CN102550257B - Method for promoting early flowering of Dendrobium nobile by combination of low temperature and plant growth regulator - Google Patents
Method for promoting early flowering of Dendrobium nobile by combination of low temperature and plant growth regulator Download PDFInfo
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- CN102550257B CN102550257B CN 201210013721 CN201210013721A CN102550257B CN 102550257 B CN102550257 B CN 102550257B CN 201210013721 CN201210013721 CN 201210013721 CN 201210013721 A CN201210013721 A CN 201210013721A CN 102550257 B CN102550257 B CN 102550257B
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- 240000004638 Dendrobium nobile Species 0.000 title claims abstract description 79
- 238000000034 method Methods 0.000 title claims abstract description 44
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Abstract
The invention discloses a method for promoting early flowering of Dendrobium nobile by combination of low temperature and plant growth regulators. The method comprises the following steps: culturing Dendrobium nobile for 45 days in an alpine greenhouse, keeping day and night temperatures in the alpine greenhouse at 18-31 DEG C and 7-18 DEG C, respectively, and spraying plant growth regulator solution to Dendrobium nobile stems every 10 days during the culture; after alpine greenhouse cultivation, transferring Dendrobium nobile to a greenhouse in a plain, keeping the day and night temperatures at 25 DEG C and 20 DEG C, respectively, and culturing for 60-70 days till Dendrobium nobile plants flower. The method of the invention can promote flower bud induction and normal flowering for Dendrobium nobile, significantly increase normal flowering rate of plants, normal flowering rate of nodes and average number of flowers per node, thus greatly improving the viewing quality and commercial value of Dendrobium nobile. Compared with alpine flower induction method in prior arts, the method can achieve a better flower induction effect in the aspects of normal flowering rate, flower number per plant and the like, flower quality has been markedly improved, and no harm is caused by the use of plant growth regulators.
Description
Technical field
The invention belongs to orchid florescence control technical field, be specifically related to a kind of method that promotes spring stem of noble dendrobium Blooming, particularly a kind of method in conjunction with low temperature and plant growth regulator promotion spring stem of noble dendrobium Blooming.
Background technology
Florescence control refers to control by artificial means the flowers flowering time.The most of spring stem of noble dendrobium (Dendrobium Nobile) crossbreed is induced without high mountain, and 3-5 month is open after the Spring Festival usually, and the orchid of just having missed Chinese New Year's Day, the Spring Festival is in great demand the busy season.
For adapting to property in red-letter day and the seasonality of China's flowers consumption, be necessary to regulate the florescence of the spring stem of noble dendrobium, make it advance to New Year's Day or bloom the Spring Festival.To the spring stem of noble dendrobium crossbreed carry out flower forcing and process, be to carry out the key link that its commercialization is produced.
The spring stem of noble dendrobium is bloomed needs a low temperature induction process, and namely in day 15～20 ℃ of temperature, induce 30～40 day ability to form bud (Huang Dinghua under 10～15 ℃ of temperature night.Flowers florescence control new technology, Chinese agriculture publishing house, 1999), perhaps short flower temperature is controlled at 15～20 ℃ daytime, at 8～15 ℃ of nights, can form bud in 30～50 days.After bud formed, night, temperature remained on 18～20 ℃, and 6～8 weeks just can bloom.
Spring, stem of noble dendrobium crossbreed went on the market at Spring Festival, generally need to mid or late October to early November carry out flower forcing (time Jingjing, Li Qing. the cultivation of the spring stem of noble dendrobium and propagation technique. agriculture in south, 2009,3 (2): 42-44).
Spring stem of noble dendrobium crossbreed florescence control mainly comes regulation florescence by refrigeration or high mountain low temperature at present.Yet the simple high mountain flower forcing technology weather conditions that place one's entire reliance upon bring a series of factors of instability, and as few in yellow leaf, fallen leaves, bud abortion, the joint number of blooming, the problem such as irregular of blooming has a strong impact on quality and the market competitiveness of its potted flower., can obviously promote the maturation of spring stem of noble dendrobium crossbreed pseudobulb and improve the level of normally blooming, thereby further improving ornamental value and commercial value in conjunction with the high mountain low temperature treatment by cheap plant growth regulator.
