CN104003799A - Nutritive drought resistant agent for crops - Google Patents
Nutritive drought resistant agent for crops Download PDFInfo
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- CN104003799A CN104003799A CN201410225514.7A CN201410225514A CN104003799A CN 104003799 A CN104003799 A CN 104003799A CN 201410225514 A CN201410225514 A CN 201410225514A CN 104003799 A CN104003799 A CN 104003799A
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Abstract
The invention relates to a nutritive drought resistant agent for crops, which is capable of increasing the nutrients of the crops and improving the drought resistance of the crops. The nutritive drought resistant agent comprises a solution prepared by mixing the following raw materials in concentration (weight percentage): 13.81-82.8ppm of salicylic acid, 8.0-22.0ppm of ferulic acid, 8.0-20ppm of vanillic acid, 7.0-12.0ppm of quercetin, 7.0-12.0ppm of luteolin, 46.2-184.8ppm of potassium silicate, 0.5-1.0% of potassium dihydrogen phosphate, 1-1.5% of urea, 0.25-0.55% of zinc sulfate and the balance of water. By using the nutritive drought resistant agent, the crop growth can be accelerated, the stress resistance can be improved, meanwhile, the content of antioxidant substances in wheat grains can be increased, and the nutritional quality can be improved.
Description
Technical field
The invention belongs to plant-growth regulator field, be specifically related to a kind of crop alimentary drought resistance agent that strengthens crop alimentary, improves crop drought resistance ability.
Background technology
China's water resources is seriously deficient, is only equivalent to world occupancy volume per person's 1/4, belongs to one of 13Ge poor-water state in the world.Along with increasing of expanding economy and the size of population, to the year two thousand fifty, China is by lack of water 1,000 hundred million m
3, the situation of China's Proposals of Water Resources will be more severe.Drought and water shortage becomes the main limiting factor of China's grain-production, and water deficit is ubiquitous problem in agriculture production, is subject to the impact of physical environment, and most plants are all being stood the threat of scholar's earth water stress to some extent.Wheat is China's staple food crop, and in the face of growing population pressure and the day by day deficient present situation of water resources, study on drought resistance is the important research content in agriculture production always.
Phenols is interior important secondary metabolite in plant.Aldehydes matter in wheat mainly comprises phenolic acid, vanillic acid and some flavonoid materials.Phenolic acid and flavonoids are the important non-enzymatic antioxidants of a class, in the degeneration-resistant process of plant, play a significant role.Study and show under drought condition, in plant body, always the substances content such as phenol, total flavonoid increases, and plant is resisted to drought stress and play an important role.Meanwhile, the flavonoid compound in plant materials has the provide protection of coercing, and can hinder the injuries of abiotic stress to plant materials such as UV, heavy metal, high temperature.Meanwhile, the material such as phenols has obvious effect to plant disease-resistant, germ resistance.Whitfield's ointment is also a kind of simple aldehydes matter producing in plant materials, has multiple effect in plant materials, can promote growth, improves resistance etc.Therefore, utilize that this material is soaked seed, Gen Shi or foliage-spray and other methods of application improve the drought resistance of plant, alleviates the injury of arid to plant, improves the output of crop under adverse circumstance, can provide a new approach for crop drought resistance.
Also have at present some Technology of Drought-resistant to comprise drought resisting preparation, the water-holding agent that more commonly adopts polymer water-absorbent to prepare, as CN98114512.4, but can only emerge for crop.Have the preparation that adopts the anti-transpiration in blade face, as CN201110390028.7, this anti-rising preparation, by pore, suppress rising, but drought resistance is unstable, may suppress leaf photosynthesis simultaneously.Also there are some foliage fertilizers to improve resistance by strengthening plant nutrition, but are mainly at later stage foliage-spray.Although CN201110440703 can spray in seedling stage, also can be at later stage foliage-spray, osmotic adjustment proline(Pro) is mainly leaned in its drought resisting, and in the reaction that proline(Pro) is coerced at Wheat Drought, acts on limited.At present, the patent of relevant concrete phenolic acid in Drought-resistance in Wheat also do not have, and Chinese patent CN101982047 B report utilizes external source phenolic acid to coerce to cultivate the stem and leaf of Wheat that is rich in antioxidant component.Therefore, configure a kind of preparation that there is nutrition and have drought resistance function, not only can promote vine growth and development, there is alleviation drought simultaneously, promote crop yield, be also improved the effect of seed nutritional quality simultaneously.
