CN105875403B - A method of screening salt tolerant sorghum variety - Google Patents

Abstract

The present invention provides a kind of methods of screening salt tolerant sorghum variety, the method is picked out full from the sorghum seeds of different cultivars, neat undamaged seed carries out sand culture plantation with same planting matrix respectively, it chooses in the consistent seedling replanting to the aerial fog cultivation turnover box for fill 1/2Hoagland nutrient solutions of growing way and is cultivated into sorghum plant after sowing a period of time, it selects 2 leaf, 1 heart and the consistent plant of growing way records complete stool leaf area X1 and is divided into two groups of salt treatment group and blank control group, blank control group still uses 1/2Hoagland nutrient solutions to carry out aerial fog cultivation, salt treatment group carries out aerial fog cultivation using the 1/2Hoagland nutrient solutions for the NaCl for adding 100mmol/L.It crosses after the predetermined time, the sorghum complete stool leaf area of salt treatment group and blank control group is measured respectively, the sorghum variety more resistant to salt can be filtered out by the analysis comparison of complete stool blade face volume data.This method cost is not high, easily operated convenient in each local promotion and implementation.

Description

A method of screening salt tolerant sorghum variety

Technical field

The present invention relates to a kind of method of screening sorghum variety more particularly to a kind of methods of screening salt tolerant sorghum variety.

Background technology

Sorghum is the grain and forage crop that cultivation history is longer in the world, and output is high, have drought resisting, saline-alkali tolerant, The features such as waterlogging, impoverishment tolerant.But there are larger differences for salt tolerance between sorghum different cultivars.The sorghum grain important as China is made Object occupies very important status in the national economic development.Due to the restriction of the soil salinization, the plantation of sorghum is by serious It influences.Sorghum itself has saline-alkali tolerant characteristic, by can be used as modified utilization saline-alkali soil, development varieties in saline-alkali areas after salt tolerant selection and breeding Economic pioneer crop.

For a long time, to the screening or identification of saline-alkali tolerant sorghum, tried using field salt-soda soil, artificial salt pond It tests or the parameter of indoor measurement some Physiology and biochemistries, as comparison reference.Existing method be according to sorghum material in salt-soda soil or Upgrowth situation progress salt damage investigation is judged whether there is patience to saline and alkaline in person salt pond.Although this method is directly in field Identification, qualification result is relatively direct, but annual sorghum only has the specific season of growth, i.e., can only carry out particular batch every year Experiment；In addition higher to identification site requirements, if salinity will have certain gradient, salt pond or salt-soda soil place that can not replace, Because the salt-soda soil or salt pond by this kind of qualification test are not available to plantation other plant, to prevent bringing salt to other crops Evil, takes up a large area, determination rates are low；And salt damage symptom is a qualitative range, it is different without specific digital quantization The result difference of testing person's observation is larger, and the conclusion obtained has difference and unstable；Field salt-soda soil is nature, And the degree of mixedness and saline and alkaline of soil are not easy unification, although the salinity in salt pond is the NaCl being manually sprinkled into, but after entrance soil, The factors such as the content of soil, pH value and weather rainfall can cause the variation at any time of salinity level, be tried between year and between parallel The comparativity for testing result is poor.

The prior art also has a kind of water planting Salt Tolerance method, with the root system environment of artificial creation instead of soil environment, Since it is in many of Tolerant salt advantage, especially it provides a uniform root system ecological environment, thus can be accurate Reaction of the plant to salt damage is determined, so being widely applied in plant salt tolerance identification.But water planting is due to plant roots System carries out the case where anaerobic respiration is susceptible to rotten.

Therefore lack that a kind of planting environment is easy to control and plant is not easy rotten, the side of easy-operating screening salt tolerant sorghum variety Method.

Invention content

To solve the above-mentioned problems, the present invention uses aerial fog cultivation sorghum plant selection and breeding salt tolerant sorghum variety.This method Planting environment is easy to be uniformly controlled, plant root environment is not easy well rotten, and easily operated is convenient for promotion and implementation.

