CN106359451A - Compound conditioning agent for delaying senility of leaves of wheat and/or improving yield, medicament comprising same, and application of same - Google Patents
Compound conditioning agent for delaying senility of leaves of wheat and/or improving yield, medicament comprising same, and application of same Download PDFInfo
- Publication number
- CN106359451A CN106359451A CN201610727534.3A CN201610727534A CN106359451A CN 106359451 A CN106359451 A CN 106359451A CN 201610727534 A CN201610727534 A CN 201610727534A CN 106359451 A CN106359451 A CN 106359451A
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- China
- Prior art keywords
- wheat
- composite regulator
- water
- salicylic acid
- surplus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 74
- 241000209140 Triticum Species 0.000 title claims abstract description 19
- 239000003814 drug Substances 0.000 title claims abstract description 15
- 239000003795 chemical substances by application Substances 0.000 title abstract description 5
- 150000001875 compounds Chemical class 0.000 title abstract description 5
- 230000003750 conditioning effect Effects 0.000 title abstract 4
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 claims abstract description 66
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 47
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 43
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 33
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 claims abstract description 33
- 229960004889 salicylic acid Drugs 0.000 claims abstract description 33
- XEYBHCRIKKKOSS-UHFFFAOYSA-N disodium;azanylidyneoxidanium;iron(2+);pentacyanide Chemical compound [Na+].[Na+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].[O+]#N XEYBHCRIKKKOSS-UHFFFAOYSA-N 0.000 claims abstract description 32
- 229940083618 sodium nitroprusside Drugs 0.000 claims abstract description 32
- 230000000694 effects Effects 0.000 claims abstract description 24
- 239000002131 composite material Substances 0.000 claims description 84
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 32
- 230000000243 photosynthetic effect Effects 0.000 claims description 19
- 239000000203 mixture Substances 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 13
- 210000000582 semen Anatomy 0.000 claims description 13
- 230000009758 senescence Effects 0.000 claims description 11
- 229930002875 chlorophyll Natural products 0.000 claims description 9
- 235000019804 chlorophyll Nutrition 0.000 claims description 9
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 claims description 9
- 239000007921 spray Substances 0.000 claims description 9
- 102000016938 Catalase Human genes 0.000 claims description 6
- 108010053835 Catalase Proteins 0.000 claims description 6
- 102000019197 Superoxide Dismutase Human genes 0.000 claims description 6
- 108010012715 Superoxide dismutase Proteins 0.000 claims description 6
- 230000012010 growth Effects 0.000 claims description 4
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 abstract 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 abstract 1
- 244000098338 Triticum aestivum Species 0.000 description 59
- 238000002360 preparation method Methods 0.000 description 22
- 241000196324 Embryophyta Species 0.000 description 14
- 238000012360 testing method Methods 0.000 description 11
- 239000000463 material Substances 0.000 description 6
- 235000013339 cereals Nutrition 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 150000002500 ions Chemical class 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 241000894007 species Species 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 4
- 102000004139 alpha-Amylases Human genes 0.000 description 4
- 108090000637 alpha-Amylases Proteins 0.000 description 4
- 229940024171 alpha-amylase Drugs 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 241000282326 Felis catus Species 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 239000008187 granular material Substances 0.000 description 3
- 239000013618 particulate matter Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 241000209136 Agropyron Species 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 230000035508 accumulation Effects 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 244000037666 field crops Species 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 229930195732 phytohormone Natural products 0.000 description 2
- 230000037039 plant physiology Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000008213 purified water Substances 0.000 description 2
- -1 salicylic acid (sa) Natural products 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- JLIDBLDQVAYHNE-LXGGSRJLSA-N 2-cis-abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\C1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-LXGGSRJLSA-N 0.000 description 1
- LWFUFLREGJMOIZ-UHFFFAOYSA-N 3,5-dinitrosalicylic acid Chemical compound OC(=O)C1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1O LWFUFLREGJMOIZ-UHFFFAOYSA-N 0.000 description 1
- 241000209758 Aegilops Species 0.000 description 1
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Natural products OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000228648 Dasypyrum Species 0.000 description 1
- 241001523362 Eremopyrum Species 0.000 description 1
