CN101642067B - Method for artificially hastening parturition of American reeves shads, inseminating and hatching germ cells - Google Patents
Method for artificially hastening parturition of American reeves shads, inseminating and hatching germ cells Download PDFInfo
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- CN101642067B CN101642067B CN2009100340854A CN200910034085A CN101642067B CN 101642067 B CN101642067 B CN 101642067B CN 2009100340854 A CN2009100340854 A CN 2009100340854A CN 200910034085 A CN200910034085 A CN 200910034085A CN 101642067 B CN101642067 B CN 101642067B
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- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention relates to a method for artificially hastening parturition of American reeves shads, inseminating and hatching germ cells, belonging to the technical field of fish reproduction. The method comprises the following hatching steps: preparation process of transporting ripe fishes, feeding momentarily, and the like and an artificial parturition hastening process of injecting hormone twice; completing an artificial insemination process by two persons in a matching mode; adopting a hatching process of a running water hatching device without dead angles at hatching water temperature below 16-22 DEG C; and opening in time for feeding after fries are hatched for 2-3 days. The method is reasonable and scientific, optimizes an artificial insemination technology, manufactures the running water hatching device without dead angles, has convenient and reliable operation, and can ensure the stable production of fries and better solve the problem of scale production of American reeves shad fries; and the hatching rate of the germ cells reaches above 80 percent.
Description
Technical field
The present invention relates to a kind of America hilsa herring artificial induced spawning, insemination and method for incubating oosperm, belong to the fish reproduction technical field.
Background technology
The America hilsa herring, formal name used at school Alosa sapidissima, English name American shad has another name called U.S. hilsa herring, is subordinate to Pacific herring shape catalogue clupeidae Alosa.It is the same with endangered Chinese hilsa herring (Tenualosa reevesii), and is bright, fertile, beautiful, all is anadromy migration fishes that very high economic worth is arranged.
Because the America hilsa herring is in the high market prospects of China, the domestic climax of introducing a fine variety America hilsa herring incubating oosperm, breed that started of beginning in 2003, at present, in Guangdong, tens provinces and cities such as Zhejiang, Jiangsu, Shanghai, Hubei, Hunan carry out U.S.'s hilsa herring large-scale cultivation; But the difficulty of fertilized egg continues to increase, the cultural technique that lowly adds of hatchability of fertile eggs is not very ripe owing to introduce a fine variety, and the breed scale is subjected to certain restriction.Therefore, break through the artificial propagation techniques under the pond culture condition, solve the technical bottleneck that seed supply problem of unstable becomes America hilsa herring large-scale cultivation and industrialization.
The America hilsa herring is a kind of anadromy fish, and in annual spring, through the stimulation of extrinsicfactors such as water temperature, current, sexual gland is reached maturity gradually in the migration, lays eggs in the birthplace; And in the Millerton lake of upstream, San Joaquin river, found envelope type America, land hilsa herring at present.This research group adopts measures such as fortification, hormone are thrown something and fed, stimulation by running water successfully to carry out the pond artificial propagation of America hilsa herring; Though the environmental propagation method of employings such as Liu Qinghua intensification has been carried out the breeding of America hilsa herring, does not relate to the concrete processing step of concrete artificial induced spawning, insemination and incubating oosperm.
Summary of the invention
The object of the present invention is to provide that a kind of fry that can overcome existing America hilsa herring artificial induced spawning, fertilization and method for incubating oosperm yields poorly, ropy defective; By the specially treated artificial induced spawning, avoid the scale of America hilsa herring to come off, optimize the artificial insemination step, improve fertilization rate, labour intensity can not only be reduced, and America hilsa herring artificial induced spawning, insemination and the method for incubating oosperm of incubation rate can be significantly improved.
According to technical scheme provided by the invention, a kind of America of the present invention hilsa herring artificial induced spawning, insemination and method for incubating oosperm, adopt following processing step:
1, artificial induced spawning process
(1), set-up procedure fishes for, selects maturity America hilsa herring preferably in culturing the pond, be transported to area 20m through 5 ‰ salinity phreatic waters
2, support temporarily in the depth of water 0.8-1m cement pit; And prepare hasten parturition pin and a thin towel.
