NL2028355B1 - Container seedling substrate for lithocarpus polystachyus and seedling raising method - Google Patents

Container seedling substrate for lithocarpus polystachyus and seedling raising method Download PDF

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NL2028355B1
NL2028355B1 NL2028355A NL2028355A NL2028355B1 NL 2028355 B1 NL2028355 B1 NL 2028355B1 NL 2028355 A NL2028355 A NL 2028355A NL 2028355 A NL2028355 A NL 2028355A NL 2028355 B1 NL2028355 B1 NL 2028355B1
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seedling
container
substrate
present disclosure
biomass
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NL2028355A
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NL2028355A (en
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He Ping
Li Yueqiao
Yu Longhua
Zhang Huacong
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Experimental Center Of Subtropical Forestry Chinese Acad Of Forestry
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A01G24/23Wood, e.g. wood chips or sawdust
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A01G24/25Dry fruit hulls or husks, e.g. chaff or coir
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/28Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/02Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
    • A01G9/029Receptacles for seedlings

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Botany (AREA)
  • Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The present disclosure provides a container seedling substrate for Lilhocarpus polyslachyus, and relates to the technical field of plant growing. The substrate includes peat, rice husk-sawdust mixture and yellow subsoil; the peat, the rice husk-sawdust mixture, and the yellow subsoil have a volume ratio of (3-5):(6-2):(l-3). The present disclosure further provides a container seedling raising method for L. polyslachyus. According to the method of the present disclosure, a cutting seedling of L. polyslachyus is transplanted into a non-woven seedling raising bag supplemented with seedling substrate and slow-release fertilizer for cultivation. By adjusting the container seedling substrate ratio for L. polyslachyus, the application amount of the slow-release fertilizer and the specification of a cultivation container, the present disclosure increases growth indexes including the seedling height, ground diameter, height-diameter ratio, aboveground biomass, root biomass, stem biomass, leaf biomass, and total biomass of a container seedling of L. polyslachyus, providing a scientific basis for cultivating high-quality container seedlings of L. polyslachyus.

Description

CONTAINER SEEDLING SUBSTRATE FOR LITHOCARPUS POLYSTACHYUS AND SEEDLING RAISING METHOD TECHNICAL FIELD
[01] The present disclosure relates to the technical field of plant growing, and in particular to a container seedling substrate for Lithocarpus polystachyus and a seedling raising method.
BACKGROUND ART
[02] Lithocarpus polystachyus is a tree species of the genus Lithocarpus of the family Fagaceae, mainly distributed in the provinces south of the Yangtze River in China. Traditionally, the folks often harvest L. polystachyus leaves for making tea, which has a long drinking history. Modern medicine has confirmed that Z. polystachyus is rich in a variety of nutrients, trace elements and vitamins necessary for the human body; phloridzin, trilobatin and flavonoids are the main biological active ingredients thereof, which have anti-inflammatory, antioxidant, hypoglycemic/hypolipidemic, and diabetes-controlling effects. Because Z. polystachyus is mostly available from wild resources, natural habitats thereof have been destroyed and wild resources have declined sharply with the increase in resource usage. Therefore, it is very necessary to carry out the artificial breeding and cultivation of L. polystachyus to conserve the natural germplasm of 1. polystachyus and meet the demand for high-quality raw materials in the future.
[03] At present, the research on the artificial cultivation of L. polystachyus has just started, mainly focusing on the research on cutting seedling raising technology of L. polvstachvus, and effects of planting density and light on seedling survival and sapling growth. Compared with ordinary bare-root seedlings, container seedlings of forest trees have the advantages of well-developed root system, easy transport, long afforestation season, high survival rate, and appropriateness for afforestation at difficult sites. Corresponding container seedling experiments have been carried out in roburs and oaks, like Quercus aliena, Quercus variabilis, Quercus ilex, and Quercus robur, and have achieved excellent results. For example, container seedlings of Quercus velutina cultivated by RPM technology can reach 2.5 times the diameter at breast height (DBH) of ordinary bare-root seedlings after 10 years of afforestation. L. polystachyus has the typical characteristics of tree species of the family Fagaceae. That is, the seedlings have developed axial roots, sparse lateral roots, and low afforestation survival rate, and grow slowly. Cultivating large-sized container seedlings is conducive to improving the morphological quality and the level of nutrient pools in the tissue, increasing the afforestation survival rate and promoting stand growth. However, there are relatively few studies on the cultivation of large-sized container seedlings of Z.
polystachyus. Therefore, in order to improve the quality of the seedlings of 1. polystachyus, it is very necessary to carry out research on the raising technology of container seedlings of I.