Summary of the invention
The invention reside in and provide a kind of drought resisting effective, and can improve crop alimentary situation, promote the crop alimentary drought resistance agent of yield and quality.The drought resisting nutrition agent that the compounding that utilization of the present invention contains phenolic acid etc. becomes, according to the different growth phases of crop, adopts respectively the mode of soaking or spraying, and promotes crop growth, increases Antioxidants in crop, improves crop drought resistance.
For realizing above object, the technical solution used in the present invention is:
The invention provides a kind of crop alimentary drought resistance agent, comprise the standby solution forming of mixed raw material by following concentration expressed in percentage by weight component: Whitfield's ointment 13.81ppm～82.8ppm, forulic acid 8.0ppm～22.0ppm, vanillic acid 8.0ppm～20ppm, Quercetin 7.0～12.0ppm, luteolin 7.0～12.0ppm, potassium silicate 46.2～184.8ppm, potassium primary phosphate 0.5～1.0%, urea 1～1.5%, zinc sulfate 0.25～0.55%, surplus is water.
According to above-mentioned crop alimentary drought resistance agent, the solution being formed by following weight percent concentration component: Whitfield's ointment 20.7ppm～75.9ppm, forulic acid 12.0ppm～19.0ppm, vanillic acid 12.0 ppm～16.8ppm, Quercetin 8～10.0ppm, luteolin 8～10.0ppm, potassium silicate 77～154ppm, potassium primary phosphate 0.5～0.8%, urea 1～1.2%, zinc sulfate 0.25～0.55%, surplus is water.
According to above-mentioned crop alimentary drought resistance agent, the solution being formed by following weight percent concentration component: Whitfield's ointment 34.8ppm, forulic acid 19.0ppm, vanillic acid 16.8ppm, Quercetin 10.0ppm, luteolin 10.0ppm, potassium silicate 77ppm, potassium primary phosphate 0.5%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
According to above-mentioned crop alimentary drought resistance agent, the solution being formed by following weight percent concentration component: Whitfield's ointment 55.2ppm, forulic acid 19.0ppm, vanillic acid 16.8ppm, Quercetin 10.0ppm, luteolin 10.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.1%, zinc sulfate 0.55%, surplus is water.
According to above-mentioned crop alimentary drought resistance agent, the solution being formed by following weight percent concentration component: Whitfield's ointment 20.7ppm, forulic acid 19.0ppm, vanillic acid 16.8ppm, Quercetin 10.0ppm, luteolin 10.0ppm, potassium silicate 77ppm, potassium primary phosphate 0.8%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
According to above-mentioned crop alimentary drought resistance agent, the concrete preparation method of described crop alimentary drought resistance agent is as follows: to prepare the crop alimentary drought resistance agent of 1L,
(1) first according to above-mentioned formula, forulic acid is dissolved in 10mL dehydrated alcohol, then by formula, adds successively vanillic acid, Quercetin and luteolin, stir it is dissolved completely, then add the dilution of 100mL deionized water, make reserve liquid A;
(2) according to above-mentioned formula, Whitfield's ointment is dissolved in 100mL deionized water, then adds successively respectively potassium silicate, potassium primary phosphate, be stirred to it and dissolve completely, make reserve liquid B;
(3) standby being mixed to get of reserve liquid B that reserve liquid A step (1) being obtained and step (2) obtain prepared liquid C;
(4) under stirring, according to above-mentioned formula, zinc sulfate is joined in 100mL deionized water and completely dissolves and obtain reserve liquid D;
(5) the reserve liquid D that liquid C and step (4) obtain for preparing step (3) being obtained mixes, and obtains mixed solution E;
(6) in the mixed solution E obtaining to step (5), progressively drip anhydrous acetic acid, and rock continuously the container of splendid attire mixed solution E, until solution is transparent liquid F;
(7) in the transparent liquid F obtaining to step (6), add 2mL tween 80, then add deionized water and be settled to 1000mL, obtain solution G;
(8) the solution G that step (7) obtains is the application's crop alimentary drought resistance agent.
the present invention's beneficial effect is compared with prior art:
1. the present invention utilizes phenolic acids to have antioxidants, can improve quantity and the activity of protective enzyme in plant, has improved again the content of polyphenol substance, improves Antioxidants in crop, has strengthened the drought resistance of crop.Meanwhile, in plant, the raising of phenols Antioxidants can increase crop disease-resistant, anti-UV radiation etc.
2. the present invention has added potassium primary phosphate, nitrogen in phenolic acid, has increased the nutritive element supply of crop, has promoted the production of crop, has improved the resistance of crop.
