotheca seed gained seedling then, with a Ji Weiyi cultivation cycle of growth, remove the Camptotheca acuminata seedlings cultivation that is applicable to camplotheca acuminata natural distribution district, be particularly useful for cultivating Camptotheca acuminata seedlings, for camptothecine production is supplied raw materials in non-camplotheca acuminata natural distribution district.
2. the present invention can improve the acquisition amount of vegetable material, and gained vegetable material total amount manys 30% than what repeatedly gather under the same case.Amount than do not carry out arid under the same terms and the raw material handled of sheltering from heat or light high by 50%.
3. the present invention carries out before gathering that short-term is sheltered from heat or light and arid is handled, and neither can suppress the Camptotheca acuminata seedlings growth, can improve the camptothecine content in the Camptotheca acuminata seedlings again.In the gained raw material camptothecine content than do not carry out arid under the same terms and the raw material handled of sheltering from heat or light high by 50%.
Embodiment
Describe in further detail below in conjunction with embodiments of the invention:
The cultivation and collection technology of Camptotheca acuminata seedlings is the seedling source to give birth to camptotheca seed gained seedling then, and the camplotheca acuminata seedling that grows true leaf the early spring is transplanted into the land for growing field crops with 5～50 centimetres spacing in the rows and line-spacing; Be transplanted into the land for growing field crops after 5～25 days, append nitrogenous fertilizer 1 time; In the Camptotheca acuminata seedlings process of growth, irrigated in per 1～3 day, keeping soil moisture content is 50～80%; In the field production process per 1～4 month, the branch and the blade of the acrial part of gathering; Complete stool is gathered after growth season finishes.
The cultivation and collection technology of described Camptotheca acuminata seedlings, the nitrogenous fertilizer weight of appending are 25～60 kilograms on every mu of ground.
The cultivation and collection technology of described Camptotheca acuminata seedlings, during the Camptotheca acuminata seedlings of gathering in the cultivation process, 10～20 centimetres of reservation top plant heights.
The cultivation and collection technology of described Camptotheca acuminata seedlings, Camptotheca acuminata seedlings acrial part 3～10 days before gathering stop to irrigate in the cultivation process, processings of sheltering from heat or light simultaneously, the back of gathering is removed and is sheltered from heat or light, the recovery normal irrigation.
The cultivation and collection technology of described Camptotheca acuminata seedlings, the intensity of illumination of sheltering from heat or light after handling is 50～80% of full exposure.
The cultivation and collection technology of described Camptotheca acuminata seedlings was gathered before the Camptotheca acuminata seedlings 5～15 days finish preparing complete stool growth season, stops to irrigate, and the processing of sheltering from heat or light.
The cultivation and collection technology of described Camptotheca acuminata seedlings, the intensity of illumination of sheltering from heat or light after handling is 30～60% of full exposure.
The cultivation and collection technology of described Camptotheca acuminata seedlings is sheltered from heat or light to handle and is used plastics shade net, colourless filter coating, colour light filter film.
Embodiment 1
During the early spring, the camplotheca acuminata seedling that grows true leaf is transplanted into the land for growing field crops, spacing in the rows and line-spacing are 15 centimetres.After 15 days, every mu of ground applies 35 kilograms of nitrogenous fertilizer.Irrigated once in per 1 day, and kept soil moisture content more than 70%.In the Camptotheca acuminata seedlings process of growth, irrigate and shelter from heat or light every stopping in 4 weeks with shade net, make intensity of illumination remain on full exposure about 40% once, handle after 3 days gather overground part branch and blade, and keep overground part plant about 15 centimetres.Finish to prepare all to gather preceding 10 days in growth season, stop to irrigate and, make intensity of illumination remain on about 50% of full exposure, handle and carry out the Camptotheca acuminata seedlings complete stool after 10 days and gather with the processing of sheltering from heat or light of plastics shade net.
Embodiment 2
During the early spring, the camplotheca acuminata seedling that grows true leaf is transplanted into the land for growing field crops, spacing in the rows and line-spacing are 8 centimetres.After 5 days, every mu of ground applies 50 kilograms of nitrogenous fertilizer.Irrigated once in per 2 days, and kept soil moisture content more than 60%.In the Camptotheca acuminata seedlings process of growth, irrigate and shelter from heat or light every stopping in 6 weeks with colourless filter coating, make intensity of illumination remain on full exposure about 60% once, handle after 4 days gather overground part branch and blade, and keep overground part plant about 20 centimetres.Finish to prepare all to gather preceding 10 days in growth season, stop to irrigate and, make intensity of illumination remain on about 55% of full exposure, handle and carry out the Camptotheca acuminata seedlings complete stool after 15 days and gather with the shade net processing of sheltering from heat or light.