- 2.
polvstachvus.
SUMMARY
[04] In view of this, an objective of the present disclosure is to provide a container seedling substrate for L. polystachyus and a seedling raising method; the present disclosure can increase growth indexes including the seedling height, ground diameter, height-diameter ratio, aboveground biomass, root biomass, stem biomass, leaf biomass, and total biomass of a container seedling of /.
polvstachvus, providing a scientific basis for cultivating high-quality container seedlings of Z.
polystachyus.
[05] To solve the above technical problems, the present disclosure provides the following technical solutions:
[06] a container seedling substrate for Z. polystachyus is provided, including peat, rice husk-sawdust mixture, and yellow subsoil; the peat, the rice husk-sawdust mixture and the yellow subsoil have a volume ratio of (3-5):(6-2):(1-3).
[07] Preferably, the rice husk-sawdust mixture may include decomposed rice husk and decomposed sawdust; the decomposed rice husk and the decomposed sawdust may have a volume ratio of (1-1.5):1.
[08] The present disclosure further provides a container seedling raising method for Z. polystachyus, including the following steps:
[09] transplanting a cutting seedling of I. polystachyus into a seedling container for cultivation; where the seedling container contains the above container seedling substrate for L. polystachyus and a slow-release fertilizer.
[10] Preferably, the cutting seedling of Z. polystachyus may be a 1-year-old cutting seedling.
[11] Preferably, the seedling substrate and the slow-release fertilizer may be mixed, filtered and packed into the seedling container.
[12] More preferably, the filtration may be screen filtration, and the screen may have a pore size of 0.8-1.5 cm.
[13] Preferably, the seedling container may be a seedling raising bag, and the specification of the seedling raising bag may be 10 x 15 cm to 15 * 20 cm in terms of diameter and length.
[14] Preferably, in the seedling container, the slow-release fertilizer and the seedling substrate may have a mass-volume ratio of (2.0-4.0) kg: (0.9-1.1) m’.
[15] Preferably, the slow-release fertilizer may be a long-acting controlled-release fertilizer.
[16] More preferably, the slow-release fertilizer may include available elements nitrogen, phosphorus, and potassium; the nitrogen, the phosphorus, and the potassium may have a mass ratio of (2-4):(1-2):(1-3).
[17] The present disclosure has the following beneficial effects:
-3-
[18] The present disclosure provides a container seedling substrate for ZL. polvstachyus and a seedling raising method. By adjusting the container seedling substrate ratio for L. polystachyus, the application amount of the slow-release fertilizer and the specification of a cultivation container, the present disclosure may increase growth indexes including the seedling height, ground diameter, height-diameter ratio, aboveground biomass, root biomass, stem biomass, leaf biomass, and total biomass of a container seedling of L. polystachvus, and improve the seedling quality of L. polystachyus, providing a scientific basis for cultivating high-quality container seedlings of I. polvstachvus.
DETAILED DESCRIPTION OF THE EMBODIMENTS
[19] The present disclosure provides a container seedling substrate for L. polystachyus, including peat, rice husk-sawdust mixture, and yellow subsoil; the peat, the rice husk-sawdust mixture and the yellow subsoil have a volume ratio of (3-5):(6-2):(1-3).
[20] In the present disclosure, the peat, the rice husk-sawdust mixture, and the yellow subsoil may have a volume ratio of (3-5):(6-2):(1-3), and preferably 3:6:1.
[21] Inthe present disclosure, the peat may be selected reasonably according to different regions; the source of the peat is not particularly limited in the present disclosure, and all peat from known sources may be used in the present disclosure. In the specific examples of the present disclosure, peat deposits in northeast China may be preferred.