Studies show that, hormone participates in the regulation and control of vernalization, with the spring stem of noble dendrobium (Den.pink Beauty'Queen' * Den.pink Gem'Elegance') at (22 ± 1 ℃, 10h)/(10 ± 1 ℃, 14h) process under (daytime/evening) condition, at the vernalization preinduction period, endogenous hormones GA3 content increases with processing time lengthening, reach maximum at 30d, ABA reaches the highest at 20d, than high more than 4 times before processing, shows that vernalization promotes the accumulation (Pan Shuqun of endogenous hormones GA and ABA, Zhuan Junping, leaf is celebrated one's birthday.The variation of Physiology and biochemistry in spring stem of noble dendrobium vernalization process. tropical crop journal, 2007,28 (3): 38-41).
The plant growth regulator regulation florescence has been used quite general, induces the test tube flowering of hybrid cymbidium, Dendrobidium huoshanness, dendrobium candidum, Moth orchid group training seedling as the 6-benzyl purine.
Plant growth regulator shows spring stem of noble dendrobium land for growing field crops florescence control result of the test, without low temperature induction, October the appliable plant growth regulator can promote spring stem of noble dendrobium crossbreed Den001 to bloom before the Spring Festival, but the highest joint flowering rate is only 21.5%; November, the appliable plant growth regulator can promote the large luxuriant sheath stem of noble dendrobium of spring stem of noble dendrobium initial species to shift to an earlier date 20d to bloom, and the joint flowering rate reaches 50%, and flower matter obviously improves (Wang Zaihua, Zhu Genfa, Ou Mengchang, Wang Biqing.Plant growth regulator promotes spring stem of noble dendrobium Blooming effect research. guangdong agricultural science, and 2008,10:37-39).
Summary of the invention
In order to overcome the problem that exists in existing regulating and controlling blooming period of spring dendrobium stem, the object of the present invention is to provide a kind of effective method in conjunction with low temperature and plant growth regulator promotion spring stem of noble dendrobium Blooming of flower forcing.
Purpose of the present invention is achieved through the following technical solutions:
A kind of method in conjunction with low temperature and plant growth regulator promotion spring stem of noble dendrobium Blooming is divided into two stages of floral induction and flower bud initiation, and its concrete steps are as follows:
After spring stem of noble dendrobium pseudobulb maturation, be placed in the high mountain greenhouse and cultivated 45 days, the day and night temperature in high mountain greenhouse is respectively 18-31 ℃ and 7-18 ℃, therebetween every 10 days to the spring stem of noble dendrobium stem spray the plant growth regulator solution of 10-100mg/L; After the high mountain hot-house culture finished, the spring stem of noble dendrobium had grown bud, and move to the spring stem of noble dendrobium in the greenhouse, level land this moment again, and the day and night temperature in greenhouse, level land is respectively 25 ℃ and 20 ℃, cultivates after 60-70 days, and the spring stem of noble dendrobium is namely bloomed.
The described stem of noble dendrobium crossbreed Den001 of preferred spring of the spring stem of noble dendrobium or Den2810;
The preferred Thidiazuron of described plant growth regulator or abscisic acid;
The amount of spraying of described plant growth regulator solution is 5-15 milliliter/strain;
In described high mountain hot-house culture process, first half stage (referring to front 20-25 days) the day and night temperature in greenhouse is respectively 27～31 ℃ and 14～18 ℃; The day and night temperature in rear half stage greenhouse is respectively 18～25 ℃ and 7～10 ℃;
In described high mountain hot-house culture process, every two weeks, spring stem of noble dendrobium root system is imposed the fertile solution of flower forcing, and with Ca (NO
3)
2Solution, MgSO
4Solution sprays in spring stem of noble dendrobium blade face;
Fertile preferred the spending the more of described flower forcing, wherein N, P
2O
5, K
2The mass ratio of O is 10:30:20, and the concentration of the fertile solution of flower forcing is 500mg/L;
Described Ca(NO
3)
2The concentration of solution is 200mg/L, described MgSO
4The concentration of solution is 125mg/L.