A method of screening salt tolerant sorghum variety includes the following steps：

Step 1：The culture solution of predetermined salinity is filled into container；

Step 2：The sorghum seeds of different cultivars are immersed in culture solution；

Step 3：After sowing the predetermined time, predetermined quantity sorghum plant is taken to carry out salt tolerance detection；

Step 4：Compare the testing result of each sorghum plant.

A method of screening salt tolerant sorghum variety includes the following steps：

Step S1 picks out full, neat undamaged seed from the sorghum seeds of different cultivars, carries out sand culture respectively Plantation, wherein planting matrix are the agricultural land soil 2 of fine sand and sieving:1 mixing；

Step S2 after sowing 5 days, chooses the consistent seedling of growing way, and in transplanting to aerial fog cultivation turnover box, each aerosol is planted 1/2Hoagland nutrient solutions are poured into training turnover box, pH value is being adjusted to 7.0 by the 1/2Hoagland nutrient solutions using preceding, Ultrasonic ultrasonic delay line memory in aerial fog cultivation turnover box is controlled using time switch, is often worked 5~15 minutes, is rested 3~5 minutes, It often spends 20~24 hours and replaces 1/2Hoagland nutrient solutions；

Step S3 when cultivating sorghum seedling to 21 heart of leaf by step S2, selects consistent 2 leaf, 1 heart sorghum plant of growing way And complete stool leaf area X1 is recorded, the sorghum plant selected is divided into two groups of salt treatment group and blank control group, blank control group It is still cultivated using step S2, salt treatment group is transplanted into another aerial fog cultivation turnover box, the aerial fog cultivation of the salt treatment group The 1/2Hoagland nutrient solutions that NaCl concentration is 100mmol/L are poured into week vanning, using time switch control aerial fog cultivation week Ultrasonic ultrasonic delay line memory in turnning box often works 10~20 minutes, rests 5~10 minutes, often spends 10~15 hours and replaces and contains NaCl 1/2Hoagland nutrient solutions；

Step S4 is cultivated 20 days according to the incubation step of step S3, again respectively to the height of salt treatment group and blank control group Fine strain of millet complete stool leaf area is measured, and the sorghum complete stool leaf area of salt treatment group is denoted as X2, the sorghum complete stool leaf of blank control group Area is denoted as X3；

Step S5 calculates each plant height fine strain of millet complete stool leaf area amplitude of variation Y1, and wherein Y1=X2-X1, Y1 values are bigger, show The increased amplitude of sorghum complete stool leaf area after salt treatment is bigger, and the salt tolerance of the sorghum seeds of corresponding kind is better；

Step S6, if Y1 numerical value is identical, the sorghum salt treatment group of more same kind and the complete stool leaf of blank control group Area increase Y2, wherein Y2=X3-X2,

When Y2 values are positive value, when other condition of culture are identical, numerical value is smaller, then shows salt treatment compared with blank control, The amplitude that the complete stool leaf area of the sorghum variety declines is smaller, illustrates that the salt tolerance of corresponding kind is better,

When Y2 values are negative value, when other condition of culture are identical, the absolute value of the negative value is bigger, then shows the same blank of salt treatment Control is compared, and the increased amplitude of complete stool leaf area of the sorghum variety is bigger, illustrates that the salt tolerance of corresponding kind is better,

Y2 values correspond to the salt tolerance of kind when being negative value, got well when corresponding to Variety utilization when Y2 values are positive value.

Further, the aerial fog cultivation turnover box in step S2 and/or S3 includes porous foam plate and babinet, is inserted into per hole 1 plant of seedling or plant, the porous foam plate are placed in the babinet of turnover box.Further, the porous foam plate is porous Polystyrene foam plate.

Further, each kind of the sorghum plant of salt treatment group and blank control group includes at least 3 plants in step S3 Plant.