- 241000122202 Haynaldia Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- WAEMQWOKJMHJLA-UHFFFAOYSA-N Manganese(2+) Chemical compound [Mn+2] WAEMQWOKJMHJLA-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241001523344 Pseudoroegneria Species 0.000 description 1
- 241000209056 Secale Species 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 241001318343 Triticinae Species 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- RJGDLRCDCYRQOQ-UHFFFAOYSA-N anthrone Chemical compound C1=CC=C2C(=O)C3=CC=CC=C3CC2=C1 RJGDLRCDCYRQOQ-UHFFFAOYSA-N 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- SEGLCEQVOFDUPX-UHFFFAOYSA-N di-(2-ethylhexyl)phosphoric acid Chemical compound CCCCC(CC)COP(O)(=O)OCC(CC)CCCC SEGLCEQVOFDUPX-UHFFFAOYSA-N 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910001437 manganese ion Inorganic materials 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229910000000 metal hydroxide Inorganic materials 0.000 description 1
- 229910044991 metal oxide Inorganic materials 0.000 description 1
- 150000004706 metal oxides Chemical class 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 238000005502 peroxidation Methods 0.000 description 1
- 239000008177 pharmaceutical agent Substances 0.000 description 1
- 229940085991 phosphate ion Drugs 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 229960003975 potassium Drugs 0.000 description 1
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000003044 randomized block design Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000009711 regulatory function Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 230000009889 stress physiology Effects 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000002349 well water Substances 0.000 description 1
- 235000020681 well water Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/26—Phosphorus; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/24—Cyanogen or compounds thereof, e.g. hydrogen cyanide, cyanic acid, cyanamide, thiocyanic acid
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a compound conditioning agent for delaying senility of leaves of wheat and/or improving yield, an application of same and a medicament comprising same, wherein 1 kg of the compound conditioning agent includes: 20-40 mg of sodium nitroprusside, 200-400 mg of salicylic acid, 22000-50000 mg of monopotassium phosphate, and the balanced being water. The compound conditioning agent can delay the senility of leaves of wheat and/or improve the yield of wheat, and has excellent technical effects.
Description
Technical field
The present invention relates to a kind of composite regulator delaying Wheat Leaves Senescence and/or improving wheat yield, belong to plant
Regulator field.
Background technology
Huang-Huai-Hai is the most important producing region of China's Semen Tritici aestivi, and this area's Growth of Wheat has tillering stage length, young fringe divides
Change phase length and the feature of pustulation period short (" two long one is short "), thus this area's wheat tillering disclosure satisfy that mature spike number demand substantially, and
Spend more many with the obvious advantage, advantageously form many of big fringe, but due to Huang-Huai-Hai Wheat Plant in Late Filling Period often occur unfavorable
Weather, especially high temperature, arid, leaf chlorophyll enzyme, Lipid peroxidation and protective enzyme system superoxide dismutase
(sod), catalase (cat), peroxidase and ascorbic acid oxidase isoreactivity change, and cause leaf photosynthesis energy
Power declines and leaf senile.Due to pustulation period seed starch synthesis mainly with blade synthesis photosynthate as raw material, because
If this pustulation period meets adverse environmental factor, in many wheat breed seeds, the dynamic accumulation of starch is restricted, and causes these kinds high
Grain weight potentiality are difficult to be not fully exerted, thus constrain the raising further of yield.
The mass of 1000 kernel potentiality of Huang-Huai-Hai spread wheat breed are mostly in more than 48g now, but actual production
In, throughout the year in below 42g, plumpness is poor for the mass of 1000 kernel of these kinds, therefore, only this index of grain weight, these kinds increase
Produce potentiality up to 60kg/667m2(test summarized materials according to Winter Wheat In Henan Province kind region 2008-2015, with thousand above
Grain weight often increases 1g, every 667m2Increase 10kg to calculate).Therefore, improving Grain Weight in Common Wheat is the important channel improving yield.
Nitric oxide (nitric oxide, no) is a kind of bioactive molecule and the plant life of latest find in plant body
Long Auto-regulator (cevahir etc., 2003).When by environment stress, Plant emission goes out no and participates in photosynthetic regulation and control,
Thus improving resistance become one of hot issue of domestic and international plant stress physiology research.Phytohormone is to growth metabolism
Regulation be complicated, also generally existing (the pospisilova et al.2005) of the mutual effect and between other Auto-regulator.
Research shows no and other phytohormone such as salicylic acid (sa), abscisic acid (aba), jasmonic (ja), h2o2There is mutually collaborative work
With, improve activities of antioxidant enzymes reduce active oxygen produce thus protect Photosynthetic from stress injury (Zhang Yikai etc.,
2010).