3. the present invention has added element silicon in nutritive medium, has increased crop root growth, promotes crop anti-adversity.
4. the present invention, when promoting plant growth, improving resistance, can increase Antioxidants in wheat grain, improves its nutritional quality.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but do not limit content of the present invention, and wherein related component is commercially available prod, and the related ppm of unit is 1,000,000/.
embodiment 1
A kind of crop alimentary drought resistance agent, comprise the standby solution forming of mixed raw material by following concentration expressed in percentage by weight component: Whitfield's ointment 34.8ppm, forulic acid 19.0ppm, vanillic acid 16.8ppm, Quercetin 10.0ppm, luteolin 10.0ppm, potassium silicate 77ppm, potassium primary phosphate 0.5%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
embodiment 2
A kind of crop alimentary drought resistance agent, comprise the standby solution forming of mixed raw material by following concentration expressed in percentage by weight component: Whitfield's ointment 55.2ppm, forulic acid 19.0ppm, vanillic acid 16.8ppm, Quercetin 10.0ppm, luteolin 10.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.1%, zinc sulfate 0.55%, surplus is water.
embodiment 3
A kind of crop alimentary drought resistance agent, comprise the standby solution forming of mixed raw material by following concentration expressed in percentage by weight component: Whitfield's ointment 20.7ppm, forulic acid 19.0ppm, vanillic acid 16.8ppm, Quercetin 10.0ppm, luteolin 10.0ppm, potassium silicate 77ppm, potassium primary phosphate 0.8%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
embodiment 4
A kind of crop alimentary drought resistance agent, comprise the standby solution forming of mixed raw material by following concentration expressed in percentage by weight component: Whitfield's ointment 75.9ppm, forulic acid 19.0ppm, vanillic acid 16.8ppm, Quercetin 10.0ppm, luteolin 10.0ppm, potassium silicate 154ppm, potassium primary phosphate 1.0%, urea 1.2%, zinc sulfate 0.55%, surplus is water.
embodiment 5
A kind of crop alimentary drought resistance agent, comprise the standby solution forming of mixed raw material by following concentration expressed in percentage by weight component: Whitfield's ointment 34.8ppm, forulic acid 19.0ppm, vanillic acid 16.8ppm, Quercetin 10.0ppm, luteolin 10.0ppm, potassium silicate 123ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
embodiment 6
A kind of crop alimentary drought resistance agent, comprise the standby solution forming of mixed raw material by following concentration expressed in percentage by weight component: Whitfield's ointment 20.7ppm, forulic acid 12.0ppm, vanillic acid 16.8ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
embodiment 7
A kind of crop alimentary drought resistance agent, comprise the standby solution forming of mixed raw material by following concentration expressed in percentage by weight component: Whitfield's ointment 34.8ppm, forulic acid 12.0ppm, vanillic acid 16.8ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
embodiment 8
A kind of crop alimentary drought resistance agent, comprise the standby solution forming of mixed raw material by following concentration expressed in percentage by weight component: Whitfield's ointment 40.0ppm, forulic acid 12.0ppm, vanillic acid 16.8ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
embodiment 9
A kind of crop alimentary drought resistance agent, comprise the standby solution forming of mixed raw material by following concentration expressed in percentage by weight component: Whitfield's ointment 45.0ppm, forulic acid 12.0ppm, vanillic acid 16.8ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
embodiment 10
A kind of crop alimentary drought resistance agent, comprise the standby solution forming of mixed raw material by following concentration expressed in percentage by weight component: Whitfield's ointment 55.0ppm, forulic acid 12.0ppm, vanillic acid 16.8ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
embodiment 11
A kind of crop alimentary drought resistance agent, comprise the standby solution forming of mixed raw material by following concentration expressed in percentage by weight component: Whitfield's ointment 65.0ppm, forulic acid 12.0ppm, vanillic acid 16.8ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
embodiment 12
A kind of crop alimentary drought resistance agent, comprise the standby solution forming of mixed raw material by following concentration expressed in percentage by weight component: Whitfield's ointment 34.8ppm, forulic acid 19.0ppm, vanillic acid 12.0ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 77ppm, potassium primary phosphate 0.5%, urea 1.0%, zinc sulfate 0.35%, surplus is water.
embodiment 13
A kind of crop alimentary drought resistance agent, comprise the standby solution forming of mixed raw material by following concentration expressed in percentage by weight component: Whitfield's ointment 20.7ppm, forulic acid 12.0ppm, vanillic acid 12.0ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.35%, surplus is water.
