CN104003799B - A kind of Nutrition drought resistant agent for plant - Google Patents
A kind of Nutrition drought resistant agent for plant Download PDFInfo
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- CN104003799B CN104003799B CN201410225514.7A CN201410225514A CN104003799B CN 104003799 B CN104003799 B CN 104003799B CN 201410225514 A CN201410225514 A CN 201410225514A CN 104003799 B CN104003799 B CN 104003799B
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Abstract
The present invention relates to a kind of Nutrition drought resistant agent for plant strengthening crop alimentary, improve crop drought resistance ability, comprise by the standby solution of the mixed raw material of following concentration expressed in percentage by weight component: Whitfield's ointment 13.81ppm ~ 82.8ppm, forulic acid 8.0ppm ~ 22.0ppm, vanillic acid 8.0ppm ~ 20ppm, Quercetin 7.0 ~ 12.0ppm, luteolin 7.0 ~ 12.0ppm, potassium silicate 46.2 ~ 184.8ppm, potassium primary phosphate 0.5 ~ 1.0%, urea 1 ~ 1.5%, zinc sulfate 0.25 ~ 0.55%, surplus is water.Utilize the present invention to promote plant growth, while improving resistance, increase Antioxidants in wheat grain, improve its nutritional quality.
Description
Technical field
The invention belongs to plant-growth regulator field, be specifically related to a kind of Nutrition drought resistant agent for plant strengthening crop alimentary, improve crop drought resistance ability.
Background technology
China's water resources is seriously deficient, is only equivalent to world occupancy volume per person's 1/4, belongs to one of 13 poor-water states in the world.Along with increasing of expanding economy and the size of population, to the year two thousand fifty, China is by lack of water 1,000 hundred million m
3, the situation of China's Proposals of Water Resources will be more severe.Drought and water shortage becomes the main limiting factor of China's grain-production, and water deficit is ubiquitous problem in agriculture production, and by the impact of physical environment, majority of plants undergos the threat of scholar's earth water stress all to some extent.Wheat is China's staple food crop, and in the face of the present situation that growing population pressure and water resources are day by day deficient, study on drought resistance is the important research content in agriculture production always.
Phenols is interior important secondary metabolite in plant.Aldehydes matter in wheat mainly comprises phenolic acid, vanillic acid and some flavonoid substances.Phenolic acid and flavonoids are the important non-enzymatic antioxidants of a class, play a significant role in the degeneration-resistant process of plant.Research shows under drought condition, and in plant body, always the substances content such as phenol, total flavonoid increases, and plays an important role to plant opposing drought stress.Meanwhile, the flavonoid compound in plant materials has coerces provide protection, can hinder the abiotic stress such as UV, heavy metal, high temperature to the injury of plant materials.Meanwhile, the material such as phenols has obvious effect to plant disease-resistant, germ resistance.Whitfield's ointment is also the simple aldehydes matter of one produced in plant materials, has multiple effect in plant materials, can growth promoting effects, improves resistance etc.Therefore, utilize this material to carry out soaking seed, Gen Shi or foliage-spray and other methods of application to improve the drought resistance of plant, alleviate the injury of arid to plant, improve the output of crop under adverse circumstance, a new approach can be provided for crop drought resistance.
Also there are some Technology of Drought-resistant to comprise drought resisting preparation at present, more commonly adopt water-holding agent prepared by polymer water-absorbent, as CN98114512.4, but can only crop emerges be used for.Have the preparation adopting the anti-transpiration in blade face, as CN201110390028.7, this anti-rising preparation, by pore, suppress rising, but drought resistance is unstable, may be suppressed leaf photosynthesis simultaneously.Also some foliage fertilizers are had to improve resistance by strengthening plant nutrition, but mainly at later stage foliage-spray.Although CN201110440703 can spray in seedling stage, also can at later stage foliage-spray, its drought resisting is mainly by osmotic adjustment proline(Pro), and limited use in the reaction that proline(Pro) is coerced at Wheat Drought.At present, about the patent of concrete phenolic acid in Drought-resistance in Wheat does not also have, Chinese patent CN101982047B report utilizes external source phenolic acid to coerce to cultivate the stem and leaf of Wheat being rich in antioxidant component.Therefore, configure and a kind ofly there is nutrition and have the preparation of drought resistance function, not only can promote vine growth and development, there is alleviation drought simultaneously, promote crop yield, be also improved the effect of Kernel nutritional character simultaneously.
