CN105900636A - Method for screening salt-tolerance kidney bean variety - Google Patents
Method for screening salt-tolerance kidney bean variety Download PDFInfo
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- CN105900636A CN105900636A CN201610248381.4A CN201610248381A CN105900636A CN 105900636 A CN105900636 A CN 105900636A CN 201610248381 A CN201610248381 A CN 201610248381A CN 105900636 A CN105900636 A CN 105900636A
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- semen phaseoli
- phaseoli vulgaris
- salt
- plant
- fresh weight
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
Abstract
The invention provides a method for screening a salt-tolerance kidney bean variety. The method includes the steps that full, regular and damage-free seeds are selected from different varieties of kidney bean seeds and subjected to sand-cultivation planting through same planting matrixes respectively; after a period of time, seedlings with coincident growth vigor are selected and transplanted into an aerial fog cultivation pass box containing a 1/2 Hoagland nutrient solution to be cultivated into kidney bean plants; when first compound leaves of the kidney bean plants completely expand, the plants with coincident growth vigor are selected, the whole-plant fresh weight X1 is measured and recorded, the plants are divided into a salt treatment group and a blank control group, the plants in the blank control group are still subjected to aerial fog cultivation through a 1/2 Hoagland nutrient solution, and the plants in the salt treatment group are subjected to aerial fog cultivation through a 1/2 Hoagland nutrient solution in which 100 mmol/L of NaCl is added; after preset time, the whole-plant fresh weight of the kidney bean plants in the salt treatment group and the blank control group is measured, and the kidney bean variety with better salt tolerance can be screened out by analyzing and comparing the whole-plate fresh weight data. The method is not high in cost, easy to operate and convenient to implement and popularize at all places.
Description
Technical field
The present invention relates to a kind of method screening Semen Phaseoli Vulgaris kind, particularly relate to a kind of salt tolerant Semen Phaseoli Vulgaris kind of screening
Method.
Background technology
Semen Phaseoli Vulgaris is the Food Legume that China is main, current domestic usual using Semen Phaseoli Vulgaris as accesary foods, consumption
Amount is little.In recent years due to the growth of export volume, Semen Phaseoli Vulgaris cultivated area is driven to increase, but due to soil salting
The restriction changed, the plantation of Semen Phaseoli Vulgaris is severely impacted.The different interracial salt tolerances of Semen Phaseoli Vulgaris there is also bigger difference
Different, can be as modified utilization saline-alkali soil, the pioneer crop of development varieties in saline-alkali areas economy after salt tolerant selection-breeding.
For a long time, screening or the qualification to Salt And Alkali Tolerance Semen Phaseoli Vulgaris, is all to use salt-soda soil, field, artificial salt pond
Test, or the parameter of some Physiology and biochemistries of indoor measurement, as comparison reference.Existing method is basis
Semen Phaseoli Vulgaris material upgrowth situation in salt-soda soil or salt pond carries out salt damage investigation and carries out judging whether have saline and alkaline
Patience.Although the method is directly at field test, qualification result is relatively direct, but annual Semen Phaseoli Vulgaris is only
There is the specific season of growth, particular batch test can only be carried out the most every year；Additionally to identifying that site requirements is higher,
As salinity to have certain gradient, salt pond or place, salt-soda soil to change, because identifying examination through this kind of
The salt-soda soil tested or salt pond are not available to plant other plant, in case other crops are brought salt damage, take up an area
Area is big, and determination rates is low；And salt damage symptom is individual scope qualitatively, does not has concrete digital quantization,
The result difference that different testing persons observes is relatively big, and the conclusion drawn has difference and instability；Field salt
It is naturalness alkali, and the degree of mixedness of soil and saline and alkaline are difficult to unified, although the salinity in salt pond is
The NaCl being manually sprinkled into, but enter after soil, the factor such as the content of soil, pH value and weather rainfall is equal
The change at any time of salinity level can be caused, between year and parallel between the comparability of result of the test poor.
