CN106359451A - Compound conditioning agent for delaying senility of leaves of wheat and/or improving yield, medicament comprising same, and application of same - Google Patents

Compound conditioning agent for delaying senility of leaves of wheat and/or improving yield, medicament comprising same, and application of same Download PDF

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Publication number
CN106359451A
CN106359451A CN201610727534.3A CN201610727534A CN106359451A CN 106359451 A CN106359451 A CN 106359451A CN 201610727534 A CN201610727534 A CN 201610727534A CN 106359451 A CN106359451 A CN 106359451A
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wheat
composite regulator
water
salicylic acid
surplus
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CN106359451B (en
Inventor
邵瑞鑫
康国章
黄勇
杨青华
郭家萌
贾琳
郑会芳
毛俊
韩心培
郑博元
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Henan Agricultural University
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Henan Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/26Phosphorus; Compounds thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A01N37/38Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
    • A01N37/40Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/24Cyanogen or compounds thereof, e.g. hydrogen cyanide, cyanic acid, cyanamide, thiocyanic acid

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Toxicology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention provides a compound conditioning agent for delaying senility of leaves of wheat and/or improving yield, an application of same and a medicament comprising same, wherein 1 kg of the compound conditioning agent includes: 20-40 mg of sodium nitroprusside, 200-400 mg of salicylic acid, 22000-50000 mg of monopotassium phosphate, and the balanced being water. The compound conditioning agent can delay the senility of leaves of wheat and/or improve the yield of wheat, and has excellent technical effects.

Description

A kind of delay Wheat Leaves Senescence and/or improve the composite regulator of yield, medicament and Its application
Technical field
The present invention relates to a kind of composite regulator delaying Wheat Leaves Senescence and/or improving wheat yield, belong to plant Regulator field.
Background technology
Huang-Huai-Hai is the most important producing region of China's Semen Tritici aestivi, and this area's Growth of Wheat has tillering stage length, young fringe divides Change phase length and the feature of pustulation period short (" two long one is short "), thus this area's wheat tillering disclosure satisfy that mature spike number demand substantially, and Spend more many with the obvious advantage, advantageously form many of big fringe, but due to Huang-Huai-Hai Wheat Plant in Late Filling Period often occur unfavorable Weather, especially high temperature, arid, leaf chlorophyll enzyme, Lipid peroxidation and protective enzyme system superoxide dismutase (sod), catalase (cat), peroxidase and ascorbic acid oxidase isoreactivity change, and cause leaf photosynthesis energy Power declines and leaf senile.Due to pustulation period seed starch synthesis mainly with blade synthesis photosynthate as raw material, because If this pustulation period meets adverse environmental factor, in many wheat breed seeds, the dynamic accumulation of starch is restricted, and causes these kinds high Grain weight potentiality are difficult to be not fully exerted, thus constrain the raising further of yield.
The mass of 1000 kernel potentiality of Huang-Huai-Hai spread wheat breed are mostly in more than 48g now, but actual production In, throughout the year in below 42g, plumpness is poor for the mass of 1000 kernel of these kinds, therefore, only this index of grain weight, these kinds increase Produce potentiality up to 60kg/667m2(test summarized materials according to Winter Wheat In Henan Province kind region 2008-2015, with thousand above Grain weight often increases 1g, every 667m2Increase 10kg to calculate).Therefore, improving Grain Weight in Common Wheat is the important channel improving yield.
Nitric oxide (nitric oxide, no) is a kind of bioactive molecule and the plant life of latest find in plant body Long Auto-regulator (cevahir etc., 2003).When by environment stress, Plant emission goes out no and participates in photosynthetic regulation and control, Thus improving resistance become one of hot issue of domestic and international plant stress physiology research.Phytohormone is to growth metabolism Regulation be complicated, also generally existing (the pospisilova et al.2005) of the mutual effect and between other Auto-regulator. Research shows no and other phytohormone such as salicylic acid (sa), abscisic acid (aba), jasmonic (ja), h2o2There is mutually collaborative work With, improve activities of antioxidant enzymes reduce active oxygen produce thus protect Photosynthetic from stress injury (Zhang Yikai etc., 2010).