In the present invention, the chemical name of Thidiazuron (TDZ) is 1-phenyl-3-(1,2,3-thiadiazoles-5-yl) urea, the chemical name of abscisic acid (ABA) is 3-methyl-5-(1 '-hydroxyl-4 '-oxygen-2 ', 6 ', 6 '-trimethyl-2 '-cyclohexene-1 '-yl)-2, the 4-pentadienoic acid.
The principle of the invention is: regularly uses Thidiazuron (TDZ) or abscisic acid (ABA), promotes the plant pseudobulb ripe, regulate making a start and growing of bud thereby regulate Endogenous Hormones, and then the Effective Regulation florescence.
The present invention has following advantage and effect with respect to prior art:
The present invention adopts plant growth regulator in conjunction with the method for low temperature treatment, can obviously promote inducing and opening of spring stem of noble dendrobium bud, improve significantly the normal flowering rate of strain, the normal flowering rate of joint and save a number of on average blooming, compare with control group (only low temperature treatment), saving normal flowering rate improves more than 2 times, thereby greatly improved ornamental quality and the commercial value of the spring stem of noble dendrobium, and reagent cost is cheap, easy and simple to handle.With existing high mountain flower-forcing method relatively, the inventive method aspect such as a number of can blooming in normal flowering rate, strain reaches better flower forcing effect, the quality of flower is improved significantly, and does not produce the poisoning because using plant growth regulator to cause.
Description of drawings
Fig. 1 is the spring stem of noble dendrobium that the method for the method of embodiment 1 and Comparative Examples 1 the is processed effect contrast figure of blooming.
Fig. 2 is the spring stem of noble dendrobium that the method for the method of embodiment 2 and Comparative Examples 2 the is processed effect contrast figure of blooming.
Embodiment
The present invention is described in further detail below in conjunction with embodiment and accompanying drawing, but embodiments of the present invention are not limited to this.
Embodiment 1
A kind of method in conjunction with low temperature and plant growth regulator promotion spring stem of noble dendrobium Blooming comprises the following steps:
(1) the ripe plant of spring stem of noble dendrobium crossbreed Den001 is (available from Chinese Academy of Sciences South China Botanical Garden, approximately 30 centimetres of plant heights, approximately 2 years vegetative period), after its pseudobulb maturation, be placed in the high mountain greenhouse and cultivated 45 days, the day and night temperature in greenhouse is respectively 18.5-29.8 ℃ and 7.0-17.3 ℃.During the high mountain hot-house culture, every 10 days to the spring stem of noble dendrobium stem spray the Thidiazuron solution of 10mg/L, the amount of spraying is 5-15 milliliter/strain, every two weeks with the 500mg/L flower fertile solution (N:P of flower forcing the more
2O
5: K
2O=10:30:20) fill with root, 200mg/LCa (NO
3)
2Solution or 125mg/L MgSO
4Solution sprays spring stem of noble dendrobium blade face.After the high mountain hot-house culture finished, the spring stem of noble dendrobium had grown bud.
(2) the spring stem of noble dendrobium is moved in the greenhouse, level land, the day and night temperature in greenhouse is respectively 25 ℃ and 20 ℃, cultivates after 60 days, and the spring stem of noble dendrobium is namely bloomed.
Embodiment 2
(1) the ripe plant of spring stem of noble dendrobium crossbreed Den2810 (Dendrobium Frigdaas Pink Star, flowers research institute of Guangdong Agricultural research institute is on sale, approximately 50 centimetres of plant heights, approximately 2 years vegetative period), after its pseudobulb maturation, be placed in the high mountain greenhouse and cultivated 45 days, the day and night temperature in greenhouse is respectively 18.5-29.8 ℃ and 7.0-17.3 ℃.During the high mountain hot-house culture, every 10 days to the spring stem of noble dendrobium stem spray the abscisic acid solution of 10mg/L, the amount of spraying is 5-15 milliliter/strain, every two weeks with the 500mg/L flower fertile solution (N:P of flower forcing the more
2O
5: K
2O=10:30:20) fill with root, 200mg/L Ca (NO
3)
2Solution or 125mg/LMgSO
4Solution sprays spring stem of noble dendrobium blade face.After the high mountain hot-house culture finished, the spring stem of noble dendrobium had grown bud.