The method of the screening salt tolerant sorghum variety of the present invention, is picked out full, neat from the sorghum seeds of different cultivars Undamaged seed carries out sand culture plantation with same planting matrix respectively, ensure that the germination percentage of sorghum seeds and eliminates product The influence of other factors except kind.Sowing chooses the consistent seedling replanting of growing way to filling 1/2Hoagland nutrition after 5 days In the aerial fog cultivation turnover box of liquid, often spends 20~24 hours and replace 1/2Hoagland nutrient solutions until seedling culture is planted at sorghum Strain, each kind sorghum plant for selecting consistent 2 leaf, 1 heart sorghum plant of growing way and recording complete stool leaf area X1, and selecting All it is divided into two groups of salt treatment group and blank control group, blank control group still uses 1/2Hoagland nutrient solutions to carry out aerial fog cultivation, Salt treatment group carries out aerial fog cultivation using the 1/2Hoagland nutrient solutions for the NaCl for adding 100mmol/L.It crosses after 20 days, The sorghum complete stool leaf area of salt treatment group and blank control group is measured respectively, by the sorghum complete stool leaf area of salt treatment group It is denoted as X2, the sorghum complete stool leaf area of blank control group is denoted as X3, finally calculates each plant height fine strain of millet complete stool leaf area amplitude of variation The data of the complete stool leaf area increment Y2 of the sorghum salt treatment group and blank control group of Y1 and same kind, analysis Y1 and Y2 carry out Comparison can filter out the sorghum variety more resistant to salt.This method compared with the prior art for, cost is lower, detection cycle more Short, salt tolerant detection accuracy higher, and it is easily operated, convenient in each local promotion and implementation.

Description of the drawings

Fig. 1 is the method flow diagram of present invention screening salt tolerant sorghum variety.

Specific implementation mode

Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Site preparation describes.Based on the embodiments of the present invention, those of ordinary skill in the art institute without creative efforts The every other embodiment obtained, shall fall within the protection scope of the present invention.

A method of screening salt tolerant sorghum variety specifically includes following steps：

The selection and breeding of seed：Full, neat undamaged seed is picked out from the sorghum seeds of different cultivars to carry out respectively Sand culture is planted, and wherein planting matrix is the agricultural land soil 2 of fine sand and sieving:1 mixing.It is selected from the sorghum seeds of different cultivars Go out full, neat undamaged seed and carry out sand culture plantation with same planting matrix respectively, ensure that the germination of sorghum seeds Rate and the influence for eliminating the other factors except kind.The selection of planting matrix also goes through a large amount of experiment, due to various regions Soil environment complexity and diversity, inventor find using fine sand and sieving agricultural land soil 2：1 kind being mixed to form Phytyl matter can more carefully under simulation nature planting conditions soil environment.Directly taken using unsifted agricultural land soil The limitation of the soil environment on sample ground is very big, and directly being used after sieving can also be limited to by the soil environment on sampling ground, excellent The planting matrix that choosing is mixed to form using the agricultural land soil after fine sand and sieving, the fine sand and sieving agricultural land soil of different proportion Fertility and gas permeability to planting matrix also have a great impact.Table 1 is soil fertilizer between the different planting matrixs that inventor tests Power, gas permeability and the test statistics result with the compatible degree of different regions soil environment.