(2), artificial induced spawning adopts luteotropin releasing hormone d-ala analog (LRH-A
2) and fish spawning-induced addition agent compatibility parent population is hastened parturition; Dorsal fin base portion double injection: for the first time, female America hilsa herring injected dose is: 60 μ g LRH-A
2+ 10mg fish spawning-induced addition agent/Kg, milter is not injected, and injection for the second time behind the 20-24h: female America hilsa herring injected dose is: 160 μ g LRH-A
2+ 30mg fish spawning-induced addition agent/Kg, milter is half of raun; Injection operation is finished jointly by 2 people, and the ready thin towel of a human is taken advantage of a situation and wrapped parent population, moves with water, and another people then carries out injection of hormone across the towel pin towards the head 45, and the band hydromining is operated with 2ml specification disposable sterilized injector.
2, artificial insemination process
It is 10h-15h that the oxytocic hormone injection finishes the aftereffect time, carries out the dry method of artificial fertilization; The apparatus that holds essence, ovum should be dry, clean; Get the ovum operation and finished by 2 people cooperation, the left side person gently squeezes ovary till the ovum grain can not flow out naturally from top to bottom with thumb and forefinger on ovary top; The right side person clamp-ons seminal fluid immediately and stirs evenly, sperm volume is advisable to be full of the ovum gap, drips the Chi Shui that is about the long-pending 1/5-1/3 of smart oophyte subsequently earlier and activates sperm, increases it in conjunction with the fertilization probability, with clean phreatic water washing 2～4 times, remove unnecessary seminal fluid and dirt then.
3, incubating oosperm process
(1), the hatching pail of hatching apparatus hatching apparatus employing is made of plastic;
(2), the incubation temperature incubation temperature is controlled at 16-22 ℃;
(3), hatching water quality management hatching water is water purification, guarantees between the incubation period that water quality is pure and fresh, current are unobstructed, flow velocity is controlled at 0.3-0.5m/s, in time removes egg membrane; Will reduce water flow velocity in the hatching pail behind the fry membrane, the controlled 0.1-0.2m/s that is made as consumes to prevent that fry muscle power is undue.
(4) bait is under 16-22 ℃, fry membrane 2 days, mouthpart, intestinal tube form, the monoplast green alga that filters with 150 mesh sieve thin,tough silk of should in time throwing something and feeding, wheel animalcule (in the back water body of throwing something and feeding monoplast green alga, wheel animalcule amount reach 5～8/ml) or down the summer flower cultivation is carried out on the pool.
Described hatching pail is made of plastic, is advisable about water capacity 500-700kg, puts ovum density 2.0 * 10
4-2.5 * 10
4Grain/bucket.Described hatching pail by hatching staving 1, overflow pipe 2, go out jacket pipe 3, oxygen increasing tube 4, water inlet pipe 5, micropore oxygen increasing tube 6, flush port 7 and valve control outlet pipe 8 etc. and form.In hatching staving 1, be respectively arranged with overflow pipe 2, oxygen increasing tube 4, water inlet pipe 5; Set out jacket pipe 3 in the described overflow pipe 2, overflow pipe 2 is not held to pass and is controlled outlet pipe 8 with valve at the bottom of 1 barrel of the staving of hatching and be connected; Described overflow pipe 2 is provided with aperture 2-1, and overcoat bolting silk 2-2.The external water supply plant of described water inlet pipe 5 one ends, the other end is a flush port 7; The external aerator of described oxygen increasing tube 4 one ends, another termination micropore oxygen increasing tube 6, and be close at the bottom of 1 barrel of the staving of hatching.
Described water purification is phreatic water and running water behind aeration or the Yangtze River Water of filtering through the plankton screen pack, and phreatic water is 1: 1 with running water behind aeration or the Yangtze River Water volume ratio of filtering through the plankton screen pack.Described filter screen is 80 orders.
Compared with the prior art the present invention has the following advantages:
1, reasonable, scientific methods in artificial induced spawning, insemination and hatching process, have been adopted, wherein, reduced damage every the towel injection to the America hilsa herring, improved standardization artificial insemination efficient in operation ground has improved fertilization rate, the temperature control design of hatching apparatus, flowing water, the design of no dead angle, reliably convenient, increased substantially the fertilized egg hatching rate.
2, the method that combines according to ecological principle and America hilsa herring early growth and development characteristic, the mode of the 2nd day bait of promptly throwing something and feeding behind membrane, the mistaken ideas that just begin bait throwing in after having avoided yolk sac to disappear have guaranteed the survival rate of fry; Also improved reference for the exploitation of Clupeiformes fish.
3, a whole set of technology is carried out under indoor full manual control condition; be not subjected to the external environment factor affecting, convenient and reliable operation can be guaranteed the steady production of seed; solve the large-scale production difficult problem of American shad fry and fingerlings preferably, the hatchability of fertile eggs of the technology of the present invention reaches more than 80%.
