CN102388918B - Composite of toxic fluoride phosphate and plant growth regulator - Google Patents
Composite of toxic fluoride phosphate and plant growth regulator Download PDFInfo
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Abstract
The invention provides a formulation composite comprising toxic fluoride phosphate and at least one growth regulator. According to the weight part, the toxic fluoride phosphate accounts for 10%-80% of the formulation composite, the growth regulator accounts for 0.0001%-2% of the formulation composite, the formulation composite also comprises an assistant, an surface active agent, a solvent and a carrier accepted by agriculture, and the formulation composite can be prepared into wettable powder, suspending agent, missible oil, microemulsion, emulsion in water, water dispersible granule, dry suspending agent, seed coating agent and other agents. The formulation composite can be used for preventing and curing tobacco mosaic virus, cucumber mosaic virus, and various virus diseases caused by viruses on rice, such as tobacco mosaic, cucumber mosaic, tomato virus disease, pepper virus disease, rice virus disease and the like and achieves the effects of improving efficacy, treating in a compatible manner, delaying medicine resistance, reducing the using amount of toxic fluoride phosphate and the like.
Description
Technical field
The present invention relates to the compound pesticide composition field, more particularly relate to the complex composition of malicious fluorine phosphorus and plant growth regulator, and the preparation of the various formulations of said composition and application technology.
Background technology
In recent years various crop virosis present serious development trend, except tobacco mosaic, cucumber mosaic and tomato virus disease that tradition comparatively generally occurs.The rice stripe leaf is withered, rice black-streaked dwarf, southern rice black-streaked dwarf, and morbidity is exponential increase.Special southern rice black-streaked dwarf first after Yangxi County, Guangdong finds, has generation in Guangdong since calendar year 2001 year after year; After 2003, Hainan also occurs year after year; 2008 in Guangdong, the ground such as Guangxi, Hainan, Hunan, Jiangxi, Anhui all finds, only some areas are injured seriously.2009, south China 9 provinces and regions generations, approximately 2,000,000 mu of injured areas, the part field is lost and is received, approximately 70,000 tons of losses.2010, this disease seriously occured in China 11 provinces, approximately 1,800 ten thousand mu of areas.Infecting of this disease can cause serious production loss, and therefore even large stretch of total crop failure is controlled the expansion of disease, and guaranteed that output is very important and urgent.
Poison fluorine phosphorus is the novel Antiphytoviral medicament of fluorine-containing amino phosphonate ester of novel structure, can by activating tobacco bigcatkin willow acid signal conduction path, improve the salicylic content of signaling molecule, thereby promote the expression of downstream pathogenesis-related proteins; Obtain anti-virus ability by evoking tobacco PAL, POD, SOD Defense Enzyme Activities; Reduce virus to host's invasion by assembling the TMV particle.The registered control for tobacco virus of poison fluorine phosphorus because it has good control efficiency to cucumber, tomato virus disease, is just being applied for using on the crops such as cucumber, tomato at present., production technology environmentally friendly due to this medicament is simple, three-waste free pollution, the more commercially available medicament of production cost are low, simultaneously also with improving the plant disease-resistant ability, therefore this medicament is expected to the larger effect of performance in the integrated control viroses of plant, therefore malicious fluorine phosphorus is more promising bactericide new varieties, has application value.At present, the malicious fluorine phosphorus preparation of registration only has 30% wetting powder, and former Guizhou University patented product now turns Guangxi Tianyuan Biochemical Co., Ltd. and owns.Patent (application number: 200510003041.7, the patent No.: being ZL200510003041.7) preparation of malicious fluorine phosphorus single-activity composition, is not composition.Yet, a kind of appearance of new drug, if long-term single use all inevitably produces resistance, antivirotic is no exception.Therefore, by composite channel, adopt malicious fluorine phosphorus and other growth regulator composite, composition will be the effective way of resisting resistance, delaying drug resistance development.
In addition, the pathogenesis of virus disease is very complicated, and the eubolism that can upset plant cell after morbidity is movable, reduces the adjusting immune system of plant self, and the g and D of plant is destroyed.Therefore, use the physiological metabolism balance that plant growth regulator can in time be regulated plant, help plant self to improve immunity, enhance disease resistance.Therefore, the present invention will provide a cover diseases prevention and improve the medicament of plant autoimmunity ability, and related plant growth regulator has brassin lactones, compound sodium nitrophenolate, diethyl aminoethyl hexanoate and gibberellin etc.
Brassin lactones: 2 α, 3 α, 22s, 23s-tetrahydroxy-24R-ethyl-β-Gao-7-oxa-5 alpha-courage steroid-6-ketone, chemical molecular formula: C
29H
50O
6, molecular weight: 494, its chemical constitution is as follows:
Brassin lactones one class plant endogenous hormones, the enhancing crop is cold-resistant, waterlogging-resistant, the poisoning ability is conciliate in drought resisting, can strengthen photosynthesis, strengthens vegetation growth of plant, promote cell division, reproductive growth effect.
Compound sodium nitrophenolate is the compound of 5-nitroguaiacol sodium salt, sodium onitrophenol and Sodium p-nitrophenoxide, the energy rapid permeability is in plant corpus, flow with the protoplasm that promotes cell, accelerate plant rooting speed, all there is the different facilitation of degree the developmental stage such as plant rooting, growth, reproduction and result.This product can be processed with modes such as foliage spray, seed soaking, seedbed perfusion and bud spreadings.Because they are different from plant hormone, begin all can use to any period between results at plant seeding.