embodiment 14
A kind of crop alimentary drought resistance agent, comprise the standby solution forming of mixed raw material by following concentration expressed in percentage by weight component: Whitfield's ointment 55.0ppm, forulic acid 12.0ppm, vanillic acid 12.0ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.35%, surplus is water.
embodiment 15
A kind of crop alimentary drought resistance agent, comprise the standby solution forming of mixed raw material by following concentration expressed in percentage by weight component: Whitfield's ointment 65.0ppm, forulic acid 12.0ppm, vanillic acid 12.0ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.35%, surplus is water.
embodiment 16
A kind of crop alimentary drought resistance agent, comprise the standby solution forming of mixed raw material by following concentration expressed in percentage by weight component: Whitfield's ointment 34.8ppm, forulic acid 12.0ppm, vanillic acid 12.0ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 123ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.35%, surplus is water.
embodiment 17
A kind of crop alimentary drought resistance agent, comprise the standby solution forming of mixed raw material by following concentration expressed in percentage by weight component: Whitfield's ointment 55.2ppm, forulic acid 12.0ppm, vanillic acid 12.0ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 123ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.35%, surplus is water.
embodiment 18
A kind of crop alimentary drought resistance agent, comprise the standby solution forming of mixed raw material by following concentration expressed in percentage by weight component: Whitfield's ointment 20.7ppm, forulic acid 12.0ppm, vanillic acid 12.0ppm, Quercetin 8.0ppm, luteolin 8.0ppm, potassium silicate 123ppm, potassium primary phosphate 1.0%, urea 1.0%, zinc sulfate 0.55%, surplus is water.
in embodiment 1 ~ 18, the concrete preparation method of crop alimentary drought resistance agent is as follows: to prepare the crop alimentary drought resistance agent of 1L,
(1) first according to above-mentioned formula, forulic acid is dissolved in 10mL dehydrated alcohol, then by formula, adds successively vanillic acid, Quercetin and luteolin, stir it is dissolved completely, then add the dilution of 100mL deionized water, make reserve liquid A;
(2) according to above-mentioned formula, Whitfield's ointment is dissolved in 100mL deionized water, then adds successively respectively potassium silicate, potassium primary phosphate, be stirred to it and dissolve completely, make reserve liquid B;
(3) standby being mixed to get of reserve liquid B that reserve liquid A step (1) being obtained and step (2) obtain prepared liquid C;
(4) under stirring, according to above-mentioned formula, zinc sulfate is joined in 100mL deionized water and completely dissolves and obtain reserve liquid D;
(5) the reserve liquid D that liquid C and step (4) obtain for preparing step (3) being obtained mixes, and obtains mixed solution E;
(6) in the mixed solution E obtaining to step (5), progressively drip anhydrous acetic acid, and rock continuously the container of splendid attire mixed solution E, until solution is transparent liquid F;
(7) in the transparent liquid F obtaining to step (6), add 2mL tween 80, then add deionized water and be settled to 1000mL, obtain solution G;
(8) the solution G that step (7) obtains is the application's crop alimentary drought resistance agent, and the pH value of the crop alimentary drought resistance agent of gained is 6～7.
Related description in following experimental example: rate of emergence is that germination seed quantity accounts for the per-cent % of 50 seeds altogether; Height of seedling, plant height are 10 strain sample average height, and unit is centimetre; The longest root length is the length of long root in 10 strain samples, and unit is centimetre; Dry-matter fresh weight, dry-matter dry weight, overground part fresh weight are the weight of 6 strain samples, and unit is gram; Chlorophyll content is that Chlorophyll meter SPAD measures numerical value; The unit of total phenol content is micromole/100 gram; The unit of Flavonoid Content be milligram/100 grams; Spike number unit is ten thousand/hectare; Grain number per spike unit is individual; Thousand seed weight is the weight of 1000 wheat seeds, and unit is gram; Output unit is kg/ha.
experimental example 1
The impact of the crop alimentary drought resistance agent of this experimental example explanation embodiment 1 ~ 3 on crop bud phase drought resistance under water stress.
Germination test under the wheat 49-198 seed drought stress of wheat breed Henan: choose full wheat (Henan wheat 49-198) seed, through 10% clorox sterilization 20min, distilled water is cleaned, and adopts respectively
embodiment 1 ~ 3crop alimentary drought resistance agent seed soaking (contrast is soaked seed with distilled water) after 24 hours, putting into diameter is that 90mm bottom is lined with in the culture dish of filter paper, then 50, every ware adds respectively the respective concentration nutritive medium of 15% PEG-6000, simulation water stress.Cultivate after 7 days, test-results is in Table 1.
