CN104823661A - Method for increasing drought-resistance capacity of plant - Google Patents
Method for increasing drought-resistance capacity of plant Download PDFInfo
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- CN104823661A CN104823661A CN201510223357.0A CN201510223357A CN104823661A CN 104823661 A CN104823661 A CN 104823661A CN 201510223357 A CN201510223357 A CN 201510223357A CN 104823661 A CN104823661 A CN 104823661A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
The invention discloses a method for increasing the drought-resistance capacity of a plant. The method comprises the following steps of carrying out inoculation and propagation expanding on a propagation expanding medium by utilizing red clover after respectively carrying out propagation expanding on white rot fungi, germs, glomus monosporum, glomus mosseae and acaulospora laevis and mixing the white rot fungi, the germs, the glomus monosporum, the glomus mosseae and the acaulospora laevis according to a ratio being 0.5:0.5:2:1:1, calculating the inoculum size to be 4%-6% of the propagation expanding medium in weight ratio, and finally obtaining a mycorrhiza fungi inoculant; adding 17g-21g of the mycorrhiza fungi inoculant which is already subjected to the propagation expanding in a culture medium according to the weight ratio being 4:1, seeding two plant seeds in each hole, and carrying out transplanting cultivation after the plant seeds are transplanted into a rooting culture medium for being continuously cultivated when the mycorrhizal infection rate of root systems is greater than 30%. According to the method disclosed by the invention, by adopting an AM fungal inoculum in host dependence, the drought resistance of the plant is increased, and the drought-resistance capacity of the plant is further increased in aspects of seed treatment, late cultivation and the like.
Description
Technical field
The present invention relates to field of plant cultivation, be specifically related to a kind of method improving drought tolerance in plants ability.
Background technology
Arbuscular mycorrhiza (Arbuscularmycorrhiza, AM) is a kind of association of the root growth of mycorrhizal fungi in soil and higher plant, is extensively be present in a kind of general plant symbiosis phenomenon of occurring in nature.Large quantity research finds, mycorhiza can Promoting plant growth, the resistance of enhancing plant, the raising moisture of plant and the ability such as the absorbing capacity of nutrient and improvement soil.At present, the restoration and reconstruction of degraded ecosystem enjoy concern both domestic and external, become one of modern ecology area research focus.AM inoculation technique is mycorrhizal seedling raising afforestation, opens an important channel for effectively solving Vegetation Restoration of Degraded Ecosystem.Utilize Mycorrhizal Technology to improve drought resisting and the drought resistance research that raw reproducting tree species are fitted in Mountainous Area, still rarely seen report.
Summary of the invention
For solving the problem, the invention provides a kind of method improving drought tolerance in plants ability, adopting the AM fungi Inoculant of Host Dependence to improve the drought resistance of plant, and further increasing the drought-resistant ability of plant from aspects such as seed treatment, later stage cultivations.
For achieving the above object, the technical scheme that the present invention takes is:
Improve a method for drought tolerance in plants ability, comprise the steps:
The preparation of S1, mycorrhizal fungi Inoculant:
Whiterot fungi, Glomus mosseae, light wall Acaulospora mould to germ, monospore sacculus are expanded respectively numerous after, after the ratio mixing of 0.5: 0.5: 2: 1: 1, red clover inoculation in the numerous matrix of expansion is utilized to expand numerous, inoculum concentration is calculated as with weight ratio and expands 4% ~ 6% of numerous matrix, final acquisition mycorrhizal fungi Inoculant, it to contain inside and outside 60 ~ 90 spores/10g dry ground and root mycelia and infects root segment; After expanding that numerous matrix effluent is husky and mixing in the ratio of 2L: 1L with loessal soil, add urea 53.8 ~ 81.7mg/kg, potassium dihydrogen phosphate 5.15 ~ 12.35mg/kg, boron zinc potassium calcium 73.4 ~ 123.5mg/kg, after organic 23.7 ~ 37.8g/kg, carry out hot air sterilization 4h under being placed in the condition of 160 ~ 180 DEG C and prepare gained;
