CN107027519B - The mycorrhiza fungi seeding cultivating method of Panzhihua ferfas - Google Patents
The mycorrhiza fungi seeding cultivating method of Panzhihua ferfas Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The invention belongs to technical field of plant cultivation, and in particular to a kind of bacterium mycorrhiza fungi seeding cultivating method of Panzhihua ferfas.Aiming at the problem that prior art lacks the method for Panzhihua ferfas progress mycorrhiza fungi seeding cultivating, the present invention provides a kind of mycorrhiza fungi seeding cultivating method of Panzhihua ferfas, comprising the following steps: the collection and preservation of Panzhihua ferfas strain, the cultivation with the Va Mycorrhiza Seedling after when preparation method, pecan tree seed collection and preservation, the cultivation of pecan tree aseptic seedling, inoculation, inoculation Panzhihua ferfas of Panzhihua ferfas microbial inoculum.The present invention develops the inoculation that pecan tree is used for Panzhihua ferfas, compensates for the vacancy of areas of higher temperature cultivation truffle mycorrhiza fungi seeding economic fruit；It is formed and is matched using distinctive ferfas microbial inoculum, so that mycorhiza infection rate is greater than 85% after microbial inoculum inoculation, greatly improve plant percent.The method of the present invention is easy to operate, and infection rate is high, and transplanting survival rate is high, has significant economic benefit.
Description
Technical field
The invention belongs to technical field of plant cultivation, and in particular to a kind of mycorrhiza fungi seeding cultivating method of Panzhihua ferfas.
Background technique
Ferfas (T.ruffles) is ascomycetes Tuberale (Tuberales) Tuberaceae (Tuberaceae) Tuber
(Tuber), it is symbiosis edible mushroom most precious in the world, there is peculiar fragrance and nutritive value, it is international because of its limits throughput
Market at prices is expensive, and supply falls short of demand, is known as " black diamond ".
Panzhihua ferfas (T.panzhihuanense) fructification is very large, and single weight has fragrant, meat up to 450g or more
Matter is fine and smooth, good in taste, is the white blocks of the domestic great commercial value and larger business development potentiality found till now
Bacterium new varieties.The Panzhihua ferfas internal price of 3cm or more is in 1500 yuan of kg~1~2 000 yuan of kg~1, much higher than print
Spend ferfas (500 yuan of kg~1~800 yuan of kg~1).But Panzhihua ferfas Yield comparison is limited, and supply falls short of demand in market.
In order to improve the yield of Panzhihua ferfas, effective protection existing resource realizes Panzhihua ferfas sustainable utilization,
It can be by carrying out Panzhihua ferfas artificial cultivation.But Panzhihua ferfas artificial cultivation is relatively difficult, it is with high content of technology.Climb branch
The key of flower ferfas artificial cultivation is the cultivation of Panzhihua truffle mycorrhiza fungi seeding, and ferfas growth must be with suitable host plant root
Battalion, system commensalism, otherwise will be unable to form ascocarp, this means that artificial cultivation ferfas be unable to do without living body host plant.Block
The successful key of bacterium artificial cultivation is to first have to for ferfas to be inoculated into suitable host plant root, and ferfas mycelium germination simultaneously infects
Root system of plant forms symbiosis with plant, obtains Va Mycorrhiza Seedling.Again by Va Mycorrhiza Seedling plantation into suitable environment, the bacterium of ferfas
Silk can be grown with plant-root growth, and after the mycorhiza of ferfas grows to certain amount, the extension mycelia on mycorhiza surface will be turned round
It ties and starts fruiting.It is usually required 5~8 years from the time for being inoculated into fruiting, primary, sustainable receipts can be harvested after fruiting every year
It obtains 30~50 years.
