GB2613254A - Method for efficiently inducing double stems of Phalaenopsis SSP - Google Patents

Method for efficiently inducing double stems of Phalaenopsis SSP Download PDF

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GB2613254A
GB2613254A GB2216951.0A GB202216951A GB2613254A GB 2613254 A GB2613254 A GB 2613254A GB 202216951 A GB202216951 A GB 202216951A GB 2613254 A GB2613254 A GB 2613254A
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phalaenopsis
plants
pedicel
stem
rate
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GB202216951D0 (en
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Huang Guiyun
Zhang Haibo
Wu Di
Wu Jinhua
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China Three Gorges Corp
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China Three Gorges Corp
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
    • A01H3/04—Processes for modifying phenotypes, e.g. symbiosis with bacteria by treatment with chemicals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/02—Flowers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/04—Stems
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/62—Orchidaceae [Orchid family]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
    • A01N43/647—Triazoles; Hydrogenated triazoles
    • A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/26—Phosphorus; Compounds thereof

Abstract

The present invention provides a method for efficiently inducing double stems of Phalaenopsis spp.: after treating Phalaenopsis spp. by means of a chemical agent treatment combination (0.01 to 0.05 g/L TDZ + 0.3 to 1.0 g/L GA3 + 0.1 to 0.6 g/L IAA + 0.2 to 0.8 g/L PP333 + 0.5 to 1.2 g/L P2O5 + 0.8 to 1.5 g/L glutamic acid), double stems may be quickly and efficiently induced. The method is suitable for the industrialized flower promotion of Phalaenopsis spp. and has low production costs, and the obtained Phalaenopsis spp. has high quality and good consistency.

Description

Description
METHOD FOR EFFICIENTLY INDUCING DOUBLE STEMS OF
PHALAENOPSIS SPP
FIELD OF THE INVENTION
The present invention relates to the technical field of plant handling, in particular to a method for high-efficiently inducing double stems of Phalaenopsis.
BACKGROUND OF THE INVENTION
Phalaenopsis is a Phalaenopsis genus plant in the Orchid family, known as the "Queen of Foreign Orchid" with peculiar flower shape, gorgeous color and long-lasting florescence The article "Investigating the spiking and double spiking ratio of Phalaenopsis within the air-inflated double-poly envelope inside a plastic greenhouse" published by Liu Yaoren said that after more than 30 days of flower forcing the stem-doubling induction rates of different Phalaenopsis varieties were 50% of Sunset Red, 27% of Lawrence, 17% of V3, and only 3% of Jubao Red Rose. The article "Why Phalaenopsis is the king of New Year's eve flowers" carried by "China Flower & Gardening News" at the end of March this year said that the amount of the Phalaenopsis put on the market during the New Year's eve in 2020 in China is estimated to exceed 55 million, and the total sales amount is expected to be more than 1 billion yuan, which is the sum of the sales amounts of several other major varieties ranked behind In the aspect of double-stem phalaenopsis varieties, Europe has a great advantage over China, as European breeders have cultivated double-stem varieties for more than 20 years. For middle and small flower shapes, double-stem varieties are more economical for growers than single-stem varieties. For distribution customers in China, while the buying price of single-stein Phalaenopsis products is 20 yuan, the buying price of high-quality double-stem products is generally 25 yuan at a maximum premium of 25%. Thus, it can be seen that it is of important practical significance to perfect the research of double-stem Phalaenopsis for improving people's life quality and developing China into a beautiful country.
SUMMARY OF THE INVENTION
In order to solve the above-mentioned technical problems, the present invention provides a method for high-efficiently inducing double stems of Phalaenopsis, which enables people to enjoy the sight of cultivating double-stem varieties, but greatly saves the time and costs required for
Description
breeding.
The technical solution of the present invention is as follows.