Some plants all can be significantly improved with regard to this two classes material of no and sa and its functional analogue to biotic and non-
The resistance of biotic, increases crop yield, and low production cost, nontoxic, noresidue, shows huge in production practices
Application potential.But delay Wheat Leaves Senescence about this two classes Auto-regulator, there is not been reported for the research of raising wheat yield.For
This, this experimental study the two improve the effect of wheat yield, delay Wheat Leaves Senescence and improve Semen Tritici aestivi thousand to develop
Weight and the composite regulator of yield.
Content of the invention
The technical problem to be solved is to provide one kind to delay Wheat Leaves Senescence for the deficiencies in the prior art
And/or improve the composite regulator of wheat yield.
An aspect of of the present present invention, provides a kind of composite regulator, and the composition of every kilogram of described composite regulator is as follows: nitre is general
Sodium (snp) 20~40mg;Salicylic acid 200~400mg;Potassium dihydrogen phosphate 22000~50000mg;Water with surplus.
In a preferred embodiment, the composite regulator of the present invention, based on composite regulator every kilogram described, its composition
Any one of it is preferably following 1)~8):
1) sodium nitroprusside 20~30mg;Salicylic acid 200~300mg;Potassium dihydrogen phosphate 22000~35000mg;With surplus
Water;
2) sodium nitroprusside 30~40mg;Salicylic acid 200~400mg;Potassium dihydrogen phosphate 35000~50000mg;With surplus
Water;
3) sodium nitroprusside 20mg;Salicylic acid 400mg;Potassium dihydrogen phosphate 50000mg;Water with surplus;
4) sodium nitroprusside 30mg;Salicylic acid 400mg;Potassium dihydrogen phosphate 50000mg;Water with surplus;
5) sodium nitroprusside 40mg;Salicylic acid 200~300mg;Potassium dihydrogen phosphate 35000~50000mg;Water with surplus;
6) sodium nitroprusside 30~40mg;Salicylic acid 200~300mg;Potassium dihydrogen phosphate 35000~50000mg;With surplus
Water;
7) sodium nitroprusside 20~30mg;Salicylic acid 300~400mg;Potassium dihydrogen phosphate 35000~50000mg;With surplus
Water;
8) sodium nitroprusside 30~40mg;Salicylic acid 300~400mg;Potassium dihydrogen phosphate 35000~50000mg;With surplus
Water.
Another aspect of the present invention, also provides application in adjusting wheat growth for the described composite regulator.Preferred
In embodiment, can be used for delaying Wheat Leaves Senescence and/or improve wheat yield.For example, be selected from following any one
In application:
(1) improve wheat leaf blade chlorophyll content
(2) improve wheat leaf blade photosynthetic rate
(3) improve Photosynthetic Function of Wheat Leaf index
(4) increase wheat leaf blade superoxide dismutase activity
(5) increase wheat leaf blade catalase activity
(6) improve wheat seed mass of 1000 kernel
(7) increase wheat yield.
In a preferred embodiment, preferably in the following manner using described compound in the above-mentioned application of the present invention
Regulator:
Use described composite regulator by way of seed dressing or in the mode that Semen Tritici aestivi spends spray for latter 15-20 days, more excellent
The mode that both choosings are used in combination.
Another aspect of the invention, provides a kind of medicament delaying Wheat Leaves Senescence and/or improving wheat yield, its bag
Containing composite regulator of the present invention.
Another aspect of the present invention, provides a kind of method delaying Wheat Leaves Senescence and/or improving wheat yield, wherein
Using composite regulator of the present invention.
Show through the test of pesticide effectiveness, the present invention provide composite regulator there is excellent technique effect, its effect include with
Under:
1. use cost is low, and consumption is few.For example, in 1-2g snp, 10-20g sa and potassium dihydrogen phosphate 1.1000-2.5kg
In the case that (low price) is dissolved in water, only apply 50l for every mu.
2. instant effect, noresidue, non-environmental-pollution.Because snp and sa has ease of solubility, its solution can be quickly by root system
Or blade absorbs, effect is obvious.Snp and sa is safe and harmless to people, animal, environmentally safe, is all described as environmentally friendly
Plant growth regulating substance.
