CN114190338A - Indoor propagation method and application of Helicoverpa armigera Okayata - Google Patents
Indoor propagation method and application of Helicoverpa armigera Okayata Download PDFInfo
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- CN114190338A CN114190338A CN202210064692.0A CN202210064692A CN114190338A CN 114190338 A CN114190338 A CN 114190338A CN 202210064692 A CN202210064692 A CN 202210064692A CN 114190338 A CN114190338 A CN 114190338A
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- 241001147381 Helicoverpa armigera Species 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 20
- 241000255967 Helicoverpa zea Species 0.000 claims abstract description 33
- 238000009395 breeding Methods 0.000 claims abstract description 21
- 229930105110 Cyclosporin A Natural products 0.000 claims abstract description 20
- PMATZTZNYRCHOR-CGLBZJNRSA-N Cyclosporin A Chemical group CC[C@@H]1NC(=O)[C@H]([C@H](O)[C@H](C)C\C=C\C)N(C)C(=O)[C@H](C(C)C)N(C)C(=O)[C@H](CC(C)C)N(C)C(=O)[C@H](CC(C)C)N(C)C(=O)[C@@H](C)NC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)N(C)C(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)N(C)C(=O)CN(C)C1=O PMATZTZNYRCHOR-CGLBZJNRSA-N 0.000 claims abstract description 20
- 108010036949 Cyclosporine Proteins 0.000 claims abstract description 20
- 230000001488 breeding effect Effects 0.000 claims abstract description 17
- 229960001265 ciclosporin Drugs 0.000 claims description 6
- 241000409991 Mythimna separata Species 0.000 claims description 3
- 241000277334 Oncorhynchus Species 0.000 claims description 3
- 241001477931 Mythimna unipuncta Species 0.000 abstract description 27
- 230000003071 parasitic effect Effects 0.000 abstract description 10
- 230000024241 parasitism Effects 0.000 abstract description 10
- 241001124717 Ichneumon Species 0.000 abstract description 7
- 238000005516 engineering process Methods 0.000 abstract description 7
- 241000255777 Lepidoptera Species 0.000 abstract description 5
- 241001672662 Odontoglossum Species 0.000 abstract description 5
- 239000003814 drug Substances 0.000 abstract description 5
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- 241001494184 Myxozoa Species 0.000 description 5
- 241000257303 Hymenoptera Species 0.000 description 4
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
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- 235000013305 food Nutrition 0.000 description 2
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- 241000223782 Ciliophora Species 0.000 description 1
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- 102000002322 Egg Proteins Human genes 0.000 description 1
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- 241000283899 Gazella Species 0.000 description 1
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- 235000009432 Gossypium hirsutum Nutrition 0.000 description 1
- 241000255990 Helicoverpa Species 0.000 description 1
- 208000030852 Parasitic disease Diseases 0.000 description 1
- 241000256248 Spodoptera Species 0.000 description 1
- 241000256247 Spodoptera exigua Species 0.000 description 1
- 241000256251 Spodoptera frugiperda Species 0.000 description 1
- 241000223997 Toxoplasma gondii Species 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention belongs to the field of biological control, relates to a breeding technology of natural enemy insect Helicoverpa armigera, and particularly relates to an indoor breeding method and application of Helicoverpa armigera. The used medicament is cyclosporine A, the cocooning rate of the cotton bollworm tooth-lip ichneumon fly parasitic armyworms is improved by feeding the armyworm cyclosporine A, 2-year-old terminal armyworms with consistent growth are selected, the cotton bollworm tooth-lip ichneumon fly is parasitic after being fed with the cyclosporine A for one day, the cocooning rate of the cotton bollworm tooth-lip ichneumon fly is counted, CsA is taken for one day, and the cocooning rate of the cotton bollworm tooth-lip ichneumon fly is improved by 18.67%. Counting the emergence rate of cocoons, and finding that the emergence rate of the armyworms eating CsA for one day is 21.72 percent higher than that of the cocoons in the parasitism situation. The inventor finds that the bollworm odontoglossum bee is remarkably improved in cocooning rate and emergence rate after the armyworm eats the cyclosporine A, and the method has important significance for indoor breeding of the bollworm odontoglossum bee by utilizing the armyworm and preventing and controlling lepidoptera.
