CN202680227U - Loach germ cell incubating device - Google Patents

Loach germ cell incubating device Download PDF

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Publication number
CN202680227U
CN202680227U CN2012201179299U CN201220117929U CN202680227U CN 202680227 U CN202680227 U CN 202680227U CN 2012201179299 U CN2012201179299 U CN 2012201179299U CN 201220117929 U CN201220117929 U CN 201220117929U CN 202680227 U CN202680227 U CN 202680227U
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China
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loach
loaches
jar
pipe
inflation
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Expired - Fee Related
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CN2012201179299U
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Chinese (zh)
Inventor
储张杰
吴有先
王伟洪
龚丽贞
钟爱华
卢国兴
卢建达
白晓倩
陈永强
黄文文
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Zhejiang Ocean University ZJOU
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Zhejiang Ocean University ZJOU
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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Abstract

The utility model discloses a loach germ cell incubating device which comprises a cylinder, an aerating device and a water inlet pipe. The loach germ cell incubating device is characterized in that a fry discharging pipe is arranged at the bottom of the cylinder, the tail end of the fry discharging pipe is bent downward, a control valve is arranged on the fry discharging pipe, a oviposition mesh bag is installed inside a cylinder port of the cylinder, and an overflow pipe is arranged outside the cylinder port of the cylinder. Integration production of copulation oviposition and incubation is achieved, operation convenience is achieved, incubated loach fries are good in adaptability for the outside environment, and survival rate is high. The loach germ cell incubating device overcomes the defect that the loach fries incubated in a greenhouse are poor in adaptability for the outside environment, reduces breeding production links, further reduces breeding cost of loaches, and improves breeding benefits of the loaches.

