CN103299806A - Switchgrass cuttage vegetative propagation method - Google Patents
Switchgrass cuttage vegetative propagation method Download PDFInfo
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- CN103299806A CN103299806A CN2013102189102A CN201310218910A CN103299806A CN 103299806 A CN103299806 A CN 103299806A CN 2013102189102 A CN2013102189102 A CN 2013102189102A CN 201310218910 A CN201310218910 A CN 201310218910A CN 103299806 A CN103299806 A CN 103299806A
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Abstract
The invention discloses a switchgrass cuttage vegetative propagation method which comprises the following steps: when the switchgrass grows to period with 4-6 knobs, removing the top and leaving 3-4 knobs below the top so as to break the apical dominance and prompt the growth of axillary buds on the knobs below; when lateral buds of 15-20 cm long grow from the axillary buds, cutting the lateral buds together with the parts which are 5 cm long above and below the knobs, so as to be the cuttings; trimming the cuttings and carrying out TDZ (Thidiazuron) rooting powder treatment, and cuttaging the cuttings into a culture medium to carry out rooting culture; and transplanting to a greenhouse or a land after the rooting is accomplished. According to the switchgrass cuttage vegetative propagation method disclosed by the invention, the vegetative propagation is tried on perennial switchgrass of the grass family by using the cuttaging method, and unexpected and good breeding effect is achieved; and the method has the characteristics of being simple and feasible, high in survival rate, unlikely to have variation and the like, and is particularly applicable to providing materials for switchgrass biotechnology study.
Description
Technical field
The invention belongs to switchgrass propagation technique field, relate to a kind of switchgrass cuttage asexual reproduction method.
Background technology
Switchgrass (English name Switchgrass, latin name Panicum virgatum Linn) be the grass family herbaceos perennial, the former north America region of being born in, have the characteristics such as cultivation is simple, strong stress resistance, wide adaptability, since early 1990s, China's researcher is progressively introduced a fine variety it areas such as semiarid loess hill-valley area and Ansai, Shaanxi.Switchgrass belongs to C
4The class plant, has very high year biomass yield, mainly be used as at first forage crop and gardens crop, the eighties in 20th century, (the US Department Of Energy of american energy department, DOE) it is chosen to be the pattern energy-source plant, after this, various countries have all strengthened the research to switchgrass.
At present both at home and abroad the research of switchgrass mainly concentrated on the fields such as agricultural science, environmental science and bioenergy conversion, and at the research proportion of switchgrass biological technical field seldom.As a kind of pattern energy-source plant, switchgrass has a lot of merits, and is tall and big such as drought-enduring, impoverishment tolerant, disease and insect resistance, plant, tiller manyly etc., has good biotechnology research prospect.Yet switchgrass is a kind of cross-pollinatd plant, has very strong certainly colored incompatibility, and therefore, the offspring that sexual propagation obtains often has different genotype, and this is unfavorable for the switchgrass biotechnology research.
Vegetative propagation is the process that is directly obtained whole plant by the organ of plant, owing to do not relate to Gene Isolation and genetic recombination in this process, so the offspring plant of gained has identical genotype.At present, the switchgrass asexual reproduction method has by callus plantlet with by the induced bundle Fast-propagation of sprouting.The tradition cycle is long, and has certain probability to produce variation in Induction Process, therefore provides material improper take callus induction regrowth method as biotechnology research.
Cuttage is a kind of comparatively ripe vegetative propagation technique, but is usually used in dicotyledon, also only has Liliaceae Asparagus and Commelianaceae Activities of Some Plants to utilize the cottage propagation mode in the monocotyledon, and seldom finds to use in grass family.
Summary of the invention
The problem that the present invention solves is to provide a kind of switchgrass cuttage asexual reproduction method, and the method is a kind of easy switchgrass propagation method, is conducive to obtain the consistent progeny material of genotype.
