CN106879631B - The inducer of inducing paddy rice blast resisting - Google Patents
The inducer of inducing paddy rice blast resisting Download PDFInfo
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- CN106879631B CN106879631B CN201710256507.7A CN201710256507A CN106879631B CN 106879631 B CN106879631 B CN 106879631B CN 201710256507 A CN201710256507 A CN 201710256507A CN 106879631 B CN106879631 B CN 106879631B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/24—Cyanogen or compounds thereof, e.g. hydrogen cyanide, cyanic acid, cyanamide, thiocyanic acid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/12—Iodine, e.g. iodophors; Compounds thereof
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Abstract
The invention discloses a kind of inducers for luring anti-rice anti-rice blast, it include sodium nitroprussiate, salicylic acid, chitosan oligosaccharide, potassium iodide in the component of the inducer of the rice anti-rice blast, the parts by weight of each component in every 100ml inducer are as follows: 99.5% sodium nitroprussiate 0.75g~1.5g of mass concentration, 99.5% salicylic acid 3g~7.5g of mass concentration, 95% 1.5~3g of chitosan oligosaccharide of mass concentration, 99.9% potassium iodide 1.0g~1.5g of mass concentration, wherein salicylic acid is dissolved with ethyl alcohol, other to be dissolved with water.Inducer of the present invention is sprayed on rice leaf in rice beginning ear period, after being absorbed by blade, activates the activity of rice activity in vivo substance and enzyme, and that resists Pyricularia oryzae infects and inhibit Spot expansion, prevention and the generation and harm for reducing rice blast.
Description
Technical field
The present invention relates to a kind of inducers, and in particular to a kind of inducer of inducing paddy rice blast resisting, inducing paddy rice are anti-
Pyricularia oryzae infects, and prevents and reduce the generation of rice blast.
Background technique
Rice is the most important cereal crops of China, and rice is the staple food of Chinese 65% or more population.China or generation simultaneously
Rice Production amount and the maximum country of rice consumption figure in boundary, therefore Rice Production to guarantee Food Security in China and stablizes the world
Rice market all plays an important roll.Rice blast (Rice Blast) is caused by Pyricularia oryzae (Magnaporthe oryzae)
One of the rice disease of most serious, be distributed widely in each rice region in the world, there are different degrees of generation in Chinese South And North Rice Regions every year,
Hazard area and the extent of injury are larger, serious to hinder rice high yield stable yields.Cloudy weather for several days running, humidity are frequently encountered in growth period duration of rice
More dew, sultry weather conditions, will pandemic rice blast, cause disaster in a short time.According to the period and position caused harm
Difference can be divided into seedling pest, leaf pest, section pest, panicle blast and grain pest.It is general again larger with leaf pest, section pest and panicle blast.Currently,
The disease is mainly controlled by chemical prevention and plantation resistant variety, but due to the complexity of the disease pathogen microspecies heredity with
Pathogenic diversity, disease-resistant varieties often lose resistance after promoting the several years；Agro-chemicals control is due to being used for a long time pesticide
It is also easy to produce drug resistance, control efficiency is deteriorated, and increases pesticide dosage year by year, generates pollution to bad border, and persticide residue increases
Human health is adversely affected.Under the environmental condition for being suitable for Growth of Magnaporthe Grisea, it is big which will cause rice
The area underproduction, according to relevant report, the annual whole world because caused by rice blast Rice Yield Loss Caused account for about rice total output 10%~
15%, economic loss is up to tens dollars.In the plant disease epidemic time, general underproduction 10%-20%, more serious reachable 40%-50%, greatly
Even No kernels or seeds are gathered, as in a year of scarcity when generation.
Plant disease induced resistance refers to the defence system using plant itself, can be outer in initial introduced disease
The whole resistance level of the plant of boundary's factor excitation itself, the substance that crop can be excited to generate induced resistance reaction are then known as planting
Object disease inducer.It is that chemical pesticide prosperity and development cause inevitable outcome after adverse consequences, its small, environment with its dosage
The good advantage of compatibility will become effective substitute of the chemical pesticide of controlling plant diseases.