Description
Technical Field
The invention belongs to the field of biological control, relates to a breeding technology of natural enemy insect Helicoverpa armigera, and particularly relates to an indoor breeding method and application of Helicoverpa armigera.
Background
Lepidoptera pests, cotton bollworms, armyworms, spodoptera exigua, spodoptera frugiperda and the like are important agricultural pests which harm crops such as wheat, corn, cotton, vegetables and the like. At present, the prevention and control of the pests are mainly based on chemical prevention and control, and the problems of environment, food safety, human health and ecology and resistance caused by the chemical prevention and control are increasingly serious. Biological control technologies are receiving greater attention to better manage and control pests, and natural enemies of pests are the control means of priority due to the ability to specifically search for hosts and the safety and sustainability of ecosystems. The use of natural enemies for controlling pests is a control means which is considered as a priority in biological control at present and is forced by the increasing demands of people on ecological environment and food safety. The technical bottleneck of indoor large-scale breeding of the Helicoverpa armigera is solved, the Helicoverpa armigera is well applied to the control of the Lepidoptera insects, the requirements of improving the crop yield and the quality of crops are met, and the win-win of agricultural economic benefits and environmental benefits is realized.
Gossypium hirsutum (Linnaeus) ChingCampoletis chlorideaeUchida is an excellent natural enemy resource, is widely distributed in China, has strong adaptability, can parasitize more than 30 lepidoptera noctuid pests, including Spodoptera insects, and has excellent parasitic bee characteristics. The development of the indoor scale breeding technology of the Helicoverpa armigera Oncorhynchus in the room implements a sustainable control strategy of controlling insects by the insects, and is an effective means for controlling lepidoptera pests. However, under natural conditions, due to the lag and insufficient number of natural enemies, the rational release of parasitic wasps is an important biocontrol tool during the peak of pest occurrence. Therefore, cotton bollworm breeding work is required to be carried out indoors.
Although the sciaenopsis cincticeps can parasitize more than 30 lepidoptera noctuid pests, the sciaenopsis cincticeps prefers to parasitize 2-3-year-old cotton bollworm larvae, and the cotton bollworm larvae have the habit of killing each other, so that the indoor mass breeding of the cotton bollworms is a great problem. As a sub-dominant species, the Helicoverpa armigera has a certain parasitic effect on myxozoa, the myxozoa larvae do not have the habit of mutual killing, the indoor feeding technology is mature, and indoor large-scale breeding can be realized. Therefore, how to improve the cocooning rate of the cotton bollworm after the parasitizing and armyworm of the hypelitis chiselis is a technical bottleneck for breaking through indoor large-scale breeding. Over the past decade, researchers have made various attempts, including increasing cocooning rate by parasitizing armyworms as reported in 2010, but the parasitism itself is a waste of resources, and higher cocooning rate is achieved by allowing bees to parasitize armyworms many times. Actually, the cotton bollworm tooth-lipped ichneumon fly can produce one wasp by laying one ovum for 1 time, and the cotton bollworm tooth-lipped ichneumon fly can produce one wasp by laying a plurality of ova for a plurality of times through parasitism. In practice, this is a discounting operation for mass breeding with a limited number of hosts. Furthermore, the parasitism causes the competition of a plurality of bee eggs in the myxozoa, which results in the influence on the quality of the bees, including the size, the fecundity of offspring and the emergence rate.
Disclosure of Invention
The invention provides an indoor breeding method and application of Helicoverpa armigera, which solves the problem of low cocooning rate of Helicoverpa armigera on non-optimal host armyworms, increases the cocooning rate and improves the quantity of bred Helicoverpa armigera. The medicine related to the invention is CsA (CycyclosporineA) (CsA) with the molecular formula C62H111N11O12The relative molecular mass was 1202.61. According to the method, after 2-year-old terminal mythimna separata is fed with CsA of 30 mu M for one day, the Helicoverpa armigera is parasitized, the cocooning rate of the Helicoverpa armigera is counted, the cocooning rate of the Helicoverpa armigera parasitizing and feeding CsA is evaluated, the contribution of CsA treatment to improving the cocooning rate is determined, the cocooning rate of Helicoverpa armigera feeding is improved indoors, and a new method, an application method and effective concentration related to medicaments are provided for solving the problems of parasitic wasp breeding technology in biological control.
