CN105993587A - Method for studying influence of pleurotus ferulae mushroom fungus liquid on desert plant ferula seedling growth - Google Patents
Method for studying influence of pleurotus ferulae mushroom fungus liquid on desert plant ferula seedling growth Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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Abstract
The invention mainly relates to the technical field of plant cultivation, and specifically relates to a method for studying the influence of pleurotus ferulae mushroom fungus liquid on desert plant ferula seedling growth. According to the method, a pleurotus ferulae mushroom fungus liquid is prepared; ditches are provided at the root parts of ferula seedlings, and the pleurotus ferulae mushroom fungus liquid is used for irrigation; the influence of the pleurotus ferulae mushroom fungus liquid on ferula seedling growth is studied in three aspects; a control group is set; on the first aspect, the influence of the pleurotus ferulae mushroom fungus liquid on the dry biomass, fresh biomass and root growth condition of the ferula seedlings is studied; on the second aspect, the influence of the pleurotus ferulae mushroom fungus liquid on the growth and stress resistance of the ferula seedlings is studied; and on the third aspect, the influence of the pleurotus ferulae mushroom fungus liquid on ferula seedling root nutrient absorption and accumulation is studied. The result of the influence of the pleurotus ferulae mushroom fungus liquid on the desert plant ferula seedling growth is used in ferula plant artificial cultivation and resource protection.
Description
Technical field
The present invention principally falls into field of plant growing technology, is specifically related to study Pleurotus ferulae Lanzi fungus bacterium
The method that ermophyte Resina Ferulae growth of seedling is affected by liquid.
Background technology
Resina Ferulae plant is under the jurisdiction of Umbelliferae (Apiaceae Lindley or Umbelliferae Jussieu)
Celery subfamily (Apioideae Drude) Radix Peucedani race (Peucedaneae Drude) Resina Ferulae subtribe
(Ferulinae Drude) Ferula (Ferula Linne), is perennial once result or repeatedly
The class in early spring of result is died young draft, and the whole world has more than 150 to plant, and is mainly distributed in south of europe ground
Haiti district, the north, Africa, Central Asia and the west and south, Asia.Ferula sinkiangensisK.M.Shen (Ferula
Sinkiangensis K.M.Shen) it is grown on the cement slight slope of desert and band gravel in front of the mountains, only
Be distributed in Yili of Xinjiang (visiing the stone pier) narrow and small area, as China's Resina Ferulae former plant it
One was gone through version " Pharmacopoea Chinensis " after 1977 records, and its resin produced is commonly called as Resina Ferulae (glue) and replaces
For import Resina Ferulae, allow China thoroughly finish the history of import, be China's Chinese medicine material, taste
Bitter, pungent, warm in nature, there are detumescence of regulating the flow of vital energy, tired the eliminating the phlegm and effect of excitor nerve that disappear of invigorating blood circulation, tie up me
You doctor is normal in anthelmintic, treatment vitiligo, gastropathy, arthritis etc., has high medical value.
In recent years, because finding Resina Ferulae has phytoestrogenic activity composition and cancer-resisting substance and extremely people
Pay close attention to.But, owing to Resina Ferulae plant resources indiscriminate mining and serious waste, uncontrolled herding and excessively opening
Reclaim wasteland ground and Urbanization Process In China " resource cures ", cause Vulnerable Ecological serious unbalance,
Habitat is forced transition, and distribution area extremely atrophy, important species are endangered, two packing spaces
Property meet with reduce even lose condition.Therefore, for protection Resina Ferulae resource set up effective manually
Technical system is the most urgent.
Pleurotus ferulae Lanzi (Pleurotus ferulae Lenzi), has another name called Pleurotus ferulae Lanzi, asafoetida mushroom, because of open country
The raw parasitic or saprophytic rhizome portion loose Chinese medicine Resina Ferulae plant, and obtain popular name Pleurotus ferulae Lanzi.Resina Ferulae
Mushroom returns is subordinate in Basidiomycotina (Basidiomycotina) Hymenomycetes (Hymenomycetes)
Agaricales (Agaricales) Tricholomataceae (Tricholomataceae) pleurotus (Pleurotus),
Compared with other kinds of pleurotus, the quality (including cap and stem) of Pleurotus ferulae Lanzi is tenderer, fragrant
Taste is richer, is that the cooking of this genus is worth an optimal kind, and the Pleurotus ferulae Lanzi wild in China is detected in
Xinjiang.China's Traditional Chinese Medicine thinks that Pleurotus ferulae Lanzi has the efficacy of drugs identical with Chinese medicine Resina Ferulae.
In nature, nearly all plant all becomes the residential location of microorganism, especially plant
Root, so two or more biology relation of combining closely include that pseudosymphile, commensalism are common
Three main Types such as life and Mutualism, wherein pseudosymphile and Mutualism Bu Shiliangge circle
Limit is individually present action mode clearly, but constitutes a continuous symbiosis spectrum, along with environmental condition,
Evolutionary process and convert, they to host's existence, reproductive capacity, Biomass, rot decompose, plant
The adaptive characters such as group motion state, defence and the impact of ecosystem function own profound.Study table recently
Bright, synbiosis form is to interdepend and more more complicated than previously imagined, is not the most letter
Singly being fixed as a type, Mutualism-pseudosymphile becomes continuum, and these are altogether
Raw body has positive interaction and negative interaction simultaneously, and symbiosis two components adaptive to host are raw
Deposit and have the biggest impact of difference with reproductive capacity.I.e. come from parasitism when the action mode of Mutualism
During symbiosis, needing to pay big cost, reproductive capacity is transformed into existence from resource is distributed by host,
This is to understand Mutualism from another angle, to existence positive interaction with to reproductive capacity negative interaction
It it is the key of Mutualism foundation.It addition, be the adaptive raising of host, host again can be from money
In the distribution of source, existence is changed back to reproductive capacity.So far, to Resina Ferulae plant and Pleurotus ferulae Lanzi relation
Research be concentrated mainly on interpolation Resina Ferulae plant component after to pleuratus ferulae comatus mycelium growth, sporophore
Grow and research in terms of quality and effect, find that it can improve Pleurotus ferulae Lanzi cultivating rate, promote Ah
Wei mushroom growth promoter and the formation of sporophore.But, Pleurotus ferulae Lanzi fungus bacterium solution is to ermophyte
The impact of Resina Ferulae growth of seedling and effect, have no that correlational study is reported up to now.
Summary of the invention
For the problems referred to above, the present invention provides a kind of research Pleurotus ferulae Lanzi fungus bacterium solution to ermophyte
The method of Resina Ferulae growth of seedling impact, described method passes through three aspect research Pleurotus ferulae Lanzi bacterium solution pair
The impact of Resina Ferulae growth of seedling, three aspects are respectively research Pleurotus ferulae Lanzi bacterium solution and do Resina Ferulae seedling
Biomass, fresh Biomass and the impact of root growth situation, to Resina Ferulae growth of seedling and resistance
Affecting and on Resina Ferulae seedling root Nutrient Absorption and the impact of accumulation, the method for the invention is
The research growth promoter of Resina Ferulae plant, cultivation technique and Proterozoic protection provide technical support.