Summary of the invention
The invention reside in and provide a kind of drought resisting effective, and crop alimentary situation can be improved, promote the Nutrition drought resistant agent for plant of yield and quality.The drought resisting nutrition agent that the present invention utilizes the compounding containing phenolic acid etc. to become, according to crop Different growth phases, adopts the mode soaked or spray respectively, promotes crop growth, increase Antioxidants in crop, improve crop drought resistance.
For realizing above object, the technical solution used in the present invention is:
The invention provides a kind of Nutrition drought resistant agent for plant, comprise by the standby solution of the mixed raw material of following concentration expressed in percentage by weight component: Whitfield's ointment 13.81ppm ~ 82.8ppm, forulic acid 8.0ppm ~ 22.0ppm, vanillic acid 8.0ppm ~ 20ppm, Quercetin 7.0 ~ 12.0ppm, luteolin 7.0 ~ 12.0ppm, potassium silicate 46.2 ~ 184.8ppm, potassium primary phosphate 0.5 ~ 1.0%, urea 1 ~ 1.5%, zinc sulfate 0.25 ~ 0.55%, surplus is water.
According to above-mentioned Nutrition drought resistant agent for plant, the solution be made up of following weight percent concentration component: Whitfield's ointment 20.7ppm ~ 75.9ppm, forulic acid 12.0ppm ~ 19.0ppm, vanillic acid 12.0ppm ~ 16.8ppm, Quercetin 8 ~ 10.0ppm, luteolin 8 ~ 10.0ppm, potassium silicate 77 ~ 154ppm, potassium primary phosphate 0.5 ~ 0.8%, urea 1 ~ 1.2%, zinc sulfate 0.25 ~ 0.55%, surplus is water.
According to above-mentioned Nutrition drought resistant agent for plant, the solution be made up of following weight percent concentration component: Whitfield's ointment 34.8ppm, forulic acid 19.0ppm, vanillic acid 16.8ppm, Quercetin 10.0ppm, luteolin 10.0ppm, potassium silicate 77ppm, potassium primary phosphate 0.5%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
According to above-mentioned Nutrition drought resistant agent for plant, the solution be made up of following weight percent concentration component: Whitfield's ointment 55.2ppm, forulic acid 19.0ppm, vanillic acid 16.8ppm, Quercetin 10.0ppm, luteolin 10.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.1%, zinc sulfate 0.55%, surplus is water.
According to above-mentioned Nutrition drought resistant agent for plant, the solution be made up of following weight percent concentration component: Whitfield's ointment 20.7ppm, forulic acid 19.0ppm, vanillic acid 16.8ppm, Quercetin 10.0ppm, luteolin 10.0ppm, potassium silicate 77ppm, potassium primary phosphate 0.8%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
According to above-mentioned Nutrition drought resistant agent for plant, the concrete preparation method of described Nutrition drought resistant agent for plant is as follows: to prepare the Nutrition drought resistant agent for plant of 1L,
(1) first according to above-mentioned formula, forulic acid is dissolved in 10mL dehydrated alcohol, then adds vanillic acid, Quercetin and luteolin successively by formula, stir and make it dissolve completely, then add the dilution of 100mL deionized water, make reserve liquid A;
(2) according to above-mentioned formula, Whitfield's ointment is dissolved in 100mL deionized water, then adds potassium silicate, potassium primary phosphate successively respectively, be stirred to it and dissolve completely, make reserve liquid B;
(3) standby being mixed to get of the reserve liquid B that reserve liquid A step (1) obtained and step (2) obtain prepares liquid C;
(4), under stirring, zinc sulfate joined in 100mL deionized water to dissolve completely according to above-mentioned formula and obtain reserve liquid D;
(5) the reserve liquid D that liquid C and step (4) obtain for preparing step (3) obtained mixes, and obtains mixed solution E;
(6) progressively drip anhydrous acetic acid in the mixed solution E obtained to step (5), and rock the container of splendid attire mixed solution E continuously, until the transparent liquid F of solution;
(7) add 2mL tween 80 in the transparent liquid F obtained to step (6), then add deionized water and be settled to 1000mL, obtain solution G;
(8) the solution G that step (7) obtains is the Nutrition drought resistant agent for plant of the application.
the present invention's beneficial effect is compared with prior art:
1. the present invention utilizes phenolic acids to have antioxidants, can improve quantity and the activity of protective enzyme in plant, turn improve the content of polyphenol substance, improves Antioxidants in crop, enhances the drought resistance of crop.Meanwhile, in plant, the raising of phenols Antioxidants can increase crop disease-resistant, uv-resistant radiation etc.
2. the present invention with the addition of potassium primary phosphate, nitrogen in phenolic acid, adds the nutritive element supply of crop, facilitates the production of crop, improve the resistance of crop.
