CN115349580A - Breeding substrate and artificial breeding method for cyenopsis griseus - Google Patents
Breeding substrate and artificial breeding method for cyenopsis griseus Download PDFInfo
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- 238000009395 breeding Methods 0.000 title claims abstract description 92
- 230000001488 breeding effect Effects 0.000 title claims abstract description 84
- 239000000758 substrate Substances 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 claims abstract description 40
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 29
- 235000009566 rice Nutrition 0.000 claims abstract description 29
- 239000002994 raw material Substances 0.000 claims abstract description 12
- 241000895811 Myza Species 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 95
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- 239000007788 liquid Substances 0.000 claims description 35
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- 239000011358 absorbing material Substances 0.000 claims description 2
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
A raising substrate for cyenopsis griseus and an artificial raising method are disclosed, wherein the raising substrate for cyenopsis griseus is prepared by taking rice as a main raw material; the invention also comprises an artificial feeding method of cyenopsis griseus by using the feeding substrate. The breeding substrate for the myza griseola hayata is low in cost, easy to obtain and good in breeding effect; the method is simple, and can be used for indoor mass breeding of the cyenopsis griseus so as to pollinate crops in facility agriculture and fields.
Description
Technical Field
The invention relates to a substrate and a method for feeding myzus persicae, in particular to a substrate and a method for feeding myzus persicae with cyrtomyzus persicae.
Background
Aphid-eating flies can be classified into species of predatory nature, saprophagic nature, phytophagous nature and the like according to the feeding habits of larvae. The larvae of predatory aphid flies can prey on aphids, scale insects, plant hoppers and other pests, and are important natural enemy insects. The larva of the saprophagous aphidius gifuensis eats organic matters such as rotten animal and plant corpses, animal wastes and the like, and is a decomposer of an ecological system. The larvae of the phytophagous aphid flies eat the tissues of roots, stems, leaves and the like of plants, and part of the pests are pests.
Myza griseola (A. Nivea)Eristalis cerealis) (ii) genus Pirophagidae (Syrphidae) of the order Diptera (Diptera)Eristslis lathelle, 1804), a saprophagic aphid fly; the individual development of the gray zone aphid fly is divided into 4 insect states of egg, larva, pupa and imago, wherein the larva stage can be divided into 1-instar larva, 2-instar larva and 3-instar larva. The larva takes putrefactive organic matters or livestock feces as food, and adults take pollen and nectar, which are important pollination insects in the nature.
In recent years, artificial feeding research on predatory aphid flies, such as wide-band superior aphid flies, black-banded aphid flies and the like, is more. Most adults of predatory aphid flies and saprophytic aphid flies have the habit of visiting flowers, and the larvae are different; the larvae of predatory aphid flies are nutritious by sucking the juice of aphids through a sucking mouth device, and the mouth device of the saprophagous aphid fly larvae is different from the predatory aphid fly larvae and cannot prey on the aphids. At present, the artificial feeding of saprophagous aphidius gifuensis is hardly researched.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide a breeding substrate and an artificial breeding method for cyenopsis griseus, which can realize mass propagation of cyenopsis griseus.
The technical scheme adopted by the invention for solving the technical problems is as follows: a raising substrate for Siphonius griseus is characterized by being prepared from rice serving as a main raw material.
Preferably, the preparation method comprises the following steps: and putting the cooled undercooked rice and cold water in a container, enabling the water surface to submerge the rice, sealing, and storing for more than two days at the temperature of 20-30 ℃ to obtain the finished product. After the paddy is soaked in water and sealed for storage, the water liquid on the surface is clear and has a faint scent. The breeding substrate for Siphonius griseus comprises treated grains (i.e. solid matter) and a small amount of clear liquid. The undercooked rice is obtained by cooking or steaming.
Preferably, the rice is boiled in a pot with cold water until the water is boiled, and the boiling is continued for 3 to 5 minutes to obtain the undercooked rice; the husk of the undercooked rice is complete.