As can be seen from Table 1, embodiment 1～3 contrast of comparing, has all improved seed percentage of germination, and wherein with embodiment 1 the best, percentage of germination compares according to improving 10 percentage points, and dry matter weight has also improved 0.020g.
experimental example 2
The impact of the crop alimentary drought resistance agent of the explanation of this experimental example embodiment 1, embodiment 4, embodiment 5 on crop bud phase drought resistance under water stress.
Germination test under the wheat Zheng wheat 366 seed drought stresses of wheat breed Henan: choose full wheat (Zheng wheat 366) seed, through 10% clorox sterilization 20min, distilled water is cleaned, and adopts respectively
embodiment 1, embodiment 4 and embodiment 5crop alimentary drought resistance agent seed soaking (contrast is soaked seed with distilled water) after 24 hours, putting into diameter is that 90mm bottom is lined with in the culture dish of filter paper, then 50, every ware adds respectively the respective concentration nutritive medium of 15% PEG-6000, simulation water stress.Cultivate after 7 days, test-results is in Table 2.
As can be seen from Table 2, embodiment 1, embodiment 4, embodiment 5 contrast of comparing, has all improved seed percentage of germination, and wherein with embodiment 1 the best, percentage of germination compares according to improving 18 percentage points, and dry matter weight has also improved 0.020g.Embodiment 4 and embodiment 5 compare according to percentage of germination and have all improved 16 percentage points.
experimental example 3
?the impact of the crop alimentary drought resistance agent of the explanation of this experimental example embodiment 6, embodiment 7, embodiment 8, test example 9 and test example 10 on crop bud phase drought resistance under water stress.
Germination test under the western agriculture 979 seed drought stresses of wheat breed: choose full wheat (western agriculture 979) seed, through 10% clorox sterilization 20min, distilled water is cleaned, and adopts respectively
embodiment 6 ~ 10crop alimentary drought resistance agent seed soaking (contrast is soaked seed with distilled water) after 24 hours, putting into diameter is that 90mm bottom is lined with in the culture dish of filter paper, then 50, every ware adds respectively the respective concentration nutritive medium of 15% PEG-6000, simulation water stress.Cultivate after 7 days, test-results is in Table 3.
As can be seen from Table 3, embodiment 6, embodiment 7, embodiment 8, embodiment 9 and embodiment 10 contrast of comparing, all improved seed percentage of germination, wherein more best with embodiment 8 and embodiment 10, percentage of germination compares according to improving 12 percentage points, and dry matter weight has also improved respectively 0.019 and 0.018g.
experimental example 4
?the impact of the crop alimentary drought resistance agent of the explanation of this experimental example embodiment 11, embodiment 12, embodiment 13, test example 14 and test example 15 on crop bud phase drought resistance under water stress.
Germination test under the short anti-58 seed drought stresses of wheat breed: choose full wheat (short anti-58) seed, through 10% clorox sterilization 20min, distilled water is cleaned, and adopts respectively
embodiment 11 ~ 15crop alimentary drought resistance agent seed soaking (contrast is soaked seed with distilled water) after 24 hours, putting into diameter is that 90mm bottom is lined with in the culture dish of filter paper, then 50, every ware adds respectively the respective concentration nutritive medium of 15% PEG-6000, simulation water stress.Cultivate after 7 days, test-results is in Table 4.
As can be seen from Table 4, embodiment 11, embodiment 12, embodiment 13, embodiment 14 and embodiment 15 contrast of comparing, has all improved seed percentage of germination, wherein with embodiment 13 preferred aspect, percentage of germination compares according to improving 13 percentage points, and dry matter weight has also improved 0.025g.The percentage of germination of embodiment 15 contrasts and improves 11 percentage points, and dry matter weight increases 0.015g.
experimental example 5
The impact of the crop alimentary drought resistance agent of the explanation of this experimental example embodiment 16, embodiment 17, embodiment 18 on crop bud phase drought resistance under water stress.
Germination test under all wheat 27 seed drought stresses of wheat breed: choose full wheat (all wheats 27) seed, through 10% clorox sterilization 20min, distilled water is cleaned, and adopts respectively
embodiment 16 ~ 18crop alimentary drought resistance agent seed soaking (contrast is soaked seed with distilled water) after 24 hours, putting into diameter is that 90mm bottom is lined with in the culture dish of filter paper, then 50, every ware adds respectively the respective concentration nutritive medium of 15% PEG-6000, simulation water stress.Cultivate after 7 days, test-results is in Table 5.
