CN114560736B - Composite nutrition regulator for regulating bougainvillea spectabilis florescence and regulating method thereof - Google Patents
Composite nutrition regulator for regulating bougainvillea spectabilis florescence and regulating method thereof Download PDFInfo
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Abstract
The invention provides a compound nutrition regulator for regulating and controlling bougainvillea spectabilis florescence and a regulating and controlling method thereof. The application discovers for the first time that under the stress of melatonin and drought, the differentiation time of bougainvillea spectabilis buds can be shortened, the flowering time of the bougainvillea spectabilis is can be obviously advanced, growth hormones such as ethephon, monopotassium phosphate and the like for promoting the differentiation of the bougainvillea spectabilis can be matched, the time required for flowering can be further synergistically shortened, meanwhile, sucrose can promote the synthesis of bougainvillea spectabilis pigment, the composite nutrition regulator is obtained after the components are compounded, and the composite nutrition regulator is used in the regulation and control of the flowering phase of the bougainvillea spectabilis, can obviously shorten the flowering time, prolong the flowering phase time and enhance the color of the bougainvillea spectabilis. The composite nutrient regulator for regulating and controlling the bougainvillea spectabilis florescence and the regulating and controlling method thereof can promote the bougainvillea spectabilis to bloom, shorten the time for blooming, prolong the whole florescence open time and greatly improve the appreciation of the bougainvillea spectabilis.
Description
Technical Field
The invention provides a compound nutrition regulator for regulating and controlling bougainvillea spectabilis florescence and a regulating and controlling method thereof, belonging to the technical field of agriculture.
Background
Bougainvillea spectabilis willld, leaf flower of the same name, mirabilis jalapa and the like are evergreen climbing shrubs with high ornamental value in the genus of Nyctaginaceae (Bougainavillea), and flowers are the main ornamental parts. Bougainvillea spectabilis, originally found in brazil, has now been introduced worldwide, including southern provinces such as fujian, taiwan, and guangdong in China, and is widely used in urban landscaping. With the increasing importance of urban environment beautification, the economic value and the ornamental value of bougainvillea spectabilis are in urgent need of development.
When the bougainvillea spectabilis is applied to landscaping, the problems of vain growth of branches and leaves, disordered flowering phase, scattered plant types and poor ornamental value are frequently caused, the decoration and ornamental effect is seriously influenced, and the problem needs to be solved urgently. Therefore, foreign scholars have long studied the flowering mechanism, flower bud differentiation mechanism and flowering phase regulation technology of bougainvillea spectabilis. The university of California published an article in 1964 detailing its research efforts on the flowering mechanism of bougainvillea spectabilis. Hackett et al studied the morphological dissection of the inflorescence protomer of bougainvillea spectabilis and studied the systematic study of the flowering process and inflorescence structure of bougainvillea spectabilis by Lopez in order to investigate the morphological changes during the differentiation period of bougainvillea spectabilis. Early research results of foreign scholars preliminarily reveal the flowering process of bougainvillea spectabilis.
The bougainvillea spectabilis flowering phase regulating and controlling technology mainly belongs to two main categories, in the aspect of physical technology, saifuddin and other researches show that the bougainvillea spectabilis is are increased in pruning times to promote more flower buds, even-Chen and other researches show that the photosynthesis rate of mature leaves of bougainvillea spectabilis is improved when the bougainvillea spectabilis grows in a short-day environment, and Chenghuaqing researches show that the bract service lives of two varieties are obviously prolonged after shade domestication treatment is carried out on two varieties of 'purple flower' and 'Taibeihong'. Chemical technologyIn the aspect of operation, tse and the like adopt two treatment methods of removing new leaves and cytokinin to research the influence on the process of differentiating the trigonal plum blossom sequences. Selection of exogenous GA by Steffen et al 3 The effect on inflorescence differentiation was investigated. The use of Saifuddin et al and Moneruzzaman et al is 100mg -1 NAA, GA 3 The mixture is used for treating bougainvillea spectabilis, and meanwhile, the new leaves of the bougainvillea spectabilis are removed, so that the bract service life can be remarkably prolonged, and the bract quality can be improved.
