CN1720785B - An artificial high-yield cultivation method of male agaric and application of fruit body thereof - Google Patents
An artificial high-yield cultivation method of male agaric and application of fruit body thereof Download PDFInfo
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- CN1720785B CN1720785B CN 200410041704 CN200410041704A CN1720785B CN 1720785 B CN1720785 B CN 1720785B CN 200410041704 CN200410041704 CN 200410041704 CN 200410041704 A CN200410041704 A CN 200410041704A CN 1720785 B CN1720785 B CN 1720785B
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Abstract
The invention discloses a high yield method for the artificial cultivation of phellinus baum and the use of phellinus baum fruiting body, wherein the cultivation method comprises choosing mulberry branches or mulberry sawdust as the main culture medium, loading into bags or bottles, sterilizing, germ-free inoculating, culturing the fungus, finally harvesting the ripened fruiting body, which can be prepared into electuary and capsule.
Description
Technical field
The present invention relates to the preparation of a kind of Phellinus method of cultivating bacterial spawn and Phellinus bacterial classification fruit body.
Background technology
Through Primary Study, Phellinus has the effect of outstanding inhibition precancer cellular invasion, and is also very remarkable to hypertensive hypotensive effect.Behind cancer operation, Phellinus prevents that the effect of its recurrence is also very remarkable, can improve the immunologic function of human body; Phellinus and general anti-cancer chemotherapy are parallel when using, and can further improve result of treatment.
According to the data of present grasp, the wild at home areal concentration of Phellinus mainly contains: between the Wusuli River, east, Heilongjiang Province and Xinkaihu Lake, 1999～calendar year 2001 is produced more than 30 ton altogether; " Characteristics in Ziwuling " nature reserve that Shaanxi, the Northwest and Gansu have a common boundary produces about 50 tons from calendar year 2001～2003 year May altogether.Master mountain range between the Forest in Changbai Mountain Forest Region in northeast, Harbin and the Jilin, the Zhang Guangcai mountain range is also rare produces.In addition, each provinces and regions, southwest also produce a spot of mulberry tree gives birth to Phellinus, but output is few, is difficult to form commodity.
Because domestic and international market is big to the demand of Phellinus, the price height causes domestic each place of production source of goods exhaustion, and the fruit body spore can't form in a large number.Artificial cultivation Phellinus bacterium has become the task of top priority.
Summary of the invention
The purpose of this invention is to provide a kind of artificial high yield cultivation method and Phellinus fruit body preparation of Phellinus, make Phellinus to cultivate in a large number, satisfy medicine and health-product market needs, masses bring benefit to the people.
Technical scheme of the present invention is as follows:
Phellinus artificial high yield cultivation method selects Ramulus Mori or mulberry tree sawdust as the main medium material, behind pack or bottling, sterilization, the aseptic inoculation, sends out bacterium in culturing room then; After mycelia is covered with, during cultivating in bag, in order to going out sesame, in going out the sesame canopy, become wall type to put with blade handle bag two ends scarfing; Plant as bottle and then to open bottle stopper, be placed on the stereoscopic culture frame; The canopy temperature remains on 18～26 ℃ when going out sesame, and relative air humidity brings up to 90～95%, and scattered light and sufficient oxygen are provided, and keeps ventilating; Original hase expanded 3 days～5 days, formed cap gradually, increased water spray and preserved moisture, and Phellinus fruit body maturation can in time be gathered.
Described Phellinus artificial high yield cultivation method is characterized in that the each component weight proportion of medium is:
Ramulus Mori or mulberry tree sawdust 75～85 wheat skins 8～12 rice brans 4～6 sucrose 1～3
Magnesium sulfate 0.4～0.6 composite fertilizer (N14-P10-K14) 0.4～0.6
Gypsum 1～3
Growth hormone 0.04～0.06
The water content of medium is 60%～70%;
Wherein the each component weight proportion of growth hormone is:
6-benzyl aminopurine 0.8～1.2
Methyl 45～55
Indolebutyric acid 1～3
Gibberellin 1～3
Distilled water 1000.