A method for high-efficiently inducing double stems of Phalaenopsis includes the following steps: St. physical processing of plants: uniformly adjusting the physical plane and spatial angle of Phalaenopsis plants to be processed for flower forcing; and S2. processing with a chemical reagent after completing Si, spraying a pedicel-inducing mixed solution on the stem segments of 2-5 leaves of the Phalaenopsis plants once every 10 days to 3 times in total; ending with enabling double stems of Phalaenopsis to be high-efficiently induced.
Preferably, in Sl, the step of adjusting the physical plane includes arranging all Phalaenopsis plants toward an identical orientation with the growth direction of the stems and leaves of plants toward north and their roots toward south.
Preferably, in Si, the step of adjusting the spatial angle includes raising the inclination of all the Phalaenopsis plants to be processed for flower forcing to an identical angle, so as to incline the plants toward the bottom surface of a swayed bed at an angle of 70 to 90 degrees and then enable light to strike the leaf axils of the stem segments of 3-5 leaves of the Phalaenopsis plants from top to bottom, after growing to 10-20cm for pedicels under flower forcing for 30 to 40 days, removing the inclination and returning to arrangements at 0 or 180 degrees.
Preferably, in S2, the pedicel-inducing mixed solution includes TDZ (0.01-0.050i+ GA3(0.3-1. Og/L)+1AA(0.1-0.6g/L)+PP333(0.2-0.8g/L)+P205(0.5-1.2g/L)+ glutamic acid (0.8-1.5g/L).
Preferably, in S2, the spraying method includes using a 150m1 small sprinkler each time, and adjusting its mouth to the best state of forming mists applied in one cycle around the stem segments of 2-5 leaves of the Phalaenopsis plants, with stress on spraying the leaf axils of the plants, after spraying, it is suitable that the water mists adhere to the surface of the stem segments and does not flow.
Preferably, in S2, the pedicel-inducing mixed solution is applied once every 9-12 days to 2-4 times in total.
The present invention has beneficial effects as follows.
1 Plants can be processed simply and effectively outdoor, and the double stems of Phalaenopsis can be induced periodically.
Description
2.The adjustment of the physical plane of the Phalaenopsis plants can not only make earth's magnetic field pass through the plant bodies smoothly, but also minimize the impact of the magnetic field on the plant, facilitating the operations such as fertilizing, watering and changing pots.
3.The adjustment of the physical spatial angle of the Phalaenopsis plants helps light to strike on the leaf axils of the stern segments of 3-5 leaves of the Phalaenopsis plants from top to bottom, promoting the germination of double stems.
4.The chemical processing can effectively boost the plants to change form vegetative growth to reproductive growth, and promote flower-bud differentiation and double stern rate, so it is of great significance in large-scale production.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG.] shows the consistent orientation of the planes of the Phalaenopsis leaves.
FIG. 2 shows the processing of the spatial inclination to the Phalaenopsis plants.
FIG.3 shows the induction of the double stems of the Phalaenopsis plant group.
FIG.4 shows the induction of the double stems of the single Phalaenopsis plant.
FIG.5 shows the flowering plant of the double-stem Phalaenopsis in the color system of Red (Red Sun).
FIG.6 shows the flowering plant of the double-stem Phalaenopsis in the color system of Yell ow (Richness-Happiness Sunset).
FIG.7 shows the flowering plant of the double-stem Phalaenopsis in the color system of Orange (Golden Butterfly).
FIGS shows the flowering plant of the double-stem Phalaenopsis in the color system of Red (Century Red).
DETAILED DESCRIPTION OF SOME EMBODIMENTS
In order to make the objective, technical solution and advantages of the present invention clearer, we will further detail the present invention in combination with the examples as follows, however, it should be understood that the specific examples described herein are only used to explain the present invention, but not to pose any limitation on the present invention.