At present, researches on regulating and controlling the flowering phase of bougainvillea spectabilis mainly focused on the directions of variety breeding, flower bud differentiation, flower bud life, growth and development and the like. Researches are carried out on how to improve bougainvillea spectabilis flowering phase time, anthocyanidin content and the like, and a special nutrition regulator for regulating and controlling the bougainvillea spectabilis flowering phase is lacked, so that the technical problems of uneven flowering phase, uneven flower color and inconsistent quality standard in industrial production of bougainvillea spectabilis are urgently to be solved.
Disclosure of Invention
The invention aims to provide a composite nutrient regulator for regulating and controlling bougainvillea spectabilis florescence and a regulating and controlling method thereof.
The application provides a compound nutrition regulator for regulating and controlling bougainvillea spectabilis florescence, which consists of the following components: every 1L of purified water contains 100-200 mu mol of melatonin, 20-30mg of ethephon, 1-2 g of cane sugar and 2-4 g of monopotassium phosphate.
Further, the compound nutrition regulator consists of the following components: each 1L of purified water contains 100 mu mol of melatonin, 20mg of ethephon, 2g of sucrose and 2g of monopotassium phosphate.
The application also provides a bougainvillea spectabilis florescence regulation and control method, which comprises the following steps:
(1) Selecting annual potted bougainvillea spectabilis, and culturing at 24-26 ℃ and 70-80% humidity indoors;
(2) The potting soil is a mixed matrix of nutrient soil, and the volume ratio is 1;
(3) The concentration of melatonin is selected to be 100 mu mol/L, and distilled water is used for preparing a solution;
(4) Carrying out 3-day seedling revival treatment on bougainvillea spectabilis, specifically watering in the morning and watering thoroughly, spraying 100 mu mol/L melatonin on the leaf surface of the bougainvillea spectabilis, and stopping watering after 3 days to enable the bougainvillea spectabilis to naturally dry for 14-21 days;
(5) After drought treatment, if wet watering is seen, spraying the compound nutrient regulator of claim 1 on the leaf surface every 2 days for 1 time at night until 1 month after flowering;
(6) Fertilization was noted during flowering, and topdressing was performed 1 time per week.
And (5) further, stopping watering after 3 days in the step (4) to enable the plants to naturally dry for 14 days.
Further, bougainvillea spectabilis flowering period control treatment is performed around the first 50d of the expected flowering period of bougainvillea spectabilis.
Furthermore, the growth in the bougainvillea spectabilis period needs to be in a full-light and ventilated habitat state.
Further, the nutrient soil in the step (2) is prepared from coconut coir: peat: the volume ratio of the perlite is 1.
Further, the base fertilizer of the pot soil in the step (2) is prepared by adding 15-9-11 g of Ordovician Green slow release fertilizer into each L of mixed matrix.
Further, in the flowering period in the step (6), nitrogen, phosphorus and potassium 20-20-20 water-soluble fertilizer is additionally applied for 1 time every 7 days, and the dilution concentration is 1000 times.
Has the beneficial effects that:
1. the application provides a composite nutrient regulator for regulating and controlling bougainvillea spectabilis florescence and a regulating and controlling method thereof, which can promote bougainvillea spectabilis to bloom, shorten the time for blooming, prolong the whole florescence open time and greatly improve the ornamental value of the bougainvillea spectabilis.
2. Years of experiments show that the water control treatment is a key factor for prolonging the florescence of bougainvillea spectabilis, the bougainvillea spectabilis moderate in demand for soil water content, and the florescence of the bougainvillea spectabilis difficult to control due to excessive water. The study finds that the melatonin can relieve the influence of plant drought stress, the mild drought stress and the melatonin treatment can obviously advance the flowering time of bougainvillea spectabilis through a large number of comparative tests, the gradient change presented by the soil water content of the bougainvillea spectabilis very obvious along with the extension of the drought time, the experiment finds that the ground diameter increment of the bougainvillea spectabilis treated by natural drought treatment (ZG) presents a descending trend along with the extension of the stress treatment time of the bougainvillea spectabilis, and the ground diameter increment of the bougainvillea spectabilis treated by the foliar melatonin spraying Treatment (TG) presents a descending trend after increasing. At the time of water cut 14d, the ZG-treated land size began to shrink, while the TG-treated land size was still increasing, at which point the increase in land size reached a maximum. Similarly, the seedling height also shows the same trend, and the melatonin treated bougainvillea seedling height increment is the highest when the water is stopped for 14 d.
