DK181430B1 - Method for high-efficiently inducing double stems of phalaenopsis - Google Patents

Method for high-efficiently inducing double stems of phalaenopsis Download PDF

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DK181430B1
DK181430B1 DKPA202270523A DKPA202270523A DK181430B1 DK 181430 B1 DK181430 B1 DK 181430B1 DK PA202270523 A DKPA202270523 A DK PA202270523A DK PA202270523 A DKPA202270523 A DK PA202270523A DK 181430 B1 DK181430 B1 DK 181430B1
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phalaenopsis
plants
stem
pedicel
stems
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DKPA202270523A
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Huang Guiyun
Zhang Haibo
Wu Di
Wu Jinhua
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China Three Gorges Corp
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
    • A01H3/04—Processes for modifying phenotypes, e.g. symbiosis with bacteria by treatment with chemicals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/02—Flowers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/04—Stems
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/62—Orchidaceae [Orchid family]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
    • A01N43/647—Triazoles; Hydrogenated triazoles
    • A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/26—Phosphorus; Compounds thereof

Abstract

The present invention relates to the technical field of plant handling, in particular to a method for high-efficiently inducing double stems of Phalaenopsis. Phalaenopsis can be induced with double stems quickly and efficiently after combined treatment with chemical agents (TDZ(0.01~0.05g/L)+GA3(0.3~1.0g/L)+IAA(0.1~0.6g/L)+PP333(0.2~0.8g/L)+P2O5(0.5~1.2g/L)+ glutamic acid(0.8~1.5g/L)). This method is simple to implement and saves time and effort, providing a fast and effective way for Phalaenopsis to improve product quality and enhance the ornamental value of Phalaenopsis. This method is suitable for flower forcing of phalaenopsis in a large scale with low production costs, the resultant phalaenopsis has characteristics of high quality and good consistency, and advantages of low input and high income, and shows good popularization and application value.

Description

DK 181430 B1 1
METHOD FOR HIGH-EFFICIENTLY INDUCING DOUBLE STEMS OF
PHALAENOPSIS
FIELD OF THE INVENTION
The present invention relates to the technical field of plant handling, in particular to a method for high-efficiently inducing double stems of Phalaenopsis.
BACKGROUND OF THE INVENTION
Phalaenopsis is a Phalaenopsis genus plant in the Orchid family, known as the "Queen of
Foreign Orchid" with peculiar flower shape, gorgeous color and long-lasting florescence.
The presentation "Investigating the spiking and double spiking ratio of Phalaenopsis within the air-inflated double-poly envelope inside a plastic greenhouse", hips: //wenku baidu. com/view/eSeRe35303020740belen50e32eaS 518 11200960¢acgdd=c5¢8¢ 3830302074Dbele6S0eS2ea5 5181 a0cR0c& fr=catalog Main & wkis =1688543054863, dated October 19, 2006 and prepared by Yaoren, Liu, said that after more than 30 days of flower forcing the stem-doubling induction rates of different Phalaenopsis varieties were 50% of Sunset Red, 27% of Lawrence, 17% of V3, and only 3% of Jubao Red Rose. The article "Why Phalaenopsis is the king of New Year's eve flowers" carried by "China Flower &
Gardening News", March 28, 2020, said that the amount of the Phalaenopsis put on the — market during the New Year's eve in 2020 in China is estimated to exceed 55 million, and the total sales amount is expected to be more than 1 billion yuan, which is the sum of the sales amounts of several other major varieties ranked behind. In the aspect of double-stem phalaenopsis varieties, Europe has a great advantage over China, as European breeders have cultivated double-stem varieties for more than 20 years. For middle and small flower shapes, — double-stem varieties are more economical for growers than single-stem varieties. For distribution customers in China, while the buying price of single-stem Phalaenopsis products is 20 yuan, the buying price of high-quality double-stem products is generally 25 yuan at a maximum premium of 25%. Thus, it can be seen that it is of important practical significance to perfect the research of double-stem Phalaenopsis for improving people's life quality and developing China into a beautiful country.