Some plants all can be significantly improved with regard to this two classes material of no and sa and its functional analogue to biotic and non- The resistance of biotic, increases crop yield, and low production cost, nontoxic, noresidue, shows huge in production practices Application potential.But delay Wheat Leaves Senescence about this two classes Auto-regulator, there is not been reported for the research of raising wheat yield.For This, this experimental study the two improve the effect of wheat yield, delay Wheat Leaves Senescence and improve Semen Tritici aestivi thousand to develop Weight and the composite regulator of yield.
Content of the invention
The technical problem to be solved is to provide one kind to delay Wheat Leaves Senescence for the deficiencies in the prior art And/or improve the composite regulator of wheat yield.
An aspect of of the present present invention, provides a kind of composite regulator, and the composition of every kilogram of described composite regulator is as follows: nitre is general Sodium (snp) 20~40mg;Salicylic acid 200~400mg;Potassium dihydrogen phosphate 22000~50000mg;Water with surplus.
In a preferred embodiment, the composite regulator of the present invention, based on composite regulator every kilogram described, its composition Any one of it is preferably following 1)~8):
1) sodium nitroprusside 20~30mg;Salicylic acid 200~300mg;Potassium dihydrogen phosphate 22000~35000mg;With surplus Water;
2) sodium nitroprusside 30~40mg;Salicylic acid 200~400mg;Potassium dihydrogen phosphate 35000~50000mg;With surplus Water;
3) sodium nitroprusside 20mg;Salicylic acid 400mg;Potassium dihydrogen phosphate 50000mg;Water with surplus;
4) sodium nitroprusside 30mg;Salicylic acid 400mg;Potassium dihydrogen phosphate 50000mg;Water with surplus;
5) sodium nitroprusside 40mg;Salicylic acid 200~300mg;Potassium dihydrogen phosphate 35000~50000mg;Water with surplus;
6) sodium nitroprusside 30~40mg;Salicylic acid 200~300mg;Potassium dihydrogen phosphate 35000~50000mg;With surplus Water;
7) sodium nitroprusside 20~30mg;Salicylic acid 300~400mg;Potassium dihydrogen phosphate 35000~50000mg;With surplus Water;
8) sodium nitroprusside 30~40mg;Salicylic acid 300~400mg;Potassium dihydrogen phosphate 35000~50000mg;With surplus Water.
Another aspect of the present invention, also provides application in adjusting wheat growth for the described composite regulator.Preferred In embodiment, can be used for delaying Wheat Leaves Senescence and/or improve wheat yield.For example, be selected from following any one In application:
(1) improve wheat leaf blade chlorophyll content
(2) improve wheat leaf blade photosynthetic rate
(3) improve Photosynthetic Function of Wheat Leaf index
(4) increase wheat leaf blade superoxide dismutase activity
(5) increase wheat leaf blade catalase activity
(6) improve wheat seed mass of 1000 kernel
(7) increase wheat yield.
In a preferred embodiment, preferably in the following manner using described compound in the above-mentioned application of the present invention Regulator:
Use described composite regulator by way of seed dressing or in the mode that Semen Tritici aestivi spends spray for latter 15-20 days, more excellent The mode that both choosings are used in combination.
Another aspect of the invention, provides a kind of medicament delaying Wheat Leaves Senescence and/or improving wheat yield, its bag Containing composite regulator of the present invention.
Another aspect of the present invention, provides a kind of method delaying Wheat Leaves Senescence and/or improving wheat yield, wherein Using composite regulator of the present invention.
Show through the test of pesticide effectiveness, the present invention provide composite regulator there is excellent technique effect, its effect include with Under:
1. use cost is low, and consumption is few.For example, in 1-2g snp, 10-20g sa and potassium dihydrogen phosphate 1.1000-2.5kg In the case that (low price) is dissolved in water, only apply 50l for every mu.
2. instant effect, noresidue, non-environmental-pollution.Because snp and sa has ease of solubility, its solution can be quickly by root system Or blade absorbs, effect is obvious.Snp and sa is safe and harmless to people, animal, environmentally safe, is all described as environmentally friendly Plant growth regulating substance.
3. application technique is simple, is easy to spread.Pass through seed dressing and foliage-spray in sowing stage of wheat and pustulation period Liquid medicine, can significantly improve the mass of 1000 kernel of Semen Tritici aestivi and total product.
4., because snp and sa is to the regulatory function of plant and interaction, the two also obtains on Semen Maydiss other crop Popularization and application.