3. The composite nutrient regulator is prepared by compounding the components, and can obviously shorten the flowering time, prolong the flowering time and brighten the color of bougainvillea spectabilis.
4. The effect that the blooming is advanced by 37 days in the initial flowering period compared with the control group cultured by normal watering can be achieved through the joint regulation of drought stress and the compound nutrition regulator, and the blooming time from the full flowering period to the final flowering period is prolonged by 25 days compared with the control group. By different compound nutrient regulator compound formulas, a better test group is obtained, namely the drought stress of 14d is treated by adding melatonin than that of 21d, the flowering time can be obviously shortened, and the flowering period is prolonged.
5. In the control group which is only subjected to drought stress, or only subjected to drought stress plus melatonin treatment, or only sprayed with a plurality of compound nutritional regulators, the flowering time of the control group is delayed compared with that of the control group which uses the test example, and the flowering phase is shortened. For the control example using drought stress + melatonin treatment in the early stage, the control treatment using one component of the complex nutrient regulator alone all showed a delay in flowering time and a reduction in flowering time compared to the combination using the test examples of the present application. The effects of advancing flowering and delaying the flowering phase can be realized by jointly adjusting the drought stress and the composite nutrition regulator.
6. The composite nutrition regulator can regulate the flowering phase of bougainvillea spectabilis, can improve the content of anthocyanidin, is obtained by compounding different composite nutrition regulators according to different compounds, is singly compounded by one or more of melatonin, ethephon, cane sugar or potassium dihydrogen phosphate, and is sprayed on bougainvillea spectabilis leaves, the anthocyanidin content is not used, the composite nutrition regulator prepared by compounding 100 mu mol/L MT +20mg/L E +2g/L S +2g/L P is good in effect, the flower color of the bougainvillea spectabilis enhanced by matching with drought stress treatment, and particularly when the full-bloom stage is open, the treatment is beneficial to the accumulation of the anthocyanidin in the bougainvillea spectaculata buds, the flower color is more bright, and the ornamental value is increased.
7. The matrix proportion and the fertilization measure can further promote flower bud differentiation in the bougainvillea spectabilis florescence, and the nutrient soil proportion is coconut coir: peat: the volume ratio of perlite is 1. In different comparison examples of the Ordovician greens slow release fertilizer, the influence difference of the Ordovician greens slow release fertilizer on the flowering number is not obvious, and the influence difference of different treatments of substrate proportion and additional fertilizer on the flowering number of bougainvillea spectabilis is very obvious.
Drawings
FIG. 1 graph of changes in soil water content under natural drought stress
FIG. 2 is a graph showing the change of bougainvillea spectabilis ground diameter increment under drought stress and melatonin treatment
FIG. 3 change diagram of bougainvillea spectabilis seedling high increment under drought stress and melatonin treatment
Detailed Description
The processing method of the present invention will be described below with reference to specific examples, but the present invention is not limited thereto. The reagents and materials and equipment are commercially available, unless otherwise specified.
Example 1
A composite nutrition regulator for regulating bougainvillea spectabilis florescence comprises the following components: every 1L of purified water contains 100 mu mol of melatonin, 20mg of ethephon, 2g of sucrose and 2g of monopotassium phosphate.
Example 2
A composite nutrition regulator for regulating bougainvillea spectabilis florescence comprises the following components: every 1L of purified water contains 100 mu mol of melatonin, 30mg of ethephon, 2g of sucrose and 2g of monopotassium phosphate.