DK 181430 B1 2
SUMMARY OF THE INVENTION
In order to solve the above-mentioned technical problems, the present invention provides a method for high-efficiently inducing double stems of Phalaenopsis, which enables people to enjoy the sight of cultivating double-stem varieties, but greatly saves the time and — costs required for breeding.
The technical solution of the present invention is as follows.
A method for high-efficiently inducing double stems of Phalaenopsis includes the following steps:
S1. physical processing of plants: uniformly adjusting the physical plane and spatial angle of Phalaenopsis plants to be processed for flower forcing; and
S2. processing with a chemical reagent: after completing S1, spraying a pedicel-inducing mixed solution on leaves and stem segments of the Phalaenopsis plants; ending with enabling double stems of Phalaenopsis to be high-efficiently induced.
In S1, the step of adjusting the physical plane includes arranging all Phalaenopsis plants in an identical orientation with the growth direction of the stems and leaves of plants toward north and their roots toward south.
In S1, the step of adjusting the spatial angle includes raising the inclination of all the
Phalaenopsis plants to be processed for flower forcing to an identical angle, so as enable light to strike the leaf axils of the stem segments of 3-5 leaves of the Phalaenopsis plants from top — to bottom, and after growing the plants 10-20 cm for pedicels under flower forcing for 30 to 40 days, removing the inclination and returning to arrangements at 0 or 180 degrees.
In S2, the pedicel-inducing mixed solution includes TDZ (0.01~0.05g/L)+
GA3(0.3~1.0g/L)+IAA(0.1~0.6g/L)+PP333(0.2~0.8g/L)+P205(0.5~1.2g/L)+ glutamic acid (0.8~1.5g/L).
Preferably, in S2, the spraying method includes using a 150 ml small sprinkler each time, and adjusting its mouth to the best state of forming mists applied in one cycle around the stem segments of 2-5 leaves of the Phalaenopsis plants, ensuring that the leaf axils of the plants are sprayed, such that after spraying, the water mist adheres to the surface of the stem segments and does not flow.
Preferably, in S2, the pedicel-inducing mixed solution is applied once every 9-12 days 2-4 times in total.
DK 181430 B1 3
The present invention has beneficial effects as follows. 1 Plants can be processed simply and effectively outdoor, and the double stems of
Phalaenopsis can be induced periodically. 2.The adjustment of the physical plane of the Phalaenopsis plants can not only make earth's magnetic field pass through the plant bodies smoothly, but also minimize the impact of the magnetic field on the plant, facilitating the operations such as fertilizing, watering and changing pots. 3.The adjustment of the physical spatial angle of the Phalaenopsis plants helps light to strike on the leaf axils of the stem segments of 3-5 leaves of the Phalaenopsis plants from top to bottom, promoting the germination of double stems. 4 The chemical processing can effectively boost the plants to change form vegetative growth to reproductive growth, and promote flower-bud differentiation and double stem rate, so it is of great significance in large-scale production.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG.1 shows the consistent orientation of the planes of the Phalaenopsis leaves.
FIG. 2 shows the processing of the spatial inclination to the Phalaenopsis plants.
FIG.3 shows the induction of the double stems of the Phalaenopsis plant group.
FIG 4 shows the induction of the double stems of the single Phalaenopsis plant.
FIG.5 shows the flowering plant of the double-stem Phalaenopsis in the color system of
Red (Red Sun).
FIG.6 shows the flowering plant of the double-stem Phalaenopsis in the color system of
Yellow (Richness-Happiness Sunset).
FIG.7 shows the flowering plant of the double-stem Phalaenopsis in the color system of
Orange (Golden Butterfly).
FIG.8 shows the flowering plant of the double-stem Phalaenopsis in the color system of
Red (Century Red).