Panzhihua ferfas is the white ferfas novel species of most commercial promise, and there is presently no mature Panzhihua ferfas people
Work cultural method.In existing India truffle and summer truffle mycorrhiza fungi seeding cultivating method, usually have solid fungicide and liquid bacterial agent into
Row inoculating two kinds method, solid fungicide inoculation generally first cultivate sterile bare-root seeding, and when inoculation replaces nutritive cube, there is one section after inoculation
The seedling-slowing stage of time, this will lead to inoculation seedling death rate height, influences seedling growth, mycorhiza is delayed to be formed.Liquid bacterial agent inoculation side
Method inoculum concentration is uneven, ferfas spore have it is more, have it is few, formed after inoculation mycorhiza quality is irregular in addition part nursery stock not
It can infect and form mycorhiza.
Pecan tree [Carya illinoensis (Wangenn.) K.Koch] originates in the U.S., is four big hard in the world
One of fruit.Pecan tree shell is thin, and kernel-taking is easy, and kernel percent 50% -70%, oil content 70~80%, unsaturated fatty acid contains
Amount 60% or so, unsaturated fatty acid and higher than olive to the beneficial various amino acid of human body.4 after pecan tree definite value
~5 years beginning fruitings, 10 years start to abound with, and period of high birth 50~70 years, 100-250kg/ mus, 6~8 dollars on international market/
Kg, domestic 60~120 yuan/kg of supermarket are 4~6 times of common walnut, are only calculated according to minimum output and lowest price, per mu yield
Value can reach 6000 yuan.
Pecan tree is in 15-20 DEG C of samming of year, 800~1600mm of rainfall, 1 5-10 DEG C of monthly average temperature, 7 monthly average temperature
20~30 DEG C, frost-free period 220~330 days, the area of P in soil H value 6~8 can plant, and be a kind of unique suitable low altitude area cultivation
Walnut Cultivars, low altitude area region is suitble to pecan tree cultivation in Panzhihua.Ferfas is 1100~2600m's of Panzhihua height above sea level
Range is distributed, and needs to carry out Panzhihua for the maximization for realizing pecan tree benefit according to the principle of " suitable to set suitable bacterium "
The breeding method of ferfas inoculation pecan tree Va Mycorrhiza Seedling.
Summary of the invention
The technical problem to be solved in the present invention are as follows: the prior art lacks the method for Panzhihua ferfas progress mycorrhiza fungi seeding cultivating
Problem.
The technical solution of present invention solution technical problem are as follows: a kind of mycorrhiza fungi seeding cultivating method of Panzhihua ferfas is provided.It should
Method the following steps are included:
A, the collection and preservation of Panzhihua ferfas strain
Panzhihua ferfas mature sporophore is collected, is ground into powder after disinfection；
B, Panzhihua ferfas microbial inoculum with when preparation method
The powder that step a is prepared and agstone, vermiculite power, turf powder, EM saccharomycete and bacillus subtilis
It is matched, is uniformly mixed by weight 30~35 ﹕, 8~12 ﹕, 20~25 ﹕, 10~15 ﹕, 1~2 ﹕ 1~2；
C, pecan tree seed collection and preservation
Pecan tree seed is collected, 5~7d is impregnated after disinfection；
D, the cultivation of pecan tree aseptic seedling
Hickory nut seed after immersion is seeded into the seedlings nursing plate equipped with sterilization matrix, addition 2~3cm sterilization matrix is covered
Lid seed, sprinkles profoundly water, and is placed in greenhouse and cultivates；
E, it is inoculated with
After the pecan tree culture of step d carries out 3 months, the step b microbial inoculum prepared is inoculated in seedlings nursing plate, often
Strain aseptic seedling is inoculated with 5~8g of ferfas microbial inoculum, then covers 2~3cm sterilization matrix, sprinkles profoundly water；
F, the cultivation of Arbuscular Mycorrhizal Fungi seedling
The hickory nut plant of the inoculated ferfas of step e is cultivated 4~6 months in greenhouse, obtains Panzhihua truffle mycorrhiza fungi
Seedling.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, the concrete operations of disinfection described in step a are with 70
~75% 3~5min of alcohol or hydrogen peroxide dipping.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, diameier≤0.5mm described in step a.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, limestone powder described in step b, vermiculite power,
The granularity of turf powder is≤0.5mm.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, the processing mode of disinfection described in step c are as follows: use
0.3~0.5% 3~5h of potassium permanganate liquid immersion treatment.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, immersion described in step c is that clear water impregnates, daily
It changes water 1~2 time.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, sterilization matrix described in step d is by weight grass
Charcoal soil: vermiculite: perlite: agstone=2~3 ﹕, 1~2 ﹕, 1~2 ﹕ 1~1.5 is mixed.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, the sterilization process of sterilization matrix described in step d is
It sterilizes 2~3 hours at 121~126 DEG C.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, the sterilization matrix of loading described in step d accounts for nursery
The 1/2~2/3 of disk depth.
