CN103444536B - Method for reducing tissue culture production cost of hosta plantagineu - Google Patents
Method for reducing tissue culture production cost of hosta plantagineu Download PDFInfo
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- CN103444536B CN103444536B CN201310402633.0A CN201310402633A CN103444536B CN 103444536 B CN103444536 B CN 103444536B CN 201310402633 A CN201310402633 A CN 201310402633A CN 103444536 B CN103444536 B CN 103444536B
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Abstract
The invention discloses a method for reducing tissue culture production cost of hosta plantagineu. The method comprises the steps: obtaining a tissue block with an adventitious bud, differentiating and multiplying, rooting, transplanting, acclimatizing, planting in a large land, and the like. According to the method, through screening a proper culture medium and selecting a proper culturing and transplanting method, the culture efficiency of the hosta plantagineu is improved, and the production cost is lowered.
Description
Technical field
The present invention relates to plant tissue culture technical field, specifically a kind of method reducing floral leaf jade hairpin group training production cost.
Technical background
Floral leaf jade hairpin (
hosta sieboldiana ) be Liliaceae perennial root herbage flower, be in recent years from the external jade hairpin new varieties introduced.The growth of floral leaf jade hairpin is strong, resists cold and happiness the moon, avoids direct sunlight, plant or building north side well-grown lower in tree.Floral leaf jade hairpin not only has the good characteristic of jade hairpin, and leaf color is distinct, secondary color blade face colour bar is clear, and vein train of thought is obvious, is very excellent potted plant and greening kind.
Floral leaf jade hairpin, by the impact of introducing the factors such as quantity is few, plant division speed is slow, limits its application speed.Tissue cultures can keep the merit of kind and the effective way of rapid scale production, but production cost is relatively high.Rooting of vitro seedling and transplanting stage adopt the mode of simple bud to carry out taking root and transplanting usually.
Summary of the invention
The object of this invention is to provide a kind of method reducing floral leaf jade hairpin group training production cost, the method culture efficiency is high, and cost is low, is suitable for factorial praluction.
The technical solution used in the present invention:
Reduce a method for floral leaf jade hairpin group training production cost, comprise the steps:
(1) obtain the tissue block of band indefinite bud: the young shoot getting floral leaf jade hairpin spring, the fritter being cut into 1cm after sterilization long is inoculated on bud inducement medium, cultivates the tissue block that 30d obtains being with indefinite bud;
(2) differentiation and proliferation: be seeded on inductive differentiation medium by the tissue block of band indefinite bud, squamous subculture 25 ~ 30d, must grow thickly sprout tuber;
(3) take root: the sprout tuber that will grow thickly cuts into 1cm
3fritter, be directly inoculated on root media, cultivate 25d, obtain rooting tube plantlet;
(4) domestication is transplanted: adopt vermiculite to be hardening matrix, use 0.1%KMno
4for subsequent use after solution disinfection, directly plant to hardening matrix by after test-tube plantlet according to a conventional method cleaning and sterilizing in the mode of Multiple Buds, keep temperature 23 ~ 25 DEG C, relative air humidity more than 80%, soil humidity 50 ~ 60%, light application time 12 ~ 14hd
-1, intensity of illumination 1000 ~ 2000Lux, hardening 30 ~ 40d;
(5) field planting land for growing field crops: carry out field-transplanting in the mode of Multiple Buds after hardening terminates, every clump of 4 ~ 5 buds, seeding row spacing 10 cm × 20cm, the spring and summer of next year carries out secondary plant division field planting again, the seeding row spacing 20cm × 30cm of secondary field planting;
Described bud inducement medium is 1/3MS+6-BA3 ~ 5mg.L
-1+ IBA 0.1 ~ 0.5 mg.L
-1;
Described differentiation and proliferation medium is MS+6-BA2.0 ~ 4.0mg.L
-1+ IBA0.1 ~ 0.5 mg.L
-1;
Described root media is MS or 1/2MS+IBA0 ~ 0.5 mg.L
-1;
Described three kinds of medium all add white granulated sugar 30 ~ 45 g.L
-1, agar 4 ~ 6 g.L
-1, pH5.8 ~ 6.0; Cultivation temperature 23 ~ 25 DEG C, light application time 12 ~ 14hd
-1, intensity of illumination 1000 ~ 2000Lux.