Description

Loach oosperm hatching apparatus
Technical Field
The utility model relates to a device that is used for loach to breed, especially a embryonated egg hatching apparatus of loach.
Background
Loaches are famous and high-quality fishes widely distributed in China, are delicious in taste, tender in meat quality, rich in nutrition and high in value in medicine, and are deeply favored by consumers. Loaches have become important aquaculture varieties due to strong adaptability, few diseases, high survival rate, strong reproductive capacity, convenient transportation and easy availability of feed, the market price is turned over for several times, and the loaches tend to continue to rise, are one of important aquatic products exported by foreign trade in China, and the ultrahigh benefit of artificial loaches cultivation has attracted general attention of vast culturists and various social circles.
In recent years, as the loach culture area is enlarged and the demand is greatly increased, wild fries cannot meet the culture demand, and factory artificial breeding or soil pond breeding is developed in various places, which plays an important role in relieving the shortage of loach fries in China.
The industrial breeding is carried out in an indoor cement pond, the incubation temperature of the loaches is artificially controlled by heating with a boiler, the breeding yield is high, but the breeding seedlings cultivated by controlling the temperature have poor adaptability to the external environment, the survival rate of the commercial loaches during the breeding of the loaches is low, and the cost of the produced loach seedlings is high; the soil pond seedling raising has good adaptability to the environment and high survival rate, but needs a large amount of water surface resources, and in addition, the yield is relatively low, and batch selective hybridization breeding cannot be carried out.
In order to improve the yield, artificial breeding methods have been developed, for example, chinese patent application with publication number CN102217560A discloses an artificial breeding method for loach fry, which comprises the following steps: (1) selecting healthy parent loaches of more than 2 winter years, and carrying out intensified cultivation for 25-35 days according to the following method: opening male and female parent loaches in a special pond for intensive cultivation, wherein the feed adopted in the intensive cultivation is soybean meal or loach feed with the protein content of more than 50%, and a proper amount of yeast powder and vitamins are added, and the daily feeding amount is 5-7% of the weight of the parent loaches; (2) artificial hastening parturition: the oxytocic is prepared by mixing and injecting chorionic gonadotropin (HCG) and domperidone maleate (DOM), wherein the injection dosage of female loach (HCG) is 15-20IU/g, and the injection dosage of DOM is 0.003-0.005 mg/g; the injection dosage of the male loaches is 40-60% of that of the female loaches; the injection dosage of each parent loach is not more than 0.5 ml; the quantity ratio of the female loaches to the male loaches is 1: 2-3; (3) collecting fertilized eggs: distributing the parent loaches subjected to induced spawning into each spawning pond according to the sequence, wherein 20-30 parent loaches are distributed in each square meter, and standing still for spawning; after 10-14 hours after spawning induction, draining water in the spawning pond, and collecting fertilized eggs at a water outlet by using a net cage; (4) artificial hatching of fertilized eggs: transferring the collected fertilized eggs into an incubation barrel for air inflation incubation, wherein the incubation water temperature is 20-25 ℃, and incubating to obtain larval fish; (5) larva cultivation: after the fry is cultivated for 60-80 hours, the fry is intensively cultivated for 6-10 days according to the following method to obtain the loach fry: feeding yolk and rotifer during intensive cultivation, wherein daily feeding amount accounts for 25-35% of the body weight; continuously aerating in the process of larva cultivation, wherein the water temperature is 20-25 ℃.
According to the breeding method, the parent loach is not comprehensive in nutrition in the intensified breeding stage, the egg carrying amount of the loach is influenced, and the cost is increased; the induced spawning fertilization drug induced spawning drug only comprises HCG and domotone maleate DOM, the artificial induced spawning effect is not ideal, even about 25 percent of loaches cannot spawn, and the cost is high; the number of the male loaches is more than that of the female loaches, and the ratio of the number of the male loaches to the number of the female loaches leads to higher cost.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that a fertilized egg hatching device of loach is provided, the integration operation of realizing laying eggs and hatching, simple to use.
The utility model provides a technical scheme that above-mentioned technical problem adopted does: the utility model provides a loach embryonated egg hatching apparatus, hatching apparatus includes jar, aerating device and inlet tube, its characterized in that, the jar bottom sets up row's seedling pipe, row's seedling pipe end downwarping be provided with the control valve on the row's seedling pipe, be equipped with the mesh bag of laying eggs in the jar mouth of jar, be close to the jar mouth outside of jar is equipped with the warning pipe.
The net bag upper mouth of laying eggs is provided with the lid net, the net bag of laying eggs is made by polyvinyl chloride for parent loach can mate on the net bag of laying eggs and lay eggs.
The inflation device comprises an inflation tube extending into the bottom of the hatching jar, an inflation switch arranged on the inflation tube and an inflation stone at the tail end of the inflation tube, and the inflation amount can be adjusted through the inflation switch, so that dissolved oxygen in the hatching jar can be adjusted.
