CN106879631A - The inducer of inducing paddy rice blast resisting - Google Patents
The inducer of inducing paddy rice blast resisting Download PDFInfo
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- CN106879631A CN106879631A CN201710256507.7A CN201710256507A CN106879631A CN 106879631 A CN106879631 A CN 106879631A CN 201710256507 A CN201710256507 A CN 201710256507A CN 106879631 A CN106879631 A CN 106879631A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/24—Cyanogen or compounds thereof, e.g. hydrogen cyanide, cyanic acid, cyanamide, thiocyanic acid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/12—Iodine, e.g. iodophors; Compounds thereof
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Abstract
The invention discloses a kind of inducer for luring anti-rice anti-rice blast, the component of the inducer of the rice anti-rice blast includes sodium nitroprussiate, salicylic acid, chitosan oligosaccharide, KI, per 100ml inducers in the parts by weight of each component be：The sodium nitroprussiate 0.75g of mass concentration 99.5%~1.5g, the salicylic acid 3g of mass concentration 99.5%~7.5g, the 1.5~3g of chitosan oligosaccharide of mass concentration 95%, the KI 1.0g of mass concentration 99.9%~1.5g, wherein salicylic acid ethanol dissolve, and other use water dissolves.Inducer of the present invention is sprayed in rice leaf in paddy rice beginning ear period, and after being absorbed by blade, the activity of activation paddy rice activity in vivo material and enzyme, resistance Pyricularia oryzae infects and suppresses Spot expansion, prevention and the generation and harm of reduction rice blast.
Description
Technical field
The present invention relates to a kind of inducer, and in particular to a kind of inducer of inducing paddy rice blast resisting, inducing paddy rice resists
Pyricularia oryzae infects, and prevents and reduce the generation of rice blast.
Background technology
Paddy rice is Chinese topmost cereal crops, and rice is the staple food of Chinese more than 65% population.While China or generation
Rice Production amount and the maximum country of rice consumption figure in boundary, therefore Rice Production is to ensureing Food Security in China and stablizing the world
Rice market all plays an important roll.Rice blast (Rice Blast) is caused by Pyricularia oryzae (Magnaporthe oryzae)
One of the rice disease of most serious, be distributed widely in each rice region in the world, there is generation in various degree Chinese South And North Rice Regions every year,
Hazard area and the extent of injury are larger, serious to hinder rice high yield stable yields.Cloudy weather for several days running, humidity are frequently encountered in growth period duration of rice
Many dew, sultry weather conditions, will pandemic rice blast, disaster is caused in a short time.According to the period and position caused harm
Difference, can be divided into seedling pest, leaf pest, section pest, panicle blast and grain pest.It is general again larger with leaf pest, section pest and panicle blast.At present,
The disease is mainly controlled by chemical prevention and plantation resistant variety, but complexity due to the sick pathogen microspecies heredity and
Pathogenic diversity, disease-resistant varieties often lose resistance after the several years is promoted；Agro-chemicals control is due to long-term use agricultural chemicals
The resistance to the action of a drug is also easy to produce, prevention effect is deteriorated, and pesticide dosage is increased year by year, produces pollution, and persticide residue to increase to bad border
Human body health is adversely affected.Under the environmental condition for being suitable for Growth of Magnaporthe Grisea, the disease can cause paddy rice big
The area underproduction, according to relevant report, Rice Yield Loss Caused of the annual whole world caused by rice blast account for paddy rice total output 10%~
15%, economic loss is up to tens dollars.In the plant disease epidemic time, general underproduction 10%-20% is more serious up to 40%-50%, greatly
Even No kernels or seeds are gathered, as in a year of scarcity during generation.
Plant disease induced resistance refers to the defence system using plant in itself, just can be outer in initial introduced disease
The plant that boundary's factor is excited overall resistance level in itself, can excite crop to produce the material of induced resistance reaction to be then referred to as planting
Thing disease inducer.It is the inevitable outcome after chemical pesticide prosperity and development cause adverse consequences, and it is so that its consumption is small, environment
The good advantage of compatibility, will turn into effective substitute of the chemical pesticide of controlling plant diseases.