Table 1

The cultivation of seedling：Sowing (generally cultivates 3~7 days sorghum seedlings to can be carried out transplanting, preferably 5 days) after 5 days, The consistent seedling of growing way is chosen, in transplanting to aerial fog cultivation turnover box, which includes the week for filling nutrient solution Turnning box and the porous foam plate being placed in turnover box, it is preferred to use the polystyrene foam plate in 40 holes is inserted into 1 plant height fine strain of millet children per hole Seedling, to pour into the 1/2Hoagland nutrient solutions of 10L in the aerial fog cultivation turnover box of 25L, which seeks each volume PH value is being adjusted to 7.0 by nutrient solution using preceding.It needs to particularly point out herein, soil ph is divided into 9 grades.PH value is less than 4.5 be extremely strong acidity, and 4.5-5.5 is highly acid, and 5.5-6.0 is acidity, and 6.0-6.5 is faintly acid, and 6.5-7.0 is neutrality, 7.0-7.5 is alkalescent, and 7.5-8.5 is alkalinity, and 8.5-9.5 is strong basicity, and it is extremely strong alkalinity to be more than 9.5.Although sorghum is to soil Earth requirement is not stringent, can be grown on all kinds of soil between 5-8.5 in pH value, have stronger acidproof alkali ability, still Inventor on the basis of many experiments, if find by the pH value of 1/2Hoagland nutrient solutions be adjusted to 6.5-7.5 etc. it is neutral or Alkalescent range, experiment sorghum survival rate can greatly improve and rotten rate can all substantially reduce, and specific Contrast on effect is referring to following table：

Table 2

PH value is being adjusted to 7.0 by currently preferred 1/2Hoagland nutrient solutions using preceding.Time switch is used simultaneously The atomizer service intermittent in aerial fog cultivation turnover box is controlled, nutrient solution aerosol is generated and is absorbed for plant root, it is preferred to use Ultrasonic ultrasonic delay line memory, the factors such as convenient and efficient and noiseless when using, vibrations of ultrasonic ultrasonic delay line memory influence, ultrasonic ultrasonic delay line memory It works 5~15 minutes and then rests 3~5 minutes alternately, work 10 minutes by experimental verification and then rest 4 minutes as most Good discontinuous operation frequency, and the ultrasonic ultrasonic delay line memory of the present invention preferred working frequency, the ultrasound under different operating frequency Influence of the wave atomizer to experimental result is as shown in table 3.

Table 3

In general, nutrient solution consumes slower, smaller to the loss of atomizer, growth of seedling state and seedling to nutrient solution The better scheme of absorptivity more meet the requirement of the present invention, wherein the scheme works that 10 minutes rest 4 minutes that work are best.

20~24 hours nutrient solutions replaced in turnover box are often crossed, nutrient solution volatilization or the concentration caused by absorbing are prevented Difference is excessive, and to keep, nutrient solution is fresh enough and the enough sorghum growths of concentration.The volume of turnover box and the body of nutrient solution Product can be selected voluntarily, and portion of the present invention limits this, and the present invention is preferably with the nutrient solution that 10L is added in the turnover box of 25L It is an example.

The packet transaction of plant：When sorghum seedling is cultivated to 21 heart of leaf, consistent 2 leaf, 1 heart sorghum plant of growing way is selected And complete stool leaf area X1 is recorded, and each kind sorghum plant selected all is divided into two groups of salt treatment group and blank control group, Wherein each kind of the sorghum plant of salt treatment group and blank control group includes at least 3 plants of plant, better assures that difference Influence of the individual plants to experimental result.2 leaf of sorghum seedling, 1 heart refers to a piece of not yet with two panels fully expanded leaves and center The state of leaf is unfolded.Blank control group is still cultivated using above-mentioned " cultivation of seedling " step, and salt treatment group is transplanted to another In aerial fog cultivation turnover box, 1/ that NaCl concentration is 100mmol/L is poured into the aerial fog cultivation week vanning of the salt treatment group 2Hoagland nutrient solutions control the atomizer service intermittent in aerial fog cultivation turnover box, it is preferred to use super using time switch Sonic nebulizers, the factors such as convenient and efficient and noiseless when using, vibrations of ultrasonic ultrasonic delay line memory influence, due to the grouping of plant Sorghum plant in processing step is bigger than the strain age of the plant in seedling culture step, therefore absorption of the sorghum plant to nutrient solution Efficiency and uptake bigger, the nutrient solution aerosol that correspondingly ultrasonic ultrasonic delay line memory generates is more sufficient, and atomizer at this time is per work Make 10~20 minutes, rest 5~10 minutes alternately, and often spends 10~15 hours and (replace one time of nutrition liquid within preferably 12 hours Convenient for time control, sooner or later each daily can replace primary) replace nutrient solution in turnover box, keep nutrient solution fresh enough and The enough sorghum growths of concentration.Inventor preferably often works 15 minutes after a large amount of experiment, rests 7 minutes.Different operating Influence of the ultrasonic ultrasonic delay line memory to the sorghum plant in growth period is as shown in table 4 under frequency.

