CN106472218A - The method of hybridization Broussonetia papyrifera non-test tube fast seedling growing - Google Patents

The method of hybridization Broussonetia papyrifera non-test tube fast seedling growing Download PDF

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Publication number
CN106472218A
CN106472218A CN201610869984.6A CN201610869984A CN106472218A CN 106472218 A CN106472218 A CN 106472218A CN 201610869984 A CN201610869984 A CN 201610869984A CN 106472218 A CN106472218 A CN 106472218A
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broussonetia papyrifera
culture
hybridization
fringe bar
parts
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覃国乐
张代江
谢琎
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Luocheng County Cunmao Biological Technology Co Ltd
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Luocheng County Cunmao Biological Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005—Cultivation methods
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00—Other inorganic fertilisers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00—Other inorganic fertilisers
    • C05D9/02—Other inorganic fertilisers containing trace elements
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity

Abstract

The invention belongs to seedling-raising technique field, especially a kind of method of hybridization Broussonetia papyrifera non-test tube fast seedling growing, soak and cuttage, accelerating germination and root culture, acclimatization and transplantses operation including fringe bar shearing, fringe bar, wherein fringe bar is soaked with cuttage is the lower cut of fringe bar to be carried out disinfection using disinfectant solution lower cut is proceeded to the yellow soil mucus containing promoting growth regulator after 20 30s and soak 1 5min, then insert in culture matrix with 35 ° of 45° angles, field planting culture；Field planting culture is to be cultivated in Small plastic shed or booth, and the time of culture is 10 40d；Culture matrix is made up of the raw material of following weight portion：10 15 parts of perlite, 38 parts of peat, 15 parts of paulownia peel, 15 parts of charcoal, 15 parts of corncob powder, 15 parts of coconut palm chaff, high temperature fiber decompose 15 parts of bacteria agent.The present invention has and greatly improves the hybridization broussonetia papyrifera seedlings speed of growth and planting benefit, low cost, emerges fast, technique is simpler, all can produce throughout the year, the advantage of suitable scale breeding.

Description

The method of hybridization Broussonetia papyrifera non-test tube fast seedling growing
Technical field
The invention belongs to seedling-raising technique field, especially a kind of method of hybridization Broussonetia papyrifera non-test tube fast seedling growing.
Background technology
Hybridization Broussonetia papyrifera, protein content and amino acid content are high, have fast-growing, high yield, multi-resistance, resistance to felling, no disease and pests harm etc. Feature, therefore also known as hybridization leaf of Broussonetia papyrifera (L.) L.Her.ex Vent..Because it has important economy and ecological value at aspects such as feedstuff, papermaking, ecological afforestations Value, has carried out a large amount of plantations in national more than 20 provinces and regions at present, wide farmers can have been helped to shake off poverty and set out on the road to prosperity, and can improve poverty The ecological environment in area, is therefore put into one of ten accurate aid-the-poor projects of China in 2015.Broussonetia papyrifera (hybridization Broussonetia papyrifera) poverty alleviation work Journey is taken the lead by office of poverty alleviation of State Council, coordinates each branch of industry, sets up " hybridization Broussonetia papyrifera industry poverty alleviation " project team, and emphasis is lean in the whole nation Hybridization leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. " woods material poultry " integration farming industry poverty alleviation is implemented in tired area.Hybridization Broussonetia papyrifera is through improvement of genes, manually A kind of fast-growing tree species with excellent resistance cultivated, can meet economy, ecological many service functions, hybridization Broussonetia papyrifera can be planted in a large number in China marginal land, can obtain crude protein Woody feed, solves the contradiction that ground is striven in agriculture and animal husbandry, helps agriculture Shake off poverty and set out on the road to prosperity in family, can improve the ecological environment of poverty-stricken area again, and under the situation of accurate poverty alleviation, Broussonetia papyrifera poverty alleviation will be lean for constructing The tired area masses shake off poverty and inject new very strong driving force.Hybridization Broussonetia papyrifera or the economy of rocky mountainous area Rocky Desertification Control and production of forestry It is worth high seeds.Hybridization Broussonetia papyrifera industry be become mountain area resource inferior position be economic advantages, ecological dominance, benefit big tor The economic industry of the area people, is that an item collection economy, society, ecological triple effect benefit the integrated agriculture industry of one.However, hybridization structure Tree nursery is the bottleneck of suppression hybridization Broussonetia papyrifera industry, and the tissue culture of hybridization Broussonetia papyrifera has Multiple Buds difficulty and seedling easily glass The technical factors such as change, tissue culture is higher to technology, equipment requirements, and fund input amount is big, and the requirement of practitioner's professional technique is high, general The problems such as logical cutting plantation is high limits its application.
At present, the growing and cultivation of hybridization Broussonetia papyrifera mainly carries out nursery using cuttage, and due to many reasons, rooting rate is relatively Low, growth cycle length is it is difficult to realize efficiently quick heavy industrialization growing and cultivation.