Wherein, it in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, is fallen in seedlings nursing plate described in step d equipped with 4~8
Root line.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, the step d, condition of culture described in e are as follows: matrix
Humidity 25~35%, 20~30 DEG C of greenhouse temperature, greenhouse humidity 60~80%.
The invention has the benefit that the present invention provides a kind of mycorrhiza fungi seeding cultivating of Panzhihua ferfas inoculation pecan tree
Method, pecan tree compensate for areas of higher temperature cultivation economic fruit truffle mycorrhiza fungi seeding for the inoculation of Panzhihua ferfas
Vacancy；The distinctive microbial inoculum composition of the present invention and proportion are cultivated so that microbial inoculum inoculation postoperative infection rate is up to 90% using seedlings nursing plate,
The line of falling root is housed, so that fibrous root is more and growth distribution is uniform, mycorhiza quantity is more, and disk is not moved after inoculation, greatly improves nursery stock in disk
Survival rate, and without seedling-slowing stage.The method of the present invention is easy to operate, and infection rate is high, and transplanting survival rate is high, has significant economic effect
Benefit.
Figure of description
Attached drawing 1 show nonvaccinated pecan tree root；
Attached drawing 2 show the pecan tree mycorhiza after embodiment 1 is inoculated with；
Attached drawing 3 show the extension mycelia after embodiment 1 is inoculated on Va Mycorrhiza Seedling；
Attached drawing 4 show the Hartig net knot of mycorhiza surface layer horse-hof shape after embodiment 1 is inoculated with.
Specific embodiment
The present invention provides a kind of mycorrhiza fungi seeding cultivating methods of Panzhihua ferfas, comprising the following steps:
A, the collection and preservation of Panzhihua ferfas strain
Panzhihua ferfas mature sporophore is collected, with 70~75% 3~5min of alcohol or hydrogen peroxide dipping, is ground into straight
Diameter≤0.5mm powder；
B, Panzhihua ferfas microbial inoculum with when preparation method
The powder that step a is prepared and agstone, vermiculite power, turf powder, EM saccharomycete and bacillus subtilis
It is matched, is uniformly mixed by weight 30~35 ﹕, 8~12 ﹕, 20~25 ﹕, 10~15 ﹕, 1~2 ﹕ 1~2；
C, pecan tree seed collection and preservation
Pecan tree seed is collected, 5~7d is impregnated after disinfection；
D, the cultivation of pecan tree aseptic seedling
Hickory nut seed after immersion is seeded into the seedlings nursing plate equipped with sterilization matrix, addition 2~3cm sterilization matrix is covered
Lid seed, sprinkles profoundly water, and is placed in greenhouse and cultivates；
E, it is inoculated with
After the pecan tree culture of step d carries out 3 months, the step b microbial inoculum prepared is inoculated in seedlings nursing plate, often
Strain aseptic seedling is inoculated with 5~8g of ferfas microbial inoculum, then covers 2~3cm sterilization matrix, sprinkles profoundly water；
F, the cultivation of Arbuscular Mycorrhizal Fungi seedling
The hickory nut plant of the inoculated ferfas of step e is cultivated 4~6 months in greenhouse, obtains Panzhihua truffle mycorrhiza fungi
Seedling.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, limestone powder described in step b, vermiculite power,
The granularity of turf powder is≤0.5mm.
