CN105660698B - Application of the plant immune inducer in prevention cucumber samping off and damping-off - Google Patents
Application of the plant immune inducer in prevention cucumber samping off and damping-off Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/26—Phosphorus; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The invention discloses a kind of application of plant immune inducer in prevention cucumber samping off and damping-off, the active ingredient of the plant immune inducer, percentage, the plant immune inducer includes：0.001~0.025% amino-oligosaccharide, 0.01~0.25% phosphite and 0.01~0.25% phosphate.Amino-oligosaccharide, phosphite and phosphate are combined by the present invention, the immune inducer each component mixed proportion obtained is reasonable, it is not only good to the control effect of cucumber samping off and cucumber rhizoctonia rot disease, but also the emergence rate, planting percent and Seedling Quality of cucumber can be improved.
Description
Technical field
The present invention relates to biological pesticide technical fields more particularly to a kind of plant immune inducer in prevention cucumber samping off
With the application in damping-off.
Background technology
Cucumber can endanger in entire growing process by a variety of pathogens, and wherein samping off and damping-off are that winter-spring season is yellow
Melon seedling phase Major Diseases.
It is mostly melon and fruit corruption to cause the cause of disease of cucumber samping offMould(Pythium aphanidermatum(Eds.)
Fitzp), belong to Mastigomycotina fungi；Mycelium flocculence, white, mycelium is without diaphragm, 2.3~7.1 μm of diameter；Sporangium
Filiform, size (63~725) μ m (4.9~14.8) μm；Oogonium is spherical, 14.9~34.8 μm of diameter.OvumSporeSpherical shape is put down
It is sliding, 14.0~22.0 μm of diameter.
Pathogen is septic very strong, can be overwintering in diseased plant residuum and in soil long-term survival in the soil.Condition is suitable
When, germination of cucumber seeds, pathogen infection melon and fruit causes to damping off；Germ can also by pour water or rainwater punching splash propagation.After the onset
Seedling basal part of stem will appear khaki water stain shape scab, sick portion easily shortens threadiness into after rapidly developing, and cotyledon is also bud green, seedling
With regard to the ground that lodges.When seedbed humidity is big, sick portion generates one layer of white hypha, and last sick seedling is rotten or withered.
Cucumber samping off control method：(1) seed disinfection.It is impregnated 10 minutes with 55 DEG C of warm water before sowing, it also can be by seed
It immerses in 500 times of carbendazol wettable powder solution 30 minutes.It is rinsed well after seed soaking, then carries out vernalization sowing.(2) bed soil disappears
Poison.When configuring Nutrition Soil, 100 grams of carbendazim are added in every cubic metre of soil.After the watering of seedbed, 72.2% is sprayed before sowing and general is made every effort to overcome
500 times of liquid are sprayed one time again after earthing.(3) chemical control.Early stage can spray 72.2% it is general make every effort to overcome 500 times of liquid of aqua, or
500 times of liquid of 95% 3000 times of hymexazol fine work liquid or 64% Sandofan wettable powder or 70% Mancozeb wettable powder
500 times of liquid of 600 times of liquid of 500 times of liquid of agent or 75% Bravo wettable powder or 50% carbendazol wettable powder.Spray value
It is big, it is desirable that the bed soil around moistening melon seedling, spray in 7 to 10 days is primary, even spray 2 to 3 times.Also can by every square metre of above-mentioned medicament with
10 to 15 grams of medicine mixes appropriate fine earth or fine sand, after seed bud is unearthed, removes mulch in time, and it is anti-that one layer of medicine soil is uniformly spread to bed surface
It controls.
Cucumber rhizoctonia rot cause of disease is Rhizoctonia solani Kuhn Rhizoctonia solaniK ü hn, belongs to Deuteromycotina.Found withered silk
Pyrenomycetes is soil inhabitant, and mainly the invalid body and soil long-term survival with mycelium or sclerotium in soil, can also be mixed in
It is overwintering without surviving in completely decomposed compost；Sclerotium crineous, unsetting, quality is loose, rough surface；Only a few is with mycelia
Body is hidden in seed more；Germ is overwintering in the soil, can be 2~3 years saprophytic in soil；It is propagated by flow, farm implements.Germ sends out
It is 24 DEG C to educate thermophilic, and sowing is overstocked, and not in time, temperature is excessively high and falls ill for thinning.
