CN110024524A - A kind of efficient Maize at Seedling Stage salt endurance appraisement method - Google Patents
A kind of efficient Maize at Seedling Stage salt endurance appraisement method Download PDFInfo
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- CN110024524A CN110024524A CN201910311524.5A CN201910311524A CN110024524A CN 110024524 A CN110024524 A CN 110024524A CN 201910311524 A CN201910311524 A CN 201910311524A CN 110024524 A CN110024524 A CN 110024524A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
- A01C1/025—Testing seeds for determining their viability or germination capacity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The present invention relates to a kind of efficient Maize at Seedling Stage salt endurance appraisement methods, and steps are as follows: (1) using when determining corn seedling Salt-Tolerance Identification and carry out under constant 8 ‰ NaCl mass concentration；(2) vitality index, plant height, root long, plant fresh weight, overground part dry weight, underground part dry weight, leaf chlorophyll and the proline content, blade and root K+/Na+ ratio of the corn seed under constant 8 ‰ NaCl mass concentration processing are measured；(3) determine dry weight change rate (%) index, and determine the evaluation and test parameter between dry weight change rate (%) index and the salt tolerance of corn germplasm, evaluate Maize at Seedling Stage salt tolerance.This method uses constant salinity, realizes the salt tolerant performance that quick, precise Identification goes out corn, establishes the identification of Maize Seedling salt tolerance under constant salinity, assessment technique system, corn is efficiently produced and corn variety breeding has great importance.
Description
Technical field
The invention belongs to modern agricultural technology, especially a kind of efficient Maize at Seedling Stage salt endurance appraisement method.
Background technique
Corn is a kind of important grass family cereal crops, industrial crops and forage crop, cultivated area and total output
First, China Dou Ju.However salt tolerance of corn is weaker, maize under NaCl stress seedling growth is obstructed, seedling percent is low, produce
Amount decline, and China's solonchak distribution area is larger, this will restrict corn yield and quality.Rationally to utilize solonchak, corn is improved
Yield and quality.
Salt stress affects the sprouting and growth of vegetable seeds.Seed germination rate, germination percentage and germination index, which can be used as, to be commented
The index of valence seed vitality height；Salt gypsum rock can be used as the index of evaluation germination salt damage degree；Root/shoot ratio is evaluation seedling
The important indicator of phase root system development；Plant weights, root dry weight and moisture content are evaluation crops seedling stages by the effective of salt damage degree
Index.These indexs can reflect influence of the salt stress to plant germination and growth and development.High-salt stress will cause the hair of corn
Bud rate reduces, this is because the extraneous excessively high seed that results in of solution osmotic pressure cannot absorb moisture.Salt-tolerant plant has relatively high
Root/shoot ratio ensure that seedlings root physically well develops, while have stronger water absorbing capacity and absorb minerals ability, to make to plant
Object has saline-alkaline tolerance.High-salt stress inhibits the growth of aboveground vegetation part, the reason is that salinity inhibits and injure root, influences
Its ability for absorbing moisture and nutrient, and then decline plant water content.Germination percentage can reflect seed germination how much, root
Long, height of seedling can reflect the growing way of seedling, K+/Na+Ratio and offspring relative water content reflect that root of the seedling under salt stress is inhaled
Outlet capacity, proline, chlorophyll are the relevant substance indexs of seedling salt tolerance, these projects reflect corn from different angles
The salt tolerance of Seedling Stage is strong and weak.
When carrying out Maize at Seedling Stage salt tolerance metrics evaluation, the germination examination of corn seed salt stress usually is carried out in laboratory
It tests, large seed germination test generally uses casting bed (fine sand of 0.05-0.8mm) to be cultivated, and the casting bed salt side of body is established in test
The method for the condition of compeling is the addition respective concentration salting liquid into casting bed, but with the sprouting of seed, emergence, growth and environment
The salt concentration of the variation of factor, casting bed can change, and cause accurately obtain certain constant salt stress concentration in test
Test data, so probing into salt content in casting bed maintains salt stress concentration item to establish with the changing rule of germination
A stable system of part, for Maize at Seedling Stage Salt-Tolerance Identification and evaluation, the in-depth analysis of Maize at Seedling Stage Mechanism of Salt-tolerant has weight
Big effect.