The present invention is achieved through the following technical solutions:
A kind of switchgrass cuttage asexual reproduction method may further comprise the steps:
1) after switchgrass grows 4～6 joints, remove and tiller the top and keep 3～4 joints in below, axillalry bud is germinated.Tiller after the top cut 20～30 days, axillary bud growth becomes the long lateral bud of 15～20cm;
2) with lateral bud together with its place joint and joint up and down each 3～5cm cut from the switchgrass plant, as cutting; Peel off and transplant a cutting the blade of parcel lateral bud on the base portion maternal plant, and will transplant a cutting the lower end and be whittled into wedge shape;
3) the cutting bottom after will pruning is immersed in the rooting powder solution more than the 12h, then will transplant a cutting and insert in the culturing pot that loose permeable culture matrix is housed, and culturing pot is placed water, makes culture matrix begin immersion from the bottom, until the culture matrix suction is saturated; Then culturing pot is placed in the constant temperature greenhouse and cultivates, treat that new root grows to 10～20cm long;
4) cutting of having taken root in the culturing pot is taken out, transplant in the greenhouse.
The described removal top of tillering is in 7～August, treats that switchgrass grows 4～6 fully internodes of elongation, and the individual plant of the switchgrass of selected robust growth is removed and tillered the top and keep 3～4 joints in below.
Described until lateral bud is cut as cutting from switchgrass after, the axillalry bud that the below of lateral bud place joint is close on the joint germinates, and after this axillary bud growth to 15～20cm is long, it is carried out second as cutting take turns cuttage.
Described root-inducing powder is TDZ root-inducing powder (TDZ, Thidiazuron, the phenyl thiadiazolyl group urea of 500～1000 times of dilutions; High bio tech ltd is matched in Shaanxi, No. 9, instrument road, Xi'an new and high-tech development zone east, postcode: 710021), carry out cuttage after rooting powder solution soaks 24h.
Described culture matrix is that 1～2 part of cultivating soil, 4～6 parts of leaf moulds and 1～2 part of abundant mixing of river sand are obtained.
It is to cultivate 6～8 days in 20～25 ℃, the constant temperature greenhouse of 14h illumination every day that described culturing pot is placed to temperature.
The kind of described switchgrass is that the U.S. introduces cultivar Alamo.
Compared with prior art, the present invention has following useful technique effect:
Switchgrass cuttage asexual reproduction method provided by the invention attempts adopting cottage method that the perennial switchgrass of grass family is carried out vegetative propagation, has obtained unexpected reproductive effect; And in reproductive process, do not relate to tissue culture procedures, compare with traditional callus induction differentiation and regeneration plant and inducing clumping bud Fast-propagation have simple, survival rate is high, and the characteristics such as be difficult for morphing are very suitable for providing material for the switchgrass biotechnology research.
Switchgrass cuttage asexual reproduction method provided by the invention, incubation is simple:
No matter be that callus induction regeneration plant Fast-propagation or inducing clumping bud Fast-propagation all relate to tissue culture procedures, need to explant carry out disinfection and be inoculated on the dissimilar medium induce, regenerate, take root, the series of steps such as hardening, transplanting.It is all strict that tissue is cultivated each process, and in the process of cultivating, often relate to the FAQs such as pollution, albefaction, somaclonal variation, and the present invention adopts cottage propagation, the loaded down with trivial details step such as removed the sterilization in the tissue culture procedures from, induce, and each step is compared all very simple with the tissue cultivation, the cutting of taking root after finishing can be directly used in transplanting.
Switchgrass cuttage asexual reproduction method provided by the invention, equipment is simple, survival rate is high:
In this switchgrass asexual reproduction method fulfillment process, do not relate to complicated experimental facilities, under greenhouse experiment, can carry out, required only is cuttage common tool, material and commercially available root-inducing powder etc., and condition is controlled survival rate very high (the cuttage root-taking survival rate is 100% in concrete experiment) in the good situation.