The artificial breeding method of cyenopsis griseus by using the breeding substrate is characterized by comprising the following steps:
the method for obtaining the eggs comprises the following steps: placing adult female and male gray pipe myzus persicae in an insect breeding cage, and feeding the adult myzus persicae with ground pollen, 10-20wt% of honey water, 8-15wt% of cane sugar water and clear water; the honey water, the cane sugar water and the clear water are respectively soaked in the porous water absorption material, and no clear water exists in the insect cage; the insect breeding cage is internally provided with an insect breeding box A filled with breeding substrate and water, the liquid level height in the insect breeding box A is 0.8 to 1.5cm lower than the surface of the solid matter, and water is added in time when the liquid level height is too low; after the myza virgata with the ash belt tube lays eggs in the insect breeding box A, covering the insect breeding box A, and reserving the air holes to wait for incubation; the insect-raising cage can be made of air-permeable materials, and can also be purchased with products sold on the market; when the liquid level in the insect box A is too high or open water exists in the insect cage, adults are easy to die;
the larva breeding method comprises the following steps: after the larvae are hatched, increasing the liquid level height in the larva breeding box A for breeding; transferring the larva to an insect box B filled with a feeding substrate and water when the length of the larva is 0.4-0.6 cm, wherein the liquid level in the insect box B is higher than the surface of the solid matter; when the liquid level in the insect breeding box B is too low, the breeding fails;
the collection method of pupae comprises the following steps: placing the insect breeding box B above a plane which is paved with sand and has an area larger than the bottom surface of the insect breeding box B, enabling the larva to climb out of the insect breeding box B, enter the sand to pupate, and collecting the pupae from the sand; enabling the myxomyza griseovii larvae to pupate after 3 years of age, and timely placing the insect breeding box B above sand according to the growth condition of the larvae;
the eclosion method of the pupae comprises the following steps: storing the pupae under the condition of humidity of 50 to 60 percent, and obtaining imagoes after the pupae are eclosized; the preservation environment is to ensure that no clear water exists.
Preferably, the thickness of the solid matter in the insect box A is 2-4 cm.
Preferably, after the larvae are hatched, water is added so that the difference between the liquid level in the insect-rearing box A and the solid matter surface is less than 0.2cm.
Preferably, the thickness of the solid matter in the insect box B is 0.8 to 2cm.
Preferably, the liquid level in the insect breeding box B is 0.5 to 1.5cm higher than the surface of the solid matter.
Preferably every 30 dm 3 The space of the insect cage is used for containing ash to bring about 80 to 130 heads of adult aphid flies, the male-female ratio is 0.9 to 1.1, and the area of the bottom surface of a corresponding insect box A is 25 to 35cm 2 。
Preferably, every 100cm in the insect-breeding box B 2 The bottom surface area of the artificial pond is 70 to 90 heads of larvae.
Preferably, the particle size of the ground pollen is less than 100 mesh.
Preferably, the porous water absorbing material is one or more of cotton balls, non-woven fabrics and sponges.
Preferably, the thickness of the sand is 0.8 to 1.5cm.
Preferably, the sand has a particle size of less than 1.5mm.
Preferably, the water content of the sand is 5 to 10wt%.
Preferably, the temperature in the feeding process is 16-32 ℃.
After the imagoes are obtained by the method, the method can be recycled to obtain a large number of imagoes with myzus griseus.
The substrate for breeding the cyromorus griseus is a most suitable substrate for breeding the cyromorus griseus, which is found by an inventor from various breeding substrates, has a good breeding effect, can be used from an egg stage to a whole larva stage, does not need to provide nutrients except water during use, and obviously reduces the breeding difficulty; if necessary, the additive can be additionally supplemented on the basis of the invention, thus achieving the effects of prolonging the storage time, preventing diseases and the like.
The invention has the beneficial effects that:
(1) The feeding substrate for the myza griseola linnaeus is low in cost, easy to obtain and good in feeding effect;
(2) The method is simple, and can be used for indoor mass breeding of the myzus persicae in the grey zone so as to pollinate crops in facility agriculture and fields.
Detailed Description
The present invention is further illustrated by the following examples.
The starting materials used in the examples of the present invention were all obtained from conventional commercial sources.
Example 1
The substrate for raising cyenopsis griseus of the embodiment is prepared by taking rice as a main raw material. Firstly, washing the paddy, putting the paddy into a pot, boiling the paddy with water, and boiling the paddy for 4 minutes; pouring the boiled paddy into a container, washing with cold water to normal temperature, adding water into the container to submerge the paddy, covering a cover for sealing, placing at 25 ℃, and storing for two days to obtain the feeding substrate. The feeder substrate for myzus griseus comprises treated grains (i.e. solid matter) and a small amount of clear liquid.