The control method of cucumber rhizoctonia rot：(1) cultural control：Seedbed hydrofuge cooling, reinforces ventilation, increases illumination；(2) object
Reason prevention：With 55 DEG C of water seed soaking 15min；(3) chemical control：Spray 400 times of liquid of Cal-cop 10 or 70% Mancozeb 500
Times 600 times of liquid of liquid or 75% Bravo, or with 1000 times of liquid sprays of 25% metalaxyl, 1000 times of liquid or 70% fenaminosulf.
Though can effectively prevent cucumber at seedling stage disease using chemical pesticide, pesticide residue and food in cucumber fruits are also increased
Product security risk and ecological environment hidden danger, result of study show that the inducer using nontoxic residue-free can lure into plant and produce
Raw disease resistance response, to make plant obtain system resistant from disease or mitigate disease.Therefore, not using immune inducer
It is a kind of both safe and effective method of control cucumber disease to lose.
Inducer can be divided into non-biological origin and biological source two major classes at present.The inducer of non-biological origin includes inorganic
Salt (copper sulphate etc.), organic acid (salicylic acid etc.) and the oligosaccharide kind obtained with the method for organic synthesis.The inducer of biological source
Further include viral capsid proteins, sugar other than having plant-derived oligogalactose and anaphylactin (harpin) from bacterium
The inducers such as protide.
Immune inducer mainly by exciting the disease resistance of plant itself, makes it generate antibacterial material, inhibits to reach
The purpose of pathogen.This resistance has many advantages, such as wide spectrum, stabilization, lasting, and can solving chemical prevention, there are the anti-medicines of pathogen
Property, environmental pollution and to people and animals' side-effect problem, realize agricultural product hamlessizing technology, it has also become the new tool of Plant disease control.Cause
This, disease-resistant, the pest-resistant plant immune inducer of various excitation plant autoprotcrtive systems has become the new of biological agent research and development
Hot spot.
Currently, registered such preparation has in the world：The Messenger of the Eden Biosciences companies in the U.S.,
The Keyplex of Oxycom, Morse company of Redox Chemicals companies, the Actigard of Syngenta Co., Ltd of Switzerland, Japan
The NCI of chemical drug company, the Chitosan etc. of rising sun chemical company of South Korea.In recent years, China has been developed that the plant of protide, oligosaccharide kind
Inducer is immunized in object, Field information the result shows that, this two kinds of inducers show good disease-resistant effect to droop, virosis
Fruit, at the same it is with obvious effects to improving melon, fruit color, sugariness, fruit retention etc..For many years studies have shown that plant starts albumen
To tomato, capsicum, eggplant, seeding corn and other crops virosis control effect up to 60% or more.In entire crop growth period, plant is used
Object, which starts albumen, can reduce conventional pesticide usage amount 50%-60%, reduce Fertilizer application amount 20%-30%.
It discloses application No. is 201410668404.8 application for a patent for invention and a kind of there is the immune biology for luring anti-effect
Pesticide, in parts by weight, active ingredient include 1~20 part of 0.2~12 part of amino-oligosaccharide and brassin lactones.The life
Object pesticide can be used for preventing plant anthracnose.
In addition, according to have disclosed can the immune exciton of activated plant, make agriculture by different types of exciton is mixed
Also there is much the research of each component synergy in medicine.Such as：Early in 1986, Davis had found poly- seven sugar in Portugal and endogenous poly
Polygalacturonate lyase, ten polygalacturonic acids, sodium acetate, sodium formate are mixed with sodium propionate, have synergistic function；Portugal
It is poly- seven sugar it is mixed with endogenous polygalacturonase lyases, ten polygalacturonic acids when induced activity than individually inducing when lure
It leads the sum of activity and improves 35 times；Beta-aminobutyric acid (BABA) is mixed with rhizobium (PGPR) of plant growth is promoted, and there is interaction to increase
Effect acts on；Sun Yanqiu etc. has found the fungi polysaccharide and pectin polysaccharide of artificial synthesized plant induced resistance agent glucohexaose and artificial fermentation
Mixture can induce the increase of cucumber, tomato disease resistance, and the preventive effect of mixed sugar is better than the processing of each single dose；Yamaguchi
Research finds that poly- seven sugar of two kinds of oligosaccharides exciton N- acetyl shells is mixed with the poly- pentasaccharides in Portugal, can improve disease resistance, internal antibacterial
The content of substance rice husk ketone is 5.5 times of independent excitation induction；Pass through poly- seven sugar of the N- acetyl shells of isotope labelling and rice
The affinity of cytoplasma membrane receptor inhibits experiment, it is found that there are two different receptors for two exciton tools.
