CN101836613A - Acipenser sinensis living body nontraumatic egg taking method - Google Patents
Acipenser sinensis living body nontraumatic egg taking method Download PDFInfo
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- CN101836613A CN101836613A CN201010184627A CN201010184627A CN101836613A CN 101836613 A CN101836613 A CN 101836613A CN 201010184627 A CN201010184627 A CN 201010184627A CN 201010184627 A CN201010184627 A CN 201010184627A CN 101836613 A CN101836613 A CN 101836613A
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- ovum
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses an Acipenser sinensis living body nontraumatic egg taking method, which comprises the following steps: 1, during the induced spawning of Acipenser sinensis, controlling the water level in a spawning inducing pool to be 40 to 60 centimeters; 2, placing an Acipenser sinensis egg collecting bed with an inclined tabletop in the spawning inducing pool, lifting a female Acipenser sinensis to the egg collecting bed with a Acipenser sinensis stretcher, making the fish belly of the Acipenser sinensis upward and slightly inclined toward one side for convenient artificial egg collection; 3, placing the tail part of the female Acipenser sinensis at a higher position on the inclined plane of the egg collecting bed and the head part of the at a lower position on the inclined plane of the egg collecting bed to make the tail part higher than the head part which is put in water with the mouth and gill parts underwater for ensuring the gills of the parent fish can move flexible and keep smooth breath of the living body in an egg collection process; 4, making mature fish eggs enter an oviduct from the horn mouth of the oviduct through auxiliary pressing and making the eggs in the oviduct move forward toward a genital pore; and 5, pressing the belly up and down and back and forth at the part, close to anal fins, of the belly of the fish body to promote the eggs to continue to move toward the genital pore from the oviduct and then move out of the body.
Description
Technical field
The present invention relates to a kind of mandarin sturgeon live body does not have wound and adopts the ovum method.
Background technology
Sturgeon section fish are compared with common fishes such as Cyprinidae, Nian sections, have very big-difference in the genital system configuration aspects.The ovary that mainly shows as common fish has adventitia bag quilt, is commonly called as oopod, and its lower end and oviduct are communicated to gonopore.The back ovum grain of hastening parturition is free in the oopod, when being in the low position state of head height tail since action of gravity can excrete naturally.The ovary of sturgeon section fish does not then have adventitia bag quilt, and oviduct is down the horn-like middle part, abdominal cavity that is opened on, and behind artificial induced spawning, ripe fish-egg comes off naturally from ovary and is free on the abdominal cavity, and the horn mouth from the middle part, abdominal cavity enters oviduct again.If method ovum pick-up routinely is difficult to by action of gravity fish-egg be excreted.
China starts late in the research aspect the artificial propagation of sturgeons, uses for reference mainly that sturgeon kind such as the former Soviet Union is abundant, the experience of sturgeon cultivation industry more developed country.Particularity in view of sturgeons genital system structure, all adopt to cut open the belly for the sturgeons artificial propagation both at home and abroad at present and adopt the mode of ovum, wherein the technology of the U.S. is comparatively ripe, can make the small-sized sturgeon underwent operative such as paddlefish of adopting ovum through the cutting open the belly rear section survival of sewing up a wound.And other countries adopt ovum through cutting open the belly after female sturgeon all can be dead.Mandarin sturgeon is huge because of its individuality, all adopts to cut open the belly in the past and adopts ovum artificial insemination mode, and postpartum, raun can't survive, and this protection to endangered species mandarin sturgeon resource is and is disadvantageous.
Existing technology mainly contains following shortcoming:
The mode of (1) cutting open the belly is carried out ovum artificial collection, and this technology complicated operation falls behind, can be to the mortal injury that causes of parent population.
(2) mode of cutting open the belly is carried out ovum artificial collection, adopts ovum and relies on experienced technical staff opportunity fully, holds for those skilled in the art are difficult, usually misses a large amount of losses of hastening parturition the best opportunity and causing parent population.
(3) after female sturgeon is adopted ovum through cutting open the belly, except that the small-sized sturgeon of minority can survive by operation stitching, the overwhelming majority female sturgeon death in postpartum, this is totally unfavorable to carrying out of sturgeon artificial propagation, research and protection work.
