CN105900683A - Method for cultivating edible mushrooms with floating bed on water surface of pond - Google Patents

Method for cultivating edible mushrooms with floating bed on water surface of pond Download PDF

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Publication number
CN105900683A
CN105900683A CN201610247014.2A CN201610247014A CN105900683A CN 105900683 A CN105900683 A CN 105900683A CN 201610247014 A CN201610247014 A CN 201610247014A CN 105900683 A CN105900683 A CN 105900683A
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China
Prior art keywords
bacterium bag
pond
water
floating bed
mushroom
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CN201610247014.2A
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Chinese (zh)
Inventor
邬达敏
曾柳根
张东萍
刘昌林
洪香娇
姚毅
刘辉
蓝岚
许亮清
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NANCHANG ACADEMY OF AGRICULTURAL SCIENCE
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NANCHANG ACADEMY OF AGRICULTURAL SCIENCE
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Priority to CN201610247014.2A priority Critical patent/CN105900683A/en
Publication of CN105900683A publication Critical patent/CN105900683A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • A01G31/02Special apparatus therefor
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

Abstract

The invention relates to a method for cultivating edible mushrooms with a floating bed on the water surface of a pond. Edible mushrooms traditionally cultivated indoors or on land can be cultured on the water surface of an outdoor pond, building input for a mushroom house or mushroom shed is omitted, the production cost is reduced, the economic benefits are improved, the aquaculture industry and the edible mushroom planting industry can be organically combined, and efficient, environment-friendly and ecological circulation agriculture can be formed. The edible mushroom floating bed is designed and manufactured by the inventor and can meet the requirement of various edible mushrooms, especially hygrophilous edible mushrooms such as lentinula edodes, oyster mushrooms and auricularia auricular for pond water surface cultivation; by means of pond water surface cultivation, rich nutrition and a good ecological environment are provided for growth of edible mushrooms due to the eutrophication and high humidity of the pond, and it is possible to achieve a high yield and high efficiency of edible mushrooms; meanwhile, the water quality of the pond is improved by edible mushroom fungus bags, and mushrooms generated by the fungus bags then serve as fish feed, so that aquaculture and planting of edible mushrooms are in mutual supplementation and mutual promotion, and positive significance is achieved on forming good and environment-friendly ecological circulation economy.