The present invention is achieved by the following technical solutions:
The method that ermophyte Resina Ferulae growth of seedling is affected by research Pleurotus ferulae Lanzi fungus bacterium solution, described
First method prepares Pleurotus ferulae Lanzi bacterium solution, to Resina Ferulae seedling in root trench digging pouring Pleurotus ferulae Lanzi bacterium solution,
Matched group is set；Then three aspect research Pleurotus ferulae Lanzi bacterium solution shadow to Resina Ferulae growth of seedling is passed through
Ring, first aspect, with Resina Ferulae seedling different parts dry matter weight, fresh weight and root length degree,
The a diameter of index of root, research Pleurotus ferulae Lanzi bacterium solution is to Resina Ferulae seedling dry biomass, fresh Biomass and root
The impact of upgrowth situation；Second aspect, analyzes and Resina Ferulae growth of seedling and degeneration-resistant relevant physiology
Index, research Pleurotus ferulae Lanzi bacterium solution is on Resina Ferulae growth of seedling and the impact of resistance；The third aspect,
With seedling root nutrition accumulation as index, research Pleurotus ferulae Lanzi bacterium solution is to Resina Ferulae seedling root nutrient
Absorb and the impact of accumulation.
Further, the research method of described first aspect is particularly as follows: with 1 year raw seedling for grinding
Study carefully object, to Resina Ferulae seedling root pouring Pleurotus ferulae Lanzi bacterium solution after 0d, 15d, 30d,
45d and 60d is separately sampled, to gather Resina Ferulae seedling carry out pretreatment after, measure Ah
Wei Youmiao overground part and root dry matter weight, fresh weight and root length degree and root diameter, with research
Pleurotus ferulae Lanzi bacterium solution is on Resina Ferulae seedling dry biomass, fresh Biomass and the impact of root growth situation.
Further, the research method of described second aspect is particularly as follows: with 1 year raw seedling for grinding
Study carefully object, to Resina Ferulae seedling root pouring Pleurotus ferulae Lanzi bacterium solution after 0d, 15d, 30d,
45d and 60d is separately sampled, to gather Resina Ferulae seedling carry out pretreatment after, measure Ah
Wei Youmiao overground part and root and Resina Ferulae growth of seedling and degeneration-resistant relevant physical signs；Described with
Resina Ferulae growth of seedling and degeneration-resistant relevant physical signs include superoxide dismutase activity, peroxide
Compound enzymatic activity, mda content, free proline content, content of soluble protein, can
Dissolubility sugar content；The mensuration of superoxide dismutase activity uses nitroblue tetrazolium photochemical reduction method,
The mensuration of peroxidase activity uses guaiacol method, and the mensuration of free proline content uses
Sulfosalisylic acid colorimetric method, the mensuration of mda content uses thiobarbituric acid reaction method, can
The mensuration of soluble proteins content uses Coomassie brilliant G-250 staining, and soluble sugar contains
The mensuration of amount uses anthrone ethyl acetate method；Result is 3 meansigma methodss repeated.
Further, the described third aspect research method particularly as follows: with 1 year raw seedling and
2 years raw seedling are object of study, after Resina Ferulae seedling root the last time pouring Pleurotus ferulae Lanzi bacterium solution
0d, 15d, 30d, 45d and 60d separately sampled, to the Resina Ferulae gathered
After seedling carries out pretreatment, measure Resina Ferulae seedling root nutrition accumulation；Described Resina Ferulae seedling root
Portion's nutrition accumulation includes that N accumulation, N stage accumulation, N stage accumulation account for total amount
Ratio, P2O5Accumulation, P2O5Stage accumulation, P2O5Stage accumulation account for sum-rate,
K2O accumulation, K2O stage accumulation, K2O stage accumulation accounts for sum-rate.
Further, the Resina Ferulae seedling gathered is carried out pretreatment particularly as follows: divided by Resina Ferulae seedling
For overground part and root, at 120 DEG C of 30min that complete, then dry to constant weight in 80 DEG C,
Claim dry weight, grinding, put into valve bag preserves to be measured.
Further, during sampling, randomly select grow fine and grow basically identical 10～
20 strain Resina Ferulae seedling plants.
Further, the preparation method of described Pleurotus ferulae Lanzi bacterium solution particularly as follows: under aseptic condition,
Cultivate Pleurotus ferulae Lanzi bacterial strain in PDA culture medium and cover with culture dish to Pleurotus ferulae Lanzi, culture dish will be covered with
Pleurotus ferulae Lanzi bacterial strain card punch take a diameter of 0.6cm truffle, by described truffle access equipped with send out
In the triangular flask of ferment culture medium, it is placed in 24 DEG C, in the shaking table of 120r/min, cultivates 30d,
Bacterium solution is filtered, collects supernatant, i.e. obtain described Pleurotus ferulae Lanzi bacterium solution: described fermentation medium
In the concentration of each component be: Rhizoma Solani tuber osi 25g/L, glucose 20g/L, peptone 3g/L, phosphorus
Acid dihydride potassium 2g/L, magnesium sulfate 1g/L.
Further, to Resina Ferulae seedling in root trench digging pouring Pleurotus ferulae Lanzi bacterium solution particularly as follows: Resina Ferulae
The pouring of mushroom liquid uses ditch spread, and at root trench digging, the Resina Ferulae seedling of drilling is watered Resina Ferulae mushroom
Liquid, the irrigation amount of Pleurotus ferulae Lanzi bacterium solution controls at 0.5L/ rice row long, and matched group does not water Pleurotus ferulae Lanzi
Bacterium solution.
The Advantageous Effects of the present invention:
(1) present invention provides a kind of research Pleurotus ferulae Lanzi fungus bacterium solution to ermophyte Resina Ferulae seedling
The method of growth effect, described method is by research Pleurotus ferulae Lanzi bacterium solution biology dry to Resina Ferulae seedling
Amount, fresh Biomass and the impact of root growth situation, disclose during Resina Ferulae growth of seedling weight and
The change of size and conversion thereof, thus lay the foundation for the research of ermophyte Resina Ferulae growth promoter.
(2) the method for the invention and is resisted Resina Ferulae growth of seedling by research Pleurotus ferulae Lanzi bacterium solution
The impact of inverse property, provides a kind of method, from physiology for inquiring into the degeneration-resistant mechanism of ermophyte Resina Ferulae
Reason is provided for Ferula sinkiangensisK.M.Shen environmental suitability mechanism with carrying out Proterozoic protection on ecological point
Opinion foundation.
(3) the method for the invention is by studying Pleurotus ferulae Lanzi bacterium solution to Resina Ferulae seedling root nutrient
Absorb and the impact of accumulation, disclose nutrient accumulation and distribution spy in Resina Ferulae growth promoter forming process
Levy, provide technical support for Resina Ferulae plant artificial culture.