By retrieval, following two patent publication us relevant to present patent application are found:
1, a kind of characterization and evaluation method (CN108966729A) of Maize at Seedling Stage salt tolerance, the identification method, use with
Lower step: (1) seed cleaning, disinfection and nursery, seedling were cultivated to one heart stage of three leaves；(2) by growing way consistent one heart stage of three leaves
Seedling is divided into two parts, and portion is test group, and portion is control group；Contain 150-200mmol/LNaCl in test group nutrient solution；
(3) plant moisture is blotted with blotting paper, measures the chlorophyll content (C) of the third piece leaf of test group and control group；Measurement from
Root is denoted as plant height (PH) to the extreme length of blade tip；The length for measuring longest root, is denoted as root long (RH)；Record root or more
Fresh weight, be denoted as leaf fresh weight (YF) and root fresh weight, be denoted as root fresh weight (GF)；Leaf portion and root are handled in 120 DEG C afterwards
20min, 80 DEG C of processing 48h weigh dry weight, are denoted as leaf dry weight (YD) and root dry weight (GD) respectively；(4) by above-mentioned numerical value substitute into
Lower formula, calculates the salt tolerant efficiency of each character: salt tolerant efficiency=test group measured value/control group measured value.This method improves logical
Amount realizes accurate test, shortens the test period, improves working efficiency, data, accurate evaluation salt tolerance is effectively treated.
2, a kind of method (CN102524054A) for screening salt tolerance transgenic corns, comprising the following steps: (1) maize seed
Son sprouts, corn seed sowing in (2) greenhouse, the processing of (3) plant salt stress, (4) maize leaf physiological and biochemical index
Detection, the evaluation of (5) corn salt resistance ability and (6) selection have the transgenic corns of salt tolerance.Method of the invention establishes one
The method of a suitable Maize at Seedling Stage Evaluation of Salt Tolerance, the system pour place using corn seedling as test object, with sodium chloride solution
After reason, by the detection to plant physiological and biochemical index, reaches and establish an objective and accurate transgenic corns Evaluation of Salt Tolerance
The purpose of method, so that the screening for salt tolerance transgenic corns provides reliable foundation.
Early period of the invention screens through a large number of experiments, identifies more parts of corn inbred lines, and system summary has obtained 8 ‰
NaCl mass concentration is preferably to evaluate the most suitable salinity of corn inbred line, under the concentration, by being referred to dry weight change rate
Mark can accurately evaluate the salt tolerance of corn inbred line, provide for the development that practical study works more accurate and convenient and fast
Operating method, this is the main feature of invention.
Therefore, there is essential difference by comparison, present patent application and above-mentioned patent publication us.
Summary of the invention
It is an object of the invention in place of overcome the deficiencies in the prior art, provide a kind of efficient Maize at Seedling Stage salt tolerance to comment
Valence method, this method use constant salinity, realize the salt tolerant performance that quick, precise Identification goes out corn, establish constant salt
The identification of Maize Seedling salt tolerance, assessment technique system under concentration, efficiently produce corn and corn variety breeding has
Important meaning.
The present invention solves its technical problem and adopts the following technical solutions to achieve:
A kind of efficient Maize at Seedling Stage salt endurance appraisement method, steps are as follows:
(1) determine to use when corn seedling Salt-Tolerance Identification and be carried out under constant 8 ‰ NaCl mass concentration；
(2) it is fresh that the vitality index of the corn seed under the processing of constant 8 ‰ NaCl mass concentration, plant height, root long, plant are measured
Weight, overground part dry weight, underground part dry weight, leaf chlorophyll and proline content, blade and root K+/Na+ ratio；
(3) determine dry weight change rate (%) index, and determine dry weight change rate (%) index and corn germplasm salt tolerance it
Between evaluation and test parameter, evaluate Maize at Seedling Stage salt tolerance.