The number of the overflow pipes is 2-4, the overflow pipes comprise plastic bent pipes and silk screens, and the water quantity in the hatching tank is adjusted.
Compared with the prior art, the utility model has the advantages of: the integrated production of mating, oviposition and incubation is realized, the operation is convenient, the adaptability of the bred loach fries to the external environment is good, the survival rate is high, and the defect that the loach fries bred in a greenhouse have poor adaptability to the external environment is overcome; the breeding production links are reduced, the breeding cost of the loaches is further reduced, and the breeding benefit of the loaches is improved.
Drawings
Fig. 1 shows the fertilized egg hatching device of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the following embodiments.
A loach hybridization breeding method comprises the following steps:
selection of parent loaches
Selecting loaches of which the ages are 3-4, and the weight of each loach is 15-20g female, preferably 20g female, and 8-12g male, preferably 12g male. If the parent loaches are too young, the brood amount is small, and if the parent loaches are too young, the quality of produced sperms and eggs is poor, and the fertility rate is low; in addition, loach male and female parents are required to be from different regions, so that the inbreeding can be avoided as much as possible, the first filial generation can embody the heterosis, and the growth speed is higher. The female loach and the male loach can be selected according to the shape of the pectoral fin and the body surface gender characteristic, wherein the pectoral fin of the male loach is large, the second fin is the longest, the front end of the male loach protrudes in a pointed shape, the pectoral fin of the female loach is small, and the front end of the female loach is blunt and is unfolded in a fan shape. In the process of selecting loach parents, the following points are required to be achieved: 1. selecting with water to avoid parent loaches from being injured; 2. strong and lively without trauma; 3. the female loach has obvious ovary outline. And (3) separating the selected male and female loaches according to the male and female, respectively placing the male and female loaches into parent breeding ponds, breeding 200kg of parent loaches in each pond, and placing 4 ponds in total.
Wherein,the parent breeding pond is required to be a pond with no pollution, convenient traffic and sufficient electric power. Pool area 300m2-600m2The water depth is 0.8m-1.2 m. The bottom of the pool is made of mud, the thickness of the mud is 15cm-20cm, and the mud with a certain thickness can enable parent loaches to inhabit well in the pool and accord with the ecological habit that the loaches like to drill the mud. The anti-escape net is arranged below the water line around the pond, the pond is provided with one water inlet and outlet, the water inlet is positioned at the upper end of the pond, the water outlet is arranged at the lowest position of the bottom of the pond, and the bottom of the pond is provided with 0.5% gradient from the water inlet end to the water outlet, so that the pond water is drained conveniently and loaches are caught.
Disinfecting a parent breeding pond by quicklime about 10 days before parent loaches are put into the breeding pond, wherein each 667m of parent breeding pond is disinfected by quicklime275kg of quicklime is added to kill harmful organisms. Draining the pond water after 3 days of disinfection, injecting new water, and filtering the water injection port by using 80-mesh silk cloth to prevent wild trash fish and enemy organisms from entering the parent breeding pond.
Second, intensified cultivation before cross breeding
Intensive culture is carried out on parent loaches according to the following method, loach baits are fed for 2 times every day in the early stage, about ten days, 8-9 am and 4-5 pm respectively, the feeding amount is 1.5-2.3% of the total weight of the loaches in the pond, and the loach baits in the pond are eaten completely within 1 hour.
At the later stage, minced fillet is added into loach bait, the minced fillet is formed by crushing and processing whole small rough fishes in fresh water, the adding amount of the minced fillet is 20-30 percent of the total weight of the loach bait, preferably 20 percent of the total weight of the loach bait, and loach aphrodisiac hormone-producing hormone analogue No. 3 (LRH-A) is added into the loach bait at the same time3) The addition amount is 0.08-0.12 microgram, preferably 0.12 microgram, LRH-A per kilogram of bait3The addition time of the feed is 5-6 days, so that the feed is not suitable to be added for too long time, otherwise, the gonads of the loaches are overgrown, and even premature delivery can be caused. In sunny days, the parent culture pond is flushed once every 5 days, the flushing time is 0.5 hour each time, the flushing amount is 3-5cm higher than the pond water level so as to promote the gonad development of the loaches, and the process is about half a month。
Third, the induced spawning, mating and spawning of the loaches
Preferably, when the water temperature reaches more than 18 ℃, the breeding work of the loaches can be carried out, and when the spawning induction injection time of the loaches is 2-5 pm, the loaches can be ensured to mate at night, the natural spawning habit of the loaches is met, and the mating quality is improved.
The oxytocin injection hormone is composed of chorionic gonadotropin (HCG), and luteinizing hormone analogue No. 3 (LRH-A)3) And Diosdone (DOM). Through multiple experiments, preferably, each kilogram of female loach is HCG177-190 international unit + LRH-A35.2-6 microgram + DOM 0.86-0.9 mg, more preferably HCG177 International Unit + LRH-A per kilogram female loach35.2 microgram + DOM 0.86 milligram, prepared by 0.65% normal saline, the dosage of male loach is reduced by half, and the prepared oxytocin can effectively reduce the cost and improve the oxytocic effect of the loach.