As can be seen from Table 5, embodiment 16, embodiment 17, embodiment 18 contrast of comparing, has all improved seed percentage of germination, and wherein with embodiment 16 preferred aspect, percentage of germination compares according to improving 10 percentage points, and dry matter weight has also improved 0.017g.The percentage of germination of embodiment 18 contrasts and improves 9 percentage points, and dry matter weight increases 0.015g.
experimental example 6
The impact of the crop alimentary drought resistance agent of the explanation of this experimental example embodiment 1, embodiment 2 on crops seedling stage drought resistance under water stress.
Test adopts water planting mode, the wheat grain (Henan wheat 49-198) after germinateing is evenly placed in to bottom and is lined with in the culture dish of filter paper, is placed in illumination box and cultivates, and to 3 leaf phases, stem and leaf of Wheat is carried out to the processing of lab simulation drought stress.Contrast is for removing the Huo Gelan nutritive medium+20%PEG6000 of nitrogen, phosphorus, potassium, zinc element, and processing is respectively
embodiment 1+ 20% PEG6000,
embodiment 2+the nutritive medium of 20% PEG6000 is cultivated.After 6 days, measure plant relative physiologic index.In Table 6.
From table 6, drought resisting nutritive medium has increased dry matter weight, relative comparison, and fresh weight increases respectively 0.094 and 0.143g; Chlorophyll content compares according to having increased respectively 4.7SPAD value and 1.9SPAD value; Antioxidant total phenol content is respectively 2.45 and 2.14 times of contrast; Flavonoid Content is respectively 1.99 times and 1.88 times of contrast.
experimental example 7
The impact of the crop alimentary drought resistance agent of the explanation of this experimental example embodiment 1, embodiment 4, embodiment 5 on crops seedling stage drought resistance under water stress.
Test adopts husky training mode, and wheat seed (Zheng wheat 366) is sowed in the plastic tub of 10cm (diameter) * 10cm (height), and basin mesostroma is sand.Wheat 2 leaf 1 hearts start respectively with adopting respectively
embodiment 1,
embodiment 4with
embodiment 53 kinds of drought resistance agent formula solutions are to the wheat seedling processing of spraying, and contrast sprays distilled water, starts arid and process after 5 days, by weighting method, control water, making soil relative water content is 55% (moderate is coerced), and control water is measured plant height and dry weight after 1 week, the results are shown in Table 7.
From table 7, embodiment 1, embodiment 4, embodiment 5 relative comparisons have all increased plant height and amount of dry matter; Under drought stress, the leaf relative water content that the sprays drought resisting nutritive medium plant contrast of comparing increases, and shows that drought resisting nutritive medium can alleviate coercing that arid causes wheat plant.
experimental example 8
The impact of the crop alimentary drought resistance agent of the explanation of this experimental example embodiment 6, embodiment 7, embodiment 8, embodiment 9 on crops seedling stage drought resistance under water stress.
Test adopts husky training mode, and wheat seed (western agriculture 979) is sowed in the plastic tub of 10cm (diameter) * 10cm (height), and basin mesostroma is sand.Wheat 2 leaf 1 hearts start respectively with adopting respectively
4 of embodiment 6～9kind of drought resistance agent formula solution is to wheat seedling processings of spray, and contrast sprays distilled water, starts arid processing after 5 days, by weighting method, control water, making soil relative water content is 55% (moderate is coerced), and control water is measured plant height and dry weight after 1 week, the results are shown in Table 8.
From table 8, drought resisting nutrition agent has increased plant height and dry matter weight under drought stress, and wherein embodiment 8 relative comparison plant heights increase 3.5cm, and overground part fresh weight increases 0.034g, shows that seedling spraying nutritious drought-resisting agent is conducive to alleviate drought stress.
experimental example 9
The impact of the crop alimentary drought resistance agent of the explanation of this experimental example embodiment 10, embodiment 11, embodiment 12 and embodiment 13 on crops seedling stage drought resistance under water stress.