The temperature of the whole feeding process of the embodiment is 24 +/-1 ℃, and the artificial feeding method of the cyromorus griseus comprises the following steps:
the method for obtaining the eggs comprises the following steps: preparing an insect cage with the aperture of 30cm multiplied by 30cm by using gauze with the aperture of 1mm multiplied by 1mm, putting 50 heads of adult female and male ashen tubificidae flies (100 heads in total) in the insect cage, and feeding the adult with crushed pollen (screened by a 100-mesh sieve), 15wt% of honey water, 10wt% of cane sugar water and clean water; the honey water, the cane sugar water and the clear water are respectively soaked in the cotton balls, and no clear water exists in the insect cage; a circular insect culturing box A which is 7cm high, 3cm in radius and is filled with a culturing substrate and water is arranged in the insect culturing cage, the thickness of a solid substance in the insect culturing box A is 3cm, the liquid level height is 1cm lower than the surface of the solid substance (namely the liquid level height is 2 cm), and water is added in time when the liquid level height is reduced; after the myzus persicae with the ash belt lay eggs in the insect breeding box A, covering the insect breeding box A (the cover is provided with air holes), and waiting for hatching;
the larva breeding method comprises the following steps: after the larvae are hatched, the liquid level in the larva breeding box A is added to be level with the solid matter for breeding; when the larva grows to be 0.4-0.6 cm in length, the larva is transferred to the area of the bottom surface of 348.75 cm 2 The thickness of solid matters in the square insect box B is 1cm, and the liquid level is 0.5cm higher than the surface of the solid matters;
the collection method of pupae comprises the following steps: placing the insect breeding box B above a tray which is flatly paved with sand with the thickness of 1cm and has the area larger than the bottom surface of the insect breeding box B, enabling the larva to climb out of the insect breeding box B, enter the sand to pupate, and collecting the pupae from the sand; the grain size of the sand is less than 1.5mm, and the water content of the sand is 5wt%;
the eclosion method of the pupae comprises the following steps: storing the pupae under the condition that the humidity is 50-60%, and obtaining imagoes after the pupae eclosion; the preservation environment is to ensure that no clear water exists.
The breeding of the myzus griseus in this example is shown in table 1.
TABLE 1 Breeding of Siphonella griseola (24. + -. 1 ℃ C.)
| Preosteogenesis/d | 16.3±0.7d |
| Oviposition period/d | 4±0.3d |
| Oviposition amount/granule | 100 to 150 granules |
| Egg period/d | 1.9±0.5d |
| Hatching rate/% | 95±0.5% |
| Larval stage/d | 19.3±0.4d |
| Pupation Rate/% | 81±0.14% |
| Pupal stage/d | 7.2±0.4d |
| Eclosion Rate/% | 90±0.2% |
Example 2
The substrate for raising cyenophora griseofulvus of the embodiment is prepared by taking rice as a main raw material. Firstly, washing rice, putting the rice into a pot, and adding water to steam for 2 minutes; pouring the steamed paddy into a container, cooling to normal temperature, adding water into the container to submerge the paddy, covering the container with a cover, placing the container at 26 ℃, and preserving for three days to obtain the breeding substrate. The feeder substrate for myzus griseus comprises treated grains (i.e. solid matter) and a small amount of clear liquid.
The temperature of the whole feeding process of the embodiment is 20 +/-1 ℃, and the artificial feeding method of the cyromorus griseus comprises the following steps:
the method for obtaining the eggs comprises the following steps: the using volume is 50dm 3 The square insect-raising cage is characterized in that 75 heads of adult female and male aphid flies (150 heads in total) are placed in the insect-raising cage, and the adult flies are fed with crushed pollen (screened by a 100-mesh sieve), 10wt% of honey water, 15wt% of cane sugar water and clear water; the honey water, the cane sugar water and the clear water are respectively soaked in the non-woven fabric pieces, and no clear water exists in the insect cage; the bottom surface area of the insect breeding cage is 50 cm 2 The device comprises a square insect breeding box A filled with breeding substrates and water, wherein the thickness of solid matters in the insect breeding box A is 2.5cm, the liquid level height is 1.5cm lower than the surface of the solid matters (namely the liquid level height is 1 cm), and water is added in time when the liquid level height is reduced; after the myzus persicae with the ash belt lay eggs in the insect breeding box A, covering the insect breeding box A (the cover is provided with air holes), and waiting for hatching;
the larva breeding method comprises the following steps: after the larvae are hatched, the liquid level in the larva breeding box A is added to be level with the solid matter for breeding; when the larva grows to 0.4-0.6 cm in length, the larva is transferred to the bottom surface with the area of 200.96 cm 2 In an insect box B (putting 150 to 180 larvae in each box) filled with a feeding substrate and water, the thickness of a solid matter in the insect box B is 1.5cm, and the liquid level is 1.5cm higher than the surface of the solid matter;
the collection method of pupae comprises the following steps: placing the insect breeding box B above a table top paved with sand with the thickness of 1.5cm and the area of the table top larger than the bottom surface of the insect breeding box B, enabling the larva to climb out of the insect breeding box B, enter the sand to pupate, and collecting the pupae from the sand; the grain size of the sand is less than 1.5mm, and the water content of the sand is 10wt%;
the eclosion method of the pupae comprises the following steps: storing the pupae under the condition that the humidity is 50-60%, and obtaining imagoes after the pupae eclosion; the preservation environment is to ensure that no clear water exists.