Description

A kind of method of pond water edema over the face bed culturing edible fungus
Technical field
The present invention relates to a kind of fungus growing technique, a kind of method of pond water edema over the face bed culturing edible fungus.
Background technology
According to our understanding to the biological characteristics of edible fungi, associate aquiculture water quality eutrophication, containing big in water quality Nutritional labeling required for the material edible fungi growths just such as amount ammonia nitrogen, Organic substance and mineral element, and most edible fungi Like the growing environment of high humility, mushroom body formed the phase require air humidity more than 95%, particularly Auricularia peace mushrooms mushroom, It addition, mycelia the highest can the bearing in long-term water of kink density is soaked after edible fungi has wrapped in physiological maturity, and complete whole Growth cycle, and edible fungi wraps in Later growth often due to hydropenia and the underproduction significantly, particularly Lentinus Edodes and Pleurotus ostreatus in bacterium bag, For this biological characteristics of edible fungi, we make use of floating bed as carrier, edible in indoor or land cultivation for a long time Bacterium has been displaced to the pond water surface and has cultivated, and edible fungi is succeeded at pond water-surface cultivation, and after solving bacterium bag The problem of the phase hydropenia underproduction, and bacterium bag is to the absorption of eutrophy composition in pond water quality so that edible fungi water-surface cultivation is than routine Cultivation has the effect of higher yield.A series of correlation test, edible fungi bag was We conducted before completing this technology Soak test, the floating bed cultivation contrast test of edible fungi different cultivars and edible fungi Pleurotus ostreatus class to pond water quality ammonia nitrogen and organic Absorption Degrading experiment of phosphorus etc., test proves that pond floating bed cultivation edible fungi has positive role and significant economic benefit, I At present design and use edible fungi floating bed at home and abroad or first by, the particularly application of this technology makes edible fungi exist Pond floating cultivation is possibly realized.
Summary of the invention
In place of the deficiencies in the prior art, the present invention provides a kind of method of pond water edema over the face bed culturing edible fungus.
For achieving the above object, technical solution of the present invention is: a kind of method of pond water edema over the face bed culturing edible fungus, including following Step:
(1) substrate prepares: selecting formula according to different edible fungus varieties, Pleurotus ostreatus class uses cotton seed hulls 60-80wt%, bagasse 10-15wt%, corn cob 5-10wt%, calcium carbonate 2-5wt%, calcium superphosphate 2-5wt%, sodium selenite 1-2wt%, quick lime 1-2%, its summation is 100%, pH value 8.0;Auricularia, Lentinus Edodes or Ganoderma use cotton seed hulls 50-60wt%, bagasse 15-25wt%, Corn cob 10-15wt%, Testa oryzae 5-10wt%, calcium carbonate 2-5wt%, calcium superphosphate 2-5wt%, sodium selenite 2-5wt% are raw Calx 1-2%, its summation is 100%;
(2) substrate fermentation, pack, sterilizing, cooling, inoculation, inoculation is all bacterium Bao Yitou inoculation, and fruiting is the most all one and erects Vertical fruiting, is different from the conventional two that produces and inoculates, and two accumbency fruiting, this is also that water-surface cultivation mode determines;
(3) floating bed is made: floating bed is polygonized structure, including chassis and the baffle plate of chassis periphery of placement bacterium bag, between baffle plate Interconnecting, the height of baffle plate can be adjusted according to the size of bacterium bag, chassis is equipped with a net torr by bacterium bag and chassis Isolation increases breathability, and bacterium bag is directly placed in net torr, has the hole that moisturizes of ellipse, upper in floating bed on baffle plate Portion adds a cover blue guard, and guard half ventilation state makes that sporophore growth is the most lopsided ensures again certain humidity, and blue guard makes Sporophore accepts intensity of illumination 200~1000lx, and wavelength is 0.4~0.5 μm;
(4) bacterium bag and floating bed are placed, that the bacterium bag covering with mycelia is opened inoculation, and plastic bag is also turned over by cleaning surface irregularities Down to bacterium bag surface, bacterium bag bottom plastic bag cuts off 1/5, makes bacterium bag mycelia directly can contact with pond water, and erects placement floating bed Centre, builds blue guard, the most sliceable various figures of floating bed above;
(5) mushroom management and aerator are sprayed water, and the use that mushroom combines aerator at fine day is sprayed water 1 time, and cloudy day or rainy day can exempt from Except water spray, do not have aerator pond can in conjunction with every day normal management and gather mushroom time carry out moisturizing;
(6) gather: after the edible fungi of cultivation reaches food sanitation standard, can gather, the bacterium bag after gathering does fish meal and returns to fish The pool cultivates.
Further, described floating bed is polygonized structure, is circular hole in the middle of it, and hole internal diameter is 15,18 and 21 centimetres three kinds Specification, regulation and control water quality uses 15 centimetres, and the water surface beautifies or view and admire employing 18 centimetres, and winter, water-surface cultivation used 21 centimetres 's.