Example 1
I. Physical Processing of Plants: In order to promote the germination of double stems smoothly and effectively, we physically process the Phalaenopsis plants in advance, that is, uniformly adjust the physical plane and spatial
Description
angle of the Phalaenopsis plants to be processed for flower forcing. Firstly, the step of adjusting the physical plane includes arranging all Phalaenopsis plants toward an identical orientation, this trial greenhouse is in the north-south orientation(other similar greenhouses can be adjusted according to the actual situation), which orientates the growth direction of the stems and leaves of the plants toward north and its roots toward south, not only guaranteeing the growth of the plants along the earth's magnetic field and the minimization of the impact of the magnetic field on the plant, but also facilitating the operations such as fertilizing, watering and changing pots (see FIG.1). Secondly, the step of arranging all Phalaenopsis plants toward an identical orientation is followed by the step of adjusting the spatial angle, which includes raising the inclination of all the Phalaenopsis plants to be processed for flower forcing to an identical angle, so as to incline the plants toward the bottom surface of a swayed bed at an angle of 70 to 90 degrees and then enable light to strike the leaf axils of the stem segments of I-5 leaves of the Phalaenopsis plants from top to bottom, after growing to 10-20cm for pedicels under flower forcing for 30 to 40 days, removing the inclination and returning to the arrangement at 0 or 180 degrees (see FIG.2). The detailed experimental results are shown in Tables 1-3.
According to the statistical analysis after 30 days, the stem-doubling induction results of the Phalaenopsis plants processed toward an identical orientation show that: no inducted double stem occurs in the Phalaenopsis grandiflora in the group Pi only processed toward an identical orientation, as 0% as a stem-doubling induction rate; 5 inducted double stems occur in the Phalaenopsis floweret in the group P2 processed toward an identical orientation, as 5.6% as a stem-doubling induction rate; no inducted double stem still occurs in the Phalaenopsis grandiflora in the control group P3, as 0% as a stem-doubling induction rate; and 1 inducted double stem occurs in the Phalaenopsis tloweret in the control group P4, as 1.1% as a stem-doubling induction rate (see Table 1).
According to the statistical analysis after 30 days, the stem-doubling induction results of the Phalaenopsis plants spatially inclined at an angle show that: 4 inducted double stems occur in the Phalaenopsis grandiflora in the group P5 only spatially inclined at an angle of 70 to 90 degrees, as 4.4% as a stem-doubling induction rate; 9 inducted double stems occur in the Phalaenopsis floweret in the group P6 spatially inclined at an angle of 70 to 90 degrees, as 10.0% as a stern-doubling induction rate; the Phalaenopsis grandiflora in the group P5 has 4 double stems more than that in the control group P3, with the stem-doubling induction rate increased by 4.4%, and the Phalaenopsis
Description
floweret in the group P6 has 8 double stems more than that in the control group P4, with the stem-doubling induction rate increased by 8.9% (see Table 2).
According to the statistical analysis after 30 days, the stem-doubling induction results of the Phalaenopsis plants processed toward an identical orientation and spatially inclined at an angle show that: 71 inducted double stems occur in the Phalaenopsis grandiflora in the group P7 processed toward an identical orientation and spatially inclined at an angle of 70 to 90 degrees, as 78.9% as a stem-doubling induction rate; 82 inducted double stems occur in the Phalaenopsis floweret in the group P8 processed toward an identical orientation and spatially inclined at an angle of 70 to 90 degrees, as 91.1% as a stem-doubling induction rate; the Phalaenopsis grandiflora in the group P7 has '71 double sterns more than that in the control group P3, with the stern-doubling induction rate increased by 78.9%, and the Phalaenopsis floweret in the group P8 has 81 double stems more than that in the control group P4, with the stem-doubling induction rate increased by 90% (see Table 3).