Moreover, the step (1) in determine corn seedling Salt-Tolerance Identification when using constant 8 ‰ NaCl mass concentration when
Method are as follows:
Using indoor casting bed cultivation, the totally 5 various concentration gradients of control 0,2 ‰, 4 ‰, 6 ‰, 8 ‰ are respectively set
The Stress treatment of NaCl solution, if 3 repetitions；It is carried out using 12cm × 12cm × 5cm germination box, in each germination box respectively
The casting bed for preparing 3cm high, being poured with various concentration NaCl solution sows each 25；It is subsequently placed in growth cabinet, 25 DEG C of trainings
It supports.
Moreover, the step (2) in the processing of constant 8 ‰ NaCl mass concentration are as follows:
It is carried out using 12cm × 12cm × 5cm germination box, prepares 3cm high respectively in each germination box, uses various concentration
The casting bed that NaCl solution is poured sows each 25；It is subsequently placed in growth cabinet, 25 DEG C of cultures.
Moreover, the step (3) in using to Maize at Seedling Stage Harmful salt be subordinate to letter when determining dry weight change rate (%) index
Numerical value, the Maize at Seedling Stage Harmful salt membership function value include germination percentage, root/shoot ratio, relative water content (%), salt gypsum rock
(%)；Wherein total 100/ seed of the germinative number * sum of germination percentage (%)=seven day seed, root/shoot ratio=root dry weight/seedling stem weight, relatively
Water content (%)=(fresh weight-dry weight) * 100/ fresh weight, salt gypsum rock (%)=(control group-processing group) * 100/ control group.
Moreover, the step (3) in dry weight change rate (%)=[(control-processing)/control] × 100%.
Moreover, the evaluation and test parameter of the dry weight change rate (%) and corn germplasm material salt tolerance are as follows: I height resistance to 0~20；Ⅱ
Salt tolerant 20.1~40；Resistance to 40.1~60 in III；IV sensitivity 60.1~80；V high sense 80.1~100.
And, the specific steps are as follows:
(1) the HgCl of corn seed mass concentration 0.1%2Solution disinfection 15min, and it is clean with distilled water flushing, it is standby
With；
(2), using indoor casting bed cultivation, carried out in 12cm × 12cm × 5cm germination box, it is quasi- respectively in each germination box
Standby 3cm high, the casting bed poured with various concentration NaCl solution, sow each 25, are placed in growth cabinet, 25 DEG C of cultures；
(3) in seed germination, with NaCl make NaCl mass concentration be 8 ‰ Hoagland nutrient solution and
The aqueous solution that NaCl mass concentration is 8 ‰, in each germination box, the same day first adds the prepared with 8 ‰ NaCl of equivalent
According to germination box institute weight loss, the distilled water of identical weight is added on the 3rd, 7,11,13 and 17 day in Hoagland nutrient solution；The 5th
The configured 8 ‰ NaCl aqueous solution of identical weight is added according to germination box institute weight loss in it；9th day and the 15th day according to hair
The Hoagland nutrient solution containing 8 ‰ NaCl of identical weight is added in bud box institute weight loss, maintains salt tolerance to evaluate stable salt dense
Degree；
(4) after constant 8 ‰ NaCl Stress treatment seven days, intact plant is taken out, gets fine sand and ash express developed with deionized water
Dirt blots plant deionized water remained on surface with blotting paper, and plant is then put into baking oven with 105 DEG C of water-removing 10min, and 80 DEG C
Drying to constant weight, then weighs, by formula: dry weight change rate (%)=[(control-processing)/control] × 100% calculates dry
Weight change rate；
(5) the salt tolerance grade of each corn variety or strain is determined:
The relationship of different stage salt tolerance and dry weight change rate (%): I height resistance to 0~20；II salt tolerant 20.1~40；It is resistance in III
40.1~60；IV sensitivity 60.1~80；V high sense 80.1~100.