The present invention has carried out in the differentiation of floral leaf jade hairpin test-tube plantlet, culture of rootage stage the research that formula is cultivated in a large amount of combination, found that certain density hormone combinations proportioning is taken root have good effect to floral leaf jade hairpin test-tube plantlet differentiation, Multiple Buds.Multiple Buds rooting technique improves the cyclic utilization rate in culture vessel and culturing room space.In the test-tube plantlet acclimatization and transplants stage, simple bud and Multiple Buds are transplanted and compares, found that test-tube plantlet is not only unaffected with the growth of the mode acclimatization and transplants of Multiple Buds, and improve transplanting survival rate because of the effect of population effect, save greenhouse space and a large amount of matrix.Multiple Buds secondary field planting method, compared with a field planting method, not only increase transplanting survival rate, and field planting First Year can save the soil of 5 ~ 6 times, greatly save water, the daily management recruitment such as to hoe up weeds, reduce production management cost.Technique technique is simple, and cultivation efficiency is high, significantly reduces production cost.
Embodiment
The present invention is described in detail to the inventive method for the rapid seedling cultivation in floral leaf jade hairpin ' France '.
Embodiment 1
(1) get the young shoot of floral leaf jade hairpin spring, remove partial blade and root system, after tap water is clean on workbench, with alcohol-pickled 30s, the 0.1%HgCl of 75%
2soak 10min, aseptic water washing 4 ~ 5 times, is cut into about 1cm and is inoculated in bud inducement medium 1/3MS+6-BA 3mg.L by budlet
-1+ IBA 0.1 mg.L
-1+ white granulated sugar 30 g.L
-1+ agar 4 g.L
-1on, pH5.8 ~ 6.0, cultivation temperature 23 ~ 25 DEG C, light application time 12 ~ 14hd
-1, intensity of illumination 1000 ~ 2000Lux, cultivates the tissue block that 30d obtains being with indefinite bud.
(2) be 1cm by size
3the band indefinite bud tissue block of left and right is seeded in inductive differentiation medium MS+6-BA 2.0mg.L
-1+ IBA 0.1mg.L
-1+ white granulated sugar 30 g.L
-1+ agar 4 g.L
-1on, pH5.8 ~ 6.0, its condition of culture is with (1).Each experimental group inoculates 5 pieces, establishes 10 experimental group altogether, and after 25d, the newly-increased sprout tuber of statistics, the results are shown in Table 1.
Table 1: floral leaf jade hairpin ' France ' plantlet in vitro differentiation result
(3) sprout tuber that will grow thickly cuts into 1cm
3the fritter of left and right, is directly inoculated in root media 1/2MS+IBA 0.3 mg.L
-1+ white granulated sugar 35 g.L
-1+ agar 6 g.L
-1on, PH5.8 ~ 6.0, its condition of culture is with (1).Each experimental group inoculates 5 pieces, and establish 8 experimental group altogether, after 25d, statistics is in table 2.
Table 2: floral leaf jade hairpin ' France ' plantlet in vitro Multiple Buds rooting
(4) adopt vermiculite to be hardening matrix, use 0.1%KMno
4for subsequent use after solution disinfection, directly plant to hardening matrix by after test-tube plantlet according to a conventional method cleaning and sterilizing in the mode of Multiple Buds, keep temperature 23 ~ 25 DEG C, relative air humidity more than 80%, soil humidity 50 ~ 60%, light application time 12 ~ 14hd
-1, intensity of illumination 1000 ~ 2000Lux, hardening 35d;
(5) hardening carries out field-transplanting in the mode of Multiple Buds after terminating, every clump of 4 ~ 5 buds, seeding row spacing 10 cm × 20cm, and the spring and summer of next year carries out secondary plant division field planting again, the seeding row spacing 20cm × 30cm of secondary field planting.
The cost compare of the inventive method and Sterile culture method is in table 3.
The cost compare of table 3 Multiple Buds formula propagation method and Sterile culture method
Note: Cost accounting method with 100L medium (1500 bottles) preparation, inoculation, cultivate needed for medicine, electricity consumption, recruitment calculate, do not comprise the depreciation of vessel and instrument and equipment, factory building.Multiple Buds field planting cost is 1 mu of ground management cost of 2 years.The height of this table just 2 kinds of method costs compares, and is not final individual plant production cost.
Adopt the inventive method to cultivate floral leaf jade hairpin, the rooting rate of Multiple Buds is high, on average can reach 95%, and cultivates cost all have obvious reduction on links, and it is high that visible the method cultivates efficiency, and cost is low, is suitable for factorial praluction.
Embodiment 2
The present embodiment bud inducement medium used is 1/3MS+6-BA4mg.L
-1+ IBA 0.3 mg.L
-1+ white granulated sugar 40 g.L
-1+ agar 5 g.L
-1, differentiation and proliferation medium is MS+6-BA 3.0mg.L
-1+ IBA 0.3 mg.L
-1+ white granulated sugar 45 g.L
-1+ agar 5 g.L
-1, pH5.8 ~ 6.0; Root media used is MS or 1/2MS+IBA0 ~ 0.5 mg.L
-1+ white granulated sugar 40 g.L
-1+ agar 4 g.L
-1, pH5.8 ~ 6.0, hardening 30d in hardening matrix, other conditions are with embodiment 1, and in its differentiation step, average value-added coefficient is 4.10, and average rooting rate 96.7% in step of taking root, number of on average taking root is 7.58, and each stepped cost is suitable with embodiment 1.