Diethyl aminoethyl hexanoate has the high energy plant growth regulator of wide spectrum and breakthrough effect.It can improve the activity of plant peroxidases and nitrate reductase, improves chlorophyllous content and accelerates photosynthetic speed, promotes division and the elongation of plant cell, promotes the growth of root system, the balance of nutrient in control agent.
Gibberellin: 2,4a, 7-trihydroxy-1-methyl-8-methylene red mould-3-alkene-1,10-dicarboxylic acids-Isosorbide-5-Nitrae a-lactone, its chemical constitution is as follows:
Gibberellin is the broad spectrum activity plant growth regulator, but the important hormone of Promoting plant growth, growth is the antagonist of the growth inhibitors such as paclobutrazol, chlormequat.This medicament can promote cell division, and stem elongation, blade expansion, parthenocarpy, fruit growth are broken seed dormancy, change female, male flower ratio, affect flowering time, reduces coming off of flower, fruit.External source gibberellin enters in plant corpus, has the same physiological function of endogenous hormones gibberellin.Gibberellin mainly in blade, branch, flower, seed or fruit entered into the plant body, worked by the position that then is transmitted to active growth.
Based on above-mentioned analysis, we are mixed with conditioning agent with malicious fluorine phosphorus, and on the one hand malicious fluorine phosphorus suppresses virus, induces the plant disease resistance, on the other hand, growth regulator is in time regulated the physiological metabolism balance of plant, improves the growing way of plant, enhances disease resistance, thereby suppressing the virus disease symptom shows, significantly improve preventive effect, finally play and protect the effect of producing volume increase, have extraordinary synergistic function.The present invention namely for the demand of Different Crop, combines malicious fluorine phosphorus with different types of conditioning agent, regulate comprehensively and improve the ntiviral characteristic of crop, and controls virus, thereby reaches the effect of prevention and control virus disease preferably.
Summary of the invention
One of purpose of the present invention is to provide a kind of toxic fluorine phosphorus and at least a growth regulator as the antiviral composition of active component, and by composite, regulating plant physiology and metabolism, induce the plant resistance comprehensively, and prevention and control virus is protected to greatest extent and produced volume increase.
Two of purpose of the present invention is the combinations with this active ingredient, adds suitable auxiliary agent, thinner, surfactant, solvent and carrier etc. to make operable formulation product.
The present invention is based on the complex composition of malicious fluorine phosphorus and growth regulator, wherein malicious fluorine phosphorus accounts for the 10%-80% of complex composition by weight, and growth regulator accounts for the 0.0001%-2% of complex composition, except active component.Preferably, malicious fluorine phosphorus accounts for the 20%-70% of composition by weight, and growth regulator accounts for the 0.0005%-2% of composition.
This composition also comprises auxiliary agent, thinner, surfactant, solvent and carrier.
Described auxiliary agent and surfactant are one or more in dispersing agent NNO (sodium metnylene bis-naphthalene sulfonate), lauryl sodium sulfate, dispersing agent MF, sodium lignin sulfonate, agriculture breast 600#, fatty alcohol-polyoxyethylene ether, naphthalene sulfonic acid formaldehyde condensation polymer, ammonium sulfate, emulsifier 603#, calcium dodecyl benzene sulfonate (500#); Described solvent or cosolvent are isopropyl alcohol, dimethyl formamide, cyclohexanone, ethyl acetate, dimethylbenzene etc.; Described carrier is one or more in white carbon, kaolin, water, potter's clay.
Described growth regulator is selected from least a in following compounds: brassin lactones, compound sodium nitrophenolate, diethyl aminoethyl hexanoate (DA-6), the basic element of cell division, heteroauxin, Nafusaku, gibberellin, triacontanol, root-inducing powder, dragon flying around No., CPPU.
The formulation of described complex composition is wetting powder, suspending agent, missible oil, microemulsion, aqueous emulsion, water dispersible granules, dried suspension emulsion, plants the clothing agent.
The compound pesticide composition that the poisoning fluorine phosphorus of the present invention forms in certain proportion with growth regulator respectively, the prevention that shows and delay virus disease, and protect and produce the volume increase aspect and be better than each component and use separately.
A large amount of research and practice show the effective prevention and control of virus disease, the most importantly effect of two aspects, and one is infecting and diffusivity of attenuated virus particle, another improves the immunity of crop self, strong sprout, short long, guarantor's product.Poison fluorine phosphorus is composite with relevant conditioning agent, can so that regulating crop growth complements each other with suppressing virus, control more timely and effectively virus disease.
The present invention carries out mixture preparation by malicious fluorine phosphorus and growth regulator, mainly take Virus Diseases of Rice, tobacco mosaic, cucumber mosaic, tomato virus disease as research object, is used for paddy field, vega, vegetable fields etc., is pesticidal preparations.
The present invention carries out composite mix preparation and application thereof by malicious fluorine phosphorus and conditioning agent, and its advantage and good effect are:
1, more efficient prevention and control virus disease: by selecting different conditioning agents for Different Crop, regulating plant physiology makes malicious fluorine phosphorus to do mutually with the host, thereby the resistance of inducing to greatest extent crop suppresses the expansion of virus in plant corpus.