3. the present invention with the addition of element silicon in nutritive medium, adds crop root growth, promotes crop anti-adversity.
4. the present invention is in promotion plant growth, while improving resistance, can increase Antioxidants in wheat grain, improve its nutritional quality.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but do not limit content of the present invention, and wherein involved component is commercially available prod, and involved unit ppm is 1,000,000/.
embodiment 1
A kind of Nutrition drought resistant agent for plant, comprise by the standby solution of the mixed raw material of following concentration expressed in percentage by weight component: Whitfield's ointment 34.8ppm, forulic acid 19.0ppm, vanillic acid 16.8ppm, Quercetin 10.0ppm, luteolin 10.0ppm, potassium silicate 77ppm, potassium primary phosphate 0.5%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
embodiment 2
A kind of Nutrition drought resistant agent for plant, comprise by the standby solution of the mixed raw material of following concentration expressed in percentage by weight component: Whitfield's ointment 55.2ppm, forulic acid 19.0ppm, vanillic acid 16.8ppm, Quercetin 10.0ppm, luteolin 10.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.1%, zinc sulfate 0.55%, surplus is water.
embodiment 3
A kind of Nutrition drought resistant agent for plant, comprise by the standby solution of the mixed raw material of following concentration expressed in percentage by weight component: Whitfield's ointment 20.7ppm, forulic acid 19.0ppm, vanillic acid 16.8ppm, Quercetin 10.0ppm, luteolin 10.0ppm, potassium silicate 77ppm, potassium primary phosphate 0.8%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
embodiment 4
A kind of Nutrition drought resistant agent for plant, comprise by the standby solution of the mixed raw material of following concentration expressed in percentage by weight component: Whitfield's ointment 75.9ppm, forulic acid 19.0ppm, vanillic acid 16.8ppm, Quercetin 10.0ppm, luteolin 10.0ppm, potassium silicate 154ppm, potassium primary phosphate 1.0%, urea 1.2%, zinc sulfate 0.55%, surplus is water.
embodiment 5
A kind of Nutrition drought resistant agent for plant, comprise by the standby solution of the mixed raw material of following concentration expressed in percentage by weight component: Whitfield's ointment 34.8ppm, forulic acid 19.0ppm, vanillic acid 16.8ppm, Quercetin 10.0ppm, luteolin 10.0ppm, potassium silicate 123ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
embodiment 6
A kind of Nutrition drought resistant agent for plant, comprise by the standby solution of the mixed raw material of following concentration expressed in percentage by weight component: Whitfield's ointment 20.7ppm, forulic acid 12.0ppm, vanillic acid 16.8ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
embodiment 7
A kind of Nutrition drought resistant agent for plant, comprise by the standby solution of the mixed raw material of following concentration expressed in percentage by weight component: Whitfield's ointment 34.8ppm, forulic acid 12.0ppm, vanillic acid 16.8ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
embodiment 8
A kind of Nutrition drought resistant agent for plant, comprise by the standby solution of the mixed raw material of following concentration expressed in percentage by weight component: Whitfield's ointment 40.0ppm, forulic acid 12.0ppm, vanillic acid 16.8ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
embodiment 9
A kind of Nutrition drought resistant agent for plant, comprise by the standby solution of the mixed raw material of following concentration expressed in percentage by weight component: Whitfield's ointment 45.0ppm, forulic acid 12.0ppm, vanillic acid 16.8ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
embodiment 10
A kind of Nutrition drought resistant agent for plant, comprise by the standby solution of the mixed raw material of following concentration expressed in percentage by weight component: Whitfield's ointment 55.0ppm, forulic acid 12.0ppm, vanillic acid 16.8ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
embodiment 11
A kind of Nutrition drought resistant agent for plant, comprise by the standby solution of the mixed raw material of following concentration expressed in percentage by weight component: Whitfield's ointment 65.0ppm, forulic acid 12.0ppm, vanillic acid 16.8ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
embodiment 12
A kind of Nutrition drought resistant agent for plant, comprise by the standby solution of the mixed raw material of following concentration expressed in percentage by weight component: Whitfield's ointment 34.8ppm, forulic acid 19.0ppm, vanillic acid 12.0ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 77ppm, potassium primary phosphate 0.5%, urea 1.0%, zinc sulfate 0.35%, surplus is water.
embodiment 13
A kind of Nutrition drought resistant agent for plant, comprise by the standby solution of the mixed raw material of following concentration expressed in percentage by weight component: Whitfield's ointment 20.7ppm, forulic acid 12.0ppm, vanillic acid 12.0ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.35%, surplus is water.