Table 1 stem-doubling induction results cultivated under flower forcing for 30 days Proccssi Processing method Number (pots) Number of Stem-doubling ng NO. inducted double induction ratc stems (plants) (%) P1 Plant group processed toward an identical orientation (grandi flora) 90 0 0.0 Plant group processed toward an identical orientation (floweret) 90 5 5.6 Pl. Control group (grandiflora) 90 0 0.0 P4 Control group (floweret) 90 1 1.1 Table 2 stem-doubling induction results cultivated under flower forcing for 30 days Processi Number Number of Stem-doubling lig NO. Processing method (pots) inducted double induction rate stems (plants) (A) P5 Plant group spatially inclined at an angle (grandiflora) 90 4 4.4 P6 Plant group spatially inclined at an angle (floweret) 90 9 10.0 P3 Control group (grandiflora) 90 0 0.0 P4 Control group (floweret) 90 1 1.1
Description
Table 3 stem-doubling induction results cultivated under flower forcing for 30 days Processi Number Number of Stem-doubling ng NO. Processing method (pots) inducted double induction rate stems (plants) (%) P7 Plant group processed toward an 90 71 78.9 identical orientation and spatially inclined at an angle (grandiflora) Pg Plant group processed toward an 90 82 91.1 identical orientation and spatially inclined at an angle (floweret) P3 Control group (grandillora) 90 0 0.0 P4 Control group (floweret) 90 1 1.1 It can be seen from Table 3 that regardless of whether the phalaenopsis plants processed toward an identical orientation and spatially inclined at an angle belong to grandiflora or floweret, they have an improvement in the aspects of the number of inducted double stems and the stem-doubling induction rate. The Phalaenopsis floweret in the group Ps has 82 double stems more than the phalaenopsis grandiflora in the control group P3 with the stem-doubling induction rate increased by 91.1%, 81 double sterns more than the phalaenopsis floweret in the group P4 with the stem-doubling induction rate increased by 90%, and 11 double stems more than the phalaenopsis grandiflora in the group P7 with the stem-doubling induction rate increased by 15.4%. The Phalaenopsis grandiflora in the group P7 has 71 double stems more than the phalaenopsis grandiflora in the control group P3 with the stem-doubling induction rate increased by 78.9%, and 70 double stems more than the phalaenopsis floweret in the group P4 with the stem-doubling induction rate increased by 77.8%. It can be seen from the above that the processing toward an identical orientation and the spatial inclination at an angle play an important role in inducing double stems of Phalaenopsis.
2. Processing of Plants with a Chemical Reagent The research on the processing of plants with a chemical reagent includes, after completing the step of uniformly adjusting the orientation and spatial angle, spraying the pedicel-inducing mixed solution (TDZ(0.01-0.05g1)+0A3(0.3-1.0g/L)+1.AA(0.1-0.6g/L)+ PP333(0.2-0.8g/L)+P203(0.5-1.2g/L)+glutamic acid(0.8-1.5g/L)) on the stem segments of 2-5 leaves of the Phalaenopsis plants, meanwhile stetting up a control group (without the
Description
pedicel-inducing mixed solution sprayed thereon). 30 pots of plants pertain to each processing, repeatedly trialed 3 times. They are processed with the pedicel-inducing mixed solution once every W days to 3 times in total. The spraying method includes using a 150m1 small sprinkler each time, and adjusting its mouth to the best state of forming mists applied in one cycle around the stem segments of 2-5 leaves of the Phalaenopsis plants, with stress on spraying the leaf axils of the plants, after spraying, it is suitable that the water mist adheres to the surface of the stem segments and does not flow. The trial results show that the stem-doubling induction rate of the plants sprayed with the pedicel-inducing mixed solution is 98.9% for floweret, 88.9% for grandiflora, so the stem-doubling induction rate of floweret is 10% higher than that of grandiflora, and the average stem-doubling induction rate of floweret and grandiflora is 93.9%,but the stem-doubling induction rate of the plants without the pedicel-inducing mixed solution spayed thereon is quite low. The detailed trial results are shown in Table 4, wherein, P9-Pio involve the pedicel-inducing mixed solution A: TDZ(0.03g/L)+GA3(0.6g/L) +IAA(0.4g/L)+P 205(0.5 -1.2g/L)+glutamic acid( 1.2g/L); Pi-P12 involve the pedicel-inducing mixed solution B: TDZ(0.03g/L)+0A3(0.6g/L) +I4A(0.4g/L)+PP333(0.5g/L)+P205(0.9g/L); P13-P14 involve the pedicel-inducing mixed solution C: TDZ(0.03g/L)+0A3(0.6g/L) +IAA(0.4g/L)+P P333(0. 5 g/L)+P205(0 98/L) + glutami c acid (1. 2g/L); P15-P16 involve the pedicel-inducing mixed solution D: TDZ(0.03g/L)+0A3(0.6g/L) +1AA(0.4g/L) +P205(0.9g/L).