Switchgrass cuttage asexual reproduction method provided by the invention, can repeatedly carry out in 1 year:
7～August, switchgrass grows 4～6 fully internodes of elongation, cutting switchgrass removal apical dominance can carry out at twice, for the first time harvesting stays 3～4 joints with switchgrass, the axillalry bud that this moment, left top was saved germinates, and the axillalry bud of below still is in and is subjected to holddown, about 30 days, it is long that uppermost axillalry bud can grow into 15～20cm, can cut as transplanting a cutting, after cutting is cut, the axillalry bud that the below is close on the joint germinates, the lateral bud that grows can be done second and take turns cuttage, so, can further improve and expands numerous efficient.
Switchgrass cuttage asexual reproduction method provided by the invention can enlarge rapidly a certain genotype plant colony:
Because whole process does not relate to chromosomal separation and restructuring, therefore, resulting offspring's genotype and parental plant genotype are in full accord.Simultaneously, the method can also be used for the quick expanding propagation by the transfer-gen plant of animal nutrition acquisition.
Switchgrass cuttage asexual reproduction method provided by the invention does not use complicated experimental instrument and equipment, and medicine, instrument and material are all very simple, compare with original method, greatly reduce production cost.
Description of drawings
Fig. 1 is cottage propagation schematic flow sheet of the present invention;
Fig. 2 is the cutting schematic diagram after pruning;
Fig. 3 is the switchgrass schematic diagram after taking root.
Embodiment
Switchgrass cuttage asexual reproduction method provided by the invention, pilot production is carried out vegetative propagation with cottage method to the perennial switchgrass of grass family, has obtained good reproductive effect, has simplely, and survival rate is high, and the characteristics such as be difficult for morphing.The present invention is described in further detail below in conjunction with specific embodiment, and the explanation of the invention is not limited.
Referring to Fig. 1, a kind of switchgrass cuttage asexual reproduction method, comprise axillary bud development induce, transplant a cutting draw materials, clip cutting and processing, rooting of cuttings cultivation, intermediate house etc., step is as follows:
1) after switchgrass grows 4～6 joints, remove and tiller the top and keep 3～4 joints in below, axillalry bud is germinated.After the top of tillering was cut 20～30 days, axillary bud growth became the long lateral bud of 15～20cm;
2) with lateral bud together with its place joint and up and down each 3～5cm cut from the switchgrass plant, as cutting; Peel off and transplant a cutting the blade of parcel lateral bud on the base portion maternal plant, and will transplant a cutting the lower end and be whittled into wedge shape;
Concrete, the cutting after the pruning is as shown in Figure 2;
3) the cutting bottom after will pruning is immersed in the rooting powder solution at least 12h, then will transplant a cutting and insert in the culturing pot that loose permeable culture matrix is housed, and culturing pot is placed water, makes culture matrix begin immersion from the bottom, until the culture matrix suction is saturated; Then culturing pot is placed in the constant temperature greenhouse and cultivates, treat that new root grows to 10～20cm long;
Switchgrass after concrete the taking root as shown in Figure 3;
4) cutting of having taken root in the culturing pot is taken out, transplant in the greenhouse.Under the greenhouse constant temperature, the cuttage seeding survival rate can reach 100%, can grow then 5～10 and tiller; Second Year moves to outdoor, can obtain the plant of normal growth, and the Second Year seedling of growing way and seminal propagation is suitable.
Further, concrete operation may further comprise the steps:
(1) remove apical dominance, induce axillary bud development:
A. for guaranteeing that switchgrass has high vigor and guarantees sufficient energy supply, sampling time of explant is defined as switchgrass and grows 4～6 joints and be advisable.
B. remove the top of tillering, keep 3～4 joints in below, break the switchgrass apical dominance, axillalry bud germinates.
(2) culture matrix is prepared:
Culture matrix adopts loose permeable material, is beneficial to transplant a cutting the growth of root.The flower culturing soil of used matrix for buying in this method, composition is 2 parts of cultivating soils (garden mould), 6 parts of leaf moulds, 2 parts of river sands.