embodiment 14
A kind of Nutrition drought resistant agent for plant, comprise by the standby solution of the mixed raw material of following concentration expressed in percentage by weight component: Whitfield's ointment 55.0ppm, forulic acid 12.0ppm, vanillic acid 12.0ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.35%, surplus is water.
embodiment 15
A kind of Nutrition drought resistant agent for plant, comprise by the standby solution of the mixed raw material of following concentration expressed in percentage by weight component: Whitfield's ointment 65.0ppm, forulic acid 12.0ppm, vanillic acid 12.0ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.35%, surplus is water.
embodiment 16
A kind of Nutrition drought resistant agent for plant, comprise by the standby solution of the mixed raw material of following concentration expressed in percentage by weight component: Whitfield's ointment 34.8ppm, forulic acid 12.0ppm, vanillic acid 12.0ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 123ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.35%, surplus is water.
embodiment 17
A kind of Nutrition drought resistant agent for plant, comprise by the standby solution of the mixed raw material of following concentration expressed in percentage by weight component: Whitfield's ointment 55.2ppm, forulic acid 12.0ppm, vanillic acid 12.0ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 123ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.35%, surplus is water.
embodiment 18
A kind of Nutrition drought resistant agent for plant, comprise by the standby solution of the mixed raw material of following concentration expressed in percentage by weight component: Whitfield's ointment 20.7ppm, forulic acid 12.0ppm, vanillic acid 12.0ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 123ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.55%, surplus is water.
in embodiment 1 ~ 18, the concrete preparation method of Nutrition drought resistant agent for plant is as follows: to prepare the Nutrition drought resistant agent for plant of 1L,
(1) first according to above-mentioned formula, forulic acid is dissolved in 10mL dehydrated alcohol, then adds vanillic acid, Quercetin and luteolin successively by formula, stir and make it dissolve completely, then add the dilution of 100mL deionized water, make reserve liquid A;
(2) according to above-mentioned formula, Whitfield's ointment is dissolved in 100mL deionized water, then adds potassium silicate, potassium primary phosphate successively respectively, be stirred to it and dissolve completely, make reserve liquid B;
(3) standby being mixed to get of the reserve liquid B that reserve liquid A step (1) obtained and step (2) obtain prepares liquid C;
(4), under stirring, zinc sulfate joined in 100mL deionized water to dissolve completely according to above-mentioned formula and obtain reserve liquid D;
(5) the reserve liquid D that liquid C and step (4) obtain for preparing step (3) obtained mixes, and obtains mixed solution E;
(6) progressively drip anhydrous acetic acid in the mixed solution E obtained to step (5), and rock the container of splendid attire mixed solution E continuously, until the transparent liquid F of solution;
(7) add 2mL tween 80 in the transparent liquid F obtained to step (6), then add deionized water and be settled to 1000mL, obtain solution G;
(8) the solution G that step (7) obtains is the Nutrition drought resistant agent for plant of the application, and the pH value of the Nutrition drought resistant agent for plant of gained is 6 ~ 7.
Description involved in following experimental example: rate of emergence is the per-cent % that germination seed quantity accounts for 50 seeds altogether; Height of seedling, plant height are 10 strain sample average height, and unit is centimetre; The longest root length is the length of the longest root in 10 strain samples, and unit is centimetre; Dry-matter fresh weight, dry-matter dry weight, overground part fresh weight are the weight of 6 strain samples, and unit is gram; Chlorophyll content is that Chlorophyll meter SPAD measures numerical value; The unit of total phenol content is micromole/100 gram; The unit of Flavonoid Content is milligram/100 gram; Spike number unit is ten thousand/hectare; Grain number per spike unit is individual; Thousand seed weight is the weight of 1000 wheat seeds, and unit is gram; Output unit is kg/ha.
experimental example 1
This experimental example illustrates that the Nutrition drought resistant agent for plant of embodiment 1 ~ 3 is on the impact of crop bud phase drought resistance under water stress.
Germination test under the wheat 49-198 seed drought stress of wheat breed Henan: choose full wheat (Henan wheat 49-198) seed, through 10% hypochlorite disinfectant 20min, distilled water is cleaned, and adopts respectively
embodiment 1 ~ 3nutrition drought resistant agent for plant seed soaking (contrast distilled water is soaked seed) after 24 hours, putting into diameter is be lined with in the culture dish of filter paper bottom 90mm, and 50, every ware, then adds the respective concentration nutritive medium of 15%PEG-6000, Simulating water stress respectively.Cultivate after 7 days, test-results is in table 1.