The hatching apparatus that mating, laying eggs stage adopted refers to fig. 1, and hatching apparatus is formed by processing pottery jar 1, and jar 1 bottom is made into the toper, and jar 1 below is provided with fixed baseplate 2, and the highly preferred 130cm of jar 1, the volume is 150L, sets up row seedling pipe 3 in the bottom, and its end is crooked downwards, and row seedling pipe 3 preferred length is 30cm, and the diameter is 25mm, is provided with control valve 4 on row seedling pipe 3 for the quantity of row seedling of control row seedling pipe 3. The egg laying mesh bag 5 is arranged on the jar 1, the edge of the upper opening of the egg laying mesh bag is slightly larger than the jar opening of the jar 1, a cover mesh is arranged on the upper opening of the egg laying mesh bag 5, the egg laying mesh bag 5 is also conical, is preferably made of polyvinyl chloride mesh sheet, the depth is 40cm, and the mesh size is 16 meshes/cm2(ii) a The hatching device is also provided with an air charging device and a water inlet pipe, wherein the air charging device comprises an air charging pipe 6, an air charging switch 7 on the air charging pipe 6 and an air charging stone 8 at the tail end of the air charging pipe 6, and the air charging pipe 6 extends into the bottom in the jar 1; the water inlet pipe comprises a water inlet pipe switch 9 and a stainless steel pipe 10 communicated with the bottom of the cylinder 1; the overflow pipe is arranged 15cm away from the upper opening of the cylinder 1 and comprises a plastic bent pipe 11 and a bolting silk net 12, the diameter of the plastic bent pipe 11 is 20cm, and the mesh size of the bolting silk net 12 is 60 meshes/cm2Each hatching tank is provided with 2-4 overflow pipes.
28 male loaches and 35 female loaches which are injected are placed in the egg laying mesh bag 5 for mating and laying, the proportion is lower than the mating proportion of more common male loaches in China, the prior art generally considers that the number of male loaches is less, the number of the male loaches is far greater than that of the female loaches under the condition of not influencing the fertility rate, and the cost is higher; the utility model discloses through the experiment discovery under the prerequisite that does not influence the embryonated egg quality, the proportion of male loach and female loach can be selected at 1:1.2-1.4, preferred 1:1.25, has saved the quantity of male loach, can further reduce the cost of hastening parturition, has improved the quantity of female loach, can improve the egg laying volume, increases the rate of emergence of unit water.
Preferably, the water in tank 1 should be oxygenated for more than 5 hours prior to mating and spawning to ensure adequate dissolved oxygen for fertilized egg embryo development.
Loach mating and oviposition can be gone on in the indoor of dark, reduces artificial interference as far as possible, prevents that the mating from receiving the influence to influence the fertilization effect of loach ovum.
Fourth, hatching of loach fertilized eggs
Loaches mate and lay eggs in the egg laying mesh bags 5, the produced fertilized eggs can directly fall into the jar 1 and are incubated in the jar 1, the strong inflation amount and the water changing amount are properly kept for the first two days during incubation, sufficient dissolved oxygen is ensured for the fertilized eggs to develop, and after the loaches come out of the membrane, the inflation switch 7 is adjusted to reduce the inflation amount so as to prevent the loaches from violently stirring up and down in the incubation jar and causing the death due to the large consumption of physical strength.
Fifthly: larval culture
And (3) on the third day after hatching, after the egg yolk of the loach seedlings is basically absorbed, feeding the loach seedlings with bait for 3-4 times every day, after feeding for at least three days, discharging the loach seedlings through a seedling discharging pipe 3 arranged at the bottom of the vat 1, opening a control valve 4 when the loach seedlings emerge, discharging the loach seedlings to a seedling collecting box through the seedling discharging pipe 3 bent downwards, fishing out and counting the loach seedlings in the seedling collecting box, transporting and selling the loach seedlings by using a nylon bag in an oxygenated mode, or directly putting the loach seedlings into a seedling culture pond, and entering a seedling culture stage.
As a result of the hybridization breeding method, 10-11 ten thousand loach larvae can be produced in each jar, and 100-110 ten thousand loach larvae in each batch. The production cost is reduced by about 55 percent compared with that of loach bred in an indoor greenhouse.
The utility model discloses loach mating breeding method has accomplished the mating and has laid eggs and the integration production of hatching, convenient operation, and the loach seed of breeding is good to external environment's adaptability, and the survival rate is high, has overcome the loach seed that the greenhouse was bred and has had the shortcoming poor to external environment adaptability. The breeding production links are reduced, the breeding cost of the loaches is further reduced, and the breeding benefit of the loaches is improved. Meanwhile, the selective crossbreeding problem of the loaches is solved, the loaches of different regions are selected for crossbreeding, and the loaches are produced in a single hatching jar in small and large batches, so that the first filial generation mixing caused by the large-scale breeding of the loaches of different varieties is avoided.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, many variations and modifications can be made without departing from the principle of the present invention, and this should be considered as the protection scope of the present invention.