DETAILED DESCRIPTION OF SOME EMBODIMENTS
In order to make the objective, technical solution and advantages of the present invention clearer, we will further detail the present invention in combination with the — examples as follows, however, it should be understood that the specific examples described herein are only used to explain the present invention, but not to pose any limitation on the
DK 181430 B1 4 present invention.
Example I 1. Physical Processing of Plants:
In order to promote the germination of double stems smoothly and effectively, we physically process the Phalaenopsis plants in advance, that is, uniformly adjust the physical plane and spatial angle of the Phalaenopsis plants to be processed for flower forcing. Firstly, the step of adjusting the physical plane includes arranging all Phalaenopsis plants toward an identical orientation, this trial greenhouse is in the north-south orientation(other similar greenhouses can be adjusted according to the actual situation), which orientates the growth direction of the stems and leaves of the plants toward north and its roots toward south, not only guaranteeing the growth of the plants along the earth's magnetic field and the minimization of the impact of the magnetic field on the plant, but also facilitating the operations such as fertilizing, watering and changing pots (see FIG.1). Secondly, the step of arranging all Phalaenopsis plants toward an identical orientation is followed by the step of adjusting the spatial angle, which includes raising the inclination of all the Phalaenopsis plants to be processed for flower forcing to an identical angle, so as to incline the plants toward the bottom surface of a swayed bed at an angle of 70 to 90 degrees and then enable light to strike the leaf axils of the stem segments of 3~5 leaves of the Phalaenopsis plants from top to bottom, after growing to 10~20cm for pedicels under flower forcing for 30 to 40 — days, removing the inclination and returning to the arrangement at 0 or 180 degrees (see
FIG.2). The detailed experimental results are shown in Tables 1-3.
According to the statistical analysis after 30 days, the stem-doubling induction results of the Phalaenopsis plants processed toward an identical orientation show that: no inducted double stem occurs in the Phalaenopsis grandiflora in the group Pi only processed toward an identical orientation, as 0% as a stem-doubling induction rate; 5 inducted double stems occur in the Phalaenopsis floweret in the group P» processed toward an identical orientation, as 5.6% as a stem-doubling induction rate; no inducted double stem still occurs in the
Phalaenopsis grandiflora in the control group Ps, as 0% as a stem-doubling induction rate; and 1 inducted double stem occurs in the Phalaenopsis floweret in the control group Ps, as 1.1% as a stem-doubling induction rate (see Table 1).
According to the statistical analysis after 30 days, the stem-doubling induction results of
DK 181430 B1 the Phalaenopsis plants spatially inclined at an angle show that: 4 inducted double stems occur in the Phalaenopsis grandiflora in the group Ps only spatially inclined at an angle of 70 to 90 degrees, as 4.4% as a stem-doubling induction rate; 9 inducted double stems occur in the Phalaenopsis floweret in the group Ps spatially inclined at an angle of 70 to 90 degrees, as 5 10.0% as a stem-doubling induction rate; the Phalaenopsis grandiflora in the group Ps has 4 double stems more than that in the control group Ps, with the stem-doubling induction rate increased by 4.4%, and the Phalaenopsis floweret in the group Ps has 8 double stems more than that in the control group Ps, with the stem-doubling induction rate increased by 8.9% (see Table 2).
According to the statistical analysis after 30 days, the stem-doubling induction results of the Phalaenopsis plants processed toward an identical orientation and spatially inclined at an angle show that: 71 inducted double stems occur in the Phalaenopsis grandiflora in the group
P7 processed toward an identical orientation and spatially inclined at an angle of 70 to 90 degrees, as 78.9% as a stem-doubling induction rate; 82 inducted double stems occur in the
Phalaenopsis floweret in the group Ps processed toward an identical orientation and spatially inclined at an angle of 70 to 90 degrees, as 91.1% as a stem-doubling induction rate; the
Phalaenopsis grandiflora in the group P7 has 71 double stems more than that in the control group Ps, with the stem-doubling induction rate increased by 78.9%, and the Phalaenopsis floweret in the group Pg has 81 double stems more than that in the control group P4, with the stem-doubling induction rate increased by 90% (see Table 3).
