CN109122184B - Dendrobium autumn flower bud induction method - Google Patents
Dendrobium autumn flower bud induction method Download PDFInfo
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- CN109122184B CN109122184B CN201811108440.3A CN201811108440A CN109122184B CN 109122184 B CN109122184 B CN 109122184B CN 201811108440 A CN201811108440 A CN 201811108440A CN 109122184 B CN109122184 B CN 109122184B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/60—Flowers; Ornamental plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Abstract
The invention belongs to the field of plant cultivation, and relates to a method for inducing flower buds of dendrobium autumns in a flowering phase, wherein the field planting time of plantlets is selected according to the growth phase of dendrobium autumns, plants with plant heights of more than 20cm and more than 4 pseudo bulbs are selected at a time interval with higher temperature for high-phosphate fertilizer management, and the flower buds of dendrobium autumns are induced by isopentenyl adenine 60 days before the target flowering phase of dendrobium autumns. The method adopts fertilizer management and plant growth regulator to induce the flower buds, has the characteristics of simplicity, convenience, low cost, easiness in operation, high flower bud induction consistency rate and the like, solves the problems that the individual flowering time of the dendrobium nobile in the natural flowering phase is inconsistent, the flower buds cannot be generated neatly and consistently, the dendrobium nobile cannot be delivered in large batches before the target flowering phase, and the dendrobium nobile can be sold on the market on time in a large scale, is favorable for promoting the large-scale production of the dendrobium nobile in tropical orchids, promotes the improvement of the flower quality, and has sustainable development significance for the development of the tropical orchid industry and scientific research work.
Description
Technical Field
The invention belongs to the field of plant cultivation, and relates to a method for inducing plant flower buds, in particular to a method for inducing flower buds of dendrobium officinale.
Background
Dendrobii (Dendrobium spp.) is also called dendrobii, a perennial epiphytic herbaceous plant, is a general name of plants in the genus dendrobii (Dendrobium Swartz, abbreviated as Den) of the family Orchidaceae (Orchidaceae), and is called as "four-large cymbidium" in combination with phalaenopsis, cattleya and cymbidium.
The dendrobium nobile is a group of dendrobium nobile, is evergreen dendrobium nobile and mainly refers to a dendrobium nobile group, a dendrobium antelope group and intercross hybrid of the dendrobium nobile group and the dendrobium nobile group. Mainly distributed in tropical regions in south China, southeast Asia countries and Pacific island countries. The dendrobium nobile can bloom only when the dendrobium nobile prefers a high-temperature and humid environment and grows nutritionally to a certain degree and meets a certain accumulated temperature, and the dendrobium nobile is easy to suffer from freeze injury and even die when the temperature is lower than 12-15 ℃ in winter, and is a typical tropical orchid. The inflorescences of the dendrobium nobile are extracted from the tops of stems, 10-20 flowers are planted in each inflorescence, most of the inflorescences are concentrated in autumn, the natural flowering season is 8-11 months, and the inflorescences are relatively concentrated in 9-10 months. Because of beautiful flower shape, various varieties and long cut flower vase, the cut flower vase is not only an important cut flower variety in orchidaceae, but also an important ornamental pot flower, and is considered as the most promising commercial variety of orchids after the phalaenopsis and the cymbidium hybridum. However, the natural flowering phase of dendrobium nobile is not coincident with the important festivals of the vigorous market demand, such as national festivals, New year's day, spring festival and the like, and the flowering is not regular, so that the situation that flowers exist in the market or flowers exist in the market occurs, the market price greatly fluctuates, and the development of dendrobium nobile production is severely restricted.
Generally, the dendrobium officinale needs a growth period of approximately 10-12 months from a small seedling to a commodity flowering state, through full vegetative growth in a seedling stage, plants gradually tend to mature in a large seedling stage (more than 4 pseudobulbs and more than 20cm in plant height), under appropriate conditions of temperature, illumination, moisture and the like, the newly-extracted pseudobulbs of the dendrobium officinale grow flower buds, but the flowering time is not too concentrated, the dendrobium officinale planted in the same batch often continuously opens within 1-2 months due to individual difference, and the flowering consistency is not very strong. In spring and winter, the temperature is low, so that the formation of flower buds is not facilitated, and the flower does not blossom or less blooms generally.