[22] In the present disclosure, the rice husk-sawdust mixture may preferably include decomposed rice husk and decomposed sawdust. In the present disclosure, the decomposed rice husk and the decomposed sawdust may preferably have a volume ratio of (1-1.5):1. In the specific examples of the present disclosure, the rice husk and the sawdust may be selected reasonably according to different regions and the difficulty of acquisition. In the present disclosure, the rice husk and sawdust native to Jiangxi may be preferred.
[23] The present disclosure further provides a container seedling raising method for I. polvstachyus, including the following steps: transplanting a cutting seedling of L. polystachvus into a seedling container for cultivation; where the seedling container contains the above container seedling substrate for /.. polystachyus and a slow-release fertilizer.
[24] Inthe present disclosure, the cutting seedling of L. polystachyus may be a 1-year-old cutting seedling. The present disclosure does not have special requirements on the source and growth status of the cutting seedlings of LZ. polystachyus, and it is preferable that there is no disease, insect damage or mechanical damage. In the specific examples of the present disclosure, the cutting seedlings may be preferably 1-year-old cutting seedlings with consistent growth from Jiangxi.
[25] In the present disclosure, the seedling substrate and the slow-release fertilizer may be and after mixed, filtered and packed into the seedling container. In the present disclosure, the filtration
- 4 - may preferably be screen filtration; the screen may preferably have a pore size of 0.8-1.5 cm, and more preferably 1 cm. The mixing and screening methods are not particularly limited in the present disclosure, as long as conventional mixing and screening methods in the art may be used.
[26] In the present disclosure, the seedling container may preferably be a seedling raising bag; the specification of the seedling raising bag may preferably be 10 x 15 cm to 15 * 20 cm in terms of diameter x length. The source and material of the seedling raising bag is not particularly limited in the present disclosure. In the specific examples of the present disclosure, the seedling raising bag may be preferably a non-woven seedling raising bag collectively produced in Anhui.
[27] In the present disclosure, in the seedling container, the slow-release fertilizer and the seedling substrate may preferably have a mass-volume ratio of (2.0-4.0) kg: (0.9-1.1) m®, and more preferably 3.0 kg: 1 m®. In the present disclosure, the slow-release fertilizer may preferably be a long-acting controlled-release fertilizer. In the present disclosure, the slow-release fertilizer may preferably include available elements nitrogen, phosphorus, and potassium; the nitrogen, the phosphorus, and the potassium may preferably have a mass ratio of (2-4):(1-2):(1-3), and more preferably 3:1:2. The source of the slow-release fertilizer is not particularly limited in the present disclosure, and all existing known sources of slow-release fertilizers may be used in the present disclosure. In the specific examples of the present disclosure, the slow-release fertilizer may preferably be APEX long-acting controlled-release fertilizer.
[28] In order to make the objectives, technical solutions and advantages of the present disclosure clearer, the present disclosure will be described in detail below in conjunction with examples, but they should not be construed as limiting the protection scope of the present disclosure.
[29] Examplel
[30] Cutting seedlings of L. polystachyus were cultivated in seedling containers with a diameter x length of 15 x 20 cm. Seedling substrates in the seedling containers were separately set as follows: Al: peat, rice husk-sawdust mixture and yellow subsoil mixed in a ratio of 3:6:1; A2: peat, rice husk-sawdust mixture and yellow subsoil mixed in a ratio of 4:4:2; and A3: peat, rice husk-sawdust mixture and yellow subsoil mixed in a ratio of 5:2:3; 3.0 kg of slow-release fertilizer was added per 1 m® of the substrate, and growth indexes of container seedlings of L. polystachvus were measured to obtain Table 1 below.
[31] Table 1 Effects of different substrate ratios on the growth indexes of container seedlings of L. polystachvus ID Substrate Seedling Ground Height-diameter Root Stem Leaf Total Root-shoot ratio height/em diameter/mm ratio biomass, biomass. biomass. biomass, ratio g/seedling giseedling gofseedling g/seedling Al 3601 22.538 4.768 46.575 1.588 1374 2.436 5.398 2.482 A2 442 22.267 4.932 44.798 1.736 1.438 2.770 5.943 2.499 A3 5:23 35.933 5.757 62.217 2.042 2.270 3.535 7.847 2.955
[32] From Table 1, different substrate ratios have different effects on the growth indexes of the
- 5. container seedlings of L. polystachyus. Particularly, when A3 (peat, rice husk-sawdust mixture and yellow subsoil mixed in a ratio of 5:2:3) is used as the seedling substrate, all growth indexes of the container seedlings of ZL. polvstachvus are higher.