The advantages of present invention obtains and good effect are:
1, the method for the present invention is using constant 8 ‰ NaCl mass concentration as corn germplasm material Seedling Salt-tolerance screening and assessment
Optimal concentration, select dry weight change rate as evaluation and test main indicator, convenient for accurately measuring the difference between different materials, into
The Evaluation of Salt Tolerance of early stage can be carried out when row maize culture and breeding of new variety indoors, improves the efficient of actual production work
Property.
2, this method is using the sand matrix of constant salinity and the salt tolerance of dry weight change rate (%) index and corn germplasm
Between evaluation and test parameter, realize quickly, precise Identification go out corn salt tolerant performance, establish Maize Seedling under constant salinity
The identification of salt tolerance, assessment technique system, efficiently produce corn and corn variety breeding has great importance.
3, the method for the present invention includes these steps: the preparation of chiltern germinating bed；Seed preparation；The NaCl of adjusting 0.8% is dense
Degree；Germination test maintains 0.8% constant salinity therebetween；The index of correlation of Maize Seedling Emergence Stage is measured, identification, evaluation are beautiful
The salt tolerance of rice.The method of seedling stage assay salt tolerance of corn of the invention is realized fast using the sand matrix of constant salinity
Speed, precise Identification go out the salt tolerant performance of corn, establish the identification of Maize Seedling salt tolerance under constant salinity, assessment technique body
System.This system is showed in conjunction with the yield of salt pond planting experiment, determines constant 0.8% salinity, the root long of corn seedling,
Root fresh weight, root dry weight, seedling fresh weight and seedling stem are the characterization and evaluation index of effective salt tolerance of corn again.Present invention can be implemented in seedlings
Phase carries out efficiently and accurately Salt-Tolerance Identification and evaluation to corn variety and germ plasm resource.
4, the system stable using maintenance salt stress concentration conditions established by the present invention, carries out the jade of different NaCl concentrations
Rice seed germination experiment determines the optimal limit concentration of different corn variety Seedling Salt-tolerance screenings.Salt is carried out using this concentration
Germination test is coerced, preliminary screening is carried out to salt tolerance of corn, and analyze the correlation of each index salt damage coefficient of Maize at Seedling Stage,
Grading evaluation is carried out to salt tolerance of corn, establishes a set of Maize at Seedling Stage Evaluation of Salt Tolerance system.
Detailed description of the invention
Fig. 1 is a kind of Maize at Seedling Stage Efficient salt-tolerant identity process figure in the present invention.
Specific embodiment
Below with reference to the invention will be further described by specific embodiment, following embodiment be it is descriptive, no
It is restrictive, this does not limit the scope of protection of the present invention.
Raw material used in the present invention is unless otherwise specified conventional commercial product；Used in the present invention
Method is unless otherwise specified the conventional method of this field.
A kind of efficient Maize at Seedling Stage salt endurance appraisement method, steps are as follows:
(1) determine to use when corn seedling Salt-Tolerance Identification and be carried out under constant 8 ‰ NaCl mass concentration；
(2) it is fresh that the vitality index of the corn seed under the processing of constant 8 ‰ NaCl mass concentration, plant height, root long, plant are measured
Weight, overground part dry weight, underground part dry weight, leaf chlorophyll and proline content, blade and root K+/Na+ ratio；
(3) determine dry weight change rate (%) index, and determine dry weight change rate (%) index and corn germplasm salt tolerance it
Between evaluation and test parameter, evaluate Maize at Seedling Stage salt tolerance.