The present invention selects limestone powder, vermiculite power, turf powder and EM saccharomycete and Panzhihua ferfas to be used cooperatively, lime
Stone powder can make strain keep drying, moreover it is possible to provide the calcareous of ferfas growth, adjust pH value；Vermiculite power can maintain microbial inoculum
Gas porosity plays filling effect；Turf contains humic acid, can promote ferfas spore germination, promotes plant root growth；EM yeast
Bacterium and bacillus subtilis are conducive to the synthesis of truffle mycorrhiza fungi, can adjust the balance of Soil Microorganism group, improve ferfas bacterium
Root infection rate；Above-mentioned raw materials are mixed by weight 30~35 ﹕, 8~12 ﹕, 20~25 ﹕, 10~15 ﹕, 1~2 ﹕ 1~2, it can be with
Mycorhiza infection rate is set to reach 90% or more, 98% or more the survival rate of nursery stock, high survival rate of emerging.Select such ratio strain
Holding time is longer, and the germination rate of strain miospore is high, and easily infection nursery stock forms mycorhiza.
The limestone powder, vermiculite power, turf powder granularity preferably≤0.5mm, can make spore preferably from
Ascus separate out is easier to be uniformly dispersed in the root of sapling in inoculation.It is 30~35 ﹕ 8 by the limited proportion of three
~12 ﹕ 20~25, can make the holding time of microbial inoculum more long, and more loose ventilative, adhesiveness is good, and inoculation postoperative infection rate is high.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, the processing mode of disinfection described in step c are as follows: use
0.3~0.5% 3~5h of potassium permanganate liquid immersion treatment.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, immersion described in step c is that clear water impregnates, daily
It is primary to change water.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, sterilization matrix described in step d is by weight grass
Agstone=2 Tan Tu ﹕ Zhi Shi ﹕ Zhen Zhu Yan ﹕~3 ﹕, 1~2 ﹕, 1~2 ﹕ 1~1.5 is mixed.Turf contains humic acid, can
Promote ferfas spore germination, promotes plant root growth；Vermiculite can maintain the gas porosity of microbial inoculum, provide the various of plant growth
Minerals；Perlite plays filling effect, and loose matrix increases the permeable of matrix, gas permeability and water-retaining property；Agstone can provide
Calcareous, the adjusting matrix pH value of ferfas growth.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, the sterilization process of sterilization matrix described in step d is
It sterilizes 2~3 hours at 121~126 DEG C.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, the sterilization matrix of loading described in step d accounts for nursery
The 1/2~2/3 of disk depth.
Wherein, it in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, is fallen in seedlings nursing plate described in step d equipped with 4~8
Root line.On the one hand the line of falling root can promote fibrous root homoepitaxial, keep fibrous root quantity more, and be evenly distributed, and on the other hand, can make
Root is radially grown, and will not be spiraled, and transplanting survival rate is high.
Wherein, in the mycorrhiza fungi seeding cultivating method of above-mentioned Panzhihua ferfas, the step d, condition of culture described in e are as follows: matrix
Humidity 25~35%, 20~30 DEG C of greenhouse temperature, greenhouse humidity 60~80%.
The present invention selects pecan tree to carry out the cultivation of Va Mycorrhiza Seedling, and it is suitable in temperature that hickory nut, which is a kind of economic fruit,
Higher ecological region planting adapts to hot environment, and have compared to the existing economic tree Chinese chestnut for being inoculated with ferfas, fibert etc.
It is inoculated with conducive to the mycorhiza of Panzhihua ferfas, rate of vaccination is high, improves the benefit of ferfas plantation, has filled up areas of higher temperature cultivation
The vacancy of economic fruit truffle mycorrhiza fungi seeding.