[33] Example 2
[34] Cutting seedlings of L. polystachyus were cultivated in seedling containers with the following diameter x length: CI: 5 # 10 cm; C2: 10 x 15 cm; and C3: 15 * 20 cm. The seedling substrate in the seedling containers was peat, rice husk-sawdust mixture and yellow subsoil mixed in a ratio of 5:2:3; 2.0 kg of slow-release fertilizer was added per 1 m’ of the substrate, and growth indexes of container seedlings of ZL. polystachyvus were measured to obtain Table 2 below.
[35] Table 2 Effects of different specifications of seedling raising bags on the growth indexes of container seedlings of ZL. polystachyus ID Specification Seedling Ground Height-diam Root biomass. Stem Leaf Total Root-sho of seedling height/c diameter cter ratio g/scedling biomass, biomass, biomass, ot ratio raising bag m /mm g/seedling g/scedling piseedling Cl Sem~l0cm 20367 4573 45.087 0.881 0.981 1.843 3.706 3.527 C2 Wemxlsem 25011 4.863 51.600 1.087 1317 2.302 4.706 3571 C3 1Semx20cm 2584 5468 46.833 1.991 2.038 3.229 7.258 3.016
[36] From Table 2, different specifications of seedling raising bags have different effects on the growth indexes of the container seedlings of Z. polvstachvus. Particularly, when a seedling raising bag with a diameter = length of 15 x 20 cm is used as the seedling container, the growth indexes of the container seedlings of L. polystachyus are higher.
[37] Example 3
[38] Cutting seedlings of L. polvstachvus were cultivated in seedling containers with a diameter x length of 15 * 20 cm. The seedling substrate in the seedling containers was peat, rice husk-sawdust mixture and yellow subsoil mixed in a ratio of 5:2:3; 2.0 kg (B1), 3.0 kg (B2), and 4.0 kg (B3) of slow-release fertilizers were added per 1 m® of the substrate, respectively, and growth indexes of container seedlings of ZL. polvstachvus were measured to obtain Table 3 below.
[39] Table 3 Effects of different application amounts of the slow-release fertilizer on the growth indexes of container seedlings of Z. polvstachvus ID Application Seedling Ground Heighi-diamete Root Stem Leaf Total Root-shoo amounts of height/c diameter/ rratio biomass, biomass, biomass, biomass. t ratio slow-release m mm g'seedlin g/seedlin g/seedlin g/seedlin fertilizer, kg/m? g 2 g g substrate BI 2.0 20367 4.573 45.087 0.881 0.981 1.843 3.706 3.527 B2 30 17.344 4.241 41.360 0.801 0.824 1.618 3.243 3.454 B3 4.0 20.178 4481 45.263 0.916 1014 1.872 3.802 3.282
[40] From Table 3, different application amounts of the slow-release fertilizer have different effects on the growth indexes of the container seedlings of I. polystachyus. Particularly, when the application amount of the slow-release fertilizer is 4.0 kg/m’ substrate, the growth indexes of the container seedlings of ZL. polvstachvus are higher.
-6 -
[41] Example 4
[42] The seedling raising base of the Experimental Center of Subtropical Forestry, Chinese Academy of Forestry in Fenyi County, Jiangxi Province was selected as the experimental site, and a steel frame greenhouse was arranged as a shadehouse of the seedling raising base. The shadehouse was 2.4 m in height, equipped with automatic sprinkler irrigation facilities; the transmittancy of the upper shading net was 70%.
[43] In late March, 2018, a substrate with peat, rice husk-sawdust mixture and yellow subsoil mixed in a volume ratio of 3:6:1 was mechanically stirred well with a slow-release fertilizer (4.0 kg/m’ substrate), and filtered through a 1 cm screen into non-woven seedling raising bags with a diameter = length of 15 = 20 cm. One-year-old cutting seedlings of L. polystachyus were transplanted into the seedling raising bags and placed on the seedling ground of the nursery shadehouse; water was sprayed in time to keep the substrate moist, and trays for the seedling substrate were loosened frequently to prevent roots from penetrating the container wall into the soil.