Li Li etc.（The such as Li Li, Liu Zhenjiao, Guo Xiaoli beta-aminobutyric acid inducing paddy rice blast resistings and plant defenses enzyme activity
The change Jiangsu's agriculture science of property, 2011,39（2）：185-186）It is research object, result of the test hair using beta-aminobutyric acid
Existing, beta-aminobutyric acid can strengthen the defensive ability/resistance ability of Pyricularia oryzae invasion.Liang Liang etc.（Liang Liang, Hu Xuefang, Liu Weiping, wait .20%
Oligomerization acid iodine paddy rice is fallen Wen Bing field control effect research China agronomy circular, 2013,29（36）：351-354）With 20%
Oligomerization acid iodine is research object, and result of the test finds, 20% oligomerization acid iodine falls Wen Bing to paddy rice has good prevention effect.Contain
It is handsome etc.（Sheng Jiao, Yi Yanmei, Feng Xian wait .1831SP inducing paddy rice blast resisting Study on mechanism Agriculture in Hunan science, 2012
（22）：33-34）It is right to study with the 1831SP that OTAC and neopelex are compounded
As experiment finds that 1831SP is remarkably improved PAL, POX, PPO in plant body, while significantly reducing the CAT in plant body
Activity, so as to strengthen plant self-defense ability, resists the invasion of rice blast.Zhang Wenqing etc.（Zhang Wenqing, Feng Huafeng, Zuo Pingping,
Preparation and the Primary Study hubei agricultural sciences of induction blast resisting Deng chitooligosaccharidequaternary quaternary ammonium salts, 2011,50（5）942-945）
With chitooligosaccharidequaternary quaternary ammonium salt as research object, as a result find, chitooligosaccharidequaternary quaternary ammonium salt sprayed application in rice seedling and can significantly be carried on rice shoot
Resistance of the rice seedling high to rice blast.Wu Lamei etc.（Wu Lamei, Bai Lianyang, golden the morning bell wait inducing paddy rice blast resistings
Root of purple-flowered peucedanum inducer application technical research pre-test biological epidemics science, 2012,35（3）：320-322）Boiled with root of purple-flowered peucedanum aqua, the root of purple-flowered peucedanum
Liquid, root of purple-flowered peucedanum original powder are research object, are as a result found, root of purple-flowered peucedanum aqua, root of purple-flowered peucedanum boil liquid, root of purple-flowered peucedanum original powder lure anti-rice anti-rice blast
Effect, wherein, root of purple-flowered peucedanum aqua is to the Induced resistant effect of rice blast apparently higher than root of purple-flowered peucedanum boil liquid and root of purple-flowered peucedanum original powder.Sun Wanchun etc.（Grandson
Wan Chun, Xue Gaofeng, Zhang Jie, wait influence of the silicon to paddy rice defense key enzyme activity and its relation plants battalion with blast resisting
Support and fertilizer journal, 2009,15（5）1023-1028）With silicate as research object, as a result find, applying silicon can significantly reduce water
The incidence of disease and disease index of rice rice blast, prevention effect is up to 60.59%.Liu Mingda etc.（Liu Mingda, Zhang Wanmin chemistry and lifes
Good rice obtains the field efficacy Agricultural University Of Shenyang journal of inducing paddy rice blast resisting, 2002,33（3）：188-190）With 8% good rice
It is research object to obtain granule, is as a result found, field rice is through chemistry and life --- after good rice must be processed, have to rice blast
Certain resistance.
The inducer of anti-crop disease-resistant is being lured to apply for a patent aspect, some experts have also applied for that Patents are protected.
Du Yu light etc. has applied for " a kind of plant induced resistance agent "（Patent No. ZL99122582.1）, which disclose：It is logical there is provided one kind
The enzymic degradation crossed under microbial fermentation combination certain condition obtains the plant induced resistance agent with bioactive ingredients such as oligosaccharides, and
It is applied to soybean, cotton, tobacco, brinjal plant disease." a kind of inducer is passed in control garden crop soil for the application such as Li Baoju
Application in disease "（Patent No. ZL201010117498.1）, which disclose：With phendioxin, 2,3- thiadiazoles -7- formic acid
Trifluoro ethyl ester lures anti-yellowing melon anti-blight, samping off, the anti-root-rot type epidemic disease of capsicum, the anti-root rot of cyclamen, horse as inducer
The anti-shot hole of bell potato, flat mushroom bacteria resistance brown spot.Zhang Shanxue etc. has applied for a kind of " chitosan oligosaccharide group for anti-rice blast
Compound and application thereof and method "（Patent No. ZL201110333070.5）, which disclose：One kind is used for anti-rice blast
Composition pesticide, chitosan oligosaccharide is included in the composition pesticide, the percentage by weight that chitosan oligosaccharide accounts for the composition pesticide is
0.005%-0.02%.Ruan Songlin etc. has applied for " a kind of plant immune inducer and its application "（Application No.