Example 3
A method for regulating bougainvillea spectabilis florescence, wherein the regulation treatment can be carried out at about the first 50d of the expected flowering period of bougainvillea spectabilis, and the method comprises the following steps:
(1) Selecting annual potted bougainvillea spectabilis, and culturing at the indoor temperature of 25 ℃ and the indoor humidity of 70%;
(2) The potting soil is nutrient soil, coconut husk: peat: the volume ratio of the perlite is 1; the base fertilizer of the pot soil is prepared by adding 15-9-11 g of Ordovician Green slow release fertilizer into each L of mixed matrix;
(3) The concentration of melatonin is selected to be 100 mu mol/L, and distilled water is used for preparing a solution;
(4) Carrying out 3-day seedling revival treatment on bougainvillea spectabilis, specifically watering in the morning and watering thoroughly, spraying 100 mu mol/L melatonin on the leaf surface of the bougainvillea spectabilis, and stopping watering after 3 days to enable the bougainvillea spectabilis to naturally dry for 14 days;
(5) After drought treatment, if the plants are watered in a dry and wet manner, the compound nutrition regulator in the embodiment 1 or 2 is sprayed on the leaf surfaces of the plants every 2 days until 1 month after flowering; the bougainvillea spectabilis is growing in a full-light and ventilated habitat state;
(6) Topdressing nitrogen phosphorus potassium 20-20-20 water soluble fertilizer for 1 time every 7 days in the flowering period, diluting the fertilizer by 1000 times, and completely watering.
Test one: effect of drought stress and melatonin on morphological growth of bougainvillea spectabilis
The test method comprises the following steps:
annual potted bougainvillea spectabilis (hereinafter referred to as bougainvillea spectabilis) seedlings are used as test materials, all the seedlings grow strongly and are consistent in growth vigor, and no plant diseases or insect pests exist. The pot soil is a mixed matrix of nutrient soil, the bottom diameter of the flowerpot is 30cm, the height of the flowerpot is 40.5cm, and each seedling is potted. Before the beginning of the experimental treatment, the bougainvillea spectabilis seedling is moved into a greenhouse, the indoor temperature is 24-26 ℃, and the humidity is 70-80%.
The test is divided into two treatments, namely natural drought treatment (ZG) and natural drought + foliage spray melatonin Treatment (TG), each treatment is repeated for 3 times, each treatment is repeated for 10 pots, and the prophase pretest shows that when 100 mu mol/L melatonin is sprayed on the leaves of the bougainvillea spectabilis seedlings, the growth state of the seedlings is the best, so that the concentration of the melatonin selected in the test is 100 mu mol/L, and the solution is prepared by using distilled water.
Before the test is started, 3 days of seedling revival are carried out on the bougainvillea spectabilis, two treated bougainvillea spectabilis are watered thoroughly at 9 points in the morning every day, and watering is stopped after 3 days to enable the bougainvillea spectabilis to be naturally dry; meanwhile, in the seedling recovering period, 100 mu mol/L melatonin is sprayed on the surfaces of the TG-treated bougainvillea spectabilis leaves at 17 pm every day for 3 times, and the spraying amount is based on that the leaves are full of water drops and do not flow down in a strand manner. Judging the drought degree and the severe drought according to the relative water content of the soil: 15% -25%, moderate drought: 25% -35%, mild drought: 35 to 45 percent. Sampling is carried out once every 7d during drought, and sampling is respectively carried out at 0, 7, 14, 21, 28 and 35 days for measuring relevant indexes. Sampling on day 0 as a control group (CK), selecting bougainvillea spectabilis plants with excellent growth, observing the phenotypic change of the bougainvillea spectabilis plants, measuring the water content of soil, the plant height and the ground diameter of the bougainvillea spectabilis plants, and measuring again when sampling for the last time. The experiment was conducted in a modern intelligent greenhouse (120.63 ° E,28.00 ° N) in wenzhou city, zhejiang, 2021, month 10.