2. protect to greatest extent and produce and volume increase: malicious fluorine phosphorus and conditioning agent are composite, when malicious fluorine phosphorus suppresses virus, and conditioning agent regulating plant internal metabolism balance, the regulation and control plant physiology, Symptoms alleviates, production loss reduces thereby make.
3. filled up the blank of pesticide market, response national policy, the demand of satisfying the market.At present domestic medicament kind of preventing and treating virus disease seldom, and effect is also undesirable, along with the generation of various virus diseases in recent years increases gradually, especially the virus disease on paddy rice, many rice fields even face the danger of total crop failure, and the peasant is better in the urgent need to preventive effect, the medicament of reasonable price comes the prevention and control virus disease.Poison fluorine phosphorus is independently to formulate product, the policy of respective country autonomous innovation.
Embodiment
The invention will be further described below by embodiment.It should be understood that the described preparation method of the embodiment of the present invention is only used for illustrating the present invention, rather than limitation of the present invention, the simple modifications to preparation method of the present invention under design prerequisite of the present invention all belongs to the scope of protection of present invention.In all formulas, percentage all is weight percentage.
Embodiment 1:
40% malicious fluorine phosphorus basic element of cell division wetting powder proportioning
Various compositions are mixed, namely obtain product through air-flow crushing by 325 mesh sieves.
Embodiment 2:
30% malicious fluorine phosphorus compound sodium nitrophenolate suspending agent proportioning
Various compositions are mixed, be ground to the active ingredient particle diameter less than 4 μ m in sand mill.Sodium lignin sulfonate, fatty alcohol-polyoxyethylene ether, agriculture breast 600# add the preparation that helps formulation, can keep the stability of formulation, help storage, transportation, guarantee drug effect.
Embodiment 3:
30% malicious fluorine phosphorus diethyl aminoethyl hexanoate suspending agent proportioning
Various compositions are mixed, be ground to the active ingredient particle diameter less than 4 μ m in sand mill.
Embodiment 4:
30% malicious fluorine phosphorus Nafusaku suspending agent proportioning
Various compositions are mixed, be ground to the active ingredient particle diameter less than 4 μ m in sand mill.
Embodiment 5:
70% malicious fluorine phosphorus heteroauxin suspending agent proportioning
Various compositions are mixed, be ground to the active ingredient particle diameter less than 4 μ m in sand mill.
Embodiment 6:
60% malicious fluorine phosphorus brassin lactones water dispersible granules proportioning
Said mixture is evenly mixed, and air-flow crushing adds suitable quantity of water to mediate, and the mixture pelleting of gained, is drying to obtain product.Adding of the auxiliary agents such as naphthalene sulfonic acid formaldehyde condensation polymer, lauryl sodium sulfate, ammonium sulfate, potter's clay, both made providing safeguard for formulation, can make again product keep good dispersiveness, relatively good to the tack on target surface.
Embodiment 7:
30% malicious fluorine phosphorus basic element of cell division water dispersible granules proportioning
Said mixture is evenly mixed, and air-flow crushing adds suitable quantity of water to mediate, and the mixture pelleting of gained, is drying to obtain product.
Embodiment 8:
60% malicious fluorine phosphorus gibberellin water dispersible granules proportioning
Said mixture is evenly mixed, and air-flow crushing adds suitable quantity of water to mediate, and the mixture pelleting of gained, is drying to obtain product.
Embodiment 9:
40% malicious fluorine phosphorus diethyl aminoethyl hexanoate suspending agent proportioning
Various compositions are mixed, be ground to the active ingredient particle diameter less than 4 μ m in sand mill.
Embodiment 10:
35% malicious fluorine phosphorus triacontanol wetting powder proportioning
Various compositions are mixed, namely obtain product through air-flow crushing by 325 mesh sieves.
Embodiment 11:
30% malicious fluorine phosphorus CPPU suspending agent proportioning
Various compositions are mixed, be ground to the active ingredient particle diameter less than 4 μ m in sand mill.
Embodiment 12:
55% malicious fluorine phosphorus root-inducing powder suspending agent proportioning
Various compositions are mixed, be ground to the active ingredient particle diameter less than 4 μ m in sand mill.
Embodiment 13:
10.01% malicious fluorine phosphorus brassin lactones microemulsion proportioning
First with dissolving former medicine dissolve complete, then add wetting dispersing agent to mix and namely get oil phase, then add entry and mix and namely get transparent homogeneous liquid.
Embodiment 14:
Aqueous emulsion proportioning of 20% malicious fluorine phosphorus dragon flying around
After first using solvent former medicine dissolve complete, and wetting dispersing agent, water adds together and carries out shear-mixed in clipper, namely gets and evenly namely gets the emulsus homogeneous liquid.
For clear and definite malicious fluorine phosphorus alone with conditioning agent and when mixed to the control efficiency of Virus Diseases of Rice, tobacco mosaic, the applicant had carried out a large amount of field trials in different regions at home in 2010.
Biologically active embodiment
Stripe disease field efficacy embodiment (Anqing):
Process 1:30% poison fluorine phosphorus compound sodium nitrophenolate SC+10% Nitenpyram EC
Process 2:30% poison fluorine phosphorus WP+10% Nitenpyram EC
Process 3:1.8% compound sodium nitrophenolate AS+10% Nitenpyram EC
Process 4: blank
Test method: direct seading rice, in early tillering stage dispenser for the first time, interval 7d dispenser again, dispenser is 2 times altogether, and each processes 0.5-1 mu.