201510263261.7）, which disclose：The immune inducer percentage, active ingredient includes：0.001%-
0.02% amino-oligosaccharide and the phosphite of 0.01%-0.2%, are applied to the anti-helminthosporium maydis of inducing maize.Ruan Songlin etc. applies
" a kind of application of plant immune inducer in downy mildew of garpe and anthracnose is prevented and treated "（Application No. 201510264420.5）,
Which disclose：The immune inducer percentage, active ingredient includes：The amino-oligosaccharide of 0.001%-0.02%
With the phosphite of 0.01%-0.2%, it is applied to induce grape downy mildew resistance and anthracnose.Ruan Songlin etc. has applied for " plant immune
Application of the inducer in preventing and treating cucumber samping off and damping-off "（Application No. 201610111820.7）, which disclose：
The immune inducer percentage, active ingredient includes：The amino-oligosaccharide of 0.001%-0.025%, 0.01%-0.25%
Phosphate, the phosphite of 0.01%-0.25%, be applied to inducing cucumber resisting damping-off and damping-off.Apply the application such as great waves
" a kind of method that spermine induction treatment improves rice bacterial blight resistance and blast resisting "（Application No. 201510706290.6）,
Which disclose：A kind of method that spermine induction treatment improves rice bacterial blight resistance and blast resisting, the method includes planting
Plant ground preparation, seedling management and spermine induction process step.Peng Shuming etc. has applied for that " a kind of inducer should in agricultural chemicals suspension agent
With technology "（Application No. 201610319376.8）, which disclose：1%- is added in common agriculture chemicals fungicide suspending agent
30% inducer, the inducer is made up of salicylic acid and beta-aminobutyric acid, and the ratio of weight and number of salicylic acid and beta-aminobutyric acid is
1:100-100:1, induction plant produces resistance to various pathogens, reduces pesticide dosage.
The content of the invention
The purpose of the present invention is：A kind of inducer of inducing paddy rice blast resisting is provided, is uniformly sprayed in paddy rice beginning ear period
In rice leaf, the activity of activation paddy rice activity in vivo material and enzyme resists infecting for Pyricularia oryzae, prevents and reduce rice blast
Generation, it is to avoid and mitigate harm of the rice blast to paddy rice.
Technical solution of the invention is：The component of the inducer of the inducing paddy rice wheat blast resisting includes that nitre is general
Sodium, salicylic acid, chitosan oligosaccharide, KI, the parts by weight of each component are in every 100ml inducers：The sodium nitroprussiate of mass concentration 99.5%
0.75g~1.5g, the salicylic acid 3g of mass concentration 99.5%~7.5g, the 1.5~3g of chitosan oligosaccharide of mass concentration 95%, mass concentration
99.9% KI 1.0g~1.5g, wherein salicylic acid ethanol dissolve, and other use water dissolves.
The preparation method of the inducer of above-mentioned inducing paddy rice blast resisting is comprised the following steps：
（1）Following components is taken respectively according to parts by weight：The sodium nitroprussiate 0.75g of mass concentration 99.5%~1.5g, mass concentration 99.5%
Salicylic acid 3g~7.5g, the chitosan oligosaccharide 1.5g of mass concentration 95%~3g, the KI 1.0g of mass concentration 99.9%~1.5g；
（2）Salicylic acid ethanol is dissolved, dissolving ratio is that the salicylic acid of 1g mass concentrations 99.5% 3ml ethanol dissolves；
（3）Sodium nitroprussiate water dissolves, dissolving ratio are dissolved for the sodium nitroprussiate 10ml of 1g mass concentrations 99.5%；
（4）To adding chitosan oligosaccharide in sodium nitroprusside solution, and carry out being sufficiently stirred for dissolving；
（5）To step（4）Mixing liquid in add KI, and be sufficiently stirred for dissolving；
（6）By step（2）The salicylic acid ethanol solution of gained adds step（5）Mixing liquid in, stir evenly mixing；
（7）To step（6）Mixed liquor in add a small amount of running water, stir evenly, and be dissolved to 100ml, obtain the anti-rice blast of inducing paddy rice
The inducer of disease.