Prior art also has a kind of water planting Salt Tolerance method, instead of soil with the root system environment of artificial creation
Environment, owing to it has many advantages in Tolerant salt, it provides a homogeneous root system ecology ring especially
Border, thus the plant reaction to salt damage can be gone out with Accurate Determining, so having obtained wide in plant salt tolerance is identified
General application.But easily there is the situation of rotten owing to root system of plant carries out anaerobic respiration in water planting.
Therefore lack that a kind of planting environment is easy to control and plant is difficult to rotten, easy-operating screening salt tolerant Semen Phaseoli Vulgaris product
The method planted.
Summary of the invention
In order to solve the problems referred to above, the present invention uses aerial fog cultivation Semen Phaseoli Vulgaris plant selection-breeding salt tolerant Semen Phaseoli Vulgaris kind.Should
The planting environment of method is easily uniformly controlled, cost is lower, the detection cycle is shorter, the accuracy of salt tolerant detection
Higher, plant root environment is difficult to well rotten and easily operated is easy in each local promotion and implementation.
A kind of method screening salt tolerant Semen Phaseoli Vulgaris kind, comprises the following steps:
Step one: the culture fluid of predetermined salt concentration is filled in container；
Step 2: the Semen Phaseoli Vulgaris seed of different cultivars is immersed in culture fluid；
Step 3: after the sowing scheduled time, take predetermined quantity Semen Phaseoli Vulgaris plant and carry out salt tolerance detection；
Step 4: the relatively testing result of each Semen Phaseoli Vulgaris plant.
A kind of method screening salt tolerant Semen Phaseoli Vulgaris kind, comprises the following steps:
Step S1, picks out undamaged seed full, neat from the Semen Phaseoli Vulgaris seed of different cultivars, respectively
Carrying out sand to cultivate and plant, wherein planting matrix is fine sand and the agricultural land soil 1:2 mixing sieved；
Step S2, after sowing 5 days, chooses the seedling that growing way is consistent, transplants to aerial fog cultivation Turnover Box,
Pouring 1/2Hoagland nutritional solution in each aerial fog cultivation Turnover Box into, described 1/2Hoagland nutritional solution makes
With front, pH value is adjusted to 6.0, uses time switch to control the soniclizer in aerial fog cultivation Turnover Box,
Often work 5～15 minutes, have a rest 3～5 minutes, often spend 20～24 hours and change 1/2Hoagland nutritional solution；
Step S3, by step S2 by time fully deployed for Semen Phaseoli Vulgaris seedling culture to first piece compound leaf, selects growing way
Consistent Semen Phaseoli Vulgaris plant also records Herb fresh weight X1, and the Semen Phaseoli Vulgaris plant selected is divided into salt treatment group and blank
Matched group two groups, blank group still uses step S2 to cultivate, and the transplanting of salt treatment group is planted to another aerosol
In training Turnover Box, pouring NaCl concentration in the aerial fog cultivation week vanning of this salt treatment group is 100mmol/L's
1/2Hoagland nutritional solution, uses time switch to control the every work of soniclizer in aerial fog cultivation Turnover Box
Make 10～20 minutes, have a rest 5～10 minutes, often spend 10～15 hours and change the 1/2Hoagland containing NaCl
Nutritional solution；
Step S4, cultivates 10 days according to the incubation step of step S3 again, right to salt treatment group and blank respectively
Semen Phaseoli Vulgaris Herb fresh weight according to group is measured, and the Semen Phaseoli Vulgaris Herb fresh weight of salt treatment group is designated as X2, blank
The Semen Phaseoli Vulgaris Herb fresh weight of group is designated as X3；
Step S5, calculates each strain Semen Phaseoli Vulgaris Herb fresh weight amplitude of variation Y1, wherein Y1=X2-X1, Y1 value
The biggest, show that the amplitude that the Semen Phaseoli Vulgaris Herb fresh weight after salt treatment increases is the biggest, the Semen Phaseoli Vulgaris kind of corresponding kind
The salt tolerance of son is the best；
Step S6, if Y1 numerical value is identical, the Semen Phaseoli Vulgaris salt treatment group of the most same kind and blank group
Herb fresh weight increment Y2, wherein Y2=X3-X2,
Y2 value be on the occasion of time, when other condition of culture are identical, numerical value is the least, then show that salt treatment is with blank right
Photograph ratio, the amplitude that the Herb fresh weight of this Semen Phaseoli Vulgaris kind declines is the least, illustrates that the salt tolerance of corresponding kind is the best,
When Y2 value is negative value, when other condition of culture are identical, the absolute value of this negative value is the biggest, then show at salt
Reason is compared with blank, and the amplitude that the Herb fresh weight of this Semen Phaseoli Vulgaris kind increases is the biggest, and corresponding kind is described
Salt tolerance is the best,
The salt tolerance of correspondence kind when Y2 value is negative value, than Y2 value on the occasion of time correspondence Variety utilization good.
