CN105210861B - A kind of cotton seeds selection of salt tolerance method - Google Patents
A kind of cotton seeds selection of salt tolerance method Download PDFInfo
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Abstract
The invention discloses a kind of cotton seeds selection of salt tolerance method, comprise the following steps：After cotton seeds are handled, with the artificial seawater nutrient solution of various concentrations, filtered after seed soaking；It is placed in culture dish, plus artificial seawater nutrient solution, germinative number is recorded daily, and calculates germinating energy, germination index and vitality index；The root of cotton seeds after germination is clean with distilled water flushing, and point individual plant is moved into be cultivated in the shading-glass bottle of the artificial seawater nutrient solution equipped with various concentrations, and regularly supplements corresponding artificial seawater nutrient solution daily into the whole root immersion nutrient solutions of cotton seedling；Take out the fibrous root quantity for checking every plant of cotton within 8th 11 day；The thick Seedling Height of each treatment group, stem number, stem, root Fresh Yuxincao, Root dry weight and root volume are determined after 20d and carries out data and calculates assessment.The present invention is by ciltivating process, to its sprouting stage and seedling stage economical character index multi-variate statistical analysis, so that the salt tolerance to cotton has entered comprehensive identification evaluation.
Description
Technical field
The present invention relates to plant physiology field, and in particular to a kind of cotton seeds selection of salt tolerance method.
Background technology
The salt tolerance of cotton not only stablizes relevant relatively with osmotic adjustment ability and Protective substances, more depends primarily on
Selection of the root system to salinity absorbs and its in the compartmentalization of organ, three levels of tissue and cell.If the salt ion energy absorbed
It is more to stay in root, the transport and distribution to overground part are reduced, then can show stronger Salt-endurance.Simultaneously because the salt tolerant of plant
Alkalescence is more complicated by controlled by multiple genes, resistance mechanism, and root system is that cotton is aggrieved earliest, most direct in saline and alkaline adverse circumstance
Position, so the fresh weight of root system, dry weight, root length, radical are all important morphological indexs in Salt And Alkali Tolerance identification, meanwhile, coring
Change of the quantity to salt concentration is most sensitive, therefore it can be adapted to this as Salt And Alkali Tolerance cotton early stage identification system and screening
The ideal indicator of the early stage identification of system.
For a long time, the screening or identification of confrontation/Salt And Alkali Tolerance cotton material, is to use field salt-soda soil, artificial salt pond
Tested, or indoor measurement some Physiology and biochemistries parameter, be used as comparison reference.Existing method is in salt according to cotton material
Alkali or salt pond in upgrowth situation carry out salt damage and check to be judged whether that there is anti-/ patience to saline and alkaline.Although this method is
Directly in field test, qualification result is relatively direct, but the annual cotton only one of which season of growth, i.e., can only carry out every year
A collection of experiment；To higher required by verification field, as salinity will have certain gradient, salt pond or salt-soda soil place can not be changed,
Because the salt-soda soil or salt pond by this kind of qualification test are not available to plant other plant, to prevent bringing salt to other crops
Evil, floor space is big, and determination rates are low；Salt damage symptom is individual qualitatively scope, without specific digital quantization, different calibratings
The result difference of personal observations is larger, and the conclusion drawn has difference and unstable；Field salt-soda soil is nature, and soil
The degree of mixedness and saline and alkaline of earth are difficult unification, and although the salinity in salt pond is the NaCl being manually sprinkled into, but enter after soil, soil
The factor such as composition, pH value and weather rainfall can cause the change at any time of salinity level, test knot between year and between parallel
The comparativity of fruit is poor.
Water planting is one kind of soilless culture, is a new and high technology in modern agriculture science and technology, because it is with the root of artificial creation
Based environment instead of soil environment, because it has many advantages in Tolerant salt, and especially it provides a homogeneous root system
Ecological environment, thus reaction of the plant to salt damage can be gone out with Accurate Determining, so having obtained extensively should in plant salt tolerance identification
With.
The content of the invention
To solve the above problems, the invention provides a kind of cotton seeds selection of salt tolerance method, it is right by ciltivating process
Its sprouting stage and seedling stage economical character index multi-variate statistical analysis, so that the salt tolerance to cotton has entered comprehensive identification evaluation.