However, at present about using plant induced resistance agent come prevent cucumber at seedling stage disease research report it is also less, using not
It is even more to be rarely reported that same type exciton, which prepares plant induced resistance agent applied to diseases researchs such as cucumber samping off, damping-offs,.
Invention content
The present invention provides a kind of application of plant immune inducer in prevention cucumber samping off and damping-off, this is immune
Inducer mixed proportion is reasonable, good for the control effect of cucumber at seedling stage disease, and can improve the emergence rate of cucumber, planting percent
And Seedling Quality.
The present invention provides a kind of application of plant immune inducer in preventing cucumber samping off, wherein by weight hundred
The active ingredient of score meter, the plant immune inducer includes：0.001~0.025% amino-oligosaccharide, 0.01~
0.25% phosphite and 0.01~0.25% phosphate.
The present invention also provides a kind of application of plant immune inducer in preventing cucumber rhizoctonia rot, wherein by weight
The active ingredient of percentage meter, the plant immune inducer includes：0.001~0.025% amino-oligosaccharide, 0.01~
0.25% phosphite and 0.01~0.25% phosphate.Amino-oligosaccharide is capable of the immune system of activated plant and growth is
System makes to generate the active material with resistant effect in plant, inhibits the intrusion of pathogen, promote the growth and development of plant.
Preferably, percentage, active ingredient are：0.005~0.012% amino-oligosaccharide, 0.05~
0.12% phosphite and 0.05~0.12% phosphate.
Preferably, the phosphite is potassium dihydrogen phosphite, the phosphate is potassium dihydrogen phosphate
(KH2PO4)。
Potassium dihydrogen phosphite (KH2PO3) it is in subacidity, there is the dual work killed pathogen and plant is induced to generate resistance
With the mechanism of phosphorous acid controlling disease includes：Directly protect host's by interfering and inhibiting the growth of germ mycelia with production born of the same parents
Effect, and accelerate indirect effect of generation and the accumulation of phenolate object or other disease-resistance substances in plant.
Potassium dihydrogen phosphate in acidity, with notable increasing both production and income, the amount of changing optimization crop quality, resistant to lodging, disease and insect resistance,
Prevent many excellent effects such as early ageing, and with overcome plant growth later stage root system aging absorbability decline caused by nutrition
Insufficient effect.
The plant immune inducer further includes other components, specially 0.001~0.02% surfactant；
0.001~0.02% sustained release agent.It is further preferred that 0.001~0.012% surfactant；0.001~0.012% sustained release
Agent.Surfactant can improve distribution, attachment and infiltration of the above-mentioned inducer active principle on plant leaf surface, can be improved
The transfer for stating active principle improves the effective rate of utilization of inducer.
The type of surfactant and sustained release agent is selected to inducer active ingredient amino-oligosaccharide, phosphite and phosphoric acid
The application effect of salt has tremendous influence, suitable surfactant and sustained release agent to can further improve derivant to cucumber samping off
With the control effect of damping-off.
Preferably, the surfactant is Tween-80, the sustained release agent is fulvic acid potassium.
