CN101623003B - Method and special seed soaking liquid for promoting germination of cucumber seeds under the condition of salt stress - Google Patents
Method and special seed soaking liquid for promoting germination of cucumber seeds under the condition of salt stress Download PDFInfo
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- CN101623003B CN101623003B CN200910091268XA CN200910091268A CN101623003B CN 101623003 B CN101623003 B CN 101623003B CN 200910091268X A CN200910091268X A CN 200910091268XA CN 200910091268 A CN200910091268 A CN 200910091268A CN 101623003 B CN101623003 B CN 101623003B
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Abstract
The invention discloses a method and a special seed soaking agent for promoting germination of cucumber seeds under the condition of salt stress. The seed soaking agent comprises calcium chloride, L-glutamic acid and water, wherein the molar concentration ratio of the calcium chloride to the L-glutamic acid is 0.5:5-20, preferably 0.5:10. The method for promoting germination of cucumber seeds under the condition of salt stress comprises the step that the seed soaking agent for promoting germination of cucumber seeds under the condition of salt stress is used to soak the cucumber seeds before seeding. The method for promoting germination of cucumber seeds under the condition of salt stress can obviously improve the germination ratio of the cucumber seeds under the condition of salt stress, and the germination ratio is more than 45%. The method is very favorable for improving the utilization ratio of resources and provides the guarantee for vegetable production in salinized areas.
Description
Technical field
The present invention relates to a kind of method and special seed soaking liquid that promotes the condition of salt stress germination of cucumber seeds under.
Background technology
Because environmental pollution, the salination of soil has become a worldwide problem, the area in all kinds of saline lands of China also constantly increase (Yang Shaohui etc. salt stress is to the progress [J] of plant influence. Molecular Plant Breeding, 2006,4:139-142).Also do not seek unity of standard about the concentration limit of salt stress at present, different plant varieties show different susceptibility to salt stress.It is generally acknowledged in mineral salt to plant hazard maximum be NaCl, even the low salinity of 50mM NaCl for the stronger paddy rice of salt resistance also be cause death (the woods wind etc. of dwelling. plant salt endurance progress [J]. the biotechnology progress, 2005,20:20-25).A large amount of researchs prove; Most greengrocery plant all shows susceptibility to salt; Cucumber as one of main vegetable variety to salt stress show very strong susceptibility (Dajic Zora.Salt stress.Physiology and Molecular Biology of Stress Tolerance in Plants [M] .Printed in the Netherlands.2006,41-99).Under condition of salt stress, moisture and ionic absorption are changed in the plant cell, cause high osmotic stress, ion to poison, thereby reduced seed germination rate.Therefore, improving salt marsh condition germination of cucumber seeds under rate, improve the salt resistance ability of crop itself, is one of cost-effective method in improvement salt stress soil.
Summary of the invention
The purpose of this invention is to provide a kind of method and special seed soaking agent thereof that promotes the condition of salt stress germination of cucumber seeds under.
The seed soaking agent of promotion condition of salt stress germination of cucumber seeds under provided by the invention comprises calcium chloride, L-glutamic acid and water.
This seed soaking agent can only be made up of above-mentioned three components.Wherein, the molar concentration rate of calcium chloride and L-glutamic acid is 0.5: 5-20, preferred 0.5: 10.
The method of promotion condition of salt stress germination of cucumber seeds under provided by the invention is prior to seeding, with the seed soaking agent of above-mentioned promotion condition of salt stress germination of cucumber seeds under provided by the invention cucumber seeds is soaked seed.
In this method, the time of seed soaking is 12-24 hour, and the temperature of seed soaking is 20-28 ℃.As when temperature is 28 ℃, optimum seed soaking time is 12 hours.
The method of promotion salt stress germination of cucumber seeds under provided by the invention can significantly improve the germination rate of cucumber seeds under the condition of salt stress, all more than 45%.This method is very beneficial for improving the availability of resource, and provides safeguard for the regional vegetables of salination produce.
Embodiment
Below in conjunction with specific embodiment the present invention is described further, but the present invention is not limited to following examples.
Embodiment 1,
Below in conjunction with specific embodiment the present invention is described further, but the present invention is not limited to following examples.Used putrescine is all available from sigma company among the present invention.
Embodiment 1
The cucumber variety that experiment is selected for use is middle peasant No. 8, from the Chinese Academy of Agricultural Sciences.Get No. 8 cucumber seeds of middle peasant of full unanimity, after the clorox sterilization, distilled water is cleaned, and above-mentioned cucumber seeds is soaked with following seed-soaking liquid a, b and c respectively, and seed soaking time is 24 hours, and soaking temperature is 20 ℃.