(such as Li Li, Liu Zhenjiao, Guo Xiaoli beta-aminobutyric acid inducing paddy rice blast resisting and the plant defenses enzyme activity such as Li Li
Property variation Jiangsu's agriculture science, 2011,39(2): be 185-186) research object, test result hair using beta-aminobutyric acid
Existing, the resilience of Pyricularia oryzae invasion can be enhanced in beta-aminobutyric acid.(Liang Liang, Hu Xuefang, Liu Weiping wait .20% to Liang Liang etc.
Oligomerization acid iodine to rice fall Wen Bing field control effect research China agronomy notification, 2013,29(36): 351-354) with 20%
Oligomerization acid iodine is research object, and test result is found, 20% oligomerization acid iodine falls Wen Bing with good control efficiency to rice.It contains
Handsome etc. (Sheng Jiao, Yi Yanmei, Feng Xian wait .1831SP inducing paddy rice blast resisting Study on mechanism Agriculture in Hunan scientific, and 2012
It (22): 33-34 is) research pair with 1831SP made of octadecyltrimethylammonium chloride and neopelex compounding
As test discovery, 1831SP is remarkably improved plant intracorporal PAL, POX, PPO, while significantly reducing the intracorporal CAT of plant
Activity resists the invasion of rice blast to enhance plant self-defense ability.Zhang Wenqing etc. (Zhang Wenqing, Feng Huafeng, Zuo Pingping,
The preparation of equal chitooligosaccharidequaternary quaternary ammonium salt and the Primary Study hubei agricultural science for inducing blast resisting, 2011,50(5) 942-945)
Using chitooligosaccharidequaternary quaternary ammonium salt as research object, as a result, it has been found that, chitooligosaccharidequaternary quaternary ammonium salt is sprayed application on rice shoot in rice seedling and can significantly be mentioned
Resistance of the high rice seedling to rice blast.(Wu Lamei, Bai Lianyang, Jin Chenzhong wait inducing paddy rice blast resisting to Wu Lamei etc.
Root of purple-flowered peucedanum inducer application technical research pre-test biological epidemics science, 2012,35(3): 320-322) boiled with root of purple-flowered peucedanum aqua, the root of purple-flowered peucedanum
Liquid, root of purple-flowered peucedanum original powder are research object, as a result, it has been found that, root of purple-flowered peucedanum aqua, root of purple-flowered peucedanum boil liquid, root of purple-flowered peucedanum original powder lure anti-rice anti-rice blast
Effect, wherein root of purple-flowered peucedanum aqua is apparently higher than root of purple-flowered peucedanum boil liquid and root of purple-flowered peucedanum original powder to the Induced resistant effect of rice blast.(the grandsons such as Sun Wanchun
Wan Chun, Xue Gaofeng, Zhang Jie wait influence of the silicon to rice defense key enzyme activity and its relationship plant battalion with blast resisting
Feeding and fertilizer journal, 2009,15(5) 1023-1028) using silicate as research object, as a result, it has been found that, applying silicon can significantly reduce water
The disease incidence and disease index of rice rice blast, control efficiency is up to 60.59%.(Liu Mingda, Zhang Wanmin the chemistry and life such as Liu Mingda
Good rice obtains the field efficacy Agricultural University Of Shenyang journal of inducing paddy rice blast resisting, and 2002,33(3): 188-190) with 8% good rice
Obtaining granule is research object, as a result, it has been found that, field rice is through chemistry and life --- after good rice must be handled, has to rice blast
Certain resistance.
The inducer of anti-crop disease-resistant is being lured to apply for a patent aspect, some experts have also applied for that related patents are protected.
