CN109287192A - A kind of method of cotton seeds Identification of Drought - Google Patents
A kind of method of cotton seeds Identification of Drought Download PDFInfo
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- CN109287192A CN109287192A CN201811460624.6A CN201811460624A CN109287192A CN 109287192 A CN109287192 A CN 109287192A CN 201811460624 A CN201811460624 A CN 201811460624A CN 109287192 A CN109287192 A CN 109287192A
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- drought
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
- A01C1/025—Testing seeds for determining their viability or germination capacity
Abstract
The invention discloses a kind of method of cotton seeds Identification of Drought, belong to seed screening and assessment technique field in agriculture field.Method is as follows: from well-mixed net seed, random number takes the cotton seed of full seed, then seed is divided into polyethylene glycol stress processing and control treatment group, it sets and is cultivated in the light incubator, after cultivating the predetermined time, the chitting piece number of each processing is investigated, calculates drought resistance coefficient, it is compared by the analysis of drought resistance coefficient, characterization and evaluation is carried out to the drought resistance of cotton seeds.This method is at low cost, and the period is short, easily operated, convenient for being especially suitable for the screening of drought resistance of extensive germ plasm resource in each local promotion and implementation.
Description
Technical field
The present invention relates to a kind of methods more particularly to a kind of method of cotton seeds Identification of Drought for identifying seed, belong to
Seed screening and assessment technique field in agriculture field.
Background technique
Arid is a global problem.According to statistics, global arid and semi-arid arable land accounts for world's total area under cultivation
42.9%, China arid and semi-arid area area accounts for the 48% of national total area under cultivation, and drought stress is damaged caused by crops
Mistake occupies first place in all abiotic stress.Cotton is as most important natural fiber crop and most important economy in the world
Crop, it is both the raw material of the industrial departments such as primary raw material and national defence, medicine, the chemical industry of textile industry.
However, as China's cultivated area increasingly reduces, it is more and more prominent that grain and cotton strives ground contradiction, for the grain for ensuring China
Safety, cotton planting are gradually shifted to western nonirrigated farmland, and arid has become the essential environmental factors for influencing Cotton Production.
For a long time, domestic and foreign scholars have made extensive work in terms of Drought Resistance Germplasm screening and identification, horizontal from different perspectives
Propose many identification methods and index, have using morphological feature for example plant height, fruit joint number, single-strain bell-forming number, the flowering and boll-setting period number of blade,
Effective fruit branch number as Drought Resistant Identification, have referred to using yield such as seed, Single boll weight, harvest index are as Drought Resistant Identification,
Have using Physiology and biochemistry method such as proline, soluble sugar, malonaldehyde, catalase etc. as Drought Resistant Identification, but extremely
The present does not form a set of accurate, reliable, simple, generally acknowledged, unified identification method and index system.
Currently, screening or identification to drought resisting cotton material, are to carry out the time of infertility using drought area field
Identification, or artificial non-irrigated canopy carry out Seedling Stage test.The cotton time of infertility and non-irrigated canopy identification meet production reality, and evaluation is more reliable,
But annual only one season of growth of cotton, the period is long, takes up a large area, and determination rates are low, at high cost, can only material compared with
It is used when few, and the variation in time and weather is affected, the quality and field capacity of soil are closely related, and moisture is not easy to unite
One, year between and parallel test between result comparativity it is poor.
Therefore there is an urgent need to develop it is a kind of it is at low cost, the period is short, cotton seeds easily operated, accuracy in detection is high it is anti-
Drought identification method.
Summary of the invention
The purpose of the present invention is to provide a kind of methods of cotton seeds Identification of Drought, it is intended to solve traditional identification skill
Art environment is not easy to control, and qualification cycle is long, low efficiency, the big disadvantage of human error.The culture environment of this method is easy unified control
System, qualification cycle is short and easily operated, testing result is more accurate, is convenient for promotion and implementation, is especially suitable for extensive germ plasm resource
Screening of drought resistance.
The present invention, the method for cotton seeds Identification of Drought, using various concentration polyglycol solution to cotton seeds into
Row stress and control culture, are compared using drought resistance coefficient, evaluate cotton seeds drought resistance.