Seed-soaking liquid a, b and c are respectively:
A, water;
B, concentration are the CaCl of 0.5mM
2The aqueous solution;
C, L-glutamic acid and CaCl
2The aqueous solution; Wherein, the concentration of L-glutamic acid is 5mM, CaCl
2Concentration be 0.5mM.
Above-mentioned seed-soaking liquid a, b and c are provided with 4 repetitions respectively, and each all adds the 5ml seed-soaking liquid in repeating.
After seed soaking is accomplished; Diameter be 9 centimetres at culture dish, put into 2 metafiltration paper in the culture dish, after filter paper uses concentration to soak as the NaCl aqueous solution of 200mM; The cucumber seeds that evenly sets after above-mentioned seed-soaking liquid a, b and c soak seed carries out vernalization, checks the seed sprouting situation after 48 hours and calculates germination rate.It is standard that seed sprouting shows money or valuables one carries unintentionally with seed, and promptly the bud of cucumber seeds reaches two of seed length/for the moment, promptly be regarded as seed and sprout.
Through calculating, after above-mentioned seed-soaking liquid a, b and c seed soaking, the germination rate of seed is respectively 37.8%, 38.9% and 50%.Thereby seed soaking fluid power provided by the invention significantly improves the germination rate of cucumber seeds under the condition of salt stress.
Embodiment 2
The cucumber variety that experiment is selected for use is middle peasant No. 8, from the Chinese Academy of Agricultural Sciences.Get No. 8 cucumber seeds of middle peasant of full unanimity, after the clorox sterilization, distilled water is cleaned, and above-mentioned cucumber seeds is soaked with following seed-soaking liquid a, b and c respectively, and seed soaking time is 12 hours, and soaking temperature is 28 ℃.
Seed-soaking liquid a, b and c are respectively:
A, water;
B, concentration are the CaCl of 0.5mM
2The aqueous solution;
C, L-glutamic acid and CaCl
2The aqueous solution; Wherein, the concentration of L-glutamic acid is 10mM, CaCl
2Concentration be 0.5mM.
Above-mentioned seed-soaking liquid a, b and c are provided with 4 repetitions respectively, and each all adds the 5ml seed-soaking liquid in repeating.
After seed soaking is accomplished; Diameter be 9 centimetres at culture dish, put into 2 metafiltration paper in the culture dish, after filter paper uses concentration to soak as the NaCl aqueous solution of 200mM; The cucumber seeds that evenly sets after above-mentioned seed-soaking liquid a, b and c soak seed carries out vernalization, checks the seed sprouting situation after 48 hours and calculates germination rate.It is standard that seed sprouting shows money or valuables one carries unintentionally with seed, and promptly the bud of cucumber seeds reaches two of seed length/for the moment, promptly be regarded as seed and sprout.
Through calculating, after above-mentioned seed-soaking liquid a, b and c seed soaking, the germination rate of seed is respectively 39.5%, 40% and 60%.Thereby seed soaking fluid power provided by the invention significantly improves the germination rate of cucumber seeds under the salt stress.
Embodiment 3
The cucumber variety that experiment is selected for use is middle peasant No. 8, from the Chinese Academy of Agricultural Sciences.Get No. 8 cucumber seeds of middle peasant of full unanimity, after the clorox sterilization, distilled water is cleaned, and above-mentioned cucumber seeds is soaked with following seed-soaking liquid a, b and c respectively, and seed soaking time is 18 hours, and soaking temperature is 25 ℃.
Seed-soaking liquid a, b and c are respectively:
A, water;
B, concentration are the CaCl of 0.5mM
2The aqueous solution;
C, L-glutamic acid and CaCl
2The aqueous solution; Wherein, the concentration of L-glutamic acid is 20mM, CaCl
2Concentration be 0.5mM.
Above-mentioned seed-soaking liquid a, b and c are provided with 4 repetitions respectively, and each all adds the 5ml seed-soaking liquid in repeating.
After seed soaking is accomplished; Diameter be 9 centimetres at culture dish, put into 2 metafiltration paper in the culture dish, after filter paper uses concentration to soak as the NaCl aqueous solution of 200mM; The cucumber seeds that evenly sets after above-mentioned seed-soaking liquid a, b and c soak seed carries out vernalization, checks the seed sprouting situation after 48 hours and calculates germination rate.It is standard that seed sprouting shows money or valuables one carries unintentionally with seed, and promptly the bud of cucumber seeds reaches two of seed length/for the moment, promptly be regarded as seed and sprout.
Through calculating, after above-mentioned seed-soaking liquid a, b and c seed soaking, the germination rate of seed is respectively 40.6%, 41.2 and 45%.Thereby seed soaking fluid power provided by the invention significantly improves the germination rate of cucumber seeds under the salt stress.