Wherein, Tween-80 is also known as polyoxyethylene sorbitan monoleate or polyoxyethylene sorbitan monolaurate 80, has more
Strong surface-active ability.Potassium fulvate is a kind of pure natural minerals activity potassium element fertilizer, and potassium fulvate includes micro-, dilute
It is each caused by due to lacking element to avoid crop for the multiple nutritional components such as earth elements, plant growth regulator, viral inhibitors
The generation of kind physiological disturbance, keeps the more vigorous leaf color of crop plant type more dark green, lodging tolerance is stronger.It is found through experiment that with Huang
Humic acid potassium can further improve the control effect of derivant, and energy as sustained release agent and the cooperation of amino-oligosaccharide and phosphite
Enough improve crop yield.
Preferably, the plant immune inducer, by total weight for 1kg in terms of, each group distribution ratio is：
It is further preferred that the plant immune inducer, by total weight for 1kg in terms of, each group distribution ratio is：
The present invention also provides a kind of methods of prevention cucumber samping off and damping-off, include the following steps：
(1) after planting, into the soil of sowing, inducer is immunized in irrigating plant to cucumber；
(2) after cucumber seedling grows rough leaf, exempt to spray on plants in the soil of the seedling and seedling periphery
Epidemic disease inducer；
In step (1) and step (2), the active ingredient of percentage, the plant immune inducer includes：
0.001~0.025% amino-oligosaccharide, 0.01~0.25% phosphite and 0.01~0.25% phosphate.
The conventional application method of plant immune inducer be seed soaking, seed dressing or foliage-spray, can promote plant absorption,
It utilizes；And in the present invention in addition to carrying out foliage-spray to Leaf of Cucumber Seedling, also into the soil of sowing and seedling periphery
Inducer is immunized in irrigating plant in soil, experiments have shown that, cucumber samping off can preferably be prevented using the above method and stood withered
Disease.
Preferably, in step (1) and step (2), the amount of application of the plant immune inducer is 25~35kg/ mus；
Wherein, in terms of by total weight for 1kg, each group distribution ratio of the plant immune inducer is：
Specifically, in step (1), the pouring number of the plant immune inducer is 1 time；In step (2), the plant
The frequency that sprays of immune inducer is：It was sprayed every 7 days 1 time, totally 3 times.
Although above-mentioned plant immune inducer also can be used in preventing other diseases of cucumber, experiments have shown that, the plant
Immune inducer is very good for the samping off and damping-off preventive effect of cucumber, then coordinates suitable amounts of components and application side
Formula, it is splendid for the preventive effect of cucumber samping off and damping-off.
Compared with prior art, the invention has the advantages that：
(1) amino-oligosaccharide, phosphite and phosphate are combined by the present invention, the immune inducer each component of acquisition
Mixed proportion is reasonable, not only good to the control effect of cucumber samping off and cucumber rhizoctonia rot disease, but also can improve cucumber
Emergence rate, planting percent and Seedling Quality.
(2) present invention further improves inducer to cucumber samping off and Huang using fulvic acid potassium as sustained release agent
The control effect of melon damping-off disease, and promote the raising of the emergence rate, planting percent and Seedling Quality of cucumber.
(3) plant immune inducer is administered by the present invention by pouring and spraying two ways, and plant immune is made to lure
Anti-agent is further improved the control effect of cucumber samping off and cucumber rhizoctonia rot.
Specific implementation mode
Present invention will be further explained and explained with reference to specific examples below.
In the following example the preparation method of plant immune inducer use conventional pesticide preparation method, i.e., by each component in
In water after mixed dissolution, obtains cucumber of the present invention and inducer is immunized.
One, test material
Cucumber variety is ' Tianjin spring No. 4 '.
Two, inducer method of application is immunized
It tests and is carried out in the plant protection clay fertilizer master station Binjiang proving ground of Hangzhou.Seedling medium is vegetable garden soil：Peat：Charcoal health
Ash (12:15:23), and appropriate composite fertilizer is added.
9 processing of experiment setting, wherein comparative example 1 (control 1) is that clear water solution compares, and comparative example 2 (control 2) is more
Bacterium spirit, each handles 3 repetitions, is each repeated as 1 cell, per 100 seeds of cell.