Embodiment 3
During the early spring, the camplotheca acuminata seedling that grows true leaf is transplanted into the land for growing field crops, spacing in the rows and line-spacing are 20 centimetres.After 25 days, every mu of ground applies 30 kilograms of nitrogenous fertilizer.Irrigated once in per 3 days, and kept soil moisture content more than 50%.In the Camptotheca acuminata seedlings process of growth, irrigate and shelter from heat or light every stopping in 8 weeks with red filter coating, make intensity of illumination remain on full exposure about 80% once, handle after 6 days gather overground part branch and blade, and keep overground part plant about 20 centimetres.Finish to prepare all to gather preceding 8 days in growth season, stop to irrigate and, make intensity of illumination remain on about 40% of full exposure, handle and carry out the Camptotheca acuminata seedlings complete stool after 8 days and gather with the processing of sheltering from heat or light of red filter coating.
Embodiment 4
During the early spring, the camplotheca acuminata seedling that grows true leaf is transplanted into the land for growing field crops, spacing in the rows and line-spacing are 25 centimetres.After 10 days, every mu of ground applies 25 kilograms of nitrogenous fertilizer.Irrigated once in per 3 days, and kept soil moisture content more than 50%.In the Camptotheca acuminata seedlings process of growth, irrigate and shelter from heat or light every stopping in 10 weeks with green filter film, make intensity of illumination remain on full exposure about 50% once, handle after 3 days, gather overground part branch and blade keep about 10 centimetres overground part plant.Finish to prepare all to gather preceding 5 days in growth season, stop to irrigate and, make intensity of illumination remain on about 35% of full exposure, handle and carry out the Camptotheca acuminata seedlings complete stool after 5 days and gather with the green filter film processing of sheltering from heat or light.
Claims (7)
1. the cultivation and collection method of a Camptotheca acuminata seedlings is characterized in that: to give birth to camptotheca seed gained seedling then is the seedling source, and the camplotheca acuminata seedling that grows true leaf the early spring is transplanted into the land for growing field crops with 5～50 centimetres spacing in the rows and line-spacing; Be transplanted into the land for growing field crops after 5～25 days, append nitrogenous fertilizer 1 time; In the Camptotheca acuminata seedlings process of growth, irrigated in per 1～3 day, keeping soil moisture content is 50～80%; In the field production process per 1～4 month, the branch and the blade of the acrial part of gathering; Complete stool is gathered after growth season finishes.
2. according to the cultivation and collection method of the described Camptotheca acuminata seedlings of claim 1, it is characterized in that: described nitrogenous fertilizer weight of appending is 25～60 kilograms on every mu of ground.
3. according to the cultivation and collection method of the described Camptotheca acuminata seedlings of claim 1, it is characterized in that: during the Camptotheca acuminata seedlings of gathering in the cultivation process, 10～20 centimetres of reservation top plant heights.
4. according to the cultivation and collection technology of claim 1 or 3 described Camptotheca acuminata seedlings, it is characterized in that: Camptotheca acuminata seedlings acrial part 3～10 days before gathering in the cultivation process, stop to irrigate, processings of sheltering from heat or light simultaneously, the back of gathering is removed and is sheltered from heat or light, the recovery normal irrigation.
5. according to the cultivation and collection method of the described Camptotheca acuminata seedlings of claim 4, it is characterized in that: the intensity of illumination of sheltering from heat or light after handling is 50～80% of full exposure.
6. according to the cultivation and collection method of the described Camptotheca acuminata seedlings of claim 1, it is characterized in that: gathered before the Camptotheca acuminata seedlings 5～15 days finish preparing complete stool growth season, stop to irrigate, and the processing of sheltering from heat or light.
7. according to the cultivation and collection method of the described Camptotheca acuminata seedlings of claim 6, it is characterized in that: the intensity of illumination of sheltering from heat or light after handling is 30～60% of full exposure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310107711 CN1234264C (en) | 2003-11-20 | 2003-11-20 | Technology for culturing and collecting seeding of Camptotheca acuminata Decne |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310107711 CN1234264C (en) | 2003-11-20 | 2003-11-20 | Technology for culturing and collecting seeding of Camptotheca acuminata Decne |
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| Publication Number | Publication Date |
|---|---|
| CN1543770A CN1543770A (en) | 2004-11-10 |
| CN1234264C true CN1234264C (en) | 2006-01-04 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 200310107711 Expired - Fee Related CN1234264C (en) | 2003-11-20 | 2003-11-20 | Technology for culturing and collecting seeding of Camptotheca acuminata Decne |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100913776B1 (en) * | 2007-11-21 | 2009-08-26 | 전라남도 | Method of Producing Camtothecin |
| CN101519325B (en) * | 2009-04-10 | 2011-12-21 | 广东省中药研究所 | Foliage promoter for improving camptothecine content of camptotheca acuminata |
| CN101548618B (en) * | 2009-05-11 | 2010-12-29 | 广东省中药研究所 | Camptotheca acuminata decne cultivation method for improving camptothecine content |
| CN105009923B (en) * | 2015-08-12 | 2017-08-01 | 南京林业大学 | A kind of camplotheca acuminata cuttage propagation method |
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- 2003-11-20 CN CN 200310107711 patent/CN1234264C/en not_active Expired - Fee Related
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| Publication number | Publication date |
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