[44] Example 5
[45] The seedling raising base of the Experimental Center of Subtropical Forestry, Chinese Academy of Forestry in Fenyi County, Jiangxi Province was selected as the experimental site, and a steel frame greenhouse was arranged as a shadehouse of the seedling raising base. The shadehouse was 2.4 m in height, equipped with automatic sprinkler irrigation facilities; the transmittancy of the upper shading net was 70%.
[46] In late March, 2018, a substrate with peat, rice husk-sawdust mixture and yellow subsoil mixed in a volume ratio of 5:2:3 was mechanically stirred well with a slow-release fertilizer (4.0 kg/m’ substrate), and filtered through a 1 cm screen into non-woven seedling raising bags with a diameter x length of 15 x 20 cm. One-year-old cutting seedlings of L. polystachyus were transplanted into the seedling raising bags and placed on the seedling ground of the nursery shadehouse; water was sprayed in time to keep the substrate moist, and trays for the seedling substrate were loosened frequently to prevent roots from penetrating the container wall into the soil.
[47] Example 6
[48] The seedling raising base of the Experimental Center of Subtropical Forestry, Chinese Academy of Forestry in Fenyi County, Jiangxi Province was selected as the experimental site, and a steel frame greenhouse was arranged as a shadehouse of the seedling raising base. The shadehouse was 2.4 m in height, equipped with automatic sprinkler irrigation facilities; the transmittancy of the upper shading net was 70%.
[49] In late March, 2018, a substrate with peat, rice husk-sawdust mixture and yellow subsoil mixed in a volume ratio of 4:4:2 was mechanically stirred well with a slow-release fertilizer (3.0 kg/m’ substrate), and filtered through a 1 cm screen into non-woven seedling raising bags with a diameter * length of 10 * 15 cm. One-year-old cutting seedlings of L. polystachyus were
-7 - transplanted into the seedling raising bags and placed on the seedling ground of the nursery shadehouse; water was sprayed in time to keep the substrate moist, and trays for the seedling substrate were loosened frequently to prevent roots from penetrating the container wall into the soil.
[50] Example 7 |51] The seedling raising base of the Experimental Center of Subtropical Forestry, Chinese Academy of Forestry in Fenyi County, Jiangxi Province was selected as the experimental site, and a steel frame greenhouse was arranged as a shadehouse of the seedling raising base. The shadehouse was 2.4 m in height, equipped with automatic sprinkler irrigation facilities; the transmittancy of the upper shading net was 70%.
[52] In late March, 2018, a substrate with peat, rice husk-sawdust mixture and yellow subsoil mixed in a volume ratio of 4:4:2 was mechanically stirred well with a slow-release fertilizer (2.0 kg/m’ substrate), and filtered through a 1 cm screen into non-woven seedling raising bags with a diameter x length of 15 x 20 cm. One-year-old cutting seedlings of L. polvstachyus were transplanted into the seedling raising bags and placed on the seedling ground of the nursery shadehouse; water was sprayed in time to keep the substrate moist, and trays for the seedling substrate were loosened frequently to prevent roots from penetrating the container wall into the soil.
[53] Comparative Example 1
[54] The seedling raising base of the Experimental Center of Subtropical Forestry, Chinese Academy of Forestry in Fenyi County, Jiangxi Province was selected as the experimental site, and a steel frame greenhouse was arranged as a shadehouse of the seedling raising base. The shadehouse was 2.4 m in height, equipped with automatic sprinkler irrigation facilities; the transmittancy of the upper shading net was 70%.
[55] In late March, 2018, a substrate with peat, rice husk-sawdust mixture and yellow subsoil mixed in a volume ratio of 2:8:0 was mechanically stirred well with a slow-release fertilizer (2.0 kg/m’ substrate), and filtered through a 1 cm screen into non-woven seedling raising bags with a diameter ~ length of 5 * 10 cm. One-year-old cutting seedlings of L. polystachyus were transplanted into the seedling raising bags and placed on the seedling ground of the nursery shadehouse; water was sprayed in time to keep the substrate moist, and trays for the seedling substrate were loosened frequently to prevent roots from penetrating the container wall into the soil.