Description of drawings
Fig. 1 is a hatching apparatus overall structure schematic diagram of the present invention.
Fig. 2 is the micropore oxygenation schematic diagram of hatching apparatus of the present invention.
Fig. 3 is the overflow pipe structural representation of hatching apparatus of the present invention.
Embodiment
Embodiment during following the present invention incites somebody to action in conjunction with the accompanying drawings is further described:
Embodiment 1: a kind of America of the present invention hilsa herring artificial induced spawning, insemination and method for incubating oosperm, adopt following hatching step:
Place: base, rare fish protection research center, the Changjiang river
1, artificial induced spawning process
1. set-up procedure is cultured in the pond at 8 mu and is fished for, selects maturity America hilsa herring preferably, is transported to area 20m through 5 ‰ salinity phreatic waters
2, support temporarily in the depth of water 0.8m cement pit; And prepare the thin towel of hasten parturition a pin and a 0.4 * 0.2m.
2. artificial induced spawning adopts luteotropin releasing hormone d-ala analog (LRH-A
2) and fish spawning-induced addition agent (Ningbo Hormone product Co., Ltd.) compatibility parent population is hastened parturition.Dorsal fin base portion double injection: for the first time, female America hilsa herring injected dose is: 60 μ g LRH-A
2+ 10mg fish spawning-induced addition agent/Kg, milter is not injected, and injection for the second time behind the 24h: female America hilsa herring injected dose is: 160 μ g LRH-A
2+ 30mg fish spawning-induced addition agent/Kg, milter is half of raun.Injection operation is finished jointly by 2 people, the ready thin towel of one human is taken advantage of a situation and is wrapped parent population, move with water, another people then carries out injection of hormone across the towel pin towards the head 45, and the band hydromining is operated with 2ml specification disposable sterilized injector (production of Changzhou Yue Kang medical equipment Co., Ltd).
2, artificial insemination process
Injecting for the 2nd pin oxytocic hormone aftereffect time is 15h, carries out the dry method of artificial fertilization.The apparatus that holds essence, ovum should be dry, clean.Get the ovum operation and finished by 2 people cooperation, the left side person gently squeezes ovary till the ovum grain can not flow out naturally from top to bottom with thumb and forefinger on ovary top; The right side person clamp-ons seminal fluid immediately and stirs evenly, and sperm volume is advisable to be full of the ovum gap, drips earlier subsequently to be about 1/5 long-pending Chi Shui of smart oophyte and to activate sperm, increases it in conjunction with the fertilization probability, washes 2 times with clean phreatic water then, removes unnecessary seminal fluid and dirt.
3, incubating oosperm process
1. the described hatching pail of hatching apparatus is made of plastic, is advisable about water capacity 500kg, puts ovum density 2 * 10
4Grain/bucket.Described hatching pail by hatching staving 1, overflow pipe 2, go out jacket pipe 3, oxygen increasing tube 4, water inlet pipe 5, micropore oxygen increasing tube 6, flush port 7 and valve control outlet pipe 8 etc. and form.In hatching staving 1, be respectively arranged with overflow pipe 2, oxygen increasing tube 4, water inlet pipe 5; Set out jacket pipe 3 in the described overflow pipe 2, overflow pipe 2 is not held to pass and is controlled outlet pipe 8 with valve at the bottom of 1 barrel of the staving of hatching and be connected; Described overflow pipe 2 is provided with aperture 2-1, and overcoat 80 mesh sieve thin,tough silk 2-2.The external water supply plant of described water inlet pipe 5 one ends, the other end is a flush port 7; The external aerator of described oxygen increasing tube 4 one ends, another termination micropore oxygen increasing tube 6, and be close at the bottom of 1 barrel of the staving of hatching.Water inlet pipe 5 is fixed in overflow pipe 2 outer walls and is close to a barrel diapire, is 45 °, is micropore oxygenation at the bottom of micropore oxygen increasing tube 6 also is close to barrel, the design of no dead angle.
2. the incubation temperature incubation temperature is controlled at 16 ℃.
3. hatching water quality management hatching water is phreatic water and running water behind aeration or the water purification of forming through the Yangtze River Water (volume ratio is 1: 1) that the plankton screen pack filters.Guarantee between the incubation period that water quality is pure and fresh, current are unobstructed, and flow velocity is controlled at 0.3m/s, in time remove egg membrane; Will reduce water flow velocity in the hatching pail behind the fry membrane, the controlled 0.1m/s that is made as consumes to prevent that fry muscle power is undue.