Table 4 Comparison on different Phalaenopsis plants with the pedicel-inducing mixed solution sprayed thereon Procc Solution conbination Number Number Inducti Pedicel Pcdicel ssing (mg -1,1) (plants) of double on rate length diamete NO. stems (%) (cm) r (cm) (plants) P9 Plants sprayed with the 90 72 80 10.9 0.41 pedicel-inducing mixed solution A (grandiflora) Pic, Plants sprayed with the 90 81 90 8.6 0.30 pedicel-inducing mixed solution A (floweret)
Description
Pil Plants sprayed with the 90 73 81.1 9.2 0.45 pedicel-inducing mixed solution B (grandiflora) Pr Plants sprayed with the 90 82 91.1 7.7 0.35 pedicel-inducing mixed solution B (floweret) P13 Plants sprayed with the 90 80 88.9 12.8 0.52 pedicel-inducing mixed solution C (grandiflora) P I 4 Plants sprayed with the 90 89 98.9 10.5 0.38 pedicel-inducing mixed solution C (floweret) P15 Plants sprayed with the 90 70 77.8 7.9 0.39 pedicel-inducing mixed solution D (grandiflora) P16 Plants sprayed with the 90 80 88.9 6.6 0.25 pedicel-inducing mixed solution D (floweret) P3 Control group (without the mixed solution sprayed thereon) (grandiflora) 90 0 0.0 0 0 P4 Control group (without the mixed solution sprayed thereon) (floweret) 90 1 1.1 5.5 0.23 According to the statistical analysis after 30 days, the result of spraying the pedicel-inducing mixed solution A on the Phalaenopsis plants after completing the step of uniformly adjusting the orientation and spatial angle shows that: the number of the inducted double stems of the Phalaenopsis grandiflora in the group P9 is 72, as 80% as a stem-doubling induction rate, the pedicel length is 10.9cm and the pedicel diameter is 0.41cm, the number of the inducted double stems of the Phalaenopsis floweret in the group Pio is 81, as 90% as a stem-doubling induction rate, the pedicel length is 8.6cm and the pedicel diameter is 0.31cm.
According to the statistical analysis after 30 days, the result of spraying the pedicel-inducing mixed solution B on the Phalaenopsis plant after completing the step of uniformly adjusting the orientation and spatial angle shows that: the number of the inducted double stems of the Phalaenopsis grandiflora in the group Pii is 73, as 81.1% as a stem-doubling induction rate, the pedicel length is 9.2cm and the pedicel diameter is 0.45cm; the number of the inducted double
Description
stems of the Phalaenopsis floweret in the group P12 is 82, as 91.1% as a stem-doubling induction rate, the pedicel length is 7.7cm and the pedicel diameter is 0.35cm.
According to the statistical analysis after 30 days, the result of spraying the pedicel-inducing mixed solution C on the Phalaenopsis plant after completing the step of uniformly adjusting the orientation and spatial angle shows that: the number of the inducted double stems of the Phalaenopsis floweret in the group P14 is 89, as 98.9% as a stem-doubling induction rate, the pedicel length is 10.5cm and the pedicel diameter is 0.38 cm (see FIGs.3, 6 and 7); the Phalaenopsis grandiflora in the group P13 comes second, the number of its inducted double stems is 80, as 88.9% as a stem-doubling induction rate, the pedicel length is 12.8cm and the pedicel diameter is 0.52cm (see FIGs.4, 5 and 8) . According to the statistical analysis after 30 days, the result of spraying the pedicel-inducing mixed solution D on the Phalaenopsis plant after completing the step of uniformly adjusting the orientation and spatial angle shows that: the number of the inducted double stems of the Phalaenopsis grandiflora in the group P15 is 70, as 77.8% as a stem-doubling induction rate, the pedicel length is 7.9cm and the pedicel diameter is 0.39cm; the number of the inducted double stems of the Phalaenopsis floweret in the group P16 is 80, as 88.9% as a stem-doubling induction rate, the pedicel length is 6.6cm and the pedicel diameter is 0.25cm.