The flower formation mechanism of dendrobium nobile is not clear, and the measures of utilizing the light cycle and temperature regulation and control have high cost and no obvious effect, so that the method is a technical difficulty in flower bud induction of dendrobium nobile. Nowadays, producers can only sell plants which flower naturally in a flower garden, but cannot produce and sell the plants in batches according to the needs of specific time, and annual supply cannot be realized, so that flowering control of dendrobium nobile is a problem which needs to be solved urgently in production.
At present, the flowering of dendrobium nobile is mainly realized by arranging the production period of dendrobium nobile seedlings and strengthening fertilizer management, the dendrobium nobile flowers essentially independently after being matured, and the flowering time is not very accurate. Even if the same batch of dendrobium nobile is used, because a plurality of individuals have differences in development, a large number of plants cannot bloom or are opened in advance in a target flowering period, and generally, the number of plants which can meet normal delivery is only 40-50%. And landslide of required quantity often appears after the holiday in China, so that the plants opened after the target flowering phase cannot be sold in time, products are accumulated in the circulation link, and the production cost is increased. Moreover, in natural conditions, the inflorescences of natural flowers of dendrobium nobile have a large proportion of less than 8 buds in each inflorescence (generally, more than 12 buds on the inflorescences are regarded as excellent in commercial property), and the quality cannot be guaranteed.
Disclosure of Invention
The invention aims to provide a dendrobium autumn flower bud induction method aiming at the defects of the prior art, adopts fertilizer management combined with a plant growth regulator to induce flower buds, can solve the technical problem that dendrobium autumn flowers are inconsistent, enables the same batch of dendrobium autumn to uniformly extract flower buds and realize batch production, and has the characteristics of simplicity, convenience, low cost, high flower bud induction consistency rate, easiness in operation and the like.
The technical scheme adopted by the invention is as follows:
a method for inducing flower buds of dendrobium officinale in a flowering phase comprises the following specific steps:
1. management of vegetative phase
Selecting a seedling planting time according to the growth period of the dendrobium nobile, and after planting for 9-10 months, selecting plants with plant heights of more than 20cm and more than 4 pseudo bulbs at a time interval with higher temperature (generally planting in 7-10 months of the previous year, namely 5-8 months of the second year) to perform high phosphate fertilizer management (in the high-temperature season, the high-temperature season is very suitable for the growth of the dendrobium nobile, and the pseudo bulbs newly extracted from the base part at the stage are flowering stems). Fertilizing for 4 times per month, wherein the concentration is 0.02%, the pH value is 6.5-7.0, the fertilizing mode is that foliage spraying is combined with root irrigation, and the fertilizing amount of the root irrigation is that a fertilizer solution permeates the culture medium and just can drip out from the bottom. The fertilizer is a nitrogen-phosphorus-potassium balanced fertilizer, namely the proportion of nitrogen, phosphorus and potassium in the fertilizer is N: p2O5：K2O20: 20: 20, administered 3 times per month; in addition, 1 time of high-phosphorus fertilizer application is carried out every month, namely the proportion of nitrogen, phosphorus and potassium in the fertilizer is N: p2O5：K2O ═ 9: 45: 15, promoting the maturity of the pseudobulb of dendrobium autumnale. The water content was controlled to 1 spray every morning and evening. The illumination intensity is controlled to be 7-9 kallux. The management lasts for 1-2 months.
2. Flower bud induction
And (3) spraying leaf surfaces and irrigating roots of the dendrobium nobile seedlings by adopting isopentene adenine [ N6- (2-Isopentenyl) adenosine or 2-iP ] 60 days before the target flowering phase of the dendrobium nobile. The treatment frequency is 4-5 days and 1 time, the total times of use is 5 times, the 1 st use concentration is 50mg/L, the 2 nd use concentration is 100mg/L, the 3 rd use concentration is 150mg/L, the 4 th use concentration is 100mg/L, and the 5 th use concentration is 50 mg/L. 10ml of the fertilizer is sprayed on the leaf surface of each plant and 20ml of the fertilizer is irrigated to the root of each plant, and 30ml of the fertilizer is applied to each plant.
