CN102550257A - Method for promoting early flowering of Dendrobium nobile by combination of low temperature and plant growth regulator - Google Patents
Method for promoting early flowering of Dendrobium nobile by combination of low temperature and plant growth regulator Download PDFInfo
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- CN102550257A CN102550257A CN2012100137217A CN201210013721A CN102550257A CN 102550257 A CN102550257 A CN 102550257A CN 2012100137217 A CN2012100137217 A CN 2012100137217A CN 201210013721 A CN201210013721 A CN 201210013721A CN 102550257 A CN102550257 A CN 102550257A
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- 240000004638 Dendrobium nobile Species 0.000 title claims abstract description 79
- 238000000034 method Methods 0.000 title claims abstract description 42
- 239000005648 plant growth regulator Substances 0.000 title claims abstract description 31
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 title abstract description 20
- 230000001737 promoting effect Effects 0.000 title abstract 2
- 238000005507 spraying Methods 0.000 claims abstract description 5
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 claims description 10
- 239000007921 spray Substances 0.000 claims description 10
- HFCYZXMHUIHAQI-UHFFFAOYSA-N Thidiazuron Chemical group C=1C=CC=CC=1NC(=O)NC1=CN=NS1 HFCYZXMHUIHAQI-UHFFFAOYSA-N 0.000 claims description 9
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 claims description 5
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- 241000196324 Embryophyta Species 0.000 abstract description 10
- 230000006698 induction Effects 0.000 abstract description 10
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- 238000012258 culturing Methods 0.000 abstract 2
- 230000000052 comparative effect Effects 0.000 description 6
- 239000005556 hormone Substances 0.000 description 4
- 229940088597 hormone Drugs 0.000 description 4
- 230000033228 biological regulation Effects 0.000 description 3
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- 238000011160 research Methods 0.000 description 3
- JLIDBLDQVAYHNE-LXGGSRJLSA-N 2-cis-abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\C1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-LXGGSRJLSA-N 0.000 description 2
- 241001523681 Dendrobium Species 0.000 description 2
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- 239000000463 material Substances 0.000 description 2
- 231100000572 poisoning Toxicity 0.000 description 2
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- PVRBGBGMDLPYKG-UHFFFAOYSA-N 6-benzyl-7h-purine Chemical compound N=1C=NC=2N=CNC=2C=1CC1=CC=CC=C1 PVRBGBGMDLPYKG-UHFFFAOYSA-N 0.000 description 1
- 241000732800 Cymbidium Species 0.000 description 1
- 241000026010 Dendrobium candidum Species 0.000 description 1
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Abstract
The invention discloses a method for promoting early flowering of Dendrobium nobile by combination of low temperature and plant growth regulators. The method comprises the following steps: culturing Dendrobium nobile for 45 days in an alpine greenhouse, keeping day and night temperatures in the alpine greenhouse at 18-31 DEG C and 7-18 DEG C, respectively, and spraying plant growth regulator solution to Dendrobium nobile stems every 10 days during the culture; after alpine greenhouse cultivation, transferring Dendrobium nobile to a greenhouse in a plain, keeping the day and night temperatures at 25 DEG C and 20 DEG C, respectively, and culturing for 60-70 days till Dendrobium nobile plants flower. The method of the invention can promote flower bud induction and normal flowering for Dendrobium nobile, significantly increase normal flowering rate of plants, normal flowering rate of nodes and average number of flowers per node, thus greatly improving the viewing quality and commercial value of Dendrobium nobile. Compared with alpine flower induction method in prior arts, the method can achieve a better flower induction effect in the aspects of normal flowering rate, flower number per plant and the like, flower quality has been markedly improved, and no harm is caused by the use of plant growth regulators.
Description
Technical field
The invention belongs to orchid florescence control technical field, be specifically related to a kind of method that promotes that the spring stem of noble dendrobium is bloomed in advance, particularly a kind of low temperature and plant growth regulator of combining promotes the method that the spring stem of noble dendrobium is bloomed in advance.
Background technology
Florescence control is meant with manual method control flowers flowering time.The most of spring stem of noble dendrobium (Dendrobium Nobile) crossbreed is induced without high mountain, and 3-5 month is open after the Spring Festival usually, and the orchid of just having missed Chinese New Year's Day, the Spring Festival is in great demand the busy season.
The festive and the seasonality that be to adapt to China's flowers consumption are necessary to regulate florescence of the spring stem of noble dendrobium, make it advance to New Year's Day or bloom the Spring Festival.To the spring stem of noble dendrobium crossbreed carry out flower forcing and handle, be to carry out the key link that its commercialization is produced.