Accompanying drawing explanation
Fig. 1 Pleurotus ferulae Lanzi bacterium solution is fresh to Resina Ferulae seedling aerial parts (a figure) and root (b figure)
The impact of weight；
Fig. 2 Pleurotus ferulae Lanzi bacterium solution is to Resina Ferulae seedling root length degree (a figure) and root diameter (b figure)
Impact；
Fig. 3 Pleurotus ferulae Lanzi bacterium solution is to Resina Ferulae seedling overground part (a figure) and root (b figure) dry
The impact of matter weight；
Fig. 4 Pleurotus ferulae Lanzi bacterium solution is to Resina Ferulae seedling aerial parts (a figure) and root (b figure) SOD
The impact of activity；
Fig. 5 Pleurotus ferulae Lanzi bacterium solution is to Resina Ferulae seedling aerial parts (a figure) and root (b figure) POD
The impact of activity；
Fig. 6 Pleurotus ferulae Lanzi bacterium solution is to Resina Ferulae seedling aerial parts (a figure) and root (b figure) MDA
The impact of content；
Resina Ferulae seedling aerial parts (a figure) and root (b figure) are swum by Fig. 7 Pleurotus ferulae Lanzi bacterium solution
Impact from proline content；
Fig. 8 Pleurotus ferulae Lanzi bacterium solution can to Resina Ferulae seedling aerial parts (a figure) and root (b figure)
The impact of dissolubility protein content；
Fig. 9 Pleurotus ferulae Lanzi bacterium solution can to Resina Ferulae seedling aerial parts (a figure) and root (b figure)
The impact of dissolubility sugar content；
To Resina Ferulae seedling root different times, (a figure is 1a seedling to Figure 10 Pleurotus ferulae Lanzi bacterium solution, b
Figure is 2a seedling) N accumulation dynamic change impact；
To Resina Ferulae seedling root different times, (a figure is 1a seedling to Figure 11 Pleurotus ferulae Lanzi bacterium solution, b
Figure is 2a seedling) P2O5The impact of accumulation dynamic change；
Figure 12 Pleurotus ferulae Lanzi bacterium solution is to 1a Resina Ferulae seedling root K2The impact of O accumulation dynamic change；
Figure 13 Pleurotus ferulae Lanzi bacterium solution is to 2a Resina Ferulae seedling root K2The impact of O accumulation dynamic change.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearer, below in conjunction with
Drawings and Examples, are explained in further detail the present invention.Should be appreciated that this place is retouched
The specific embodiment stated is used only for explaining the present invention, is not intended to limit the present invention.
On the contrary, the present invention contains any spirit and scope in the present invention being defined by the claims
On replacement, amendment, equivalent method and the scheme made.Further, in order to make the public to this
Bright having a better understanding, during the details to the present invention describes below, detailed to describe some special
Fixed detail section.The description not having these detail sections for a person skilled in the art also may be used
To understand the present invention completely.
Embodiment 1
The method that ermophyte Resina Ferulae growth of seedling is affected by research Pleurotus ferulae Lanzi fungus bacterium solution, described
First method prepares Pleurotus ferulae Lanzi bacterium solution, to Resina Ferulae seedling in root trench digging pouring Pleurotus ferulae Lanzi bacterium solution,
Matched group is set；Then three aspect research Pleurotus ferulae Lanzi bacterium solution shadow to Resina Ferulae growth of seedling is passed through
Ring, first aspect, with Resina Ferulae seedling different parts dry matter weight, fresh weight and root length degree,
The a diameter of index of root, research Pleurotus ferulae Lanzi bacterium solution is to Resina Ferulae seedling dry biomass, fresh Biomass and root
The impact of upgrowth situation；Second aspect, analyzes and Resina Ferulae growth of seedling and degeneration-resistant relevant physiology
Index, research Pleurotus ferulae Lanzi bacterium solution is on Resina Ferulae growth of seedling and the impact of resistance；The third aspect,
With seedling root nutrition accumulation as index, research Pleurotus ferulae Lanzi bacterium solution is to Resina Ferulae seedling root nutrient
Absorb and the impact of accumulation.
First aspect research Pleurotus ferulae Lanzi bacterium solution is to Resina Ferulae seedling dry biomass, fresh Biomass and root growth shape
The concrete operations that affect of condition are:
1 materials and methods
1.1 sample plot overviews
Experimental field it is positioned at Xinjiang academy of agricultural sciences Manas' Experimental Base, east longitude 85 ° 34 ' to 86 ° 43 ',
North latitude 43 ° 28 ' to 45 ° 38 '.Experimental plot soil water-soluble nitrogen 63.5mg/kg, rapid available phosphorus
14.5mg/kg, available potassium 309mg/kg.
1.2 material to be tested
For examination Pleurotus ferulae Lanzi bacterial strain: 2 Pleurotus ferulae Lanzi bacterial strain W1, W5 gather respectively from Tacheng
Area Yumin County, Qinghe County, Altay Prefecture.
For examination Resina Ferulae plant seed: Ferula sinkiangensisK.M.Shen seed was collected from Yi Li in 2013 and 2014
Stone pier mountain area is visitd in Kazak autonomous prefecture Yining County Keshen township.
Prepared by 1.3 fermentation liquids
The preparation method of described Pleurotus ferulae Lanzi bacterium solution is particularly as follows: under aseptic condition, cultivate at PDA
Cultivate Pleurotus ferulae Lanzi bacterial strain on base and cover with culture dish to Pleurotus ferulae Lanzi, the Resina Ferulae mushroom of culture dish will be covered with
Strain card punch takes a diameter of 0.6cm truffle, accesses described truffle equipped with fermentation medium
(Rhizoma Solani tuber osi 25g/L, glucose 20g/L, peptone 3g/L, potassium dihydrogen phosphate 2g/L, sulfur
Acid magnesium 1g/L) triangular flask in, be placed in 24 DEG C, in the shaking table of 120r/min, cultivate 30d,
Collect standby.
1.4 test method
Sowing: raw seedling was sowed in October, 2014 in 1 year.
Bacterium solution pouring uses ditch spread: on May 1st, 2015 to the Resina Ferulae seedling of drilling at root
Trench digging pouring Pleurotus ferulae Lanzi bacterium solution；Pleurotus ferulae Lanzi fermentation liquid 3L (the most every 6 meters of 3L bacterium solution).Do not water
Bacterium solution is comparison (CK).
Field management: remove weeds in time.
Sampling: grow 0d (May 1), 15d (May 15), 30d (5 respectively after watering bacterium
Months 30 days), 45d (June 15), five samplings in periods of 60d (July 1).When each
Phase, randomly select and grow fine and grow basically identical 10～20 strain plant, take back after sampling
Laboratory.
Sample determination and process: wash away silt, blot surface moisture, measure overground part fresh weight,
Highly, root fresh weight, length, thickness, then put in culture dish, after 120 DEG C of 30min that complete
Dry to constant weight for 80 DEG C, claim dry weight, grinding, put into valve bag preserves to be measured.
1.5 data analysis
With Excel2010, data are arranged.
2 results and analysis
The impact on Resina Ferulae different parts dynamic changes of fresh weight of 2.1 bacterium solution
As seen from Figure 1, along with the passage of growth time, Resina Ferulae seedling overground part and root
Fresh weight accumulation all present first rise after downward trend, and pouring bacterium solution can promote Resina Ferulae seedling
Accumulation to fresh weight, all shows as W5 > W1 > CK, Resina Ferulae seedling overground part 15d after watering bacterium
Reach peak value, respectively 0.25mg/ strain, 0.23mg/ strain, 0.19mg/ strain, the most relatively compare
High by 31.58%, 20.05%；Root 45d after watering bacterium reaches peak value, respectively 0.64mg/
Strain, 0.63mg/ strain, 0.57mg/ strain, the most relatively compare high by 12.28%, 10.53%.Show,
Bacterium solution has facilitation, and the work to overground part to Resina Ferulae plant above ground portion, the accumulation of root fresh weight
With > root, the facilitation of bacterium solution W5 is more than W1.