And (3) test results:
as shown in figure 1 (note: letters a, b, etc. indicate that the difference between the data is significant at the level of 0.05 according to the LSD test), after the bougainvillea spectabilis subjected to drought stress treatment, the soil water content of the bougainvillea spectabilis shows a continuous reduction trend, the water content in the CK period is 80.10%, the water content is reduced significantly in the 0d-14d after the drought treatment, and the reduction trend of the water content is gradually gentle in the later period of stress. In the test, when water is cut off for 21 days, the mild drought stress level is reached, and the water content of the soil at the moment is 35.27%; when the water is stopped for 28 days, the moderate drought stress level is reached, and the water content is 27.65 percent; the severe drought stress level is reached when the water is cut off for 35 days, and the water content is 19.30 percent. Along with the prolonging of drought time, the gradient change presented by the water content of the bougainvillea spectabilis soil is very obvious, and the process of gradually losing the soil water in the natural environment is effectively reflected.
As shown in FIG. 2 (note: letters a, b, etc. indicate significance of difference at the level of 0.05 between the data according to LSD test), the increment of bougainvillea glabra diameter by natural drought treatment (ZG) showed a decreasing trend as the treatment time for bougainvillea glabra was prolonged; at 28d (moderate) and 35d (severe) of water cut, the increase in the ground diameter showed a negative increase, indicating that drought stress inhibited the growth of bougainvillea speetalis and caused a certain amount of change in the external morphology of bougainvillea.
As shown in fig. 2, the increase in the ground diameter of bougainvillea spectabilis treated by foliar melatonin Treatment (TG) showed a tendency of increasing first and then decreasing as the stress treatment time for bougainvillea spectabilis prolonged. At the time of water cut 14d, the ZG-treated land size began to shrink, while the TG-treated land size was still increasing, at which point the increase in land size reached a maximum.
As shown in FIG. 3 (note: letters a, b, etc. indicate significance of difference at the level of 0.05 between the data according to LSD test), the ZG-treated bougainvillea seedling height increment shows a trend of ascending first and then descending as the time for stress treatment on bougainvillea glabra is prolonged; at water cut 7d to 21d (mild), the seedling height increase showed a rising trend. The water is stopped for 21d (mild) to 35d (severe), and the seedling height increment shows a descending trend.
As shown in fig. 3, the TG-treated bougainvillea seedling high gain also showed a tendency to rise first and then fall as the stress treatment time for bougainvillea was extended. Compared with each period in the test, the seedling height increment of TG treatment is higher than that of ZG treatment, and is respectively higher by 3.9 percent, 9.2 percent, 7.1 percent, 5.1 percent and 0.5 percent. It shows that the melatonin treated bougainvillea was the highest in the increase of the height of bougainvillea spectabilis at 14d after water supply was stopped.
And (2) test II: effect of drought stress and Complex Nutrition regulators on bouquet stage control
The test method comprises the following steps: the first test is continued, the test is carried out at 1 month and 10 days in 2021, drought stress and melatonin treatment are carried out, normal watering is used as a control (2 times of watering every week till thorough watering), the treatment of the compound nutrition regulator is carried out at 14 days and 21 days respectively, specifically, the compound nutrition regulator as defined in claim 1 is sprayed on the leaf surface every 2 days until 1 month after flowering, the treatment mode is shown in table 1, wherein 3 times of treatment are carried out, 10 pots of treatment are carried out for each time, and the flowering time is observed. Recording the florescence: counting the date of initial flowering on the days that more than half of the plants are flowering; the full-bloom period counted by the day that more than 50% of the treated plants have flowers open; the final florescence date is counted by the day when more than 50% of flowers of each treated plant start wilting, and the flowering duration is counted by the number of days between the full florescence and the final florescence.
And (3) test results: the effect of flowering 37 days earlier than the initial flowering phase of the control group 1 can be achieved through the combined regulation of drought stress and the compound nutrition regulator, and the full-bloom period of the test example 1 is also 54 days longer than that of the control group 1, and the flowering time from the full-bloom period to the final flowering period is also 25 days longer than that of the control group 1 (57 days). In experimental examples 2-4, by different compound nutrition regulator compound formulas, a better experimental group is obtained, namely the treatment of drought stress 14d plus melatonin can obviously shorten the time for flowering and prolong the flowering period. When the drought stress time is prolonged to 21d, the flowering time of bougainvillea spectabilis is also delayed because the growth and development of bougainvillea spectabilis are obviously influenced, and the flowering time is obviously shortened compared with the treatment of the drought stress 14 d.