Further, described aerial fog cultivation Turnover Box includes that porous foam plate and casing, every hole insert 1 strain children
Seedling or plant, this porous foam plate is placed in the casing of Turnover Box.Further, described porous foam plate
For expanded polystyrene cystosepiment.
Further, in step S3, each kind of Semen Phaseoli Vulgaris plant of salt treatment group and blank group is at least
Including 3 strain plant.
Further, the method for screening salt tolerant Semen Phaseoli Vulgaris kind the most according to claim 2, its feature exists
In, in step S3 and S4, the Herb fresh weight to Semen Phaseoli Vulgaris plant measures, need to be after wiping plant surface liquid
Measure again and plant do not damaged.
The present invention screening salt tolerant Semen Phaseoli Vulgaris kind method, pick out from the Semen Phaseoli Vulgaris seed of different cultivars full,
Neat undamaged seed carries out sand by same planting matrix respectively and cultivates and plant, it is ensured that sending out of Semen Phaseoli Vulgaris seed
Bud rate and eliminate the impact of other factors outside kind.Choose, after sowing 5 days, the seedling that growing way is consistent
Transplant to the aerial fog cultivation Turnover Box filling 1/2Hoagland nutritional solution, often spend 20～24 hours and change
1/2Hoagland nutritional solution, until seedling culture becomes Semen Phaseoli Vulgaris plant, selects length when first piece of compound leaf is fully deployed
Semen Phaseoli Vulgaris plant that gesture is consistent also records Herb fresh weight X1, and each kind Semen Phaseoli Vulgaris plant selected all is divided into salt
Process group and blank group two groups, blank group still uses 1/2Hoagland nutritional solution to carry out aerosol cultivation
Training, salt treatment group uses the 1/2Hoagland nutritional solution of the NaCl adding 100mmol/L to carry out aerosol cultivation
Training.After spending 10 days, respectively the Semen Phaseoli Vulgaris Herb fresh weight of salt treatment group and blank group is measured, will
The Semen Phaseoli Vulgaris Herb fresh weight of salt treatment group is designated as X2, and the Semen Phaseoli Vulgaris Herb fresh weight of blank group is designated as X3, finally
Calculate each strain Semen Phaseoli Vulgaris Herb fresh weight amplitude of variation Y1 and the Semen Phaseoli Vulgaris salt treatment group of same kind and blank
The Herb fresh weight increment Y2 of group, the data of analysis Y1 and Y2 carry out contrast can filter out the rue more resistant to salt
Bean kind.For the method is compared to prior art, planting environment is easily uniformly controlled, cost is lower, inspection
The survey cycle is shorter, the accuracy of salt tolerant detection is higher, plant root environment be difficult to well rotten and easily operated just
In in each local promotion and implementation.
Accompanying drawing explanation
Fig. 1 is the method flow diagram that the present invention screens salt tolerant Semen Phaseoli Vulgaris kind.
Detailed description of the invention
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clearly
Chu, it is fully described by.Based on the embodiment in the present invention, those of ordinary skill in the art are not making wound
The every other embodiment obtained under the property made work premise, broadly falls into the scope of protection of the invention.