The impact on Resina Ferulae seedling root growth of 2.2 bacterium solution
As seen from Figure 2, with the passage of Resina Ferulae growth time, the root length of Resina Ferulae seedling root,
Root the most all presents first ascendant trend, and pouring Pleurotus ferulae Lanzi bacterium solution can promote the growth of Ah's root,
All show as W5 > W1 > CK, after watering bacterium 60d, root length be respectively 12.50cm, 12.00cm,
11.28cm, the most relatively comparison increase by 10.82%, 3.38%；Root slightly respectively 0.501cm,
0.496cm, 0.463cm, the most relatively comparison increase by 8.21%, 7.13%.Show bacterium solution to Ah
The root length of Wei Youmiao root, root slightly have facilitation, and the facilitation of bacterium solution W5 is more than
W1。
The impact that Resina Ferulae different parts dry-matter accumulation is dynamically changed by 2.3 bacterium solution
As seen from Figure 3, along with the passage of growth time, Resina Ferulae seedling overground part and root
Dry-matter accumulation all present first rise after downward trend, and pouring Pleurotus ferulae Lanzi bacterium solution can promote
The accumulation to dry of the Resina Ferulae seedling, all shows as W5 > W1 > CK, Resina Ferulae seedling overground part
Peaking during 30d after watering bacterium, respectively 0.050mg/ strain, 0.047mg/ strain, 0.039mg/
Strain, the most relatively compare high by 28.21%, 20.51%；Root 45d after watering bacterium reaches peak value,
Be respectively 0.186mg/ strain, 0.175mg/ strain, 0.164mg/ strain, the most relatively compare high by 13.41%,
6.71%.Showing, bacterium solution has facilitation to Resina Ferulae seedling overground part, root dry-matter accumulation,
The bacterium solution effect to overground part > root, the facilitation of bacterium solution W5 is more than W1.
As can be seen from Table 1, Dry Matter rule has bigger change between Resina Ferulae seedling growing period
Change.Along with the passage of Resina Ferulae growth time, Resina Ferulae total biomass reduces trend afterwards in first raising；
Before just having started i.e. to water bacterium solution, dry be mainly assigned to overground part (can account for Herb 57～
58%), overground part is organic centre before this.Dry is mainly used in the growth of overground part, ground
The ratio that top dry accounts for Herb dry is maximum, but enters May, and leaf photosynthesis produces
Nutrient substance transport downwards, for the growth of root, root dry substantially increases, and makes on the ground
Portion accounts for the ratio of Herb and gradually decreases.The apportionment ratio of dry matter weight of aerial parts accumulation presents decline and becomes
Gesture, after watering bacterium, basic expressions is CK > W1 > W5, and when watering 15d after bacterium, apportionment ratio reduces
The most obvious, relatively water bacterium 0d reduce respectively 29.61% (CK), 29.92% (W1), 30.54%
(W5)；And root Biomass accounting is in rising trend, root apportionment ratio also presents ascendant trend,
Watering bacterium 15d is that root dry-matter accumulation apportionment ratio increase is the most obvious, and relatively watering bacterium 0d increases respectively
29.71% (CK), 30.28% (W1), 30.85% (W5).
The impact on Resina Ferulae seedling different parts dry matter accumulation and allocation characteristic of table 1 bacterium solution
Second aspect research Pleurotus ferulae Lanzi bacterium solution is to Resina Ferulae seedling dry biomass, fresh Biomass and root growth shape
The concrete operations that affect of condition are:
1 materials and methods
1.1 sample plot overviews: with embodiment 1.
1.2 materials to be tested: for examination Pleurotus ferulae Lanzi bacterial strain, for examination Resina Ferulae plant seed, fermentation liquid system
Standby with embodiment 1.
1.3 test methods:
Sowing, bacterium solution pouring, field management, sampling: with embodiment 1.
Physiological index determining:
Superoxide dismutase (SOD) determination of activity nitroblue tetrazolium (NBT) photochemical reduction
Method；Peroxidase (POD) determination of activity guaiacol method；Proline (Pro)
Assay uses sulfosalisylic acid colorimetric method；Malonaldehyde (MDA) assay uses sulfur generation
Barbiturates (TBA) method；Content of soluble protein measures and uses Coomassie brilliant G-250
Staining；Soluble sugar uses anthrone ethyl acetate method.
1.4 data analysiss:
With Excel2010, data are arranged, with SPSS19.0, data are analyzed.
2 results and analysis
The comparison of 2.1 protective enzyme SOD, POD activity
2.1.1 the bacterium solution impact on Resina Ferulae plant SOD activity
Result of study shows, along with the growth of Resina Ferulae, the SOD of Resina Ferulae seedling different parts lives
Property present first rise after downward trend (such as Fig. 4).Can promote after watering bacterium solution Resina Ferulae overground part,
The increase of root SOD content, shows as W1 > W5 > CK.Resina Ferulae overground part is after watering bacterium
15d reaches peak value and is respectively 297.04U/g FW, 286.75U/g FW, 267.39U/g FW,
Amplification is 11.09%, 7.24%；And root position 30d after watering bacterium reaches peak value, it is respectively
185.72U/g FW, 182.82U/g FW, 156.99U/g FW, amplification is 18.30%,
16.45%.Variance analysis shows, between overground part, root W1 and W5 process, difference is not notable,
But in significant difference all and between CK.All in all, bacterial strain W1, W5 bacterium solution is to root
Effect more than overground part.
2.1.2 the bacterium solution impact on Resina Ferulae plant POD activity
Result of study shows, along with the growth of Resina Ferulae, the POD activity of Resina Ferulae overground part presents
Downward trend after first rising, seedling root position then presents to fall before and is rising downward trend again
(such as Fig. 5).Pleurotus ferulae Lanzi bacterium solution can promote Resina Ferulae overground part, root POD content after watering
Increase, show as W1 W5 > CK.Resina Ferulae overground part 15d after watering bacterium has just reached peak value,
Being respectively 3.80U, 3.61U, 3.22U, amplification is 18.01%, 12.11%, and respectively processes
Between diversity notable；Root 30d after watering bacterium has reached maximum, respectively 5.52U, 5.44
U, 4.89U, amplification is 12.88%, 11.25%, and between W1 and W5 process, difference does not shows
Write, but in significant difference all and between CK.All in all, bacterial strain W1, W5 bacterium solution is over the ground
The effect on top is more than root.
The impact on Resina Ferulae plant MDA content of 2.2 bacterium solution
Result of study shows, along with the growth of Resina Ferulae, the MDA content of Resina Ferulae overground part presents
Downward trend (such as Fig. 6) after first rising.After Pleurotus ferulae Lanzi bacterium solution is watered, overground part, root MDA
Reactions vary causes.Resina Ferulae overground part 15d after watering bacterium has reached peak value, shows as
W5 > W1 > CK, respectively 9.73 μm ol/g, 9.14 μm ol/g, 8.41 μm ol/g, amplification
It is 15.70%, 8.68%, and between each process, diversity is notable；Resina Ferulae plant root is then watering
After bacterium, 30d reaches peak value, shows as CK > W1 > W5, respectively 2.05 μm ol/g, 1.86
μm ol/g, 1.74 μm ol/g, it is poor that relatively CK reduces between 9.27%, 15.12%, and each process
The opposite sex is notable.The most apparently, bacterial strain W5, W1 bacterium solution is to Resina Ferulae plant above ground position cell
Membrane damage is heavier, slows down root cell membrane damage, causes the resistance improving root.