Table 1 stem-doubling induction results cultivated under flower forcing for 30 days
Processi Number Number of Stem-doubling ng NO. Processing method (pots) ducted double induction rate stems (plants) (%)
Py Plant group processed toward an 90 0 0.0 identical orientation (grandiflora)
P2 Plant group processed toward an 90 5 5.6 identical orientation (floweret)
P3 Control group (grandiflora) 90 0 0.0
Ps Control group (floweret) 90 1 1.1
Table 2 stem-doubling induction results cultivated under flower forcing for 30 days
DK 181430 B1 6
Processi Number Number of Stem-doubling ng NO. Processing method (pots) inducted double induction rate stems (plants) (%)
Ps Plant group spatially inclined at an 90 4 4.4 angle (grandiflora)
Ps Plant group spatially inclined at an 90 9 10.0 angle (floweret)
P3 Control group (grandiflora) 90 0 0.0
Ps Control group (floweret) 90 1 1.1
Table 3 stem-doubling induction results cultivated under flower forcing for 30 days
Processi Number Number of Stem-doubling ng NO. Processing method (pots) inducted double induction rate stems (plants) (%)
P+ Plant group processed toward an 90 71 78.9 identical orientation and spatially inclined at an angle (grandiflora)
Ps Plant group processed toward an 90 82 91.1 identical orientation and spatially inclined at an angle (floweret)
P3 Control group (grandiflora) 90 0 0.0
Ps Control group (floweret) 90 1 1.1
It can be seen from Table 3 that regardless of whether the phalaenopsis plants processed toward an identical orientation and spatially inclined at an angle belong to grandiflora or — floweret, they have an improvement in the aspects of the number of inducted double stems and the stem-doubling induction rate. The Phalaenopsis floweret in the group Ps has 82 double stems more than the phalaenopsis grandiflora in the control group Ps with the stem-doubling induction rate increased by 91.1%, 81 double stems more than the phalaenopsis floweret in the group P4 with the stem-doubling induction rate increased by 90%, and 11 double stems more than the phalaenopsis grandiflora in the group Pz with the stem-doubling induction rate increased by 15.4%. The Phalaenopsis grandiflora in the group
P7 has 71 double stems more than the phalaenopsis grandiflora in the control group Ps with the stem-doubling induction rate increased by 78.9%, and 70 double stems more than the phalaenopsis floweret in the group Py with the stem-doubling induction rate increased by 77.8%. It can be seen from the above that the processing toward an identical orientation and
DK 181430 B1 7 the spatial inclination at an angle play an important role in inducing double stems of
Phalaenopsis. 2. Processing of Plants with a Chemical Reagent
The research on the processing of plants with a chemical reagent includes, after completing the step of uniformly adjusting the orientation and spatial angle, spraying the pedicel-inducing mixed solution (TDZ(0.01~0.05g/L)+GA3(0.3~1.0g/L)+IAA(0.1~0.6g/L)+
PP333(0.2~0.8g/L)+P205(0.5~1.2g/L)+glutamic acid(0.8-1.5g/L)) on the stem segments of 2~5 leaves of the Phalaenopsis plants, meanwhile stetting up a control group (without the pedicel-inducing mixed solution sprayed thereon). 