(3) draw materials:
A. after the switchgrass top was cut 20～30 days, the top axillalry bud can grow into the long lateral bud of 15～20cm.
B. with lateral bud its together with its place joint up and down each 3～5cm cut from plant.
(4) cutting is processed:
A. peel off to transplant a cutting and be wrapped in the blade of lateral bud on the base portion maternal plant, and will transplant a cutting the lower end and be whittled into wedge shape, so that cuttage.
B. it is 24h in 0.1% the TDZ rooting powder solution that the cutting bottom after will pruning is immersed in mass concentration.
(5) cuttage is cultivated:
A. the cutting after will processing is inserted and is equipped with in the culturing pot of culture matrix, and culturing pot places in the basin, and adds water in basin, makes culture matrix begin immersion from the bottom, until it is saturated to absorb water.
B. culturing pot is placed in 20 ℃ of constant temperature greenhouses and cultivates, about a week, it is long that new root can grow to 10～20cm.
(6) transplant:
The cutting of having taken root in the culturing pot is taken out, transplant in the greenhouse, so far, transplanting a cutting can normal growth.
The below introduces cultivation take this kind of switchgrass cultivar Alamo(by the U.S.) individual plant carries out fast-propagation and is described in detail as specific embodiment.
Embodiment 1:
The cuttage asexual reproduction method of switchgrass cultivar Alamo comprises following operation:
(1) removes apical dominance, induce axillary bud development
A.8 at the beginning of the moon, select individual plant of switchgrass kind Alamo of robust growth.
B. switchgrass is cut apart from ground 50～60cm eminence, stayed 3～4 joints in below.
(2) culture matrix is prepared
Buy the flower culturing soil culture matrix from flower shed, composition is 2 parts of cultivating soils (garden mould), 6 parts of leaf moulds, 2 parts of river sands.
(3) draw materials
A. after the switchgrass top was cut 20～30 days, the top axillalry bud can grow into the long lateral bud of 15～20cm.
B. with lateral bud its together with its place joint up and down each 3～5cm cut from plant.
(4) cutting is processed
A. peel off to transplant a cutting and be wrapped in the blade of lateral bud on the base portion maternal plant, and will transplant a cutting the lower end and be whittled into wedge shape, so that cuttage.
B. it is 24h in 0.1% the TDZ rooting powder solution that the cutting bottom after will pruning is immersed in mass concentration.
(5): cuttage is cultivated
A. the cutting after will processing is inserted and is equipped with in the culturing pot of culture matrix, and culturing pot places in the basin, and adds water in basin, makes culture matrix begin immersion from the bottom, until it is saturated to absorb water.
B. culturing pot is placed in 20 ℃ of constant temperature greenhouses and cultivates, about a week, it is long that new root can grow to 10～20cm.
(6): transplant
The cutting of having taken root in the culturing pot is taken out, and transplant again that off-the-shelf bore is in the large flowerpot of 25cm in the greenhouse, so far, cutting can normal growth.
Claims (7)
1. a switchgrass cuttage asexual reproduction method is characterized in that, may further comprise the steps:
1) after switchgrass grows 4～6 joints, remove and tiller the top and keep 3～4 joints in below, impel axillalry bud to germinate, after the top of tillering was cut 20～30 days, axillary bud growth became the long lateral bud of 15～20cm;
2) with lateral bud together with its place joint and joint up and down each 3～5cm cut from the switchgrass plant, as cutting; Peel off and transplant a cutting the blade of parcel lateral bud on the base portion maternal plant, and will transplant a cutting the lower end and be whittled into wedge shape;
3) the cutting bottom after will pruning is immersed in the rooting powder solution more than the 12h, then will transplant a cutting and insert in the culturing pot that loose permeable culture matrix is housed, and culturing pot is placed water, makes culture matrix begin immersion from the bottom, until the culture matrix suction is saturated; Then culturing pot is placed in the constant temperature greenhouse and cultivates, treat that new root grows to 10～20cm long;
4) cutting of having taken root in the culturing pot is taken out, transplant in the greenhouse.