[56] Comparative Example 2
[57] The seedling raising base of the Experimental Center of Subtropical Forestry, Chinese Academy of Forestry in Fenyi County, Jiangxi Province was selected as the experimental site, and a steel frame greenhouse was arranged as a shadehouse of the seedling raising base. The shadehouse was 2.4 m in height, equipped with automatic sprinkler irrigation facilities; the transmittancy of the upper shading net was 70%.
[58] In late March, 2018, a substrate with peat, rice husk-sawdust mixture and yellow subsoil
- 8 - mixed in a volume ratio of 2:8:0 was mechanically stirred well with a slow-release fertilizer (1.5 kg/m’ substrate), and filtered through a 1 cm screen into non-woven seedling raising bags with a diameter * length of 10 » 15 cm. One-year-old cutting seedlings of L. polystachyus were transplanted into the seedling raising bags and placed on the seedling ground of the nursery shadehouse; water was sprayed in time to keep the substrate moist, and trays for the seedling substrate were loosened frequently to prevent roots from penetrating the container wall into the soil.
[59] Comparative Example 3
[60] The seedling raising base of the Experimental Center of Subtropical Forestry, Chinese Academy of Forestry in Fenyi County, Jiangxi Province was selected as the experimental site, and a steel frame greenhouse was arranged as a shadehouse of the seedling raising base. The shadehouse was 2.4 m in height, equipped with automatic sprinkler irrigation facilities; the transmittancy of the upper shading net was 70%.
[61] In late March, 2018, a substrate with peat, rice husk-sawdust mixture and yellow subsoil mixed in a volume ratio of 3:6:1 was mechanically stirred well with a slow-release fertilizer (1.5 kg/m) substrate), and filtered through a 1 cm screen into non-woven seedling raising bags with a diameter x length of 5 = 10 cm. One-year-old cutting seedlings of L. polystachyus were transplanted into the seedling raising bags and placed on the seedling ground of the nursery shadehouse; water was sprayed in time to keep the substrate moist, and trays for the seedling substrate were loosened frequently to prevent roots from penetrating the container wall into the soil.
[62] Example 8
[63] In December, 2018, after the I. polvstachyus seedlings in Examples 4 to 7 and Comparative Examples 1 to 3 stopped growing, the seedling height and ground diameter of each seedling in each example were measured, and the height-diameter ratio was calculated; the ground diameter was measured with a digital vernier caliper; the seedling height was measured with a tape measure, and the calculation formula of the height-diameter ratio was: height-diameter ratio = seedling height/ground diameter.
[64] 10 healthy plants were randomly selected from Examples 4 to 7 and Comparative Examples 1 to 3, respectively, and the biomass of the seedlings was measured. During biomass measurement, the seedlings of each example were divided into three parts: roots, stems, and leaves. After water-removing at 105°C for 2 h, the seedlings were dried in a constant temperature oven at 80°C to constant weight. The biomass of each part was measured with an electronic balance; root-shoot ratio was calculated, and the calculation formula of the root-shoot ratio was: root-shoot ratio = aboveground biomass/belowground biomass. Furthermore, the root length, root surface area, root diameter, root volume and number of root tips of the seedlings were measured with a WinRHIZO root-measuring system, and Tables 4 and 5 were obtained.
[65] Table 4 Growth indexes of L. polystachyus seedlings in different examples
-9- Ne R: en Lez Total Seedling Ground Height-diameter . oot Stem . caf . ota Root-shoot Group heiohtom diamelor/mm ratio biomass. biomass. biomass, biomass, ratio & ‘ © g/seedling g/seedling g/seedling g/seedling ‘ Example 4 34.833 6.023 59.927 2.887 3.073 4.550 10.510 3.169 Example 5 34.383 5.542 63.638 2.592 2.802 4.462 9.855 3.465 Example 6 36.000 5.527 67.123 2.682 2.518 4.392 9.592 2.845 Example 7 31.983 5.373 60.006 2.400 1.990 3.433 7.823 2.660 Comparative 53956 4.051 35.050 0.462 0.528 0.896 1.886 3.124 Example 1 Comparative») 10 4.729 47.439 1.272 1.227 2.029 4.528 2.583 Example 2 Companie 7867 4014 44.509 0.821 0.789 1.409 3.019 3.021 Example 3 —
[66] Table 5 Root indexes of Z. polystachyus seedlings in different examples Root-sl ‚ ‚ Number of root Group oo shoot Length/cm Surface area/cm? Diameter/mm Volume/cmy’ umber o root ratio tips/root tip Example 4 3.169 299.185 20.247 1.880 211.963 108.500 Example 3 3.465 335.236 22.439 1.853 137.759 57.833 Example 6 2.845 290.321 19.942 2.018 145.885 69.167 Example 7 2.660 313.554 21.280 1.962 146.789 85.500 Comparative 3.124 204.190 15.490 1.799 60.847 30.000 Example 1 Comparative 2.583 250.483 17.804 2.001 87.636 47.333 Example 2 Comparative 3.021 192.816 14.858 1.884 69.496 42.889 Example 3
[67] From Tables 4 and 5 above, the seedling raising methods for L. polystachyus in Examples 4 to 7 of the present disclosure can significantly improve the growth performance of L. polystachyus seedlings, and also help improve the root indexes of the L. polystachyus seedlings, providing a scientific basis for the cultivation of high-quality container seedlings of L. polystachyus.