The impact on Resina Ferulae plant free proline content of 2.3 bacterium solution
Result of study shows, along with the growth of Resina Ferulae, the Proline of each organ of Resina Ferulae seedling
All present downward trend (such as Fig. 7) after first rising.Pleurotus ferulae Lanzi bacterium solution all can promote after watering Ah
Wei overground part, the increase of root free proline content, all show as W1 > W5 > CK.Ground
Top after watering bacterium 30d be respectively 322.75 μ g/g FW, 290.46 μ g/g FW, 261.35
μ g/g FW, the amplification of W1, W5 is respectively between 23.49%, 11.14%, and each process
All in significant difference；Root 30d after watering bacterium be respectively 384.69 μ g/g FW, 329.10
μ g/g FW, 266.16 μ g/g FW, the amplification of W1, W5 is respectively 44.53%, 23.63%,
And all in significant difference between respectively processing.The most apparently, bacterial strain W1, W5 bacterium solution is to root
Effect more than overground part.
The impact on Resina Ferulae plant soluble protein content of 2.4 bacterium solution
Result of study shows, along with the growth of Resina Ferulae, the solubility egg of Resina Ferulae seedling Different Organs
Bai Hanliang presents downward trend (such as Fig. 8) after first rising.Pleurotus ferulae Lanzi bacterium solution all can promote each after watering
The increase of position soluble protein content, shows as W5 > W1 > CK, and after watering bacterium 30d
Soluble protein content peaking.Overground part be respectively 4.81mg/g FW, 4.73mg/g FW,
4.33mg/g FW, W5, W1 amplification is respectively 11.09%, 9.24%, and bacterium solution W1,
Between W5 processes, difference is not notable, all and presents significant difference between CK.Root of Resina Ferulae portion is respectively
For 2.18mg/g FW, 2.17mg/g FW, 2.03mg/g FW, W5, W1 amplification is respectively
It is 7.39%, 6.90%, and presents significant difference between each process.Overall the most apparently, bacterial strain W1,
W5 bacterium solution is more than root to the effect of overground part.
The impact on Resina Ferulae plant soluble sugar content of 2.5 bacterium solution
Result of study shows, along with the growth of Resina Ferulae, the soluble sugar of each organ of Resina Ferulae seedling in
Downward trend after the most first rising, all reached maximum (such as Fig. 9) June 1.Water bacterium solution
After, bacterium solution suppression Resina Ferulae overground part, the increase of root soluble sugar content.Resina Ferulae overground part table
It is now CK > W5 > W1, respectively 16.45mg/g, 15.60mg/g, 15.14mg/g, W5,
It is not notable that W1 relatively CK reduces difference between 5.17%, 7.69%, and bacterium solution W1, W5 respectively,
All and present significant difference between CK；Resina Ferulae root is CK > W1 > W5, is respectively
54.17mg/g, 47.63mg/g, 45.09mg/g, W1, W5 relatively CK reduces by 12.07% respectively,
Significant difference is presented between 16.76%, and each process.The most apparently, bacterial strain W1, W5 bacterium
Liquid all makes the soluble sugar content of seedling overground part and root reduce.
Third aspect research Pleurotus ferulae Lanzi bacterium solution is to Resina Ferulae seedling root Nutrient Absorption and the shadow of accumulation
Ringing concrete operations is:
1 materials and methods
1.1 sample plot overviews: with embodiment 1.
1.2 materials to be tested: for examination Pleurotus ferulae Lanzi bacterial strain, for examination Resina Ferulae plant seed, fermentation liquid system
Standby with embodiment 1.
1.3 test method
Sowing: 1a seedling was sowed in October, 2013, and 2a seedling was broadcast in October, 2014
Kind；
Bacterium solution is watered: 1a seedling carried out root bacterium solution furrow irrigation, 2a children on May 1st, 2015
Seedling carries out root bacterium solution furrow irrigation respectively on May 1st, 2014, on May 1st, 2015.Often
6 meters of row length water 3L bacterium solution.Do not water bacterium solution for comparison (CK).
Field management: remove weeds in time.
Sampling: respectively after the last time waters bacterium 0d (May 1), 15d (May 15),
30d (May 30), 45d (June 15), five growth periods of 60d (July 1),
Choose and grow fine and grow 10 consistent strain plant, take back laboratory after sampling, cut off ground
Upper part, complete at 120 DEG C 30min by its root, is then dried in 80 DEG C of baking ovens
Constant weight, weighs, grinds, put into valve bag preserves to be measured.
Nutrient measures: sample H2SO4-H2O2Disappearing and boil, nitrogen analysis uses Nai Shi colorimetric
Method；Phosphorus detection uses vanadium molybdenum Huang colorimetry；Potassium content measures and uses flame spectrometry.
N(P2O5、K2O) accumulation (g/plant)=dry matter weight (kg/plant) × full nitrogen
Content (g/kg)
N(P2O5、K2O) nitrogen accumulation of stage accumulation (g/plant)=current generation
Amount-nitrogen accumulation amount for the first period
N(P2O5、K2O) sum-rate (%)=N (P is accounted for2O5、K2O) stage accumulation
Amount/N (P2O5、K2O) maximum accumulation
1.4Logistic equation simulation
With Logistic equation model Resina Ferulae root nutrient accumulation Changing Pattern, Logistic equation
For: Y=K/ (1+ (e(a+bt))).Y is Resina Ferulae root N (P2O5、K2O) accumulation, t is
Resina Ferulae emerge after natural law, a, b, K are undetermined coefficient, t0During (d)=-a/b, t0It is expressed as
N(P2O5、K2O) moment that accumulation rate is maximum, Vm (mg/d)=-bk/4, Vm table
It is shown as N (P2O5、K2O) accumulation maximum rate；Δ t (d)=t2～t1, Δ t is that " time is special
Value indicative ", represent the time length of crop vigorous period (dry-matter accumulation Rapid Accumulation phase),
t1The time that the expression Rapid Accumulation phase starts, t2The time that the expression Rapid Accumulation phase terminates；GT
(mg/plant)=-bK/4 × Δ t, wherein GT is at t1～t2" growth characteristics value " in time,
Represent that the accumulation of dry or nutrient has reached the 65.8% of maximum accumulation.
1.5 data analysiss:
Using Excel2010, DPS7.05 to be analyzed, variance analysis uses LSD method.