As can be seen from Table 1, embodiment 1 ~ 3 is compared contrast, all improves Grain germination rate, and wherein best with embodiment 1, percentage of germination compares according to raising 10 percentage points, and dry matter weight also improves 0.020g.
experimental example 2
This experimental example illustrates that the Nutrition drought resistant agent for plant of embodiment 1, embodiment 4, embodiment 5 is on the impact of crop bud phase drought resistance under water stress.
Germination test under the wheat Zheng wheat 366 seed drought stress of wheat breed Henan: choose full wheat (Zheng wheat 366) seed, through 10% hypochlorite disinfectant 20min, distilled water is cleaned, and adopts respectively
embodiment 1, embodiment 4 and embodiment 5nutrition drought resistant agent for plant seed soaking (contrast distilled water is soaked seed) after 24 hours, putting into diameter is be lined with in the culture dish of filter paper bottom 90mm, and 50, every ware, then adds the respective concentration nutritive medium of 15%PEG-6000, Simulating water stress respectively.Cultivate after 7 days, test-results is in table 2.
As can be seen from Table 2, embodiment 1, embodiment 4, embodiment 5 are compared contrast, all improve Grain germination rate, and wherein best with embodiment 1, percentage of germination compares according to raising 18 percentage points, and dry matter weight also improves 0.020g.Embodiment 4 and embodiment 5 compare and all improve 16 percentage points according to percentage of germination.
experimental example 3
This experimental example illustrates that the Nutrition drought resistant agent for plant of embodiment 6, embodiment 7, embodiment 8, test example 9 and test example 10 is on the impact of crop bud phase drought resistance under water stress.
Germination test under wheat breed western agriculture 979 seed drought stress: choose full wheat (western agriculture 979) seed, through 10% hypochlorite disinfectant 20min, distilled water is cleaned, and adopts respectively
embodiment 6 ~ 10nutrition drought resistant agent for plant seed soaking (contrast distilled water is soaked seed) after 24 hours, putting into diameter is be lined with in the culture dish of filter paper bottom 90mm, and 50, every ware, then adds the respective concentration nutritive medium of 15%PEG-6000, Simulating water stress respectively.Cultivate after 7 days, test-results is in table 3.
As can be seen from Table 3, embodiment 6, embodiment 7, embodiment 8, embodiment 9 are compared with embodiment 10 contrast, all improve Grain germination rate, wherein with embodiment 8 and embodiment 10 more best, percentage of germination compares according to raising 12 percentage points, and dry matter weight also improves 0.019 and 0.018g respectively.
experimental example 4
This experimental example illustrates that the Nutrition drought resistant agent for plant of embodiment 11, embodiment 12, embodiment 13, test example 14 and test example 15 is on the impact of crop bud phase drought resistance under water stress.
Germination test under the short anti-58 seed drought stresses of wheat breed: choose full wheat (short anti-58) seed, through 10% hypochlorite disinfectant 20min, distilled water is cleaned, and adopts respectively
embodiment 11 ~ 15nutrition drought resistant agent for plant seed soaking (contrast distilled water is soaked seed) after 24 hours, putting into diameter is be lined with in the culture dish of filter paper bottom 90mm, and 50, every ware, then adds the respective concentration nutritive medium of 15%PEG-6000, Simulating water stress respectively.Cultivate after 7 days, test-results is in table 4.
As can be seen from Table 4, embodiment 11, embodiment 12, embodiment 13, embodiment 14 are compared with embodiment 15 contrast, all improve Grain germination rate, wherein with embodiment 13 preferred aspect, percentage of germination compares according to raising 13 percentage points, and dry matter weight also improves 0.025g.The percentage of germination of embodiment 15 comparatively contrasts raising 11 percentage points, and dry matter weight increases 0.015g.
experimental example 5
This experimental example illustrates that the Nutrition drought resistant agent for plant of embodiment 16, embodiment 17, embodiment 18 is on the impact of crop bud phase drought resistance under water stress.
Germination test under wheat breed all wheat 27 seeds drought stress: choose full wheat (all wheats 27) seed, through 10% hypochlorite disinfectant 20min, distilled water is cleaned, and adopts respectively
embodiment 16 ~ 18nutrition drought resistant agent for plant seed soaking (contrast distilled water is soaked seed) after 24 hours, putting into diameter is be lined with in the culture dish of filter paper bottom 90mm, and 50, every ware, then adds the respective concentration nutritive medium of 15%PEG-6000, Simulating water stress respectively.Cultivate after 7 days, test-results is in table 5.