4. bait is under 16 ℃, fry membrane 3 days, mouthpart, intestinal tube form, the monoplast green alga that filters with 150 mesh sieve thin,tough silk of should in time throwing something and feeding, wheel animalcule (in the back water body of throwing something and feeding monoplast green alga, wheel animalcule amount reach 5/ml) or down the summer flower cultivation is carried out on the pool.
Embodiment 2: a kind of America of the present invention hilsa herring artificial induced spawning, insemination and method for incubating oosperm, adopt following hatching step:
Place: Jiangyin Shengang Sanxian Cultivation Co., Ltd.
1, artificial induced spawning process
1. set-up procedure is cultured in the pond at 8 mu and is fished for, selects maturity America hilsa herring preferably, is transported to area 20m through 5 ‰ salinity phreatic waters
2, support temporarily in the depth of water 0.9m cement pit; And prepare the thin towel of hasten parturition a pin and a 0.4 * 0.2m.
2. artificial induced spawning adopts luteotropin releasing hormone d-ala analog (LRH-A
2) and fish spawning-induced addition agent (Ningbo Hormone product Co., Ltd.) compatibility parent population is hastened parturition.Dorsal fin base portion double injection: for the first time, female America hilsa herring injected dose is: 60 μ g LRH-A
2+ 10mg fish spawning-induced addition agent/Kg, milter is not injected, and injection for the second time behind the 22h: female America hilsa herring injected dose is: 160 μ g LRH-A
2+ 30mg fish spawning-induced addition agent/Kg, milter is half of raun.Injection operation is finished jointly by 2 people, the ready thin towel of one human is taken advantage of a situation and is wrapped parent population, move with water, another people then carries out injection of hormone across the towel pin for 45 ° towards head, and the band hydromining is operated with 2ml specification disposable sterilized injector (production of Changzhou Yue Kang medical equipment Co., Ltd).
2, artificial insemination process
Injecting for the 2nd pin oxytocic hormone aftereffect time is 13h, carries out the dry method of artificial fertilization.The apparatus that holds essence, ovum should be dry, clean.Get the ovum operation and finished by 2 people cooperation, the left side person gently squeezes ovary till the ovum grain can not flow out naturally from top to bottom with thumb and forefinger on ovary top; The right side person clamp-ons seminal fluid immediately and stirs evenly, and sperm volume is advisable to be full of the ovum gap, drips earlier subsequently to be about 1/4 long-pending Chi Shui of smart oophyte and to activate sperm, increases it in conjunction with the fertilization probability, washes 3 times with clean phreatic water then, removes unnecessary seminal fluid and dirt.
3, incubating oosperm process
1. the hatching pail of hatching apparatus hatching apparatus employing is made of plastic, whole barrel-shaped for boring end cylinder, be plastic material, central authorities in the bucket conical bottom are provided with delivery port, delivery port is connected with the discharge nozzle of band valve downwards, also be provided with the vertical boring overflow pipe that connects bucket end groove in the bucket end central authorities, inboard cover sleeve pipe not with holes, outer wall then wraps tightly 80 mesh sieve thin,tough silk; External water inlet pipe is fixed in the overflow pipe outer wall and is close to a barrel diapire, is 45 °, is micropore oxygenation at the bottom of external oxygen increasing tube also is close to barrel, the design of no dead angle.
1. the described hatching pail of hatching apparatus is made of plastic, is advisable about water capacity 600kg, puts ovum density 2.3 * 10
4Grain/bucket.Structure is identical with embodiment one.
2. the incubation temperature incubation temperature is controlled at 19 ℃.
3. hatching water quality management hatching water is phreatic water and running water behind aeration or the water purification of forming through the Yangtze River Water (volume ratio is 1: 1) that the plankton screen pack filters.Guarantee between the incubation period that water quality is pure and fresh, current are unobstructed, and water flow velocity is controlled at 0.4m/s, in time remove egg membrane; Will reduce water flow velocity in the hatching pail behind the fry membrane, the controlled 0.15m/s that is made as consumes to prevent that fry muscle power is undue.
4. bait is under 19 ℃, fry membrane 2 days, mouthpart, intestinal tube form, the monoplast green alga that filters with 150 mesh sieve thin,tough silk of should in time throwing something and feeding, wheel animalcule (in the back water body of throwing something and feeding monoplast green alga, wheel animalcule amount reach 6/ml) or down the summer flower cultivation is carried out on the pool.