Further, described fermentation is 10-20 mesh for work matrix powder is broken to particle mean size, adds water to culture medium prescription aqueous Amount is 64-66wt%, and spice is uniform, and building heap ferments, and when material temperature reaches 60-70 DEG C, starts timing, turns over after 22-26 hour Heap, turning mode is turned up in being;Then every turning in 2-3 days once, turning 3 times, obtains compost altogether;Described pack is will The compost fermented packs;Described sterilizing: the bacterium bag installed is carried out sterilizing, takes autoclaving or normal according to condition Pressure sterilizing;Described be cooled to sterilizing after bacterium bag cool to natural temperature;Described inoculation is that bacterium bag is sent into transfer room or inoculation Inoculation in case;After method is bacterium bag sterilizing cooling, opening bag mouth, strain is put into bag, inoculum concentration is for being equivalent to compost The 5-10% of dry weight.
Compared with prior art, present invention have the advantage that the improvement of the first culture medium of edible fungus, deduct in conventional formulation Wheat bran or Testa oryzae content, utilize bacterium bag to absorb eutrophy composition ammonia nitrogen, organophosphor and the mineral element etc. of pond water quality;Next to that Edible fungus variety selects targetedly, and the high temperature modification kind of Pleurotus ostreatus class is selected in the regulation and control for pond water quality, beautifies for the water surface With view and admire, can use Auricularia, Ganoderma and treasure mushroom kind, for pond comprehensive utilization pursue high yield select Pleurotus ostreatus class low form Kind;3rd floating bed size or the type selected according to different cultivars is the most different, and bacterium bag specification is the most different;4th floating bed all uses Blue guard is edible fungi bag overcover, and blue light, most beneficial for the differentiation of all kinds of fruit body of edible fungi and growth, is built edible for it Good ecological (illumination, temperature, humidity and ventilation condition reach perfect condition) subenvironment of bacteria growing;There is pond cultivation food again Can spray water and reduce artificial mark spraying waterside number with aerator with bacterium, save labour;Utilize floating bed cultivation also can save mushroom house or mushroom shed is built If putting into, making the pond water surface be comprehensively utilized, on the basis of aquaculture, carrying out water surface planting edible mushroom, and may utilize out The bacterium bag of complete mushroom does fish meal and returns to breed in stew, makes aquaculture and mushroom plantation form the efficient of mutually utilization of mutually promoting The ecological circulation agricultural of environmental protection, highlights floating bed cultivation edible fungi and has the features such as high yield potentiation than conventional chambers cultivation.
Detailed description of the invention
The present invention is described in further detail below.
Embodiment 1
The method of a kind of pond water face floating bed cultivation Pleurotus ostreatus, comprises the following steps:
Substrate prepares: selecting formula according to different edible fungus varieties, Pleurotus ostreatus class uses cotton seed hulls 60-80wt%, bagasse 10-15wt%, corn cob 5-10wt%, calcium carbonate 2-5wt%, calcium superphosphate 2-5wt%, sodium selenite 1-2wt%, quick lime 1-2%, its summation is 100%, pH value 8.0;
Substrate fermentation, pack, sterilizing, cooling, inoculation, inoculation is all bacterium Bao Yitou inoculation, and fruiting is the most all one and erects out Mushroom, is different from the conventional two that produces and inoculates, and two accumbency fruiting, this is also that water-surface cultivation mode determines;
Make floating bed: floating bed is polygonized structure, including chassis and the baffle plate of chassis periphery of placement bacterium bag, between baffle plate mutually Connecting, the height of baffle plate can be adjusted according to the size of bacterium bag, chassis is equipped with a net torr and bacterium bag and chassis is isolated Increasing breathability, bacterium bag is directly placed in net torr, has the hole that moisturizes of ellipse, add on the top of floating bed on baffle plate The blue guard of lid, guard half ventilation state makes that sporophore growth is the most lopsided ensures again certain humidity, and blue guard makes son real Body accepts intensity of illumination 200~1000lx, and wavelength is 0.4~0.5 μm;
Bacterium bag and floating bed are placed, that the bacterium bag covering with mycelia is opened inoculation, cleaning surface irregularities and plastic bag is turned down to Bacterium bag surface, bacterium bag bottom plastic bag cuts off 1/5, makes bacterium bag mycelia directly can contact with pond water, and erects in the middle of placement floating bed, Build blue guard, the most sliceable various figures of floating bed above;
Mushroom management and aerator are sprayed water, and the use that mushroom combines aerator at fine day is sprayed water 1 time, and cloudy day or rainy day can exempt spray Water, do not have aerator pond can in conjunction with every day normal management and gather mushroom time carry out moisturizing;
Gather: when cultivation edible fungi reach food sanitation standard after, can gather, the bacterium bag after gathering do fish meal return to fish pond support Grow.
