CN1080991C

CN1080991C - Tech. of artificial breeding and cultivating Baihua crucian carp

Info

Publication number: CN1080991C
Application number: CN96119684A
Authority: CN
Inventors: 扬兴棋; 陈敏容; 俞小牧; 刘汉勤; 陈宏溪; 王铁辉
Original assignee: Institute of Hydrobiology of CAS
Current assignee: Institute of Hydrobiology of CAS
Priority date: 1996-11-22
Filing date: 1996-11-22
Publication date: 2002-03-20
Anticipated expiration: 2016-11-22
Also published as: CN1159281A

Abstract

The present invention relates to an artificial breeding and propagating technique of Baihua crucian which is a new variety for fresh water breeding. Pengze crucian whose chromosome number is 166 is selected as a female parent and white crucian is selected as a male parent to breed and produce Baihua crucian through the processes of artificial philtring, fecundation and hatching. The Baihua crucian bred by the method has the fertility rate of 85 to 95%, the hatching rate of 90 to 95% and the survival rate of 90 to 95%, and the growth speed is 15 to 20% faster than that of pengze crucian and 250% faster than that of common crucian; the Baihua crucian has the advantages of fine and tender meat, small abdomen, 70% of meat-containing quantity, convenient breeding and easy control. The present invention has obvious efficiency in production and breeding.

Description

A kind of crucian production of hybrid seeds and mating system thereof

The present invention relates to the genetic breeding of aquatic animal and the artificial propagation of parthenogentic development fish, the particularly production of hybrid seeds of a kind of crucian and mating system thereof.

Crucian is improved seeds of freshwater aquaculture industry, because delicious flavour, fine and tender taste thereby be subjected to liking of the masses deeply, Islamic crucian, crucian pot, iron plate crucian all are delicacies economical and practical on the dining table.

But the crucian individuality is little, and growth rate is slow, yields poorly.In order to change this situation, domestic priority is developed people such as the new object of multiple high-quality crucian cultivation, particularly Jiang of Inst. of Hydrobiology, Chinese Academy of Sciences one Gui and is worked out a kind of fast growth, individual big hybridized prussian carp.Also have the Pengzhe fish in Jiangxi in addition, be aquatic products circle and generally acknowledge.

Yet also there is a fly in the ointment on its outward appearance build, growth rate and the artificial seed.

Documents referring to:

1, the biological effect of allos sperm in the filial generation of pond crucian carp gynogenesis

Author: Jiang Yigui, Liang Shaochang, Chen Bende, Yu Hao are auspicious, Dan Shixin, Yang Delong, Lin Suien, Shen Genquan

Source: Hydrobiology collected papers, 1983.8 (1): 1-13

Imp d: nineteen eighty-three

2, a kind of good breed new varieties Pengzhe fish seed selection comprehensive report

Author: Xiong Xiaojun, Fu Yongjin, Xu Jinxing, Chen Weimin, Wang Xuejie, Liu Jinlu, Shi Daoquan

Source: Jiangxi aquatic science and technology 1990 (1): 8-13

Imp d: nineteen ninety

The producing method for seed that the purpose of this invention is to provide a kind of crucian, this method production of hybrid seeds are easily, fast growth, adaptability is strong, the bodily form good, contain the high crucian cultivation object of meat amount.

The Primary Study of Jiangxi Pengzhe fish mode of reproduction, Yang Xingqi, Chen Minrong, Yu Xiaomu, Chen Hongxi, aquatile journal, 1992,16 (3): 277-280; II, the karyotypic research of the natural gynogenesis Pengzhe fish of amphoteric. Chen Minrong, Yang Xingqi, Yu Xiaomu, Chen Hongxi.Aquatile journal .1996.20 (1): 25-31).

To achieve these goals, the present invention adopts following technical scheme:

The production of hybrid seeds of a kind of crucian and mating system, it is 166 that this producing method for seed is chosen chromosome, more than body weight 250 grams, has the natural gynogenetic Pengzhe fish of amphoteric as female parent, be sheerly red carp as the male parent with above white crucian carp of pure lines or the different genus of body weight 200 grams, adopting female parent's per kilogram injection human chorionic gonadtropin (HCG) 800～1200 units or interstitialcellstimulating hormone (ICSH) to discharge analog (LRH-A) 20～30 micrograms promotes the sexual maturity, the dosage of male parent's injection is half of female parent, with the artificial insemination method production of hybrid seeds of dry method insemination or wet method insemination.Fertilized egg hatching adopts the flowing water hatching or when its natural spawning fertilization, also can adopt the hydrostatic hatching in the pond.