As can be seen from Table 5, embodiment 16, embodiment 17, embodiment 18 are compared contrast, all improve Grain germination rate, and wherein with embodiment 16 preferred aspect, percentage of germination compares according to raising 10 percentage points, and dry matter weight also improves 0.017g.The percentage of germination of embodiment 18 comparatively contrasts raising 9 percentage points, and dry matter weight increases 0.015g.
experimental example 6
This experimental example illustrates that the Nutrition drought resistant agent for plant of embodiment 1, embodiment 2 is on the impact of crops seedling stage drought resistance under water stress.
Test adopts water planting mode, the wheat grain (Henan wheat 49-198) after germination is evenly placed in bottom and is lined with in the culture dish of filter paper, be placed in illumination box and cultivate, to 3 leaf phases, carry out the process of lab simulation drought stress to stem and leaf of Wheat.Contrast is for removing the Huo Gelan nutritive medium+20%PEG6000 of nitrogen, phosphorus, potassium, zinc element, and process is respectively
embodiment 1+ 20%PEG6000,
embodiment 2+the Solution culture method of 20%PEG6000.After 6 days, measure plant relative physiologic index.In table 6.
From table 6, drought resisting nutritive medium adds dry matter weight, relative comparison, and fresh weight increases by 0.094 and 0.143g respectively; Chlorophyll content compares according to adding 4.7SPAD value and 1.9SPAD value respectively; Antioxidant total phenol content is 2.45 and 2.14 times of contrast respectively; Flavonoid Content is 1.99 times and 1.88 times of contrast respectively.
experimental example 7
This experimental example illustrates that the Nutrition drought resistant agent for plant of embodiment 1, embodiment 4, embodiment 5 is on the impact of crops seedling stage drought resistance under water stress.
Test adopts husky training mode, and be sowed at by wheat seed (Zheng wheat 366) in the plastic tub of 10cm (diameter) × 10cm (height), basin mesostroma is sand.Wheat 2 leaf 1 heart starts respectively with adopting respectively
embodiment 1,
embodiment 4with
embodiment 53 kinds of drought resistance agent formula solutions carry out spraying process to wheat seedling, and contrast sprays distilled water, starts Osmotic treatment after 5 days, control water is carried out by weighting method, make soil relative water content be 55% (moderate is coerced), control water measured plant height and dry weight after 1 week, the results are shown in Table 7.
From table 7, embodiment 1, embodiment 4, embodiment 5 relative comparison all add plant height and amount of dry matter; Under drought stress, the leaf relative water content spraying drought resisting nutritive medium plant contrast of comparing increases, and what show that drought resisting nutritive medium can alleviate that arid causes wheat plant coerces.
experimental example 8
This experimental example illustrates that the Nutrition drought resistant agent for plant of embodiment 6, embodiment 7, embodiment 8, embodiment 9 is on the impact of crops seedling stage drought resistance under water stress.
Test adopts husky training mode, and be sowed in the plastic tub of 10cm (diameter) × 10cm (height) by wheat seed (western agriculture 979), basin mesostroma is sand.Wheat 2 leaf 1 heart starts respectively with adopting respectively
4 of embodiment 6 ~ 9plant drought resistance agent formula solution and carry out spraying process to wheat seedling, contrast sprays distilled water, starts Osmotic treatment after 5 days, control water is carried out by weighting method, make soil relative water content be 55% (moderate is coerced), control water measured plant height and dry weight after 1 week, the results are shown in Table 8.
From table 8, drought resisting nutrition agent adds plant height and dry matter weight under drought stress, and wherein embodiment 8 relative comparison plant height increases 3.5cm, and overground part fresh weight increases 0.034g, shows that seedling spraying nutritious drought-resisting agent is conducive to alleviating drought stress.
experimental example 9
This experimental example illustrates that the Nutrition drought resistant agent for plant of embodiment 10, embodiment 11, embodiment 12 and embodiment 13 is on the impact of crops seedling stage drought resistance under water stress.
Test adopts water planting mode, the wheat grain (western agriculture 979) after germination is evenly placed in bottom and is lined with in the culture dish of filter paper, be placed in illumination box and cultivate, to 3 leaf phases, carry out the process of lab simulation drought stress to stem and leaf of Wheat.Contrast is for removing the Huo Gelan nutritive medium+20%PEG6000 of nitrogen, phosphorus, potassium, zinc element, and process is respectively
embodiment 10+ 20%PEG6000,
embodiment 11+20%PEG6000,
embodiment 12+20%PEG600,
embodiment 13+the Solution culture method of 20%PEG60000.After 6 days, measure plant relative physiologic index.In table 9.
As shown in Table 9, embodiment 10, embodiment 11, embodiment 12 and embodiment 13 relative comparison plant height on average add 3.4cm, and photograph relative moisture content improves 4 percentage points.
experimental example 10
This experimental example illustrates that the Nutrition drought resistant agent for plant of embodiment 1, embodiment 2, embodiment 3, embodiment 4 is on the impact of plant growth later stage drought resistance.