Described Phellinus artificial high yield cultivation method is characterized in that the scarfing at described bag two ends is that diameter is 1.5～2.5 centimetres a round mouth.
Described Phellinus artificial high yield cultivation method; after it is characterized in that mycelia is covered with in the bacterium bag; openning about one 6～12 centimetres is cut with the insert pocket of sterilizing in bacterium bag bottom; and then will be placed in the pond down at the bottom of the bag uniformly; cover the sand of the sterilization of 8～12 cm thicks in the pond; go out Zhi Kou with opening 4～6 equably around the bacterium bag of the blade of sterilizing above sand bed Deng the mycelia recovery again after 2～3 days, then sand is irritated sufficient water and promptly can go out sesame.
The application of described artificial cultivation Phellinus fruit body is characterized in that Phellinus fruit body water is decocted, and collects decocting liquid, the precipitation removal of impurities, and concentrating under reduced pressure becomes paste again, and crushed after being dried becomes fine powder, makes the Phellinus capsule.
The application of described artificial cultivation Phellinus fruit body is characterized in that Phellinus fruit body water is decocted, and collects decocting liquid, and the precipitation removal of impurities concentrates adding preservative agent with decocting liquid and makes the Phellinus oral liquid.
The application of described artificial cultivation Phellinus fruit body is characterized in that Phellinus fruit body water is decocted, and collects decocting liquid, the precipitation removal of impurities, and concentrating under reduced pressure becomes paste again, and crushed after being dried becomes fine powder, adds auxiliary material and makes the Phellinus electuary.
The application of described artificial cultivation Phellinus fruit body is characterized in that Phellinus fruit body water is decocted, and collects decocting liquid, and the precipitation removal of impurities adds alcohol extract again, collects its sediment decompression oven dry, makes the Phellinus polyoses capsule.
Phellinus cultivation method of the present invention is simple, can be used for artificial a large amount of cultivation, and obtain the Phellinus fruit body, has overcome the deficiency of wild resource.
The Phellinus fruit body preparation of the present invention's preparation has sharp the five internal organs, a surname's intestines gas, hemostasis, soft heavily fortified point, functions of expelling toxin.To the canceration of digestive system, as cancer of the stomach, cancer of the esophagus, duodenal cancer, colon cancer, rectum, combined effect is obvious when reaching the operation of liver cancer chemotherapy, and good raising body immunity function is arranged, and is used for the treatment of AIDS and also obtains good effect.
Embodiment
Example 1:
The domestication of wild Phellinus strain is cultivated:
Since 1998; respectively from Heilungkiang, ground collections such as Gansu, Shaanxi and Dabie Mountain are grown in more than ten of wild Phellinus fruit bodys on different regions and the seeds; 5 of the mycelium that process is separated and the acquisition of purifying can be grown; again through for many years domestication's rejuvenation, obtain 2 can normal growth be grown under the artificial environment of creating the Phellinus new varieties.
The fast culture of Phellinus bacterial classification:
Because of being subjected to the influence of environment and culture, mycelial growth is slow at artificial planting for wild Phellinus, cultivates the bacterial classification needs and just can cover with in about 60 days.The applicant adds growth hormone (prescription is listed hereinafter) and succeeds in strain culture-material, phellinus liteus can be grown fast, just can cover with in general 30 days, and the time shortens more than 50%.
Phellinus artificial high yield culture technique:
The applicant shows that Phellinus is to grow through years of researches in numerous trees sawdust cultures, but very slowly, and can not form fruit body.The applicant is through hundreds of inferior formula tests, and discovery can only just be grown in Ramulus Mori or mulberry tree sawdust easily, forms fruit body.The applicant further studies, and has obtained better culture medium prescription.
Adopt following prescription medium (kilogram):
Ramulus Mori or mulberry tree sawdust 80 wheat skins 10 rice brans 5 sucrose 2
Magnesium sulfate 0.5 composite fertilizer (N14-P10-K14) 0.5 gypsum 2
Growth hormone 0.05
The water content of medium is 60%～70%;
Wherein the each component weight proportion of growth hormone is (gram):
6-BA (6-benzyl aminopurine) 1
NAA (methyl) 50
IBA (indolebutyric acid) 2
920 (gibberellin) 2
Distilled water 1000.