The specific method of administration of immune inducer is：(Examples 1 to 7 and comparative example 1~2 are all made of the method for administration)
(1) cucumber seeds are sowed into experimental plot, the blinding on seed, then pour cucumber into soil and inducer is immunized
To ground moistening, emergence is waited for；Before emergence, cucumber is immunized inducer and pours altogether once, and amount of application is 30kg/ mus, and (cucumber is immune
The effective concentration of each component is as shown in following Examples 1 to 7 and comparative example 1~2 in inducer)；
(2) after cucumber seedling grows rough leaf, exempt to spray on plants in the soil of the seedling and seedling periphery
Epidemic disease inducer, spraying frequency is：It was sprayed every 7 days 1 time, totally 3 times；Every time spray cucumber be immunized inducer amount of application be
30kg/ mus (cucumber is immunized the same step of effective concentration (1) of each component in inducer, and such as following Examples 1 to 7 and comparative example 1~
Shown in 2).
After immune inducer, the incidence of plant strain growth situation and sprout term disease is observed.
Embodiment 1
A kind of plant immune inducer, in terms of 1 kilogram of total weight, the dosage of each component is：
Embodiment 2
A kind of plant immune inducer, in terms of 1 kilogram of total weight, the dosage of each component is：
Embodiment 3
A kind of plant immune inducer, in terms of 1 kilogram of total weight, the dosage of each component is：
Embodiment 4
A kind of plant immune inducer, in terms of 1 kilogram of total weight, the dosage of each component is：
Embodiment 5
A kind of plant immune inducer, in terms of 1 kilogram of total weight, the dosage of each component is：
Embodiment 6
A kind of plant immune inducer, in terms of 1 kilogram of total weight, the dosage of each component is：
Embodiment 7
A kind of plant immune inducer, in terms of 1 kilogram of total weight, the dosage of each component is：
Comparative example 1
A kind of pesticide, in terms of 1 kilogram of total weight, the dosage of each component is：
Surfactant 0.1g；
Surplus is water.
Comparative example 2
A kind of pesticide, in terms of 1 kilogram of total weight, the dosage of each component is：
Carbendazim 0.8g；
Surplus is water.
To treated the cucumber seedling disease investigation that a situation arises of above-described embodiment and comparative example, and measure cucumber emergence
Rate, planting percent and Seedling Quality, as a result as shown in table 1, table 2, table 3.
The different immune inducers of table 1 handle the influence to cucumber at seedling stage disease incidence
The different inducers of table 2 handle the influence to cucumber emergence rate and planting percent
The different inducers of table 3 handle the influence to Cucumber Seedling Quality
| Number | Plant height (cm) | Main root grows (cm) | Root number (a) | Leaf grows (cm) | Leaf width (cm) |
| Embodiment 1 | 4.54±0.30 | 4.91±0.37 | 9.33±0.84 | 3.63±0.08 | 1.17±0.03 |
| Embodiment 2 | 3.65±0.32 | 4.62±0.05 | 9.22±0.59 | 3.43±0.13 | 1.07±0.02 |
| Embodiment 3 | 3.72±0.38 | 5.51±0.30 | 10.89±0.95 | 3.44±0.18 | 1.03±0.08 |
| Embodiment 4 | 3.69±0.07 | 5.54±0.34 | 10.22±0.40 | 3.53±0.15 | 1.10±0.02 |
| Embodiment 5 | 3.71±0.23 | 4.98±0.35 | 8.33±1.02 | 3.41±0.11 | 0.94±0.01 |
| Embodiment 6 | 3.09±0.09 | 3.89±0.58 | 8.33±1.17 | 3.26±0.07 | 0.91±0.04 |
| Embodiment 7 | 3.27±0.21 | 3.53±0.07 | 5.56±0.68 | 3.24±0.19 | 0.94±0.05 |
| Comparative example 1 | 2.47±0.42 | 3.86±0.55 | 7.56±0.48 | 3.17±0.28 | 0.88±0.11 |
| Comparative example 2 | 3.01±0.05 | 4.11±0.73 | 9.11±1.22 | 3.16±0.06 | 0.93±0.05 |
As it can be seen from table 1 handling phase with spray clear water processing (compareing 1) in comparative example 1 and 2 conventional pesticide of comparative example
Than all embodiments can reduce the incidence of samping off.For damping-off, compared with comparative example 1 and comparative example 2, except real
It is higher outer to apply 7 incidence of example, the incidence that inducer embodiment can significantly reduce damping-off is immunized in remaining.