The application method of the inducer of above-mentioned inducing paddy rice blast resisting is：In paddy rice beginning ear period, per 667m2Use 100ml
Inducer is uniformly sprayed in rice leaf surface to water 30kg, after stirring evenly, and every being sprayed again once after 7 days, consumption is identical.
Compared with prior art, the present invention has following obvious advantage：
First, the inducer of inducing paddy rice blast resisting of the invention is sprayed in rice leaf in paddy rice beginning ear period, is absorbed by blade,
The activity of activation paddy rice activity in vivo material and some enzymes, is effective against the intrusion of Pyricularia oryzae and the extension of scab, so that in advance
Generation that is anti-and reducing rice blast, mitigates harm of the rice blast to paddy rice, reduces the usage amount of chemical pesticide, and protecting agriculture is ecological
Environment and human body health.
2nd, the salicylic acid and chitosan oligosaccharide in the present invention all have the activity of enzyme and active material, resistance in activation paddy rice body
The intrusion of Pyricularia oryzae and the effect of suppression Spot expansion, superposition synergy is compounded by both, reduces each plant demand, is reduced into
This.
3rd, the sodium nitroprussiate in the present invention is the foreign donor of NO, and it is produced substantial amounts of NO after rice absorbing, NO by with
The iron ion of its soluble recepter guanylate cyclase (guanylatecyclase, GC) is combined, and changes the stereochemical structure of GC
So as to improve its activity, and the increase that intracellular second messenger's ring cytimidine list nucleoside phosphorylase (cGMP) generates is further resulted in,
The cGMP of formation acts on phenylalanine ammonia lyase (PAL) and adjusts salicylic acid again（SA）Accumulation so that inducing paddy rice PAL
With the expression of the disease-resistant related gene such as pathogenesis-related proteins (PR-1), and then the disease-resistant of paddy rice is participated in（Rice blast）Reaction.
4th, on the one hand the KI in the present invention is made by being absorbed by rice leaf after foliage-spray by osmotic adjustment
With reduction pathogen spore germination；On the other hand improve the content of the calcium ion in paddy rice body and promote the signals such as salicylic acid point
The dynamic accumulation of son, accelerates the increase of local cell death and cell leakage, signal is further amplified, and these signals promote again
The activity of relevant enzyme in the metabolic processes such as active oxygen, aldehydes matter is entered, so as to increase paddy disease-resistant（Rice blast）Property.
5th, sprayed in rice leaf with paddy rice beginning ear period with the inducer of inducing paddy rice blast resisting of the invention, wherein
Salicylic acid, chitosan oligosaccharide, sodium nitroprussiate, KI mutually act synergistically, to paddy rice head blight inhibiting rate up to more than 48.01%, have
Preferable economic benefit.
6th, the inducer of rice anti-rice blast of the invention is the new prevention of a class and reduces the pathogenetic product of rice blast
Product, can excite plant immune system, play disease-resistant, diseases prevention purpose；Itself has no bactericidal activity, but it can excite plant to produce
Raw systemic acquired resistance, excites the immunologic mechanism of inside plants, the high yield and high quality potentiality of paddy rice is not fully exerted, and ensures
The production of paddy rice and edible safety, protection human body health.
Specific embodiment
Further illustrate technical solution of the invention with reference to specific embodiment, these embodiments it is not intended that
It is the limitation to technical scheme.
Embodiment 1：The inducer of inducing paddy rice blast resisting is prepared according to following steps
（1）Following components is taken respectively according to parts by weight：The sodium nitroprussiate 0.75g of mass concentration 99.5%, the salicylic acid of mass concentration 99.5%
3.00g, the chitosan oligosaccharide 1.50g of mass concentration 95%, the KI 1.00g of mass concentration 99.9%；
（2）Salicylic acid ethanol is dissolved, dissolving ratio is that the salicylic acid of 1.00g mass concentrations 99.5% 3ml ethanol dissolves；
（3）Sodium nitroprussiate water dissolves, dissolving ratio are dissolved for the sodium nitroprussiate 10ml of 1.00g mass concentrations 99.5%；
（4）To adding chitosan oligosaccharide in sodium nitroprusside solution, and carry out being sufficiently stirred for dissolving；
（5）To step（4）Mixing liquid in add KI, and be sufficiently stirred for dissolving；
（6）By step（2）The salicylic acid ethanol solution of gained adds step（5）Mixing liquid in, stir evenly mixing；
（7）To step（6）Mixed liquor in add a small amount of running water, stir evenly, and be dissolved to 100ml, obtain the anti-rice blast of inducing paddy rice
The inducer of disease.