Investigation method: 150 clumps of random searchings, disease index is recorded in classification, calculates preventive effect.After maturation, gather at random 30 clumps, threshing is dried, and weighs, and calculates the rate of growth of comparing with CK.
Experimental result: as shown in Table 1, in early tillering stage prevention of water rice stripe, on preventive effect, malicious fluorine phosphorus+conditioning agent effect be better than malicious fluorine phosphorus or conditioning agent alone, on output, effect is more obvious.
Table 1 early tillering stage is pesticide supplying effect for the first time
Annotate: except blank, it is that the blocking-up plant hopper is propagated malicious source that each processing all adds 10% Nitenpyram 60mL/ mu, purpose, to improving preventive effect.
The sick field efficacy embodiment (Shangrao, Jiangxi) of southern rice black-streaked dwarf:
Process 1:30% poison fluorine phosphorus compound sodium nitrophenolate SC+10% Nitenpyram EC
Process 2:30% poison fluorine phosphorus WP+10% Nitenpyram EC
Process 3:1.8% compound sodium nitrophenolate AS+10% Nitenpyram EC
Process 4: blank
Test method: in the semilate rice land for growing field crops initial stage, i.e. rice tillering initial stage dispenser for the first time, interval 7d dispenser again, dispenser is 2 times altogether, and each processes 0.8-1 mu.
Investigation method: 200 clumps of random searchings, disease index is recorded in classification, calculates preventive effect.
Experimental result: sick at land for growing field crops initial stage prevention southern rice black-streaked dwarf as shown in Table 2, on preventive effect, malicious fluorine phosphorus+conditioning agent effect be better than malicious fluorine phosphorus or conditioning agent alone.
The table 2 semilate rice early tillering stage is pesticide supplying effect for the first time
Annotate: except blank, it is that the blocking-up plant hopper is propagated malicious source that each processing all adds 10% Nitenpyram 50mL/ mu, purpose, to improving preventive effect.
The sick field efficacy embodiment (Pingxiang Jiangxi) of southern rice black-streaked dwarf:
Process 1:30% poison fluorine phosphorus DA-6SC+25% Buprofezin WP
Process 2:30% poison fluorine phosphorus WP+25% Buprofezin WP
Process 3:1.6%DA-6AS+25% Buprofezin WP
Process 4: blank
Test method: in nursery period, namely 2 one heart stages of leaf of rice shoot, begin dispenser for the first time, interval 7d dispenser again, and dispenser is 2 times altogether, and each processes 10 square metres.
Investigation method: investigate all strong seedlings and the sick seedling number of 10 square metres, and carry out the classification investigation according to conventional grade scale, calculate disease index, preventive effect.
Experimental result: as shown in Table 3, pre-gas defence fluorine phosphorus of rice seedling bed phase+conditioning agent effect be better than malicious fluorine phosphorus or conditioning agent alone.
Table 3 semilate rice nursery period, is pesticide supplying effect for the first time
Annotate: except blank, it is that the blocking-up plant hopper is propagated malicious source that each processing all adds 25% Buprofezin 40mL/ mu, purpose, to improving preventive effect.
The sick field efficacy embodiment (Ganzhou) of southern rice black-streaked dwarf:
Process 1:30% poison fluorine phosphorus Nafusaku SC+10% Nitenpyram
Process 2:30% poison fluorine phosphorus WP+10% Nitenpyram
Process 3:2.85% Nafusaku AS+10% Nitenpyram
Process 4: blank
Test method: in the middle of tillering of the dispenser for the first time of late rice field rice, interval 7d dispenser again, dispenser is 2 times altogether, and each processes 0.4-0.6 mu.
Investigation method: 150 clumps of random searchings, disease index is recorded in classification, calculates preventive effect.
Experimental result: as shown in Table 4, in land for growing field crops initial stage prevention of water rice black streak dwarf, on preventive effect, malicious fluorine phosphorus+conditioning agent effect be better than malicious fluorine phosphorus or conditioning agent alone.
Table 4 late rice pesticide supplying effect middle of tillering
Annotate: except blank, it is that the blocking-up plant hopper is propagated malicious source that each processing all adds 10% Nitenpyram 50mL/ mu, purpose, to improving preventive effect.
Tobacco mosaic field efficacy embodiment (Ningnan, Sichuan):
Process SC of 1:30% poison fluorine phosphorus dragon flying around
Process 2:30% poison fluorine phosphorus WP
Process ME of 3:5% dragon flying around
Process 4: blank
Test method: dispenser for the first time after tobacco transplant 15-20d, interval 7d dispenser again, dispenser is three times altogether.
Investigation method: respectively at 7d, 14d, 21d investigation after three medicines, according to data correlation study standard grading, the statistics disease index calculates preventive effect.
Experimental result: as shown in Table 5, WP of 30% malicious fluorine phosphorus dragon flying around, effect is better than 30% malicious fluorine phosphorus WP and ME of 5% dragon flying around, and namely composite control tobacco virus successful of malicious fluorine phosphorus and dragon flying around is higher than the independent use of these two compositions.