Content of the invention
The present invention provides a kind of method of hybridization Broussonetia papyrifera non-test tube fast seedling growing, and it can solve existing hybridization Broussonetia papyrifera cuttage Can not efficient fast breeding, thus solving the problems, such as heavy industrialization growing and cultivation.
In order to solve the above problems, the technical solution used in the present invention is：
A kind of method of hybridization Broussonetia papyrifera non-test tube fast seedling growing, soaks and cuttage, accelerating germination and life including fringe bar shearing, fringe bar Root culture, acclimatization and transplantses operation, the comprising the following steps that of the method：
S1 fringe bar is sheared：With the healthy and strong branch of hybridization Broussonetia papyrifera as material, fringe bar after shearing for the branch stays 2-3 bud Then the upper end of fringe bar is cut flat or twig stays terminal bud by point, and lower end smooths the 35 ° -45° angle in place against axillary bud point and is cut into horse ear shape, What every 20-30 branch lower end was neat is bundled into a bundle, standby；
S2 fringe bar is soaked and cuttage：The lower cut of fringe bar is carried out disinfection 20-30s using disinfectant solution, then by lower cut Proceed to containing promote growth regulator yellow soil mucus carry out soak 1-5min, afterwards with culture matrix horizontal plane be in 35 °- In 45° angle insertion culture matrix, carry out field planting culture；Described field planting culture is to be cultivated in Small plastic shed or booth, culture Time be 10-25d；
Described culture matrix is made up of the raw material of following weight portion：Perlite 10-15 part, peat 3-8 part, paulownia peel 1- 5 parts, charcoal 1-5 part, corncob powder 1-5 part, coconut palm chaff 1-5 part, high temperature fiber decompose bacteria agent 1-5 part；
S3 accelerating germination and root culture：By the fringe bar of step S2 field planting, it is 26-28 that winter controls the ambient temperature of field planting culture DEG C or accelerating germination is carried out to fringe bar by germinator, spray foliage fertilizer after young sprout, young leaves launch, promote photosynthesis, promote life Root；It is 70-90% that summer keeps the ambient humidity of field planting culture, carries out urging root culture until obtaining root system to be up to 2-8cm and life Radical is to obtain the hybridization Broussonetia papyrifera that takes root during 10-20 bar；
S4 acclimatization and transplantses：The hybridization Broussonetia papyrifera that takes root that step S3 is obtained is transplanted in seedling exercising substrate, is hidden with sunshade net Cloudy seedling exercising, the humidity of seedling exercising environment is 70-90%, can go out garden after acclimatization and transplantses 30-90d.
In the present invention, further illustrate, in S3, described germinator is made up of following raw material：Gibberellins 300- Brilliant 50-150mg, brassin lactones 100-250mg, scintilla activated water 500- are applied in 450mg, naphthalene acetic acid 100-250mg, punching 800mg, 15ml mass fraction concentration is 5% HClO4Appropriate solution.
In the present invention, further illustrate, in S3, described foliage fertilizer is made up of following raw material in parts by weight：Phosphorus Acid dihydride potassium 1-5 part, sodium selenite 1-5 part, ammonium hydrogen carbonate 1-8 part, manganese acetate 1-8 part, ferrous sulfate 1-8 part, zinc sulfate 1-8 Part, carbendazim 3-8 part and water 3-8 part.
In the present invention, further illustrate, in S4, described seedling exercising substrate is made up of following raw material in parts by weight： Garden mould 5-15 part, peat 3-8 part, cyperus iria 3-8 part, Saurauia tristyla DC. var. oldhamii (Hemsl.) Finet ＆ Gagncp. bark 3-8 part.
In the present invention, further illustrate, in S1, described hybridization Broussonetia papyrifera be give birth to then hybridization Broussonetia papyrifera stalwartness branch or The healthy and strong branch of hybridization Broussonetia papyrifera cutting seedling transplant survival.
In the present invention, further illustrate, when described hybridization Broussonetia papyrifera is the healthy and strong branch giving birth to hybridization Broussonetia papyrifera then, S2 step In described disinfectant solution be 0.1-1% potassium permanganate solution；When described hybridization Broussonetia papyrifera is the branch of cutting seedling transplant survival, S2 Described disinfectant solution in step is the dilution Bravo of 1000 times of liquid or mass fraction is the solubility Mancozeb of 75%-90% 500-1000 times of liquid of Dilution for powder.
In the present invention, further illustrate, in S3, summer field planting culture is right in the environment of booth inner sleeve Small plastic shed The fringe bar of field planting is cultivated.
In the present invention, further illustrate, in S2, described promotion growth regulator is potassium permanganate solution and IBA's Potassium salt and/or the mixed liquor of NAA, GA；The potassium salt concentration of described IBA is 50-300ppm, and NAA concentration is 1-50ppm, GA concentration For 50-100ppm；The mass concentration of described potassium permanganate solution is 0.1-1%.