30 pots of plants pertain to each — processing, repeatedly trialed 3 times. They are processed with the pedicel-inducing mixed solution once every 10 days to 3 times in total. The spraying method includes using a 150ml small sprinkler each time, and adjusting its mouth to the best state of forming mists applied in one cycle around the stem segments of 2~5 leaves of the Phalaenopsis plants, with stress on spraying the leaf axils of the plants, after spraying, it is suitable that the water mist adheres to the surface of the stem segments and does not flow. The trial results show that the stem-doubling induction rate of the plants sprayed with the pedicel-inducing mixed solution is 98.9% for floweret, 88.9% for grandiflora, so the stem-doubling induction rate of floweret is 10% higher than that of grandiflora, and the average stem-doubling induction rate of floweret and grandiflora is 93.9%,but the stem-doubling induction rate of the plants without — the pedicel-inducing mixed solution spayed thereon is quite low. The detailed trial results are shown in Table 4, wherein,
Po-P10 involve the pedicel-inducing mixed solution A: TDZ(0.03g/L)+GA3(0.6g/L) +IAA(0.4g/L)+P,05(0.5~1.2g/L)+glutamic acid(1.2g/L),
P11-P12 involve the pedicel-inducing mixed solution B: TDZ(0.03g/L)+GA:(0.69/L) +IAA(0.4g/L)+PP333(0.5g/L)+P205(0.9g/L);
P13-P14 involve the pedicel-inducing mixed solution C: TDZ(0.03g/L)+GA3(0.6g/L) +IAA(0.4g/L)+PP333(0.5g/L)+P>05(0.9g/L) + glutamic acid (1.2g/L),
P15-P1s involve the pedicel-inducing mixed solution D: TDZ(0.03g/L)+GA3(0.6g/L) +IAA(0.4g/L) +P20s(0.99/L).
Table 4 Comparison on different Phalaenopsis plants with the pedicel-inducing mixed
DK 181430 B1 8 solution sprayed thereon
Proce Solution conbination Number | Number | Inducti | Pedicel | Pedicel ssing (mg'L') (plants) | of double | onrate | length | diamete
NO. stems (%) (cm) r (cm) (plants)
Plants sprayed with the 72 10.9 0.41 pedicel-inducing mixed solution A (grandiflora)
Pio Plants sprayed with the 81 0.30 pedicel-inducing mixed solution A (floweret)
Pu Plants sprayed with the 73 81.1 92 0.45 pedicel-inducing mixed solution B (grandiflora)
Pr Plants sprayed with the 82 91.1 7.7 0.35 pedicel-inducing mixed solution B (floweret)
Pi3 Plants sprayed with the 88.9 12.8 0.52 pedicel-inducing mixed solution C (grandiflora)
Pua Plants sprayed with the 98.9 10.5 0.38 pedicel-inducing mixed solution C (floweret)
Pis Plants sprayed with the 70 77.8 7.9 0.39 pedicel-inducing mixed solution D (grandiflora)
Pis Plants sprayed with the 88.9 0.25 pedicel-inducing mixed solution D (floweret)
Ps Control group (without the mixed solution sprayed thereon) (grandiflora)
P4 Control group (without the mixed 1 1.1 5.5 0.23 ee | | | 1 17
According to the statistical analysis after 30 days, the result of spraying the pedicel-inducing mixed solution A on the Phalaenopsis plants after completing the step of uniformly adjusting the orientation and spatial angle shows that: the number of the inducted
DK 181430 B1 9 double stems of the Phalaenopsis grandiflora in the group Ps is 72, as 80% as a stem-doubling induction rate, the pedicel length is 10.9cm and the pedicel diameter is 0.41cm; the number of the inducted double stems of the Phalaenopsis floweret in the group Pio is 81, as 90% as a stem-doubling induction rate, the pedicel length is 8.6cm and the pedicel diameter is 0.31cm.
According to the statistical analysis after 30 days, the result of spraying the pedicel-inducing mixed solution B on the Phalaenopsis plant after completing the step of uniformly adjusting the orientation and spatial angle shows that: the number of the inducted double stems of the Phalaenopsis grandiflora in the group Pu is 73, as 81.1% as a stem-doubling induction rate, the pedicel length is 9.2cm and the pedicel diameter is 0.45cm; — the number of the inducted double stems of the Phalaenopsis floweret in the group P12 is 82, as 91.1% as a stem-doubling induction rate, the pedicel length is 7.7cm and the pedicel diameter is 0.35cm.