From table 2 it can be seen that compared with spray clear water processing (control 1) in comparative example 1, all embodiments can improve Huang
Melon emergence rate, and compared with the processing of 2 conventional pesticide of comparative example, the inducer leaf emergence only in embodiment 1,2,3 and 4 increases
Add, wherein embodiment 1,3 emergence rate highests, remaining embodiment emergence rate is close or is less than comparative example 2.
For planting percent, compared with comparative example 1, in addition to 7 planting percent of embodiment is relatively low, the processing of remaining embodiment can carry
High cucumber planting percent, and compared with the processing of 2 conventional pesticide of comparative example, in addition to embodiment 5,6,7, remaining embodiment planting percent increases
Add, wherein 1 planting percent highest of embodiment.
From table 3 it can be seen that compared with comparative example 1 and comparative example 2, all embodiments can increase cucumber seedling plant height,
Wherein 1 highest of embodiment, 2 cucumber seedling plant height of comparative example are higher than comparative example 1.In addition to embodiment 7, remaining embodiment cucumber children
Seedling main root grow and root number is above comparative example 1, wherein 4 main root longest of embodiment, 3 root number of embodiment is most, and with comparison
Example 2 is compared, except outside embodiment 6,7, it is long that the processing of remaining embodiment improves cucumber seedling main root.And root number removes embodiment 6,7
Outside, remaining embodiment processing is above comparative example 2.
In addition, compared with comparative example 1, all embodiments can increase cucumber seedling leaf length and leaf width, and wherein embodiment 1 is yellow
Melon seedling leaf is grown and leaf width highest.Compared with comparative example 2, it is long that all embodiments can increase cucumber seedling leaf, and leaf width is except real
It applies outside example 6, the processing of remaining embodiment is higher.
For embodiment 1,2,3 and 4 test results, amino-oligosaccharide is mixed with phosphite to use tool than individually a kind of
It becomes apparent from and disease-resistant cucumber, emergence performance improvement and Seedling Quality is promoted to improve, show that amino-oligosaccharide is mixed with phosphite
With apparent synergistic effect.
Claims (4)
1. a kind of method of prevention cucumber samping off and damping-off, which is characterized in that include the following steps：
（1）After planting, into the soil of sowing, inducer is immunized in irrigating plant to cucumber；
（2）After cucumber seedling grows rough leaf, lured to spray on plants in the soil of the seedling and seedling periphery is immune
Anti-agent；
Step（1）And step（2）In, the active ingredient of percentage, the plant immune inducer is：0.001~
0.025% amino-oligosaccharide, 0.01 ~ 0.25% phosphite, 0.01 ~ 0.25% phosphate, 0.001 ~ 0.02% surface
Activating agent and 0.001 ~ 0.02% sustained release agent；
The phosphite is potassium dihydrogen phosphite；The phosphate is potassium dihydrogen phosphate；The surfactant is Tween-
80；The sustained release agent is fulvic acid potassium.
2. the method as described in claim 1, which is characterized in that percentage, the plant immune inducer have
Imitating ingredient is：0.005 ~ 0.012% amino-oligosaccharide, 0.05 ~ 0.12% phosphite, 0.05 ~ 0.12% phosphate,
0.001 ~ 0.02% surfactant and 0.001 ~ 0.02% sustained release agent.
3. the method as described in claim 1, which is characterized in that by total weight be 1 kg in terms of, the plant immune inducer
Each group distribution ratio is：
Tween-80 0.05~0.12 g；
0.25 ~ 1.20g of potassium dihydrogen phosphite；
0.25 ~ 1.20g of potassium dihydrogen phosphate；
0.05 ~ 0.12 g of amino-oligosaccharide；
0.02 ~ 0.12 g of fulvic acid potassium；
Surplus is water.
4. the method as described in claim 1, which is characterized in that step（1）In, the pouring number of the plant immune inducer
It is 1 time；Step（2）In, the frequency that sprays of the plant immune inducer is：It was sprayed every 7 days 1 time, totally 3 times.
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