Du Yu light etc. has applied for " a kind of plant induced resistance agent " (patent No. ZL99122582.1), which disclose: it provides a kind of logical
The enzymic degradation crossed under microbial fermentation combination certain condition obtains the plant induced resistance agent with bioactive ingredients such as oligosaccharides, and
Applied to soybean, cotton, tobacco, brinjal plant disease." a kind of inducer is passed in control garden crop soil for the application such as Li Baoju
Application in disease " (patent No. ZL201010117498.1), which disclose: with phendioxin, 2,3- thiadiazoles -7- formic acid
Trifluoro ethyl ester lures anti-yellowing melon anti-blight, samping off, the anti-root-rot type epidemic disease of capsicum, the anti-root rot of cyclamen, horse as inducer
The anti-shot hole of bell potato, oyster mushroom bacteria resistance brown spot.Zhang Shanxue etc. has applied for a kind of " chitosan oligosaccharide group for anti-rice blast
Close object and application thereof and method " (patent No. ZL201110333070.5), which disclose: one kind being used for anti-rice blast
Composition pesticide, include chitosan oligosaccharide in the composition pesticide, the weight percent that chitosan oligosaccharide accounts for the composition pesticide is
0.005%-0.02%.The application such as Ruan Songlin " a kind of plant immune inducer and its application " (application No. is
201510263261.7), which disclose: the immune inducer percentage, effective component includes: 0.001%-
0.02% amino-oligosaccharide and the phosphite of 0.01%-0.2% are applied to the anti-helminthosporium maydis of inducing maize.The application such as Ruan Songlin
" a kind of application of plant immune inducer in prevention and treatment downy mildew of garpe and anthracnose " (application No. is 201510264420.5),
Which disclose: the immune inducer percentage, effective component includes: the amino-oligosaccharide of 0.001%-0.02%
With the phosphite of 0.01%-0.2%, it is applied to induction grape downy mildew resistance and anthracnose.Ruan Songlin etc. has applied for " plant immune
Application of the inducer in prevention and treatment cucumber samping off and damping-off " (application No. is 201610111820.7), which disclose:
The immune inducer percentage, effective component includes: the amino-oligosaccharide of 0.001%-0.025%, 0.01%-0.25%
Phosphate, 0.01%-0.25% phosphite, be applied to inducing cucumber resisting damping-off and damping-off.Apply the application such as great waves
" a kind of method that spermine induction processing improves rice bacterial blight resistance and blast resisting " (application No. is 201510706290.6),
Which disclose: a kind of method that spermine induction processing improves rice bacterial blight resistance and blast resisting, this method includes kind
It plants ground preparation, seedling management and spermine and induces processing step." a kind of inducer is answered in agricultural chemicals suspension agent for the application such as Peng Shuming
With technology " (application No. is 201610319376.8), which disclose: in common agriculture chemicals fungicide suspending agent 1%- is added
30% inducer, the inducer are made of salicylic acid and beta-aminobutyric acid, and the weight fraction ratio of salicylic acid and beta-aminobutyric acid is
1:100-100:1, induction plant generate resistance to a variety of pathogens, reduce pesticide dosage.
Summary of the invention
The object of the present invention is to provide a kind of inducers of inducing paddy rice blast resisting, uniformly spray in rice beginning ear period
In rice leaf, the activity of rice activity in vivo substance and enzyme is activated, infecting for Pyricularia oryzae is resisted, prevents and reduce rice blast
Generation, the harm for avoiding and mitigating rice blast to rice.
The technical solution of the invention is as follows: including that nitre is general in the component of the inducer of the inducing paddy rice wheat blast resisting
Sodium, salicylic acid, chitosan oligosaccharide, potassium iodide, the parts by weight of each component in every 100ml inducer are as follows: 99.5% sodium nitroprussiate of mass concentration
0.75g~1.5g, 99.5% salicylic acid 3g~7.5g of mass concentration, 95% 1.5~3g of chitosan oligosaccharide of mass concentration, mass concentration
99.9% potassium iodide 1.0g~1.5g, wherein salicylic acid is dissolved with ethyl alcohol, other to be dissolved with water.