2 results and analysis
2.1 bacterium solution on 1, the impact of 2a Resina Ferulae seedling root N accumulation
2.1.1 bacterium solution on 1, the impact of 2a Resina Ferulae root N accumulation dynamic
After bacterium solution pouring, 1, the Resina Ferulae plant root nitrogen accumulation of 2a is with the prolongation of growth time
All in downward trend (Figure 10) after first rising, water rear 45d peaking, show as
W5>W1>CK.Wherein, 1a nitrogen accumulation amount the most relatively compare add 54.10% (W5),
42.58% (W1), and between W1, W5, CK all in significant difference (P ＜ 0.05).2a nitrogen
Element accumulation the most relatively compares and adds 26.71% (W5), 21.21% (W1), bacterium solution W1,
A significant difference is not had between W5, but all with CK all in significant difference (P ＜ 0.05).Show
Bacterium solution has facilitation to Resina Ferulae plant root N accumulation, and the promotion to 1a Resina Ferulae plant is made
With becoming apparent from of relatively 2a, bacterium solution W5 is more than W1 to the effect of Resina Ferulae root.
2.1.2 bacterium solution on 1, the impact of 2a Resina Ferulae root N stage accumulation
Bacterium solution W1, the W5 shadow to the nitrogen stage accumulation of Resina Ferulae root different growing stage
Ring, be shown in Table 2.Each nitrogen accumulation processed totally drops in first increasing afterwards along with the prolongation of growth time
Trend, and bacterium solution has facilitation to the N stage accumulation of different growing stage.1a nitrogen
Stage accumulation all 30～45d peakings after watering bacterium, CK, W1, W5 account for total amount respectively
60.52%, 63.57%, 58.53%, without significant difference between W1, W5, but all with compare
(CK) significant difference (P ＜ 0.05) between.2a nitrogen stage accumulation reaches watering bacterium 0～15d
Peak value, W1, W5 account for the 35.34% of total amount, 33.33% respectively, but relatively comparison (CK) carries
Front 15d.Illustrate that bacterium solution W1, W5 all can improve 1, the stage of the N of 2a Resina Ferulae root is amassed
Tired amount, and the stage accumulation peak value of 2a Resina Ferulae is ahead of time, the N maximum stage of 1a root accumulates
Amount accounts for the big of sum-rate substantially relatively 2a.
Table 2 bacterium solution on 1, the impact of 2a Resina Ferulae plant root N stage accumulation
2.1.3 bacterium solution on 1, the impact of 2a Resina Ferulae root N accumulation characteristic
Water bacterium process after, the individual plant accumulation (Y) of Resina Ferulae root N with emerge after natural law (X)
Logistic equation is used to be simulated (table 3).Result show correlation coefficient all 0.91 with
On, therefore fitting effect is preferable.1a Resina Ferulae root N Rapid Accumulation phase initial time is for emerging
Rear 41～73d, Rapid Accumulation phase △ t is 16～21d, absorption maximum speed Vm and Rapid Accumulation
Phase gross accumulation amount GT all shows as W5 > W1 > CK, and Vm the most relatively compares and adds
60.70%, 57.71%, GT the most relatively compare and add 28.81%, 24.39%.2a Resina Ferulae
Root N Rapid Accumulation phase initial time is after emerging 27～64d, and the Rapid Accumulation phase, △ t was
23～29d, absorption maximum speed Vm and Rapid Accumulation phase gross accumulation amount GT all show as
W5 > W1 > CK, Vm the most relatively compare and add 52.19%, 47.41%, and GT is the most right
According to adding 22.95%, 18.23%.Illustrate bacterium solution to 1,2a Resina Ferulae root N accumulation have rush
Entering effect, mainly N absorption rate Vm by increasing plant root increases the long-pending of it
Tired amount, and 1a Resina Ferulae N absorption rate is acted on more than 2a Resina Ferulae.
Table 3 bacterium solution to 1,2a Resina Ferulae root N accumulate Logistic equation simulation
2.2 bacterium solution to 1,2a Resina Ferulae root P2O5The impact of accumulation
2.2.1 bacterium solution to 1,2a Resina Ferulae root P2O5The impact of accumulation dynamic
After bacterium solution pouring, 1, the Resina Ferulae plant root P of 2a2O5After accumulation trend is all in first rising
Downward trend (Figure 11), wherein, when watering bacterium 15d, respectively processes plant P2O5Accumulation
All show as CK > W5 > W1, and all in significant difference (P ＜ 0.05).When watering bacterium 45d,
Each process root of Resina Ferulae portion P2O5Accumulation peaking, all shows W5 > W1 > CK.1a Resina Ferulae
P2O5Accumulation the most relatively compares and adds 20.45%, 13.64%, and W1, W5 and CK
Between all in significant difference (P ＜ 0.05).2a Resina Ferulae P2O5Accumulation the most relatively compares and adds
14.12%, 9.04%, between bacterium solution W1, W5 and CK all in significant difference (P ＜ 0.05).
Illustrate when bacterium solution processes 15d Resina Ferulae root P2O5Absorption have inhibitory action, the most right
Resina Ferulae plant root P2O5Accumulation has facilitation, and the obvious 2a of effect to 1a Resina Ferulae root
Resina Ferulae, bacterium solution W5 is more than W1 to the effect of Resina Ferulae root.
2.2.2 bacterium solution to 1,2a Resina Ferulae root P2O5The impact of stage accumulation
Each P processed2O5Accumulation totally declines in first rising afterwards along with the prolongation of growth time
Trend (being shown in Table 4), and bacterium solution all has rush to the phosphorus element stage accumulation of different growing stage
Entering effect, all 15～30d stage accumulation peakings after watering bacterium, relatively CK postpones 15d.
After W1, W5 process, 1a Resina Ferulae accounts for the 48.78% of integral dose, 42.04% respectively, bacterium
Significant difference is reached between liquid.2a Resina Ferulae accounts for the 42.72% of integral dose, 39.43% respectively, bacterium
Without significant difference between liquid.Illustrate bacterium solution W1, W5 all can improve 1,2a Resina Ferulae plant root
The P in portion2O5Stage accumulation, and 1, move, all after the stage accumulation peak value of 2a root
After watering bacterium, 15～30d reach to accumulate peak period.The P of 1a root2O5Maximum stage accumulation accounts for
Big (the P ＜ 0.05) of sum-rate substantially relatively 2a.
Table 4 bacterium solution to 1,2a Resina Ferulae root P2O5The impact of stage accumulation
2.2.3 bacterium solution to 1,2a Resina Ferulae root different times P2O5The impact of accumulation characteristic
After watering bacterium process, Resina Ferulae root P2O5Individual plant accumulation (Y) with emerge after natural law
(X) available Logistic equation is simulated (table 5).Result shows that correlation coefficient is all 0.94
Above, therefore fitting effect is preferable.1a Resina Ferulae root P2O5Rapid Accumulation phase initial time is
After emerging 47～56d, Rapid Accumulation phase △ t is 13～15d, absorption maximum speed Vm and fast
Speed accumulation phase gross accumulation amount GT shows as W5 > W1 > CK, Vm the most relatively compare and add
22.40%, 18.03%；GT the most relatively compares and adds 23.40%, 10.94%.2a Resina Ferulae
Root P2O5Rapid Accumulation phase initial time is after emerging 28～58d, and the Rapid Accumulation phase, △ t was
22～25d, absorption maximum speed Vm, Rapid Accumulation phase gross accumulation amount GT show as W5 >
W1 > CK, Vm the most relatively compare and add 10.15%, 6.00%, and GT the most relatively compares increasing
Add 20.38%, 10.88%.Illustrate bacterium solution to 1,2a Resina Ferulae plant root P2O5Accumulation has
Facilitation, mainly absorption maximum speed Vm by increasing plant root increase P2O5
Accumulation, and 1a Resina Ferulae P acquisition speed is acted on more than 2a.Along with Resina Ferulae is planted
The increase of strain Growing years, the suction phosphorus ability of Resina Ferulae plant root is increasingly stronger, fast increasing time phase
The longest.