Test adopts water planting mode, the wheat grain (western agriculture 979) after germinateing is evenly placed in to bottom and is lined with in the culture dish of filter paper, is placed in illumination box and cultivates, and to 3 leaf phases, stem and leaf of Wheat is carried out to the processing of lab simulation drought stress.Contrast is for removing the Huo Gelan nutritive medium+20%PEG6000 of nitrogen, phosphorus, potassium, zinc element, and processing is respectively
embodiment 10+ 20% PEG6000,
embodiment 11+20% PEG6000,
embodiment 12+20% PEG600,
embodiment 13+the nutritive medium of 20% PEG60000 is cultivated.After 6 days, measure plant relative physiologic index.In Table 9.
As shown in Table 9, embodiment 10, embodiment 11, embodiment 12 and embodiment 13 relative comparison plant heights have on average increased 3.4cm, and photograph relative moisture content has improved 4 percentage points.
experimental example 10
The impact of the crop alimentary drought resistance agent of the explanation of this experimental example embodiment 1, embodiment 2, embodiment 3, embodiment 4 on plant growth later stage drought resistance.
Test is carried out in test farm, and kind is Henan wheat 49-198, normal sowing in season, wheat plant does not have man-made irrigation the time of infertility, in jointing, start wheat blade face to spray latter stage, respectively at jointing stage, full heading time, flowering period, spend within latter 10 days, 20 days, respectively spray once, adopt respectively
embodiment 1～4spray, each is processed 3 times and repeats, and mid-term is measured physical signs in grouting, after results, surveys and produces.Data are in Table 10.
From table 10, foliage-spray drought resisting nutrition agent, increases grouting boot leaf chlorophyll content in mid-term, relative comparison, and embodiment 1, embodiment 2, embodiment 3 and embodiment 4 increase respectively 19,16,3.8 and 4.5; Output relative comparison per hectare increases by 537 kilograms, 520 kilograms, 402 kilograms and 475.1 kilograms.
experimental example 11
The impact of the crop alimentary drought resistance agent of the explanation of this experimental example embodiment 5, embodiment 6, embodiment 7, embodiment 8 on plant growth later stage drought resistance.
Test is carried out in test farm, kind Wei Xu section 316, and normal sowing in season, starts wheat blade face to spray in jointing latter stage, respectively at jointing stage, full heading time, flowering period, spend within latter 10 days, 20 days, respectively spray once, adopts respectively
embodiment 5～8spray, each is processed 3 times and repeats, and mid-term is measured physical signs in grouting, after results, surveys and produces, and specific experiment result is referring to table 11.
As shown in Table 11, nutritious drought-resisting agent is processed relative comparison chlorophyll content on average increases by 4.3, and output on average increases by 278 kilograms.Wherein embodiment 6 relative comparisons increase by 430 kilograms, show that nutritious drought-resisting agent can alleviate arid, increase grain yield.
experimental example 12
The impact of the crop alimentary drought resistance agent of the explanation of this experimental example embodiment 10, embodiment 11, embodiment 12, embodiment 13 on plant growth later stage drought resistance.
Test is carried out in test farm, and wheat breed is all wheats 22, and normal sowing in season starts wheat blade face to spray in jointing latter stage, respectively at jointing stage, full heading time, flowering period, spend within latter 10 days, 20 days, respectively spray once, adopts respectively
embodiment 10～13spray, each is processed 3 times and repeats, and mid-term is measured physical signs in grouting, after results, surveys and produces, and specific experiment result is referring to table 12.
As shown in Table 12, nutritious drought-resisting agent is processed relative comparison spike number, grain number per spike and thousand seed weight on average increases by 19,0.9 and 2g; Output relative comparison has on average increased, and output on average increases by 326 kilograms.Show that nutritious drought-resisting agent can alleviate arid, increase grain yield.
experimental example 13
The impact of the crop alimentary drought resistance agent of the explanation of this experimental example embodiment 14, embodiment 15, embodiment 16, embodiment 17 and embodiment 18 on plant growth later stage drought resistance.
Test is carried out in test farm, and wheat breed is short anti-58, and normal sowing in season starts wheat blade face to spray in jointing latter stage, respectively sprays once respectively at jointing stage, full heading time, flowering period, filling stage, adopts respectively
embodiment 14～18spray, after results, survey and produce, specific experiment result is referring to table 13.
As shown in Table 13, spray nutritious drought-resisting agent and improved seed thousand seed weight, on average contrast increases 2.76g; Output contrasts 302.8 kilograms of average increases.Wherein embodiment 16 compares other the bests, and output increases by 430.1 kilograms.
experimental example 14
The impact of the crop alimentary drought resistance agent of the explanation of this experimental example embodiment 1, embodiment 2, embodiment 3 on plant growth later stage drought resistance and seed antioxidant.