The preparation method of the inducer of above-mentioned inducing paddy rice blast resisting the following steps are included:
(1) following components is taken respectively according to parts by weight: 99.5% sodium nitroprussiate 0.75g~1.5g of mass concentration, mass concentration
99.5% salicylic acid 3g~7.5g, 95% chitosan oligosaccharide 1.5g~3g of mass concentration, 99.9% potassium iodide 1.0g~1.5g of mass concentration；
(2) salicylic acid is dissolved with ethyl alcohol, dissolving ratio is that 99.5% salicylic acid of 1g mass concentration 3ml ethyl alcohol dissolves；
(3) sodium nitroprussiate is dissolved with water, dissolving ratio is that 99.5% sodium nitroprussiate of 1g mass concentration is dissolved with 10ml；
(4) chitosan oligosaccharide is added into sodium nitroprusside solution, and carries out that dissolution is sufficiently stirred；
(5) potassium iodide is added into the mixing liquid of step (4), and carries out that dissolution is sufficiently stirred；
(6) the resulting salicylic acid ethanol solution of step (2) is added in the mixing liquid of step (5), stirs evenly mixing；
(7) a small amount of tap water is added into the mixed liquor of step (6), stirs evenly, and is dissolved to 100ml, it is anti-to obtain inducing paddy rice
The inducer of rice blast.
The application method of the inducer of above-mentioned inducing paddy rice blast resisting is: in rice beginning ear period, every 667m2Use 100ml
Inducer is uniformly sprayed on rice leaf surface to water 30kg after stirring evenly, spray again after 7 days once, dosage is identical.
Compared with prior art, the present invention has following obvious advantage:
One, the inducer of inducing paddy rice blast resisting of the invention is sprayed on rice leaf in rice beginning ear period, by blade
It absorbs, activates the activity of rice activity in vivo substance and some enzymes, be effective against the intrusion of Pyricularia oryzae and the extension of scab, from
And prevent and reduce the generation of rice blast, mitigate harm of the rice blast to rice, reduces the usage amount of chemical pesticide, protection agricultural
Ecological environment and human health.
Two, the salicylic acid in the present invention and chitosan oligosaccharide all have the activity of enzyme and active material in activation rice body, resist
The intrusion of Pyricularia oryzae and the effect for inhibiting Spot expansion compound superposition synergy by the two, reduce each plant demand, reduce at
This.
Three, the sodium nitroprussiate in the present invention is the foreign donor of NO, is generated a large amount of NO after rice absorbing, NO by with
The iron ion of its soluble recepter guanylate cyclase (guanylatecyclase, GC) combines, and changes the stereochemical structure of GC
To improve its activity, and the increase that intracellular second messenger's ring cytimidine list nucleoside phosphorylase (cGMP) generates is further resulted in,
The cGMP of formation acts on phenylalanine ammonia lyase (PAL) again and adjusts the accumulation of salicylic acid (SA), thus inducing paddy rice PAL
With the expression of the disease-resistant related genes such as pathogenesis-related proteins (PR-1), and then disease-resistant (rice blast) reaction of rice is participated in.
Four, on the one hand the potassium iodide in the present invention is made by being absorbed after foliage-spray by rice leaf by osmotic adjustment
With reduction pathogen spore germination；On the other hand it improves the content of the intracorporal calcium ion of rice and promotes the signals such as salicylic acid point
The dynamic accumulation of son accelerates the increase of local cell death and cell leakage, signal is further amplified, these signals promote again
Into the activity of relevant enzyme in the metabolic processes such as active oxygen, phenolic substances, to increase paddy disease-resistant (rice blast) property.
Five, rice leaf is sprayed on rice beginning ear period with the inducer of inducing paddy rice blast resisting of the invention, wherein
Salicylic acid, chitosan oligosaccharide, sodium nitroprussiate, potassium iodide mutually act synergistically, to rice head blight inhibiting rate up to 48.01% or more, have
Preferable economic benefit.
Six, the inducer of rice anti-rice blast of the invention is a kind of novel prevention and the reduction pathogenetic production of rice blast
Product can excite plant immune system, play disease-resistant, diseases prevention purpose；Itself has no bactericidal activity, but it can excite plant to produce
Raw systemic acquired resistance, excites the immunologic mechanism of inside plants, the high yield and high quality potentiality of rice is made to be not fully exerted, and ensures
The production of rice and edible safety protect human health.
Specific embodiment
Technical solution of the invention is further illustrated combined with specific embodiments below, these embodiments should not be understood as
It is the limitation to technical solution.