According to the statistical analysis after 30 days, the result of spraying the pedicel-inducing mixed solution C on the Phalaenopsis plant after completing the step of uniformly adjusting the orientation and spatial angle shows that: the number of the inducted double stems of the Phalaenopsis floweret in the group P14 is 89, as 98.9% as a stem-doubling induction rate, the pedicel length is 10.5cm and the pedicel diameter is 0.38 cm (see FIGs.3, 6 and 7), the Phalaenopsis grandiflora in the group P13 comes second, the number of its inducted double stems is 80, as 88.9% as a stem-doubling induction rate, the pedicel length is 12.8cm and the pedicel diameter is 0.52cm (see FIGs. 4, 5 and 8).
According to the statistical analysis after 30 days, the result of spraying the pedicel-inducing mixed solution D on the Phalaenopsis plant after completing the step of uniformly adjusting the orientation and spatial angle shows that: the number of the inducted double stems of the Phalaenopsis grandiflora in the group Pis is 70, as 77.8% as a stem-doubling induction rate, the pedicel length is 7.9cm and the pedicel diameter is 0.39cm; the number of the inducted double stems of the Phalaenopsis floweret in the group Pisis 80, as 88.9% as a stem-doubling induction rate, the pedicel length is 6.6cm and the pedicel diameter is 0.25cm.
It can be seen from Table 4 that: after completing the step of uniformly adjusting the orientation and spatial angle, the effect of spraying the pedicel-inducing mixed solutions (TDZ(0.01-0.05g/L)+GA:(0.3-1.0g/L)+IAA(0.1—0.6g/L)+PP333(0.2-0.8g/L)+P20s(0.5-1.2g
DK 181430 B1 10 /L)t+glutamic acid (0.8~1.5g/L)) on the Phalaenopsis plants is the best in the aspect of whether the number of the induced double stems or the improvement of the induction rate.
Among them, the Phalaenopsis floweret in the group P14 has the best induction effect with the maximum number of 89 inducted double stems, and the induction rate up to 98.9%, the pedicel length is 10.5cm and the pedicel diameter is 0.38cm (see FIGs.3, 6 and 7). The
Phalaenopsis grandiflora in the group P13 comes second, the number of its inducted double stems is 80, as 88.9% as a stem-doubling induction rate, the pedicel length is 12.8cm and the pedicel diameter is 0.52cm (see FIGs.4, 5 and 8). Compared with P3,P4,P9,P10,P11,P12,P13,P15 and Pic, the number of the inducted double stems of the Phalaenopsis floweret in the group
Pus rises by 89, 88 as a rate of increase as 8800%, 17 as a rate of increase as 23.6%, 8 as a rate of increase as 9.9%, 16 as a rate of increase as 21.9%, 7 as a rate of increase as 8.5%, 9 as a rate of increase as 11.3%, 19 as a rate of increase as 24.1% and 9 as a rate of increase as 11.3%, respectively; its stem-doubling induction rate increases by 98.9%, 97.8%, 18.9%, 8.9%, 17.8%, 7.8%, 10.0%, 21.1% and 10.0%, respectively; its pedicel length rises by 10.5cm, Scm as a rate of increase as 91%, -0.4cm as a rate of decrease as 3.7%, 1.9cm as a rate of increase as 22.1%, 1.3cm as a rate of increase as 14.1%, 2.8cm as a rate of increase as 36.4%, -2.3cm as a rate of decrease as 18.0%, 2.6cm as a