[68] Example 9
[69] The membership function method was used to comprehensively evaluate the seedling raising indexes of different seedling raising methods of the foregoing Examples 4 to 7 and Comparative Examples 1 to 3, and Table 6 was obtained.
[70] To achieve data processing and statistical analysis, WPS Excel was used for basic data statistics, and SPSS 22.0 statistical software was used for membership function. The calculation formula of the membership function 1s: U(x;) = (Xi - Xmin)/(Xmax - Xmin), Where x; is a measured value of an index, Xmax is a maximum value of a processing index, and Xmin is a minimum value of a processing index.
[71] Table 6 The membership function evaluation of the growth and nutritional indexes of the container seedlings of L. polystachyus in different examples \ Average value of membership function Average value of membership Average value of integrated Group . . . . . of growth index function of root index membership function Example 4 0.8964 0.646 0.7712 Example 5 0.8046 0.5498 0.6772 Example 6 0.7767 0.5305 0.6336
- 10 - Example 7 0.6444 0.5876 0.616 vale v 0.1414 0.063 0.1022 FA 0.3684 0.3104 0.3304 Companiie 0.2057 0.109 0.1573
[72] From Table 6, the maximum of integrated average membership function value of Example 4 is 0.7712, and the average membership function values of growth and root indexes are 0.8964 and
0.646, respectively, ranking first, and showing the best seedling raising effect. Meanwhile, the above results indicate that, when raising L. polystachyus seedlings, if the optimal seedling substrate ratio, application amount of slow-release fertilizer, and specification of seedling raising bag are selected as seedling raising conditions, respectively, the final seedling raising effect of the container seedlings of Z. polystachyus may not be necessarily the best; the seedling substrate ratio, the application amount of slow-release fertilizer and the specification of the seedling raising bag influence each other, and jointly determine the seedling raising effect of ZL. polystachyus.
[73] It can be seen from the above-mentioned examples that the container seedling raising method for LZ. polystachyus provided by the present disclosure can significantly improve the growth indexes of L. polystachyus seedlings; the growth indexes, including seedling height, ground diameter, height-diameter ratio, aboveground biomass, root biomass, stem biomass, leaf biomass, and total biomass, are increased by 0.49-5.25 times. Meanwhile, the method of the present disclosure is beneficial to improve the root indexes of the L. polystachyus seedlings, in which the volume and the number of root tips are increased by 2.48 and 2.62 times, respectively, providing a scientific basis for the cultivation of high-quality container seedlings of L. polystachyus.
[74] The above are only the examples of the present disclosure and therefore do not limit the patent scope of the present disclosure. Any equivalent structure or equivalent process transformation used according to the contents of the specification in the present disclosure, no matter whether it is directly or indirectly used in any other related technical field, should be included within the scope of patent protection of the present disclosure.

