CN109197544B - Dwarfing method of aquatic canna - Google Patents

Dwarfing method of aquatic canna Download PDF

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CN109197544B
CN109197544B CN201811275861.5A CN201811275861A CN109197544B CN 109197544 B CN109197544 B CN 109197544B CN 201811275861 A CN201811275861 A CN 201811275861A CN 109197544 B CN109197544 B CN 109197544B
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aqueous solution
canna
spraying
aquatic
soaking
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沈士华
李梅姿
裘金玉
曹小英
黄槿烽
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Yuhang District Wetland Ecological Research Institute
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants

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  • Biodiversity & Conservation Biology (AREA)
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Abstract

A dwarfing method of aquatic canna belongs to the technical field of agriculture. Which comprises the following steps: 1) selecting aquatic canna indica seeds to perform pot culture; 2) and when the aquatic canna potted plant grows to bud of 4-5 cm 2-3 cm, sequentially carrying out root soaking or spraying treatment by using paclobutrazol aqueous solution, mepiquat chloride aqueous solution and uniconazole aqueous solution, and cultivating to obtain the dwarfed aquatic canna. According to the invention, the canna aquatica is sequentially treated by the paclobutrazol aqueous solution, the mepiquat chloride aqueous solution and the uniconazole aqueous solution, so that the dwarfing effect can be realized; and the special nutrient solution is combined, the plant height can be obviously reduced, the plant is compact and short, the height of the treated plant is 1/2-1/3 shorter than that of a control plant, the color of the leaf is dark, the width of the leaf is increased by 20-40%, and the thickness is increased by 10-20%.