S2, seed treatment:
Select plant seed, after mould impurity elimination, adopt carbendazim spraying to kill bulbil and the mycelia of attachment the surface of the seed, after clear water washing, get appropriate nutrient for plants and carry out immersion treatment 12h; Described nutrient for plants comprises 0.1 part of MnSO
4powder, 0.05 part of CuSO
4powder, 0.1 part of ZnSO
4powder, 0.2 part of NiSO
4powder, 0.2 part of (NH
4)
6mo
7o
2.4H
2o powder, appropriate EDTA and 300 ~ 400mL ionized water;
The cultivation of S3, mycorrhizal fungi microbial inoculum Mycorrhizal plantling:
In MS+6-BA0.4mgL-(-1)+NAA0.04mgL-(-1) medium by weight 4: 1 ratio add expand numerous after mycorrhizal fungi Inoculant 17g ~ 21g, each hole sowing plant seed 2, when root system Mycorrhizal Infection Incidence is greater than 30%, is moved in root media 1/2MS+IBA0.05mgL-(-1)+NAA0.02mgL-(-1) and continue to cultivate;
S4, seedling replanting:
Loosen the soil, the mode of the row such as the seedling of step S3 gained employing transplanted, 1 pipe 4 row or 5 row, two interlines are for laying irrigate band wholely; The dripping end flow of dropper band is selected according to the soil texture; After transplanting completes, fill with drought resisting nutritional agents with water droplet, described drought resisting nutritional agents is made up of the component of following weight percent concentration: salicylic acid 12.21% ~ 83.6%, acrylic acid 8.3% ~ 22.3%, humic acid 8.3% ~ 21.7%, Quercetin 7.0% ~ 12.0%, cyanidenon 7.0% ~ 12.0%, silicon potash fertilizer 46.2% ~ 184.8%, potassium dihydrogen phosphate 0.62% ~ 1.38%, urea 1% ~ 1.5%, zinc sulphate 0.25% ~ 0.55%, excess water.
The present invention has following beneficial effect:
The present invention adopts the AM fungi Inoculant of Host Dependence to improve the drought resistance of plant, by the use to germ and whiterot fungi, improve the absorption rate of crop to nutritive element, by the seed soaking of seed-soaking liquid, thus facilitate the sprouting of seed, improve respiratory rate and the root activity of seedling, enhance the anti-adversity ability of seed and seedling, by a tank of drought resisting nutritional agents, phenolic acid class is utilized to have antioxidants, quantity and the activity of protective enzyme in plant can be improved, turn improve the content of polyphenol substance, improve Antioxidants in crop, enhance the drought resistance of crop, meanwhile, in plant, the raising of phenols Antioxidants can increase crop disease-resistant, uv-resistant radiation etc., in phenolic acid, with the addition of potassium dihydrogen phosphate, nitrogen, add the nutritive element supply of crop, facilitate the production of crop, improve the resistance of crop.
Embodiment
In order to make objects and advantages of the present invention clearly understand, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Sunflower diseased plant is come to germ sampling in the embodiment of the present invention; Whiterot fungi sampling comes from rotten trees;
To germ: find in sunflower stem, this bacterium can make sunflower bend, and the lignocellulose in degraded sunflower stem, therefore called after is to germ.This bacterial classification also plants for our research team the bacterial classification independently separated, and have remarkable degradation effect to wood fibre, and in the art of this patent, effect is obvious, simultaneously also as the pilot scale of this bacterial screening experimental study.
Whiterot fungi: screen in the trees of rotting and obtain, this bacterium can make the trees of rotting become white, therefore called after whiterot fungi.
After testing, be 30.2% to germ to the degradation rate of lignin in reed, whiterot fungi is 26.7% to the degradation rate of lignin in reed.