Breeding method of the present invention understands, simply, and at low cost, and inoculation ferfas infection is fast, and mycorhiza infection rate is high, no slow seedling
Phase, no dead seedling, truffle mycorrhiza fungi seeding growth are neat.To realize that Panzhihua ferfas artificial cultivation has established solid foundation, provide
The safeguards technique of high-quality Va Mycorrhiza Seedling, the success of Panzhihua ferfas artificial cultivation not only can be with the existing Panzhihua ferfas moneys of effective protection
Source also meets the material life demand of the growing high-quality of the mankind, has a vast market foreground.
Explanation is further explained to a specific embodiment of the invention below by embodiment, but does not indicate to send out this
Bright protection scope is limited in range described in embodiment.
EM saccharomycete in the embodiment of the present invention is purchased to Shanxi Chang Tai animal pharmaceutical estate Co., Ltd, and execution standard is Q/
SCY010-213；Bacillus subtilis is purchased to Wuhan Nature, and product standard card number is Q/WTH05-
2012；
Panzhihua ferfas is purchased to Panzhihua Yi ferfas Co., Ltd.
Embodiment 1 carries out mycorrhiza fungi seeding cultivating to Panzhihua ferfas with the method for the present invention
Panzhihua truffle mycorrhiza fungi seeding cultivating, specific steps are as follows:
A, the collection and preservation of Panzhihua ferfas strain
Collect Panzhihua ferfas mature sporophore, after 70% alcohol or hydrogen peroxide dipping 3min, be ground into diameter≤
The powder of 0.5mm；
B, Panzhihua ferfas microbial inoculum with when preparation method
The ferfas powder and limestone powder, vermiculite power, turf powder, EM saccharomycete and withered grass bud that step a is prepared
Spore bacillus is matched by weight 30 ﹕, 8 ﹕, 20 ﹕, 10 ﹕, 1 ﹕ 1, is uniformly mixed；The limestone powder, vermiculite power, turf powder
Granularity be≤0.5mm；
C, pecan tree seed collection and preservation
High-quality pecan tree seed is collected, is carried out disinfection with 0.3% potassium permanganate liquid immersion treatment 3h, is used after disinfection
Clear water impregnates 5d, keeps changing water daily；
D, the cultivation of pecan tree aseptic seedling
1/2 sterilization matrix will be packed into seedlings nursing plate with 8 lines of falling root, the sterilization matrix is by weight ratio turf
Soil: vermiculite: perlite: agstone=2 ﹕, 1 ﹕, 1 ﹕ 1 is mixed, and is sterilized 2 hours at 121 DEG C after mixing, inoculation step c's
Hickory nut seed, then 2~3cm sterilization matrix covering seed is added, it sprinkles profoundly water, is placed in greenhouse and cultivates, when culture keeps matrix
Humidity 25%, 20 DEG C of greenhouse temperature, greenhouse humidity 60%；
E, it is inoculated with
After step d is cultivated 3 months, the step b microbial inoculum prepared is inoculated in seedlings nursing plate, every plant of aseptic seedling is inoculated with ferfas
Microbial inoculum 4g, then 2~3cm sterilization matrix is covered, it sprinkles profoundly water；
F, the cultivation of Arbuscular Mycorrhizal Fungi seedling
The hickory nut plant of the inoculated ferfas of step e is cultivated in greenhouse, keeps soil humidity 25%, greenhouse Air Temperature