The technical scheme of the invention is realized as follows:
a method for indoor propagation of Helicoverpa armigera Oncorhynchus comprises the following steps:
(1) selecting myxoplasma with consistent growth to feed containing cyclosporine A, and obtaining a host of the treated Helicoverpa armigera Onck after one day;
(2) parasitizing the cotton bollworm tooth-shaped hemipilus on the host in the step (1) through spawning, and then transferring the host to a normal feed for feeding for indoor propagation.
Preferably, the myxoplasma in the step (1) is terminal myxoplasma of 2 years old.
Preferably, the formula of the cyclosporine A is C62H111N11O12The concentration of cyclosporin A in the feed was 30. mu.M.
Preferably, the period of feeding on normal feed in step (2) is 5-7 days.
The method is applied to large-scale breeding of the cotton bollworm tooth-labraska wasps.
The method is applied to improving the cocooning rate of the Helicoverpa armigera.
The invention has the following beneficial effects:
1. according to the method, after 2-year-old terminal armyworms are fed with 30 mu M cyclosporin A for one day, the cotton bollworm tooth-lipped ichneumonid is parasitized, the cocooning rate of the cotton bollworm tooth-lipped ichneumonid is counted, and the cocooning rate of the cotton bollworm tooth-lipped ichneumonid is improved by 18.67% on the CsA-fed armyworm for one day. Counting the emergence rate of cocoons, and finding that the emergence rate of the armyworms eating CsA for one day is 21.72 percent higher than that of the cocoons in the parasitism situation. The inventor finds that the bollworm odontoglossum bee eating cyclosporine A can obviously improve the cocooning rate and the emergence rate of the bollworm odontoglossum bee, and the application has important significance for utilizing the natural enemy to control lepidoptera by utilizing the indoor bred bollworm odontoglossum bee. Provides a new method and an application method thereof and relates to the effective concentration of medicaments for improving the cocooning rate of the cotton bollworm Oychia chirophus breeding indoors and solving the problem of the breeding technology of parasitic wasps in biological control.
2. According to the method, the cyclosporine A is utilized to establish the indoor bee breeding method and the usage and measurement of the cyclosporine A, the in-vivo defense of the armyworm to the cotton bollworm ichneumoniae is weakened, the technical problem of indoor mass breeding of the cotton bollworm ichneumoniae is solved, the commercialization of the cotton bollworm ichneumoniae is realized, and the cocooning rate of the cotton bollworm ichneumoniae is greatly improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 shows the cocooning rate of the armyworms at the terminal 2 nd age after parasitization by Helicoverpa armigera.
FIG. 2 shows the emergence rate of the parasitic armyworm cocoons of Chouioia ciliata.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without inventive effort based on the embodiments of the present invention, are within the scope of the present invention.
Example 1: the influence of cyclosporin A on the cocooning rate of Helicoverpa armigera after host treatment was investigated.
According to the method, after 2-year-old terminal armyworms are fed with 30 mu M of cyclosporine A for one day, the cotton bollworm Ongchira hirsuta is subjected to parasitism, the cocooning rate of the cotton bollworm Ochira hirsuta is counted, and the cocooning rate of the armyworms parasitized and fed with the cyclosporine A is evaluated.
1: preparing a feed containing 20 μ M of eco-factor A and a control sample (containing an equal volume of solvent DMSO in which CsA is dissolved);
2: armyworm larvae at the end of second instar were selected and fed with feed containing CsA concentration of 30. mu.M for 1 day (25 ℃ at room temperature). The myxozoa feeding control sample corresponding to the development state is set as a control group;
3; selecting 60 female bollworm tooth-lipped ichneumon fly bees with the same size.
4: myxoworm larvae were picked from each of the 2 groups for single-head parasitism, with 50 larvae per group, and repeated 3 times. (for data representation, each female bee is parasitic for no more than 10 armyworms, and the armyworms parasitic for each bee are treated and compared respectively)
5: and during the parasitism treatment, the myxozoa which are not eaten by CsA and correspond to the development state adopt multi-parasitism, and ensure that the parasitic wasp tail needle is successfully pricked for more than 2 times.