Embodiment 1: the inducer of inducing paddy rice blast resisting is prepared according to following steps
(1) following components is taken respectively according to parts by weight: 99.5% sodium nitroprussiate 0.75g of mass concentration, 99.5% water of mass concentration
Poplar acid 3.00g, 95% chitosan oligosaccharide 1.50g of mass concentration, 99.9% potassium iodide 1.00g of mass concentration；
(2) salicylic acid is dissolved with ethyl alcohol, dissolving ratio is that 99.5% salicylic acid of 1.00g mass concentration is molten with 3ml ethyl alcohol
Solution；
(3) sodium nitroprussiate is dissolved with water, dissolving ratio is that 99.5% sodium nitroprussiate of 1.00g mass concentration is dissolved with 10ml；
(4) chitosan oligosaccharide is added into sodium nitroprusside solution, and carries out that dissolution is sufficiently stirred；
(5) potassium iodide is added into the mixing liquid of step (4), and carries out that dissolution is sufficiently stirred；
(6) the resulting salicylic acid ethanol solution of step (2) is added in the mixing liquid of step (5), stirs evenly mixing；
(7) a small amount of tap water is added into the mixed liquor of step (6), stirs evenly, and is dissolved to 100ml, it is anti-to obtain inducing paddy rice
The inducer of rice blast.
The application method of the inducer of above-mentioned inducing paddy rice blast resisting is: in rice beginning ear period, every 667m2Use 100ml
Inducer is uniformly sprayed on rice leaf surface to water 30kg after stirring evenly, spray again after 7 days once, dosage is identical.
It is tested in Huai'an Agricultural Science and Technology Park within 2016, soil fertility is medium on the upper side, and preceding stubble is wheat, and test is set
Processing are as follows: the inducer of the inducing paddy rice blast resisting of the present embodiment 1 sprays processing and control (does not spray the anti-rice blast of inducing paddy rice
Sick inducer), three repeat, plot area 20m2, rice varieties be Huaihe River rice No. 5, seedling on May 15 in 2016, June 5 machine
Rice transplanting, line-spacing 30cm, spacing in the rows 13cm, field fertilizer and water management, pest control measure are identical；On August 20th, 2016, foliage-spray
Once, it was sprayed again every 7 days primary.Investigating each processing cell rice blast in rice dough stage (on September 27th, 2016), a situation arises,
Every cell investigates 20 caves, records the total spike number, morbidity spike number and sick grade of each cell investigation.It is compareed according to processing cell with not being administered
The disease index of cell calculates each processing to the opposite inhibitory effect of rice blast.Rice blast grade scale are as follows: 0 grade, disease-free；1 grade,
Every 5% or less fringe loss (individual branch stalk morbidities)；3 grades, every fringe loss 6%-20% (morbidity of 1/3 or so branch stalk)；5 grades, every fringe loss
The morbidity of 21%-50% (Miho stem main shaft, grain half are flat)；7 grades, every fringe loss 51%-70% (Miho stem morbidity, big portion's shrivelled kernel)；9 grades, often
Fringe loses the morbidity of 71%-100%(Miho stem, causes dead ears).
Disease index=∑ (disease spike number × number of levels at different levels) × 100/(investigates total spike number × 9)
State of an illness inhibiting rate=(check plot disease index-treatment region disease index)/check plot disease index
Test result shows: the rice blast for spraying the inducer of the inducing paddy rice blast resisting of the present embodiment 1 inhibits
Rate see the table below up to 48.01%().
Embodiment 2: the inducer of inducing paddy rice blast resisting is prepared according to following steps
(1) following components is taken respectively according to parts by weight: 99.5% sodium nitroprussiate 1.00g of mass concentration, 99.5% water of mass concentration
Poplar acid 4.50g, 95% chitosan oligosaccharide 2.00g of mass concentration, 99.9% potassium iodide 1.25g of mass concentration；
(2) salicylic acid is dissolved with ethyl alcohol, dissolving ratio is that 99.5% salicylic acid of 1.00g mass concentration is molten with 3ml ethyl alcohol
Solution；
(3) sodium nitroprussiate is dissolved with water, dissolving ratio is that 99.5% sodium nitroprussiate of 1.00g mass concentration is dissolved with 10ml；
(4) chitosan oligosaccharide is added into sodium nitroprusside solution, and carries out that dissolution is sufficiently stirred；
(5) potassium iodide is added into the mixing liquid of step (4), and carries out that dissolution is sufficiently stirred；
(6) the resulting salicylic acid ethanol solution of step (2) is added in the mixing liquid of step (5), stirs evenly mixing；
(7) a small amount of tap water is added into the mixed liquor of step (6), stirs evenly, and is dissolved to 100ml, it is anti-to obtain inducing paddy rice
The inducer of rice blast.