A kind of method screening salt tolerant Semen Phaseoli Vulgaris kind, specifically includes following steps:
The selection-breeding of seed: pick out undamaged seed full, neat from the Semen Phaseoli Vulgaris seed of different cultivars and divide
Not carrying out sand to cultivate and plant, wherein planting matrix is fine sand and the agricultural land soil 1:2 mixing sieved.From different product
The Semen Phaseoli Vulgaris seed planted picks out undamaged seed full, neat and carries out sand by same planting matrix respectively
Cultivate and plant, it is ensured that the germination percentage of Semen Phaseoli Vulgaris seed and eliminate the impact of other factors outside kind.Plantation
The selection of substrate also goes through substantial amounts of experiment, due to the complexity of soil environment and the multiformity of various places, sends out
The planting matrix that a person of good sense finds to use fine sand and the agricultural land soil 1:2 that sieves to be mixed to form can topotype more carefully
Intend the soil environment under nature planting conditions.Directly use unsifted agricultural land soil by the soil on sampling ground
The limitation of environment is very big, and after sieving, directly employing also can be excellent by sampling limiting to of the soil environment on ground
Select the planting matrix that the agricultural land soil after using fine sand and sieving is mixed to form, the fine sand of different proportion and mistake
Fertility and the breathability of planting matrix are also had a great impact by sieve agricultural land soil.Table 1 is that inventor tests
Soil fertility, breathability and the test statistics of the compatible degree with different regions soil environment between different planting matrixs
Result.
Table 1
The cultivation of seedling: after sowing 5 days (3～7 days Semen Phaseoli Vulgaris seedling can be carried out transplanting in general cultivation,
Preferably 5 days), choose the seedling that growing way is consistent, transplant to aerial fog cultivation Turnover Box, this aerial fog cultivation week
Turn the Turnover Box that case includes filling nutritional solution and the porous foam plate being placed in Turnover Box, it is preferred to use 40 holes
Polystyrene foam plate, every hole inserts 1 strain Semen Phaseoli Vulgaris seedling, and each volume is the aerial fog cultivation Turnover Box of 25L
In pour the 1/2Hoagland nutritional solution of 10L into, pH value is adjusted by this 1/2Hoagland nutritional solution before use
Whole is 6.0.Needing at this to particularly point out, soil ph is divided into 9 grades.PH value is pole less than 4.5
Highly acid, 4.5-5.5 is highly acid, and 5.5-6.0 is acid, and 6.0-6.5 is faintly acid, and 6.5-7.0 is neutral,
7.0-7.5 is alkalescence, and 7.5-8.5 is alkalescence, and 8.5-9.5 is strong basicity, is extremely strong alkalescence more than 9.5.Though
So soil is required not strict by Semen Phaseoli Vulgaris, all can grow, have on the pH value all kinds of soil between 5.5-7
Stronger acid and alkali-resistance ability, but inventor is on the basis of great many of experiments, if finding 1/2Hoagland
The pH value of nutritional solution is adjusted to the neutral or faintly acid scopes such as 6.0-7.0, and test Semen Phaseoli Vulgaris survival rate can carry significantly
High and rotten rate all can be substantially reduced, and concrete Contrast on effect sees following table:
Table 2
PH value is adjusted to 6.0 by currently preferred 1/2Hoagland nutritional solution before use.Use timing simultaneously
Nebulizer service intermittent in on-off control aerial fog cultivation Turnover Box, produces nutritional solution aerosol for plant root
Absorbing, it is preferred to use soniclizer, soniclizer is convenient and swift when using, and noiselessness, shake
The factor impact such as dynamic, soniclizer work 5～15 minutes then have a rest 3～5 minutes alternately, warp
Cross experimental verification work 10 minutes then have a rest 4 minutes be optimal discontinuous operation frequency, be also the present invention
The preferred operating frequency of soniclizer, the soniclizer under different operating frequency is to experimental result
Impact is as shown in table 3.
Table 3
In general, nutritional solution consumes the slowest, the least to the loss of nebulizer, growth of seedling state and seedling
The scheme that the absorbance of nutritional solution is the best is more met the requirement of the present invention, wherein works 10 minutes and have a rest 4
Minute scheme works best.