The breeding of the myzus ashmead in this example is shown in table 2.
TABLE 2 Breeding of cyenopsis avenae (20. + -. 1 ℃ C.)
| Pre-spawning period/d | 18.5±0.7d |
| Oviposition period/d | 5.0±0.3d |
| Egg laying amount/granule | 90 to 110 granules |
| Egg period/d | 4.3±0.2d |
| Hatching rate/% | 90±0.15% |
| Larval stage/d | 22±1.2d |
| Pupation Rate/% | 77.18±0.02% |
| Pupal stage/d | 11.3±0.8d |
| Eclosion Rate/% | 82.05±0.13% |
Example 3
The substrate for raising cyenopsis griseus of the embodiment is prepared by taking rice as a main raw material. Firstly, washing rice, putting the rice into a pot, boiling the rice with water, and boiling the rice for 5 minutes; pouring the cooked paddy into a container, washing with cold water to normal temperature, adding water into the container to submerge the paddy, sealing, placing at 27 ℃, and storing for two days to obtain the feeding substrate. The feeder substrate for myzus griseus comprises treated grains (i.e. solid matter) and a small amount of clear liquid.
The temperature of the whole feeding process of the embodiment is 30 +/-1 ℃, and the artificial feeding method of the cyromorus griseus comprises the following steps:
the method for obtaining the eggs comprises the following steps: the use volume is 20dm 3 The square insect-raising cage is characterized in that 40 heads (80 heads in total) of adult female and male aphid flies with ash pipes are placed in the insect-raising cage, and the adult flies are fed with crushed pollen (screened by a 100-mesh sieve), 20wt% of honey water, 8wt% of cane sugar water and clear water; the honey water, the cane sugar water and the clear water are respectively soaked in the sponge pieces, and no clear water exists in the insect cage; the bottom surface area of the insect breeding cage is 18.84 cm 2 The device comprises an elliptical insect breeding box A filled with breeding substrates and water, wherein the thickness of solid matters in the insect breeding box A is 4cm, the liquid level height is 0.8cm lower than the surface of the solid matters (namely the liquid level height is 3.2 cm), and water is added in time when the liquid level height is reduced; after the myzus persicae with the ash belt lay eggs in the insect breeding box A, covering the insect breeding box A (the cover is provided with air holes), and waiting for hatching;
the larva breeding method comprises the following steps: after the larvae are hatched, the liquid level in the larva breeding box A is added to be level with the solid matter for breeding; when the larva grows to be 0.4 to 0.6cm in length, the larva is transferred to the bottom surface with the area of 500 cm 2 The solid matter in the insect breeding box B is 2cm thick and the liquid level is 1.2cm higher than the surface of the solid matter in the insect breeding box B (every box is used for breeding 350 to 400 larvae);
the collection method of pupae comprises the following steps: placing the insect breeding box B above a platform which is paved with sand with the thickness of 0.8cm and has the area larger than the bottom surface of the insect breeding box B, enabling the larva to climb out of the insect breeding box B, enter the sand to pupate, and collecting the pupae from the sand; the grain size of the sand is less than 1.5mm, and the water content of the sand is 5wt%;
the eclosion method of the pupae comprises the following steps: storing the pupae under the condition that the humidity is 50-60%, and obtaining imagoes after the pupae eclosion; the preservation environment is to ensure that no clear water exists.
The breeding of the myzus griseus in this example is shown in table 3.