Table 5 bacterium solution to 1,2a Resina Ferulae root P2O5Accumulation Logistic equation simulation
2.3 bacterium solution on 1, the impact of 2a Resina Ferulae root potassium element accumulation
2.3.1 bacterium solution to 1,2a Resina Ferulae root K2The impact of O accumulation dynamic change
After bacterium solution pouring, 1, the Resina Ferulae root potassium element of 2a accumulation trend the most first rises to decline afterwards and (scheme
, and show different difference 12,13).1,2a Resina Ferulae root K2When O is accumulated in 45d
Peaking, all shows as W5 > W1 > CK.The wherein K of 1a2O accumulation the most relatively compares increasing
Added 23.59%, between 14.23%, and W1, W5 and CK all in significant difference (P ＜ 0.05).
The K of 2a2O accumulation the most relatively comparison adds 17.69%, 10.88%, and W1, W5 and
In significant difference (P ＜ 0.05) between CK.Illustrate bacterium solution to Ah 1,2a Resina Ferulae root potassium element
Absorb and have facilitation with accumulation, the facilitation of 1a Resina Ferulae root is more than 2a, and bacterium
The facilitation of liquid W5 is more than W1.
2.3.2 bacterium solution to 1,2a Resina Ferulae root K2The impact of O stage accumulation
Bacterium solution W1, the W5 impact (table 6) on Resina Ferulae root potassium element stage accumulation.Everywhere
The K of reason2O accumulation along with growth time prolongation totally in first rise after downward trend,
And potassium element different growing stage stage accumulation is had facilitation.W1, W5 of 1a all exist
15～30d stage accumulation peakings, account for the 34.47% of integral dose, 36.43% respectively, relatively
CK moves forward 15d.2a Resina Ferulae is after watering W1, W5 bacterium, and stage accumulation reaches peak 0～15d
Value, accounts for the 31.31% of integral dose, 34.68% respectively, and relatively CK moves forward 15d.Bacterium solution is described
W1, W5 all can improve 1, the K of 2a Resina Ferulae root2The stage accumulation of O, bacterium solution W1,
W5 makes 1,2a Resina Ferulae root stage accumulation peak value all moves forward, i.e. 1a after watering bacterium 15～30d,
2a Resina Ferulae root is after watering bacterium 0～15d, and the maximum stage accumulation of 2a Resina Ferulae root potassium element
Account for sum-rate big compared with 1a Resina Ferulae.
Table 6 bacterium solution on 1, the impact of 2a Resina Ferulae root K2O stage accumulation
2.3.3 bacterium solution to 1,2a Resina Ferulae root K2The impact of O accumulation characteristic
After watering bacterium process, Resina Ferulae root K2The individual plant accumulation (Y) of O with emerge after natural law
(X) available Logistic equation is simulated (table 7).Result shows that correlation coefficient is all 0.92
Above, therefore fitting effect is preferable.1a root K2O Rapid Accumulation phase initial time is for emerging
Rear 35～62d, Rapid Accumulation phase △ t is 17～24d, and absorption maximum speed Vm is with the most long-pending
Tired phase gross accumulation amount GT all shows as W5 > W1 > CK, and Vm the most relatively compares and adds
51.72%, 33.33%, GT the most relatively compare and add 14.02%, 7.66%.2a root
K2O Rapid Accumulation phase initial time is 31-61d after emerging, and Rapid Accumulation phase △ t is 26～28d,
Absorption maximum speed Vm and Rapid Accumulation phase gross accumulation amount GT show as W5 > W1 > CK,
Vm the most relatively compares and adds 17.06%, 10.92%, and GT the most relatively compares and adds
14.22%, 8.82%.Illustrate bacterium solution to 1,2a Resina Ferulae root K2O accumulation has facilitation,
Mainly by increasing the K of root2O absorption maximum speed Vm increases its accumulation,
And bacterium solution is more than 2a Resina Ferulae to the impact of the potassium absorption speed of 1a Resina Ferulae.Along with Resina Ferulae is raw
The increase in long time, the absorbing potassium capability of Resina Ferulae root is increasingly stronger, and time Rapid Accumulation phase also gets over
Long.
Table 7 bacterium solution to 1,2a Resina Ferulae root K2O accumulate Logistic equation simulation
Research in terms of three above it can be concluded that
First aspect: bacterium solution has facilitation to Resina Ferulae plant above ground portion, the growth of root, and
Effect to overground part > root, the facilitation of bacterial strain W5 is more than W1.Along with Resina Ferulae children
The passage of Seedling growth time, Resina Ferulae total biomass reduces trend afterwards in first raising；Overground part dry
The apportionment ratio of matter accumulation presents downward trend, and root Dry Matter rate is in rising trend, waters
During bacterium 15d, the apportionment ratio increase of root dry-matter accumulation is the most obvious, relatively waters bacterium 0d and adds respectively
29.71% (CK), 30.28% (W1), 30.8% (W5), show Resina Ferulae plant root
Mainly initially entered vigorous period from May 1 during this period of time to the moon 15.
Second aspect: after watering bacterium solution, bacterial strain W1, W5 bacterium solution is to Resina Ferulae overground part malonaldehyde
(MDA) content the most relatively compares and increased, and shows to add the cell membrane damage of overground part
Weight, and root MDA content the most relatively compares and decreases, and shows the cell to Resina Ferulae root
Membrane damage slows down, and discloses bacterium solution and is conducive to the growth of Resina Ferulae root, thus it is speculated that to root resistance
Improve and have facilitation.Meanwhile, the facilitation of SOD, Proline is showed by bacterium solution
For root > overground part, the facilitation of POD, soluble protein is shown as overground part > root
The soluble sugar content of portion, overground part and root all decreases.Show that bacterium solution can be by carrying
High anti-oxidation thing enzyme (SOD, POD) activity and within the specific limits increase Proline and
The content of soluble protein, strengthens the degeneration-resistant border ability of self.
The third aspect: Pleurotus ferulae Lanzi bacterium solution substantially can improve Resina Ferulae root nutrition accumulation, stage
Accumulation, nutrient accumulation speed.Bacterium solution W1, W5 are to 1a Resina Ferulae root N, P2O5、K2O
Accumulation adds 42.44%～54.11%, 14.11%～21.27%, 14.23%～23.59% respectively,
To 2a root N, P2O5、K2O accumulation adds 21.14%～26.68% respectively,
9.24%～14.24%, 10.88%～17.69%.After watering bacterium solution, the stage accumulation of Resina Ferulae occurs
Change, 1a Resina Ferulae root N and 1,2a Resina Ferulae K2O accumulation peak value moves forward, 1,2a Ah
Wei P2O5Rear shifting, and increase root by absorption rate Vm improving Resina Ferulae seedling root
The accumulation of nutrient, 1a Resina Ferulae root N, P2O5、K2O absorption rate adds respectively
57.71%～60.70%, 18.03%～22.40%, 51.72%～33.33%, 2a Resina Ferulae root N,
P2O5、K2O accumulation adds 52.19%～47.41%, 6.00%～10.15% respectively,
10.92%～17.06%.As can be seen here, bacterium solution is to Resina Ferulae plant root N, P2O5、K2O amasss
Tired have facilitation, and bacterial strain W5 is more than bacterial strain W1 to Resina Ferulae seedling root facilitation, right
The summation of 1a Resina Ferulae root is more than 2a Resina Ferulae.