3. application technique is simple, is easy to spread.Pass through seed dressing and foliage-spray in sowing stage of wheat and pustulation period
Liquid medicine, can significantly improve the mass of 1000 kernel of Semen Tritici aestivi and total product.
4., because snp and sa is to the regulatory function of plant and interaction, the two also obtains on Semen Maydiss other crop
Popularization and application.
Brief description
Fig. 1 is for different composite regulator in embodiment 10 to wheat seed alpha-amylase activity (a) and soluble sugar content
The impact of (b).
Fig. 2 is for different composite regulator in embodiment 10 to wheat flag leaf superoxide dismutase (a) and catalase
The impact of active (b).
Fig. 3 is the impact to wheat flag leaf chlorophyll content spad value for the different composite regulator in embodiment 10.
Fig. 4 is the impact to wheat flag leaf Net Photosynthetic Rate for the different composite regulator in embodiment 10.
Fig. 5 is the impact to wheat flag leaf photosynthetic performance index for the different composite regulator in embodiment 10.
Specific embodiment
Now describe the various exemplary embodiment of the present invention in detail, this detailed description is not considered as the limit to the present invention
System, and it is understood as the more detailed description to certain aspects of the invention, characteristic and embodiment.
It should be understood that heretofore described term is only to be to describe special embodiment, it is not intended to limit this
Bright.In addition, for the numerical range in the present invention it is thus understood that it is also specifically disclosed that this scope upper and lower bound between every
Individual intermediate value.In in the intermediate value in any statement value or stated ranges and any other statement value or in described scope
Between each less scope between value be also included in the present invention.These small range of upper and lower bounds can independently include
Or exclusion in the range of.
Unless otherwise stated, all technology used herein and scientific terminology have the routine in field of the present invention
The identical meanings that technical staff is generally understood that.Although the present invention only describes preferred application and composition (for example, regulator group
Become), but can also use and similar or equivalent any application described herein and composition in the enforcement in the present invention or test.
The all documents mentioned in this specification are incorporated by reference into, in order to the disclosure and description application related to described document and/or
Composition.When conflicting with any document being incorporated to, it is defined by the content of this specification.
In the present invention, vocabulary of terms had both included singulative, also included plural form, unless context separately clearly refers to
Go out.Heretofore described " at least one " or " at least one " refer not only to the situation that comprises " one " or " a kind of ", heavier
The situation also comprising " multiple " or " multiple " wanted.
Experimental technique used in the present invention if no special instructions, is conventional method.Material used, reagent etc. as
No specified otherwise, all commercially obtains.
In the present invention, described term " composite regulator " is by sodium nitroprusside, salicylic acid and three kinds of compositions of potassium dihydrogen phosphate
The aqueous solution of composition.It should be noted that inevitably comprising in the composite regulator of the present invention with Impure forms presence
A small amount of other compositions.Particulate matter, other ionic species can be illustrated as other compositions (except sodium nitroprusside, salicylic acid and di(2-ethylhexyl)phosphate
Ion outside the ion that hydrogen potassium three produces), colloid etc..
As particulate matter, it includes insoluble 10 μm to 50 μm in water of inorganic and organic impuritiess, its normal conditions
Under be that naked eyes are sightless, but sometimes also can comprise more than 50 μm of granules according to different situations, now granule is that naked eyes are distinguishable
's.The instantiation of particulate matter includes, but are not limited to silt, metal-oxide or hydroxide etc..Under normal circumstances, granule
Material with dissolve or suspended state exist.
As other ionic species, including being dissolved in the material being dissociated into anions and canons after water.Heretofore described " its
His ionic species " refer to the ion in addition to the ion that sodium nitroprusside, salicylic acid and potassium dihydrogen phosphate three produce, and it is included for example
Iron ion, manganese ion.Preferably, the content of these ions is low as far as possible, and preferably 0.If the content of other ionic species
More than 0.3mg/l, then can affect the realization of regulator effect of the present invention, be unfavorable for slow down aging and/or improve yield.Therefore,
The content of other ionic species is necessarily less than 0.3mg/l.
In addition, the material as impurity also includes being dissolved in the gas of water, such as o2、co2、nh3、n2Deng.These gases are in water
Some acid ions, the quality of impact composite regulator is generated after middle reaction.Accordingly, it would be desirable to the content of these gases is as far as possible
Low, preferably 0.