Dissolve and cool off after each component is placed on distilled water heating, it is standby to be placed in the refrigerator preservation.
Composite fertilizer (N14-P10-K14) is meant common composite fertilizer, wherein contains nitrogen 14%, phosphorus 10%, potassium 14% (percentage by weight).
With medium pack or bottling, sterilize then, aseptic inoculation is sent out bacterium in the culturing room, and mycelia can be covered with composts or fertilisers of cultivating in one month.After mycelia was covered with, bag was planted with blade two ends is slit into the circular port of 5 coin-dividing sizes, and the one-tenth wall type is put in order to going out sesame in the sesame canopy.The canopy temperature remains on 18～26 ℃ when going out sesame, and relative air humidity brings up to 90～95%, and scattered light and sufficient oxygen are provided.Keep ground to have shoaling layer, around wall, reach space water spray 3～4 times every day.8 o'clock every mornings reached at 4 o'clock in afternoon in the past with back pullout (BPO) and ventilating opening ventilation, ventilation at 12 o'clock～14 o'clock noon when temperature is low.Original hase expanded 3 days～5 days, formed cap gradually, increased water spray and preserved moisture (once containing 0.1% growth hormone solution every spray in 3 days can make the quick growth of fruit body expand) the too high temperature control of will spraying water of temperature.Improper ventilation easily goes out lopsided sesame, will in time slice off when abnormal bud occurring.Cap is by---pale yellow---yellowish-brown in vain, and cap edge white is basic to disappear, the edge flavescence, and cap begins keratinization, and eject when filemot at the back side, shows that the Phellinus fruit body is ripe, can in time gather.Gathering from scarfing to the fruit body maturation generally only need about 50 days (wild needs 1～2 year).
Bottle is planted technology: the bottle that mycelia is covered with is placed on the stereoscopic culture frame (administrative skill that goes out behind the sesame is planted the same with harvesting technique with bag).
The Bag Material soil covering cultivation technology: the bacterium bag that mycelia is covered with, cut openning about one 10 centimetres with bacterium bag bottom with the insert pocket of sterilize when putting, and then be placed in down in the pond bottom uniform, the sand of the sterilization of 10 centimetres of coverings in the pond.Recover after 3 days Deng mycelia that opening 4～6 equably goes out Zhi Kou at the bacterium bag with the blade of sterilize more all around, then sand is irritated sufficient water and promptly can go out sesame, the management method that goes out behind the sesame is planted the same for same bag with harvesting technique.Keep mycelia moisture in the composts or fertilisers of cultivating owing to adopt earthing to play, the Phellinus fruit body is bigger than the length that does not have earthing, and quality is good, can improve 30～40% by repeatedly contrasting output.
Several Phellinus artificial cultivation technique of the present invention can several times a year carry out artificial cultivation.
Example 2:
The hot water extracting technology of Phellinus active ingredient:
The Phellinus fruit body is by crusher in crushing or be cut into 0.5～1 centimetre fragment, throws in stainless steel extracts pot, and the adding water yield is about 14 times of Phellinus weight.The back heating makes the water boiling, keeps boiling about 2 hours, filters to lift one's head with 80 mesh sieves and fries in shallow oil (decocting liquid), and the Phellinus slag is still stayed in the pot, adds 12 times of water gagings again, seethes with excitement about 2 hours, still filters out two with 80 mesh sieves and fries in shallow oil (decocting liquid).The secondary decocting liquid is merged, input storage liquid bath, quiescent setting 12 hours (also can use filter method) is got supernatant, removes the bottom slurry.The extract that obtains is called hot water extracting liquid.Phellinus hot water extracting liquid is heating (60～70 ℃) through decompression, and evaporation and concentration is surveyed density 1: 1.35～1.38 to heat, and (also can use other method for concentration) makes it to become paste, and the Phellinus paste active ingredient of acquisition is after super-dry, and abrasive dust is standby.The active ingredient of this extraction can be widely used in the preparation of Phellinus medicine etc.