Table 4

Due to sorghum plant than Seedling Stage to the absorption efficiency and uptake bigger of nutrient solution, different working frequencies is to planting The influence of strain is also not quite similar, and correspondingly the nutrient solution aerosol of ultrasonic ultrasonic delay line memory generation just needs more sufficient, and long-time work Make or to frequently switch on machine also larger to the loss of atomizer.In general, nutrient solution consumption it is slower, to the loss of atomizer more The better scheme of the absorptivity of small, Reducing sugar and plant pair nutrient solution more meets the requirement of the present invention, wherein working 15 The scheme works that minute rests 7 minutes are best.

Wherein, it is consistent in order to control primary condition to select consistent 2 leaf, 1 heart sorghum plant of growing way, is unlikely to interference examination Test result.Selection for the concentration of NaCl, inventor have found NaCl concentration by carrying out research to common salinized soil More agree with common salt-soda soil environment for the 1/2Hoagland nutrient solutions of 100mmol/L.It is excessively high, it has been more than salinized soil Influence to the growth of sorghum, general sorghum cannot survive or shaky in excessively high salinized soil.It is too low, do not have Good screening effect, the difference unobvious between salt treatment group and blank control group.Table 5 is the various concentration of inventor's experiment Influence of the 1/2Hoagland nutrient solutions to sorghum containing NaCl.Wherein, NaCl concentration is too low, does not have due salt treatment Effect, the excessively high salt tolerant for being then well beyond existing salt-tolerant plant are withstood forces without referential.

Table 5

Complete stool Leaf area determination：Cultivating 20 days after processing again, (generally 15~25 days, preferably 20 days, the cultivation time was too short There is no apparent gap, too long Sorghum Leaves growth, which is affected by other factors, generates error), respectively to salt treatment group and blank control The sorghum complete stool leaf area of group is measured, and the sorghum complete stool leaf area of salt treatment group is denoted as X2, the sorghum of blank control group Complete stool leaf area is denoted as X3；The measurement of sorghum complete stool leaf area X1, X2 and X3 is preferably used live body blade face analyzer from 3 not It averages after same angle measurement sorghum complete stool leaf area.Live body blade face analyzer is existing obtainable Leaf area determination instrument Device, the blade that can be not only directed to any irregular shape, any color, any thickness and water content carry out area measurement. The area of individual blade can also be surveyed or multiple blades while being measured, show the shape and area data of blade, and energy respectively Measure and count the pityriasis simplex size on blade.

Salt tolerance preliminary comparison：Calculate each plant height fine strain of millet complete stool leaf area amplitude of variation Y1, wherein Y1=X2-X1, Y1 values It is bigger, show that the increased amplitude of sorghum complete stool leaf area after salt treatment is bigger, the salt tolerant of the sorghum seeds of corresponding kind Property is better；

Salt tolerance further compares：If Y1 numerical value is identical, the sorghum salt treatment group of more same kind and blank control The complete stool leaf area increment Y2 of group, wherein Y2=X3-X2, when Y2 values are positive value, when other condition of culture are identical, numerical value is smaller, Then show salt treatment compared with blank control, the amplitude that the complete stool leaf area of the sorghum variety declines is smaller, illustrates corresponding kind Salt tolerance it is better, Y2 values be negative value when, when other condition of culture are identical, the absolute value of the negative value is bigger, then shows salt treatment Compared with blank control, the increased amplitude of complete stool leaf area of the sorghum variety is bigger, illustrates that the salt tolerance of corresponding kind is better, Y2 values correspond to the salt tolerance of kind when being negative value, got well when corresponding to Variety utilization when Y2 values are positive value.