The spring stem of noble dendrobium is bloomed needs a low temperature induction process, and promptly in day 15～20 ℃ of temperature, induce 30～40 day ability formation bud (Huang Dinghua under 10～15 ℃ of the temperature night.Flowers florescence control new technology, Chinese agriculture publishing house, 1999), perhaps short flower temperature is controlled at 15～20 ℃ daytime, at 8～15 ℃ of nights, can form bud in 30～50 days.After bud formed, night, temperature remained on 18～20 ℃, and 6～8 weeks just can bloom.
Spring, stem of noble dendrobium crossbreed went on the market during the Spring Festival, generally need mid or late October to early November carry out flower forcing (time Jingjing, Li Qing. the cultivation of the spring stem of noble dendrobium and propagation technique. south agricultural, 2009,3 (2): 42-44).
Spring stem of noble dendrobium crossbreed florescence control mainly comes regulation florescence through refrigeration or high mountain low temperature at present.Yet the simple high mountain flower forcing technology weather conditions that place one's entire reliance upon bring a series of factors of instability, and, bloom problem such as irregular few like yellow leaf, fallen leaves, bud abortion, the joint number of blooming has a strong impact on the quality and the market competitiveness of its potted flower.Combine the high mountain low temperature treatment through cheap plant growth regulator, can obviously promote the maturation of spring stem of noble dendrobium crossbreed pseudobulb and improve the level of normally blooming, thereby further improve ornamental value and commercial value.
Research shows, hormone is participated in the adjusting and the control of vernalization, with the spring stem of noble dendrobium (Den.pink Beauty ' Queen ' * Den.pink Gem ' Elegance ') (22 ± 1 ℃, 10h)/(10 ± 1 ℃; 14h) handle under (daytime/evening) condition, at the vernalization preinduction period, endogenous hormones GA3 content increases with handling time lengthening; Reach maximum at 30d, ABA reaches the highest at 20d, than high more than 4 times before handling; Show and vernalization promote the accumulation of endogenous hormones GA and ABA (leaf is celebrated one's birthday for Pan Shuqun, Zhuan Junping.The variation of Physiology and biochemistry in the spring stem of noble dendrobium vernalization process. tropical crop journal, 2007,28 (3): 38-41).
The plant growth regulator regulation florescence has been used quite general, induces the test tube flowering of hybrid cymbidium, Dendrobidium huoshanness, dendrobium candidum, Moth orchid tissue cultivating seedling like the 6-benzyl purine.
Plant growth regulator shows spring stem of noble dendrobium land for growing field crops florescence control result of the test, without low temperature induction, October the appliable plant growth regulator can promote spring stem of noble dendrobium crossbreed Den001 before the Spring Festival, to bloom, but the highest joint flowering rate is merely 21.5%; November, the appliable plant growth regulator can promote the big luxuriant sheath stem of noble dendrobium of spring stem of noble dendrobium initial species to shift to an earlier date 20d to bloom, and the joint flowering rate reaches 50%, and flower matter obviously improves (Wang Zaihua, Zhu Genfa, Ou Mengchang, Wang Biqing.Plant growth regulator promotes the effect research of blooming in advance of the spring stem of noble dendrobium. guangdong agricultural science, and 2008,10:37-39).
Summary of the invention
In order to overcome the problem that exists in the existing regulating and controlling blooming period of spring dendrobium stem, the object of the present invention is to provide effective combination low temperature of a kind of flower forcing and plant growth regulator to promote the method that the spring stem of noble dendrobium is bloomed in advance.
The object of the invention is realized through following technical proposals:
A kind of low temperature and plant growth regulator of combining promotes the method that the spring stem of noble dendrobium is bloomed in advance, is divided into two stages of floral induction and flower bud initiation, and its concrete steps are following:
After spring stem of noble dendrobium pseudobulb maturation, be placed in the high mountain greenhouse and cultivated 45 days, the day and night temperature in high mountain greenhouse be respectively 18-31 ℃ with 7-18 ℃, therebetween whenever at a distance from 10 days to the spring stem of noble dendrobium stem spray the plant growth regulator solution of 10-100mg/L; After the high mountain hot-house culture finished, the spring stem of noble dendrobium had grown bud, and move to the spring stem of noble dendrobium in the greenhouse, level land this moment again, and the day and night temperature in greenhouse, level land is respectively 25 ℃ and 20 ℃, cultivates after 60-70 days, and the spring stem of noble dendrobium is promptly bloomed.