Table 3 Breeding conditions of Siberian avenae (30 +/-1 ℃ C.)
| Pre-spawning period/d | 9.3±0.7d |
| Oviposition period/d | 3.8±0.6d |
| Egg laying amount/granule | 70 to 110 granules |
| Egg stage/d | 1.5±0.3d |
| Hatching rate/% | 89.56±0.3d |
| Larval stage/d | 14±0.3d |
| Pupation Rate/% | 81.33±0.3% |
| Pupal stage/d | 5.3±0.6d |
| Feathering rate/% | 80±0.6% |
Example 4
In the comparative example, rice was used as a main raw material to prepare a substrate for raising myza griseofulvin, and the cooking time of rice was prolonged as compared with that in example 1. The preparation method comprises the following steps: washing paddy, putting the paddy into a pot, boiling the paddy with water, and boiling the paddy for 7 minutes; pouring the boiled paddy into a container, washing with cold water to normal temperature, adding water into the container to submerge the paddy, covering a cover for sealing, placing at 25 ℃, and storing for two days.
The same artificial feeding method as that in example 1 was used to feed cyenopsis griseus, and the breeding conditions of cyenopsis griseus were studied. The results are shown in Table 4.
TABLE 4 Breeding of Siphonella griseola (24. + -. 1 ℃ C.)
| Pre-spawning period/d | 15.8±0.4d |
| Oviposition period/d | 3.8±0.5d |
| Oviposition amount/granule | 76 to 98 grains |
| Egg stage/d | 1.8±0.3d |
| Hatching rate/% | 90±0.2% |
| Larval stage/d | 17.2±0.33d |
| Pupation Rate/% | 69±0.28% |
| Pupal stage/d | 6.9±0.4d |
| Feathering rate/% | 76.5±0.2% |
When the cooking time of the rice is too long, part of the rice grains are popped. On the prepared feed substrate, the egg laying amount, pupation rate and emergence rate of the cyenopsis griseus are reduced compared with those of the feed substrate prepared in the example 1.
Comparative example
(1) Highland barley, sorghum and pearl barley are respectively used as main raw materials to prepare the feed matrix for artificially feeding cyenopsis griseus. Sorghum and coix seeds are relatively harder to cook, highland barley is relatively easier to cook, and the cooking time is properly prolonged or shortened when the corresponding feed matrix is prepared, so as to achieve the same degree of cooking.
The preparation method of the feed matrix taking highland barley as a main raw material comprises the following steps: washing semen Avenae Nudae, decocting in water for 2 min after boiling; pouring the cooked semen Avenae Nudae into a container, washing with cold water to normal temperature, adding water into the container to submerge the semen Avenae Nudae, sealing, and storing at 25 deg.C for two days.
The preparation method of the feed matrix taking sorghum as a main raw material comprises the following steps: washing sorghum, putting into a pot, boiling with water, and boiling for 3 minutes; pouring the cooked sorghum into a container, washing with cold water to normal temperature, adding water into the container to submerge the sorghum, sealing, and storing at 25 ℃ for two days.
The preparation method of the feed matrix taking the coix seeds as the main raw material comprises the following steps: washing Coicis semen with shell, decocting in water for 5 min after boiling; pouring the cooked Coicis semen into a container, washing with cold water to normal temperature, adding water into the container to submerge Coicis semen, sealing, and storing at 25 deg.C for two days.
(2) The same artificial feeding method as in example 1 was used to feed cyenopsis griseus using feed substrates mainly comprising highland barley, sorghum, and coix seeds, respectively, and the breeding conditions of cyenopsis griseus were studied. The results are shown in Table 5.
Table 5 Breeding of Siberian avenae flies using different feed substrates (24 + -1 deg.C)
| Main raw material of feed matrix | Highland barley | (sorghum) | Coix seed |
| Pre-spawning period/d | 15.8±0.2d | 15.3±0.7d | 17.2±0.33d |
| Oviposition period/d | 4±0.12d | 3.5±0.3d | 4±0.51d |
| Egg laying amount/granule | 89 to 106 grains | 98-131 granules | 75 to 124 granules |
| Egg period/d | 1.7±0.2d | 1.9±0.5d | 1.8±0.3d |
| Hatching rate/% | 90±0.3% | 93±0.5% | 91±0.2% |
| Larval stage/d | 21.3±0.3d | 23.3±0.14d | 24.3±0.6d |
| Pupation Rate/% | 61±0.27% | 73±0.14% | 75±0.38% |
| Pupal stage/d | 8.9±0.1d | 8.4±0.4d | 9.8±0.3d |
| Eclosion Rate/% | 51.5±0.28% | 81.5±0.2% | 74.3±0.4% |
It can be seen from table 5 and table 1 that, on the feed substrate prepared from rice, the laying amount of mythimna griseola is the largest, the hatching rate, the pupation rate and the eclosion rate are the highest, and the larval stage and the pupation stage are the shortest. The artificial feeding work of the early oviposition stage and the oviposition stage of the cycromyza griseiformis is to feed adults, and the adults do not eat a feeding substrate, so that the change of the feeding substrate has little influence on data of the early oviposition stage and the oviposition stage.