The effect of table 5 different disposal control tobacco virus
The sick field control effectiveness test one of southern rice black-streaked dwarf:
Process 1:10.01% poison fluorine phosphorus brassin lactones ME+10% Nitenpyram EC
Process 2:30% poison fluorine phosphorus WP+10% Nitenpyram EC
Process 3:0.1% brassin lactones WP+10% Nitenpyram EC
Process 4: blank
Test method: dispenser for the first time after rice transplanting 10-15d, interval 7d dispenser again, dispenser twice altogether, each processes 90 square metres.
Investigation method: respectively at 7d, 14d, 21d investigation after twice medicine, adopts 5 samplings, 200 clumps of every residential quarter investigation, according to related data investigation standard grading, the statistics disease index, the calculating preventive effect the results are shown in Table 6.
The effect of table 6 different disposal control southern rice black-streaked dwarf disease
Annotate: except blank, it is that the blocking-up plant hopper is propagated malicious source that each processing all adds 10% Nitenpyram 50mL/ mu, purpose, to improving preventive effect.
Experimental result: as shown in Table 6,10.01% malicious fluorine phosphorus brassin lactones microemulsion, effect is better than 30% malicious fluorine phosphorus WP and 0.1% brassin lactones WP, and namely the sick successful of the composite control southern rice black-streaked dwarf of malicious fluorine phosphorus and brassin lactones is better than these two compositions and uses separately.
The sick field control effectiveness test two of southern rice black-streaked dwarf:
Process EW+10% Nitenpyram EC of 1:20.03% poison fluorine phosphorus dragon flying around
Process 2:30% poison fluorine phosphorus WP+10% Nitenpyram EC
Process EW+10% Nitenpyram EC of 3:5% dragon flying around
Process 4: blank
Test method: dispenser for the first time after rice transplanting 15-20d, interval 7d dispenser again, dispenser twice altogether, each processes 90 square metres.
Investigation method: respectively at 7d, 14d, 21d investigation after twice medicine, adopts 5 samplings, 200 clumps of every residential quarter investigation, according to related data investigation standard grading, the statistics disease index, the calculating preventive effect the results are shown in Table 7.
The effect of table 7 different disposal control southern rice black-streaked dwarf disease
Annotate: except blank, it is that the blocking-up plant hopper is propagated malicious source that each processing all adds 10% Nitenpyram 50mL/ mu, purpose, to improving preventive effect.
Experimental result: as shown in Table 7, EW of 20.03% malicious fluorine phosphorus dragon flying around, effect is better than 30% malicious fluorine phosphorus WP and EW of 5% dragon flying around, and namely the sick successful of the composite control southern rice black-streaked dwarf of malicious fluorine phosphorus and dragon flying around No. is better than these two compositions and uses separately.
All relate to the alone test statistics result of comparing of composite with conditioning agent and the malicious fluorine phosphorus of malicious fluorine phosphorus (between wherein processing, the preventive effect absolute difference is effective greater than 2%, otherwise effect is suitable):
Test site: Guangxi, Jiangxi, Anhui, Sichuan
Test period: 2010-04 to 2010-11
Trial crops and disease: stripe disease, southern rice black-streaked dwarf disease, tobacco mosaic, tomato virus disease etc.
Test is processed: respectively malicious fluorine phosphorus being added different conditioning agents, design simultaneously malicious fluorine phosphorus single dose and foliage fertilizer single dose and process, is all dispenser 2-3 times.
Investigation method: 7-21d investigation after dispenser, adopt different disease index stage divisions to carry out classification investigation, the yield effect that subtrace is last according to different crop pests.Experimental result statistics sees Table 8 and table 9.
The alone effect of the preparation that the malicious fluorine phosphorus of table 8 and conditioning agent are composite and malicious fluorine phosphorus relatively
The alone effect of the preparation that the malicious fluorine phosphorus of table 9 and conditioning agent are composite and conditioning agent relatively
Can find out from a large amount of fields, malicious fluorine phosphorus and the composite use of conditioning agent can better suppress virus disease, regulate crop metabolism and physiological equilibrium, improve resistance, thereby prevent and delay generation or the expansion of disease, retrieve to greatest extent production loss.
Toxicity Determination:
Toxicity Determination embodiment 1:
Indoor pot tobacco susceptible variety plant is to the 4-5 leaf phase, and with the isopyknic viral juice frictional inoculation of liquid mixing of different proportioning variable concentrations, every processing 3 strains, 3 leaves of often selecting good strains in the field for seed repeat 4 times, and 3-4 days " Invest, Then Investigate " withered spot numbers calculate inhibiting rate, use EC
50Value is calculated co-toxicity coefficient, and measurement result sees Table 10.
Virulence regression equation and the co-toxicity coefficient of table 10 reagent agent to tobacco virus
By data in table 10 as seen, malicious fluorine phosphorus and the basic element of cell division are composite in above ratio, and co-toxicity coefficient antagonism namely do not occur all greater than 80.Co-toxicity coefficient was greater than 120 when wherein ratio was composite with 39.96: 0.04, illustrated that this ratio is composite tobacco mosaic is had synergistic effect.
Toxicity Determination embodiment 2:
Indoor pot paddy rice susceptible variety when rice shoot grows to 1 leaf 1 heart～2 leaf 1 heart, is carried out inoculated identification.Every alms bowl cover is with translucent cover, and gauze sealing in top calculates by 5 small brown rice planthoppers of every seedling the worm amount that needs, access small brown rice planthopper in 2～3 age (be with malicious rate approximately 40%).Expelling parasite every day 2 times makes tested rice seedling evenly be subjected to poison.The medicament for preparing variable concentrations is evenly sprayed on for the examination seedling, and every processing 50 strains repeat for 4 times, and after medicine, classification in the 14 days investigation state of an illness, calculate preventive effect, uses EC
50Value is calculated co-toxicity coefficient, and measurement result sees Table 11.