To achieve the above object, the technical scheme taken of the present invention is：
A kind of cotton seeds selection of salt tolerance method, comprises the following steps：
S1, selection maturity and cotton seeds of the same size, except mould impurity elimination, attachment seed is killed using carbendazim spraying
The bulbil and mycelia on surface, distilled water flushing 3 times；Using 1/2Hoagland complete nutritions liquid as solution, mixed with artificial seawater
0.0wt%, 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt% and 0.8wt% are configured to respectively
Filtered after artificial seawater nutrient solution, seed soaking 48h；It is placed in and is covered with the culture dish of 2 metafiltration paper, adds artificial seawater nutrient solution per ware
10mL, in vernalization at 28 DEG C, records germinative number daily, and calculates germinating energy, germination index, cleans seed and changes artificial seawater
Nutrient solution；
Meanwhile, plumular axis is determined using glass vertical panel sproutung method and radicle is long, vitality index is calculated；Each processing repeats 3
It is secondary,
It is S2, the root of the cotton seeds after germination is clean with distilled water flushing, point individual plant move into equipped with 0.0wt%,
The screening of 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt% and 0.8wt% artificial seawater nutrient solution
In light vial, cotton cotyledon is stayed in outside bottleneck, is placed in 23 ± 2 DEG C, light intensity 2000lx, is trained under the conditions of photoperiod 12/12h
Support, regularly supplement corresponding artificial seawater nutrient solution in vial daily into the whole root immersion nutrient solutions of cotton seedling；
The radical amount of every plant of cotton, including the fibrous root quantity in taproot and lateral root and lateral root are checked in S3, taking-up in the 8-11 days；
After S4, processing 20d, determine respectively the thick Seedling Height of each treatment group, stem number, stem, root Fresh Yuxincao, Root dry weight and
Root volume；
S5, data preparation, calculate average value, and according to the salt tolerant efficiency α of each single index of below equation calculating：
α=treatment group measured value/control group measured value
The synthesis D of resistance to salt figure of each kind is calculated according to the following formula：
D=X1*0.903+X2*0.906+X3*0.789+X4*0.961+X5*0.621+X6*0.831+ X7*0.643+X8*
0.958+X9*0.945+X10*0.935+X11*0.906
Wherein, X1, X2, X3, X4, X5, X6, X7, X8, X9, X10, X11 represent germinating energy, germination index, coring number respectively
Amount, radicle length, plant height, stem number, stem thick, the salt tolerant efficiency of vitality index, root Fresh Yuxincao, Root dry weight and root volume；
S6, the salt tolerance of kind judged according to the size of comprehensive resistance to salt figure, D values are bigger, and the salt damage being subject to is smaller, kind
Salt tolerance it is stronger；D values are smaller, and the salt damage being subject to is bigger, and the salt tolerance of kind is weaker.
Wherein, every liter of the artificial seawater includes 26.726 grams of sodium chloride, 2.26 grams of magnesium chloride, 3.248 grams of magnesium sulfate, chlorine
Change 1.153 grams of calcium, 0.198 gram of sodium acid carbonate, 0.721 gram of potassium chloride.
The invention has the advantages that：
By using ciltivating process culture cotton plants, field sets different salinity gradients in nutrient solution, by specific
Seedling raising mannerses and the transplanting of specific time, and check specific parameter in the specific time, according to it is specific, can directly observe
, simple parameter judge the resistance level of the cotton material, while polynary to its sprouting stage and seedling stage economical character index
Statistical analysis, so that the salt tolerance to cotton has entered comprehensive identification evaluation.
Embodiment
In order that objects and advantages of the present invention are more clearly understood, the present invention is carried out with reference to embodiments further
Describe in detail.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit this hair
It is bright.
Every liter of artificial seawater used in following examples includes 26.726 grams of sodium chloride, 2.26 grams of magnesium chloride, sulfuric acid
3.248 grams of magnesium, 1.153 grams of calcium chloride, 0.198 gram of sodium acid carbonate, 0.721 gram of potassium chloride.