The application method of the inducer of above-mentioned inducing paddy rice blast resisting is：In paddy rice beginning ear period, per 667m2Use 100ml
Inducer is uniformly sprayed in rice leaf surface to water 30kg, after stirring evenly, and every being sprayed again once after 7 days, consumption is identical.
Tested in Huai'an Agricultural Science and Technology Park within 2016, soil fertility is medium on the upper side, preceding stubble is wheat, experiment
If being processed as：The inducer of the inducing paddy rice blast resisting of the present embodiment 1 sprays treatment and compares（The anti-rice of inducing paddy rice is not sprayed
Seasonal febrile diseases inducer）, three repeat, and plot area is 20m2, rice varieties are Huaihe River rice No. 5, the seedling of May 15 in 2016, June 5
Transplanting, line-spacing 30cm, spacing in the rows 13cm, field rich water quality management, pest control measure are identical；On August 20th, 2016, blade face spray
Apply once, sprayed again once every 7 days.In paddy rice dough stage（On September 27th, 2016）The generation feelings of each treatment cell rice blast of investigation
Condition, 20 caves are investigated per cell, record total spike number, morbidity spike number and the sick level of each cell investigation.According to treatment cell and not dispenser
The disease index of check plot calculates relative inhibition of each treatment to rice blast.Rice blast grade scale is：It is 0 grade, disease-free；
1 grade, the loss less than 5% per fringe（Indivedual branch stalk morbidities）；3 grades, loss 6%-20% (morbidity of 1/3 or so branch stalk) per fringe；5 grades, per fringe
Loss 21%-50% (Miho stems main shaft morbidity, grain half is flat)；7 grades, the morbidity of loss 51%-70% (Miho stems, big portion's shrivelled kernel per fringe)；9
Level, 71%-100% is lost per fringe（Miho stems are fallen ill, and cause dead ears).
Disease index=∑（The sick spike numbers at different levels × number of levels）×100/（Investigate total spike number × 9）
State of an illness inhibiting rate=（Check plot disease index-treatment region disease index）/ check plot disease index
Result of the test shows：The rice blast inhibiting rate for spraying the inducer of the inducing paddy rice blast resisting of the present embodiment 1 reaches
48.01%（See the table below）.
Embodiment 2：The inducer of inducing paddy rice blast resisting is prepared according to following steps
（1）Following components is taken respectively according to parts by weight：The sodium nitroprussiate 1.00g of mass concentration 99.5%, the salicylic acid of mass concentration 99.5%
4.50g, the chitosan oligosaccharide 2.00g of mass concentration 95%, the KI 1.25g of mass concentration 99.9%；
（2）Salicylic acid ethanol is dissolved, dissolving ratio is that the salicylic acid of 1.00g mass concentrations 99.5% 3ml ethanol dissolves；
（3）Sodium nitroprussiate water dissolves, dissolving ratio are dissolved for the sodium nitroprussiate 10ml of 1.00g mass concentrations 99.5%；
（4）To adding chitosan oligosaccharide in sodium nitroprusside solution, and carry out being sufficiently stirred for dissolving；
（5）To step（4）Mixing liquid in add KI, and be sufficiently stirred for dissolving；
（6）By step（2）The salicylic acid ethanol solution of gained adds step（5）Mixing liquid in, stir evenly mixing；
（7）To step（6）Mixed liquor in add a small amount of running water, stir evenly, and be dissolved to 100ml, obtain the anti-rice blast of inducing paddy rice
The inducer of disease.
The application method of the inducer of above-mentioned inducing paddy rice blast resisting is：In paddy rice beginning ear period, per 667m2Use 100ml
Inducer is uniformly sprayed in rice leaf surface to water 30kg, after stirring evenly, and every being sprayed again once after 7 days, consumption is identical.