Often cross the nutritional solution changed in Turnover Box for 20～24 hours, prevent nutritional solution volatilization or caused by absorption
Concentration difference excessive, thus keep nutritional solution fresh enough and concentration enough Semen Phaseoli Vulgariss growth.Turnover Box
The volume of volume and nutritional solution can be selected voluntarily, and this is limited by portion of the present invention, and the present invention preferably uses 25L
Turnover Box in add the nutritional solution of 10L be an example.
The packet transaction of plant: by time fully deployed for Semen Phaseoli Vulgaris seedling culture to first piece compound leaf, select growing way one
The Semen Phaseoli Vulgaris plant that causes also records Herb fresh weight X1, and each kind Semen Phaseoli Vulgaris plant selected all is divided into salt treatment
Group and blank group two groups, wherein each kind of Semen Phaseoli Vulgaris plant of salt treatment group and blank group is at least
Including 3 strain plant, better assure that the individual impact on experimental result of different plants.Compound leaf is by majority
Lobule forms, and is divided into palmately compound leaf, trifoliolate leaf, winglike compound leaf etc..The compound leaf of Semen Phaseoli Vulgaris seedling is multiple at three
Leaf, has 3 lobules raw on the top of common petiole.Blank group still uses above-mentioned " cultivation of seedling "
Step is cultivated, and salt treatment group is transplanted to another aerial fog cultivation Turnover Box, and the aerosol of this salt treatment group is planted
Pour, in the vanning of training week, the 1/2Hoagland nutritional solution that NaCl concentration is 100mmol/L into, use time switch
Control the nebulizer service intermittent in aerial fog cultivation Turnover Box, it is preferred to use soniclizer, ultrasound wave
Nebulizer is convenient and swift when using, and the impact of the factor such as noiselessness, vibrations, owing to the packet transaction of plant walks
Semen Phaseoli Vulgaris plant in Zhou is bigger for age than the strain of the plant in seedling culture step, and therefore Semen Phaseoli Vulgaris plant is to nutritional solution
Absorption efficiency and absorbtivity are bigger, and the nutritional solution aerosol that correspondingly soniclizer produces is more sufficient, this
Time nebulizer often work 10～20 minutes, have a rest 5～10 minutes alternately, and often cross 10～15 hours
(within preferably 12 hours, change one time of nutrition liquid and be easy to time control, can respectively change once every day) changes week
Turn the nutritional solution in case, keep nutritional solution fresh enough and the growth of concentration enough Semen Phaseoli Vulgariss.Inventor is through excessive
The most often work 15 minutes after the test of amount, have a rest 7 minutes.Soniclizer under different operating frequency
Impact on the Semen Phaseoli Vulgaris plant being in trophophase is as shown in table 4.
Table 4
Owing to Semen Phaseoli Vulgaris plant is bigger to absorption efficiency and the absorbtivity of nutritional solution than Seedling Stage, different work frequencies
The impact of plant is also not quite similar by rate, and the nutritional solution aerosol that correspondingly soniclizer produces is accomplished by more
Abundance, and work long hours or to frequently switch on the machine loss also to nebulizer bigger.In general, nutrition
Liquid consumes the slowest, the least to the loss of nebulizer, Reducing sugar and plant to the absorbance of nutritional solution more
Good scheme more meets the requirement of the present invention, and the scheme works having a rest 7 minutes for 15 minutes that wherein works is best.
Wherein, the Semen Phaseoli Vulgaris plant selecting first piece of consistent compound leaf of growing way fully deployed is to control initial strip
Part is consistent, is unlikely to interference test result.For the selection of the concentration of NaCl, inventor is by common
Salinized soil carry out research find NaCl concentration be the 1/2Hoagland nutritional solution of 100mmol/L more
Agree with common salt-soda soil environment.Too high, exceed the salinized soil impact on the growth of Semen Phaseoli Vulgaris, typically
Semen Phaseoli Vulgaris can not survive or shaky in too high salinized soil.Too low the most well screening effect,
Difference between salt treatment group and blank group is inconspicuous.Table 5 is containing of the variable concentrations tested of inventor
The impact on Semen Phaseoli Vulgaris of the 1/2Hoagland nutritional solution of NaCl.Wherein, NaCl concentration is too low, does not has and should have
Salt treatment effect, too high then be well beyond existing salt-tolerant plant salt tolerant holding capacity and without reference to property.