rate of increase as 32.9% and 3.9cm as a rate of increase as 59.1%, respectively; its pedicel diameter rises by 0.38cm, 0.15cm as a rate of increase as 65.2%, -0.03cm as a rate of decrease as 7.3%, 0.08cm as a rate of increase as 26.7%, -0.07cm as a rate of decrease as 15.6%, 0.03cm as a rate of increase as 8.6%, -0.14cm as a rate of decrease as 26.9%, -0.01cm as a rate of decrease as 2.6% and 0.13cm as a rate of increase as 52%, respectively. It can be seen from the above that the slight partial disadvantage of the floweret in comparison with the grandiflora in the aspects of the pedicel length and pedicel diameter depends on the characteristics of the varieties themselves; but the — floweret has an absolute advantage in the aspects of the key indexes, the number of inducted double stems and the stem-doubling induction rate. Compared — with
P3,P4,P9,P10,P1,P12,P13,P1s and Pie, the number of the inducted double stems of the
Phalaenopsis grandiflora in the group P13 rises by 80, 79 as a rate of increase as 7900%, 8 as a rate of increase as 11.1%, -1 as a rate of decrease as 1.2%, 7 as a rate of increase as 9.6%, -2 as a rate of decrease as 2.4%, -9 as a rate of decrease as 10.1%, 10 as a rate of increase as 143% and 0 as a rate of increase as 0.0%, respectively; its stem-doubling induction rate
DK 181430 B1 11 increases by 88.9%, 87.8%, 8.9%, -1.1%, 7.8%, -2.2%, -10.0%, 11.1% and 0.0%, respectively; its pedicel length rises by 12.8cm, 7.3cm as a rate of increase as 132.7%, 1.9cm as a rate of increase as 17.4%, 4.2cm as a rate of increase as 48.8%, 3.6cm as a rate of increase as 39.1%, 5.1cm as a rate of increase as 66.2%, 2.3cm as a rate of increase as 21.9%, 4.9cm as a rate of increase as 62.0% and 6.2cm as a rate of increase as 93.9%, respectively; its pedicel diameter rises by 0.52cm, 0.29cm as a rate of increase as 126.1%, 0.11cm as a rate of increase as 26.8%, 0.22cm as a rate of increase as 73.3%, 0.07cm as a rate of increase as 15.6%, 0.17cm as a rate of increase as 48.6%, 0.14cm as a rate of increase as 36.8%, 0.13cm as a rate of increase as 33.3% and 0.27cm as a rate of increase as 108.0%, respectively. It can be seen from the above that the grandiflora has an obvious disadvantage in comparison with the floweret in the aspects of the number of inducted double stems and the stem-doubling induction rate, but has an advantage in the aspects of the pedicel length and the pedicel diameter.
In summary, the method of applying the pedicel-inducing mixed solutions after completing the step of uniformly adjusting the orientation and spatial angle plays a crucial role in the induction of double stems of Phalaenopsis, not only achieving a "zero" breakthrough (Phalaenopsis grandiflora), but also significantly increasing the number of the inducted double stems and raising the stem-doubling induction rate. In addition, the method also has an obvious advantage in increasing the pedicel length and pedicel diameter as a solid — technical foundation in the aspects of cultivating high-yield, high-quality and efficient phalaenopsis and beautifying environments for people’s yearning life.