Tested in Huai'an Agricultural Science and Technology Park within 2016, soil fertility is medium on the upper side, preceding stubble is wheat, experiment sets
It is processed as：The inducer of the inducing paddy rice blast resisting of the present embodiment 2 sprays treatment and compares（The anti-rice blast of inducing paddy rice is not sprayed
Sick inducer）, three repeat, and plot area is 20m2, rice varieties are Huaihe River rice No. 5, the seedling of May 15 in 2016, the machine of June 5
Rice transplanting, line-spacing 30cm, spacing in the rows 13cm, field rich water quality management, pest control measure are identical；On August 20th, 2016, foliage-spray
Once, sprayed again once every 7 days.In paddy rice dough stage（On September 27th, 2016）A situation arises for each treatment cell rice blast of investigation,
20 caves are investigated per cell, total spike number, morbidity spike number and the sick level of each cell investigation is recorded.Compareed with not dispenser according to treatment cell
The disease index of cell calculates relative inhibition of each treatment to rice blast.Rice blast grade scale is：It is 0 grade, disease-free；1 grade,
The loss less than 5% per fringe（Indivedual branch stalk morbidities）；3 grades, loss 6%-20% (morbidity of 1/3 or so branch stalk) per fringe；5 grades, the loss per fringe
21%-50% (Miho stems main shaft is fallen ill, and grain half is flat)；7 grades, the morbidity of loss 51%-70% (Miho stems, big portion's shrivelled kernel per fringe)；9 grades, often
Fringe loses 71%-100%（Miho stems are fallen ill, and cause dead ears).
Disease index=∑（The sick spike numbers at different levels × number of levels）×100/（Investigate total spike number × 9）
State of an illness inhibiting rate=（Check plot disease index-treatment region disease index）/ check plot disease index
Result of the test shows：The rice blast inhibiting rate for spraying the inducer of the inducing paddy rice blast resisting of the present embodiment 2 reaches
55.25%（See the table below）.
Embodiment 3：The inducer of inducing paddy rice blast resisting is prepared according to following steps
（1）Following components is taken respectively according to parts by weight：The sodium nitroprussiate 1.25g of mass concentration 99.5%, the salicylic acid of mass concentration 99.5%
6.00g, the chitosan oligosaccharide 3.00g of mass concentration 95%, the KI 1.00g of mass concentration 99.9%；
（2）Salicylic acid ethanol is dissolved, dissolving ratio is that the salicylic acid of 1.00g mass concentrations 99.5% 3ml ethanol dissolves；
（3）Sodium nitroprussiate water dissolves, dissolving ratio are dissolved for the sodium nitroprussiate 10ml of 1.00g mass concentrations 99.5%；
（4）To adding chitosan oligosaccharide in sodium nitroprusside solution, and carry out being sufficiently stirred for dissolving；
（5）To step（4）Mixing liquid in add KI, and be sufficiently stirred for dissolving；
（6）By step（2）The salicylic acid ethanol solution of gained adds step（5）Mixing liquid in, stir evenly mixing；
（7）To step（6）Mixed liquor in add a small amount of running water, stir evenly, and be dissolved to 100ml, obtain the anti-rice blast of inducing paddy rice
The inducer of disease.
The application method of the inducer of above-mentioned inducing paddy rice blast resisting is：In paddy rice beginning ear period, per 667m2Use 100ml
Inducer is uniformly sprayed in rice leaf surface to water 30kg, after stirring evenly, and every being sprayed again once after 7 days, consumption is identical.
Tested in Huai'an Agricultural Science and Technology Park within 2016, soil fertility is medium on the upper side, preceding stubble is wheat, experiment sets
It is processed as：The inducer of the inducing paddy rice blast resisting of the present embodiment 3 sprays treatment and compares（The anti-rice blast of inducing paddy rice is not sprayed
Sick inducer）, three repeat, and plot area is 20m2, rice varieties are Huaihe River rice No. 5, the seedling of May 15 in 2016, the machine of June 5
Rice transplanting, line-spacing 30cm, spacing in the rows 13cm, field rich water quality management, pest control measure are identical；On August 20th, 2016, foliage-spray
Once, sprayed again once every 7 days.In paddy rice dough stage（On September 27th, 2016）A situation arises for each treatment cell rice blast of investigation,
20 caves are investigated per cell, total spike number, morbidity spike number and the sick level of each cell investigation is recorded.Compareed with not dispenser according to treatment cell
The disease index of cell calculates relative inhibition of each treatment to rice blast.Rice blast grade scale is：It is 0 grade, disease-free；1 grade,
The loss less than 5% per fringe（Indivedual branch stalk morbidities）；3 grades, loss 6%-20% (morbidity of 1/3 or so branch stalk) per fringe；5 grades, the loss per fringe
21%-50% (Miho stems main shaft is fallen ill, and grain half is flat)；7 grades, the morbidity of loss 51%-70% (Miho stems, big portion's shrivelled kernel per fringe)；9 grades, often
Fringe loses 71%-100%（Miho stems are fallen ill, and cause dead ears).