Embodiment 1
The preparation of S1, mycorrhizal fungi Inoculant:
Whiterot fungi, Glomus mosseae, light wall Acaulospora mould to germ, monospore sacculus are expanded respectively numerous after, after the ratio mixing of 0.5: 0.5: 2: 1: 1, red clover inoculation in the numerous matrix of expansion is utilized to expand numerous, inoculum concentration is calculated as with weight ratio and expands 4% of numerous matrix, final acquisition mycorrhizal fungi Inoculant, it to contain inside and outside 60 spores/10g dry ground and root mycelia and infects root segment; After expanding that numerous matrix effluent is husky and mixing in the ratio of 2L: 1L with loessal soil, add urea 53.8mg/kg, potassium dihydrogen phosphate 5.15mg/kg, boron zinc potassium calcium 73.4mg/kg, after organic 23.7g/kg, carries out hot air sterilization 4h under being placed in the condition of 160 DEG C and prepares gained;
S2, seed treatment:
Select plant seed, after mould impurity elimination, adopt carbendazim spraying to kill bulbil and the mycelia of attachment the surface of the seed, after clear water washing, get appropriate nutrient for plants and carry out immersion treatment 12h; Described nutrient for plants comprises 0.1 part of MnSO
4powder, 0.05 part of CuSO
4powder, 0.1 part of ZnSO
4powder, 0.2 part of NiSO
4powder, 0.2 part of (NH
4)
6mo
7o
2.4H
2o powder, appropriate EDTA and 300 ~ 400mL ionized water;
The cultivation of S3, mycorrhizal fungi microbial inoculum Mycorrhizal plantling:
In MS+6-BA0.4mgL-(-1)+NAA0.04mgL-(-1) medium by weight 4: 1 ratio add expand numerous after mycorrhizal fungi Inoculant 17g ~ 21g, each hole sowing plant seed 2, when root system Mycorrhizal Infection Incidence is greater than 30%, is moved in root media 1/2MS+IBA0.05mgL-(-1)+NAA0.02mgL-(-1) and continue to cultivate;
S4, seedling replanting:
Loosen the soil, the mode of the row such as the seedling of step S3 gained employing transplanted, 1 pipe 4 row or 5 row, two interlines are for laying irrigate band wholely; The dripping end flow of dropper band is selected according to the soil texture; After transplanting completes, fill with drought resisting nutritional agents with water droplet, described drought resisting nutritional agents is made up of the component of following weight percent concentration: salicylic acid 12.21%, acrylic acid 8.3%, humic acid 8.3%, Quercetin 7.0%, cyanidenon 7.0%, silicon potash fertilizer 46.2%, potassium dihydrogen phosphate 0.62%, urea 1%, zinc sulphate 0.25%, excess water.
Embodiment 2
The preparation of S1, mycorrhizal fungi Inoculant:
Whiterot fungi, Glomus mosseae, light wall Acaulospora mould to germ, monospore sacculus are expanded respectively numerous after, after the ratio mixing of 0.5: 0.5: 2: 1: 1, red clover inoculation in the numerous matrix of expansion is utilized to expand numerous, inoculum concentration is calculated as with weight ratio and expands 6% of numerous matrix, final acquisition mycorrhizal fungi Inoculant, it to contain inside and outside 90 spores/10g dry ground and root mycelia and infects root segment; After expanding that numerous matrix effluent is husky and mixing in the ratio of 2L: 1L with loessal soil, add urea 81.7mg/kg, potassium dihydrogen phosphate 12.35mg/kg, boron zinc potassium calcium 123.5mg/kg, after organic 37.8g/kg, carries out hot air sterilization 4h under being placed in the condition of 180 DEG C and prepares gained;
S2, seed treatment:
Select plant seed, after mould impurity elimination, adopt carbendazim spraying to kill bulbil and the mycelia of attachment the surface of the seed, after clear water washing, get appropriate nutrient for plants and carry out immersion treatment 12h; Described nutrient for plants comprises 0.1 part of MnSO
4powder, 0.05 part of CuSO
4powder, 0.1 part of ZnSO
4powder, 0.2 part of NiSO
4powder, 0.2 part of (NH
4)
6mo
7o
2.4H
2o powder, appropriate EDTA and 400mL ionized water;
The cultivation of S3, mycorrhizal fungi microbial inoculum Mycorrhizal plantling:
In MS+6-BA0.4mgL-(-1)+NAA0.04mgL-(-1) medium by weight 4: 1 ratio add expand numerous after mycorrhizal fungi Inoculant 21g, each hole sowing plant seed 2, when root system Mycorrhizal Infection Incidence is greater than 30%, is moved in root media 1/2MS+IBA0.05mgL-(-1)+NAA0.02mgL-(-1) and continue to cultivate;
S4, seedling replanting:
Loosen the soil, the mode of the row such as the seedling of step S3 gained employing transplanted, 1 pipe 4 row or 5 row, two interlines are for laying irrigate band wholely; The dripping end flow of dropper band is selected according to the soil texture; After transplanting completes, fill with drought resisting nutritional agents with water droplet, described drought resisting nutritional agents is made up of the component of following weight percent concentration: salicylic acid 83.6%, acrylic acid 22.3%, humic acid 21.7%, Quercetin 12.0%, cyanidenon 12.0%, silicon potash fertilizer 184.8%, potassium dihydrogen phosphate 1.38%, urea 1.5%, zinc sulphate 0.55%, excess water.