The application method of the inducer of above-mentioned inducing paddy rice blast resisting is: in rice beginning ear period, every 667m2Use 100ml
Inducer is uniformly sprayed on rice leaf surface to water 30kg after stirring evenly, spray again after 7 days once, dosage is identical.
It is tested in Huai'an Agricultural Science and Technology Park within 2016, soil fertility is medium on the upper side, and preceding stubble is wheat, and test is set
Processing are as follows: the inducer of the inducing paddy rice blast resisting of the present embodiment 2 sprays processing and control (does not spray the anti-rice blast of inducing paddy rice
Sick inducer), three repeat, plot area 20m2, rice varieties be Huaihe River rice No. 5, seedling on May 15 in 2016, June 5 machine
Rice transplanting, line-spacing 30cm, spacing in the rows 13cm, field fertilizer and water management, pest control measure are identical；On August 20th, 2016, foliage-spray
Once, it was sprayed again every 7 days primary.Investigating each processing cell rice blast in rice dough stage (on September 27th, 2016), a situation arises,
Every cell investigates 20 caves, records the total spike number, morbidity spike number and sick grade of each cell investigation.It is compareed according to processing cell with not being administered
The disease index of cell calculates each processing to the opposite inhibitory effect of rice blast.Rice blast grade scale are as follows: 0 grade, disease-free；1 grade,
Every 5% or less fringe loss (individual branch stalk morbidities)；3 grades, every fringe loss 6%-20% (morbidity of 1/3 or so branch stalk)；5 grades, every fringe loss
The morbidity of 21%-50% (Miho stem main shaft, grain half are flat)；7 grades, every fringe loss 51%-70% (Miho stem morbidity, big portion's shrivelled kernel)；9 grades, often
Fringe loses the morbidity of 71%-100%(Miho stem, causes dead ears).
Disease index=∑ (disease spike number × number of levels at different levels) × 100/(investigates total spike number × 9)
State of an illness inhibiting rate=(check plot disease index-treatment region disease index)/check plot disease index
Test result shows: the rice blast for spraying the inducer of the inducing paddy rice blast resisting of the present embodiment 2 inhibits
Rate see the table below up to 55.25%().
Embodiment 3: the inducer of inducing paddy rice blast resisting is prepared according to following steps
(1) following components is taken respectively according to parts by weight: 99.5% sodium nitroprussiate 1.25g of mass concentration, 99.5% water of mass concentration
Poplar acid 6.00g, 95% chitosan oligosaccharide 3.00g of mass concentration, 99.9% potassium iodide 1.00g of mass concentration；
(2) salicylic acid is dissolved with ethyl alcohol, dissolving ratio is that 99.5% salicylic acid of 1.00g mass concentration is molten with 3ml ethyl alcohol
Solution；
(3) sodium nitroprussiate is dissolved with water, dissolving ratio is that 99.5% sodium nitroprussiate of 1.00g mass concentration is dissolved with 10ml；
(4) chitosan oligosaccharide is added into sodium nitroprusside solution, and carries out that dissolution is sufficiently stirred；
(5) potassium iodide is added into the mixing liquid of step (4), and carries out that dissolution is sufficiently stirred；
(6) the resulting salicylic acid ethanol solution of step (2) is added in the mixing liquid of step (5), stirs evenly mixing；
(7) a small amount of tap water is added into the mixed liquor of step (6), stirs evenly, and is dissolved to 100ml, it is anti-to obtain inducing paddy rice
The inducer of rice blast.
The application method of the inducer of above-mentioned inducing paddy rice blast resisting is: in rice beginning ear period, every 667m2Use 100ml
Inducer is uniformly sprayed on rice leaf surface to water 30kg after stirring evenly, spray again after 7 days once, dosage is identical.