More preferably, the step (1) in determine corn seedling Salt-Tolerance Identification when use constant 8 ‰ NaCl mass concentration when
Method are as follows:
Using indoor casting bed cultivation, the totally 5 various concentration gradients of control 0,2 ‰, 4 ‰, 6 ‰, 8 ‰ are respectively set
The Stress treatment of NaCl solution, if 3 repetitions；It is carried out using 12cm × 12cm × 5cm germination box, in each germination box respectively
The casting bed for preparing 3cm high, being poured with various concentration NaCl solution sows each 25；It is subsequently placed in growth cabinet, 25 DEG C of trainings
It supports.
More preferably, the step (2) in the processing of constant 8 ‰ NaCl mass concentration are as follows:
It is carried out using 12cm × 12cm × 5cm germination box, prepares 3cm high respectively in each germination box, uses various concentration
The casting bed that NaCl solution is poured sows each 25；It is subsequently placed in growth cabinet, 25 DEG C of cultures.
More preferably, the step (3) in determine dry weight change rate (%) index when be subordinate to using to Maize at Seedling Stage Harmful salt
Functional value, the Maize at Seedling Stage Harmful salt membership function value include germination percentage, root/shoot ratio, relative water content (%), salt gypsum rock
(%)；Wherein total 100/ seed of the germinative number * sum of germination percentage (%)=seven day seed, root/shoot ratio=root dry weight/seedling stem weight, relatively
Water content (%)=(fresh weight-dry weight) * 100/ fresh weight, salt gypsum rock (%)=(control group-processing group) * 100/ control group.
More preferably, the step (3) in dry weight change rate (%)=[(control-processing)/control] × 100%.
More preferably, the evaluation and test parameter of the dry weight change rate (%) and corn germplasm material salt tolerance are as follows: I height resistance to 0~20；
II salt tolerant 20.1~40；Resistance to 40.1~60 in III；IV sensitivity 60.1~80；V high sense 80.1~100.
More preferably, as shown in Figure 1, the specific steps are as follows:
(1) the HgCl of corn seed mass concentration 0.1%2Solution disinfection 15min, and it is clean with distilled water flushing, it is standby
With；
(2), using indoor casting bed cultivation, carried out in 12cm × 12cm × 5cm germination box, it is quasi- respectively in each germination box
Standby 3cm high, the casting bed poured with various concentration NaCl solution, sow each 25, are placed in growth cabinet, 25 DEG C of cultures；
(3) in seed germination, with NaCl make NaCl mass concentration be 8 ‰ Hoagland nutrient solution and
The aqueous solution that NaCl mass concentration is 8 ‰, in each germination box, the same day first adds the prepared with 8 ‰ NaCl of equivalent
According to germination box institute weight loss, the distilled water of identical weight is added on the 3rd, 7,11,13 and 17 day in Hoagland nutrient solution；The 5th
The configured 8 ‰ NaCl aqueous solution of identical weight is added according to germination box institute weight loss in it；9th day and the 15th day according to hair
The Hoagland nutrient solution containing 8 ‰ NaCl of identical weight is added in bud box institute weight loss, maintains salt tolerance to evaluate stable salt dense
Degree；
(4) after constant 8 ‰ NaCl Stress treatment seven days, intact plant is taken out, gets fine sand and ash express developed with deionized water
Dirt blots plant deionized water remained on surface with blotting paper, and plant is then put into baking oven with 105 DEG C of water-removing 10min, and 80 DEG C
Drying to constant weight, then weighs, by formula: dry weight change rate (%)=[(control-processing)/control] × 100% calculates dry
Weight change rate；
(5) the salt tolerance grade of each corn variety or strain is determined:
The relationship of different stage salt tolerance and dry weight change rate (%): I height resistance to 0~20；II salt tolerant 20.1~40；It is resistance in III
40.1~60；IV sensitivity 60.1~80；V high sense 80.1~100.
Specifically, the preparation of Hoagland nutrient solution can be such that
A great number of elements: KNO3、KH2PO4、Ca(NO3)2、MgSO4·7H2O, it is formulated as the mother liquor of 1M respectively.