(2) the spring stem of noble dendrobium is moved in the greenhouse, level land, the day and night temperature in greenhouse is respectively 25 ℃ and 20 ℃, cultivates after 63 days, and the spring stem of noble dendrobium is namely bloomed.
Comparative Examples 1
A kind of method that promotes spring stem of noble dendrobium Blooming comprises the following steps:
(1) the high mountain hot-house culture does not spray Thidiazuron solution, and other materials, method and condition are with embodiment 1 step (1).
(2) the spring stem of noble dendrobium is moved in the greenhouse, level land, the day and night temperature in greenhouse is respectively 25 ℃ and 20 ℃, cultivates after 66 days, and the spring stem of noble dendrobium is namely bloomed.
Comparative Examples 2
A kind of method that promotes spring stem of noble dendrobium Blooming comprises the following steps:
(1) the high mountain hot-house culture does not spray Thidiazuron solution, and other materials, method and condition are with embodiment 2 steps (1).
(2) the spring stem of noble dendrobium is moved in the greenhouse, level land, the day and night temperature in greenhouse is respectively 25 ℃ and 20 ℃, cultivates after 60 days, and the spring stem of noble dendrobium is namely bloomed.
The method of embodiment 1-2 is on flower induction and the impact of normally blooming:
Compare with reference examples 1, the method of embodiment 1 is bloomed on spring stem of noble dendrobium crossbreed Den001 and is produced utmost point appreciable impact, strain flower induction rate, save normal flowering rate, a strain number and the joint number of on average blooming of on average blooming and improve respectively 47.8%, 16.5%, 3.3 and 0.6 (seeing Table 1).Flowering time and Sheng are taken time and are carried about the last week, and Hua Jing does not extremely significantly diminish, and quality is had no adverse effects, and simultaneously, by accompanying drawing 1 as can be known, embodiment 1 fallen leaves are few, and flowering is neat, and ornamental value is higher.
The impact that the method for table 1 embodiment 1 is bloomed on Den001
Compare with Comparative Examples 2, the method for embodiment 2 is bloomed on spring stem of noble dendrobium crossbreed Den2810 and is produced utmost point appreciable impact, strain flower induction rate and the normal flowering rate of joint improve respectively 18.9% and 33.9%(see Table 2); Flowering time is not produced utmost point appreciable impact, and the florescence is delayed about a week a little, but still open before the Spring Festival.By accompanying drawing 2 as can be known, embodiment 2 neat appearance of blooming, the joint number of blooming extremely significantly increases, and ornamental value is higher.
The impact that the method for table 2 embodiment 2 is bloomed on Den2810
Annotate: table 1-2 is all in statistics in March next year, and statistics plant number is 10 strain/processing, repeats 3 times, and every strain statistics is number 1～6 joint reciprocal from the bottom up; Strain flower induction rate: (Flowering Plants number/plant number) * 100%, normal flowering rate: (joint number of blooming/total joint number) * 100%.A strain number of on average blooming: (number/plant number of blooming) * 100%, a joint number of on average blooming: (number of blooming/total joint number) * 100%.Beginning takes time and Sheng is taken time take the junior one in the first month of the lunar year as the boundary, and statistics is number of days in advance.
In sum, compare with Comparative Examples, embodiment 1 and embodiment 2 are by increasing plant growth regulator treatment, obviously promotion spring stem of noble dendrobium crossbreed normally blooms, and greatly improved Flowering quality, and do not produce obvious poisoning, should the colored comparatively ideal of commodity in the Spring Festival to producing.
Above-described embodiment is the better embodiment of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under Spirit Essence of the present invention and principle, substitutes, combination, simplify; all should be the substitute mode of equivalence, within being included in protection scope of the present invention.