Disease index=∑（The sick spike numbers at different levels × number of levels）×100/（Investigate total spike number × 9）
State of an illness inhibiting rate=（Check plot disease index-treatment region disease index）/ check plot disease index
Result of the test shows：The rice blast inhibiting rate for spraying the inducer of the inducing paddy rice blast resisting of the present embodiment 3 reaches
60.07%（See the table below）.
Embodiment 4：The inducer of inducing paddy rice blast resisting is prepared according to following steps
（1）Following components is taken respectively according to parts by weight：The sodium nitroprussiate 1.50g of mass concentration 99.5%, the salicylic acid of mass concentration 99.5%
7.50g, the chitosan oligosaccharide 3.00g of mass concentration 95%, the KI 1.50g of mass concentration 99.9%；
（2）Salicylic acid ethanol is dissolved, dissolving ratio is that the salicylic acid of 1.00g mass concentrations 99.5% 3ml ethanol dissolves；
（3）Sodium nitroprussiate water dissolves, dissolving ratio are dissolved for the sodium nitroprussiate 10ml of 1.00g mass concentrations 99.5%；
（4）To adding chitosan oligosaccharide in sodium nitroprusside solution, and carry out being sufficiently stirred for dissolving；
（5）To step（4）Mixing liquid in add KI, and be sufficiently stirred for dissolving；
（6）By step（2）The salicylic acid ethanol solution of gained adds step（5）Mixing liquid in, stir evenly mixing；
（7）To step（6）Mixed liquor in add a small amount of running water, stir evenly, and be dissolved to 100ml, obtain the anti-rice blast of inducing paddy rice
The inducer of disease.
The application method of the inducer of above-mentioned inducing paddy rice blast resisting is：In paddy rice beginning ear period, per 667m2Use 100ml
Inducer is uniformly sprayed in rice leaf surface to water 30kg, after stirring evenly, and every being sprayed again once after 7 days, consumption is identical.
Tested in Huai'an Agricultural Science and Technology Park within 2016, soil fertility is medium on the upper side, preceding stubble is wheat, experiment sets
It is processed as：The inducer of the inducing paddy rice blast resisting of the present embodiment 4 sprays treatment and compares（The anti-rice blast of inducing paddy rice is not sprayed
Sick inducer）, three repeat, and plot area is 20m2, rice varieties are Huaihe River rice No. 5, the seedling of May 15 in 2016, the machine of June 5
Rice transplanting, line-spacing 30cm, spacing in the rows 13cm, field rich water quality management, pest control measure are identical；On August 20th, 2016, foliage-spray
Once, sprayed again once every 7 days.In paddy rice dough stage（On September 27th, 2016）A situation arises for each treatment cell rice blast of investigation,
20 caves are investigated per cell, total spike number, morbidity spike number and the sick level of each cell investigation is recorded.Compareed with not dispenser according to treatment cell
The disease index of cell calculates relative inhibition of each treatment to rice blast.Rice blast grade scale is：It is 0 grade, disease-free；1 grade,
The loss less than 5% per fringe（Indivedual branch stalk morbidities）；3 grades, loss 6%-20% (morbidity of 1/3 or so branch stalk) per fringe；5 grades, the loss per fringe
21%-50% (Miho stems main shaft is fallen ill, and grain half is flat)；7 grades, the morbidity of loss 51%-70% (Miho stems, big portion's shrivelled kernel per fringe)；9 grades, often
Fringe loses 71%-100%（Miho stems are fallen ill, and cause dead ears).
Disease index=∑（The sick spike numbers at different levels × number of levels）×100/（Investigate total spike number × 9）
State of an illness inhibiting rate=（Check plot disease index-treatment region disease index）/ check plot disease index
Result of the test shows：The rice blast inhibiting rate for spraying the inducer of the inducing paddy rice blast resisting of the present embodiment 4 reaches
73.70%（See the table below）.Compared to other examples, this example formulations lures the effect of anti-rice blast best.