Embodiment
S1, selection maturity and cotton seeds of the same size, except mould impurity elimination, attachment seed is killed using carbendazim spraying
The bulbil and mycelia on surface, distilled water flushing 3 times；Using 1/2Hoagland complete nutritions liquid as solution, mixed with artificial seawater
0.0wt%, 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt% and 0.8wt% are configured to respectively
Filtered after artificial seawater nutrient solution, seed soaking 48h；It is placed in and is covered with the culture dish of 2 metafiltration paper, adds artificial seawater nutrient solution per ware
10mL, in vernalization at 28 DEG C, records germinative number daily, and calculates germinating energy, germination index, cleans seed and changes artificial seawater
Nutrient solution；
Meanwhile, plumular axis is determined using glass vertical panel sproutung method and radicle is long, vitality index is calculated；Each processing repeats 3
It is secondary,
It is S2, the root of the cotton seeds after germination is clean with distilled water flushing, point individual plant move into equipped with 0.0wt%,
The screening of 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt% and 0.8wt% artificial seawater nutrient solution
In light vial, cotton cotyledon is stayed in outside bottleneck, is placed in 23 ± 2 DEG C, light intensity 2000lx, is trained under the conditions of photoperiod 12/12h
Support, regularly supplement corresponding artificial seawater nutrient solution in vial daily into the whole root immersion nutrient solutions of cotton seedling；
The radical amount of every plant of cotton, including the fibrous root quantity in taproot and lateral root and lateral root are checked in S3, taking-up in the 8-11 days；
After S4, processing 20d, determine respectively the thick Seedling Height of each treatment group, stem number, stem, root Fresh Yuxincao, Root dry weight and
Root volume；
S5, data preparation, calculate average value, and according to the salt tolerant efficiency α of each single index of below equation calculating：
α=treatment group measured value/control group measured value
The synthesis D of resistance to salt figure of each kind is calculated according to the following formula：
D=X1*0.903+X2*0.906+X3*0.789+X4*0.961+X5*0.621+X6*0.831+ X7*0.643+X8*
0.958+X9*0.945+X10*0.935+X11*0.906
Wherein, X1, X2, X3, X4, X5, X6, X7, X8, X9, X10, X11 represent germinating energy, germination index, coring number respectively
Amount, radicle length, plant height, stem number, stem thick, the salt tolerant efficiency of vitality index, root Fresh Yuxincao, Root dry weight and root volume；
S6, the salt tolerance of kind judged according to the size of comprehensive resistance to salt figure, D values are bigger, and the salt damage being subject to is smaller, kind
Salt tolerance it is stronger；D values are smaller, and the salt damage being subject to is bigger, and the salt tolerance of kind is weaker.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (2)
1. a kind of cotton seeds selection of salt tolerance method, it is characterised in that comprise the following steps：
S1, selection maturity and cotton seeds of the same size, except mould impurity elimination, attachment the surface of the seed is killed using carbendazim spraying
Bulbil and mycelia, distilled water flushing 3 times；Using 1/2Hoagland complete nutritions liquid as solution, difference is mixed with artificial seawater
It is configured to the artificial of 0.0wt%, 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt% and 0.8wt%
Filtered after seawater nutrient solution, seed soaking 48h；It is placed in and is covered with the culture dish of 2 metafiltration paper, adds artificial seawater nutrient solution 10mL per ware,
In vernalization at 28 DEG C, germinative number is recorded daily, and calculates germinating energy, germination index, is cleaned seed and is changed artificial seawater nutrition
Liquid；
Meanwhile, plumular axis is determined using glass vertical panel sproutung method and radicle is long, vitality index is calculated；Each processing is repeated 3 times,
It is S2, the root of the cotton seeds after germination is clean with distilled water flushing, point individual plant move into equipped with 0.0wt%, 0.1wt%,
The shading-glass bottle of 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt% and 0.8wt% artificial seawater nutrient solution
Interior, cotton cotyledon is stayed in outside bottleneck, is placed in 23 ± 2 DEG C, light intensity 2000lx, is cultivated under the conditions of photoperiod 12/12h, glass
Corresponding artificial seawater nutrient solution is regularly supplemented in bottle daily into the whole root immersion nutrient solutions of cotton seedling；
The radical amount of every plant of cotton, including the fibrous root quantity in taproot and lateral root and lateral root are checked in S3, taking-up in the 8-11 days；
After S4, processing 20d, the thick Seedling Height of each treatment group, stem number, stem, root Fresh Yuxincao, Root dry weight and root body are determined respectively
Product；
S5, data preparation, calculate average value, and according to the salt tolerant efficiency α of each single index of below equation calculating：
α=treatment group measured value/control group measured value
The synthesis D of resistance to salt figure of each kind is calculated according to the following formula：
D=X1*0.903+X2*0.906+X3*0.789+X4*0.961+X5*0.621+X6*0.831+ X7*0.643+X8*
0.958+X9*0.945+X10*0.935+X11*0.906
Wherein, X1, X2, X3, X4, X5, X6, X7, X8, X9, X10, X11 represent respectively germinating energy, germination index, coring quantity,
Radicle length, plant height, stem number, stem are thick, the salt tolerant efficiency of vitality index, root Fresh Yuxincao, Root dry weight and root volume；
S6, the salt tolerance of kind judged according to the size of comprehensive resistance to salt figure, D values are bigger, and the salt damage being subject to is smaller, kind it is resistance to
Salt is stronger；D values are smaller, and the salt damage being subject to is bigger, and the salt tolerance of kind is weaker.