Described floating bed is polygonized structure, is circular hole in the middle of it, and hole internal diameter is 15,18 and 21 centimetres of three kinds of specifications, regulation and control Water quality uses 15 centimetres, and the water surface beautifies or view and admire employing 18 centimetres, and winter, water-surface cultivation used 21 centimetres.
Described fermentation for work matrix powder is broken to particle mean size is 10-20 mesh, add water to culture medium prescription water content be 64-66 Wt%, spice is uniform, builds heap and ferments, and when material temperature reaches 60-70 DEG C, starts timing, turning, turning side after 22-26 hour Formula is turned up in being;Then every turning in 2-3 days once, turning 3 times, obtains compost altogether;Described pack is the training that will ferment Nutriment packs;Described sterilizing: the bacterium bag installed is carried out sterilizing, takes autoclaving or normal-pressure sterilization according to condition;Institute State the bacterium bag after being cooled to sterilizing to cool to natural temperature;Described inoculation is that bacterium bag is sent into inoculation in transfer room or inoculating hood; After method is bacterium bag sterilizing cooling, opening bag mouth, strain is put into bag, inoculum concentration is to be equivalent to the 5-10% of compost dry weight.
Embodiment 2
A kind of pond water edema over the face bed fungus cultivation, Lentinus Edodes or the method for Ganoderma, comprise the following steps:
Substrate prepares: Auricularia, Lentinus Edodes or Ganoderma use cotton seed hulls 50-60wt%, bagasse 15-25wt%, corn cob 10-15wt%, Testa oryzae 5-10wt%, calcium carbonate 2-5wt%, calcium superphosphate 2-5wt%, sodium selenite 2-5wt%, quick lime 1-2%, its summation It is 100%;
Substrate fermentation, pack, sterilizing, cooling, inoculation, inoculation is all bacterium Bao Yitou inoculation, and fruiting is the most all one and erects out Mushroom, is different from the conventional two that produces and inoculates, and two accumbency fruiting, this is also that water-surface cultivation mode determines;
Make floating bed: floating bed is polygonized structure, including chassis and the baffle plate of chassis periphery of placement bacterium bag, between baffle plate mutually Connecting, the height of baffle plate can be adjusted according to the size of bacterium bag, chassis is equipped with a net torr and bacterium bag and chassis is isolated Increasing breathability, bacterium bag is directly placed in net torr, has the hole that moisturizes of ellipse, add on the top of floating bed on baffle plate The blue guard of lid, guard half ventilation state makes that sporophore growth is the most lopsided ensures again certain humidity, and blue guard makes son real Body accepts intensity of illumination 200~1000lx, and wavelength is 0.4~0.5 μm;
Bacterium bag and floating bed are placed, that the bacterium bag covering with mycelia is opened inoculation, cleaning surface irregularities and plastic bag is turned down to Bacterium bag surface, bacterium bag bottom plastic bag cuts off 1/5, makes bacterium bag mycelia directly can contact with pond water, and erects in the middle of placement floating bed, Build blue guard, the most sliceable various figures of floating bed above;
Mushroom management and aerator are sprayed water, and the use that mushroom combines aerator at fine day is sprayed water 1 time, and cloudy day or rainy day can exempt spray Water, do not have aerator pond can in conjunction with every day normal management and gather mushroom time carry out moisturizing;
Gather: when cultivation edible fungi reach food sanitation standard after, can gather, the bacterium bag after gathering do fish meal return to fish pond support Grow.
Described floating bed is polygonized structure, is circular hole in the middle of it, and hole internal diameter is 15,18 and 21 centimetres of three kinds of specifications, regulation and control Water quality uses 15 centimetres, and the water surface beautifies or view and admire employing 18 centimetres, and winter, water-surface cultivation used 21 centimetres.
Described fermentation for work matrix powder is broken to particle mean size is 10-20 mesh, add water to culture medium prescription water content be 64-66 Wt%, spice is uniform, builds heap and ferments, and when material temperature reaches 60-70 DEG C, starts timing, turning, turning side after 22-26 hour Formula is turned up in being;Then every turning in 2-3 days once, turning 3 times, obtains compost altogether;Described pack is the training that will ferment Nutriment packs;Described sterilizing: the bacterium bag installed is carried out sterilizing, takes autoclaving or normal-pressure sterilization according to condition;Institute State the bacterium bag after being cooled to sterilizing to cool to natural temperature;Described inoculation is that bacterium bag is sent into inoculation in transfer room or inoculating hood; After method is bacterium bag sterilizing cooling, opening bag mouth, strain is put into bag, inoculum concentration is to be equivalent to the 5-10% of compost dry weight.
Above-described embodiment, only for technology design and the feature of the explanation present invention, its object is to allow and is familiar with personage's energy of this art Solution present disclosure much of that is also carried out, and can not limit the scope of the invention with this, all real according to spirit of the present invention The equivalence that matter is made changes or modifies, and effect is similar, all should contain within the scope of the present invention.