Test is carried out in test farm, and kind is Henan wheat 49-198, normal sowing in season, wheat plant does not have man-made irrigation the time of infertility, start to spray wheat blade face latter stage in jointing, respectively at jointing stage, full heading time, flowering period, spend within latter 10 days, 20 days, respectively spray once, adopt respectively
embodiment 1 ~ 4spray, each process repeats for 3 times, and grouting measures physical signs mid-term, surveys product after results.Data are in table 10.
From table 10, foliage-spray drought resisting nutrition agent, increase grouting boot leaf chlorophyll content in mid-term, relative comparison, embodiment 1, embodiment 2, embodiment 3 and embodiment 4 increase by 19,16,3.8 and 4.5 respectively; Output then relative comparison per hectare increases by 537 kilograms, 520 kilograms, 402 kilograms and 475.1 kilograms.
experimental example 11
This experimental example illustrates that the Nutrition drought resistant agent for plant of embodiment 5, embodiment 6, embodiment 7, embodiment 8 is on the impact of plant growth later stage drought resistance.
Test is carried out in test farm, and kind is for being permitted section 316, and sowing in normal season, starts to spray wheat blade face in jointing, respectively at jointing stage, full heading time, flowering period, spend within latter 10 days, 20 days, respectively spray once, adopt respectively latter stage
embodiment 5 ~ 8spray, each process repeats for 3 times, and grouting measures physical signs mid-term, and survey product after results, specific experiment result is see table 11.
As shown in Table 11, nutritious drought-resisting agent process relative comparison chlorophyll content on average increases by 4.3, and output on average increases by 278 kilograms.Wherein embodiment 6 relative comparison increases by 430 kilograms, shows that nutritious drought-resisting agent can alleviate arid, increases grain yield.
experimental example 12
This experimental example illustrates that the Nutrition drought resistant agent for plant of embodiment 10, embodiment 11, embodiment 12, embodiment 13 is on the impact of plant growth later stage drought resistance.
Test is carried out in test farm, and wheat breed is all wheats 22, sowing in normal season, starts to spray wheat blade face latter stage, respectively at jointing stage, full heading time, flowering period, spend within latter 10 days, 20 days, respectively spray once, adopt respectively in jointing
embodiment 10 ~ 13spray, each process repeats for 3 times, and grouting measures physical signs mid-term, and survey product after results, specific experiment result is see table 12.
As shown in Table 12, nutritious drought-resisting agent process relative comparison spike number, grain number per spike and thousand seed weight on average increase by 19,0.9 and 2g; Output relative comparison on average adds, and output on average increases by 326 kilograms.Show that nutritious drought-resisting agent can alleviate arid, increase grain yield.
experimental example 13
This experimental example illustrates that the Nutrition drought resistant agent for plant of embodiment 14, embodiment 15, embodiment 16, embodiment 17 and embodiment 18 is on the impact of plant growth later stage drought resistance.
Test is carried out in test farm, and wheat breed is short by anti-58, normal sowing in season, starts to spray wheat blade face latter stage, respectively spray once, adopt respectively respectively at jointing stage, full heading time, flowering period, filling stage in jointing
embodiment 14 ~ 18spray, survey product after results, specific experiment result is see table 13.
As shown in Table 13, spray nutritious drought-resisting agent and improve seed thousand seed weight, on average comparatively contrast increases 2.76g; Output comparatively contrasts average increase by 302.8 kilograms.Wherein embodiment 16 compares other the bests, and output increases by 430.1 kilograms.
experimental example 14
This experimental example illustrates the impact of Nutrition drought resistant agent for plant on plant growth later stage drought resistance and seed antioxidant of embodiment 1, embodiment 2, embodiment 3.
Test is carried out in test farm, and wheat breed is No. 8, safety, sowing in normal season, starts to spray wheat blade face latter stage, respectively spray once, adopt respectively respectively at jointing stage, full heading time, flowering period, filling stage in jointing
embodiment 1 ~ 3spray, survey product after results, specific experiment result is see table 14.
From table 14, embodiment 1, example example 2 and embodiment 3 compare according to volume increase 527 kilograms, 485 kilograms and 425 kilograms; Total phenol content on average increases by 42 micromoles/100g.