In the present invention, further illustrate, the cultivation temperature in described Small plastic shed or booth is 15-25 DEG C, intensity of illumination For 1000-2000LUX, light application time is 6-8h/d, and humidity is 60-90%.
IBA of the present invention is plant growth regulator indolebutyric acid, and molecular formula is C1□H13NO2, generally use its potassium Salt or sodium salt, are a kind of auximones.
NAA of the present invention is 1-Naphthaleneacetic acid, and naphthalene acetic acid, is a kind of auximone.
GA of the present invention is gibberellins, and basic structure is gibberellane, is the class phytohormone being widely present.
Brassin lactones BR, molecular formula：C29H50O6, it is ketosteroid compouds, be one of six big phytohormone.
Culture matrix in the present invention is that paulownia peel, corncob powder, palm sugar, high temperature fiber are decomposed bacteria agent mix homogeneously After build heap ferment 18-25 days after turning process, then charcoal, perlite, peat are added into, mix.In field planting training During supporting, after processing containing the yellow soil mucus promoting growth regulator, lower cut can not only absorb the lower cut of fringe bar Growth regulator stratification, and yellow soil mucus and the hybridization cuttage growth conditionss of Broussonetia papyrifera and meet, general on the basis of this Cutting seedling field planting can be to one environment adapting to buffering of cutting seedling in culture matrix, can be by yellow soil to cutting seedling Buffer time after more preferable acclimatizing culture medium matter environment, promote to absorb the organic nutrient in culture matrix, also improve skewer The survival rate of seedlings plugging, contains the available nutrient such as substantial amounts of humic acidss and nitrogen, phosphorus, potassium, calcium, magnesium and trace element in culture matrix, And comprehensively use certain angle of inclination cuttage during cultivating, make use of action of gravity, can hestening rooting.
The germinator of the present invention is gibberellins, naphthalene acetic acid, punching to be applied crystalline substance, after brassin lactones, scintilla activated water mix Mix with standby supernatant and can get germinator.Accelerating germination agent solution is rich in a great number of elements and micronutrient solution The Germination effect of potentiation can be formed after compounding, jointly reach the purpose inspiring plant germination and growth, additional gibberellins, naphthalene second Acid, brassin lactones belong to plant growth regulator in itself, and bud can be promoted to produce itself growth hormone, using scintilla activity Water has interrupted the hydrogen bond between hydrone and has produced free water molecule, 3-6 water molecules hydrogen bond association in scintilla activated water Subset and/or single small molecule have splendid permeability, can be by each raw material for solvent using scintilla activated water Nutrient is easier to be absorbed by cutting seedling, and has splendid dissolubility, can play an active part in and improve biochemical action, strengthens enzyme Activity, promotes cutting seedling to absorb, sprouts so as to rapidly promote cutting seedling, further increase germination percentage and the seedling of cutting seedling Growth rate.
In the foliage fertilizer formulation of the present invention, potassium dihydrogen phosphate, ammonium hydrogen carbonate, sodium selenite, ammonium hydrogen carbonate, manganese acetate, sulfur Acid is ferrous, zinc sulfate provides basic n-p-k element and trace element, and potassium can adjust stomatal opening, especially to cutting seedling carbon Hydrate synthesis and transport have good facilitation；Cutting seedling vascular bundle can also be promoted to develop, and make cell wall be rich in fruit Glue material, strengthens its premunition.Ammonium hydrogen carbonate is used as nitrogenous fertilizer, can provide ammonium nitrogen and the titanium dioxide needed for growth of cuttage seedlings simultaneously Carbon, can promote growth of cuttage seedlings and photosynthesis, urge Seedling to come into leaves, simultaneously in order to prevent cutting seedling root system excessively flourishing, strain body mistake In long sprouting that is sturdy and being unfavorable for plant, and reduce the usage amount of nitrogenous fertilizer accordingly；Trace element is that plant growing institute is required A class nutrient, participate in a series of physiology and biosynthetic process in plant body, have utilization rate higher biology and Biological activity, also has the meaning of environmental protection.In sum, using the foliage fertilizer of the present invention, complement each other between raw material, no Only it is provided that suitable nutrient additionally it is possible to promote cutting seedling healthy growth during cuttage, strengthen itself disease and insect resistance Ability, improves survival rate.
Seedling exercising substrate in the present invention is to build charcoal after heap ferments 20-30 days to mix with garden mould, peat, Saurauia tristyla DC. var. oldhamii (Hemsl.) Finet ＆ Gagncp. bark Close.Comparing this stage of culture matrix belongs to the seedling exercising stage, needs the cutting seedling of field planting adapts to the ring of later stage plantation culture Border, so the material combination added will allow for the environment of plantation soil property and the nutrient substance needed for hybridization Broussonetia papyrifera, the institute in later stage With the nutrient composition content that is mixed to get above 4 kinds of raw materials for organic 5.3-8.2%, nitrogen 3.5-6.3%, phosphorus 0.21- 0.29%, potassium 6.8-8.5%, ammonium nitrogen 300-360ppm, nitrate nitrogen 5-5.8ppm, rapid available phosphorus 85-90ppm, available potassium 200- 235ppm, is compared and is directly cultivated using garden mould or peat, nutritional labeling is much not as good as the nutrient content of the formula of the present invention Half；The formula nutrient content of the present invention is specially suitable for hybridization Broussonetia papyrifera growth, mentions emergence rate.