Claims (3)

DK 181430 B1 12 PATENTKR AVDK 181430 B1 12 PATENTKR AV 1. Fremgangsmåde til højeffektiv frembringelse af dobbelte stængler hos Phalaenopsis, hvor fremgangsmåden omfatter følgende trin:1. Method for highly efficient production of double stems in Phalaenopsis, wherein the method comprises the following steps: S1. fysisk behandling af planter: ensartet justering af det fysiske plan og den rumlige vinkel af — Phalaenopsis-planter, der skal behandles med henblik på blomstermanipulation; ogS1. physical treatment of plants: uniform adjustment of the physical plane and spatial angle of — Phalaenopsis plants to be treated for floral manipulation; and S2. behandling med et kemisk reagens: efter afslutning af S1, sprøjtning af en pedicel- inducerende blandet opløsning på blade og stængelsegmenter af de nævnte Phalaenopsis-planter; afsluttende med at gøre det muligt at inducere dobbelte stængler af Phalaenopsis højeffektivt, hvor trinnet med at justere det fysiske plan i S1 omfatter at arrangere alle Phalaenopsis-planter — ien identisk retning med vækstretningen af stænglerne og bladene på de nævnte Phalaenopsis-planter mod nord og deres rødder mod syd; kendetegnet ved, at i S1 omfatter trinnet med at justere den rumlige vinkel at hæve hældningen af alle de Phalaenopsis-planter, der skal behandles med henblik på blomstermanipulation, til en identisk vinkel for at gøre det muligt for lys at ramme bladakserne af stængelsegmenterne på 3-5 blade af de nævnte Phalaenopsis-planter fra top til bund, og efter at have opelsket planterne 10-20 cm til pediceler under blomstermanipulation i 30 til 40 dage, at fjerne hældningen og vende tilbage til arrangementer på 0 eller 180 grader; i S2 omfatter den pedicel-inducerende blandede opløsning TDZ (0,01-0,05 g/1)+GA; (0,3-1,0 g/l)+ TAA (0,1- 0,6 g/1)+PP333 (0,2-0,8 g/1)+P20 (0,5-1,2 g/1)+ glutaminsyre (0,8-1,5 g/L).S2. treatment with a chemical reagent: after completion of S1, spraying a pedicel-inducing mixed solution on leaves and stem segments of said Phalaenopsis plants; concluding with making it possible to induce double stems of Phalaenopsis highly efficiently, where the step of adjusting the physical plane in S1 includes arranging all Phalaenopsis plants — in an identical direction to the direction of growth of the stems and leaves of said Phalaenopsis plants to the north and their roots to the south; characterized in that in S1 the step of adjusting the spatial angle comprises raising the inclination of all the Phalaenopsis plants to be treated for flower manipulation to an identical angle to allow light to strike the leaf axils of the stem segments of 3 -5 leaves of the said Phalaenopsis plants from top to bottom, and after lifting the plants 10-20 cm to pedicels under flower manipulation for 30 to 40 days, to remove the tilt and return to arrangements of 0 or 180 degrees; in S2, the pedicel-inducing mixed solution comprises TDZ (0.01-0.05 g/1)+GA; (0.3-1.0 g/l)+ TAA (0.1- 0.6 g/1)+PP333 (0.2-0.8 g/1)+P20 (0.5-1.2 g/1)+ glutamic acid (0.8-1.5 g/L). 2. Fremgangsmåde til højeffektivt at inducere dobbelte stængler af Phalaenopsis ifølge krav 1, kendetegnet ved, at sprøjtemetoden i S2 omfatter sprøjtning i en cyklus omkring stængelsegmenterne på 2-5 blade af de nævnte Phalaenopsis-planter ved at bruge en lille sprinkler hver gang, hvilket sikrer, at bladakserne på de nævnte Phalaenopsis-planter sprøjtes, således at vandtågen efter sprøjtning — klæber til overfladen af stængelsegmenterne og ikke flyder.2. A method for highly efficiently inducing double stems of Phalaenopsis according to claim 1, characterized in that the spraying method in S2 comprises spraying in a cycle around the stem segments of 2-5 leaves of said Phalaenopsis plants using a small sprinkler each time, which ensures that the leaf axils of the said Phalaenopsis plants are sprayed so that the water mist after spraying — sticks to the surface of the stem segments and does not flow. 3. Fremgangsmåde til højeffektivt at inducere dobbelte stængler af Phalaenopsis ifølge krav 1, kendetegnet ved, at i S2 påsprøjtes den pedicel-inducerende blandede opløsning en gang hver 9.-12. dag 2-4 gange i alt.3. Method for highly efficiently inducing double stems of Phalaenopsis according to claim 1, characterized in that in S2 the pedicel-inducing mixed solution is sprayed once every 9-12. day 2-4 times in total.
DKPA202270523A 2021-06-28 2022-10-28 Method for high-efficiently inducing double stems of phalaenopsis DK181430B1 (en)

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