Description

Dwarfing method of aquatic canna
Technical Field
The invention belongs to the technical field of agriculture, and particularly relates to a dwarfing method of aquatic canna.
Background
The dwarfing technology of plants is very commonly applied to terrestrial plants, and particularly, the dwarfing technology of plants is used as a main variety for landscape cultivation, such as fruit tree bonsais, stump bonsais, flowers (such as cockscomb flowers) and the like. The rice, the staple food of the vast majority of people in China, is a very typical example of successful dwarfing. The rice straws of the rice in the fifth and sixty years of the last century are very long and high, almost more than fifty rice plants are laid down every year, the rice ears are as long as possible and are easy to fall off when the rice ears are heavier, and the rice straws are laid down and the rice seeds are mildewed and germinate in the ground, so that the yield and the quality of the rice seeds are seriously influenced. The height of the rice pole is almost 50-60% of the old rice pole, and almost no lodging occurs. Although not belonging to aquatic plants, rice grows in water for a lifetime very similar to that of aquatic plants.
At present, the dwarfing of terrestrial plants generally adopts the following methods: A. removing terminal buds, removing main branches and carrying out purposeful pruning; B. asexual propagation such as layering, cuttage, grafting and the like is used; C. manual intervention such as Qugan, girdling, button, needling, carving (such as daffodil), etc.; D. radiation treatment such as gamma ray treatment of narcissus bulb, cobalt 60 treatment of terrestrial canna; E. spraying, soaking root, mixing with soil, etc. with plant growth regulator, such as PP333, ethylene, chlormequat chloride, shapen, paclobutrazol, mepiquat chloride, B9; F. combines gene genetic technology and tissue culture technology.
Generally, terrestrial plants can adopt relatively more methods and are simpler to operate. The land plants grow on the ground, so that the operation is convenient, and the individual plant quantity is small; and the manual operation of the aquatic plants growing in water is very difficult. Therefore, the method A, B, C is not suitable for aquatic plants. Method F is the most advanced technique, but requires a high demand for a series of complete talent teams, laboratories and specialties. Usually done by institutional universities and research institutes. For example, the university of the Chinese sowthistle in seawater has developed the Chinese sowthistle strain with excellent performance in the whole seven years.
The dwarfing of the tape grass of the university of seawater is realized by combining the gene technology and the tissue culture technology. Is the only practical example of the successful dwarfing of aquatic plants.
To date, the aquatic plant dwarfing technology has not had a second successful example other than tape grass. Little research and attention has been directed to this work. As the application of aquatic plants is increased, the exposure problems are increased, such as lodging, breakage of plants on the floating island and even turning over or scattering of the whole floating island; the secondary pollution of large amount of plants, the overgrowth of submerged plants to the surface of water body seriously affects the water body landscape, the plants wither and die in winter every year, the too high pole is not beneficial to the production of pot seedlings and the like, so the call and the requirement for the energy improvement of the aquatic plants are stronger and stronger.
As for the potted seedling production of aquatic plants, China is in a very original state at present, and the aquatic plants are usually only moved from the ground to the culture pots for planting without any technical content and technical processing. With the improvement of living standard of people, the requirements on the quality, style and cleanliness of flowers are higher and higher, the original soil-culture flowers cannot meet the market demands, and the water-culture flowers are produced at the right moment. Hydroponic flowers are a model of scientific agriculture and high-efficiency agriculture in the world, and the development of hydroponic flowers is not well developed in some developed countries. The water culture flower production in China is in the beginning and rising period, and is one of the directions of flower development. At present, terrestrial plants have a plurality of varieties and types of water culture flowers. Has a certain market size and capacity. But the hydroponic flowers of aquatic plants are completely a blank. However, the water culture flower production of the aquatic plants also needs to adopt dwarfed varieties, otherwise, the products with beautiful shapes cannot be produced.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to design and provide a technical scheme of a dwarf method of aquatic canna.
The dwarf method of the aquatic canna is characterized by comprising the following steps:
1) selecting aquatic canna indica seeds to perform pot culture;
2) and when the aquatic canna potted plant grows to bud of 4-5 cm 2-3 cm, sequentially carrying out root soaking or spraying treatment by using paclobutrazol aqueous solution, mepiquat chloride aqueous solution and uniconazole aqueous solution, and cultivating to obtain the dwarfed aquatic canna.
The dwarf method of the aquatic canna is characterized in that the root soaking treatment in the step 2) is specifically as follows: soaking the roots in 80-180ppm paclobutrazol water solution for 4-6 hours, and drying in the shade; soaking the root in 20-80ppm mepiquat chloride solution for 4-6 hr, and drying in the shade; then the roots are soaked in 30-100ppm uniconazole aqueous solution for 4-6 hours and dried in the shade.
The dwarf method of the canna aquatica is characterized in that the spraying treatment in the step 2) is specifically as follows: when 4-5 buds of 2-3 cm grow out of the aquatic canna, spraying 80-180ppm of paclobutrazol aqueous solution for the first time; spraying 20-80ppm mepiquat chloride aqueous solution for the second time after 3 days from the first spraying; and 3 days after the second spraying, carrying out the third spraying by adopting 30-100ppm uniconazole aqueous solution.
The dwarfing method of the aquatic canna is characterized in that the potting medium adopts biogas slurry containing 0.5 percent of monopotassium phosphate.
The dwarf method of the canna aquatica is characterized in that the shade drying time is 4-6 hours.
The dwarfing method for the aquatic canna has reasonable design, and the dwarfing effect can be realized by sequentially treating the aquatic canna through the paclobutrazol aqueous solution, the mepiquat chloride aqueous solution and the uniconazole aqueous solution; and the special nutrient solution is combined, the plant height can be obviously reduced, the plant is compact and short, the height of the treated plant is 1/2-1/3 shorter than that of a control plant, the color of the leaf is dark, the width of the leaf is increased by 20-40%, and the thickness is increased by 10-20%.
Detailed Description
The present invention is further illustrated by the following examples.
Example 1: selection of dwarfing agent concentration
1) The concentration and preparation of the dwarfing agent are as follows: the prepared concentration is divided into a liquid storage and a liquid use, the liquid storage can be stored in the shade for three months, and the liquid use can be prepared and used immediately.
The concentrations and formulation methods of dwarfing agents are given in table 1:
Figure 415580DEST_PATH_IMAGE001
2) dwarfing treatment method
First, potted plant test
And the four series of paclobutrazol, mepiquat chloride, uniconazole and a reference meter are respectively sprayed and soaked in roots according to the concentration of the table, the total amount is 80 groups, and each group of ten pot seedlings is obtained.
Planting amount of seedlings in each pot: the amount of canna seedlings in each pot is 4 buds.
The requirements of seedling supply are as follows: the seedlings to be tested must be robust and disease-free, and the plant height and the thickness of stems and leaves among pots are consistent.
The requirements of the potting soil are as follows: the planting soil must be mixed evenly, and the soil consumption of each pot is equal.
Root soaking time: the control group was simultaneously soaked in water for 12 hours.
Spraying: spraying for the first time on the next day after planting; spraying for the second time 3 days after the first spraying; the third spraying is carried out 3 days after the second spraying.
② ground cultivation test
The concentration of the dwarfing agent used is the same as that of the potting test, and the root soaking method is also the same.
Preparing land cultivation land: selecting plots with the same conditions of illumination, soil quality and water quality, loosening and thinning soil on the surface of the land leveling layer, wherein the selected land requires a certain distance from other aquatic plants, and mutual interference is avoided.
Land area: approximately two square meters per test group were planted with 10 clusters per square meter with 4 shoots of canna plants per cluster. The plant selection criteria were the same as for the pot. The plants in each test group were substantially similar.
(iii) Observation and recording
Recording the content: dwarfing dose type and concentration, method of use, time, weather, air temperature, water temperature, growth conditions (plant height, leaf color, leaf thickness, etc.)
3) Effect of dwarfing plants
Table 2 stunting effect of paclobutrazol treated potted seedlings beginning 3 months and 5 days (with newly increased height of leaves)
Figure 674261DEST_PATH_IMAGE002
TABLE 3 stunt Effect of mepiquat chloride treated potted seedlings beginning 3 months and 5 days (with newly increased height of leaves)
Figure 878977DEST_PATH_IMAGE003
Table 4 stunt effect of uniconazole treated potted seedlings beginning 3 months and 5 days (with newly increased height of leaves)
Figure 15561DEST_PATH_IMAGE004
From the table, the dwarfing effect of paclobutrazol on iris is increased along with the lengthening of time, the effect is not obvious within 4-10 days, and the dwarfing effect is obviously increased after 20 days; meanwhile, the dwarfing effect is obviously increased along with the increase of the concentration, the inhibition effect is especially obvious when the concentration is 80-150PPM, and the effect is slowed down after the concentration of 150 PPM. Thus, the optimal concentration of paclobutrazol to dwarf canna is 80-180 PPM. In addition, the optimal concentration of uniconazole is 30-100 PPM; the optimum concentration of mepiquat chloride is 20-80 PPM.
Example 2: selection of combinations of dwarfing agents
Root soaking treatment by adopting single dwarfing agent and combined dwarfing agent
Test 1: soaking roots in 150ppm paclobutrazol water solution for 12 hours;
test 2: soaking the root for 12 hours by 80ppm of mepiquat chloride aqueous solution;
test 3: 60ppm of uniconazole aqueous solution is soaked in the root for 12 hours;
test 4: soaking roots in 150ppm paclobutrazol water solution for 4 hours, and drying in the shade for 4 hours; soaking the root in 80ppm mepiquat chloride solution for 4 hr, and drying in shade for 4 hr; then soaking the roots for 4 hours by using 60ppm uniconazole aqueous solution, and drying in the shade for 4 hours;
comparison: and (5) treating with clear water.
Table 5: stunning effect starting at 5 days of 3 months (with newly increased height of leaves)
Figure 684439DEST_PATH_IMAGE005
As can be seen from Table 5, the dwarfing effect can be significantly improved by using the combined dwarfing agent for root soaking treatment.
Example 3: selection of combinations of dwarfing agents
Spraying single dwarfing agent and combined dwarfing agent until the leaf surface is wet.
Test 1: spraying 150ppm paclobutrazol water solution;
test 2: spraying 80ppm mepiquat chloride aqueous solution;
test 3: spraying 60ppm uniconazole aqueous solution;
test 4: spraying 150ppm paclobutrazol water solution for the first time; spraying the mepiquat chloride aqueous solution of 80ppm for the second time after 3 days from the first spraying; spraying for the third time by adopting 60ppm uniconazole aqueous solution after spraying for 3 days from the second time;
comparison: and (5) treating with clear water.
Table 6: stunning effect starting at 5 days of 3 months (with newly increased height of leaves)
Figure 615486DEST_PATH_IMAGE006
As can be seen from Table 6, the dwarfing effect can be significantly improved by applying the combined dwarfing agent.
Example 4: selection of nutrient solutions
Comparison: water culture king sold in market;
test 1: the preparation method of the biogas slurry containing 0.5% of monopotassium phosphate comprises the following steps: taking biogas slurry obtained by fermenting pig farm sewage with biogas, filtering with 0.45u of medium speed paper, dehydrating, deodorizing, and adding potassium dihydrogen phosphate 0.5% of the weight of the biogas slurry.
After the potting test, compared with the canna of the control group, the leaf color of the aquatic canna of the test group 1 is dark and turns into dark green, the thickness of the leaf is thickened and the width is widened, the recovery period of the aquatic canna is shortened, and the root promoting time is shortened.
Example 5:
1) selecting aquatic canna seeds to carry out pot culture, wherein a pot culture substrate is biogas slurry containing 0.5% of monopotassium phosphate;
2) when 4 buds of 2 cm grow out of the aquatic canna potted plant, sequentially carrying out root soaking treatment by using a paclobutrazol aqueous solution, a mepiquat chloride aqueous solution and an uniconazole aqueous solution, soaking the roots for 4 hours by using a 160ppm paclobutrazol aqueous solution, and drying in the shade; soaking the root in 80ppm mepiquat chloride solution for 4 hr, and drying in shade; then soaking the roots in 60ppm uniconazole aqueous solution for 4 hours, drying in the shade, and cultivating to obtain dwarf aquatic canna.
The height of the treated plant is 1/2% shorter than that of the control plant, the color of the leaf is darker, the width of the leaf is increased by 30%, and the thickness is increased by 15%.
Example 6:
1) selecting aquatic canna seeds to carry out pot culture, wherein a pot culture substrate is biogas slurry containing 0.5% of monopotassium phosphate;
2) when 4 buds of 2 cm grow out of the aquatic canna potted plant, sequentially spraying paclobutrazol aqueous solution, mepiquat chloride aqueous solution and uniconazole aqueous solution, and firstly spraying 80ppm paclobutrazol aqueous solution for the first time; spraying the mepiquat chloride aqueous solution with the concentration of 40ppm for the second time after 3 days from the first spraying; and after 3 days from the second spraying, spraying 30ppm of uniconazole aqueous solution for the third time, and cultivating to obtain dwarf aquatic canna.
The height of the treated plant is 1/3% shorter than that of the control plant, the leaf color is darker, the width of the leaf is increased by 25%, and the thickness is increased by 10%.