20 DEG C of degree, greenhouse air humidity 60% obtains Panzhihua truffle mycorrhiza fungi seeding after 4~6 months.
Embodiment 2 carries out mycorrhiza fungi seeding cultivating to Panzhihua ferfas with the method for the present invention
Panzhihua truffle mycorrhiza fungi seeding cultivating, specific steps are as follows:
A, the collection and preservation of Panzhihua ferfas strain
Collect Panzhihua ferfas mature sporophore, after 75% alcohol or hydrogen peroxide dipping 5min, be ground into diameter≤
The powder of 0.5mm；
B, Panzhihua ferfas microbial inoculum with when preparation method
The ferfas powder and limestone powder, vermiculite power, turf powder, EM saccharomycete and withered grass bud that step a is prepared
Spore bacillus is matched by weight 33 ﹕, 10 ﹕, 22 ﹕, 12 ﹕, 1.5 ﹕ 1.5, is uniformly mixed；The limestone powder, vermiculite power, grass
The granularity of powdered carbon is≤0.5mm；
C, pecan tree seed collection and preservation
High-quality pecan tree seed is collected, is carried out disinfection with 0.5% potassium permanganate liquid immersion treatment 5h, is used after disinfection
Clear water impregnates 7d, keeps changing water daily；
D, the cultivation of pecan tree aseptic seedling
2/3 sterilization matrix will be packed into seedlings nursing plate with 8 lines of falling root, the sterilization matrix is by weight ratio turf
Soil: vermiculite: perlite: agstone=3 ﹕, 1 ﹕, 2 ﹕ 1.5 is mixed, and is sterilized 3 hours at 126 DEG C after mixing, inoculation step c
Hickory nut seed, then add 2~3cm sterilization matrix covering seed, sprinkle profoundly water, be placed in greenhouse and cultivate, when culture keeps base
Matter humidity 35%, 35 DEG C of greenhouse temperature, greenhouse humidity 80%；
E, it is inoculated with
After step d is cultivated 3 months, the step b microbial inoculum prepared is inoculated in seedlings nursing plate, every plant of aseptic seedling is inoculated with ferfas
Microbial inoculum 5g, then 2~3cm sterilization matrix is covered, it sprinkles profoundly water；
F, it is inoculated with the cultivation of Panzhihua truffle mycorrhiza fungi seeding
The hickory nut plant of the inoculated ferfas of step e is cultivated in greenhouse, keeps soil humidity 30%, greenhouse Air Temperature
30 DEG C of degree, greenhouse air humidity 80% obtains Panzhihua truffle mycorrhiza fungi seeding after 4~6 months.
Embodiment 3 carries out offspring cultivation to Panzhihua ferfas with the method for the present invention
Panzhihua block mycorrhiza fungi seeding cultivating, specific steps are as follows:
A, the collection and preservation of Panzhihua ferfas strain
Collect Panzhihua ferfas mature sporophore, after 75% alcohol or hydrogen peroxide dipping 5min, be ground into diameter≤
The powder of 0.5mm；
B, Panzhihua ferfas microbial inoculum with when preparation method
The ferfas powder and limestone powder, vermiculite power, turf powder, EM saccharomycete and withered grass bud that step a is prepared
Spore bacillus is matched by weight 35 ﹕, 12 ﹕, 25 ﹕, 15 ﹕, 2 ﹕ 2, is uniformly mixed；The limestone powder, vermiculite power, turf powder
Granularity be≤0.5mm；
C, pecan tree seed collection and preservation
High-quality pecan tree seed is collected, is carried out disinfection with 0.5% potassium permanganate liquid immersion treatment 3h, is used after disinfection
Clear water impregnates 5d, keeps changing water daily；
D, the cultivation of pecan tree aseptic seedling
1/2 sterilization matrix will be packed into seedlings nursing plate with 8 lines of falling root, the sterilization matrix is by weight ratio turf
Soil: vermiculite: perlite: agstone=3 ﹕, 2 ﹕, 2 ﹕ 1 is mixed, and is sterilized 3 hours at 121 DEG C after mixing, inoculation step c's
Hickory nut seed, then 2~3cm sterilization matrix covering seed is added, it sprinkles profoundly water, is placed in greenhouse and cultivates, when culture keeps matrix
Humidity 35%, 35 DEG C of greenhouse temperature, greenhouse humidity 80%；
E, it is inoculated with
After step d is cultivated 3 months, the step b microbial inoculum prepared is inoculated in seedlings nursing plate, every plant of aseptic seedling is inoculated with ferfas
Microbial inoculum 6g, then 2~3cm sterilization matrix is covered, it sprinkles profoundly water；
F, it is inoculated with the cultivation of Panzhihua truffle mycorrhiza fungi seeding
The hickory nut plant of the inoculated ferfas of step e is cultivated in greenhouse, keeps soil humidity 30%, greenhouse Air Temperature