6: data arrangement: after the parasitism, counting the cocooning number of each group in 5-7 days, and calculating the cocooning rate of each group; and after 7 days, counting the emergence number of the cocoons in each group, and calculating the emergence rate of each group.
The experiment was repeated for 3 times.
Data analysis
Cocooning rate = (total cocooning number/total parasitic host insect number) × 100%.
Eclosion rate = (total number of bees/total number of cocoons) × 100%.
Differential analysis of cyclosporin a treated experimental and solvent treated control groups was performed using DPS version 9.01 software.
As shown in FIG. 1, it is understood from FIG. 1 that the callosity on the armyworm fed CsA for one day is increased by 18.67% and is equivalent to the callosity in the parasitosis (percentage of cocoons on the Chouioia gazelle)P= 0.0003). It is known that the rate of cocooning of the parasitized Cichorium coloratum feeding cyclosporine A increases significantly after the Toxoplasma gondii is picked (P=0.0003）。
From the emergence rates of cocoons in fig. 2, it was found that the emergence rate of the helicoverpa tooth-lipped ichneumoniae on the slime worms fed with CsA for one day was comparable to the control and was 21.72% higher than that of cocoons in the parasitism case (P = 0.0002). It is known that the emergence rate of the armyworm feeding cyclosporin A is higher than that of the parasitized armyworm by parasitizing the labyrinthine A in the labyrinthine.
Therefore, the armyworm disclosed by the invention can obviously improve the cocooning rate of the cotton bollworm tooth-lipped ichneumonid after eating the medicament CsA, and provides a technical means for breeding a large number of cotton bollworm tooth-lipped ichneumonid indoors by utilizing the habit that the armyworm can be massively propagated indoors.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (6)
1. A method for indoor propagation of Helicoverpa armigera Oncorhynchus is characterized by comprising the following steps:
(1) selecting myxoplasma with consistent growth to feed containing cyclosporine A, and obtaining a host of the treated Helicoverpa armigera Onck after one day;
(2) parasitizing the cotton bollworm tooth-shaped hemipilus on the host in the step (1) through spawning, and then transferring the host to a normal feed for feeding for indoor propagation.
2. The method for indoor propagation of Helicoverpa armigera Georgia according to claim 1, wherein: the mythimna separata in the step (1) is terminal mythimna separata of 2 years old.
3. The method for indoor propagation of Helicoverpa armigera Queensis according to claim 2, wherein: the molecular formula of the cyclosporine A is C62H111N11O12The concentration of cyclosporin A in the feed was 30. mu.M.
4. The method for indoor propagation of Helicoverpa armigera Georgia according to claim 1, wherein: the feeding time on the normal feed in the step (2) is 5-7 days.
5. The use of the method of any one of claims 1 to 4 for scale breeding of Helicoverpa armigera Lam.
6. Use of the method of any one of claims 1 to 4 for increasing the cocooning rate of Helicoverpa armigera.
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Citations (5)
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|---|---|---|---|---|
| WO2017129121A1 (en) * | 2016-01-26 | 2017-08-03 | 南开大学 | Thiadiazole oxime ether strobilurin derivatives, preparation method therefor and use thereof |
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| CN110637821A (en) * | 2019-10-29 | 2020-01-03 | 河南农业大学 | Novel medicine for preventing and treating armyworm and application method thereof |
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| CN111226872A (en) * | 2020-03-19 | 2020-06-05 | 湖南省林业科学院 | Method for breeding cotton bollworm tooth-lipped ichneumon fly in large quantity indoors |
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Patent Citations (5)
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|---|---|---|---|---|
| WO2017129121A1 (en) * | 2016-01-26 | 2017-08-03 | 南开大学 | Thiadiazole oxime ether strobilurin derivatives, preparation method therefor and use thereof |
| CN208657710U (en) * | 2018-06-08 | 2019-03-29 | 河南农业大学 | A kind of device detecting the parasitism performance of parasitic wasp |
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Non-Patent Citations (1)
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