Claims (5)
1. a Nutrition drought resistant agent for plant, it is characterized in that: the solution that described Nutrition drought resistant agent for plant is made up of following weight percent concentration component: Whitfield's ointment 20.7ppm ~ 75.9ppm, forulic acid 12.0ppm ~ 19.0ppm, vanillic acid 12.0ppm ~ 16.8ppm, Quercetin 8 ~ 10.0ppm, luteolin 8 ~ 10.0ppm, potassium silicate 77 ~ 154ppm, potassium primary phosphate 0.5 ~ 0.8%, urea 1 ~ 1.2%, zinc sulfate 0.25 ~ 0.55%, surplus is water.
2. Nutrition drought resistant agent for plant according to claim 1, it is characterized in that: the solution be made up of following weight percent concentration component: Whitfield's ointment 34.8ppm, forulic acid 19.0ppm, vanillic acid 16.8ppm, Quercetin 10.0ppm, luteolin 10.0ppm, potassium silicate 77ppm, potassium primary phosphate 0.5%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
3. Nutrition drought resistant agent for plant according to claim 1, it is characterized in that: the solution be made up of following weight percent concentration component: Whitfield's ointment 20.7ppm, forulic acid 19.0ppm, vanillic acid 16.8ppm, Quercetin 10.0ppm, luteolin 10.0ppm, potassium silicate 77ppm, potassium primary phosphate 0.8%, urea 1.0%, zinc sulfate 0.35%, surplus is water.
4. a Nutrition drought resistant agent for plant, it is characterized in that: the solution that described Nutrition drought resistant agent for plant is made up of following weight percent concentration component: Whitfield's ointment 55.2ppm, forulic acid 19.0ppm, vanillic acid 16.8ppm, Quercetin 10.0ppm, luteolin 10.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.1%, zinc sulfate 0.55%, surplus is water.
5. the Nutrition drought resistant agent for plant according to any one of Claims 1 to 4, is characterized in that: the concrete preparation method of described Nutrition drought resistant agent for plant is as follows: to prepare the Nutrition drought resistant agent for plant of 1L,
(1) first according to above-mentioned formula, forulic acid is dissolved in 10mL dehydrated alcohol, then adds vanillic acid, Quercetin and luteolin successively by formula, stir and make it dissolve completely, then add the dilution of 100mL deionized water, make reserve liquid A;
(2) according to above-mentioned formula, Whitfield's ointment is dissolved in 100mL deionized water, then adds potassium silicate, potassium primary phosphate successively respectively, be stirred to it and dissolve completely, make reserve liquid B;
(3) standby being mixed to get of the reserve liquid B that reserve liquid A step (1) obtained and step (2) obtain prepares liquid C;
(4), under stirring, zinc sulfate joined in 100mL deionized water to dissolve completely according to above-mentioned formula and obtain reserve liquid D;
(5) the reserve liquid D that liquid C and step (4) obtain for preparing step (3) obtained mixes, and obtains mixed solution E;
(6) progressively drip anhydrous acetic acid in the mixed solution E obtained to step (5), and rock the container of splendid attire mixed solution E continuously, until the transparent liquid F of solution;
(7) add 2mL tween 80 in the transparent liquid F obtained to step (6), then add deionized water and be settled to 1000mL, obtain solution G;
(8) the solution G that step (7) obtains is Nutrition drought resistant agent for plant.
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| CN104823661A (en) * | 2015-04-29 | 2015-08-12 | 吕梁学院 | Method for increasing drought-resistance capacity of plant |
| CN105060999B (en) * | 2015-09-22 | 2018-08-17 | 江苏农林职业技术学院 | A kind of muskmelon nutrient solution prescription of improving salinization |
| CN106034985A (en) * | 2016-05-31 | 2016-10-26 | 山东胜伟园林科技有限公司 | Method of drip irrigation under plastic film through employing ferulic acid plant protection agent, and application of method in hibiscus planting in saline and alkaline lands |
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| CN106613416A (en) * | 2016-09-29 | 2017-05-10 | 山东省花生研究所 | Method for screening drought resistance type peanuts |
| CN106613116A (en) * | 2016-11-03 | 2017-05-10 | 明光市大全甜叶菊专业合作社 | Stevia rebaudiana old stump transplanting method |
| CN106631354A (en) * | 2016-11-03 | 2017-05-10 | 明光市大全甜叶菊专业合作社 | Stevia rebaudiana stump seedling-raising nutritive water |
| CN109122707A (en) * | 2018-05-10 | 2019-01-04 | 山东省果树研究所 | A kind of plant high temperature and drought adverse-resistant agent and its application |
| CN110771625A (en) * | 2019-09-17 | 2020-02-11 | 江苏新南洋绿色科技有限公司 | Wettable agent formula for enhancing high-temperature stress resistance of crops |
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