Embodiment 3
The preparation of S1, mycorrhizal fungi Inoculant:
Whiterot fungi, Glomus mosseae, light wall Acaulospora mould to germ, monospore sacculus are expanded respectively numerous after, after the ratio mixing of 0.5: 0.5: 2: 1: 1, red clover inoculation in the numerous matrix of expansion is utilized to expand numerous, inoculum concentration is calculated as with weight ratio and expands 5% of numerous matrix, final acquisition mycorrhizal fungi Inoculant, it to contain inside and outside 75 spores/10g dry ground and root mycelia and infects root segment; After expanding that numerous matrix effluent is husky and mixing in the ratio of 2L: 1L with loessal soil, add urea 67.75mg/kg, potassium dihydrogen phosphate 8.75mg/kg, boron zinc potassium calcium 98.45mg/kg, after organic 30.75g/kg, carry out hot air sterilization 4h under being placed in the condition of 170 DEG C and prepare gained;
S2, seed treatment:
Select plant seed, after mould impurity elimination, adopt carbendazim spraying to kill bulbil and the mycelia of attachment the surface of the seed, after clear water washing, get appropriate nutrient for plants and carry out immersion treatment 12h; Described nutrient for plants comprises 0.1 part of MnSO
4powder, 0.05 part of CuSO
4powder, 0.1 part of ZnSO
4powder, 0.2 part of NiSO
4powder, 0.2 part of (NH
4)
6mo
7o
2.4H
2o powder, appropriate EDTA and 300 ~ 400mL ionized water;
The cultivation of S3, mycorrhizal fungi microbial inoculum Mycorrhizal plantling:
In MS+6-BA0.4mgL-(-1)+NAA0.04mgL-(-1) medium by weight 4: 1 ratio add expand numerous after mycorrhizal fungi Inoculant 17g ~ 21g, each hole sowing plant seed 2, when root system Mycorrhizal Infection Incidence is greater than 30%, is moved in root media 1/2MS+IBA0.05mgL-(-1)+NAA0.02mgL-(-1) and continue to cultivate;
S4, seedling replanting:
Loosen the soil, the mode of the row such as the seedling of step S3 gained employing transplanted, 1 pipe 4 row or 5 row, two interlines are for laying irrigate band wholely; The dripping end flow of dropper band is selected according to the soil texture; After transplanting completes, fill with drought resisting nutritional agents with water droplet, described drought resisting nutritional agents is made up of the component of following weight percent concentration: salicylic acid 47.905%, acrylic acid 15.3%, humic acid 15%, Quercetin 9.5%, cyanidenon 9.5%, silicon potash fertilizer 115.5%, potassium dihydrogen phosphate 1%, urea 1.45%, zinc sulphate 0.8%, excess water.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (1)
1. improve a method for drought tolerance in plants ability, it is characterized in that, comprise the steps:
The preparation of S1, mycorrhizal fungi Inoculant:
Whiterot fungi, Glomus mosseae, light wall Acaulospora mould to germ, monospore sacculus are expanded respectively numerous after, after the ratio mixing of 0.5: 0.5: 2: 1: 1, red clover inoculation in the numerous matrix of expansion is utilized to expand numerous, inoculum concentration is calculated as with weight ratio and expands 4% ~ 6% of numerous matrix, final acquisition mycorrhizal fungi Inoculant, it to contain inside and outside 60 ~ 90 spores/10g dry ground and root mycelia and infects root segment; After expanding that numerous matrix effluent is husky and mixing in the ratio of 2L: 1L with loessal soil, add urea 53.8 ~ 81.7mg/kg, potassium dihydrogen phosphate 5.15 ~ 12.35mg/kg, boron zinc potassium calcium 73.4 ~ 123.5mg/kg, after organic 23.7 ~ 37.8g/kg, carry out hot air sterilization 4h under being placed in the condition of 160 ~ 180 DEG C and prepare gained;
S2, seed treatment:
Select plant seed, after mould impurity elimination, adopt carbendazim spraying to kill bulbil and the mycelia of attachment the surface of the seed, after clear water washing, get appropriate nutrient for plants and carry out immersion treatment 12h; Described nutrient for plants comprises 0.1 part of MnSO
4powder, 0.05 part of CuSO
4powder, 0.1 part of ZnSO
4powder, 0.2 part of NiSO
4powder, 0.2 part of (NH
4)
6mo