2. a kind of cotton seeds selection of salt tolerance method according to claim 1, it is characterised in that the artificial seawater is every
Rise and include 26.726 grams of sodium chloride, 2.26 grams of magnesium chloride, 3.248 grams of magnesium sulfate, 1.153 grams of calcium chloride, sodium acid carbonate 0.198
Gram, 0.721 gram of potassium chloride.
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Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105874967B (en) * | 2016-04-20 | 2018-08-14 | 运城学院 | A kind of method of cotton seeds to the Resistance Identification of salt stress |
| CN105900636A (en) * | 2016-04-20 | 2016-08-31 | 运城学院 | Method for screening salt-tolerance kidney bean variety |
| CN105850274B (en) * | 2016-04-20 | 2018-09-25 | 运城学院 | A method of screening salt tolerant cotton variety |
| CN106069696B (en) * | 2016-07-07 | 2019-05-17 | 中国农业科学院棉花研究所 | Semi-wild cotton is to secondary saline and alkaline seedling stage water planting Resistance Identification method |
| CN106613826A (en) * | 2016-11-09 | 2017-05-10 | 中国农业科学院棉花研究所 | Low-potassium-tolerant cotton seedling nutrient solution and application thereof |
| CN109874665B (en) * | 2019-04-11 | 2022-06-14 | 新疆农业科学院经济作物研究所 | Breeding method of salt-tolerant cotton variety based on rapid embryo seedling establishment |
| CN111247898B (en) * | 2020-01-16 | 2022-01-11 | 邯郸市农业科学院 | Screening method of storage-resistant wheat seeds |
| CN112273222A (en) * | 2020-10-22 | 2021-01-29 | 河北省农林科学院旱作农业研究所 | Cotton variety breeding method |
| CN114532221A (en) * | 2022-03-10 | 2022-05-27 | 河北省农林科学院旱作农业研究所 | Method for breeding salt-tolerant cotton variety by using natural seawater |
| CN116058130A (en) * | 2023-04-06 | 2023-05-05 | 安徽中青检验检测有限公司 | Method and device for detecting seed vitality |
| CN117929248B (en) * | 2024-01-25 | 2024-10-01 | 新疆农业科学院经济作物研究所(新疆维吾尔自治区棉花研究所新疆维吾尔自治区甜菜改良中心) | Method and system for identifying salt-tolerant alkalinity of rape in whole growth period based on saline-alkali soil |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102577852A (en) * | 2012-02-14 | 2012-07-18 | 山东棉花研究中心 | Method for identifying salt tolerance of cotton |
| CN103843491A (en) * | 2014-03-25 | 2014-06-11 | 山西省农业科学院棉花研究所 | Method for quickly detecting salt tolerance of cotton variety |
| CN104521474A (en) * | 2014-12-04 | 2015-04-22 | 中国农业科学院棉花研究所 | Cotton variety salt tolerance screening method |
| CN104604555A (en) * | 2015-02-03 | 2015-05-13 | 中国农业科学院棉花研究所 | Method for screening saline-alkaline resistant/tolerant cotton |
-
2015
- 2015-10-29 CN CN201510731582.5A patent/CN105210861B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102577852A (en) * | 2012-02-14 | 2012-07-18 | 山东棉花研究中心 | Method for identifying salt tolerance of cotton |
| CN103843491A (en) * | 2014-03-25 | 2014-06-11 | 山西省农业科学院棉花研究所 | Method for quickly detecting salt tolerance of cotton variety |
| CN104521474A (en) * | 2014-12-04 | 2015-04-22 | 中国农业科学院棉花研究所 | Cotton variety salt tolerance screening method |
| CN104604555A (en) * | 2015-02-03 | 2015-05-13 | 中国农业科学院棉花研究所 | Method for screening saline-alkaline resistant/tolerant cotton |
Non-Patent Citations (1)
| Title |
|---|
| 棉花萌发期和苗期耐盐性评价及耐盐指标筛选;张国伟等;《应用生态学报》;20110831;第22卷(第8期);第2045-2053页，尤其是第2045右栏最后一段及1.2节、第2051页第3.2节 * |
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