Used in the present invention, " water " can be any type of fresh water, including river, lake water, well water, subsoil water, surface water
Etc. naturally occurring water;Also include the water of artificial treatment, including purified water, mineral water, tap water etc..It should be noted that
In the case of tap water, the disinfectant composition such as sodium hypochlorite in water, may be left.It is therefore preferable that using purified water.
The composite regulator of the present invention can provide in any form, include, but are not limited to bottled, packed or other are any close
Envelope or open state provide.Preferably, the composite regulator of the present invention is provided with the sealing state of lucifuge.
In the present invention, the content of the described sodium nitroprusside weight based on every kg composite regulator is 20-30mg, preferably 22-
28mg, more preferably 24-26mg, most preferably 25mg.The salicylic content of the present invention is 200-300mg, preferably 220-280mg,
More preferably 240-260mg, further preferred 245-255mg, most preferably 250mg.The content of the potassium dihydrogen phosphate of the present invention is
22000-35000mg, preferably 23000-34000mg, more preferably 24000-32000mg, further preferred 26000-30000mg,
For example, 28000mg, 29000mg etc..
" Semen Tritici aestivi " of the present invention refers to Semen Tritici aestivi subtribe triticinae plant.In Semen Tritici aestivi subtribe, in addition to Triticum
Also include Aegilops aegilops, Roegneria C. Koch roeogneria, Genus Agropyron elytrigia, Agropyron agropyron,
Dasypyrum haynaldia, Secale secale and Eremopyrum eremopyrum.Specifically, Semen Tritici aestivi bag of the present invention
Include but be not limited to winter wheat, spring wheat etc..
The consumption of regulator of the present invention, in the case of seed dressing, usually every kilogram of wheat seeds use 50-1000ml
Composite regulator, preferably 60-800ml, more preferably 70-500ml, further preferably 80-300ml, most preferably 100ml.After Semen Tritici aestivi is spent
In the case that 15-20 days use, every mu of ground uses composite regulator, preferably 20-80l, more preferably 30-70l described in 10-100l,
Further preferably 10-60l, most preferably 50l.
The composite regulator of the present invention can be administered to any part of plant.Include spraying, spray, pour as method of application
Fill, spray, smear.As site of administration, including plant leaf blade, stem, root etc..Preferably, by being spray applied plant
At least part of top vane surface of thing.
" medicament " in the present invention is the medicament of any form or composition, as long as the composition of medicament itself does not affect the present invention
The effect of described composite regulator.Any medicament or those skilled in the art can be known in the art as medicament itself
Other medicaments that can be obtained based on currently available technology.Any medicament known in the art comprises medicament on the market,
Also include the medicament of national pesticide approved by management, and the known pharmaceutical agents described in document at present.
In the present invention, the assay method of each index is specific as follows:
1st, seed alpha-amylase activity
Be measured using 3,5- dinitrosalicylic acid system (list of references: Li Hesheng. plant physiology and biochemistry experimental principle and
Technology. Beijing: Higher Education Publishing House, 2000,169-172.).
2nd, seed soluble sugar content
(list of references: yang d l, jing r l, chang x p, w is measured using anthrone colorimetry
li.identification of quantitative trait loci and environmental interactions
for accumula tion and remobilization of water-soluble carbohydrates in wheat
(triticum aestivum l.)stems.genetics,2007,176(1):571-584.).
3rd, the mensure of chlorophyll content
The spad value that spad 2502 type chlorophyll meter measures blade is produced using Japan.
4th, the mensure of photosynthetic rate and photosynthetic performance parameter
Using ciras-2 portable light assembly system (ppsystems, Britain) and continuous agitation formula luminoscope (handy
Pea, hansatech, Britain) respectively Net Photosynthetic Rate (pn), photosynthetic performance index are measured (list of references: Zheng Qiuling,
Tan Wei, Ma Ning etc..The impact [j] to kyoto grape leaf photosynthesis and chlorophyll fluorescence under high temperature for the calcium. Scientia Agricultura Sinica,
2010,43(9):1963-1968).
5th, the mensure of superoxide dismutase and catalase activity
The mensure of sod activity presses giannopolitis and ries (giannopolitis g n, reis s
k.superoxide dismutase i.occurrence in higher plants.plant physiology,1977,
Method 59:309-315) is carried out, and the mensure of cat activity presses aebi (aebi h.catalase in vitro.methods in
Enzymology, 1984,105:121-126.) method measure.