Table 5
Herb fresh weight measure: cultivate again after process 10 days (generally 5～15 days, preferably 10 days, during cultivation
Between the shortest there is no obvious gap, oversize Semen Phaseoli Vulgaris plant strain growth is affected by other factors generation error), the most right
The Semen Phaseoli Vulgaris Herb fresh weight of salt treatment group and blank group is measured, by the Semen Phaseoli Vulgaris Herb fresh weight of salt treatment group
Being designated as X2, the Semen Phaseoli Vulgaris Herb fresh weight of blank group is designated as X3；To Semen Phaseoli Vulgaris Herb fresh weight X1, X2 and X3
Mensuration need to measure again after wiping plant surface liquid and plant not damaged, it is ensured that different Semen Phaseoli Vulgariss
Plant growing way is not affected.
Salt tolerance preliminary comparison: calculate each strain Semen Phaseoli Vulgaris Herb fresh weight amplitude of variation Y1, wherein Y1=X2-X1,
Y1 value is the biggest, shows that the amplitude that the Semen Phaseoli Vulgaris Herb fresh weight after salt treatment increases is the biggest, the rue of corresponding kind
The salt tolerance of bean seed is the best；
Salt tolerance contrasts further: if Y1 numerical value is identical, the Semen Phaseoli Vulgaris salt treatment group of the most same kind and
The Herb fresh weight increment Y2 of blank group, wherein Y2=X3-X2, Y2 value be on the occasion of time, other cultivate
When condition is identical, numerical value is the least, then show salt treatment compared with blank, the Herb of this Semen Phaseoli Vulgaris kind is fresh
The amplitude heavily declined is the least, illustrates that the salt tolerance of corresponding kind is the best, and when Y2 value is negative value, other cultivate bar
When part is identical, the absolute value of this negative value is the biggest, then show salt treatment compared with blank, this Semen Phaseoli Vulgaris kind
The amplitude that increases of Herb fresh weight the biggest, illustrate that the salt tolerance of corresponding kind is the best, correspondence when Y2 value is negative value
The salt tolerance of kind, than Y2 value on the occasion of time correspondence Variety utilization good.
Additionally, due to the legume growth characteristics such as Semen Phaseoli Vulgaris, Semen arachidis hypogaeae are similar, therefore use above-mentioned identical or phase
As parameter detect, the best salt tolerance Detection results can be obtained.
The above, the only present invention preferably detailed description of the invention, but protection scope of the present invention not office
Being limited to this, any those familiar with the art is in the technical scope that the invention discloses, according to this
The technical scheme of invention and inventive concept thereof in addition equivalent or change, all should contain the protection in the present invention
Within the scope of.
Claims (6)
1. the method screening salt tolerant Semen Phaseoli Vulgaris kind, it is characterised in that comprise the steps:
Step one: the culture fluid of predetermined salt concentration is filled in container；
Step 2: the Semen Phaseoli Vulgaris seed of different cultivars is immersed in culture fluid；
Step 3: after the sowing scheduled time, take predetermined quantity Semen Phaseoli Vulgaris plant and carry out salt tolerance detection；
Step 4: the relatively testing result of each Semen Phaseoli Vulgaris plant.