A kind of method of cotton seeds Identification of Drought, which comprises the steps of:
(1) preparation of samples:
From well-mixed net seed, random number takes cotton seeds number；
(2) coercing cultivation:
Cotton seeds are planted into the casting bed made of certain density polyglycol solution, illumination box culture are placed in, to son
Leaf exposes the illumination of progress certain time behind sand face；
(3) control culture:
Cotton seed is planted into the casting bed culture made of clear water, is placed in illumination box culture, carries out one after cotyledon exposes sand face
The illumination fixed time；
(4) investigation statistics:
After cultivating the predetermined time, chitting piece number is investigated；
(5) result calculating and characterization and evaluation:
Calculating pats mean value everywhere, and calculates drought resistance coefficient GI as follows:
GI = GDS/GCK ×100
Wherein: GDSFor duplicate average germination percentage under drought stress processing, GCKFor average germination percentage duplicate under control treatment；
In step (1), the cotton seeds that take of number are the cotton seed of full seed, and quality meets GB4407.1 " industrial crops seed the
1 part: fiber-like ", random number take grain number be 200~400, each repetition 50~100, totally 4 repetitions.
In step (2), in the casting bed of coercing cultivation, 15% Macrogol 6000 solution 15- is added in every 100 g dry sand
25mL, pH 6.0-7.5, stir evenly, and are paved into the casting bed of 1.0-1.5cm thickness in germination box, duplicate for planting experimentally by 4
Son is equably controlled respectively in the casting bed paved, with flat vessel Zhenping seed, falls into its half in sand, thereon lid one again
0.8 cm of thickness degree~1.0cm damp sand, paves applied, covers, in 20-30 DEG C of illumination box of merging, exposes sand to cotyledon
Start to carry out daylight behind face.
Preferably, the additional amount of the polyethylene glycol is 20mL, intensity of illumination >=1200lx, light application time 8h.
Further, in the above-mentioned technical solutions, in step (3), in the casting bed of control, clear water is added in every 100g dry sand
15-25ml。
Preferably, the clear water is deionized water, additional amount 20mL.
Further, in the above-mentioned technical solutions, in step (4), investigation method is that the first counting number of days of control is 4
D, it is 12 d that last, which counts number of days, and normal seedling number is counted when counting every time, extracts normal seedling at the end of statistics；Coercing cultivation
It is once counted in the 12nd d.
Preferably, the predetermined time is 12 d,
Further, in the above-mentioned technical solutions, the drought resistance of kind is judged according to the size of GI, GI value is bigger, kind
Drought resistance is stronger.
Drought tolerance classification
The classification of cotton seeds drought tolerance is as follows:
1 grade of drought-enduring coefficient >=0.90, drought tolerance are sorted out: extremely strong.
2 grade 0.75≤drought-enduring coefficient < 0.90, drought tolerance is sorted out: strong.
3 grade 0.50≤drought-enduring coefficient < 0.75, drought tolerance is sorted out: medium.
4 grades of drought-enduring coefficient < 0.50, drought tolerance are sorted out: weak.
The positive effect of the present invention is as follows:
The present invention has that environment is easy to be uniformly controlled, qualification cycle is short, easily operated, testing result is more accurate, convenient for promoting
Implement, is especially suitable for the screening of drought resistance of extensive germ plasm resource.
Specific embodiment
Following example is a further detailed description of the invention.
Embodiment 1
Step 1: preparation of samples:
From well-mixed net seed, random number takes 41 cotton seeds of nakamise 2400.
Step 2: coercing cultivation: set 10%, 15%, 20%, 25% and 30% 5 concentration gradient, 4 repetitions of each concentration,
Every repetition 100.10%, 15%, 20%, 25% and 30% polyglycol solution 20 ml, pH are separately added into every 100 g dry sand is
6.0~7.5, it stirs evenly, equably controls 4 duplicate seeds to be measured respectively in germination box paving thickness 1.5cm damp sand
Fall into its half in sand with flat vessel Zhenping seed in the casting bed paved, thereon lid a layer thickness 1.0cm damp sand again, paving
Flat wiping is even, covers, and in the illumination box of 30 DEG C ± 1 DEG C of merging, starts the light for carrying out 8 h on daytime after cotyledon exposes sand face
According to intensity of illumination >=1200lx.
Step 3: control culture: 4 repetitions, every repetition 100.20 ml of deionized water, pH are added in every 100 g dry sand
It is 6.0~7.5, stirs evenly, equably puts 4 duplicate seeds to be measured respectively in germination box paving thickness 1.5cm damp sand
It is distributed in the casting bed paved, with flat vessel Zhenping seed, falls into its half in sand, 1.0 cm of lid a layer thickness is wet again thereon
Sand, paves applied, covers, in 30 DEG C ± 1 DEG C of illumination box of merging, starts to carry out daytime 8 after cotyledon exposes sand face
The illumination of h, intensity of illumination >=1200 lx.
Step 4: investigation statistics: the first counting number of days for compareing culture is 4 d, and it is 12 d that last, which counts number of days, is counted every time
Normal seedling number is counted when number, extracts normal seedling at the end of statistics；Coercing cultivation counts normal seedling number in the 12nd d.