Claims (2)
1. method that promotes spring stem of noble dendrobium Blooming in conjunction with low temperature and plant growth regulator is characterized in that comprising the following steps:
After spring stem of noble dendrobium pseudobulb maturation, be placed in the high mountain greenhouse and cultivated 45 days, the day and night temperature in high mountain greenhouse is respectively 18-31 ℃ and 7-18 ℃, therebetween every 10 days to the spring stem of noble dendrobium stem spray the plant growth regulator solution of 10-100mg/L; The high mountain hot-house culture moves to the spring stem of noble dendrobium in the greenhouse, level land after finishing, and the day and night temperature in greenhouse, level land is respectively 25 ℃ and 20 ℃, cultivates after 60-70 days, and the spring stem of noble dendrobium is namely bloomed;
In described high mountain hot-house culture process, the day and night temperature in first half stage greenhouse is respectively 27 ~ 31 ℃ and 14 ~ 18 ℃; The day and night temperature in rear half stage greenhouse is respectively 18 ~ 25 ℃ and 7 ~ 10 ℃; The described first half stage refers to front 20-25 days.
2. the method that promotes spring stem of noble dendrobium Blooming in conjunction with low temperature and plant growth regulator according to claim 1, it is characterized in that: the described spring stem of noble dendrobium is spring stem of noble dendrobium crossbreed Den001 or Den2810.
3, the method in conjunction with low temperature and plant growth regulator promotion spring stem of noble dendrobium Blooming according to claim 1, it is characterized in that: described plant growth regulator is Thidiazuron or abscisic acid.
4, the method in conjunction with low temperature and plant growth regulator promotion spring stem of noble dendrobium Blooming according to claim 1, it is characterized in that: the amount of spraying of described plant growth regulator solution is 5-15 milliliter/strain.
5, the method that promotes spring stem of noble dendrobium Blooming in conjunction with low temperature and plant growth regulator according to claim 1 is characterized in that: in described high mountain hot-house culture process, every two weeks, spring stem of noble dendrobium root system is imposed the fertile solution of flower forcing, and with Ca (NO
3)
2Solution, MgSO
4Solution sprays in spring stem of noble dendrobium blade face.
6, the method in conjunction with low temperature and plant growth regulator promotion spring stem of noble dendrobium Blooming according to claim 5 is characterized in that:
The concentration of the fertile solution of described flower forcing is 500mg/L;
Described flower forcing is fertile in spending the more, wherein N, P
2O
5, K
2The mass ratio of O is 10:30:20.
7, the method in conjunction with low temperature and plant growth regulator promotion spring stem of noble dendrobium Blooming according to claim 5, is characterized in that: described Ca (NO
3)
2The concentration of solution is 200mg/L, described MgSO
4The concentration of solution is 125mg/L.
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| CN103477845B (en) * | 2013-09-23 | 2014-12-17 | 东莞市粮作花卉研究所 | Dendrobium refrigerator flower forcing method |
| CN105453925B (en) * | 2015-12-14 | 2018-05-15 | 广东省农业科学院环境园艺研究所 | A kind of method at regulation and control sword-leaved cymbidium florescence |
| CN109315259A (en) * | 2018-11-01 | 2019-02-12 | 广西壮族自治区林业科学研究院 | A kind of flower induction method of nobile-type dendrobium seedling |
| CN110692482A (en) * | 2019-10-21 | 2020-01-17 | 浙江省萧山棉麻研究所 | Forcing culture method for Lisui pineapple |
| CN113016390A (en) * | 2021-03-23 | 2021-06-25 | 上海市农业科学院 | Method for promoting loquat terminal bud to flower |
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| CN1225964C (en) * | 2004-01-08 | 2005-11-09 | 华南农业大学 | Spring dendrobe flowering period control method |
| CN100431404C (en) * | 2005-11-02 | 2008-11-12 | 中国林业科学研究院林业研究所 | Method for regulating and controlling blooming period of spring dendrobium stem |
| CN101156535B (en) * | 2006-09-27 | 2010-12-01 | 中国林业科学研究院林业研究所 | Method for controlling flowering and propagation of dendrobium nobile |
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Address after: 510640 No. 1 East five street, Tianhe District mountain road, Guangdong, Guangzhou, China Patentee after: ENVIRONMENTAL HORTICULTURE Research Institute OF GUANGDONG ACADEMY OF AGRICULTURAL SCIENCES Address before: 510640 No. 1 East five street, Tianhe District mountain road, Guangdong, Guangzhou, China Patentee before: Floriculture Research Institute of Guangdong Academy of Agricultural Sciences |
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Granted publication date: 20130626 |