The use of plant growth regulators is necessary to achieve a certain growth rate in the plant and to meet certain environmental conditions. The plant growth amount is at least 3-4 pseudo bulbs, the plant height is not less than 20cm, and the newly-extracted pseudo bulbs are about 10-15 cm in height. The environmental condition requires that the temperature reaches the optimum temperature of 28-35 ℃, and the illumination condition reaches 7-9 ten thousand lux. The optimal effect can be achieved only by adjusting and controlling the flowering phase when the conditions are met.
2-iP is a plant growth regulator, belongs to cytokinin, has the functions of regulating protein synthesis, enzyme activity and cell metabolic balance, stimulating plant cell division, and promoting cell division and differentiation and growth and development of active growth parts. According to the invention, trace 2-iP is adopted to adjust the balance of endogenous hormones of dendrobium nobile, then medium-amount and high-amount 2-iP are used for flower bud induction, and finally the 2-iP amount is reduced to adjust flower bud development, so that the flower bud extraction effect is improved, and the bud burning rate is reduced.
The invention adopts fertilizer management and combines with plant growth regulator to induce flower buds, and has the characteristics of simplicity, convenience, low cost, easy operation, high flower bud induction consistency rate and the like. The method solves the problems that the flowering time of the dendrobium nobile in the natural flowering phase is inconsistent, the flowering buds cannot be generated neatly and consistently, the dendrobium nobile cannot be delivered in large batches before the target flowering phase, and the dendrobium nobile is sold on the market in a large scale on time, is beneficial to promoting the large-scale production of the dendrobium nobile in tropical orchids, promotes the investment of flower enterprises in tropical regions in China on the production of the dendrobium nobile, forms large-scale industrialization, and promotes the improvement of flower quality, thereby having sustainable development significance for the development of the tropical orchid industry and scientific research work.
Detailed Description
The following examples are given to further illustrate the embodiments of the present invention. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention. The experimental methods in the following examples, which are not specified under specific conditions, are generally performed under conventional conditions.
Example one
The target florescence is selected as the festival of national celebration, namely, the target florescence is November and one day. Selecting a tissue culture seedling of a well-grown and robust dendrobium nobile variety Malaysia Green (Den. 'Malaysia Green') to be planted in a flowerpot in the last 10 months of the year, and starting to implement treatment in the 8 months of the year. Selecting 300 plants with plant height of more than 20cm and more than 4 pseudobulbs, setting 5 management areas, wherein each management area comprises 60 plants, the number of the treatment areas is 3, namely a treatment area A, a treatment area B and a treatment area C, the number of the comparison areas is 2, namely a comparison area I and a comparison area II.
The nutrient period management measures are as follows:
the dendrobium officinale plantlets grow to be more than 20cm in plant height and have more than 4 pseudobulbs, and the pseudobulbs newly extracted from the base parts at the stage are flowering stems. The production management mode of plants in the treatment area A, B, C and the control area I is consistent, fertilization is carried out for 4 times per month, the concentration is 0.02%, the pH value is 6.5-7.0, the fertilization mode is root irrigation, and the fertilization amount is that fertilizer liquid permeates the culture medium and just drips out from the bottom. The fertilizer is nitrogen-phosphorus-potassium balanced fertilizer (N: P)2O5：K2O20: 20: 20) 3 times per month, and 1 time per month of high phosphate fertilizer (N: p2O5：K2O ═ 9: 45: 15) to promote the maturity of the pseudobulb of dendrobium officinale. And the control area II adopts nitrogen-phosphorus-potassium balanced fertilizer and does not apply high-phosphorus fertilizer. The water content was controlled to 1 spray every morning and evening. The illumination intensity is controlled to be about 8 ten thousand lux.