The described stem of noble dendrobium crossbreed Den001 of preferred spring of the spring stem of noble dendrobium or Den2810;
Preferred Thidiazuron of described plant growth regulator or abscisic acid;
The amount of spraying of said plant growth regulator solution is 5-15 milliliter/strain;
In the said high mountain hot-house culture process, the day and night temperature in greenhouse of preceding half stage (referring to preceding 20-25 days) is respectively 27～31 ℃ and 14～18 ℃; The day and night temperature in rear half stage greenhouse is respectively 18～25 ℃ and 7～10 ℃;
In the said high mountain hot-house culture process, every separated two weeks imposes the fertile solution of flower forcing to spring stem of noble dendrobium root system, and uses CaNO
3Solution, MgSO
4Solution sprays in spring stem of noble dendrobium blade face;
Fertile preferred the spending the more of described flower forcing, wherein N, P
2O
5, K
2The mass ratio of O is 10: 30: 20, and the concentration of the fertile solution of flower forcing is 500mg/L;
Said Ca (NO
3)
2The concentration of solution is 200mg/L, said MgSO
4The concentration of solution is 125mg/L.
Among the present invention, the chemical name of Thidiazuron (TDZ) is 1-phenyl-3-(1,2; 3-thiadiazoles-5-yl) urea, the chemical name of abscisic acid (ABA) are 3-methyl-5-(1 '-hydroxyl-4 '-oxygen-2 ', 6 '; 6 '-trimethyl-2 '-cyclohexene-1 '-yl)-2, the 4-pentadienoic acid.
The principle of the invention is: regularly uses Thidiazuron (TDZ) or abscisic acid (ABA), promotes the plant pseudobulb ripe, regulate making a start of bud and grow thereby regulate Endogenous Hormones, and then effective regulation florescence.
The present invention has following advantage and effect with respect to prior art:
The present invention adopts plant growth regulator to combine the method for low temperature treatment; Can obviously promote inducing and opening of spring stem of noble dendrobium bud, improve the normal flowering rate of strain, the normal flowering rate of joint significantly and save a number of on average blooming, compare with control group (only low temperature treatment); Saving normal flowering rate improves more than 2 times; Thereby improved the ornamental quality and the commercial value of the spring stem of noble dendrobium greatly, and reagent cost is cheap, easy and simple to handle.With existing high mountain flower-forcing method relatively, the inventive method aspect such as a number of can blooming in normal flowering rate, strain reaches better flower forcing effect, the quality of flower is improved significantly, and does not produce the poisoning because of using plant growth regulator to cause.
Description of drawings
Fig. 1 is the spring stem of noble dendrobium that the method for method and the Comparative Examples 1 of embodiment 1 the is handled effect contrast figure of blooming.
Fig. 2 is the spring stem of noble dendrobium that the method for method and the Comparative Examples 2 of embodiment 2 the is handled effect contrast figure of blooming.
Embodiment
Below in conjunction with embodiment and accompanying drawing the present invention is described in further detail, but embodiment of the present invention is not limited thereto.
Embodiment 1
A kind of low temperature and plant growth regulator of combining promotes the method that the spring stem of noble dendrobium is bloomed in advance, may further comprise the steps:
(1) the ripe plant of spring stem of noble dendrobium crossbreed Den001 is (available from Chinese Academy of Sciences South China Botanical Garden; About 30 centimetres of plant height, about 2 years of vegetative period), treat its pseudobulb maturation after; Be placed in the high mountain greenhouse and cultivated 45 days, the day and night temperature in greenhouse is respectively 18.5-29.8 ℃ and 7.0-17.3 ℃.During the high mountain hot-house culture, every at a distance from 10 days to the spring stem of noble dendrobium stem spray the Thidiazuron solution of 10mg/L, the amount of spraying is 5-15 milliliter/strain, every at a distance from two weeks with 500mg/L flower flower forcing fertilizer solution (N: P the more
2O
5: K
2O=10: 30: 20) irritates root, 200mg/LCa (NO
3)
2Solution or 125mg/L MgSO
4Solution sprays spring stem of noble dendrobium blade face.After the high mountain hot-house culture finished, the spring stem of noble dendrobium had grown bud.
(2) the spring stem of noble dendrobium is moved in the greenhouse, level land, the day and night temperature in greenhouse is respectively 25 ℃ and 20 ℃, cultivates after 60 days, and the spring stem of noble dendrobium is promptly bloomed.