Claims (10)

-11 - CONCLUSIES-11 - CONCLUSIONS 1. Een containerzaailingssubstraat voor Lithocarpus polystachyus, bestaande uit turf, rijstschil-zaagselmengsel en gele ondergrond; waarbij de turf, het rijstschil-zaagselmengsel en de gele ondergrond een volumeverhouding hebben van (3-5) : (8-2) : (1-3).1. A container seedling substrate for Lithocarpus polystachyus, consisting of peat, rice husk sawdust mixture and yellow substrate; wherein the peat, the rice husk-sawdust mixture and the yellow substrate have a volume ratio of (3-5) : (8-2) : (1-3). 2. Het containerzaailingssubstraat voor Lithocarpus polystachyus volgens conclusie 1, waarbij het rijstschil-zaagselmengsel ontbonden rijstschil en ontbonden zaagsel omvat; en de ontbonden rijstschil en het ontbonden zaagsel een volumeverhouding hebben van (1-1,5) : 1.The container seedling substrate for Lithocarpus polystachyus according to claim 1, wherein the rice husk-sawdust mixture comprises decomposed rice husk and decomposed sawdust; and the decomposed rice husk and decomposed sawdust have a volume ratio of (1-1.5) : 1. 3. Een werkwijze voor het kweken van zaailingen in een container voor Lithocarpus polystachyus, die de volgende stappen omvat: het verplanten van een stekzaailing van Lithocarpus polystachyus in een zaailingcontainer voor de teelt; waarbij de zaailingcontainer het zaailingsubstraat volgens conclusie 1 en een meststof met langzame afgifte bevat.A method of growing seedlings in a container for Lithocarpus polystachyus, comprising the steps of: transplanting a cutting seedling of Lithocarpus polystachyus into a seedling container for cultivation; wherein the seedling container contains the seedling substrate of claim 1 and a slow release fertilizer. 4. De werkwijze voor het kweken van zaailingen volgens conclusie 3, waarbij de stekzaailing van Lithocarpus polystachyus een 1-jarige stekzaailing is.The method of raising seedlings according to claim 3, wherein the cutting seedling of Lithocarpus polystachyus is a 1-year cutting seedling. 5. De werkwijze voor het kweken van zaailingen volgens conclusie 3, waarbij het zaailingsubstraat en de meststof met langzame afgifte worden gemengd, gefilterd en verpakt in de zaailingcontainer.The method of raising seedlings according to claim 3, wherein the seedling substrate and the slow release fertilizer are mixed, filtered and packed in the seedling container. 6. De werkwijze voor het kweken van zaailingen volgens conclusie 5, waarbij de filtratie zeeffiltratie is en de zeef een poriegrootte van 0,8 - 1,5 cm heeft.The method of raising seedlings according to claim 5, wherein the filtration is screen filtration and the screen has a pore size of 0.8 - 1.5 cm. 7. De werkwijze voor het kweken van zaailingen volgens conclusie 3 of 5, waarbij de zaailingcontainer een zak voor het kweken van zaailingen is en de specificatie van de zak voor het kweken van zaailingen 10 x 15 cm tot 15 x 20 cm is in termen van diameter x lengte.The method of raising seedlings according to claim 3 or 5, wherein the seedling container is a seedling raising bag and the specification of the seedling raising bag is 10 x 15 cm to 15 x 20 cm in terms of diameter x length. 8. De werkwijze voor het kweken van zaailingen volgens conclusie 3, waarbij in de zaailingcontainer de meststof met langzame afgifte en het zaailingsubstraat een massa- volumeverhouding hebben van (2,0-4,0) kg : (0,9-1,1) m3.The method of raising seedlings according to claim 3, wherein in the seedling container, the slow release fertilizer and the seedling substrate have a mass to volume ratio of (2.0-4.0) kg : (0.9-1.1 ) m3. 9. De werkwijze voor het kweken van zaailingen volgens conclusie 8, waarbij de meststof met langzame afgifte een langwerkende meststof met gecontroleerde afgifte is.The method of raising seedlings according to claim 8, wherein the slow release fertilizer is a controlled release sustained release fertilizer. -12--12- 10. De werkwijze voor het kweken van zaailingen volgens conclusie 9, waarbij de meststof met langzame afgifte de beschikbare elementen stikstof, fosfor en kalium omvat; en de stikstof, de fosfor en het kalium een massaverhouding hebben van (2-4) : (1-2) : (1-3).The method of raising seedlings according to claim 9, wherein the slow release fertilizer comprises the available elements nitrogen, phosphorus and potassium; and the nitrogen, phosphorus and potassium have a mass ratio of (2-4) : (1-2) : (1-3).
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