Hasten parturition 3 batch by above-mentioned implementation step and requirement on April 25th, 2008 to May 5,15 groups of kind of the fishes of hastening parturition, The mean spawning rate 75%; Hatchability of fertile eggs 88%.
Embodiment 3: a kind of America of the present invention hilsa herring artificial induced spawning, insemination and method for incubating oosperm, adopt following hatching step:
Place: base, aquatic products director, Jiangyin City
1, artificial induced spawning process
1. set-up procedure is cultured in the pond at 8 mu and is fished for, selects maturity America hilsa herring preferably, is transported to area 20m through 5 ‰ salinity phreatic waters
2, support temporarily in the depth of water 1.0m cement pit; And prepare the thin towel of hasten parturition a pin and a 0.4 * 0.2m.
2. artificial induced spawning adopts luteotropin releasing hormone d-ala analog (LRH-A
2) and fish spawning-induced addition agent (Ningbo Hormone product Co., Ltd.) compatibility parent population is hastened parturition.Dorsal fin base portion double injection: for the first time, female America hilsa herring injected dose is: 60 μ g LRH-A
2+ 10mg fish spawning-induced addition agent/Kg, milter is not injected, and injection for the second time behind the 20h: female America hilsa herring injected dose is: 160 μ g LRH-A
2+ 30mg fish spawning-induced addition agent/Kg, milter is half of raun.Injection operation is finished jointly by 2 people, the ready thin towel of one human is taken advantage of a situation and is wrapped parent population, move with water, another people then carries out injection of hormone across the towel pin towards the head 45, and the band hydromining is operated with 2ml specification disposable sterilized injector (production of Changzhou Yue Kang medical equipment Co., Ltd).
2, artificial insemination process
Injecting for the 2nd pin oxytocic hormone aftereffect time is 10h, carries out the dry method of artificial fertilization.The apparatus that holds essence, ovum should be dry, clean.Get the ovum operation and finished by 2 people cooperation, the left side person gently squeezes ovary till the ovum grain can not flow out naturally from top to bottom with thumb and forefinger on ovary top; The right side person clamp-ons seminal fluid immediately and stirs evenly, and sperm volume is advisable to be full of the ovum gap, drips earlier subsequently to be about 1/3 long-pending Chi Shui of smart oophyte and to activate sperm, increases it in conjunction with the fertilization probability, washes 4 times with clean phreatic water then, removes unnecessary seminal fluid and dirt.
3, incubating oosperm process
1. the described hatching pail of hatching apparatus is made of plastic, is advisable about water capacity 700kg, puts ovum density 2.5 * 10
4Grain/bucket.Structure is identical with embodiment one.
2. the incubation temperature incubation temperature is controlled at 22 ℃.
3. hatching water quality management hatching water is phreatic water and running water behind aeration or the water purification of forming through the Yangtze River Water (volume ratio is 1: 1) that the plankton screen pack filters.Guarantee between the incubation period that water quality is pure and fresh, current are unobstructed, and water flow velocity is controlled at 0.5m/s, in time remove egg membrane; Will reduce water flow velocity in the hatching pail behind the fry membrane, the controlled 0.2m/s that is made as consumes to prevent that fry muscle power is undue.
4. bait is under 22 ℃, fry membrane 2 days, mouthpart, intestinal tube form, the monoplast green alga that filters with 150 mesh sieve thin,tough silk of should in time throwing something and feeding, wheel animalcule (in the back water body of throwing something and feeding monoplast green alga, wheel animalcule amount reach 8/ml) or down the summer flower cultivation is carried out on the pool.
Hasten parturition 3 batch by above-mentioned implementation step and requirement on May 6th, 2008 to May 15,15 groups of kind of the fishes of hastening parturition, The mean spawning rate 70%; Hatchability of fertile eggs 80%.