Embodiment 2
(1) the ripe plant of spring stem of noble dendrobium crossbreed Den2810 (Dendrobium Frigdaas Pink Star; Guangdong Province flowers research institute of research of agricultural science institute is on sale; About 50 centimetres of plant height, about 2 years of vegetative period), treat its pseudobulb maturation after; Be placed in the high mountain greenhouse and cultivated 45 days, the day and night temperature in greenhouse is respectively 18.5-29.8 ℃ and 7.0-17.3 ℃.During the high mountain hot-house culture, every at a distance from 10 days to the spring stem of noble dendrobium stem spray the abscisic acid solution of 10mg/L, the amount of spraying is 5-15 milliliter/strain, every at a distance from two weeks with 500mg/L flower flower forcing fertilizer solution (N: P the more
2O
5: K
2O=10: 30: 20) irritates root, 200mg/L Ca (NO
3)
2Solution or 125mg/LMgSO
4Solution sprays spring stem of noble dendrobium blade face.After the high mountain hot-house culture finished, the spring stem of noble dendrobium had grown bud.
(2) the spring stem of noble dendrobium is moved in the greenhouse, level land, the day and night temperature in greenhouse is respectively 25 ℃ and 20 ℃, cultivates after 63 days, and the spring stem of noble dendrobium is promptly bloomed.
Comparative Examples 1
A kind of method that promotes that the spring stem of noble dendrobium is bloomed in advance may further comprise the steps:
(1) the high mountain hot-house culture does not spray Thidiazuron solution, and other materials, method and condition are with embodiment 1 step (1).
(2) the spring stem of noble dendrobium is moved in the greenhouse, level land, the day and night temperature in greenhouse is respectively 25 ℃ and 20 ℃, cultivates after 66 days, and the spring stem of noble dendrobium is promptly bloomed.
Comparative Examples 2
A kind of method that promotes that the spring stem of noble dendrobium is bloomed in advance may further comprise the steps:
(1) the high mountain hot-house culture does not spray Thidiazuron solution, and other materials, method and condition are with embodiment 2 steps (1).
(2) the spring stem of noble dendrobium is moved in the greenhouse, level land, the day and night temperature in greenhouse is respectively 25 ℃ and 20 ℃, cultivates after 60 days, and the spring stem of noble dendrobium is promptly bloomed.
The method of embodiment 1-2 is to the flower induction and the influence of normally blooming:
Compare with reference examples 1; The method of embodiment 1 is bloomed to spring stem of noble dendrobium crossbreed Den001 and is produced utmost point appreciable impact; Strain flower induction rate, save normal flowering rate, a strain number and the joint number of on average blooming of on average blooming and improve 47.8%, 16.5%, 3.3 and 0.6 (seeing table 1) respectively.Flowering time and Sheng are taken time and are carried about the last week, and the flower footpath does not extremely significantly diminish, and quality is had no adverse effects, and simultaneously, can be known by accompanying drawing 1, and embodiment 1 fallen leaves are few, and flowering is neat, and ornamental value is higher.
The influence that the method for table 1 embodiment 1 is bloomed to Den001
Compare with Comparative Examples 2, the method for embodiment 2 is bloomed to spring stem of noble dendrobium crossbreed Den2810 and is produced utmost point appreciable impact, and strain flower induction rate improves 18.9% and 33.9% (seeing table 2) respectively with the normal flowering rate of joint; Flowering time is not produced utmost point appreciable impact, and the florescence is delayed about a week a little, but still open before the Spring Festival.Can know by accompanying drawing 2, embodiment 2 neat appearance of blooming, the joint number of blooming extremely significantly increases, and ornamental value is higher.
The influence that the method for table 2 embodiment 2 is bloomed to Den2810
Annotate: table 1-2 is all in statistics in March next year, and statistics plant number is 10 strain/processing, repeats 3 times, and every strain statistics is number 1～6 joint reciprocal from the bottom up; Strain flower induction rate: (the strain number/plant number of blooming) * 100%, normal flowering rate: (joint number of blooming/total joint number) * 100%.A strain number of on average blooming: (number/plant number of blooming) * 100%, a joint number of on average blooming: (number of blooming/total joint number) * 100%.Beginning take time and Sheng to take time with the junior one in the first month of the lunar year be the boundary, statistics is fate in advance.