Beneficial effect of the present invention:

1, three rates are higher: fertilization rate 85-95%, incubation rate 90-95%, survival rate 90-95%;

2, fast growth: under with pool cultivating condition, all sorts of flowers crucian carp grows fast 250% than Pengzhe fish fast growth 15-20% than common crucian carp;

3, the bodily form good, contain meat amount height: the bodily form is elongated, is long spindle-like, and fine and tender taste, belly are less, contain the meat amount and reach 70%;

4, producing method for seed is simple and easy to do, cultures the workman and is easy to grasp.

Accompanying drawing: all sorts of flowers crucian carp production of hybrid seeds block diagram.

Implement method of the present invention and step:

Embodiment 1, produce all sorts of flowers crucian carp with female Pengzhe fish, male white crucian carp

(1) selection of parent population and cultivation

Will select long spindle to female Pengzhe fish, the back is dark-grey black, and the physique stalwartness is no sick and wounded, the above ripe individuality of every tail 250 grams; White crucian carp milter will be selected the above individuality of 200 grams, and the physique stalwartness is no sick and wounded, the pure line cultivar that scale is complete.

The parent population of choosing is female, separately male, specially the pond reinforced cultivating is sure not to raise together with other demersal fishes, more will avoid milter to sneak in the female fishpond, is strictly on guard against at female parent population irriate and laying eggs in advance mating season.

(2) artificial seed

A, identify female parent:

By production of hybrid seeds block diagram, to detect its chromosome number earlier to female Pengzhe fish, adopt whole blood to cultivate the airing film-making, Giemsa dyeing, om observation, photomicrography, amplify back counting chromosome, the chromosome number that settles the standard is 166 Pengzhe fish, as stock female parent.

B, male parent: from the white crucian carp of cultivating, select the healthy and strong individuality that can squeeze out white seminal fluid again by production of hybrid seeds block diagram, as stock male parent.

C, artificial propagation: work as mating season, when water temperature reaches 17-21 ℃, per kilogram raun HCG injection chorionic hormone) 800-1200 unit, or LRH-A (luteinizing hormone-releasing hormone analog) 20-30 microgram.The milter injected dose reduces by half.

D, artificial insemination: by production of hybrid seeds block diagram, allos milter sperm combines with the ovum of raun, adopts dry method or wet method to inseminate, and also can allow its natural spawning, fertilization.

E, artificial incubation: fertilized egg is hatched with flowing water, and when its natural spawning fertilization, the hydrostatic hatching hatches fry in the pond.

F, seed rearing: identical with the conventional offspring seed cultivation method of carp, crucian.

Embodiment 2, produce all sorts of flowers crucian carp with female Pengzhe fish, the male red carp of different genus, and its method and step are with embodiment 1.

The effect of all sorts of flowers crucian carp that this method is produced is low than three rates of embodiment 1, is respectively: fertilization rate 65-85%, incubation rate 80-90%, survival rate 80-85%.

All sorts of flowers crucian carp seed that above embodiment produces, on probation through ground raisers such as Hubei, Guangdong, its feedback effects is remarkable.

The present invention relates to artificial seed and the raising technology thereof of the new object all sorts of flowers of freshwater aquiculture crucian carp.Choose chromosome number and be 166 Pengzhe fish and do female parent, white crucian carp does the male parent, by artificial propagation, fertilization, hatching, cultivates and produces all sorts of flowers crucian carp.With all sorts of flowers crucian carp of this kind method production of hybrid seeds, its fertilization rate is 85-95%, and incubation rate is 90-95%, survival rate 90-95%, and growth rate is than the fast 15-20% of Pengzhe fish, and is faster 250% than common crucian carp, fine and tender taste, belly is little, contains meat amount 70%, and the production of hybrid seeds is easy, easily grasps.In producing breed, remarkable benefit is arranged.

Claims

1, the production of hybrid seeds of a kind of crucian and mating system, it is characterized in that, it is 166 that this producing method for seed is chosen chromosome, more than body weight 250 grams, has the natural gynogenetic Pengzhe fish of amphoteric as female parent, be sheerly red carp as the male parent with above white crucian carp of pure lines or the different genus of body weight 200 grams, adopting female parent's per kilogram injection human chorionic gonadtropin (HCG) 800～1200 units or interstitialcellstimulating hormone (ICSH) to discharge analog (LRH-A) 20～30 micrograms promotes the sexual maturity, the dosage of male parent's injection is half of female parent, with the artificial insemination method production of hybrid seeds of dry method insemination or wet method insemination.

2, the production of hybrid seeds of a kind of crucian according to claim 1 and mating system is characterized in that, fertilized egg hatching adopts the flowing water hatching or when its natural spawning fertilization, also can adopt the hydrostatic hatching in the pond.