The specific measures of flower bud induction treatment are as follows:
and (3) treatment area A: flower bud induction treatment is carried out on Malaysia green 60 days before the target flowering phase. Spraying leaves and irrigating roots of the dendrobium officinale by using 2-iP, wherein 10ml of leaves are sprayed on each plant, and 20ml of leaves are used for irrigating the roots. The root irrigation is carried out when the substrate is basically dry, and the liquid medicine is fully permeated into the culture substrate and is preferably just dripped out of the bottom of the nutrition cup. After treatment, water can not be sprayed in the same day to avoid the loss of liquid medicine. The treatment is carried out once every 4 days for a total of 5 times, with the 1 st use concentration being 50mg/L, the 2 nd use concentration being 100mg/L, the 3 rd use concentration being 150mg/L, the 4 th use concentration being 100mg/L and the 5 th use concentration being 50 mg/L.
And (4) managing water, spraying for 1 time in the morning and at night every day, reducing the spraying amount one day before the flower forcing treatment, properly drying the culture substrate, and allowing the liquid medicine to permeate, wherein the spraying can not be carried out again the day after the treatment. The temperature is kept at 28-35 ℃, and the illumination intensity is controlled at 6-8 ten thousand lux.
And a treatment area B: flower bud induction treatment is carried out on Malaysia green 60 days before the target flowering phase. Spraying leaves and irrigating roots of the dendrobium officinale by using 2-iP, wherein 10ml of leaves are sprayed on each plant, and 20ml of leaves are used for irrigating the roots. The treatment was performed every 7 days for a total of 3 times, with a 1 st use concentration of 200mg/L, a 2 nd use concentration of 200mg/L, and a 3 rd use concentration of 200 mg/L. Moisture, temperature and light management are the same as treatment area a.
And a treatment area C: flower bud induction treatment is carried out on Malaysia green 60 days before the target flowering phase. Spraying leaves and irrigating roots of the dendrobium officinale by using 2-iP, wherein 10ml of leaves are sprayed on each plant, and 20ml of leaves are used for irrigating the roots. The treatment was carried out once every 7 days for a total of 3 times, with the 1 st application concentration being 100mg/L, the 2 nd application concentration being 100mg/L and the 3 rd application concentration being 100 mg/L. Moisture, temperature and light management are the same as treatment area a.
Control zone i, control zone ii: the Malaysia green was treated with clear water 60 days before the target flowering phase. Each plant was sprayed with 10ml of clean water on leaves and 20ml of clean water was used for root irrigation. The root irrigation is carried out when the substrate is basically dry, so that the moisture can fully permeate the culture substrate and can just drip out of the bottom of the nutrition cup. The rinsing treatment was performed every 7 days for a total of 3 times. Water content management, spraying 1 time each day in the morning and evening. The temperature is kept at 28-35 ℃, and the illumination intensity is controlled at 6-8 ten thousand lux.
Example two
The target florescence is selected as the festival of national celebration, namely, the target florescence is November and one day. Tissue culture seedlings of a good and robust dendrobium nobile variety 'Hongxiang' (Den. 'Nopporn pink') are selected to be planted in flowerpots in the last 10 months of the year, and the treatment is started in the current 7 months of the year. Selecting 300 plants with plant height of more than 20cm and more than 4 pseudobulbs, setting 5 management areas, wherein each management area comprises 60 plants, the number of the treatment areas is 3, namely a treatment area A, a treatment area B and a treatment area C, the number of the comparison areas is 2, namely a comparison area I and a comparison area II. The nutrient phase management and flower bud induction treatment of each management area are the same as those of the first embodiment.
Flower bud extraction and flower morphology identification:
and determining that the flower bud is extracted when the length of the flower bud reaches 1cm, and judging that the flower bud induction is successful.
Observing the flower bud extraction condition and the bud burning condition in the flower bud development process of the plants in the management areas in the above embodiments, counting the flower bud extraction number, and calculating the flower bud induction rate and the bud burning rate, wherein the results are as follows:
and (3) treatment area A:
the Malaysia green starts to generate flower buds after being induced and treated for 20 days, the flower buds of plants which grow 8 days later than 40 days after treatment all generate flower buds, and the flower bud drawing rate reaches 93.7 percent after 50 days, and the increase is small. The number of the flower buds extracted by 60 days is 57 on average, and the flower bud extraction rate is 95.0%. After all flower buds bloom, the number of buds is 18.2 flowers/inflorescence on average, the number of buds burnt is 1.2 flowers/inflorescence on average finally, and the bud burning rate is 6.6%.