Summary of the invention
In order to overcome above-mentioned defective, the invention provides a kind of mandarin sturgeon live body does not have wound and adopts the ovum technology, when guaranteeing the smooth egg reproduction of the female parent population of mandarin sturgeon, reduces injury of female close sturgeon and the technical method that makes it survive and.
The present invention adopts following technical scheme to realize:
A kind of mandarin sturgeon live body does not have wound and adopts the ovum method, and it comprises the steps:
When (1) the mandarin sturgeon raun lays eggs, the water level of spawning inducing pool is controlled at 40～60cm;
(2) mandarin sturgeon is adopted the ovum bed and placed in the spawning inducing pool, with the sturgeon stretcher mandarin sturgeon raun is transferred to and adopted on the ovum bed, make the mandarin sturgeon raun outside of belly up, lateral deviation slightly is so that ovum artificial collection;
(3) afterbody of mandarin sturgeon raun is positioned at and adopts the higher end in ovum bed inclined-plane, head is positioned at a lower end of adopting ovum bed surface inclined-plane, form the high low posture of tail, head immerses in the entry, the mouth and the gill are positioned at below the water surface, and the gill that guarantees parent population can activity freely, to guarantee that it can be breathed freely in adopting the ovum process;
(4) make ripe fish-egg enter oviduct by auxiliary extruding, and make the fish-egg in the oviduct continue to move to gonopore from oviducal horn mouth;
(5) be close to the nearly anal fin of fish body outside of belly place, push the outside of belly up and down back and forth, impel the ovum grain to continue to move and excrete to the gonopore direction from oviduct.
The present invention has following advantage:
(1) the present invention is guaranteeing in the smooth egg reproduction of the female sturgeon of ripe mandarin sturgeon of artificial induced spawning; can also guarantee the survival of female sturgeon in postpartum; thereby the present situation of " having killed fish and having got ovum " before having changed fully has great importance to protecting endangered mandarin sturgeon species resource more effectively.
(2) the present invention is equally applicable to other kind in the existing 27 kinds of sturgeon section fish in the world, and this invention will be for will make these rare species artificial breeding protection aspects have breakthrough progress.
This adopts the artificial induced spawning breeding of ovum method 2000～2008 years wild mandarin sturgeon parent populations, and mandarin sturgeon total man worker mandarin sturgeon parent population in 2009 is hastened parturition in the breeding, obtained good application, the ripe female sturgeon of mandarin sturgeon is through artificial induced spawning and after implementing ovum pick-up, its postpartum, the survival rate of female sturgeon reached 100%, and the artificial propagation of mandarin sturgeon has all obtained success.
Specific embodiment
Describe technical scheme of the present invention in detail below in conjunction with embodiment.
1, principle:
The ripe raun of mandarin sturgeon is behind artificial induced spawning, ripe fish-egg has directly entered body cavity, by the high low posture of artificial control mandarin sturgeon tail, make the ripe fish-egg that is free on body cavity converge at the near first side of body cavity, and the internal organs of near side of body cavity are more, the space that holds free ovum grain is limited, therefore, free ripe fish-egg mainly concentrates on around the oviducal horn mouth in middle part, abdominal cavity, by reasonably manually assisting the extruding means, can impel ripe fish-egg to enter oviduct, and successfully excrete from gonopore by oviducal horn mouth.
2, mandarin sturgeon is adopted the design of ovum bed
Mandarin sturgeon is adopted the ovum bed and is similar to rectangular table, its material can be for wooden, also can be iron and steel, four table legs are two long two weak points, and wherein the long leg height is 60～80cm, high 20～the 30cm of short-leg, wide 80～the 120cm of desktop, the long 300～350cm of desktop adopts the ovum bed and adopts the ovum bed surface and be 10～30 ° of gradients thereby make, spread the thick sponge of 5～10cm adopting to install additional on the ovum bed surface, and with wide strap or transparent adhesive tape be fixed on adopt on the ovum bed surface standby.