In sum, at farmland benign environment condition, Pleurotus ferulae Lanzi fungus and ermophyte Resina Ferulae
Action mode becomes Mutualism from pseudosymphile, and from resource distribution, host plant is by reproductive capacity
It is changed into existence, beneficially the growth of Resina Ferulae seedling.
Claims (8)
1. the method that ermophyte Resina Ferulae growth of seedling is affected by research Pleurotus ferulae Lanzi fungus bacterium solution, it is characterised in that first described method prepares Pleurotus ferulae Lanzi bacterium solution, to Resina Ferulae seedling in root trench digging pouring Pleurotus ferulae Lanzi bacterium solution, arranges matched group；Then the impact on Resina Ferulae growth of seedling of three aspect research Pleurotus ferulae Lanzi bacterium solution is passed through, first aspect, with Resina Ferulae seedling different parts dry matter weight, fresh weight and root length degree, a diameter of index of root, research Pleurotus ferulae Lanzi bacterium solution is on Resina Ferulae seedling dry biomass, fresh Biomass and the impact of root growth situation；Second aspect, analyzes and Resina Ferulae growth of seedling and degeneration-resistant relevant physical signs, and research Pleurotus ferulae Lanzi bacterium solution is on Resina Ferulae growth of seedling and the impact of resistance；The third aspect, with seedling root nutrition accumulation as index, research Pleurotus ferulae Lanzi bacterium solution is on Resina Ferulae seedling root Nutrient Absorption and the impact of accumulation.
The method that the most according to claim 1, ermophyte Resina Ferulae growth of seedling is affected by research Pleurotus ferulae Lanzi fungus bacterium solution, it is characterized in that, the research method of described first aspect is particularly as follows: with 1 year raw seedling as object of study, to the 0d after Resina Ferulae seedling root pouring Pleurotus ferulae Lanzi bacterium solution, 15d, 30d, 45d and 60d is separately sampled, after the Resina Ferulae seedling gathered is carried out pretreatment, measure Resina Ferulae seedling overground part and root dry matter weight, fresh weight and root length degree and root diameter, with research Pleurotus ferulae Lanzi bacterium solution to Resina Ferulae seedling dry biomass, fresh Biomass and the impact of root growth situation.
The method that the most according to claim 1, ermophyte Resina Ferulae growth of seedling is affected by research Pleurotus ferulae Lanzi fungus bacterium solution, it is characterized in that, the research method of described second aspect is particularly as follows: with 1 year raw seedling as object of study, separately sampled to the 0d after Resina Ferulae seedling root pouring Pleurotus ferulae Lanzi bacterium solution, 15d, 30d, 45d and 60d, after the Resina Ferulae seedling gathered is carried out pretreatment, measure Resina Ferulae seedling overground part and root and Resina Ferulae growth of seedling and degeneration-resistant relevant physical signs；Described include superoxide dismutase activity, peroxidase activity, mda content, free proline content, content of soluble protein, soluble sugar content to Resina Ferulae growth of seedling and degeneration-resistant relevant physical signs；The mensuration of superoxide dismutase activity uses nitroblue tetrazolium photochemical reduction method, the mensuration of peroxidase activity uses guaiacol method, the mensuration of free proline content uses sulfosalisylic acid colorimetric method, the mensuration of mda content uses thiobarbituric acid reaction method, the mensuration of content of soluble protein uses Coomassie brilliant G-250 staining, and the mensuration of soluble sugar content uses anthrone ethyl acetate method；Result is 3 meansigma methodss repeated.
The method that the most according to claim 1, ermophyte Resina Ferulae growth of seedling is affected by research Pleurotus ferulae Lanzi fungus bacterium solution, it is characterized in that, the research method of the described third aspect is particularly as follows: with 1 year raw seedling and 2 years raw seedling as object of study, separately sampled to the 0d after Resina Ferulae seedling root the last time pouring Pleurotus ferulae Lanzi bacterium solution, 15d, 30d, 45d and 60d, after the Resina Ferulae seedling gathered is carried out pretreatment, measure Resina Ferulae seedling root nutrition accumulation；Described Resina Ferulae seedling root nutrition accumulation includes that N accumulation, N stage accumulation, N stage accumulation account for sum-rate, P2O5Accumulation, P2O5Stage accumulation, P2O5Stage accumulation accounts for sum-rate, K2O accumulation, K2O stage accumulation, K2O stage accumulation accounts for sum-rate.
5. the method according to one of claim 2-4 described research Pleurotus ferulae Lanzi fungus bacterium solution, ermophyte Resina Ferulae growth of seedling affected, it is characterized in that, the Resina Ferulae seedling gathered is carried out pretreatment particularly as follows: Resina Ferulae seedling is divided into overground part and root, at 120 DEG C of 30 min that complete, then in 80 DEG C
Dry to constant weight, claim dry weight, grinding, put into valve bag preserves to be measured.
6. the method affected ermophyte Resina Ferulae growth of seedling according to one of claim 2-4 described research Pleurotus ferulae Lanzi fungus bacterium solution, it is characterised in that during sampling, randomly selects and grows fine and grow basically identical 10～20 strain Resina Ferulae seedling plants.
7. according to studying the method that ermophyte Resina Ferulae growth of seedling is affected by Pleurotus ferulae Lanzi fungus bacterium solution described in claim 1, it is characterized in that, the preparation method of described Pleurotus ferulae Lanzi bacterium solution is particularly as follows: under aseptic condition, PDA culture medium is cultivated Pleurotus ferulae Lanzi bacterial strain and covers with culture dish to Pleurotus ferulae Lanzi, the Pleurotus ferulae Lanzi bacterial strain card punch covering with culture dish is taken a diameter of 0.6cm truffle, described truffle is accessed equipped with in the triangular flask of fermentation medium, it is placed in 24 DEG C, in the shaking table of 120 r/min, cultivate 30d, bacterium solution is filtered, collect supernatant, i.e. obtain described Pleurotus ferulae Lanzi bacterium solution: in described fermentation medium, the concentration of each component is: Rhizoma Solani tuber osi 25g/L, glucose 20g/L, peptone 3g/L, potassium dihydrogen phosphate 2g/L, magnesium sulfate 1g/L.
8. according to studying the method that ermophyte Resina Ferulae growth of seedling is affected by Pleurotus ferulae Lanzi fungus bacterium solution described in claim 1, it is characterized in that, to Resina Ferulae seedling in root trench digging pouring Pleurotus ferulae Lanzi bacterium solution particularly as follows: the pouring of Pleurotus ferulae Lanzi bacterium solution uses ditch spread, the Resina Ferulae seedling of drilling is watered Pleurotus ferulae Lanzi bacterium solution at root trench digging, the irrigation amount of Pleurotus ferulae Lanzi bacterium solution controls at 0.5L/ rice row long, and matched group does not water Pleurotus ferulae Lanzi bacterium solution.
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