6th, the mensure of mass of 1000 kernel and yield
After results, every cell samples 10 plants, investigates indoors and measures wheat seed mass of 1000 kernel.Grain yield is real according to cell
Receive Production rate per mu yield.
Embodiment
Embodiment 1, preparation composite regulator
According to following rate of charges preparation 1kg composite regulator:
Sodium nitroprusside (molecular formula na2[fe(cn)5no]·2h2O): 20mg/kg;
Salicylic acid (molecular formula c7h6o3): 200mg/kg;
Potassium dihydrogen phosphate (molecular formula kh2po4)22000mg/kg;
Above-mentioned each component is mixed, then adds the water of surplus to 1kg, agitated uniformly obtain composite regulator.
Embodiment 2, preparation composite regulator
According to following rate of charges preparation 1kg composite regulator:
Sodium nitroprusside (molecular formula na2[fe(cn)5no]·2h2O): 20mg/kg;
Salicylic acid (molecular formula c7h6o3): 300mg/kg;
Potassium dihydrogen phosphate (molecular formula kh2po4)35000mg/kg;
Above-mentioned each component is mixed and then adds the water of surplus to 1kg, agitated uniformly obtain composite regulator.
Embodiment 3, preparation composite regulator
According to following rate of charges preparation 1kg composite regulator:
Sodium nitroprusside (molecular formula na2[fe(cn)5no]·2h2O): 20mg/kg;
Salicylic acid (molecular formula c7h6o3): 400mg/kg;
Potassium dihydrogen phosphate (molecular formula kh2po4)50000mg/kg;
Above-mentioned each component is mixed and then adds the water of surplus to 1kg, agitated uniformly obtain composite regulator.
Embodiment 4, preparation composite regulator
According to following rate of charges preparation 1kg composite regulator:
Sodium nitroprusside (molecular formula na2[fe(cn)5no]·2h2O): 30mg/kg;
Salicylic acid (molecular formula c7h6o3): 200mg/kg;
Potassium dihydrogen phosphate (molecular formula kh2po4)35000mg/kg;
Above-mentioned each component is mixed and then adds the water of surplus to 1kg, agitated uniformly obtain composite regulator.
Embodiment 5, preparation composite regulator
According to following rate of charges preparation 1kg composite regulator:
Sodium nitroprusside (molecular formula na2[fe(cn)5no]·2h2O): 30mg/kg;
Salicylic acid (molecular formula c7h6o3): 300mg/kg;
Potassium dihydrogen phosphate (molecular formula kh2po4)22000mg/kg;
Above-mentioned each component is mixed and then adds the water of surplus to 1kg, agitated uniformly obtain composite regulator.
Embodiment 6, preparation composite regulator
According to following rate of charges preparation 1kg composite regulator:
Sodium nitroprusside (molecular formula na2[fe(cn)5no]·2h2O): 30mg/kg;
Salicylic acid (molecular formula c7h6o3): 400mg/kg;
Potassium dihydrogen phosphate (molecular formula kh2po4)50000mg/kg;
Above-mentioned each component is mixed and then adds the water of surplus to 1kg, agitated uniformly obtain composite regulator.
Embodiment 7, preparation composite regulator
According to following rate of charges preparation 1kg composite regulator:
Sodium nitroprusside (molecular formula na2[fe(cn)5no]·2h2O): 40mg/kg;
Salicylic acid (molecular formula c7h6o3): 200mg/kg;
Potassium dihydrogen phosphate (molecular formula kh2po4)22000mg/kg;
Above-mentioned each component is mixed and then adds the water of surplus to 1kg, agitated uniformly obtain composite regulator.
Embodiment 8, preparation composite regulator
According to following rate of charges preparation 1kg composite regulator:
Sodium nitroprusside (molecular formula na2[fe(cn)5no]·2h2O): 40mg/kg;
Salicylic acid (molecular formula c7h6o3): 300mg/kg;
Potassium dihydrogen phosphate (molecular formula kh2po4)35000mg/kg;
Above-mentioned each component is mixed and then adds the water of surplus to 1kg, agitated uniformly obtain composite regulator.