Claims (5)
1. a seed soaking agent that promotes the condition of salt stress germination of cucumber seeds under is made up of calcium chloride, L-glutamic acid and water, and the molar concentration rate of said calcium chloride and said L-glutamic acid is 0.5: 5-20.
2. seed soaking agent according to claim 1 is characterized in that: in the said seed soaking agent, the molar concentration rate of calcium chloride and L-glutamic acid is 0.5: 10.
3. a method that promotes the condition of salt stress germination of cucumber seeds under is prior to seeding, with the seed soaking agent of the said promotion condition of salt stress of claim 1 germination of cucumber seeds under cucumber seeds is soaked seed.
4. method according to claim 3 is characterized in that: the time of said seed soaking is 12-24 hour.
5. according to claim 3 or 4 described methods, it is characterized in that: the temperature of said seed soaking is 20-28 ℃.
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| Application Number | Priority Date | Filing Date | Title |
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| CN200910091268XA CN101623003B (en) | 2009-08-14 | 2009-08-14 | Method and special seed soaking liquid for promoting germination of cucumber seeds under the condition of salt stress |
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| CN200910091268XA CN101623003B (en) | 2009-08-14 | 2009-08-14 | Method and special seed soaking liquid for promoting germination of cucumber seeds under the condition of salt stress |
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| CN101623003A CN101623003A (en) | 2010-01-13 |
| CN101623003B true CN101623003B (en) | 2012-07-11 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107535493A (en) * | 2017-09-30 | 2018-01-05 | 四川农业大学 | A kind of composition and its application in Trifolium repense seed under improving salt stress is sprouted |
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| CN103283784A (en) * | 2013-06-24 | 2013-09-11 | 合肥多加农业科技有限公司 | Long-acting environment-friendly paddy-rice seed-soaking agent and application method thereof |
| CN104521373B (en) * | 2015-02-03 | 2016-03-16 | 天津农学院 | The control method of constant salinity under the residing sand matrix of a kind of corn germination test |
| CN107087641B (en) * | 2017-02-08 | 2021-04-13 | 福建三炬生物科技股份有限公司 | Marine oligosaccharide biological preparation for improving salt resistance of crops and preparation method thereof |
| CN107041182A (en) * | 2017-05-18 | 2017-08-15 | 中国科学院东北地理与农业生态研究所 | A kind of method that nursery of soaking seed improves plant seedling stage salinity and alkalinity resistance |
| CN107409532A (en) * | 2017-07-21 | 2017-12-01 | 广西柳城县成霖农业科技有限公司 | A kind of seed-soaking method for improving Soybean Resistance Saline Alkali Stress ability |
| CN110383975B (en) * | 2018-04-18 | 2021-08-17 | 江苏省农业科学院 | Method for promoting lutein enrichment of corn kernels |
| CN111937884B (en) * | 2020-08-24 | 2022-01-07 | 江苏明德立达作物科技有限公司 | Plant growth regulating composition for cucumber, preparation method and application thereof |
| CN115886002A (en) * | 2022-08-18 | 2023-04-04 | 广东省农业科学院农业质量标准与监测技术研究所 | Application of seed soaking liquid in relieving arsenic stress of fragrant rice seedlings and improving rice fragrance |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101099491A (en) * | 2006-07-07 | 2008-01-09 | 东海生物科技股份有限公司 | Method for enhancing the growth of crops, plants, or seeds, and soil renovation |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101099491A (en) * | 2006-07-07 | 2008-01-09 | 东海生物科技股份有限公司 | Method for enhancing the growth of crops, plants, or seeds, and soil renovation |
Non-Patent Citations (4)
| Title |
|---|
| Dajic Zora..Physiology and Molecular Biology of Stress Tolerance in Plants.《Salt stress》.2006,41-99. * |
| 杨少辉 等.盐胁迫对植物影响的研究进展.《分子植物育种》.2006,第4卷(第3(s)期),139-142. * |
| 杨晓慧 等.植物对盐胁迫的反应及其抗盐机理研究进展.《山东农业大学学报（自然科学版）》.2006,第37卷(第2期),302-305. * |
| 项俊 等.水分和氯化钙胁迫对麦冬抗逆性生理指标的影响.《现代生物医学进展》.2009,第9卷(第1期),59-62. * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107535493A (en) * | 2017-09-30 | 2018-01-05 | 四川农业大学 | A kind of composition and its application in Trifolium repense seed under improving salt stress is sprouted |
| CN107535493B (en) * | 2017-09-30 | 2019-11-05 | 四川农业大学 | A kind of composition and its application in the case where improving salt stress in the sprouting of Trifolium repense seed |
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| CN101623003A (en) | 2010-01-13 |
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