Example 3:
Phellinus capsule manufacture technology
The Phellinus fruit body is by crusher in crushing or be cut into 0.5～1 centimetre fragment, throws in stainless steel extracts pot, and the adding water yield is about 14 times of Phellinus weight.The back heating makes the water boiling, keeps boiling about 2 hours, filters to lift one's head with 80 mesh sieves and fries in shallow oil (decocting liquid), and slag is still stayed in the pot, adds 12 times of water gagings again, seethes with excitement about 2 hours, still filters out two with 80 mesh sieves and fries in shallow oil (decocting liquid).The secondary decocting liquid is merged, input storage liquid bath, quiescent setting 12 hours (also can use filter method) is got supernatant, removes the bottom slurry.Clear liquid is concentrated again, and abrasive dust is made the Phellinus capsule after the Phellinus active ingredient drying of acquisition.
Example 4:
The production technology of Phellinus oral liquid:
The Phellinus fruit body is by crusher in crushing or be cut into 0.5～1 centimetre fragment, throws in stainless steel extracts pot, and the adding water yield is about 14 times of Phellinus weight.The back heating makes the water boiling, keeps boiling about 2 hours, filters to lift one's head with 80 mesh sieves and fries in shallow oil (decocting liquid), and slag is still stayed in the pot, adds 12 times of water gagings again, seethes with excitement about 2 hours, still filters out two with 80 mesh sieves and fries in shallow oil (decocting liquid).The secondary decocting liquid is merged, input storage liquid bath, quiescent setting 12 hours (also can use filter method) is got supernatant, removes the bottom slurry.Clear liquid is concentrated again allotment, can, sterilization packaging.
Example 5:
The production technology of Phellinus electuary:
Yellow fruit body is by crusher in crushing or be cut into 0.5～1 centimetre fragment, throws in stainless steel extracts pot, and the adding water yield is about 14 times of Phellinus weight.The back heating makes the water boiling, keeps boiling about 2 hours, filters to lift one's head with 80 mesh sieves and fries in shallow oil (decocting liquid), and slag is still stayed in the pot, adds 12 times of water gagings again, seethes with excitement about 2 hours, still filters out two with 80 mesh sieves and fries in shallow oil (decocting liquid).The secondary decocting liquid is merged, input storage liquid bath, quiescent setting 12 hours (also can use filter method) is got supernatant, removes the bottom slurry.Clear liquid is concentrated again, oven dry, the dry Phellinus active ingredient of reentrying is carried out abrasive dust, adds auxiliary material sterilization packagings such as carbohydrate.
Example 6:
The production technology of extraction of Phellinus polysaccharide and Phellinus polyoses capsule:
It is 1: 1.06～1.08 (heat is surveyed) that the water extraction solution of Phellinus is evaporated to density.Static about 12 hours, discard the bottom precipitation thing, get supernatant, add the edible ethanol of 95% concentration of 3 times of amounts of supernatant volume then, the limit edged stirs, if static again 24 hours, the bottom precipitation thing is the Phellinus polysaccharide.Pour out supernatant, with the centrifugal dehydration of bottom precipitation thing, 60 degree decompression oven dry have just formed Phellinus polysaccharide raw material.The Phellinus polysaccharide sieves about 80 orders through pulverizing, and the fine powder of gained is packed in the capsulae vacuus, has just become the Phellinus polyoses capsule.
Also containing main component in the supernatant of pouring out is the Phellinus oligosaccharides.Supernatant can be made into injection behind the desuperheating original shape thing, oral liquid or beverage through further purifying.Also can reclaim through ethanol, the decompression oven dry, abrasive dust is made the oligosaccharides capsule.