3, control of the position of mandarin sturgeon ovum pick-up and parent population keep-alive oxygenation method
After confirming that the mandarin sturgeon raun lays eggs, water level with spawning inducing pool is controlled at 40～60cm immediately, subsequently mandarin sturgeon is adopted the ovum bed and is placed spawning inducing pool, mandarin sturgeon is lifted to the special-purpose stretcher of sturgeon adopted on the ovum bed, with adopt the ovum bed surface and be placed in parallel in and adopt on the ovum bed surface, and parent population controlled, the parent population outside of belly up, lateral deviation slightly, so that ovum artificial collection, and notice that afterbody is positioned at the higher position of adopting ovum bed surface inclined-plane, head forms the high low posture of tail at the lower position of adopting ovum bed surface inclined-plane.Head stretches into downwards in the water, makes mouthful and gill portion is positioned at below the water surface, guarantee the gill of parent population can activity freely, smooth and easy to keep it in the ovum pick-up process, to breathe, but also the indirect labor to parent population mouthful in pour water, to be reached for the purpose of parent population keep-alive oxygenation.
4, the auxiliary pressing method of mandarin sturgeon ovum pick-up
Mandarin sturgeon in natural propagation the fish body by adverse current ceaselessly move about, the coordinated movement of various economic factors such as jump excretes the injection oviduct import in batches of ripe fish-egg.Therefore, artificial ovum pick-up also should adopt indirect labor's pressing method to imitate the behavior of mandarin sturgeon natural propagation as far as possible, short its smooth ovulation.
Carrying out the auxiliary extruding of mandarin sturgeon ovum pick-up needs two people to coordinate to carry out, one of them people is positioned at the middle part in mandarin sturgeon left side, main responsible ripe fish-egg enters oviduct by auxiliary extruding from oviducal horn mouth, and makes the fish-egg in the oviduct continue to move to gonopore.Its pressing method is: two hands are stretched out, the centre of the palm is downward, the right hand is positioned at the venter middle front part, left hand is positioned at the venter postmedian, two hands one in front and one in back are close to the mandarin sturgeon outside of belly and are pushed belly up and down back and forth, promote free fish-egg continuously to pour into oviduct, under the follow-up promotion that pours into fish-egg, impel to pour into oviducal fish-egg in advance and move to gonopore passively by manpower; Another person then is positioned at the postmedian on mandarin sturgeon right side, and mainly responsible fish-egg is the mobile of the nearly reproduction nose end of oviduct and impel fish-egg to excrete from gonopore.Its main method is: the right hand is stretched out, and the centre of the palm is downward, is close to the nearly anal fin of fish body outside of belly place, pushes the outside of belly up and down back and forth, impels the ovum grain to continue to move and excrete to the gonopore direction from oviduct.In extrusion process, two people should work in coordination, the pushing of two people pickpockets should be followed free fish-egg and progressively be entered oviduct from horn mouth, and carry out in tandem by the movement law that oviduct excretes, specific practice is that press earlier the outside of belly belly centre of at first first people's right hand mandarin sturgeon, the right hand press also do not get up before, the left hand and then postmedian of the outside of belly belly of mandarin sturgeon is pressed, this people's left hand press also do not get up before, another one people's the right hand is pressed near the outside of belly anal fin of mandarin sturgeon; Before this people's the right hand got up, first the right hand was pressed again, so repeated to get final product.
Adopt this to adopt the ovum method and on the paddlefish whole artificial propagation of the acipenser schrencki whole artificial propagation in 2008～2009 years of this institute and 2001～2009 years, obtained good application, the ovulation rate of acipenser schrencki and the female sturgeon of paddlefish is all more than 90%, the ovum grain of discharging is neat, maturity good, whole artificial propagation respond well, postpartum, the survival rate of female sturgeon was 100%.