Claims (3)

1. a method for pond water edema over the face bed culturing edible fungus, comprises the following steps:
(1) substrate prepares: selecting formula according to different edible fungus varieties, Pleurotus ostreatus class uses cotton seed hulls 60-80wt%, bagasse 10-15wt%, corn cob 5-10wt%, calcium carbonate 2-5wt%, calcium superphosphate 2-5wt%, sodium selenite 1-2wt%, Quick lime 1-2%, its summation is 100%, pH value 8.0;Auricularia, Lentinus Edodes or Ganoderma use cotton seed hulls 50-60wt%, Bagasse 15-25wt%, corn cob 10-15wt%, Testa oryzae 5-10wt%, calcium carbonate 2-5wt%, calcium superphosphate 2-5wt%, sodium selenite 2-5wt%, quick lime 1-2%, its summation is 100%;
(2) substrate fermentation, pack, sterilizing, cooling, inoculation, inoculation is all bacterium Bao Yitou inoculation, and fruiting is the most all one Erectting fruiting, be different from the conventional two that produces and inoculate, two accumbency fruiting, this is also that water-surface cultivation mode determines;
(3) make floating bed: floating bed is polygonized structure, including placing the chassis of bacterium bag and the baffle plate of chassis periphery, baffle plate it Between interconnect, the height of baffle plate can be adjusted according to the size of bacterium bag, chassis is equipped with one net torr Bacterium bag and chassis being isolated and increase breathability, bacterium bag is directly placed in net torr, has ellipse on baffle plate Moisturize hole, adds a cover blue guard on the top of floating bed, and guard half ventilation state makes sporophore growth the most abnormal Shape ensures again certain humidity, and blue guard makes sporophore accept intensity of illumination 200~1000lx, and wavelength is 0.4~0.5 μm;
(4) bacterium bag and floating bed are placed, that the bacterium bag covering with mycelia is opened inoculation, clear up surface irregularities and by plastic bag Turning down to bacterium bag surface, bacterium bag bottom plastic bag cuts off 1/5, makes bacterium bag mycelia directly can contact with pond water, and Erect and place in the middle of floating bed, build blue guard, the most sliceable various figures of floating bed above;
(5) mushroom management and aerator are sprayed water, and the use that mushroom combines aerator at fine day is sprayed water 1 time, and cloudy day or rainy day can Exempt water spray, do not have aerator pond can in conjunction with every day normal management and gather mushroom time carry out moisturizing;
(6) gather: after the edible fungi of cultivation reaches food sanitation standard, can gather, the bacterium bag after gathering does fish meal and returns to Breed in stew.
2. the method for pond water edema over the face bed culturing edible fungus as claimed in claim 1, it is characterised in that: described floating bed is polygon Structure, is circular hole in the middle of it, and hole internal diameter is 15,18 and 21 centimetres of three kinds of specifications, and regulation and control water quality employing hole internal diameter is 15 centimetres, the water surface beautifies or views and admires employing hole internal diameter is 18 centimetres, and winter, water-surface cultivation used hole internal diameter to be 21 lis Rice.
3. the method for pond water edema over the face bed culturing edible fungus as claimed in claim 1, it is characterised in that: described fermentation is by work base It is 10-20 mesh that matter is crushed to particle mean size, and adding water to culture medium prescription water content is 64-66wt%, and spice is uniform, builds Heap ferments, and when material temperature reaches 60-70 DEG C, starts timing, and turning after 22-26 hour, turning mode is turned up in being;So After every turning in 2-3 days once, altogether turning 3 times, obtains compost;Described pack is to be packed by the compost fermented; Described sterilizing: the bacterium bag installed is carried out sterilizing, takes autoclaving or normal-pressure sterilization according to condition;Described be cooled to by Bacterium bag after sterilizing cools to natural temperature;Described inoculation is that bacterium bag is sent into inoculation in transfer room or inoculating hood;Method is After bacterium bag sterilizing cooling, opening bag mouth, strain is put into bag, inoculum concentration is to be equivalent to the 5-10% of compost dry weight.
CN201610247014.2A 2016-04-20 2016-04-20 Method for cultivating edible mushrooms with floating bed on water surface of pond Pending CN105900683A (en)

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Inventor after: Wu Damin

Inventor after: Zou Xinjian

Inventor after: Zeng Liugen

Inventor after: Zhang Dongping

Inventor after: Liu Changlin

Inventor after: Hong Xiangjiao

Inventor after: Yao Yi

Inventor after: Liu Hui

Inventor after: Lan Lan

Inventor after: Xu Liangqing

Inventor before: Wu Damin

Inventor before: Zeng Liugen

Inventor before: Zhang Dongping

Inventor before: Liu Changlin

Inventor before: Hong Xiangjiao

Inventor before: Yao Yi

Inventor before: Liu Hui

Inventor before: Lan Lan

Inventor before: Xu Qingliang

RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20160831