To the germinative number investigation statistics result such as table 1 of embodiment 1:
1 various concentration polyethylene glycol of table and the Cotton Seed rate of control
The result shows that (table 1), germination percentage of the cotton seeds under different in moisture stress is totally on a declining curve.In moderate water
Germination percentage only declines 7.2% compared with the control under stress (10%), and the two difference does not reach the level of signifiance.With the reduction of the flow of water, respectively
Processing difference reaches extremely significant level.When PEG concentration is increased to 15%, germination percentage decline 30.9%；When PEG concentration is 20%, only
(9), only a few seed germinations, germination percentage decline 90.7%；Germination is totally constrained when PEG concentration reaches 25%, germination percentage
It is 0.
Step 5: result calculates:
Calculating and analysis such as table 2 to 1 drought resistance coefficient of embodiment:
The drought resistance coefficient of 2 various concentration polyethylene glycol of table processing
Wherein: A, B, C indicate extremely significant in 0.01 horizontal upper difference, and a, b, c are indicated in 0.05 horizontal upper significant difference.
The result shows that (table 2), drought resistance coefficient of three cotton varieties under various concentration salt stress is with Polyethylene glycol
Raising and reduce, but 10% polyethylene glycol arid coefficient close to control, 15% polyethylene glycol arid coefficient is only 0.09, side
Difference analysis the result shows that, three processing between drought resistance coefficient difference reach extremely significant level.
Therefore, the index of the drought resistance coefficient for using 15% polyethylene glycol to handle as Identification of Drought is more suitable.
Embodiment 2
Step 1: preparation of samples:
From well-mixed net seed, random number takes middle H177, nakamise 49, early 72 cotton seeds of nakamise 79 and new land each
800.Wherein, middle H177 be drought resisting check variety, other 3 be crop field main breed.
Step 2: coercing cultivation: 4 repetitions, every repetition 100.15% poly- second two is separately added into every 100 g dry sand
Alcoholic solution 20 ml, pH are 6.0~7.5, are stirred evenly, and thickness 1.5cm damp sand are spread in germination box, by 4 duplicate to be measured kind
Son is equably controlled respectively in the casting bed paved, with flat vessel Zhenping seed, falls into its half in sand, thereon lid one again
1.0 cm damp sand of thickness degree, paves applied, covers, in 30 DEG C ± 1 DEG C of illumination box of merging, after cotyledon exposes sand face
Start the illumination of progress 8 h on daytime.
Step 3: control culture: 4 repetitions, every repetition 100.20 ml of deionized water, pH are added in every 100 g dry sand
It is 6.0~7.5, stirs evenly, equably puts 4 duplicate seeds to be measured respectively in germination box paving thickness 1.5cm damp sand
It is distributed in the casting bed paved, with flat vessel Zhenping seed, falls into its half in sand, 1.0 cm of lid a layer thickness is wet again thereon
Sand, paves applied, covers, in 30 DEG C ± 1 DEG C of illumination box of merging, starts to carry out daytime 8 after cotyledon exposes sand face
The illumination of h.
Step 4: investigation statistics: the first counting number of days for compareing culture is 4 d, and it is 12 d that last, which counts number of days, is counted every time
Normal seedling number is counted when number, extracts normal seedling at the end of statistics；Coercing cultivation counts normal seedling number in the 12nd d.
Step 5: result calculates:
To the calculating such as table 3 of the drought resistance coefficient of embodiment 2:
3 different cultivars drought resistance coefficient of table compares
The cotton seeds drought resistance coefficient of Different Drought is significantly different it can be seen from 3 data of table, is commented using what this method obtained
Valence result, which meets, provides the seed information explanation of unit.Illustrate that this method can preferably carry out the Identification of Drought of cotton seeds and comment
Valence.
In conclusion be merely preferred embodiments of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (10)
1. a kind of method of cotton seeds Identification of Drought, it is characterised in that: using various concentration polyglycol solution to cotton
Culture is coerced and compareed to seed, is compared using drought resistance coefficient, and cotton seeds drought resistance is evaluated.
2. the method for cotton seeds Identification of Drought according to claim 1, which comprises the steps of:
(1) preparation of samples:
From well-mixed net cotton seeds, random number takes cotton seeds number；
(2) coercing cultivation:
Cotton seeds are planted into the casting bed made of certain density polyglycol solution, illumination box culture are placed in, to son
Leaf exposes the illumination of progress certain time behind sand face；
(3) control culture:
Cotton seed is planted into the casting bed culture made of clear water, is placed in illumination box culture, carries out one after cotyledon exposes sand face
The illumination fixed time；
(4) investigation statistics:
After cultivating the predetermined time, chitting piece number is investigated；
(5) result calculating and characterization and evaluation:
Calculating pats mean value everywhere, and calculates drought resistance coefficient GI as follows:
GI = GDS/GCK ×100
Wherein: GDSFor duplicate average germination percentage under drought stress processing, GCKFor average germination percentage duplicate under control treatment.