In the control group subjected to only drought stress, or treated with only drought stress plus melatonin, or treated with only spraying of several kinds of composite nutrient regulators (control examples 2 to 4), it was found that the flowering time was delayed and the flowering period was shortened as compared with the flowering time of the combination using the test examples. For the control cases using drought stress + melatonin treatment in the early stage, the control treatments using one component of the complex nutritional regulator alone (see control cases 5 to 8) all showed a delay in flowering-time and a reduction in flowering-time compared to the combination using the test cases of the present application. The effects of advancing flowering and delaying the flowering phase can be realized by jointly adjusting the drought stress and the composite nutrition regulator.
TABLE 1 Effect of drought stress and Complex Nutrition regulators on bouquet control
Wherein: melatonin-MT, ethephon-E, sucrose-S, potassium dihydrogen phosphate-P
And (3) test III: influence of drought stress and composite nutrient regulator on bougainvillea spectabilis pigment content
The test method comprises the following steps:
the test was continued according to the method of test two, and the anthocyanidin was measured by taking the flower bud pieces at different periods, and the flower buds of each test group were randomly taken, and each treatment was repeated 3 times. The extraction and content determination method of the plant anthocyanidin is as follows:
the method comprises the following steps: weighing 1g of finely ground bougainvillea spectabilis bract, and soaking with 30ml of 5% ethanol at room temperature.
Step two: leaching with 5% ethanol 30ml at room temperature for three times, and filtering after 24 h.
Step three: concentrating the crude pigment extract, removing pectin with 75% ethanol, and vacuum evaporating to obtain concentrated anthocyanidin solution.
Step four: preparing a pigment solution to be detected by mixing the pigment concentrated solution with 1.5mol/L HCl-95% ethanol according to the ratio of 15 to 85, measuring an absorbance value in a 550nm range by using an ultraviolet-visible spectrophotometer, recording data, and analyzing to obtain an absorption spectrogram and a maximum absorption wavelength.
And (3) test results:
according to the comparison examples 5-10, the nutrition regulator obtained by compounding different composite nutrition regulators is obtained by singly using one or more of melatonin, ethephon, sucrose or monopotassium phosphate, the nutrition regulator obtained by compounding the melatonin, ethephon, sucrose or monopotassium phosphate is sprayed on the leaves of bougainvillea spectabilis, the effect of the formula of the application test example 1 is not good, the nutrition regulator of 100 mu mol/L MT +20mg/L E +2g/L S +2g/L P is adopted to be matched with the treatment of drought stress, the flower color of the bougainvillea spectabilis can be brightened, the accumulation of the anthocyanidin in the full-bloom stage is obviously promoted, and when the full-bloom stage is open, the treatment is beneficial to the accumulation of the anthocyanidin in the bougainvillea spectabilis, the flower color is brighter, and the ornamental value is increased.
TABLE 2 Effect of Complex Nutrition regulators on bougainvillea spectabilis pigment content
Wherein: melatonin-MT, ethephon-E, sucrose-S, potassium dihydrogen phosphate-P
And (4) testing: influence of substrate proportion and fertilization measures on flower bud differentiation in bougainvillea spectabilis period
The test method comprises the following steps: different substrate proportions, fertilization modes and gradients are set, the processing modes are shown in table 3, wherein each processing mode is 3 times of repetition, each processing mode is 10 pots of repetition, and the flowering number is calculated on the 10 th day of the full-bloom period.