7o
2.4H
2o powder, appropriate EDTA and 300 ~ 400mL ionized water;
The cultivation of S3, mycorrhizal fungi microbial inoculum Mycorrhizal plantling:
In MS+6-BA0.4mgL-(-1)+NAA0.04mgL-(-1) medium by weight 4: 1 ratio add expand numerous after mycorrhizal fungi Inoculant 17g ~ 21g, each hole sowing plant seed 2, when root system Mycorrhizal Infection Incidence is greater than 30%, is moved in root media 1/2MS+IBA0.05mgL-(-1)+NAA0.02mgL-(-1) and continue to cultivate;
S4, seedling replanting:
Loosen the soil, the mode of the row such as the seedling of step S3 gained employing transplanted, 1 pipe 4 row or 5 row, two interlines are for laying irrigate band wholely; The dripping end flow of dropper band is selected according to the soil texture; After transplanting completes, fill with drought resisting nutritional agents with water droplet, described drought resisting nutritional agents is made up of the component of following weight percent concentration: salicylic acid 12.21% ~ 83.6%, acrylic acid 8.3% ~ 22.3%, humic acid 8.3% ~ 21.7%, Quercetin 7.0% ~ 12.0%, cyanidenon 7.0% ~ 12.0%, silicon potash fertilizer 46.2% ~ 184.8%, potassium dihydrogen phosphate 0.62% ~ 1.38%, urea 1% ~ 1.5%, zinc sulphate 0.25% ~ 0.55%, excess water.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105123222A (en) * | 2015-08-27 | 2015-12-09 | 苏州高创特新能源发展股份有限公司 | Application of switchgrass in desert control |
| CN105993749A (en) * | 2016-05-10 | 2016-10-12 | 广西兆和种业有限公司 | Paddy rice cultivation method |
| CN106576757A (en) * | 2016-11-24 | 2017-04-26 | 刘雄 | Bletilla striata planting method |
| CN109275408A (en) * | 2018-11-21 | 2019-01-29 | 南京农业大学 | A kind of extremely simplified fertilizing method of beach salination middle-and-low-yielding fields wheat |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103011925A (en) * | 2013-01-15 | 2013-04-03 | 内蒙古自治区林业科学研究院 | Preparation method of biomass sandy soil improving agent |
| CN103828618A (en) * | 2014-03-26 | 2014-06-04 | 兰州大学 | Method for improving growth and drought-resistant ability of wheat by using arbuscular mycorrhizal fungi |
| CN103865812A (en) * | 2014-04-02 | 2014-06-18 | 兰州大学 | Greenhouse propagation method for arbuscular mycorrhizal fungus glomus versiforme microbial inoculum |
| CN104003799A (en) * | 2014-05-27 | 2014-08-27 | 河南农业大学 | Nutritive drought resistant agent for crops |
| CN104488525A (en) * | 2014-12-13 | 2015-04-08 | 山西省农业科学院高寒区作物研究所 | Culture method of confectionary sunflower |
-
2015
- 2015-04-29 CN CN201510223357.0A patent/CN104823661A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103011925A (en) * | 2013-01-15 | 2013-04-03 | 内蒙古自治区林业科学研究院 | Preparation method of biomass sandy soil improving agent |
| CN103828618A (en) * | 2014-03-26 | 2014-06-04 | 兰州大学 | Method for improving growth and drought-resistant ability of wheat by using arbuscular mycorrhizal fungi |
| CN103865812A (en) * | 2014-04-02 | 2014-06-18 | 兰州大学 | Greenhouse propagation method for arbuscular mycorrhizal fungus glomus versiforme microbial inoculum |
| CN104003799A (en) * | 2014-05-27 | 2014-08-27 | 河南农业大学 | Nutritive drought resistant agent for crops |
| CN104488525A (en) * | 2014-12-13 | 2015-04-08 | 山西省农业科学院高寒区作物研究所 | Culture method of confectionary sunflower |
Non-Patent Citations (5)
| Title |
|---|
| 丁春邦等: "《植物学》", 28 February 2015, 中国农业出版社 * |
| 张延旭等: "接种丛枝菌根对玉米生长与抗旱性的影响", 《干旱地区农业研究》 * |
| 白安龙: "东北大庆膜下滴灌玉米栽培技术初探", 《现代农业》 * |
| 苏艳红等: "旱地玉米抗旱措施研究进展", 《江西农业学报》 * |
| 黄艳胜: "不同浓度营养液对玉米种子萌发及幼苗生长影响的研究", 《黑龙江农业科学》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105123222A (en) * | 2015-08-27 | 2015-12-09 | 苏州高创特新能源发展股份有限公司 | Application of switchgrass in desert control |
| CN105993749A (en) * | 2016-05-10 | 2016-10-12 | 广西兆和种业有限公司 | Paddy rice cultivation method |
| CN106576757A (en) * | 2016-11-24 | 2017-04-26 | 刘雄 | Bletilla striata planting method |
| CN109275408A (en) * | 2018-11-21 | 2019-01-29 | 南京农业大学 | A kind of extremely simplified fertilizing method of beach salination middle-and-low-yielding fields wheat |
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