In sum, compare with Comparative Examples, embodiment 1 and embodiment 2 handle through increasing plant growth regulator; Obviously promotion spring stem of noble dendrobium crossbreed normally blooms; And improved the quality of blooming greatly, and do not produce obvious poisoning, should the colored comparatively ideal of commodity in the Spring Festival to producing.
The foregoing description is a preferred implementation of the present invention; But embodiment of the present invention is not restricted to the described embodiments; Other any do not deviate from change, the modification done under spirit of the present invention and the principle, substitutes, combination, simplify; All should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (7)
1. one kind combines low temperature and plant growth regulator to promote the method that the spring stem of noble dendrobium is bloomed in advance, it is characterized in that may further comprise the steps:
After spring stem of noble dendrobium pseudobulb maturation, be placed in the high mountain greenhouse and cultivated 45 days, the day and night temperature in high mountain greenhouse be respectively 18-31 ℃ with 7-18 ℃, therebetween whenever at a distance from 10 days to the spring stem of noble dendrobium stem spray the plant growth regulator solution of 10-100mg/L; The high mountain hot-house culture moves to the spring stem of noble dendrobium in the greenhouse, level land after finishing, and the day and night temperature in greenhouse, level land is respectively 25 ℃ and 20 ℃, cultivates after 60-70 days, and the spring stem of noble dendrobium is promptly bloomed.
2. combination low temperature according to claim 1 and plant growth regulator promote the method that the spring stem of noble dendrobium is bloomed in advance, and it is characterized in that: the described spring stem of noble dendrobium is spring stem of noble dendrobium crossbreed Den001 or Den2810.
3. combination low temperature according to claim 1 and plant growth regulator promote the method that the spring stem of noble dendrobium is bloomed in advance, and it is characterized in that: described plant growth regulator is Thidiazuron or abscisic acid.
4. combination low temperature according to claim 1 and plant growth regulator promote the method that the spring stem of noble dendrobium is bloomed in advance, and it is characterized in that: the amount of spraying of said plant growth regulator solution is 5-15 milliliter/strain.
5. combination low temperature according to claim 1 and plant growth regulator promote the method that the spring stem of noble dendrobium is bloomed in advance, it is characterized in that: in the described high mountain hot-house culture process, every separated two weeks imposes the fertile solution of flower forcing to spring stem of noble dendrobium root system, and uses CaNO
3Solution, MgSO
4Solution sprays in spring stem of noble dendrobium blade face.
6. combination low temperature according to claim 5 and plant growth regulator promote the method that the spring stem of noble dendrobium is bloomed in advance, it is characterized in that:
The concentration of the fertile solution of said flower forcing is 500mg/L;
Described flower forcing is fertile in spending the more, wherein N, P
2O
5, K
2The mass ratio of O is 10: 30: 20.
7. combination low temperature according to claim 5 and plant growth regulator promote the method that the spring stem of noble dendrobium is bloomed in advance, it is characterized in that: said Ca (NO
3)
2The concentration of solution is 200mg/L, said MgSO
4The concentration of solution is 125mg/L.
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Cited By (5)
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| CN103477845A (en) * | 2013-09-23 | 2014-01-01 | 东莞市粮作花卉研究所 | Dendrobium refrigerator flower forcing method |
| CN105453925A (en) * | 2015-12-14 | 2016-04-06 | 广东省农业科学院环境园艺研究所 | Method for regulating flowering phase of cymbidium ensifolium |
| CN109315259A (en) * | 2018-11-01 | 2019-02-12 | 广西壮族自治区林业科学研究院 | A kind of flower induction method of nobile-type dendrobium seedling |
| CN110692482A (en) * | 2019-10-21 | 2020-01-17 | 浙江省萧山棉麻研究所 | Forcing culture method for Lisui pineapple |
| CN113016390A (en) * | 2021-03-23 | 2021-06-25 | 上海市农业科学院 | Method for promoting loquat terminal bud to flower |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103477845A (en) * | 2013-09-23 | 2014-01-01 | 东莞市粮作花卉研究所 | Dendrobium refrigerator flower forcing method |
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| CN109315259A (en) * | 2018-11-01 | 2019-02-12 | 广西壮族自治区林业科学研究院 | A kind of flower induction method of nobile-type dendrobium seedling |
| CN110692482A (en) * | 2019-10-21 | 2020-01-17 | 浙江省萧山棉麻研究所 | Forcing culture method for Lisui pineapple |
| CN113016390A (en) * | 2021-03-23 | 2021-06-25 | 上海市农业科学院 | Method for promoting loquat terminal bud to flower |
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