In sum, the present invention compared with prior art has the advantages that：
1st, the speed of growth of nursery and planting benefit greatly improve：The hybridization broussonetia papyrifera seedlings cultivated using this method, 4 days start Take root, 20-30 days average rooting rates reach 90-95%, mean elements 10-20 bar, have 2 grades of root systems, garden can be gone out within 2-3 month.
2nd, the biennial seedling after cuttage root-taking, can be taken root faster with Miao Fanmiao.
3rd, can use the branch of annual growth as material introduces a collection, reduce production cost, environmental pollution is little.
4th, the process is simple of the present invention, 1 year three season all can produce, and be suitable for extensive nursery and produce.
Specific embodiment
In order that technological means, creation characteristic, reached purpose and effect that the present invention realizes are easy to understand, tie below Close embodiment, the present invention is expanded on further.It should be appreciated that specific embodiment described herein is only in order to explain the present invention, It is not intended to limit the present invention.
Embodiment 1：
A kind of method of hybridization Broussonetia papyrifera non-test tube fast seedling growing, soaks and cuttage, accelerating germination and life including fringe bar shearing, fringe bar Root culture, acclimatization and transplantses operation, the comprising the following steps that of the method：
S1 fringe bar is sheared：With the healthy and strong branch of hybridization Broussonetia papyrifera as material, fringe bar after shearing for the branch stays 2 bud points, Then the upper end of fringe bar is cut flat or twig stays terminal bud, lower end smooths the 35 ° of angles in place against axillary bud point and is cut into horse ear shape, 20 branches What lower end was neat is bundled into a bundle, standby；
S2 fringe bar is soaked and cuttage：The lower cut of fringe bar is carried out disinfection 20s using disinfectant solution, then lower cut is proceeded to Carry out soaking 1min containing the yellow soil mucus promoting growth regulator, be in 35 ° of angle insertion trainings afterwards with culture matrix horizontal plane In foster substrate, carry out field planting culture；Field planting culture is to be cultivated in Small plastic shed or booth, and the time of culture is 10d；Little arch Cultivation temperature in canopy or booth is 15 DEG C, and intensity of illumination is 1000LUX, and light application time is 6h/d, and humidity is 60%；
Wherein, culture matrix is made up of the raw material of following weight portion：10 parts of perlite, 3 parts of peat, 1 part of paulownia peel, life 1 part of thing charcoal, 1 part of corncob powder, 1 part of palm sugar, high temperature fiber decompose 1 part of bacteria agent；
Wherein, growth regulator is promoted to be potassium permanganate solution and the potassium salt of IBA and the mixed liquor of NAA, GA；Described IBA Potassium salt concentration be 50ppm, NAA concentration be 1ppm, GA concentration be 50ppm；The mass concentration of described potassium permanganate solution is 0.1%；
Wherein, hybridization Broussonetia papyrifera is the hybridization Broussonetia papyrifera stalwartness branch given birth to then；
Wherein, when hybridization Broussonetia papyrifera is the healthy and strong branch giving birth to hybridization Broussonetia papyrifera then, the described disinfectant solution in S2 step is 0.1% Potassium permanganate solution；
S3 accelerating germination and root culture：By the fringe bar of step S2 field planting, winter controls the ambient temperature of field planting culture to be 26 DEG C Or accelerating germination is carried out to fringe bar by germinator, spray foliage fertilizer after young sprout, young leaves launch, promote photosynthesis, hestening rooting； Summer is in the environment of booth inner sleeve Small plastic shed, the fringe bar of field planting to be cultivated, and the ambient humidity of holding field planting culture is 70-90%, carries out urging root culture can obtain, up to obtaining root system, the hybridization Broussonetia papyrifera that takes root up to 2cm and when taking root and counting as 10；
Wherein, germinator is made up of following raw material：Brilliant 50-150mg, Semen Brassicae Campestriss are applied in gibberellins 300mg, naphthalene acetic acid 100mg, punching Plain lactone 100-250mg, scintilla activated water 500mg, Fructus Fici or Fructus Lycopersici esculenti 3g, 12ml mass fraction concentration are 5% HClO4Appropriate solution；The preparation method of germinator is as follows：By 3g Fructus Fici or Fructus Lycopersici esculenti section, add 12ml mass fraction dense Spend the HClO for 5%4Solution, after ice bath homogenate, in ice bath, standing extracts 40min, is centrifuged with 4 DEG C, 15000 revolutions 25min, takes supernatant standby；Crystalline substance is applied in gibberellins, naphthalene acetic acid, punching, brassin lactones, scintilla activated water mix after with standby Supernatant mixing can get germinator；
Wherein, foliage fertilizer is made up of following raw material in parts by weight：1 part of potassium dihydrogen phosphate, 1 part of sodium selenite, carbonic acid 1 part of hydrogen ammonium, 1 part of manganese acetate, 1 part of ferrous sulfate, 1 part of zinc sulfate, 3 parts of carbendazim and 3 parts of water；
S4 acclimatization and transplantses：The hybridization Broussonetia papyrifera that takes root that step S3 is obtained is transplanted in seedling exercising substrate, is hidden with sunshade net Cloudy seedling exercising, the humidity of seedling exercising environment is 70%, can go out garden after acclimatization and transplantses 90d；
Wherein, seedling exercising substrate is made up of following raw material in parts by weight：5 parts of garden mould, 3 parts of peat, 3 parts of cyperus iria, 3 parts of Saurauia tristyla DC. var. oldhamii (Hemsl.) Finet ＆ Gagncp. bark.