It is tested in Huai'an Agricultural Science and Technology Park within 2016, soil fertility is medium on the upper side, and preceding stubble is wheat, and test is set
Processing are as follows: the inducer of the inducing paddy rice blast resisting of the present embodiment 3 sprays processing and control (does not spray the anti-rice blast of inducing paddy rice
Sick inducer), three repeat, plot area 20m2, rice varieties be Huaihe River rice No. 5, seedling on May 15 in 2016, June 5 machine
Rice transplanting, line-spacing 30cm, spacing in the rows 13cm, field fertilizer and water management, pest control measure are identical；On August 20th, 2016, foliage-spray
Once, it was sprayed again every 7 days primary.Investigating each processing cell rice blast in rice dough stage (on September 27th, 2016), a situation arises,
Every cell investigates 20 caves, records the total spike number, morbidity spike number and sick grade of each cell investigation.It is compareed according to processing cell with not being administered
The disease index of cell calculates each processing to the opposite inhibitory effect of rice blast.Rice blast grade scale are as follows: 0 grade, disease-free；1 grade,
Every 5% or less fringe loss (individual branch stalk morbidities)；3 grades, every fringe loss 6%-20% (morbidity of 1/3 or so branch stalk)；5 grades, every fringe loss
The morbidity of 21%-50% (Miho stem main shaft, grain half are flat)；7 grades, every fringe loss 51%-70% (Miho stem morbidity, big portion's shrivelled kernel)；9 grades, often
Fringe loses the morbidity of 71%-100%(Miho stem, causes dead ears).
Disease index=∑ (disease spike number × number of levels at different levels) × 100/(investigates total spike number × 9)
State of an illness inhibiting rate=(check plot disease index-treatment region disease index)/check plot disease index
Test result shows: the rice blast for spraying the inducer of the inducing paddy rice blast resisting of the present embodiment 3 inhibits
Rate see the table below up to 60.07%().
Embodiment 4: the inducer of inducing paddy rice blast resisting is prepared according to following steps
(1) following components is taken respectively according to parts by weight: 99.5% sodium nitroprussiate 1.50g of mass concentration, 99.5% water of mass concentration
Poplar acid 7.50g, 95% chitosan oligosaccharide 3.00g of mass concentration, 99.9% potassium iodide 1.50g of mass concentration；
(2) salicylic acid is dissolved with ethyl alcohol, dissolving ratio is that 99.5% salicylic acid of 1.00g mass concentration is molten with 3ml ethyl alcohol
Solution；
(3) sodium nitroprussiate is dissolved with water, dissolving ratio is that 99.5% sodium nitroprussiate of 1.00g mass concentration is dissolved with 10ml；
(4) chitosan oligosaccharide is added into sodium nitroprusside solution, and carries out that dissolution is sufficiently stirred；
(5) potassium iodide is added into the mixing liquid of step (4), and carries out that dissolution is sufficiently stirred；
(6) the resulting salicylic acid ethanol solution of step (2) is added in the mixing liquid of step (5), stirs evenly mixing；
(7) a small amount of tap water is added into the mixed liquor of step (6), stirs evenly, and is dissolved to 100ml, it is anti-to obtain inducing paddy rice
The inducer of rice blast.
The application method of the inducer of above-mentioned inducing paddy rice blast resisting is: in rice beginning ear period, every 667m2Use 100ml
Inducer is uniformly sprayed on rice leaf surface to water 30kg after stirring evenly, spray again after 7 days once, dosage is identical.