Embodiment 9, preparation composite regulator
According to following rate of charges preparation 1kg composite regulator:
Sodium nitroprusside (molecular formula na2[fe(cn)5no]·2h2O): 40mg/kg;
Salicylic acid (molecular formula c7h6o3): 400mg/kg;
Potassium dihydrogen phosphate (molecular formula kh2po4)50000mg/kg;
Above-mentioned each component is mixed and then adds the water of surplus to 1kg, agitated uniformly obtain composite regulator.
Embodiment 10, preparation composite regulator
According to following rate of charges preparation 1kg composite regulator:
Sodium nitroprusside (molecular formula na2[fe(cn)5no]·2h2O): 50mg/kg;
Salicylic acid (molecular formula c7h6o3): 500mg/kg;
Potassium dihydrogen phosphate (molecular formula kh2po4)60000mg/kg;
Above-mentioned each component is mixed and then adds the water of surplus to 1kg, agitated uniformly obtain composite regulator.
Embodiment 11, the field test of composite regulator of embodiment 1-10 preparation and pot experiment
(1) test site
Test in 2013-2016, carry out in science and technology demonstration area of Agricultural University Of He'nan.
(2) reagent agent
The composite regulator of embodiment 1-10 preparation;Ck is to spray equivalent clear water.
(3) wheat breed
For examination wheat breed week wheat 27 (semi-winterness type), provided by the institute of agricultural sciences of Zhoukou City of Henan Province.
(4) test method
A (embodiment 1), b (embodiment 2), c (embodiment 3), d (embodiment 4), e (embodiment 5), f (embodiment 6), g
(embodiment 7), h (embodiment 8), i (embodiment 9), j (embodiment 10) is with equivalent clear water for comparison;Using dressing seed, spray, mix
The processing mode planted+spray, every kg seed composite regulator 100ml during seed dressing, when sprinkling in latter 15-20 days spent by Semen Tritici aestivi, every mu makes
Use composite regulator 50l.Potted plant identical with land for growing field crops process.Potting soil is taken at land for growing field crops topsoil, and mixing is sieved, the high 30cm of basin, directly
Footpath 45cm, every basin dress soil 20kg, each processes 30 basins, and every basin stays 10 plants of Seedling.Field experiment adopts randomized block design, 3 weights
Multiple, plot area 24m2(6m × 4m), every cell application rate 0.6kg, artificial dibbling, field management is with general high-yield field.
(5) application effect
5.1) promote wheat seeds sprouting
The impact to wheat seed germinating rate and germinating energy for each test combinations matched group is as shown in table 1.
The impact to wheat seed germinating rate and germinating energy for the table 1 different composite regulator
Can be learnt by the above results, accelerate the sprouting process of wheat seed using the composite regulator seed dressing of the present invention.
After a, b, c, d, e, f, g, h, i seed dressing percentage of seedgermination be respectively increased 1.9%, 1.0%, 2.6%, 4.6%, 3.4%,
2.4%th, 1.9%, 2.1%, 1.3%;Potentiality of seed has been respectively increased 3.8%, 5.6%, 6.3%, 9.1%, 5.7%,
4.2%th, 3.8%, 0.8%.
The impact to wheat seed alpha-amylase activity and soluble sugar content of each test group and matched group is as shown in Figure 1.
Can be learnt by the above results, all can improve Wheat Species to some extent using after the composite regulator seed dressing invented
The alpha-amylase activity of endosperm in son, promotes the hydrolysis of reserve substance in seed, and sprouting for seed provides more available battalion
Support and be worth.Each process be respectively increased 3.0% compared with clear water comparison, 24.8%, 62.3%, 81.0%, 58.9%, 76.8%%,
41.2%th, 26.2%, 19.5%, soluble sugar content increased 14.6% respectively, 13.8%, 36.4%, 53.1%,
51.8%th, 35.0%, 1.2%, 14.0%, 11.2%.
5.2) improve the activity of wheat flag leaf antioxidase
The impact to wheat flag leaf activities of antioxidant enzymes of each test group and matched group is as shown in Figure 2.
Can be learnt by the above results, after spraying different composite regulator after spending, significantly increase pustulation period wheat flag leaf antioxygen
Change enzymatic activity it is suppressed that peroxidation of membrane lipids, prevent leaf presenility.Sod, cat enzymatic activity that composite regulator d group is processed is relatively
Clear water compares raising 33.3%, 42.7%.
5.3) improve the photosynthetic capacity of wheat flag leaf
The impact to wheat flag leaf photosynthetic capacity of each test group and matched group is as in Figure 3-5.