Brief description
Fig. 1 is for different composite regulator in embodiment 10 to wheat seed alpha-amylase activity (a) and soluble sugar content The impact of (b).
Fig. 2 is for different composite regulator in embodiment 10 to wheat flag leaf superoxide dismutase (a) and catalase The impact of active (b).
Fig. 3 is the impact to wheat flag leaf chlorophyll content spad value for the different composite regulator in embodiment 10.
Fig. 4 is the impact to wheat flag leaf Net Photosynthetic Rate for the different composite regulator in embodiment 10.
Fig. 5 is the impact to wheat flag leaf photosynthetic performance index for the different composite regulator in embodiment 10.
Specific embodiment
Now describe the various exemplary embodiment of the present invention in detail, this detailed description is not considered as the limit to the present invention System, and it is understood as the more detailed description to certain aspects of the invention, characteristic and embodiment.
It should be understood that heretofore described term is only to be to describe special embodiment, it is not intended to limit this Bright.In addition, for the numerical range in the present invention it is thus understood that it is also specifically disclosed that this scope upper and lower bound between every Individual intermediate value.In in the intermediate value in any statement value or stated ranges and any other statement value or in described scope Between each less scope between value be also included in the present invention.These small range of upper and lower bounds can independently include Or exclusion in the range of.
Unless otherwise stated, all technology used herein and scientific terminology have the routine in field of the present invention The identical meanings that technical staff is generally understood that.Although the present invention only describes preferred application and composition (for example, regulator group Become), but can also use and similar or equivalent any application described herein and composition in the enforcement in the present invention or test. The all documents mentioned in this specification are incorporated by reference into, in order to the disclosure and description application related to described document and/or Composition.When conflicting with any document being incorporated to, it is defined by the content of this specification.
In the present invention, vocabulary of terms had both included singulative, also included plural form, unless context separately clearly refers to Go out.Heretofore described " at least one " or " at least one " refer not only to the situation that comprises " one " or " a kind of ", heavier The situation also comprising " multiple " or " multiple " wanted.
Experimental technique used in the present invention if no special instructions, is conventional method.Material used, reagent etc. as No specified otherwise, all commercially obtains.
In the present invention, described term " composite regulator " is by sodium nitroprusside, salicylic acid and three kinds of compositions of potassium dihydrogen phosphate The aqueous solution of composition.It should be noted that inevitably comprising in the composite regulator of the present invention with Impure forms presence A small amount of other compositions.Particulate matter, other ionic species can be illustrated as other compositions (except sodium nitroprusside, salicylic acid and di(2-ethylhexyl)phosphate Ion outside the ion that hydrogen potassium three produces), colloid etc..
As particulate matter, it includes insoluble 10 μm to 50 μm in water of inorganic and organic impuritiess, its normal conditions Under be that naked eyes are sightless, but sometimes also can comprise more than 50 μm of granules according to different situations, now granule is that naked eyes are distinguishable 's.The instantiation of particulate matter includes, but are not limited to silt, metal-oxide or hydroxide etc..Under normal circumstances, granule Material with dissolve or suspended state exist.
As other ionic species, including being dissolved in the material being dissociated into anions and canons after water.Heretofore described " its His ionic species " refer to the ion in addition to the ion that sodium nitroprusside, salicylic acid and potassium dihydrogen phosphate three produce, and it is included for example Iron ion, manganese ion.Preferably, the content of these ions is low as far as possible, and preferably 0.If the content of other ionic species More than 0.3mg/l, then can affect the realization of regulator effect of the present invention, be unfavorable for slow down aging and/or improve yield.Therefore, The content of other ionic species is necessarily less than 0.3mg/l.
In addition, the material as impurity also includes being dissolved in the gas of water, such as o2、co2、nh3、n2Deng.These gases are in water Some acid ions, the quality of impact composite regulator is generated after middle reaction.Accordingly, it would be desirable to the content of these gases is as far as possible Low, preferably 0.
Used in the present invention, " water " can be any type of fresh water, including river, lake water, well water, subsoil water, surface water Etc. naturally occurring water;Also include the water of artificial treatment, including purified water, mineral water, tap water etc..It should be noted that In the case of tap water, the disinfectant composition such as sodium hypochlorite in water, may be left.It is therefore preferable that using purified water.