Embodiment 2：
A kind of method of hybridization Broussonetia papyrifera non-test tube fast seedling growing, soaks and cuttage, accelerating germination and life including fringe bar shearing, fringe bar Root culture, acclimatization and transplantses operation, the comprising the following steps that of the method：
S1 fringe bar is sheared：With the healthy and strong branch of hybridization Broussonetia papyrifera as material, fringe bar after shearing for the branch stays 3 bud points, Then the upper end of fringe bar is cut flat or twig stays terminal bud, lower end smooths place's 45° angle against axillary bud point and is cut into horse ear shape, 30 branches What lower end was neat is bundled into a bundle, standby；
S2 fringe bar is soaked and cuttage：The lower cut of fringe bar is carried out disinfection 30s using disinfectant solution, then lower cut is proceeded to Carry out soaking 5min containing the yellow soil mucus promoting growth regulator, be in 45° angle insertion training afterwards with culture matrix horizontal plane In foster substrate, carry out field planting culture；Field planting culture is to be cultivated in Small plastic shed or booth, and the time of culture is 25d；Little arch Cultivation temperature in canopy or booth is 25 DEG C, and intensity of illumination is 2000LUX, and light application time is 8h/d, and humidity is 90%；
Wherein, culture matrix is made up of the raw material of following weight portion：15 parts of perlite, 8 parts of peat, 5 parts of paulownia peel, life 5 parts of thing charcoal, 5 parts of corncob powder, 1 part of palm sugar, high temperature fiber decompose 5 parts of bacteria agent；
Wherein, promote the mixed liquor that growth regulator is potassium permanganate solution and the potassium salt of IBA；The potassium salt of described IBA is dense Spend for 300ppm, the mass concentration of described potassium permanganate solution is 0.1-1%；
Wherein, hybridization Broussonetia papyrifera is the stalwartness of the hybridization Broussonetia papyrifera stalwartness branch or hybridization Broussonetia papyrifera cutting seedling transplant survival given birth to then Branch；
Wherein, when hybridization Broussonetia papyrifera is the healthy and strong branch giving birth to hybridization Broussonetia papyrifera then, the described disinfectant solution in S2 step is 1% Potassium permanganate solution；When described hybridization Broussonetia papyrifera is the branch of cutting seedling transplant survival, the described disinfectant solution in S2 step is dilution The Bravo of 1000 times of liquid；
S3 accelerating germination and root culture：By the fringe bar of step S2 field planting, it is 26-28 that winter controls the ambient temperature of field planting culture DEG C or accelerating germination is carried out to fringe bar by germinator, spray foliage fertilizer after young sprout, young leaves launch, promote photosynthesis, promote life Root；Summer is in the environment of booth inner sleeve Small plastic shed, the fringe bar of field planting to be cultivated, and keeps the ambient humidity of field planting culture For 70-90%, carry out urging root culture can obtain, up to 8cm and when taking root and counting as 20, hybridization structure of taking root up to obtaining root system Tree；
Wherein, germinator is made up of following raw material：Brilliant 50-150mg, Semen Brassicae Campestriss are applied in gibberellins 450mg, naphthalene acetic acid 250mg, punching Plain lactone 100-250mg, scintilla activated water 800mg, 10ml mass fraction concentration are 5% HClO4Appropriate solution；Germinator Preparation method as follows：The HClO that 10ml mass fraction concentration is 5%4Crystalline substance, Semen Brassicae Campestriss are applied in solution and gibberellins, naphthalene acetic acid, punching Solution mixing after plain lactone, scintilla activated water mix can get germinator；
Wherein, foliage fertilizer is made up of following raw material in parts by weight：5 parts of potassium dihydrogen phosphate, 5 parts of sodium selenite, carbonic acid 8 parts of hydrogen ammonium, 8 parts of manganese acetate, 8 parts of ferrous sulfate, 8 parts of zinc sulfate, 8 parts of carbendazim and 8 parts of water；
S4 acclimatization and transplantses：The hybridization Broussonetia papyrifera that takes root that step S3 is obtained is transplanted in seedling exercising substrate, is hidden with sunshade net Cloudy seedling exercising, the humidity of seedling exercising environment is 90%, can go out garden after acclimatization and transplantses 30d；
Wherein, seedling exercising substrate is made up of following raw material in parts by weight：15 parts of garden mould, 8 parts of peat, 8 parts of cyperus iria, 8 parts of Saurauia tristyla DC. var. oldhamii (Hemsl.) Finet ＆ Gagncp. bark.