Further, since the stalk types gramineous crop growth characteristics such as sorghum, sugarcane are similar, therefore use above-mentioned identical or phase As parameter be detected, same good salt tolerance detection result can be obtained.In addition, sunflower growth characteristics connect with sorghum It can select to be close when closely, using ultrasonic wave aerosol culture or identical running parameter, soil parameters can also keep identical Or it is similar, actually detected effect is still good.

The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto, Any one skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.

Claims (1)

1. a kind of method of screening salt tolerant sorghum variety, which is characterized in that include the following steps：

Step S1 picks out full, neat undamaged seed from the sorghum seeds of different cultivars, carries out sand culture kind respectively It plants, wherein planting matrix is the agricultural land soil 2 of fine sand and sieving:1 mixing；

Step S2 after sowing 5 days, chooses the consistent seedling of growing way, in transplanting to aerial fog cultivation turnover box, each aerial fog cultivation week 1/2Hoagland nutrient solutions are poured into turnning box, pH value is being adjusted to 7.0 using preceding, used by the 1/2Hoagland nutrient solutions Time switch controls the ultrasonic ultrasonic delay line memory in aerial fog cultivation turnover box, often works 5~15 minutes, rests 3~5 minutes, often mistake Replace 1/2Hoagland nutrient solutions within 20~24 hours；The aerial fog cultivation turnover box includes porous foam plate and babinet, is inserted per hole Enter 1 plant of seedling or plant, which is placed in the babinet of turnover box, wherein the porous foam plate is porous polyphenyl Vinyl foam plate；

Step S3 when cultivating sorghum seedling to 21 heart of leaf by step S2, selects consistent 2 leaf, 1 heart sorghum plant of growing way and remembers The lower complete stool leaf area X1 of record, is divided into two groups of salt treatment group and blank control group, blank control group is still adopted by the sorghum plant selected It is cultivated with step S2, salt treatment group is transplanted into another aerial fog cultivation turnover box, the aerial fog cultivation week dress of the salt treatment group The 1/2Hoagland nutrient solutions that NaCl concentration is 100mmol/L are poured into case, and aerial fog cultivation turnover box is controlled using time switch In ultrasonic ultrasonic delay line memory often work 10~20 minutes, rest 5~10 minutes, often cross 10~15 hours replace containing NaCl 1/ 2Hoagland nutrient solutions；Wherein, each kind of the sorghum plant of salt treatment group and blank control group includes at least 3 plants of plant；

Step S4 is cultivated 20 days again according to the incubation step of step S3, complete to the sorghum of salt treatment group and blank control group respectively Strain leaf area is measured, and the sorghum complete stool leaf area of salt treatment group is denoted as X2, the sorghum complete stool leaf area of blank control group It is denoted as X3；

Step S5 calculates each plant height fine strain of millet complete stool leaf area amplitude of variation Y1, and wherein Y1=X2-X1, Y1 values are bigger, show by The increased amplitude of sorghum complete stool leaf area after salt treatment is bigger, and the salt tolerance of the sorghum seeds of corresponding kind is better；

Step S6, if Y1 numerical value is identical, the sorghum salt treatment group of more same kind and the complete stool leaf area of blank control group Increment Y2, wherein Y2=X3-X2,

When Y2 values are positive value, when other condition of culture are identical, numerical value is smaller, then shows salt treatment compared with blank control, the height The amplitude that the complete stool leaf area of fine strain of millet kind declines is smaller, illustrates that the salt tolerance of corresponding kind is better,

When Y2 values are negative value, when other condition of culture are identical, the absolute value of the negative value is bigger, then shows the same blank control of salt treatment It compares, the increased amplitude of complete stool leaf area of the sorghum variety is bigger, illustrates that the salt tolerance of corresponding kind is better,

Y2 values correspond to the salt tolerance of kind when being negative value, got well when corresponding to Variety utilization when Y2 values are positive value.