The composite regulator of the present invention can provide in any form, include, but are not limited to bottled, packed or other are any close Envelope or open state provide.Preferably, the composite regulator of the present invention is provided with the sealing state of lucifuge.
In the present invention, the content of the described sodium nitroprusside weight based on every kg composite regulator is 20-30mg, preferably 22- 28mg, more preferably 24-26mg, most preferably 25mg.The salicylic content of the present invention is 200-300mg, preferably 220-280mg, More preferably 240-260mg, further preferred 245-255mg, most preferably 250mg.The content of the potassium dihydrogen phosphate of the present invention is 22000-35000mg, preferably 23000-34000mg, more preferably 24000-32000mg, further preferred 26000-30000mg, For example, 28000mg, 29000mg etc..
" Semen Tritici aestivi " of the present invention refers to Semen Tritici aestivi subtribe triticinae plant.In Semen Tritici aestivi subtribe, in addition to Triticum Also include Aegilops aegilops, Roegneria C. Koch roeogneria, Genus Agropyron elytrigia, Agropyron agropyron, Dasypyrum haynaldia, Secale secale and Eremopyrum eremopyrum.Specifically, Semen Tritici aestivi bag of the present invention Include but be not limited to winter wheat, spring wheat etc..
The consumption of regulator of the present invention, in the case of seed dressing, usually every kilogram of wheat seeds use 50-1000ml Composite regulator, preferably 60-800ml, more preferably 70-500ml, further preferably 80-300ml, most preferably 100ml.After Semen Tritici aestivi is spent In the case that 15-20 days use, every mu of ground uses composite regulator, preferably 20-80l, more preferably 30-70l described in 10-100l, Further preferably 10-60l, most preferably 50l.
The composite regulator of the present invention can be administered to any part of plant.Include spraying, spray, pour as method of application Fill, spray, smear.As site of administration, including plant leaf blade, stem, root etc..Preferably, by being spray applied plant At least part of top vane surface of thing.
" medicament " in the present invention is the medicament of any form or composition, as long as the composition of medicament itself does not affect the present invention The effect of described composite regulator.Any medicament or those skilled in the art can be known in the art as medicament itself Other medicaments that can be obtained based on currently available technology.Any medicament known in the art comprises medicament on the market, Also include the medicament of national pesticide approved by management, and the known pharmaceutical agents described in document at present.
In the present invention, the assay method of each index is specific as follows:
1st, seed alpha-amylase activity
Be measured using 3,5- dinitrosalicylic acid system (list of references: Li Hesheng. plant physiology and biochemistry experimental principle and Technology. Beijing: Higher Education Publishing House, 2000,169-172.).
2nd, seed soluble sugar content
(list of references: yang d l, jing r l, chang x p, w is measured using anthrone colorimetry li.identification of quantitative trait loci and environmental interactions for accumula tion and remobilization of water-soluble carbohydrates in wheat (triticum aestivum l.)stems.genetics,2007,176(1):571-584.).
3rd, the mensure of chlorophyll content
The spad value that spad 2502 type chlorophyll meter measures blade is produced using Japan.
4th, the mensure of photosynthetic rate and photosynthetic performance parameter
Using ciras-2 portable light assembly system (ppsystems, Britain) and continuous agitation formula luminoscope (handy Pea, hansatech, Britain) respectively Net Photosynthetic Rate (pn), photosynthetic performance index are measured (list of references: Zheng Qiuling, Tan Wei, Ma Ning etc..The impact [j] to kyoto grape leaf photosynthesis and chlorophyll fluorescence under high temperature for the calcium. Scientia Agricultura Sinica, 2010,43(9):1963-1968).
5th, the mensure of superoxide dismutase and catalase activity
The mensure of sod activity presses giannopolitis and ries (giannopolitis g n, reis s k.superoxide dismutase i.occurrence in higher plants.plant physiology,1977, Method 59:309-315) is carried out, and the mensure of cat activity presses aebi (aebi h.catalase in vitro.methods in Enzymology, 1984,105:121-126.) method measure.
6th, the mensure of mass of 1000 kernel and yield
After results, every cell samples 10 plants, investigates indoors and measures wheat seed mass of 1000 kernel.Grain yield is real according to cell Receive Production rate per mu yield.