2. switchgrass cuttage asexual reproduction method as claimed in claim 1, it is characterized in that, the described removal top of tillering is in 7～August, treats that switchgrass grows 4～6 fully internodes of elongation, the individual plant of the switchgrass of selected robust growth is except 3～4 joints in top and reservation below of tillering.
3. switchgrass cuttage asexual reproduction method as claimed in claim 1, it is characterized in that, cut from switchgrass as after transplanting a cutting until lateral bud, the axillalry bud that the below of lateral bud place joint is close on the joint germinates, after this axillary bud growth to 15～20cm is long, it is carried out second as cutting take turns cuttage.
4. switchgrass cuttage asexual reproduction method as claimed in claim 1 is characterized in that, described root-inducing powder is the TDZ root-inducing powder of 500～1000 times of dilutions, carries out cuttage after rooting powder solution soaks 24h.
5. switchgrass cuttage asexual reproduction method as claimed in claim 1 is characterized in that, in volume parts, described culture matrix is that 1～2 part of cultivating soil, 4～6 parts of leaf moulds and 1～2 part of abundant mixing of river sand are obtained.
6. switchgrass cuttage asexual reproduction method as claimed in claim 1 is characterized in that, it is to cultivate 6～8 days in 20～25 ℃ the constant temperature greenhouse that described culturing pot is placed to temperature.
7. switchgrass cuttage asexual reproduction method as claimed in claim 1 is characterized in that, the kind of described switchgrass is Alamo.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104041269A (en) * | 2014-05-12 | 2014-09-17 | 宁夏大学 | Method for quickly planting fracturing alkali soil switchgrass |
| CN104115651A (en) * | 2014-07-04 | 2014-10-29 | 中国科学院东北地理与农业生态研究所 | Rapid propagation method for cuttage of salix brachypoda |
| CN105052500A (en) * | 2015-08-20 | 2015-11-18 | 中国科学院东北地理与农业生态研究所 | Method for rapid propagation of switchgrass through rhizome transplantation |
| CN107006259A (en) * | 2017-04-28 | 2017-08-04 | 凤冈县领益昕中药材种植有限公司 | The mating system of lucid asparagus |
| CN109005973A (en) * | 2018-08-27 | 2018-12-18 | 山东省林业科学研究院 | A method of improving dryland willow cutting plantation |
| CN115462303A (en) * | 2022-09-06 | 2022-12-13 | 北京恒丰亚业科技发展有限公司 | Seedling raising method for gramineous plants |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946617A (en) * | 2010-08-23 | 2011-01-19 | 湖南农业大学 | Cottage and rapid propagation method of perennial energy grass |
-
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946617A (en) * | 2010-08-23 | 2011-01-19 | 湖南农业大学 | Cottage and rapid propagation method of perennial energy grass |
Non-Patent Citations (2)
| Title |
|---|
| 朱毅: "两种沙性栽培基质下柳枝稷根系生长对施氮水平的响应", 《中国草地学报》 * |
| 黄楠: "能源作物柳枝稷栽培技术", 《现代农业科技》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104041269A (en) * | 2014-05-12 | 2014-09-17 | 宁夏大学 | Method for quickly planting fracturing alkali soil switchgrass |
| CN104115651A (en) * | 2014-07-04 | 2014-10-29 | 中国科学院东北地理与农业生态研究所 | Rapid propagation method for cuttage of salix brachypoda |
| CN105052500A (en) * | 2015-08-20 | 2015-11-18 | 中国科学院东北地理与农业生态研究所 | Method for rapid propagation of switchgrass through rhizome transplantation |
| CN107006259A (en) * | 2017-04-28 | 2017-08-04 | 凤冈县领益昕中药材种植有限公司 | The mating system of lucid asparagus |
| CN109005973A (en) * | 2018-08-27 | 2018-12-18 | 山东省林业科学研究院 | A method of improving dryland willow cutting plantation |
| CN115462303A (en) * | 2022-09-06 | 2022-12-13 | 北京恒丰亚业科技发展有限公司 | Seedling raising method for gramineous plants |
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