Virulence regression equation and the co-toxicity coefficient of table 11 reagent agent to stripe disease
By data in table 11 as seen, malicious fluorine phosphorus and compound sodium nitrophenolate are composite in above ratio, and co-toxicity coefficient antagonism namely do not occur all greater than 80.And co-toxicity coefficient is all greater than 120 so that ratio was composite in 29.7: 0.3,29.5: 0.5,29.3: 0.7, illustrates that this ratio is composite stripe disease is all had synergistic effect.Wherein 29.5: 0.5 composite co-toxicity coefficients are maximum.
Toxicity Determination embodiment 3:
Indoor pot tobacco susceptible variety plant is to the 4-5 leaf phase, and with the isopyknic viral juice frictional inoculation of liquid mixing of different proportioning variable concentrations, every processing 3 strains, 3 leaves of often selecting good strains in the field for seed repeat 4 times, and 3-4 days " Invest, Then Investigate " withered spot numbers calculate inhibiting rate, use EC
50Value is calculated co-toxicity coefficient, and measurement result sees Table 12.
Virulence regression equation and the co-toxicity coefficient of table 12 reagent agent to tobacco virus
By data in table 12 as seen, malicious fluorine phosphorus and diethyl aminoethyl hexanoate are composite in above ratio, and co-toxicity coefficient antagonism namely do not occur all greater than 80.And co-toxicity coefficient all greater than 120, shows as synergy so that ratio was composite in 39.95: 0.05,39.93: 0.07, and when wherein ratio was composite with 39.95: 0.05, co-toxicity coefficient is maximum, and namely synergy is the most obvious.
Toxicity Determination embodiment 4:
Indoor pot paddy rice susceptible variety when rice shoot grows to 1 leaf 1 heart～2 leaf 1 heart, is carried out inoculated identification.Every alms bowl cover is with translucent cover, and gauze sealing in top calculates by 5 small brown rice planthoppers of every seedling the worm amount that needs, access small brown rice planthopper in 2～3 age (be with malicious rate approximately 40%).Expelling parasite every day 2 times makes tested rice seedling evenly be subjected to poison.The medicament for preparing variable concentrations is evenly sprayed on for the examination seedling, and every processing 50 strains repeat for 4 times, and after medicine, classification in the 14 days investigation state of an illness, calculate preventive effect, uses EC
50Value is calculated co-toxicity coefficient, and measurement result sees Table 13.
Virulence regression equation and the co-toxicity coefficient of table 13 reagent agent to stripe disease
By data in table 13 as seen, malicious fluorine phosphorus and Nafusaku are composite in above ratio, and co-toxicity coefficient antagonism namely do not occur all greater than 80.And co-toxicity coefficient is all greater than 120 so that ratio was composite in 29.98: 0.02,29.97: 0.03,29.96: 0.04, illustrates that this ratio is composite stripe disease is all had synergistic effect.
Toxicity Determination embodiment 5:
Indoor pot tobacco susceptible variety plant is to the 4-5 leaf phase, and with the isopyknic viral juice frictional inoculation of liquid mixing of different proportioning variable concentrations, every processing 3 strains, 3 leaves of often selecting good strains in the field for seed repeat 4 times, and 3-4 days " Invest, Then Investigate " withered spot numbers calculate inhibiting rate, use EC
50Value is calculated co-toxicity coefficient, and measurement result sees Table 14.
Virulence regression equation and the co-toxicity coefficient of table 14 reagent agent to tobacco virus
By data in table 14 as seen, malicious fluorine phosphorus and heteroauxin are composite in above ratio, and co-toxicity coefficient antagonism namely do not occur all greater than 80.Co-toxicity coefficient was greater than 120 when wherein ratio was composite with 69.995: 0.005, illustrated that this ratio is composite tobacco mosaic is had synergistic effect.
Toxicity Determination embodiment 6:
Indoor pot paddy rice susceptible variety when rice shoot grows to 1 leaf 1 heart～2 leaf 1 heart, is carried out inoculated identification.Every alms bowl cover is with translucent cover, and gauze sealing in top calculates by 5 white-backed planthoppers of every seedling the worm amount that needs, access white-backed planthopper in 2～3 age (be with malicious rate approximately 60%).Expelling parasite every day 2 times makes tested rice seedling evenly be subjected to poison.The medicament for preparing variable concentrations is evenly sprayed on for the examination seedling, and every processing 50 strains repeat for 4 times, and after medicine, classification in the 14 days investigation state of an illness, calculate preventive effect, uses EC
50Value is calculated co-toxicity coefficient, and measurement result sees Table 15.
Virulence regression equation and the co-toxicity coefficient of table 15 reagent agent to black streaked dwarf virus of rice
By data in table 15 as seen, malicious fluorine phosphorus and brassin lactones are composite in above ratio, and co-toxicity coefficient antagonism namely do not occur all greater than 80.And co-toxicity coefficient is all greater than 120 so that ratio was composite in 59.9997: 0.0003,59.9995: 0.0005,59.9993: 0.0007, illustrates that this ratio is composite black streaked dwarf virus of rice is all had synergistic effect.