Claims (2)
1. an America hilsa herring artificial induced spawning, insemination and method for incubating oosperm is characterized in that: adopt following hatching step:
(1), artificial induced spawning process
(a), set-up procedure: in culturing the pond, fish for, select the good America hilsa herring of maturity, be transported to area 20m through 5 ‰ salinity phreatic waters
2, support temporarily in the depth of water 0.8-1m cement pit; And prepare hasten parturition pin and a thin towel;
(b), artificial induced spawning: adopt luteotropin releasing hormone d-ala analog LRH-A
2With fish spawning-induced addition agent compatibility parent population is hastened parturition; Dorsal fin base portion double injection: for the first time, female America hilsa herring injected dose is: 60 μ g LRH-A
2+ 10mg fish spawning-induced addition agent/Kg, milter is not injected, and injection for the second time behind the 20-24h: female America hilsa herring injected dose is: 160 μ g LRH-A
2+ 30mg fish spawning-induced addition agent/Kg, milter is half of raun;
(2), artificial insemination process
Inject for the second time luteotropin releasing hormone d-ala analog LRH-A
2With the effect time of fish spawning-induced addition agent be 10h-15h, carry out the dry method of artificial fertilization; Ovum grain and seminal fluid stir evenly, and sperm volume is as the criterion to be full of the ovum gap, and the Chi Shui that drips earlier to the long-pending 1/5-1/3 of smart oophyte activates sperm subsequently, with clean phreatic water washing 2-4 time, remove unnecessary seminal fluid and dirt then;
(3), incubating oosperm process
(a), hatching apparatus: it is the 500-700kg hatching pail that hatching apparatus adopts water capacity; Put ovum density 2.0 * 10
4-2.5 * 10
4Grain/bucket;
(b), incubation temperature: incubation temperature is controlled at 16-22 ℃;
(c), hatching water quality management: the hatching water is a water purification, and water flow velocity is controlled at 0.3-0.5m/s before the fry membrane, in time removes egg membrane; Will reduce water flow velocity in the hatching pail behind the fry membrane, water flow velocity is controlled to be 0.1-0.2m/s, consumes to prevent that fry muscle power is undue;
Described water purification is phreatic water and running water behind aeration or the Yangtze River Water of filtering through the plankton screen pack, and phreatic water is 1: 1 with running water behind aeration or the Yangtze River Water volume ratio of filtering through the plankton screen pack, and described plankton screen pack is 80 orders;
(d) bait: under 16-22 ℃ of water temperature, fry membrane 2～3 days, mouthpart, intestinal tube form, monoplast green alga or the wheel animalcule that should in time throw something and feed and filter with 150 mesh sieve thin,tough silk, monoplast green alga or wheel animalcule amount reach 5-8/ml in the back water body of throwing something and feeding.
2. a kind of America according to claim 1 hilsa herring artificial induced spawning, insemination and method for incubating oosperm, it is characterized in that: described injection operation is taken advantage of a situation with thin towel and is wrapped parent population, move with water, and carrying out injection of hormone for 45 ° towards head across the towel pin, the band hydromining is with the operation of 2ml specification disposable sterilized injector.
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| CN2009100340854A CN101642067B (en) | 2009-08-21 | 2009-08-21 | Method for artificially hastening parturition of American reeves shads, inseminating and hatching germ cells |
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| CN2009100340854A CN101642067B (en) | 2009-08-21 | 2009-08-21 | Method for artificially hastening parturition of American reeves shads, inseminating and hatching germ cells |
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| CN103314907A (en) * | 2013-06-21 | 2013-09-25 | 太仓年年有渔农业科技有限公司 | Hatching device for chrtax quadricarinatus |
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| CN101884310B (en) * | 2010-07-01 | 2012-02-29 | 中国水产科学研究院珠江水产研究所 | Method for prompting pond-bred alosa sapidissima to spawn by ecological regulation and control |
| CN101946860A (en) * | 2010-08-09 | 2011-01-19 | 中国水产科学研究院长江水产研究所 | Bait fed in feeding habit conversion period of reeves shad fish larva |
| CN102428880B (en) * | 2010-09-29 | 2014-03-05 | 中国水产科学研究院淡水渔业研究中心 | Paracanthobrama guichenoti bleeker germ cell incubation method, as well as fry hatching method |
| CN107295987B (en) * | 2017-07-11 | 2020-10-23 | 深圳市龙岐庄实业发展有限公司 | Method for artificially induced spawning of egg-shaped pompano |
| CN107646763A (en) * | 2017-11-10 | 2018-02-02 | 中国水产科学研究院南海水产研究所 | A kind of macula lutea basket fish artificial fecundation method |
| CN110419471B (en) * | 2019-09-05 | 2021-11-16 | 中国水产科学研究院淡水渔业研究中心 | Method for artificial insemination of wild burmese reeves shad and hatching of fertilized eggs |
| CN110432192B (en) * | 2019-09-10 | 2021-07-16 | 上海市水产研究所（上海市水产技术推广站） | Method for prolonging artificial propagation time of alosa sapidissima |
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| CN1875694A (en) * | 2006-07-05 | 2006-12-13 | 刘青华 | An ecological method for breeding American reeves shad |
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