It can be seen from Table 4 that: after completing the step of uniformly adjusting the orientation and spatial angle, the effect of spraying the pedicel-inducing mixed solutions (TDZ(0.01-0.05g/L)+GA3(0.3-1.0g/L)+IAA(0.1-0.6g/L)+PP333(0.2-0.8g/L)+P205 (0.5-1.2g/L)+gl utamic acid (0.8-1.5g/L)) on the Phalaenopsis plants is the best in the aspect of whether the number of the induced double stems or the improvement of the induction rate. Among them, the Phalaenopsis floweret in the group P14 has the best induction effect with the maximum number of 89 inducted double stems, and the induction rate up to 98.9%, the pedicel length is 10.5cm and the pedicel diameter is 0.38cm (see FIGs.3, 6 and 7). The Phalaenopsis grandiflora in the group P13 comes second, the number of its inducted double stems is 80, as 88.9% as a stem-doubling induction rate, the pedicel length is 12.8cm and the pedicel diameter is 0.52cm (see FIGs.4, 5 and 8). Compared with P3,P4,P9,Pio,PII,P12,P13,P15 and P16, the number of the inducted double stems of the Phalaenopsis floweret in the group P14 rises by 89, 88 as a rate of increase as 8800%, 17 as a rate of increase as 23.6%, 8 as a rate of increase as 9.9%, 16 as a rate of increase as 21.9%, 7 as a rate of increase as 8.5%, 9 as a rate of increase as 11.3%, 19 as a rate of increase as 24.1% and 9 as a rate
Description
of increase as 11.3%, respectively; its stem-doubling induction rate increases by 98.9%, 97.8%, 18.9%, 8.9%, 17.8%, 7.8%, 10.0%, 21.1% and 10.0%, respectively; its pedicel length rises by 10.5cm, 5cm as a rate of increase as 91%, -0.4cm as a rate of decrease as 3.7%, 1.9cm as a rate of increase as 22.1%, 1.3cm as a rate of increase as 14.1%, 2.8cm as a rate of increase as 36.4%, -2.3cm as a rate of decrease as 18.0%, 2.6cm as a rate of increase as 32.9% and 3.9cm as a rate of increase as 59.1%, respectively; its pedicel diameter rises by 0.38cm, 0.15cm as a rate of increase as 65.2%, -0.03cm as a rate of decrease as 7.3%, 0.08cm as a rate of increase as 26.7%, -0.07cm as a rate of decrease as 15.6%, 0.03cm as a rate of increase as 8.6%, -0.14cm as a rate of decrease as 26.9%, -0.01cm as a rate of decrease as 2.6% and 0.13cm as a rate of increase as 52%, respectively. It can be seen from the above that the slight partial disadvantage of the floweret in comparison with the grandiflora in the aspects of the pedicel length and pedicel diameter depends on the characteristics of the varieties themselves; but the floweret has an absolute advantage in the aspects of the key indexes, the number of inducted double stems and the stem-doubling induction rate. Compared with P3,P4,P9,Plo,P11,P12,P13,P15 and P16, the number of the inducted double stems of the Phalaenopsis grandiflora in the group P13 rises by 80, 79 as a rate of increase as 7900%, 8 as a rate of increase as 11.1%, -1 as a rate of decrease as 1.2%, 7 as a rate of increase as 9.6%, -2 as a rate of decrease as 2.4%, -9 as a rate of decrease as 10.1%, 10 as a rate of increase as 14.3% and 0 as a rate of increase as 0.0%, respectively; its stem-doubling induction rate increases by 88.9%, 87.8%, 8.9%, -L1%, 7.8%, -2.2%, -10.0%, 11.1% and 0.0%, respectively; its pedicel length rises by 12.8cm, 7.3cm as a rate of increase as 132.7%, 1.9cm as a rate of increase as 17.4%, 4.2cm as a rate of increase as 48.8%, 3.6cm as a rate of increase as 39.1%, 5.1cm as a rate of increase as 66.2%, 2.3cm as a rate of increase as 21.9%, 4.9cm as a rate of increase as 62.0% and 6.2cm as a rate of increase as 93.9%, respectively; its pedicel diameter rises by 0.52cm, 0.29cm as a rate of increase as 126.1%, 0.11cm as a rate of increase as 26.8%, 0.22cm as a rate of increase as 73.3%, 0.07cm as a rate of increase as 15.6%, 0.17cm as a rate of increase as 48.6%, 0.14cm as a rate of increase as 36.8%, 0.13cm as a rate of increase as 33.3% and 0.27cm as a rate of increase as 108.0%, respectively. It can be seen from the above that the grandiflora has an obvious disadvantage in comparison with the floweret in the aspects of the number of inducted double sterns and the stem-doubling induction rate, but has an advantage in the aspects of the pedicel length and the pedicel diameter.