Toxicity Determination embodiment 7:
Indoor pot paddy rice susceptible variety when rice shoot grows to 1 leaf 1 heart～2 leaf 1 heart, is carried out inoculated identification.Every alms bowl cover is with translucent cover, and gauze sealing in top calculates by 5 small brown rice planthoppers of every seedling the worm amount that needs, access small brown rice planthopper in 2～3 age (be with malicious rate approximately 40%).Expelling parasite every day 2 times makes tested rice seedling evenly be subjected to poison.The medicament for preparing variable concentrations is evenly sprayed on for the examination seedling, and every processing 50 strains repeat for 4 times, and after medicine, classification in the 14 days investigation state of an illness, calculate preventive effect, uses EC
50Value is calculated co-toxicity coefficient, and measurement result sees Table 16.
Virulence regression equation and the co-toxicity coefficient of table 16 reagent agent to stripe disease
By data in table 16 as seen, malicious fluorine phosphorus and gibberellin are composite in above ratio, and co-toxicity coefficient antagonism namely do not occur all greater than 80.And co-toxicity coefficient is all greater than 120 so that ratio was composite in 59.999: 0.006,59.999: 0.012,59.999: 0.024, illustrates that this ratio is composite stripe disease is all had synergistic effect.
Toxicity Determination embodiment 8:
Indoor pot tobacco susceptible variety plant is to the 4-5 leaf phase, and with the isopyknic viral juice frictional inoculation of liquid mixing of different proportioning variable concentrations, every processing 3 strains, 3 leaves of often selecting good strains in the field for seed repeat 4 times, and 3-4 days " Invest, Then Investigate " withered spot numbers calculate inhibiting rate, use EC
50Value is calculated co-toxicity coefficient, and measurement result sees Table 17.
Virulence regression equation and the co-toxicity coefficient of table 17 reagent agent to tobacco virus
By data in table 17 as seen, malicious fluorine phosphorus and triacontanol are composite in above ratio, and co-toxicity coefficient antagonism namely do not occur all greater than 80.Co-toxicity coefficient was greater than 120 when wherein ratio was composite with 34.993: 0.007, illustrated that this ratio is composite tobacco mosaic is had synergistic effect.
Toxicity Determination embodiment 9:
Indoor pot tobacco susceptible variety plant is to the 4-5 leaf phase, and with the isopyknic viral juice frictional inoculation of liquid mixing of different proportioning variable concentrations, every processing 3 strains, 3 leaves of often selecting good strains in the field for seed repeat 4 times, and 3-4 days " Invest, Then Investigate " withered spot numbers calculate inhibiting rate, use EC
50Value is calculated co-toxicity coefficient, and measurement result sees Table 18.
Virulence regression equation and the co-toxicity coefficient of table 18 reagent agent to tobacco virus
By data in table 18 as seen, malicious fluorine phosphorus and CPPU are composite in above ratio, and co-toxicity coefficient antagonism namely do not occur all greater than 80.Wherein co-toxicity coefficient greater than 120, illustrates that this ratio is composite tobacco mosaic is had synergistic effect so that ratio was composite in 29.92: 0.08,29.89: 0.11.
Toxicity Determination embodiment 10:
Indoor pot paddy rice susceptible variety when rice shoot grows to 1 leaf 1 heart～2 leaf 1 heart, is carried out inoculated identification.Every alms bowl cover is with translucent cover, and gauze sealing in top calculates by 5 white-backed planthoppers of every seedling the worm amount that needs, access white-backed planthopper in 2～3 age (be with malicious rate approximately 60%).Expelling parasite every day 2 times makes tested rice seedling evenly be subjected to poison.The medicament for preparing variable concentrations is evenly sprayed on for the examination seedling, and every processing 50 strains repeat for 4 times, and after medicine, classification in the 14 days investigation state of an illness, calculate preventive effect, uses EC
50Value is calculated co-toxicity coefficient, and measurement result sees Table 19.
Virulence regression equation and the co-toxicity coefficient of table 19 reagent agent to black streaked dwarf virus of rice
By data in table 19 as seen, malicious fluorine phosphorus and root-inducing powder are composite in above ratio, and co-toxicity coefficient antagonism namely do not occur all greater than 80.And co-toxicity coefficient is all greater than 120 so that ratio was composite in 54.97: 0.03,54.95: 0.05,54.93: 0.07, illustrates that this ratio is composite black streaked dwarf virus of rice is all had synergistic effect.
Toxicity Determination embodiment 11:
Indoor pot tobacco susceptible variety plant is to the 4-5 leaf phase, and with the isopyknic viral juice frictional inoculation of liquid mixing of different proportioning variable concentrations, every processing 3 strains, 3 leaves of often selecting good strains in the field for seed repeat 4 times, and 3-4 days " Invest, Then Investigate " withered spot numbers calculate inhibiting rate, use EC
50Value is calculated co-toxicity coefficient, and measurement result sees Table 20.
Virulence regression equation and the co-toxicity coefficient of table 20 reagent agent to tobacco virus
By data in table 20 as seen, malicious fluorine phosphorus and dragon flying around No. one are composite in above ratio, and co-toxicity coefficient antagonism namely do not occur all greater than 80.Wherein co-toxicity coefficient greater than 120, illustrates that this ratio is composite tobacco mosaic is had synergistic effect so that ratio was composite in 19.97: 0.03,19.96: 0.04.