Embodiment 3：
A kind of method of hybridization Broussonetia papyrifera non-test tube fast seedling growing, soaks and cuttage, accelerating germination and life including fringe bar shearing, fringe bar Root culture, acclimatization and transplantses operation, the comprising the following steps that of the method：
S1 fringe bar is sheared：With the healthy and strong branch of hybridization Broussonetia papyrifera as material, fringe bar after shearing for the branch stays 3 bud points, Then the upper end of fringe bar is cut flat or twig stays terminal bud, lower end smooths the 40 ° of angles in place against axillary bud point and is cut into horse ear shape, 25 branches What lower end was neat is bundled into a bundle, standby；
S2 fringe bar is soaked and cuttage：The lower cut of fringe bar is carried out disinfection 25s using disinfectant solution, then lower cut is proceeded to Carry out soaking 3min containing the yellow soil mucus promoting growth regulator, be in 40 ° of angle insertion trainings afterwards with culture matrix horizontal plane In foster substrate, carry out field planting culture；Field planting culture is to be cultivated in Small plastic shed or booth, and the time of culture is 18d；Little arch Cultivation temperature in canopy or booth is 22 DEG C, and intensity of illumination is 1500LUX, and light application time is 7h/d, and humidity is 75%；
Wherein, culture matrix is made up of the raw material of following weight portion：12 parts of perlite, 5 parts of peat, 3 parts of paulownia peel, life 3 parts of thing charcoal, 2 parts of corncob powder, 3 parts of palm sugar, high temperature fiber decompose 4 parts of bacteria agent；
Wherein, promote the mixed liquor that growth regulator is potassium permanganate solution and NAA, GA；NAA concentration is 1-50ppm, GA Concentration is 50-100ppm；The mass concentration of described potassium permanganate solution is 0.1-1%；
Wherein, hybridization Broussonetia papyrifera is the healthy and strong branch of hybridization Broussonetia papyrifera cutting seedling transplant survival；
Wherein, when hybridization Broussonetia papyrifera is the branch of cutting seedling transplant survival, the described disinfectant solution in S2 step is mass fraction Solubility 500-1000 times of liquid of Mancozeb Dilution for powder for 75%-90%；
S3 accelerating germination and root culture：By the fringe bar of step S2 field planting, it is 26-28 that winter controls the ambient temperature of field planting culture DEG C or accelerating germination is carried out to fringe bar by germinator, spray foliage fertilizer after young sprout, young leaves launch, promote photosynthesis, promote life Root；Summer is in the environment of booth inner sleeve Small plastic shed, the fringe bar of field planting to be cultivated, and keeps the ambient humidity of field planting culture For 70-90%, carry out urging root culture can obtain, up to 6cm and when taking root and counting as 15, hybridization structure of taking root up to obtaining root system Tree；
Wherein, germinator is made up of following raw material：Brilliant 50-150mg, Semen Brassicae Campestriss are applied in gibberellins 400mg, naphthalene acetic acid 200mg, punching Plain lactone 100-250mg, scintilla activated water 600mg, 15ml mass fraction concentration are 5% HClO4Appropriate solution；Germinator Preparation method as follows：The HClO that 15ml mass fraction concentration is 5%4Solution and gibberellins, naphthalene acetic acid, scintilla activated water Solution mixing after mixing can get germinator；
Wherein, foliage fertilizer is made up of following raw material in parts by weight：4 parts of potassium dihydrogen phosphate, 2 parts of sodium selenite, carbonic acid 6 parts of hydrogen ammonium, 7 parts of manganese acetate, 6 parts of ferrous sulfate, 7 parts of zinc sulfate, 6 parts of carbendazim and 6 parts of water；
S4 acclimatization and transplantses：The hybridization Broussonetia papyrifera that takes root that step S3 is obtained is transplanted in seedling exercising substrate, is hidden with sunshade net Cloudy seedling exercising, the humidity of seedling exercising environment is 80%, can go out garden after acclimatization and transplantses 55d；
Wherein, seedling exercising substrate is made up of following raw material in parts by weight：11 parts of garden mould, 7 parts of peat, 6 parts of cyperus iria, 5 parts of Saurauia tristyla DC. var. oldhamii (Hemsl.) Finet ＆ Gagncp. bark.