The preparation of EDTA-Fe: 2.68g sodium ethylene diamine tetracetate (Na is weighed2C10H14O3N2) be placed in 1000mL distilled water
It dissolves by heating, 1.980gFeSO is added while hot4·7H2O is stirred evenly.
Microelement: 2.860g H is weighed respectively3BO3、0.222g ZnSO4·7H2O、0.025g NaMoO4、2.110g
MnSO4·4H2O、0.079g CuSO4·5H2O is fully dissolved in 800mL distilled water, is settled to 1000mL.
Complete culture solution configuration: measuring 800mL distilled water, and according to the form below is separately added into each stock solution and is configured to complete nutrition
Liquid, and pH is adjusted in 5.6-6.0 with 0.1MNaOH or 0.1MHCl, it is settled to 1000mL.
More specifically, efficient Maize at Seedling Stage salt endurance appraisement method, steps are as follows:
Constant 8 ‰ NaCl concentration control method:
Selection purpose corn material does germination test with sandy culture indoors as shown in table 1, respectively with 0 (CK), 8 ‰
(I) the NaCl-Hoagland nutrient solution of 2 concentration carries out salt stress processing, sets 3 repetitions respectively.The addition pair in germination box
The sand for answering the NaCl solution 120mL and 600g of concentration to sterilize stirs evenly, and randomly selects the net seed of each corn, is with concentration
0.1%HgCl2Sterilize 15min, then by seed be placed in germination box in, 25 DEG C in growth cabinet, 70%RH culture.In the 20th
Its sampling, measures a variety of physiological and biochemical indexs, the results are shown in Table 2.
Measure corn seedling dry weight change rate:
After constant 8 ‰ NaCl Stress treatment seven days, intact plant is taken out, gets fine sand and ash express developed with deionized water
Dirt blots plant deionized water remained on surface with blotting paper, records fresh weight.Then plant baking oven is put into finish with 105 DEG C
10min, 80 DEG C drying to constant weight, then weighs, by formula: dry weight change rate (%)=[(control-processing)/control] ×
100%, calculate dry weight change rate.
The measurement of salt damage coefficient:
For each testing index, the salt damage coefficient of the corn germplasm is evaluated, specific formula for calculation: salt gypsum rock (%)=
(control group-processing group) * 100/ control group, the results are shown in Table 3.
The corn material of the progress Salt-Tolerance Identification of table 1
2 NaCl of table coerces the membership function value of lower each index salt damage coefficient of Maize Seedlings
Lowercase alphabet shows between same index different materials in note table has significant difference in the level of P < 5%.
3 NaCl of table coerces lower each index salt damage Relativity of Coefficients of Maize Seedlings
Note:*Indicate 0.05 horizontal correlation,**Indicate 0.01 horizontal correlation.
Note:“*”is significant at the 0.05level(2-tailed).“**”is significant
at the 0.01level(2-tailed)。
It can be seen that sand matrix and dry weight change rate (%) index and maize seed of this method method using constant salinity
Evaluation and test parameter between the salt tolerance of matter may be implemented the salt tolerant performance that quick, precise Identification goes out corn, it is dense to establish constant salt
Identification, the assessment technique system for spending lower Maize Seedling salt tolerance, efficiently produce corn and corn variety breeding have weight
The meaning wanted.
Although disclosing the embodiment of the present invention for the purpose of illustration, it will be appreciated by those skilled in the art that: not
Be detached from the present invention and spirit and scope of the appended claims in, various substitutions, changes and modifications be all it is possible, therefore, this
The range of invention is not limited to the embodiment disclosure of that.