Table difference inducer is to rice blast inhibition
。
Claims (3)
1. the inducer of inducing paddy rice blast resisting, it is characterized in that：Wrapped in the component of the inducer of the inducing paddy rice blast resisting
Sodium nitroprussiate, salicylic acid, chitosan oligosaccharide, KI are included, the parts by weight of each component are in every 100ml inducers：Mass concentration 99.5%
Sodium nitroprussiate 0.75g~1.5g, the salicylic acid 3g of mass concentration 99.5%~7.5g, the 1.5~3g of chitosan oligosaccharide of mass concentration 95%, quality is dense
99.9% KI 1.0g~1.5g is spent, wherein salicylic acid ethanol dissolves, and other use water dissolves.
2. the inducer of inducing paddy rice blast resisting according to claim 1, it is characterized in that the anti-rice blast of the inducing paddy rice
The preparation method of the inducer of disease is comprised the following steps：
（1）Following components is taken respectively according to parts by weight：The sodium nitroprussiate 0.75g of mass concentration 99.5%~1.5g, mass concentration 99.5%
Salicylic acid 3g~7.5g, the chitosan oligosaccharide 1.5g of mass concentration 95%~3g, the KI 1.0g of mass concentration 99.9%~1.5g；
（2）Salicylic acid ethanol is dissolved, dissolving ratio is that the salicylic acid of 1g mass concentrations 99.5% 3ml ethanol dissolves；
（3）Sodium nitroprussiate water dissolves, dissolving ratio are dissolved for the sodium nitroprussiate 10ml of 1g mass concentrations 99.5%；
（4）To adding chitosan oligosaccharide in sodium nitroprusside solution, and carry out being sufficiently stirred for dissolving；
（5）To step（4）Mixing liquid in add KI, and be sufficiently stirred for dissolving；
（6）By step（2）The salicylic acid ethanol solution of gained adds step（5）Mixing liquid in, stir evenly mixing；
（7）To step（6）Mixed liquor in add a small amount of running water, stir evenly, and be dissolved to 100ml, obtain the anti-rice blast of inducing paddy rice
The inducer of disease.
3. the inducer of inducing paddy rice blast resisting according to claim 1, it is characterized in that the anti-rice blast of the inducing paddy rice
The application method of inducer of disease is：In paddy rice beginning ear period, per 667m2With 100ml inducers to water 30kg, uniform spray after stirring evenly
Rice leaf surface is imposed on, every being sprayed again once after 7 days, consumption is identical.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114041463A (en) * | 2021-11-09 | 2022-02-15 | 昆明学院 | Agents and method for reduplicating and reducing damage and improving mutagenesis rate of gynura bicolor chromosome |
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|---|---|---|---|---|
| CN101385470A (en) * | 2008-10-24 | 2009-03-18 | 吉林省农业科学院 | Novel environmental protection compound pesticide for preventing and treating rice blast |
| CN102365948A (en) * | 2011-10-28 | 2012-03-07 | 海南正业中农高科股份有限公司 | Chitosan oligosaccharide composition for resisting rice blast, application and method thereof |
| CN103918682A (en) * | 2014-04-01 | 2014-07-16 | 陕西西大华特科技实业有限公司 | Plant immunity bactericidal composition containing chitosan oligosaccharide and pyraclostrobin |
| CN106359451A (en) * | 2016-08-25 | 2017-02-01 | 河南农业大学 | Compound conditioning agent for delaying senility of leaves of wheat and/or improving yield, medicament comprising same, and application of same |
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2017
- 2017-04-19 CN CN201710256507.7A patent/CN106879631B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101385470A (en) * | 2008-10-24 | 2009-03-18 | 吉林省农业科学院 | Novel environmental protection compound pesticide for preventing and treating rice blast |
| CN102365948A (en) * | 2011-10-28 | 2012-03-07 | 海南正业中农高科股份有限公司 | Chitosan oligosaccharide composition for resisting rice blast, application and method thereof |
| CN103918682A (en) * | 2014-04-01 | 2014-07-16 | 陕西西大华特科技实业有限公司 | Plant immunity bactericidal composition containing chitosan oligosaccharide and pyraclostrobin |
| CN106359451A (en) * | 2016-08-25 | 2017-02-01 | 河南农业大学 | Compound conditioning agent for delaying senility of leaves of wheat and/or improving yield, medicament comprising same, and application of same |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114041463A (en) * | 2021-11-09 | 2022-02-15 | 昆明学院 | Agents and method for reduplicating and reducing damage and improving mutagenesis rate of gynura bicolor chromosome |
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