Claims (4)

1. The utility model provides a loach embryonated egg hatching apparatus, hatching apparatus includes jar (1), aerating device and inlet tube, its characterized in that, jar (1) bottom sets up row seedling pipe (3), row seedling pipe (3) terminal downwarping be provided with control valve (4) on row seedling pipe (3), be equipped with in the jar mouth of jar (1) and lay eggs pocket (5), it is neighbouring the jar mouth outside of jar (1) is equipped with the warning pipe.
2. The hatching apparatus for loach fertilized eggs according to claim 1, wherein a cover net is provided at an upper opening of the egg-laying net bag (5), and the egg-laying net bag (5) is made of polyvinyl chloride.
3. An incubation device for loach zygotes according to claim 1 or 2, wherein the inflation device comprises an inflation tube (6) extending to the bottom of the jar (1), an inflation switch (7) provided on the inflation tube (6), and an inflation stone (8) at the end of the inflation tube (6).
4. The hatching device for loach fertilized eggs according to claim 1 or 2, wherein the number of the overflow pipes is 2-4, and the overflow pipes comprise a plastic elbow pipe (11) and a bolting silk net (12).
CN2012201179299U 2012-03-26 2012-03-26 Loach germ cell incubating device Expired - Fee Related CN202680227U (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102599091A (en) * 2012-03-26 2012-07-25 浙江海洋学院 Hybrid propagation method and fertilized ovum hatching device for loach
CN104381168A (en) * 2014-11-04 2015-03-04 广安市前锋区华瑞泥鳅养殖有限责任公司 Rapid propagation method for mud fish
CN107114280A (en) * 2017-06-19 2017-09-01 杭州富阳水灵哥生态农业开发有限公司 The method for culturing seedlings of loach

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102599091A (en) * 2012-03-26 2012-07-25 浙江海洋学院 Hybrid propagation method and fertilized ovum hatching device for loach
CN102599091B (en) * 2012-03-26 2014-12-24 浙江海洋学院 Hybrid propagation method
CN104381168A (en) * 2014-11-04 2015-03-04 广安市前锋区华瑞泥鳅养殖有限责任公司 Rapid propagation method for mud fish
CN107114280A (en) * 2017-06-19 2017-09-01 杭州富阳水灵哥生态农业开发有限公司 The method for culturing seedlings of loach

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Legal Events

Date Code Title Description
C14 Grant of patent or utility model
GR01 Patent grant
EE01 Entry into force of recordation of patent licensing contract

Assignee: Zhejiang aquatic ginseng Ecological Agriculture Development Co., Ltd.

Assignor: Zhejiang Ocean University

Contract record no.: 2014330000133

Denomination of utility model: Loach germ cell incubating device

Granted publication date: 20130123

License type: Exclusive License

Record date: 20140506

LICC Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20130123

Termination date: 20150326

EXPY Termination of patent right or utility model