Test is carried out in test farm, and wheat breed is No. 8, safety, and normal sowing in season starts wheat blade face to spray in jointing latter stage, respectively sprays once respectively at jointing stage, full heading time, flowering period, filling stage, adopts respectively
embodiment 1～3spray, after results, survey and produce, specific experiment result is referring to table 14.
From table 14, embodiment 1, example example 2 and embodiment 3 compare according to 527 kilograms, 485 kilograms and 425 kilograms of volume increase; Total phenol content on average increases by 42 micromoles/100g.
Claims (6)
1. a crop alimentary drought resistance agent, it is characterized in that, comprise the standby solution forming of mixed raw material by following concentration expressed in percentage by weight component: Whitfield's ointment 13.81ppm～82.8ppm, forulic acid 8.0ppm～22.0ppm, vanillic acid 8.0ppm～20ppm, Quercetin 7.0～12.0ppm, luteolin 7.0～12.0ppm, potassium silicate 46.2～184.8ppm, potassium primary phosphate 0.5～1.0%, urea 1～1.5%, zinc sulfate 0.25～0.55%, surplus is water.
2. crop alimentary drought resistance agent according to claim 1, it is characterized in that: the solution being formed by following weight percent concentration component: Whitfield's ointment 20.7ppm～75.9ppm, forulic acid 12.0ppm～19.0ppm, vanillic acid 12.0 ppm～16.8ppm, Quercetin 8～10.0ppm, luteolin 8～10.0ppm, potassium silicate 77～154ppm, potassium primary phosphate 0.5～0.8%, urea 1～1.2%, zinc sulfate 0.25～0.55%, surplus is water.
3. crop alimentary drought resistance agent according to claim 1, it is characterized in that: the solution being formed by following weight percent concentration component: Whitfield's ointment 34.8ppm, forulic acid 19.0ppm, vanillic acid 16.8ppm, Quercetin 10.0ppm, luteolin 10.0ppm, potassium silicate 77ppm, potassium primary phosphate 0.5%, urea 1.0%, zinc sulfate 0.25%, surplus is water.
4. crop alimentary drought resistance agent according to claim 1, it is characterized in that: the solution being formed by following weight percent concentration component: Whitfield's ointment 55.2ppm, forulic acid 19.0ppm, vanillic acid 16.8ppm, Quercetin 10.0ppm, luteolin 10.0ppm, potassium silicate 77ppm, potassium primary phosphate 1.0%, urea 1.1%, zinc sulfate 0.55%, surplus is water.
5. crop alimentary drought resistance agent according to claim 1, it is characterized in that: the solution being formed by following weight percent concentration component: Whitfield's ointment 20.7ppm, forulic acid 19.0ppm, vanillic acid 16.8ppm, Quercetin 10.0ppm, luteolin 10.0ppm, potassium silicate 77ppm, potassium primary phosphate 0.8%, urea 1.0%, zinc sulfate 0.35%, surplus is water.
6. according to the crop alimentary drought resistance agent described in claim 1～5 any one, it is characterized in that: the concrete preparation method of described crop alimentary drought resistance agent is as follows: to prepare the crop alimentary drought resistance agent of 1L,
(1) first according to above-mentioned formula, forulic acid is dissolved in 10mL dehydrated alcohol, then by formula, adds successively vanillic acid, Quercetin and luteolin, stir it is dissolved completely, then add the dilution of 100mL deionized water, make reserve liquid A;
(2) according to above-mentioned formula, Whitfield's ointment is dissolved in 100mL deionized water, then adds successively respectively potassium silicate, potassium primary phosphate, be stirred to it and dissolve completely, make reserve liquid B;
(3) standby being mixed to get of reserve liquid B that reserve liquid A step (1) being obtained and step (2) obtain prepared liquid C;
(4) under stirring, according to above-mentioned formula, zinc sulfate is joined in 100mL deionized water and completely dissolves and obtain reserve liquid D;
(5) the reserve liquid D that liquid C and step (4) obtain for preparing step (3) being obtained mixes, and obtains mixed solution E;
(6) in the mixed solution E obtaining to step (5), progressively drip anhydrous acetic acid, and rock continuously the container of splendid attire mixed solution E, until solution is transparent liquid F;
(7) in the transparent liquid F obtaining to step (6), add 2mL tween 80, then add deionized water and be settled to 1000mL, obtain solution G;
(8) the solution G that step (7) obtains is the application's crop alimentary drought resistance agent.
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