Embodiment 3
The present embodiment bud inducement medium used is 1/3MS+6-BA5mg.L
-1+ IBA 0.5 mg.L
-1+ white granulated sugar 45 g.L
-1+ agar 6 g.L
-1, differentiation and proliferation medium is MS+6-BA 4.0mg.L
-1+ IBA 0.5 mg.L
-1+ white granulated sugar 45 g.L
-1+ agar 5 g.L
-1, pH5.8 ~ 6.0; Root media used is MS, hardening 40d in hardening matrix, and other conditions are with embodiment 1, in its differentiation step, average value-added coefficient is 4.08, average rooting rate 94.8% in step of taking root, number of on average taking root is 7.50, and each stepped cost is suitable with embodiment 1.
Claims (1)
1. reduce a method for floral leaf jade hairpin group training production cost, it is characterized in that comprising the steps:
(1) obtain the tissue block of band indefinite bud: the young shoot getting floral leaf jade hairpin spring, the fritter being cut into 1cm after sterilization long is inoculated on bud inducement medium, cultivates the tissue block that 30d obtains being with indefinite bud;
(2) differentiation and proliferation: be seeded on inductive differentiation medium by the tissue block of band indefinite bud, squamous subculture 25 ~ 30d, must grow thickly sprout tuber;
(3) take root: the sprout tuber that will grow thickly cuts into 1cm
3fritter, be directly inoculated on root media, cultivate 25d, obtain rooting tube plantlet;
(4) domestication is transplanted: adopt vermiculite to be hardening matrix, use 0.1%KMno
4for subsequent use after solution disinfection, directly plant to hardening matrix by after test-tube plantlet according to a conventional method cleaning and sterilizing in the mode of Multiple Buds, keep temperature 23 ~ 25 DEG C, relative air humidity more than 80%, soil humidity 50 ~ 60%, light application time 12 ~ 14hd
-1, intensity of illumination 1000 ~ 2000 Lux, hardening 30 ~ 40d;
(5) field planting land for growing field crops: carry out field-transplanting in the mode of Multiple Buds after hardening terminates, every clump of 4 ~ 5 buds, seeding row spacing 10 cm × 20cm, the spring and summer of next year carries out secondary plant division field planting again, the seeding row spacing 20cm × 30cm of secondary field planting;
Described bud inducement medium is 1/3MS+6-BA3 ~ 5mg.L
-1+ IBA 0.1 ~ 0.5 mg.L
-1;
Described inductive differentiation medium is MS+6-BA2.0 ~ 4.0mg.L
-1+ IBA0.1 ~ 0.5 mg.L
-1;
Described root media is MS or 1/2MS+IBA0 ~ 0.5 mg.L
-1;
Described three kinds of medium all also add white granulated sugar 30 ~ 45 g.L
-1, agar 4 ~ 6 g.L
-1, pH5.8 ~ 6.0; Cultivation temperature 23 ~ 25 DEG C, light application time 12 ~ 14hd
-1, intensity of illumination 1000 ~ 2000Lux.
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CN103858770B (en) * | 2014-04-03 | 2016-05-04 | 中国科学院植物研究所 | A kind of method of floral leaf jade hairpin Fast-propagation |
CN103875535B (en) * | 2014-04-03 | 2015-09-02 | 中国科学院植物研究所 | A kind of method of floral leaf jade hairpin fast breeding |
CN106613994A (en) * | 2017-01-19 | 2017-05-10 | 上海市农业科学院 | Method for acquiring fragrant plantain lily herb rooting seedlings by LED (light-emitting diode) light sources |
CN110972945A (en) * | 2019-12-19 | 2020-04-10 | 美尚生态景观股份有限公司 | Culture medium and culture method for tissue culture of plantain plantaginea |
CN111034618B (en) * | 2020-01-14 | 2021-09-03 | 浙江海丰花卉有限公司 | Culture method for flowering in camellia balsamine bottle and special culture medium thereof |
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KR20070036841A (en) * | 2005-09-30 | 2007-04-04 | 주식회사운화 | Hosta masspropagation by thin cell layer technique |
CN102273405B (en) * | 2010-06-10 | 2013-07-24 | 上海上房园艺有限公司 | Cultivating method of Hosta plantaginea Gold Standard |
CN102860261A (en) * | 2012-10-11 | 2013-01-09 | 山东鑫秋种业科技有限公司 | Method for preparing tissue culture medium of hosta undulata baileys |
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