Claims (2)
1. Phellinus artificial cultivation method behind medium pack or bottling, sterilization, aseptic inoculation, is sent out bacterium in culturing room; After mycelia is covered with, during cultivating in bag, in order to going out sesame, in going out the sesame canopy, become wall type to put with blade handle bag two ends scarfing; Bottle is planted and is then opened bottle stopper, is placed on the stereoscopic culture frame; The canopy temperature remains on 18-26 ℃ when going out sesame, and relative air humidity is brought up to 90-95%, and scattered light and sufficient oxygen are provided, and keeps ventilating; Original hase expanded 3-5 days, formed cap gradually, increased water spray and preserved moisture, and Phellinus fruit body maturation can in time be gathered;
The each component weight proportion of above-mentioned medium is:
Ramulus Mori or mulberry tree sawdust 75-85 wheat skin 8-12 rice bran 4-6 sucrose 1-3
Magnesium sulfate 0.4-0.6
Composite fertilizer 0.4-0.6
Gypsum 1-3
Growth hormone 0.04-0.06
Described composite fertilizer is meant common composite fertilizer, wherein contains nitrogen 14%, phosphorus 10%, potassium 14% by weight percentage
The water content of medium is at 60-70%
Wherein the each component weight proportion of growth hormone is:
6-benzyl aminopurine 0.8-1.2
Methyl 45-55
Indolebutyric acid 1-3
Gibberellin 1-3
Distilled water 1000.
2. Phellinus artificial cultivation method according to claim 1 is characterized in that the scarfing at described bag two ends is that diameter is 1.5-2.5 centimetre a round mouth.
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Families Citing this family (15)
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| CN100399877C (en) * | 2006-02-24 | 2008-07-09 | 崔澎 | Cultivation of selenium enriched morin and method of extracting morin selenium polysacharide |
| CN102523909A (en) * | 2010-12-28 | 2012-07-04 | 秦绍新 | Method for planting phellinus igniarius by utilizing imitation cut-logs of mulberry twigs |
| CN102628064B (en) * | 2012-04-16 | 2013-12-04 | 山东省农业科学院农业资源与环境研究所 | Method for increasing yield of phellinus linteus total triterpene compound |
| CN103044229A (en) * | 2012-12-15 | 2013-04-17 | 青岛农业大学 | Technology for separating acetophenone from Phellinus |
| CN103053326B (en) * | 2012-12-28 | 2015-02-18 | 崇左市江州区生产力促进中心 | Method for cultivating phellinus igniarius using mulberry branch |
| CN103319618B (en) * | 2013-06-21 | 2016-06-29 | 上海市农业科学院 | The preparation method of active polysaccharide of male agaric mycelium |
| CN104350939A (en) * | 2014-09-18 | 2015-02-18 | 淳安千岛湖桑都食用菌专业合作社 | Cultivation method for phellinus igniarius manual fungus stick |
| CN104871821B (en) * | 2015-06-01 | 2017-10-17 | 四川省农业科学院土壤肥料研究所 | A kind of Phellinus strain and its cultural method |
| CN105060943A (en) * | 2015-09-02 | 2015-11-18 | 常州市长宇实用气体有限公司 | Preparation method of organic selenium nutrient solution |
| CN106072553A (en) * | 2016-06-29 | 2016-11-09 | 江荧 | A kind of manufacture method of Phellinus igniarius (L. ex Fr.) Quel. compound health cream |
| CN107496438B (en) * | 2017-10-13 | 2023-10-24 | 武汉轻工大学 | Application of Phellinus linteus polysaccharide in preparing medicine and health food |
| CN107550925A (en) * | 2017-10-13 | 2018-01-09 | 武汉轻工大学 | Application of the Phellinus polysaccharide in medicine and health food is prepared |
| CN109452297A (en) * | 2018-11-22 | 2019-03-12 | 佛山科学技术学院 | A kind of composite plant growth regulator and its application in raising autumn eggplant percentage of seedgermination |
| CN112189504A (en) * | 2020-08-28 | 2021-01-08 | 徐州工程学院 | Artificially-cultivated phellinus igniarius extract for treating migraine and preparation method thereof |
| CN113597972B (en) * | 2021-08-30 | 2022-12-13 | 陕西省微生物研究所 | Phellinus igniarius cultivation method |
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