It is tested in Huai'an Agricultural Science and Technology Park within 2016, soil fertility is medium on the upper side, and preceding stubble is wheat, and test is set
Processing are as follows: the inducer of the inducing paddy rice blast resisting of the present embodiment 4 sprays processing and control (does not spray the anti-rice blast of inducing paddy rice
Sick inducer), three repeat, plot area 20m2, rice varieties be Huaihe River rice No. 5, seedling on May 15 in 2016, June 5 machine
Rice transplanting, line-spacing 30cm, spacing in the rows 13cm, field fertilizer and water management, pest control measure are identical；On August 20th, 2016, foliage-spray
Once, it was sprayed again every 7 days primary.Investigating each processing cell rice blast in rice dough stage (on September 27th, 2016), a situation arises,
Every cell investigates 20 caves, records the total spike number, morbidity spike number and sick grade of each cell investigation.It is compareed according to processing cell with not being administered
The disease index of cell calculates each processing to the opposite inhibitory effect of rice blast.Rice blast grade scale are as follows: 0 grade, disease-free；1 grade,
Every 5% or less fringe loss (individual branch stalk morbidities)；3 grades, every fringe loss 6%-20% (morbidity of 1/3 or so branch stalk)；5 grades, every fringe loss
The morbidity of 21%-50% (Miho stem main shaft, grain half are flat)；7 grades, every fringe loss 51%-70% (Miho stem morbidity, big portion's shrivelled kernel)；9 grades, often
Fringe loses the morbidity of 71%-100%(Miho stem, causes dead ears).
Disease index=∑ (disease spike number × number of levels at different levels) × 100/(investigates total spike number × 9)
State of an illness inhibiting rate=(check plot disease index-treatment region disease index)/check plot disease index
Test result shows: the rice blast for spraying the inducer of the inducing paddy rice blast resisting of the present embodiment 4 inhibits
Rate see the table below up to 73.70%().Compared to other examples, this example formulations lures the effect of anti-rice blast best.
Table difference inducer is to rice blast inhibitory effect
。
Claims (3)
1. the inducer of inducing paddy rice blast resisting, it is characterized in that: being wrapped in the component of the inducer of the inducing paddy rice blast resisting
Sodium nitroprussiate, salicylic acid, chitosan oligosaccharide, potassium iodide are included, the parts by weight of each component in every 100ml inducer are as follows: mass concentration 99.5%
Sodium nitroprussiate 0.75g~1.5g, 99.5% salicylic acid 3g~7.5g of mass concentration, 95% 1.5~3g of chitosan oligosaccharide of mass concentration, quality are dense
99.9% potassium iodide 1.0g~1.5g is spent, wherein salicylic acid is dissolved with ethyl alcohol, other to be dissolved with water.
2. the inducer of inducing paddy rice blast resisting according to claim 1, it is characterized in that the anti-rice blast of the inducing paddy rice
Disease inducer preparation method the following steps are included:
(1) following components is taken respectively according to parts by weight: 99.5% sodium nitroprussiate 0.75g~1.5g of mass concentration, mass concentration 99.5%
Salicylic acid 3g~7.5g, 95% chitosan oligosaccharide 1.5g~3g of mass concentration, 99.9% potassium iodide 1.0g~1.5g of mass concentration；
(2) salicylic acid is dissolved with ethyl alcohol, dissolving ratio is that 99.5% salicylic acid of 1g mass concentration 3ml ethyl alcohol dissolves；
(3) sodium nitroprussiate is dissolved with water, dissolving ratio is that 99.5% sodium nitroprussiate of 1g mass concentration is dissolved with 10ml；
(4) chitosan oligosaccharide is added into sodium nitroprusside solution, and carries out that dissolution is sufficiently stirred；
(5) potassium iodide is added into the mixing liquid of step (4), and carries out that dissolution is sufficiently stirred；
(6) the resulting salicylic acid ethanol solution of step (2) is added in the mixing liquid of step (5), stirs evenly mixing；
(7) a small amount of tap water is added into the mixed liquor of step (6), stirs evenly, and is dissolved to 100ml, obtains the anti-rice blast of inducing paddy rice
The inducer of disease.
3. the inducer of inducing paddy rice blast resisting according to claim 1, it is characterized in that the anti-rice blast of the inducing paddy rice
The application method of the inducer of disease is: in rice beginning ear period, every 667m2With 100ml inducer to water 30kg, uniformly spray after stirring evenly
Rice leaf surface is imposed on, is sprayed again after 7 days once, dosage is identical.
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| CN102365948B (en) * | 2011-10-28 | 2013-03-20 | 海南正业中农高科股份有限公司 | Chitosan oligosaccharide composition for resisting rice blast, application and method thereof |
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