Embodiment 4：
A kind of method of hybridization Broussonetia papyrifera non-test tube fast seedling growing, concrete steps are same as Example 3, and difference is culture medium Matter is made up of peat, and seedling exercising substrate is made up of garden mould.
Embodiment 5：
A kind of method of hybridization Broussonetia papyrifera non-test tube fast seedling growing, concrete steps are same as Example 3, and difference foliage fertilizer makes Entered high-efficiency foliage fertilizer with good fortune.
Embodiment 6：
A kind of method of hybridization Broussonetia papyrifera non-test tube fast seedling growing, concrete steps are same as Example 3, and difference germinator makes With Shandong fail in an entrance examination biological pesticide company limited production plentiful god dew No. 1.
The garden time that goes out of the hybridization Broussonetia papyrifera that embodiment 1-6 kind is planted is recorded, right behind 10 days after using germinator Bud ratio carries out counting, after 7 days after carrying out Foliar Spraying, the situation of taking root is recorded, and implements each to after seedling exercising Example 1-6 takes 10 plants of plant field planting in the big Tanaka of soil property identical, is colonized 30 days statistics survival rates and is recorded；Concrete condition is shown in Table 1：
Table 1 hybridizes Broussonetia papyrifera growing state
Project Go out the garden time/sky Bud ratio/% Root length/cm Take root number/cm Survival rate/%
Embodiment 1 90 95 2 10 95
Embodiment 2 30 98 8 20 97
Embodiment 3 55 97 6 15 96
Embodiment 4 82 95 5 13 87
Embodiment 5 62 95 3 7 89
Embodiment 6 61 90 5 12 89
As seen from the above table, using technical scheme, go out the garden time at 1-3 month, bud ratio all reach 95% with On, number of taking root all reaches more than 10cm, and survival rate all reaches more than 95%；Compare embodiment 4, not using the culture of the application Substrate and seedling exercising substrate are cultivated, and the survival rate in field planting land for growing field crops will at least reduce by 8% point；Compare embodiment 5, do not adopt The foliage fertilizer of the present invention, number of taking root compares the same period will short and quantity minimizing relatively；Compare embodiment 6, do not invented using basic Germinator, the bud ratio comparing the cutting seedling of the same period compares and reduces at least five percentage points.

Claims (9)

1. a kind of method of hybridization Broussonetia papyrifera non-test tube fast seedling growing, includes fringe bar shearing, fringe bar immersion and cuttage, accelerating germination and take root Culture, acclimatization and transplantses operation are it is characterised in that the comprising the following steps that of the method：
S1 fringe bar is sheared：With the healthy and strong branch of hybridization Broussonetia papyrifera as material, fringe bar after shearing for the branch stays 2-3 bud point, so Afterwards the upper end of fringe bar is cut flat or twig stays terminal bud, lower end smooths the 35 ° -45° angle in place against axillary bud point and is cut into horse ear shape, 20-30 What individual branch lower end was neat is bundled into a bundle, standby；
S2 fringe bar is soaked and cuttage：The lower cut of fringe bar is carried out disinfection 20-30s using disinfectant solution, then lower cut is proceeded to Carry out soaking 1-5min containing the yellow soil mucus promoting growth regulator, then, in 35 ° -45° angle insertion culture matrix, to enter Row field planting culture；Described field planting culture is to be cultivated in Small plastic shed or booth, and the time of culture is 10-25d；
Described culture matrix is made up of the raw material of following weight portion：Perlite 10-15 part, peat 3-8 part, paulownia peel 1-5 part, Charcoal 1-5 part, corncob powder 1-5 part, coconut palm chaff 1-5 part, high temperature fiber decompose bacteria agent 1-5 part；
S3 accelerating germination and root culture：By the fringe bar of step S2 field planting, it is 16-20 that winter controls the canopy temperature of field planting culture DEG C, using germinator, accelerating germination is carried out to fringe bar, spray foliage fertilizer after young sprout, young leaves launch, promote photosynthesis, promote life Root；It is 70-90% that summer keeps the ambient humidity of field planting culture, carries out urging root culture until obtaining root system to be up to 2-8cm and life Radical is to obtain regeneration hybridization broussonetia papyrifera seedlings during 10-20 bar；
S4 transplants：The hybridization broussonetia papyrifera seedlings of taking root that step S3 is obtained are transplanted in seedling exercising substrate, and carry out sheltering from heat or light seedling exercising with sunshade net, The humidity of seedling exercising environment is 70-90%, can go out garden after acclimatization and transplantses 30-90d.
2. the method for hybridization Broussonetia papyrifera non-test tube fast seedling growing as claimed in claim 1 is it is characterised in that in S3, described accelerating germination Agent is made up of following raw material：Brilliant 50-150mg, brassin lactones are applied in gibberellins 300-450mg, naphthalene acetic acid 100-250mg, punching 100-250mg, scintilla activated water 500-800mg, mass fraction concentration are 5% HClO4Appropriate solution.