30 DEG C of degree, greenhouse air humidity 80% obtains Panzhihua truffle mycorrhiza fungi seeding after 4~6 months.
Comparative example 1 does not add saccharomycete and bacillus subtilis carries out truffle mycorrhiza fungi seeding cultivating
In comparative example 1, in addition to not adding saccharomycete and bacillus subtilis in Panzhihua ferfas microbial inoculum proportion, remaining operation
With embodiment 1.
Comparative example 2 is not provided with the line of falling root and carries out truffle mycorrhiza fungi seeding cultivating
In comparative example 2, that in addition to the line of falling root, remaining is not operated with embodiment 1 in the seedlings nursing plate described in the step d.
Comparative example 3 carries out truffle mycorrhiza fungi seeding cultivating using existing method
Comparative example 3 is first to cultivate aseptic seedling with seedlings nursing plate using existing method, and when inoculation takes out aseptic seedling, cuts off main root,
The seedling medium that 2/3 is first placed in pot for growing seedlings, sterile bare-root seeding is put into pot for growing seedlings, is accessed ferfas microbial inoculum, is further filled with 1/3
Substrate soil.
Comparative example 4 carries out cultivating seedlings using existing method
The operating method of comparative example 4 is not in addition to accessing ferfas microbial inoculum, remaining operation is the same as comparative example 3.
Survival rate is counted when 15 days progress one-time detections, 6 months to the Va Mycorrhiza Seedling of embodiment and comparative example inoculation, is surveyed
Mycorhiza infection rate, biomass are measured, result as shown in table 1 below is obtained.
The effect table of 1 distinct methods of table progress offspring cultivation
According to the experimental results: using the method for the present invention, the truffle mycorrhiza fungi seeding formation time is short, can form ferfas bacterium within 45 days
Root, and infection rate is up to 85% or more, and nursery stock infection survival rate reaches 98% or more；The height of seedling of truffle mycorrhiza fungi seeding, ground diameter are all
Higher than existing method, root system is more and is evenly distributed, high-quality, and the quality of Va Mycorrhiza Seedling is high.
Microexamination is carried out to the mycorhiza in embodiment 1, is sampled using the methods of sampling of right-angled intersection, sampling intensity is
5%, count the synthesis situation and growing state of truffle mycorrhiza fungi.
As can be seen from the results: the pecan tree root for not being inoculated with Panzhihua ferfas is thinner, and apex is sharp (see Fig. 1)；Embodiment 1
The pecan tree root for being inoculated with Panzhihua ferfas formation mycorhiza is thicker, and form is rodlike, feather fractures, and at mycorhiza initial stage, end is in
Light yellow, color is gradually deepened in light salmon or light brown later (see Fig. 2)；
Surface can be clearly apparent under the microscope a translucent extension mycelial structure, extension mycelia is upright, not bifurcated, have
It grows to sequence at mycorhiza surface (see Fig. 3)；Mycorhiza surface layer forms the sheath cell institutional framework of the Hartig net layer of horse-hof shape (see figure
4)。
Claims (10)
1. the mycorrhiza fungi seeding cultivating method of Panzhihua ferfas, which comprises the following steps:
A, the collection and preservation of Panzhihua ferfas strain
Panzhihua ferfas mature sporophore is collected, is ground into powder after disinfection；
B, Panzhihua ferfas microbial inoculum with when preparation method
The powder that step a is prepared and agstone, vermiculite power, turf powder, EM saccharomycete and bacillus subtilis are by weight
Amount is matched than 30~35 ﹕, 8~12 ﹕, 20~25 ﹕, 10~15 ﹕, 1~2 ﹕ 1~2, is uniformly mixed；
C, pecan tree seed collection and preservation
Pecan tree seed is collected, 5~7d is impregnated after disinfection；
D, the cultivation of pecan tree aseptic seedling
Hickory nut seed after immersion is seeded into the seedlings nursing plate equipped with sterilization matrix, addition 2~3cm sterilization matrix covering kind
Son sprinkles profoundly water, and is placed in greenhouse and cultivates；
E, it is inoculated with
After the pecan tree culture of step d carries out 3 months, the step b microbial inoculum prepared is inoculated in seedlings nursing plate, every plant of nothing
Bacterination ferfas 5~8g of microbial inoculum, then 2~3cm sterilization matrix is covered, it sprinkles profoundly water；
F, the cultivation of Arbuscular Mycorrhizal Fungi seedling
The hickory nut plant of the inoculated ferfas of step e is cultivated 4~6 months in greenhouse, obtains Panzhihua truffle mycorrhiza fungi seeding.