Claims (7)
1. a kind of efficient Maize at Seedling Stage salt endurance appraisement method, it is characterised in that: steps are as follows:
(1) determine to use when corn seedling Salt-Tolerance Identification and be carried out under constant 8 ‰ NaCl mass concentration；
(2) measure the vitality index of the corn seed under the processing of constant 8 ‰ NaCl mass concentration, plant height, root long, plant fresh weight,
Top dry weight, underground part dry weight, leaf chlorophyll and proline content, blade and root K+/Na+ ratio；
(3) determine dry weight change rate (%) index, and determine between dry weight change rate (%) index and the salt tolerance of corn germplasm
Parameter is evaluated and tested, Maize at Seedling Stage salt tolerance is evaluated.
2. efficient Maize at Seedling Stage salt endurance appraisement method according to claim 1, it is characterised in that: the step (1) in
Method when determining corn seedling Salt-Tolerance Identification using constant 8 ‰ NaCl mass concentration are as follows:
Using indoor casting bed cultivation, the NaCl that the totally 5 various concentration gradients of control 0,2 ‰, 4 ‰, 6 ‰, 8 ‰ are respectively set is molten
The Stress treatment of liquid, if 3 repetitions；It is carried out using 12cm × 12cm × 5cm germination box, is prepared respectively in each germination box
3cm high, the casting bed poured with various concentration NaCl solution, sow each 25；It is subsequently placed in growth cabinet, 25 DEG C of cultures.
3. efficient Maize at Seedling Stage salt endurance appraisement method according to claim 1, it is characterised in that: the step (2) in
Constant 8 ‰ NaCl mass concentration processing are as follows:
It is carried out using 12cm × 12cm × 5cm germination box, prepares 3cm high in each germination box respectively, with various concentration NaCl
The casting bed that solution pours sows each 25；It is subsequently placed in growth cabinet, 25 DEG C of cultures.
4. efficient Maize at Seedling Stage salt endurance appraisement method according to claim 1, it is characterised in that: the step (3) in
Use is subordinate to Maize at Seedling Stage Harmful salt membership function value, the Maize at Seedling Stage Harmful salt when determining dry weight change rate (%) index
Membership fuction value includes germination percentage, root/shoot ratio, relative water content (%), salt gypsum rock (%)；Wherein germination percentage (%)=seven day kind
Sub 100/ seed sum of total germinative number *, root/shoot ratio=root dry weight/seedling stem weight, relative water content (%)=(fresh weight-dry weight) *
100/ fresh weight, salt gypsum rock (%)=(control group-processing group) * 100/ control group.
5. the efficient Maize at Seedling Stage salt endurance appraisement method according to right 1, it is characterised in that: the step (3) in dry weight
Change rate (%)=[(control-processing)/control] × 100%.
6. efficient Maize at Seedling Stage salt endurance appraisement method according to any one of claims 1 to 5, it is characterised in that: institute
State the evaluation and test parameter of dry weight change rate (%) Yu corn germplasm material salt tolerance are as follows: I height resistance to 0~20；II salt tolerant 20.1~40；Ⅲ
In resistance to 40.1~60；IV sensitivity 60.1~80；V high sense 80.1~100.