2. the method screening salt tolerant Semen Phaseoli Vulgaris kind, it is characterised in that comprise the following steps:
Step S1, picks out undamaged seed full, neat from the Semen Phaseoli Vulgaris seed of different cultivars, respectively
Carrying out sand to cultivate and plant, wherein planting matrix is fine sand and the agricultural land soil 1:2 mixing sieved；
Step S2, after sowing 5 days, chooses the seedling that growing way is consistent, transplants to aerial fog cultivation Turnover Box,
Pouring 1/2Hoagland nutritional solution in each aerial fog cultivation Turnover Box into, described 1/2Hoagland nutritional solution makes
With front, pH value is adjusted to 6.0, uses time switch to control the soniclizer in aerial fog cultivation Turnover Box,
Often work 5～15 minutes, have a rest 3～5 minutes, often spend 20～24 hours and change 1/2Hoagland nutritional solution；
Step S3, by step S2 by time fully deployed for Semen Phaseoli Vulgaris seedling culture to first piece compound leaf, selects growing way
Consistent Semen Phaseoli Vulgaris plant also records Herb fresh weight X1, and the Semen Phaseoli Vulgaris plant selected is divided into salt treatment group and blank
Matched group two groups, blank group still uses step S2 to cultivate, and the transplanting of salt treatment group is planted to another aerosol
In training Turnover Box, pouring NaCl concentration in the aerial fog cultivation week vanning of this salt treatment group is 100mmol/L's
1/2Hoagland nutritional solution, uses time switch to control the every work of soniclizer in aerial fog cultivation Turnover Box
Make 10～20 minutes, have a rest 5～10 minutes, often spend 10～15 hours and change the 1/2Hoagland containing NaCl
Nutritional solution；
Step S4, cultivates 10 days according to the incubation step of step S3 again, right to salt treatment group and blank respectively
Semen Phaseoli Vulgaris Herb fresh weight according to group is measured, and the Semen Phaseoli Vulgaris Herb fresh weight of salt treatment group is designated as X2, blank
The Semen Phaseoli Vulgaris Herb fresh weight of group is designated as X3；
Step S5, calculates each strain Semen Phaseoli Vulgaris Herb fresh weight amplitude of variation Y1, wherein Y1=X2-X1, Y1 value
The biggest, show that the amplitude that the Semen Phaseoli Vulgaris Herb fresh weight after salt treatment increases is the biggest, the Semen Phaseoli Vulgaris kind of corresponding kind
The salt tolerance of son is the best；
Step S6, if Y1 numerical value is identical, the Semen Phaseoli Vulgaris salt treatment group of the most same kind and blank group
Herb fresh weight increment Y2, wherein Y2=X3-X2,
Y2 value be on the occasion of time, when other condition of culture are identical, numerical value is the least, then show that salt treatment is with blank right
Photograph ratio, the amplitude that the Herb fresh weight of this Semen Phaseoli Vulgaris kind declines is the least, illustrates that the salt tolerance of corresponding kind is the best,
When Y2 value is negative value, when other condition of culture are identical, the absolute value of this negative value is the biggest, then show at salt
Reason is compared with blank, and the amplitude that the Herb fresh weight of this Semen Phaseoli Vulgaris kind increases is the biggest, and corresponding kind is described
Salt tolerance is the best,
The salt tolerance of correspondence kind when Y2 value is negative value, than Y2 value on the occasion of time correspondence Variety utilization good.
The method of screening salt tolerant Semen Phaseoli Vulgaris kind the most according to claim 2, it is characterised in that described gas
Mist cultivation Turnover Box includes that porous foam plate and casing, every hole insert 1 strain seedling or plant, this porous foam
Plate is placed in the casing of Turnover Box.
The method of screening salt tolerant Semen Phaseoli Vulgaris kind the most according to claim 3, it is characterised in that described many
Hole cystosepiment is expanded polystyrene cystosepiment.
The method of screening salt tolerant Semen Phaseoli Vulgaris kind the most according to claim 2, it is characterised in that step S3
The each kind of Semen Phaseoli Vulgaris plant of middle salt treatment group and blank group at least includes 3 strain plant.
The method of screening salt tolerant Semen Phaseoli Vulgaris kind the most according to claim 2, it is characterised in that step S3
With in S4, the Herb fresh weight of Semen Phaseoli Vulgaris plant is measured, need to measure again and the most right after wiping plant surface liquid
Plant damages.
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| CN113229076A (en) * | 2021-06-04 | 2021-08-10 | 沧州市农林科学院 | Wheat breeding method for screening excellent salt-tolerant strains by using salt pond |
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