In summary, the method of applying the pedicel-inducing mixed solutions after completing the
Description
step of uniformly adjusting the orientation and spatial angle plays a crucial role in the induction of double stems of Phalaenopsis, not only achieving a "zero" breakthrough (Phalaenopsis grandiflora), but also significantly increasing the number of the inducted double stems and raising the stem-doubling induction rate. In addition, the method also has an obvious advantage in increasing the pedicel length and pedicel diameter as a solid technical foundation in the aspects of cultivating high-yield, high-quality and efficient phalaenopsis and beautifying environments for people's yearning life.
The above description is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any modifications, equivalent replacements and improvements made by any person skilled in the art within the technical scope disclosed by the present invention shall fall within the protection scope of the present invention.

Claims (3)

  1. C 1 a i in s What is claimed is: I. A method for high-efficiently inducing, double stems of Phalaenopsis characterized in that said method comprises the following steps: S t. physical processing of plants: uniformly adjusting the physical plane and spatial angle of Phalaenopsis plants to be processed for flower forcing; and 52. processing with a chemical reagent. after completing Si, spraying a pedicel-inducing mixed solution on leaves and stem segments of said Phalaenopsis plants; ending with enabling double stems of Phalaenopsis to be high-efficiently induced, wherein in Si, the step of adjusting the physical plane includes arranging all Phalaenopsis plants toward an identical orientation with the growth direction of the stems and leaves of said Phalaenopsis plants toward north and their roots toward south; in S t, the step of adjusting the spatial angle includes raising the inclination of all the Phalaenopsis plants to be processed for flower forcing to an identical angle, so as to enable light to strike the leaf axils of the stem segments of 3-5 leaves of said Phalaenopsis plants from top to bottom, after growing to 10-20 cm for pedicels under flower forcing for 30 to 40 days, removing the inclination and returning to arrangements at 0 or 180 degrees; in S2, said pedicel-inducing mixed solution includes TDZ(0.01-0.05g/L)+GA3(0.3-1.0g/L)+ I A A(0. 1-0.6g/L)+PP333(0. 2-0.8g/L)+P205(0.5-1.2g/L)+ glutami c acid (O. 8-1.5g/L).
  2. 2. The method for high-efficiently inducing double stems of Phalaenopsis according to claim 1, characterized in that in S2, the spraying method includes spraying in one cycle around the stem segments of 2-5 leaves of said Phalaenopsis plants by using a small sprinkler each time, with stress on spraying the leaf axils of said Phalaenopsis plants, after spraying, it is suitable that the water mists adhere to the surface of the stem segments and does not flow.
  3. 3. The method for high-efficiently inducing double stems of Phalaenopsis according to claim 1, characterized in that in S2, said pedicel-inducing mixed solution is sprayed once every 9-12 days to 2-4 times in total.
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