3. the method for hybridization Broussonetia papyrifera non-test tube fast seedling growing as claimed in claim 1 is it is characterised in that in S3, described blade face Fertilizer is made up of following raw material in parts by weight：Potassium dihydrogen phosphate 1-5 part, sodium selenite 1-5 part, ammonium hydrogen carbonate 1-8 part, acetic acid Manganese 1-8 part, ferrous sulfate 1-8 part, zinc sulfate 1-8 part, carbendazim 3-8 part and water 3-8 part.
4. the method for hybridization Broussonetia papyrifera non-test tube fast seedling growing as claimed in claim 1 is it is characterised in that in S4, described seedling exercising Substrate is made up of following raw material in parts by weight：Garden mould 5-15 part, peat 3-8 part, cyperus iria 3-8 part, Saurauia tristyla DC. var. oldhamii (Hemsl.) Finet ＆ Gagncp. bark 3-8 part.
5. the method for hybridization Broussonetia papyrifera non-test tube fast seedling growing as claimed in claim 1 is it is characterised in that in S1, described hybridization Broussonetia papyrifera is the healthy and strong branch of the hybridization Broussonetia papyrifera stalwartness branch or hybridization Broussonetia papyrifera cutting seedling transplant survival given birth to then.
6. the method for hybridization Broussonetia papyrifera non-test tube fast seedling growing as claimed in claim 5 is it is characterised in that described hybridization Broussonetia papyrifera is to work as During the healthy and strong branch of year raw hybridization Broussonetia papyrifera, the described disinfectant solution in S2 step is the potassium permanganate solution of 0.1-1%；Described hybridization When Broussonetia papyrifera is the branch of cutting seedling transplant survival, the described disinfectant solution in S2 step is Bravo or the quality of 1000 times of liquid of dilution Fraction is solubility 500-1000 times of liquid of Mancozeb Dilution for powder of 75%-90%.
7. the method for hybridization Broussonetia papyrifera non-test tube fast seedling growing as claimed in claim 1 is it is characterised in that in S3, summer is colonized Culture is in the environment of booth inner sleeve Small plastic shed, the fringe bar of field planting to be cultivated.
8. the method for hybridization Broussonetia papyrifera non-test tube fast seedling growing as claimed in claim 1 is it is characterised in that in S2, described promotion Growth regulator is potassium permanganate solution and the potassium salt of IBA and/or the mixed liquor of NAA, GA；The potassium salt concentration of described IBA is 50- 300ppm, NAA concentration is 1-50ppm, and GA concentration is 50-100ppm；The concentration of described potassium permanganate solution is 0.1-1%.
9. the method for hybridization Broussonetia papyrifera non-test tube fast seedling growing as claimed in claim 1 is it is characterised in that described Small plastic shed or booth Interior cultivation temperature is 15-25 DEG C, and intensity of illumination is 1000-2000LUX, and light application time is 6-8h/d, and humidity is 60-90%.
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CN107129365A (en) * 2017-05-18 2017-09-05 中科天华生物科技有限公司 A kind of culture medium for paper mulberry nursery
CN107372111A (en) * 2017-08-03 2017-11-24 湖北理工学院 A kind of celery artificial seed and its fast preparation method
CN109429750A (en) * 2017-08-31 2019-03-08 王子成 A kind of hybridization paper mulberry four seasons cuttage and quick-propagation method for culturing seedlings
CN111527901A (en) * 2020-06-23 2020-08-14 贵港市农业科学研究院 Passion fruit cuttage stock grafting seedling method
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CN107094565A (en) * 2017-04-01 2017-08-29 阜南县鑫荣种养专业合作社 A kind of North-Anhui district pear tree lists method in advance
CN107098756A (en) * 2017-05-10 2017-08-29 麦丽 A kind of cuttage and seedling culture method of paper mulberry
CN107129365A (en) * 2017-05-18 2017-09-05 中科天华生物科技有限公司 A kind of culture medium for paper mulberry nursery
CN107372111A (en) * 2017-08-03 2017-11-24 湖北理工学院 A kind of celery artificial seed and its fast preparation method
CN107372111B (en) * 2017-08-03 2019-07-23 湖北理工学院 A kind of celery artificial seed and its fast preparation method
CN109429750A (en) * 2017-08-31 2019-03-08 王子成 A kind of hybridization paper mulberry four seasons cuttage and quick-propagation method for culturing seedlings
CN111527901A (en) * 2020-06-23 2020-08-14 贵港市农业科学研究院 Passion fruit cuttage stock grafting seedling method
CN115399215A (en) * 2022-10-11 2022-11-29 贵州务川科华生物科技有限公司 Preparation method of soil matrix for paper mulberry seedling hardening period

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