2. the mycorrhiza fungi seeding cultivating method of Panzhihua ferfas according to claim 1, it is characterised in that: disappear described in step a
The concrete operations of poison are 3~5min of alcohol or hydrogen peroxide dipping with 70~75%.
3. the mycorrhiza fungi seeding cultivating method of Panzhihua ferfas according to claim 1, it is characterised in that: described in step a
Diameier≤0.5mm.
4. the mycorrhiza fungi seeding cultivating method of Panzhihua ferfas according to claim 1, it is characterised in that: described in step b
The granularity of limestone powder, vermiculite power and turf powder is≤0.5mm.
5. the mycorrhiza fungi seeding cultivating method of Panzhihua ferfas according to claim 1, it is characterised in that: disappear described in step c
The processing mode of poison are as follows: with 0.3~0.5% 3~5h of potassium permanganate liquid immersion treatment.
6. the mycorrhiza fungi seeding cultivating method of Panzhihua ferfas according to claim 1, it is characterised in that: go out described in step d
Bacterium matrix is by weight turfy soil: vermiculite: perlite: agstone=2~3 ﹕, 1~2 ﹕, 1~2 ﹕ 1~1.5 is mixed.
7. the mycorrhiza fungi seeding cultivating method of Panzhihua ferfas according to claim 1, it is characterised in that: go out described in step d
The sterilization process of bacterium matrix is to sterilize 2~3 hours at 121~126 DEG C.
8. the mycorrhiza fungi seeding cultivating method of Panzhihua ferfas according to claim 1, it is characterised in that: filled described in step d
The sterilization matrix entered accounts for the 1/2~2/3 of seedlings nursing plate depth.
9. the mycorrhiza fungi seeding cultivating method of Panzhihua ferfas according to claim 1, it is characterised in that: educated described in step d
4~8 lines of falling root are housed in seed plate.
10. the mycorrhiza fungi seeding cultivating method of Panzhihua ferfas according to claim 1, it is characterised in that: step d, described in e
Condition of culture are as follows: soil humidity 25~35%, 20~30 DEG C of greenhouse temperature, greenhouse humidity 60~80%.
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| CN108934768B (en) * | 2018-07-25 | 2020-11-24 | 四川省农业科学院土壤肥料研究所 | Black spore truffle inoculation microbial inoculum, treatment method thereof and mycorrhiza cultivation method |
| CN117256406A (en) * | 2023-10-23 | 2023-12-22 | 贵州省生物研究所 | Convenient artificial preparation method of fresh Lactarius deliciosus mycorrhizal seedlings |
| CN117617056B (en) * | 2024-01-25 | 2024-04-26 | 中国科学院昆明植物研究所 | Method for preparing Lijiang truffle mycorrhiza seedlings by inoculating Lijiang truffle with American hickory |
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