7. efficient Maize at Seedling Stage salt endurance appraisement method according to claim 1, it is characterised in that: specific steps are such as
Under:
(1) the HgCl of corn seed mass concentration 0.1%2Solution disinfection 15min, and it is clean with distilled water flushing, it is spare；
(2), using indoor casting bed cultivation, carry out in 12cm × 12cm × 5cm germination box, prepare respectively in each germination box
3cm high, the casting bed poured with various concentration NaCl solution, sow each 25, are placed in growth cabinet, 25 DEG C of cultures；
(3) in seed germination, Hoagland nutrient solution and the NaCl matter that NaCl mass concentration is 8 ‰ are made with NaCl
Measure the aqueous solution that concentration is 8 ‰, in each germination box, the same day first add equivalent with the prepared Hoagland nutrition of 8 ‰ NaCl
According to germination box institute weight loss, the distilled water of identical weight is added on the 3rd, 7,11,13 and 17 day in liquid；At the 5th day according to germination box
The configured 8 ‰ NaCl aqueous solution of identical weight is added in institute's weight loss；9th day and the 15th day according to germination box institute weight loss,
The Hoagland nutrient solution containing 8 ‰ NaCl of identical weight is added, salt tolerance is maintained to evaluate stable salinity；
(4) after constant 8 ‰ NaCl Stress treatment seven days, intact plant is taken out, gets fine sand and dust express developed with deionized water,
Plant deionized water remained on surface is blotted with blotting paper, plant is then put into baking oven with 105 DEG C of water-removing 10min, 80 DEG C of bakings
It does to constant weight, then weighs, by formula: dry weight change rate (%)=[(control-processing)/control] × 100% calculates dry weight
Change rate；
(5) the salt tolerance grade of each corn variety or strain is determined:
The relationship of different stage salt tolerance and dry weight change rate (%): I height resistance to 0~20；II salt tolerant 20.1~40；Resistance to 40.1 in III
~60；IV sensitivity 60.1~80；V high sense 80.1~100.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111869359A (en) * | 2020-08-13 | 2020-11-03 | 天津农学院 | Soil salinity gradient adjusting method suitable for physiological research under corn salt stress |
| CN111869368A (en) * | 2020-08-13 | 2020-11-03 | 天津农学院 | Rapid seedling stage evaluation method for salt tolerance of rape seeds |
| CN112470896A (en) * | 2019-09-10 | 2021-03-12 | 山东农业大学 | Method for identifying salt tolerance of peanut in seedling stage |
| CN113841536A (en) * | 2021-09-24 | 2021-12-28 | 上海市农业科学院 | Method for detecting flooding-resistant strength and weakness degree of fresh corn and application thereof |
| CN115119734A (en) * | 2022-07-25 | 2022-09-30 | 河北省农林科学院滨海农业研究所 | Method for rapidly identifying salt tolerance of plants |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1296058A1 (en) * | 1985-09-23 | 1987-03-15 | Ростовский государственный университет им.М.А.Суслова | Method of estimating salt-endurance of sunflower species |
| CN104521373A (en) * | 2015-02-03 | 2015-04-22 | 天津农学院 | Control method of constant salt concentration in sand matrix for maize germination test |
-
2019
- 2019-04-18 CN CN201910311524.5A patent/CN110024524A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1296058A1 (en) * | 1985-09-23 | 1987-03-15 | Ростовский государственный университет им.М.А.Суслова | Method of estimating salt-endurance of sunflower species |
| CN104521373A (en) * | 2015-02-03 | 2015-04-22 | 天津农学院 | Control method of constant salt concentration in sand matrix for maize germination test |
Non-Patent Citations (2)
| Title |
|---|
| 吴翠萍等: "玉米幼苗期耐盐性评价的研究", 《天津农学院学报》 * |
| 罗敏等: "玉米幼苗期耐盐性评价适宜盐浓度的研究", 《天津农学院学报》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112470896A (en) * | 2019-09-10 | 2021-03-12 | 山东农业大学 | Method for identifying salt tolerance of peanut in seedling stage |
| CN111869359A (en) * | 2020-08-13 | 2020-11-03 | 天津农学院 | Soil salinity gradient adjusting method suitable for physiological research under corn salt stress |
| CN111869368A (en) * | 2020-08-13 | 2020-11-03 | 天津农学院 | Rapid seedling stage evaluation method for salt tolerance of rape seeds |
| CN113841536A (en) * | 2021-09-24 | 2021-12-28 | 上海市农业科学院 | Method for detecting flooding-resistant strength and weakness degree of fresh corn and application thereof |
| CN115119734A (en) * | 2022-07-25 | 2022-09-30 | 河北省农林科学院滨海农业研究所 | Method for rapidly identifying salt tolerance of plants |
| CN115119734B (en) * | 2022-07-25 | 2023-08-11 | 河北省农林科学院滨海农业研究所 | Method for rapidly identifying salt tolerance of plants |
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