Can be learnt by the above results, increase wheat flag leaf chlorophyll content after spraying different composite regulator after spending, extend
Boot leaf functional period, improves its photosynthetic rate and photosynthetic capacity.The chlorophyll content of composite regulator d group process, Net Photosynthetic Rate
Increased 17.5%, 23.0%, 36.6% with photosynthetic performance index compared with matched group respectively.
5.4) wheat seed mass of 1000 kernel and total product are improved
The impact to wheat flag leaf photosynthetic capacity of each test group and matched group is as shown in table 2.
The impact to wheat seed mass of 1000 kernel and yield of table 2 composite regulator and processing mode
As can be seen from Table 2, composite regulator of the present invention all can improve wheat seed mass of 1000 kernel and yield to some extent,
Composite regulator occupation mode is sprayed for optimal with after dress seed+spend, and the mass of 1000 kernel that d group is processed averagely increases 4.05g, and volume increase reaches
6.5%.
Optimal technical scheme with reference to the application describes the application in detail, however, it is necessary to explanation, without departing from this
Application spirit in the case of, those skilled in the art can make on the basis of above disclosure any transformation, modify with
And change.The application includes above-mentioned specific embodiments and its any equivalents.
Claims (9)
1. a kind of composite regulator, the composition of every kilogram of composite regulator is as follows:
Sodium nitroprusside 20~40mg;
Salicylic acid 200~400mg;
Potassium dihydrogen phosphate 22000~50000mg;With
The water of surplus.
2. composite regulator according to claim 1, the composition of every kilogram of composite regulator is selected from 1) to 8) shown in composition
In any one:
1) sodium nitroprusside 20~30mg, the water of salicylic acid 200~300mg, potassium dihydrogen phosphate 22000~35000mg and surplus;
2) sodium nitroprusside 30~40mg, the water of salicylic acid 200~400mg, potassium dihydrogen phosphate 35000~50000mg and surplus;
3) sodium nitroprusside 20mg, the water of salicylic acid 400mg, potassium dihydrogen phosphate 50000mg and surplus;
4) sodium nitroprusside 30mg, the water of salicylic acid 400mg, potassium dihydrogen phosphate 50000mg and surplus;
5) sodium nitroprusside 40mg, the water of salicylic acid 200~300mg, potassium dihydrogen phosphate 35000~50000mg and surplus;
6) sodium nitroprusside 30~40mg, the water of salicylic acid 200~300mg, potassium dihydrogen phosphate 35000~50000mg and surplus;
7) sodium nitroprusside 20~30mg, the water of salicylic acid 300~400mg, potassium dihydrogen phosphate 35000~50000mg and surplus;
8) sodium nitroprusside 30~40mg, the water of salicylic acid 300~400mg, potassium dihydrogen phosphate 35000~50000mg and surplus.
3. application in adjusting wheat growth for the composite regulator according to claim 1 and 2.
4. according to claim 3 application it is characterised in that: described composite regulator is used for delaying Wheat Leaves Senescence
And/or raising wheat yield.
5. according to claim 3 application it is characterised in that: described application be selected from least one of following:
(1) improve wheat leaf blade chlorophyll content
(2) improve wheat leaf blade photosynthetic rate
(3) improve Photosynthetic Function of Wheat Leaf index
(4) increase wheat leaf blade superoxide dismutase activity
(5) increase wheat leaf blade catalase activity
(6) improve wheat seed mass of 1000 kernel
(7) increase wheat yield.
6. the application according to any one of claim 3-5 it is characterised in that: make according to selected from any one of following manner
Use described composite regulator:
(1) mode dressed seed;
(2) spray the mode of Semen Tritici aestivi;
(3) mode that (1) and (2) combines.
7. the application according to any one of claim 3-5 it is characterised in that: the consumption of described composite regulator is:
Every kilogram of wheat seed is dressed seed using composite regulator described in 100ml, and/or 15-20 days after Semen Tritici aestivi is spent, every mu
Ground is uniformly sprayed to upper blade using composite regulator described in 10-100l.
8. a kind of medicament delaying Wheat Leaves Senescence and/or improving wheat yield, it comprises according to claim 1 or claim 2
Composite regulator.
9. a kind of method delaying Wheat Leaves Senescence and/or improving wheat yield, wherein uses according to claim 1 or 2 institute
The composite regulator stated.
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