3. the method for cotton seeds Identification of Drought according to claim 2, it is characterised in that: in step (1), cotton kind
Son is the cotton seed of full seed, and seed quality meets the regulation of GB4407.1 " industrial crops seed part 1: fiber-like ", with
Machine number takes cotton seeds number 400~800, and each repetition 50~100, totally 4 repetitions.
4. the method for cotton seeds Identification of Drought according to claim 2, it is characterised in that: in step (2), poly- second two
The concentration of alcoholic solution is 15%, and in the casting bed of coercing cultivation, every 100g dry sand is added 15% polyglycol solution 15-25mL, pH and is
6.0-7.5 is stirred evenly, in the damp sand of germination box paving 1.0-1.5cm thickness, by 4 duplicate seeds to be measured, respectively equably
It controls in the casting bed paved, with flat vessel Zhenping seed, falls into its half in sand, thereon lid one layer of 0.8-1.0cm again
Damp sand, paves applied, covers, is placed in illumination box, the temperature of illumination box is 20-30 DEG C, exposes sand face to cotyledon
After start carry out illumination.
5. the method for cotton seeds Identification of Drought according to claim 4, it is characterised in that: the polyethylene glycol adds
Entering amount is 20mL, intensity of illumination >=1200lx, light application time 8h.
6. the method for cotton seeds Identification of Drought according to claim 2, which is characterized in that in step (3), control
In casting bed, clear water 15-25mL is added in every 100g dry sand.
7. the method for cotton seeds Identification of Drought according to claim 6, it is characterised in that: the clear water be go from
Son, additional amount 20mL.
8. the method for cotton seeds Identification of Drought according to claim 2, it is characterised in that: in step (4), investigation side
Method is that the first counting number of days of control is 4d, and it is 12d that last, which counts number of days, and normal seedling number, statistics knot are counted when counting every time
Normal seedling is extracted when beam；Coercing cultivation is disposably counted in 12d.
9. the method for cotton seeds Identification of Drought according to claim 2, it is characterised in that: the predetermined time is
12d。
10. the method for cotton seeds Identification of Drought according to claim 2, it is characterised in that: according to the size of GI come
Judge the drought resistance of kind, GI value is bigger, and the drought resistance of kind is stronger.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110226484A (en) * | 2019-07-23 | 2019-09-13 | 中国农业科学院棉花研究所 | A method of enhancing cotton seedling drought resistance |
| CN112400402A (en) * | 2020-11-09 | 2021-02-26 | 塔里木大学 | Method for rapidly identifying drought tolerance of cotton germplasm resources through water culture |
| CN114051788A (en) * | 2021-11-15 | 2022-02-18 | 山西农业大学农业基因资源研究中心 | Method for identifying drought resistance of soybean in sprouting period |
| CN114568297A (en) * | 2022-03-23 | 2022-06-03 | 河南省农业科学院经济作物研究所 | Drought-resistant cotton breeding method and application |
| CN114946319A (en) * | 2022-06-08 | 2022-08-30 | 中国农业科学院棉花研究所 | Method for evaluating drought resistance of upland cotton in germination period by using double-concentration PEG6000 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110226484A (en) * | 2019-07-23 | 2019-09-13 | 中国农业科学院棉花研究所 | A method of enhancing cotton seedling drought resistance |
| CN112400402A (en) * | 2020-11-09 | 2021-02-26 | 塔里木大学 | Method for rapidly identifying drought tolerance of cotton germplasm resources through water culture |
| CN114051788A (en) * | 2021-11-15 | 2022-02-18 | 山西农业大学农业基因资源研究中心 | Method for identifying drought resistance of soybean in sprouting period |
| CN114568297A (en) * | 2022-03-23 | 2022-06-03 | 河南省农业科学院经济作物研究所 | Drought-resistant cotton breeding method and application |
| CN114946319A (en) * | 2022-06-08 | 2022-08-30 | 中国农业科学院棉花研究所 | Method for evaluating drought resistance of upland cotton in germination period by using double-concentration PEG6000 |
| CN114946319B (en) * | 2022-06-08 | 2024-01-26 | 中国农业科学院棉花研究所 | Method for evaluating drought resistance of upland cotton in germination period by using double-concentration PEG6000 |
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