Embodiment
Embodiment 1, preparation composite regulator
According to following rate of charges preparation 1kg composite regulator:
Sodium nitroprusside (molecular formula na2[fe(cn)5no]·2h2O): 20mg/kg;
Salicylic acid (molecular formula c7h6o3): 200mg/kg;
Potassium dihydrogen phosphate (molecular formula kh2po4)22000mg/kg;
Above-mentioned each component is mixed, then adds the water of surplus to 1kg, agitated uniformly obtain composite regulator.
Embodiment 2, preparation composite regulator
According to following rate of charges preparation 1kg composite regulator:
Sodium nitroprusside (molecular formula na2[fe(cn)5no]·2h2O): 20mg/kg;
Salicylic acid (molecular formula c7h6o3): 300mg/kg;
Potassium dihydrogen phosphate (molecular formula kh2po4)35000mg/kg;
Above-mentioned each component is mixed and then adds the water of surplus to 1kg, agitated uniformly obtain composite regulator.
Embodiment 3, preparation composite regulator
According to following rate of charges preparation 1kg composite regulator:
Sodium nitroprusside (molecular formula na2[fe(cn)5no]·2h2O): 20mg/kg;
Salicylic acid (molecular formula c7h6o3): 400mg/kg;
Potassium dihydrogen phosphate (molecular formula kh2po4)50000mg/kg;
Above-mentioned each component is mixed and then adds the water of surplus to 1kg, agitated uniformly obtain composite regulator.
Embodiment 4, preparation composite regulator
According to following rate of charges preparation 1kg composite regulator:
Sodium nitroprusside (molecular formula na2[fe(cn)5no]·2h2O): 30mg/kg;
Salicylic acid (molecular formula c7h6o3): 200mg/kg;
Potassium dihydrogen phosphate (molecular formula kh2po4)35000mg/kg;
Above-mentioned each component is mixed and then adds the water of surplus to 1kg, agitated uniformly obtain composite regulator.
Embodiment 5, preparation composite regulator
According to following rate of charges preparation 1kg composite regulator:
Sodium nitroprusside (molecular formula na2[fe(cn)5no]·2h2O): 30mg/kg;
Salicylic acid (molecular formula c7h6o3): 300mg/kg;
Potassium dihydrogen phosphate (molecular formula kh2po4)22000mg/kg;
Above-mentioned each component is mixed and then adds the water of surplus to 1kg, agitated uniformly obtain composite regulator.
Embodiment 6, preparation composite regulator
According to following rate of charges preparation 1kg composite regulator:
Sodium nitroprusside (molecular formula na2[fe(cn)5no]·2h2O): 30mg/kg;
Salicylic acid (molecular formula c7h6o3): 400mg/kg;
Potassium dihydrogen phosphate (molecular formula kh2po4)50000mg/kg;
Above-mentioned each component is mixed and then adds the water of surplus to 1kg, agitated uniformly obtain composite regulator.
Embodiment 7, preparation composite regulator
According to following rate of charges preparation 1kg composite regulator:
Sodium nitroprusside (molecular formula na2[fe(cn)5no]·2h2O): 40mg/kg;
Salicylic acid (molecular formula c7h6o3): 200mg/kg;
Potassium dihydrogen phosphate (molecular formula kh2po4)22000mg/kg;
Above-mentioned each component is mixed and then adds the water of surplus to 1kg, agitated uniformly obtain composite regulator.
Embodiment 8, preparation composite regulator
According to following rate of charges preparation 1kg composite regulator:
Sodium nitroprusside (molecular formula na2[fe(cn)5no]·2h2O): 40mg/kg;
Salicylic acid (molecular formula c7h6o3): 300mg/kg;
Potassium dihydrogen phosphate (molecular formula kh2po4)35000mg/kg;
Above-mentioned each component is mixed and then adds the water of surplus to 1kg, agitated uniformly obtain composite regulator.
Embodiment 9, preparation composite regulator
According to following rate of charges preparation 1kg composite regulator:
Sodium nitroprusside (molecular formula na2[fe(cn)5no]·2h2O): 40mg/kg;
Salicylic acid (molecular formula c7h6o3): 400mg/kg;
Potassium dihydrogen phosphate (molecular formula kh2po4)50000mg/kg;
Above-mentioned each component is mixed and then adds the water of surplus to 1kg, agitated uniformly obtain composite regulator.