Claims (5)
1. composition pesticide comprises: malicious fluorine phosphorus and growth regulator, and wherein malicious fluorine phosphorus accounts for the 20%-70% of composition by weight, and growth regulator accounts for the 0.0005%-2% of composition, wherein said growth regulator is selected from one or more in following material: brassin lactones, compound sodium nitrophenolate, diethyl aminoethyl hexanoate, the basic element of cell division, heteroauxin, Nafusaku, gibberellin, triacontanol, root-inducing powder, dragon flying around No. one and CPPU, the ratio of poison fluorine phosphorus and the basic element of cell division is 39.96: 0.04, the ratio of poison fluorine phosphorus and compound sodium nitrophenolate is 29.7: 0.3～29.3: 0.7, the ratio of poison fluorine phosphorus and diethyl aminoethyl hexanoate is 39.95: 0.05～39.93: 0.07, the ratio of poison fluorine phosphorus and Nafusaku is 29.98: 0.02～29.96: 0.04, the ratio of poison fluorine phosphorus and heteroauxin is 69.995: 0.005, the ratio of poison fluorine phosphorus and brassin lactones is 59.9997: 0.0003～59.9993: 0.0007, the ratio of poison fluorine phosphorus and gibberellin is 59.999: 0.006～59.999: 0.024, the ratio of poison fluorine phosphorus and triacontanol is 34.993: 0.007, the ratio of poison fluorine phosphorus and CPPU is 29.92: 0.08～29.89: 0.11, the ratio of poison fluorine phosphorus and root-inducing powder is 54.97: 0.03～54.93: 0.07, the ratio that poison fluorine phosphorus and dragon flying around are No. one is 19.97: 0.03～19.96: 0.04.
2. composition according to claim 1, is characterized in that described composition also contains one or more in acceptable surfactant, solvent and carrier on agricultural chemicals.
3. composition according to claim 2, it is characterized in that, described surfactant is one or more in dispersing agent NNO, lauryl sodium sulfate, dispersing agent MF, sodium lignin sulfonate, agriculture breast 600#, fatty alcohol-polyoxyethylene ether, naphthalene sulfonic acid formaldehyde condensation polymer, emulsifier 603#, calcium dodecyl benzene sulfonate 500#; Described solvent is one or more in isopropyl alcohol, dimethyl formamide, cyclohexanone, ethyl acetate, dimethylbenzene; Described carrier is one or more in white carbon, kaolin, water, potter's clay.
4. arbitrary described composition according to claim 1-3, is characterized in that, described composition is wetting powder, suspending agent, microemulsion, aqueous emulsion, water dispersible granules, dried suspension emulsion or plants the clothing agent.
5. the purposes aspect arbitrary described composition pesticide according to claim 1-3, the virus disease that is caused by oryza virus 3, fractilinea oryzae, MRDV poison, rice black-streaked dwarf virus, tomato virus disease poison, cucumber mosaic virus, tobacco mosaic virus in control.
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| CN102657219B (en) * | 2012-04-12 | 2013-11-27 | 广西田园生化股份有限公司 | Dufulin-containing ultra low volume liquid formulation |
| CN102986723B (en) * | 2012-11-08 | 2014-04-09 | 高润宝 | Application process of rice plant growth regulator |
| CN103125490A (en) * | 2013-03-15 | 2013-06-05 | 刘克 | Plant growth regulating agent and production process thereof |
| CN103814943B (en) * | 2014-02-13 | 2016-05-04 | 山东省农业科学院植物保护研究所 | A kind of composition pesticide and application thereof that prevents and treats the viroses of plant |
| CN103960296A (en) * | 2014-04-24 | 2014-08-06 | 山东圣鹏科技股份有限公司 | Plant virus disease resisting composition and application thereof |
| CN104285952B (en) * | 2014-09-15 | 2015-11-11 | 山东圣鹏科技股份有限公司 | A kind of anti-plant virus composition and application thereof |
| CN104521958A (en) * | 2014-12-09 | 2015-04-22 | 安徽扬子化工有限公司 | Application of guaiacol to tobacco mosaic virus |
| CN104756718B (en) * | 2015-04-13 | 2017-03-29 | 中国农业科学院蔬菜花卉研究所 | A kind of method of tomato cultivation |
| CN105028468B (en) * | 2015-06-17 | 2017-03-29 | 广西田园生化股份有限公司 | A kind of composition pesticide and bactericide containing toxic fluoride phosphate and dithiocarbamate bactericide |
| CN105052997B (en) * | 2015-07-31 | 2017-06-06 | 广西田园生化股份有限公司 | A kind of composition pesticide and bactericide containing toxic fluoride phosphate and antimicrobial fungicides |
| CN107517834A (en) * | 2015-08-28 | 2017-12-29 | 寿光市新世纪种苗有限公司 | A kind of breeding method of tomato health seedling |
| CN106035335B (en) * | 2016-06-02 | 2018-05-11 | 郑州卓越农业科技有限公司 | A kind of Compositional type plant growth promoter |
| CN111165491A (en) * | 2020-01-06 | 2020-05-19 | 海南正业中农高科股份有限公司 | Pesticide composition containing Vanilthioketal and plant growth regulator |
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