Claims (10)
1. A raising substrate for cyenophorus griseus is characterized by being prepared from rice serving as a main raw material.
2. The raising substrate for cyenopsis griseus according to claim 1, wherein the preparation method comprises the steps of: putting the cooled undercooked rice and cold water in a container, leading the water surface to submerge the rice, sealing, and storing for more than two days at the temperature of 20-30 ℃ to obtain the finished product; the breeding substrate of the myzus grey-banded aphids comprises solid matters and clear liquid; the undercooked rice is obtained by cooking or steaming.
3. The raising substrate for Aphis griseofulvus according to claim 2, wherein rice is boiled in a pot with cold water until the water is boiled, and the boiling is continued for 3 to 5 minutes to obtain undercooked rice; the husk of the undercooked rice is complete.
4. An artificial feeding method of cyenopsis griseus using the feeding substrate of any one of claims 1 to 3, characterized in that:
the method for obtaining the eggs comprises the following steps: placing adult female and male gray pipe myzus persicae in an insect breeding cage, and feeding the adult myzus persicae with ground pollen, 10-20wt% of honey water, 8-15wt% of cane sugar water and clear water; the honey water, the cane sugar water and the clear water are respectively soaked in the porous water absorption material, and no clear water exists in the insect cage; the insect breeding cage is internally provided with an insect breeding box A filled with breeding substrates and water, the liquid level height in the insect breeding box A is 0.8 to 1.5cm lower than the surface of the solid matter, and water is added in time when the liquid level height is too low; after the myza virgata with the ash belt tube lays eggs in the insect breeding box A, covering the insect breeding box A, and reserving the air holes to wait for incubation;
the larva breeding method comprises the following steps: after the larvae are hatched, increasing the liquid level in the larva breeding box A, and breeding; when the larva grows to be 0.4 to 0.6cm in length, transferring the larva to an insect box B filled with a feeding substrate and water, wherein the liquid level in the insect box B is higher than the surface of a solid matter;
the collection method of pupae comprises the following steps: placing the insect culturing box B above a plane which is paved with sand and has an area larger than the bottom surface of the insect culturing box B, allowing the larva to climb out of the insect culturing box B, enter the sand to pupate, and collecting pupae from the sand;
the eclosion method of the pupae comprises the following steps: storing the pupae under the condition that the humidity is 50-60%, and obtaining imagoes after the pupae eclosion; the preservation environment is to ensure that no clear water exists.
5. The method for artificially feeding cyromorus griseus according to claim 4, wherein the thickness of the solid matter in the insect box A is 2-4 cm; after the larvae are hatched, adding water to ensure that the height difference between the liquid level in the larva breeding box A and the surface of the solid matter is less than 0.2cm; the thickness of the solid matter in the insect box B is 0.8 to 2cm; the liquid level in the insect box B is 0.5 to 1.5cm higher than the surface of the solid matter.
6. The method for artificially feeding myza griseola according to claim 4 or 5, wherein the amount is 30 dm per day 3 The space of the insect cage is used for containing the adult myzus persicae with the ash carrying pipe, the ratio of male to female is 0.9 to 1.1, and the area of the bottom surface of a corresponding insect box A is 25 to 35cm 2 (ii) a In the insect-culturing box B, every 100cm 2 The bottom surface area of the artificial pond is 70 to 90 heads of larvae.
7. The method for artificially feeding myzus grey-belt pipe aphid flies according to any one of claims 4 to 6, wherein the particle size of the ground pollen is less than 100 meshes.
8. The method for artificially feeding myzus grey-belt pipe aphid flies according to any one of claims 4 to 7, wherein the porous water-absorbing material is one or more than two of cotton balls, non-woven fabrics and sponges.
9. The method for artificially feeding myzus grey-belt pipe aphid flies according to any one of claims 4 to 8, wherein the thickness of the sand is 0.8 to 1.5cm; the particle size of the sand is less than 1.5mm; the water content of the sand is 5-10wt%.
10. The method for artificially feeding myza virginiana in gray belt according to any one of claims 4 to 9, wherein the temperature in the feeding process is 16-32 ℃.
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| CN104222008A (en) * | 2013-06-21 | 2014-12-24 | 王伯明 | Method for artificial mass production of hoverflies |
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