Claims (3)

1. A dwarf method of aquatic canna is characterized by comprising the following steps:
1) selecting aquatic canna indica seeds to perform pot culture;
2) when the aquatic canna potted plant grows to bud of 4-5 and 2-3 cm, sequentially adopting paclobutrazol aqueous solution, mepiquat chloride aqueous solution and uniconazole aqueous solution to carry out root soaking or spraying treatment, and cultivating to obtain dwarfed aquatic canna; the root soaking treatment specifically comprises the following steps: soaking the roots in 80-180ppm paclobutrazol water solution for 4-6 hours, and drying in the shade; soaking the root in 20-80ppm mepiquat chloride solution for 4-6 hr, and drying in the shade; then soaking the roots for 4-6 hours by using 30-100ppm uniconazole aqueous solution, and drying in the shade, wherein the spraying treatment specifically comprises the following steps: when 4-5 buds of 2-3 cm grow out of the aquatic canna, spraying 80-180ppm of paclobutrazol aqueous solution for the first time; spraying 20-80ppm mepiquat chloride aqueous solution for the second time after 3 days from the first spraying; and 3 days after the second spraying, carrying out the third spraying by adopting 30-100ppm uniconazole aqueous solution.
2. The dwarf method of canna aquatica according to claim 1, wherein the potting medium is biogas slurry containing 0.5% monopotassium phosphate.
3. The method for dwarfing canna aquatica according to claim 1, characterized in that the drying time in the shade is 4-6 hours.
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CN110972857A (en) * 2019-12-02 2020-04-10 甘肃步云农牧科技有限责任公司 Seedling raising method for improving emergence rate of radix bupleuri
CN113575393A (en) * 2021-05-26 2021-11-02 上海清涟环境科技有限公司 Domestication method of submerged plants

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