Embodiment 10, preparation composite regulator
According to following rate of charges preparation 1kg composite regulator:
Sodium nitroprusside (molecular formula na2[fe(cn)5no]·2h2O): 50mg/kg;
Salicylic acid (molecular formula c7h6o3): 500mg/kg;
Potassium dihydrogen phosphate (molecular formula kh2po4)60000mg/kg;
Above-mentioned each component is mixed and then adds the water of surplus to 1kg, agitated uniformly obtain composite regulator.
Embodiment 11, the field test of composite regulator of embodiment 1-10 preparation and pot experiment
(1) test site
Test in 2013-2016, carry out in science and technology demonstration area of Agricultural University Of He'nan.
(2) reagent agent
The composite regulator of embodiment 1-10 preparation;Ck is to spray equivalent clear water.
(3) wheat breed
For examination wheat breed week wheat 27 (semi-winterness type), provided by the institute of agricultural sciences of Zhoukou City of Henan Province.
(4) test method
A (embodiment 1), b (embodiment 2), c (embodiment 3), d (embodiment 4), e (embodiment 5), f (embodiment 6), g (embodiment 7), h (embodiment 8), i (embodiment 9), j (embodiment 10) is with equivalent clear water for comparison;Using dressing seed, spray, mix The processing mode planted+spray, every kg seed composite regulator 100ml during seed dressing, when sprinkling in latter 15-20 days spent by Semen Tritici aestivi, every mu makes Use composite regulator 50l.Potted plant identical with land for growing field crops process.Potting soil is taken at land for growing field crops topsoil, and mixing is sieved, the high 30cm of basin, directly Footpath 45cm, every basin dress soil 20kg, each processes 30 basins, and every basin stays 10 plants of Seedling.Field experiment adopts randomized block design, 3 weights Multiple, plot area 24m2(6m × 4m), every cell application rate 0.6kg, artificial dibbling, field management is with general high-yield field.
(5) application effect
5.1) promote wheat seeds sprouting
The impact to wheat seed germinating rate and germinating energy for each test combinations matched group is as shown in table 1.
The impact to wheat seed germinating rate and germinating energy for the table 1 different composite regulator
Can be learnt by the above results, accelerate the sprouting process of wheat seed using the composite regulator seed dressing of the present invention. After a, b, c, d, e, f, g, h, i seed dressing percentage of seedgermination be respectively increased 1.9%, 1.0%, 2.6%, 4.6%, 3.4%, 2.4%th, 1.9%, 2.1%, 1.3%;Potentiality of seed has been respectively increased 3.8%, 5.6%, 6.3%, 9.1%, 5.7%, 4.2%th, 3.8%, 0.8%.
The impact to wheat seed alpha-amylase activity and soluble sugar content of each test group and matched group is as shown in Figure 1.
Can be learnt by the above results, all can improve Wheat Species to some extent using after the composite regulator seed dressing invented The alpha-amylase activity of endosperm in son, promotes the hydrolysis of reserve substance in seed, and sprouting for seed provides more available battalion Support and be worth.Each process be respectively increased 3.0% compared with clear water comparison, 24.8%, 62.3%, 81.0%, 58.9%, 76.8%%, 41.2%th, 26.2%, 19.5%, soluble sugar content increased 14.6% respectively, 13.8%, 36.4%, 53.1%, 51.8%th, 35.0%, 1.2%, 14.0%, 11.2%.
5.2) improve the activity of wheat flag leaf antioxidase
The impact to wheat flag leaf activities of antioxidant enzymes of each test group and matched group is as shown in Figure 2.
Can be learnt by the above results, after spraying different composite regulator after spending, significantly increase pustulation period wheat flag leaf antioxygen Change enzymatic activity it is suppressed that peroxidation of membrane lipids, prevent leaf presenility.Sod, cat enzymatic activity that composite regulator d group is processed is relatively Clear water compares raising 33.3%, 42.7%.
5.3) improve the photosynthetic capacity of wheat flag leaf
The impact to wheat flag leaf photosynthetic capacity of each test group and matched group is as in Figure 3-5.
Can be learnt by the above results, increase wheat flag leaf chlorophyll content after spraying different composite regulator after spending, extend Boot leaf functional period, improves its photosynthetic rate and photosynthetic capacity.The chlorophyll content of composite regulator d group process, Net Photosynthetic Rate Increased 17.5%, 23.0%, 36.6% with photosynthetic performance index compared with matched group respectively.