"Hongxiang" began to develop flower buds 23 days after induction treatment, and flower buds appeared in 8 or more plants 40 days after treatment, after which the flower extraction rate increased to 91.6% at day 50. The number of flower buds extracted is 55.4 by day 60, and the flower bud extraction rate is 92.3%. After all flower buds bloom, the number of buds is 16.5 flowers/inflorescence on average, the number of buds burned is 1.3 on average in the final statistics, and the bud burning rate is 7.8%.
And a treatment area B:
the Malaysia green starts to generate flower buds after 20 days of induction treatment, and the flower bud drawing rate exceeds 8 and 9 after 50 days after 30 days of treatment. The number of flower buds extracted is 59 by day 60, and the flower bud extraction rate is 98.3%. After all flower buds bloom, the number of buds is 18.8 flowers/inflorescence on average, the number of buds burnt is 3.6 flowers/inflorescence on average finally, and the bud burning rate is 19.1%.
"Hongxiang" began to develop flower buds 21 days after induction treatment, nearly 8 plants developed flower buds 30 days after treatment, and the flower extraction rate increased to 95.0% on day 50, after which there was little change. The number of flower buds extracted is 58 by day 60, and the flower bud extraction rate is 96.7%. After all flower buds bloom, the number of buds is averagely 17.4 buds/inflorescence, the number of buds burned is finally counted to be 3.9 buds/inflorescence, and the bud burning rate is 22.4%.
And a treatment area C:
the Malaysia green starts to generate flower buds 22 days after induction treatment, the plants which grow beyond 4 days after the treatment day 40 have flower buds, and the increase is not large after the plants reach 66.7 percent after the treatment day 50. The number of flower buds extracted by 60 days is 43, and the flower bud extraction rate is 71.2%. After all flower buds bloom, the number of buds is 16.5 flowers/inflorescence on average, the bud burning rate is 0.5 flowers/inflorescence finally counted, and the bud burning rate is 3.0%.
"Hongxiang" began to develop flower buds 25 days after induction treatment, nearly 4 plants developed flower buds 40 days after treatment, and the flower extraction rate increased to 58.3% at day 50, after which there was little change. The number of flower buds extracted is 39 by day 60, and the flower bud extraction rate is 65.0%. After all flower buds bloom, the average number of buds is 14.3 buds/inflorescence, the number of buds burned is finally counted to be 0.7 buds/inflorescence, and the bud burning rate is 4.9%.
Control zone i:
flower buds appear 33 days after the 'malaysia green' treated by clear water, 16.8 flower buds are extracted from the 'malaysia green' to the 60 th day, and the flower bud extraction rate is 28.0%. The average number of buds is 10.8, the number of buds burned is finally counted as 0.1 buds/inflorescence, and the bud burning rate is 0.9%.
The flower buds appear 28 days after the clean water treatment of the "Hongxian" and 22 flower buds are extracted from the "Hongxian" to the 60 th day, the extraction rate of the flower buds is 36.7 percent and is slightly higher than that of the "Malaysia green". The average number of buds is 11.6 buds/inflorescence, the number of buds burned is finally counted as 0 buds/inflorescence, and the bud burning rate is 0%.
Control zone ii:
flower buds appear after 40 days of 'malaysia green' treated by clear water, the number of the flower buds is 14.5 from 60 days, and the flower bud extraction rate is 24.2%. The average number of buds is 10.2 buds/inflorescence, the final statistics of the number of buds burned is 0.1 buds/inflorescence, and the bud burning rate is 0.1%.
Flower buds appear 35 days after the clean water treatment of the Hongxiang, 18.4 flower buds are extracted from the date 60, and the flower bud extraction rate is 30.7%. The average number of buds is 10.5 buds/inflorescence, the number of buds burned is finally counted as 0 buds/inflorescence, and the bud burning rate is 0%.