And (3) test results: the matrix formula and the fertilization measure of the test examples 1 and 2 are matched with the tests from one to three, and the flowering period regulation and control treatment of the composite nutrient additive can further promote the flower bud differentiation in the full-bloom period of the bougainvillea spectabilis, thereby increasing the flowering number. The test of different substrate ratios shows that the coconut husk: peat: 1, perlite 1, add 15-9-11 of AoLu slow release fertilizer in the matrix, blooming effect is the best when nitrogen phosphorus potassium water soluble fertilizer 20-20-20, bougainvillea spectabilis comparatively drought-resistant afraid of wet waterlogging, through many years' study, discover that coconut husk and perlite cooperate and use, can promote bougainvillea spectabilis root portion drainage, prevent soil ponding, the coconut husk can improve higher lignocellulose for bougainvillea spectabilis growth, and its higher soil permeability, water retention capacity, can promote bougainvillea spectabilis root portion to nutrient absorption, prevent wet waterlogging simultaneously.
15-9-11 of Ordovician greens is used as a slow release fertilizer base fertilizer, and the nitrogen-phosphorus-potassium water soluble fertilizer is applied for 1 time per week in the flowering period, so that the differentiation of bouquets in the flowering period can be obviously promoted, the differentiation of the bouquets in the new branches is promoted differently by comparing the non-additional fertilizer in the growth period and the difference of the additional fertilizer frequency or the additional fertilizer amount, and the additional fertilizer is applied in the flowering period by matching with the flowering period regulation composite nutrition regulator disclosed by the application, so that the number of flowers in the flowering period can be increased, and the ornamental value can be improved.
In different comparison examples of the Ordovician greens slow release fertilizer, the influence difference of the Ordovician greens slow release fertilizer on the flowering number is not obvious, and the influence difference of different treatments of substrate proportion and additional fertilizer on the flowering number of bougainvillea spectabilis is very obvious.
TABLE 3 influence of substrate ratio and fertilization measures on flower bud differentiation in bougainvillea spectabilis
Although the present invention has been described with reference to a preferred embodiment, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (7)
1. A compound nutrition regulator for regulating and controlling bouquet rose florescence is characterized by comprising the following components: every 1L of purified water contains 100-200 mu mol of melatonin, 20-30mg of ethephon, 1-2g of cane sugar and 2-4g of monopotassium phosphate;
the flowering phase regulation is carried out at about the first 50d of the expected flowering phase of bougainvillea spectabilis, and the use method of the compound nutrient regulator in the flowering phase regulation of the bougainvillea spectabilis is as follows:
(1) Selecting annual potted bougainvillea spectabilis, and culturing at 24-26 ℃ and 70-80% humidity indoors;
(2) The substrate is a mixed substrate of potting soil and nutrient soil, and the volume ratio is 1;
(3) The concentration of melatonin is selected to be 100 mu mol/L, and distilled water is used for preparing a solution;
(4) Carrying out 3-day seedling revival treatment on bougainvillea spectabilis, specifically watering in the morning and watering thoroughly, spraying 100 mu mol/L melatonin on the leaf surface of the bougainvillea spectabilis, and stopping watering after 3 days to enable the bougainvillea spectabilis to naturally dry for 14-21 days;
(5) After drought treatment, if the plants are watered in a dry and wet manner, the compound nutrition regulator is sprayed on the leaf surfaces for 1 time every 2 days until 1 month after flowering;
(6) Fertilization is carried out during flowering, and topdressing is carried out for 1 time per week.
2. The compound nutritional regulator according to claim 1, wherein the compound nutritional regulator consists of: every 1L of purified water contains 100 mu mol of melatonin, 20mg of ethephon, 2g of sucrose and 2g of monopotassium phosphate.
3. The composite nutrient regulator of claim 1, wherein the watering is stopped after 3 days of step (4) to allow natural drought for 14 days.
4. The composite nutrient regulator of claim 1, wherein bougainvillea spectabilis florescence is grown under full light and ventilated habitat conditions.
5. The compound nutrition regulator of claim 1, wherein the nutrient soil of step (2) is coconut coir: peat: the volume ratio of perlite is 1.
6. The compound nutritional regulator of claim 1, wherein in step (2), 6g of azure slow-release fertilizer 15-9-11 is added per L of the mixed matrix.
7. The compound nutrition regulator as defined in claim 1, wherein the water soluble fertilizer of NPK 20-20-20 is additionally applied every 7 days in the flowering period in step (6) at a dilution concentration of 1000 times.
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