5.4) wheat seed mass of 1000 kernel and total product are improved
The impact to wheat flag leaf photosynthetic capacity of each test group and matched group is as shown in table 2.
The impact to wheat seed mass of 1000 kernel and yield of table 2 composite regulator and processing mode
As can be seen from Table 2, composite regulator of the present invention all can improve wheat seed mass of 1000 kernel and yield to some extent, Composite regulator occupation mode is sprayed for optimal with after dress seed+spend, and the mass of 1000 kernel that d group is processed averagely increases 4.05g, and volume increase reaches 6.5%.
Optimal technical scheme with reference to the application describes the application in detail, however, it is necessary to explanation, without departing from this Application spirit in the case of, those skilled in the art can make on the basis of above disclosure any transformation, modify with And change.The application includes above-mentioned specific embodiments and its any equivalents.

Claims (9)

1. a kind of composite regulator, the composition of every kilogram of composite regulator is as follows:
Sodium nitroprusside 20~40mg;
Salicylic acid 200~400mg;
Potassium dihydrogen phosphate 22000~50000mg;With
The water of surplus.
2. composite regulator according to claim 1, the composition of every kilogram of composite regulator is selected from 1) to 8) shown in composition In any one:
1) sodium nitroprusside 20~30mg, the water of salicylic acid 200~300mg, potassium dihydrogen phosphate 22000~35000mg and surplus;
2) sodium nitroprusside 30~40mg, the water of salicylic acid 200~400mg, potassium dihydrogen phosphate 35000~50000mg and surplus;
3) sodium nitroprusside 20mg, the water of salicylic acid 400mg, potassium dihydrogen phosphate 50000mg and surplus;
4) sodium nitroprusside 30mg, the water of salicylic acid 400mg, potassium dihydrogen phosphate 50000mg and surplus;
5) sodium nitroprusside 40mg, the water of salicylic acid 200~300mg, potassium dihydrogen phosphate 35000~50000mg and surplus;
6) sodium nitroprusside 30~40mg, the water of salicylic acid 200~300mg, potassium dihydrogen phosphate 35000~50000mg and surplus;
7) sodium nitroprusside 20~30mg, the water of salicylic acid 300~400mg, potassium dihydrogen phosphate 35000~50000mg and surplus;
8) sodium nitroprusside 30~40mg, the water of salicylic acid 300~400mg, potassium dihydrogen phosphate 35000~50000mg and surplus.
3. application in adjusting wheat growth for the composite regulator according to claim 1 and 2.
4. according to claim 3 application it is characterised in that: described composite regulator is used for delaying Wheat Leaves Senescence And/or raising wheat yield.
5. according to claim 3 application it is characterised in that: described application be selected from least one of following:
(1) improve wheat leaf blade chlorophyll content
(2) improve wheat leaf blade photosynthetic rate
(3) improve Photosynthetic Function of Wheat Leaf index
(4) increase wheat leaf blade superoxide dismutase activity
(5) increase wheat leaf blade catalase activity
(6) improve wheat seed mass of 1000 kernel
(7) increase wheat yield.
6. the application according to any one of claim 3-5 it is characterised in that: make according to selected from any one of following manner Use described composite regulator:
(1) mode dressed seed;
(2) spray the mode of Semen Tritici aestivi;
(3) mode that (1) and (2) combines.
7. the application according to any one of claim 3-5 it is characterised in that: the consumption of described composite regulator is:
Every kilogram of wheat seed is dressed seed using composite regulator described in 100ml, and/or 15-20 days after Semen Tritici aestivi is spent, every mu Ground is uniformly sprayed to upper blade using composite regulator described in 10-100l.
8. a kind of medicament delaying Wheat Leaves Senescence and/or improving wheat yield, it comprises according to claim 1 or claim 2 Composite regulator.
9. a kind of method delaying Wheat Leaves Senescence and/or improving wheat yield, wherein uses according to claim 1 or 2 institute The composite regulator stated.
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CN112703981A (en) * 2021-01-06 2021-04-27 安徽农业大学 Method for relieving wheat photosynthetic performance loss under high-temperature stress
CN114041463A (en) * 2021-11-09 2022-02-15 昆明学院 Agents and method for reduplicating and reducing damage and improving mutagenesis rate of gynura bicolor chromosome

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