And (4) analyzing results:
the control zone treated with clear water can be considered as a condition of natural flowering. In the target flowering phase, the final flowering bud rate of Malaysia green is less than 30%, namely more than 70% of the autumn dendrobium can not bloom on time; the flowering rate of the "Hongxiang" is slightly higher, which reaches 30% or more, but more than one and a half of plants still can not flower on time. In contrast, when high-phosphate fertilizer is applied in the vegetative stage, the flower bud extraction rate of dendrobium officinale is higher than that of plants without high-phosphate fertilizer.
The flower bud drawing rate of the dendrobium nobile subjected to the 2-iP flower bud induction treatment is higher. At the target flowering stage, although the "malaysia green" and "rosmarinus" final flower bud rates of 98.3% and 96.7% were the highest in all treatments in treatment zone B, the bud burn rates were also highest, reaching 19.1% and 22.4%, respectively; although the abnormal flower bud burning rates of Malaysia green and Hongyxia in the treatment area C are lower, namely 3.0% and 4.9%, the flower bud drawing rates are obviously lower, namely 71.2% and 65.0%; the final flower-drawing bud rates of Malaysia green and Hongxia in the treatment area A reach 95.0% and 92.3% respectively, the flower-drawing bud rates are not obviously different from the result of the treatment area B, meanwhile, the bud burning rates are 6.6% and 7.8%, the bud burning rates are far lower than that of the treatment area B, the market acceptable degree is reached, and the flower-drawing bud rate-reducing treatment area A can be applied to large-scale and industrial production of commercial flowers.
In conclusion, flower buds subjected to flower forcing treatment in the treatment area A are drawn out regularly and are obviously higher than the result of clear water treatment, the bud burning rate is relatively low, and the requirement of batch shipment is met. The method can effectively and orderly induce and extract flower buds.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the technical principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (2)
1. A method for inducing flower buds of dendrobium officinale in a flowering phase is characterized by comprising the following specific steps:
1) management of the vegetative stage
Selecting the field planting time of the plantlets according to the growth period of the dendrobium officinale, and selecting plants with plant heights of more than 20cm and more than 4 pseudo bulbs to perform high-phosphate fertilizer management in a time period with higher temperature after field planting for 9-10 months: fertilizing for 4 times per month, wherein the concentration is 0.02%, the pH value is 6.5-7.0, the fertilizing mode is that foliage spraying is combined with root irrigation, and the fertilizing amount of the root irrigation is that a fertilizer solution permeates the culture medium and just can drip out from the bottom; the fertilizer is a nitrogen-phosphorus-potassium balanced fertilizer, namely the proportion of nitrogen, phosphorus and potassium in the fertilizer is N: p2O5：K2O20: 20: 20, administered 3 times per month; in addition, 1 time of high-phosphorus fertilizer application is carried out every month, namely the proportion of nitrogen, phosphorus and potassium in the fertilizer is N: p2O5：K2O ═ 9: 45: 15; the water content is managed to be sprayed for 1 time in the morning and evening every day; the illumination intensity is controlled to be 7-9 million lux;
2) flower bud induction
Spraying and irrigating leaf surfaces and roots of the dendrobium autumn seedlings with isopentene adenine 60 days before the target flowering phase of the dendrobium autumn, wherein the treatment frequency is 4-5 days for 1 time, the total use time is 5 times, the 1 st use concentration is 50mg/L, the 2 nd use concentration is 100mg/L, the 3 rd use concentration is 150mg/L, the 4 th use concentration is 100mg/L, and the 5 th use concentration is 50 mg/L; 10ml of the fertilizer is sprayed on the leaf surface of each plant and 20ml of the fertilizer is irrigated to the root of each plant, and 30ml of the fertilizer is applied to each plant.
2. The method for inducing flowering buds of dendrobium autumbergii in flowering phase according to claim 1, wherein the method comprises the following steps: and during the whole flowering period, the temperature of the growth environment of the dendrobium officinale is 28-35 ℃.
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| CN104620992B (en) * | 2015-03-03 | 2016-08-31 | 佛山市顺德区今日景艺生物科技有限公司 | Herba Urariae Lagopodioidis orchid the induction of the lateral bud culture medium and cultural method |
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