Claims (2)
1. a mandarin sturgeon live body does not have wound and adopts the ovum method, and it comprises the steps:
(1) after the mandarin sturgeon raun laid eggs, the water level with spawning inducing pool was controlled at 40～60cm immediately;
(2) subsequently mandarin sturgeon is adopted the ovum bed and placed spawning inducing pool, adopt the ovum bed surface and tilt, the mandarin sturgeon raun is lifted to the sturgeon stretcher adopted on the ovum bed, make the mandarin sturgeon raun outside of belly up, lateral deviation is convenient to ovum artificial collection slightly;
(3) afterbody that makes the mandarin sturgeon raun is above the desktop inclined-plane, and head forms the high low posture of tail below the desktop inclined-plane, head stretches in the water downwards, make mouthful and gill portion is positioned at below the water surface, guarantee the gill of parent population can activity freely, smooth and easy to keep it in the ovum pick-up process, to breathe;
(4) make ripe fish-egg enter oviduct by auxiliary extruding, and make the fish-egg in the oviduct continue to move to gonopore from oviducal horn mouth;
(5) be close to the nearly anal fin of fish body outside of belly place, push the outside of belly up and down back and forth, impel the ovum grain to continue to move and excrete to the gonopore direction from oviduct.
2. the method for claim 1, it is characterized in that the material that described mandarin sturgeon is adopted the ovum bed is wooden or iron and steel, four table legs two long two are short, wherein the long leg height is 60～80cm, high 20～the 30cm of short-leg adopts the wide 80～120cm of ovum bed surface, long 300～350cm, when adopting the ovum bed and keeping flat, adopt the ovum bed surface horizontal by 10～30 ° angle, adopting the thick sponge of ovum bed surface upper berth 5～10cm, and fixing with wide strap or transparent adhesive tape.
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| CN201010184627A CN101836613A (en) | 2010-05-27 | 2010-05-27 | Acipenser sinensis living body nontraumatic egg taking method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102440202A (en) * | 2011-09-29 | 2012-05-09 | 中国水产科学研究院黑龙江水产研究所 | Screening method for roes of fishes in Acipenseridae |
| CN103125990A (en) * | 2011-11-24 | 2013-06-05 | 甘肃省渔业技术推广总站 | Trout roe production method |
| CN109892259A (en) * | 2019-03-28 | 2019-06-18 | 贵州大学 | A kind of sturgeon breeding parent population is fixed and fish-egg collection device |
| CN111493003A (en) * | 2020-04-17 | 2020-08-07 | 佛山精迅能冷链科技有限公司 | Semi-immersion type living body aquatic product storage method |
Citations (2)
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| CN101120660A (en) * | 2007-08-28 | 2008-02-13 | 中国水产科学研究院黄海水产研究所 | Method for inducing female nucleus growth with heterologous frozen sperm |
| CN101595849A (en) * | 2009-06-23 | 2009-12-09 | 中国水产科学研究院黄海水产研究所 | Flounder tetraplont fry batch induction method |
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2010
- 2010-05-27 CN CN201010184627A patent/CN101836613A/en active Pending
Patent Citations (2)
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| CN101120660A (en) * | 2007-08-28 | 2008-02-13 | 中国水产科学研究院黄海水产研究所 | Method for inducing female nucleus growth with heterologous frozen sperm |
| CN101595849A (en) * | 2009-06-23 | 2009-12-09 | 中国水产科学研究院黄海水产研究所 | Flounder tetraplont fry batch induction method |
Non-Patent Citations (1)
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102440202A (en) * | 2011-09-29 | 2012-05-09 | 中国水产科学研究院黑龙江水产研究所 | Screening method for roes of fishes in Acipenseridae |
| CN102440202B (en) * | 2011-09-29 | 2013-06-19 | 中国水产科学研究院黑龙江水产研究所 | Screening method for roes of fishes in Acipenseridae |
| CN103125990A (en) * | 2011-11-24 | 2013-06-05 | 甘肃省渔业技术推广总站 | Trout roe production method |
| CN103125990B (en) * | 2011-11-24 | 2014-09-03 | 甘肃省渔业技术推广总站 | Trout roe production method |
| CN109892259A (en) * | 2019-03-28 | 2019-06-18 | 贵州大学 | A kind of sturgeon breeding parent population is fixed and fish-egg collection device |
| CN111493003A (en) * | 2020-04-17 | 2020-08-07 | 佛山精迅能冷链科技有限公司 | Semi-immersion type living body aquatic product storage method |
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Application publication date: 20100922 |