CN114208781B - Method for inhibiting age of spodoptera frugiperda and application of method - Google Patents
Method for inhibiting age of spodoptera frugiperda and application of method Download PDFInfo
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Abstract
The invention belongs to the field of biological prevention and control of pests by using parasitic wasps, relates to prevention and control of spodoptera frugiperda, and particularly relates to a method for inhibiting the age of spodoptera frugiperda and application thereof. The method comprises the following steps: feeding Spodoptera frugiperda feed containing cyclosporine A on the first day of 3 years old for one day, then allowing the cotton bollworm Oichneumoniae to parasitize, and feeding normal feed. The inventor finds that the age of Spodoptera frugiperda can be remarkably inhibited after the Spodoptera frugiperda eats the cyclosporine A; after the Helicoverpa armigera is utilized, the cocooning rate of the Helicoverpa armigera is remarkably improved, and the Helicoverpa armigera has important significance for utilizing the Helicoverpa armigera to prevent and control Spodoptera frugiperda and realizing the long-term safety of Spodoptera frugiperda in fields.
Description
Technical Field
The invention belongs to the field of biological prevention and control of pests by using parasitic wasps, relates to prevention and control of spodoptera frugiperda, and particularly relates to a method for inhibiting the age of spodoptera frugiperda and application thereof.
Background
Spodoptera frugiperdaSpodoptera frugiperda(Smith) (Lepidoptera: spodoptera) is a serious disaster-causing pest invading China in 2019. At present, the insect invades 26 provinces in China, the diffusion speed is extremely high, and the harm area is large. Due to the typical polyphagia, the rice can eat more than 300 kinds of plants, particularly grain crops like preference corn, rice, wheat and the like, has strong reproductive capacity, causes great loss to Chinese agricultural production and is in an aggravated situation. At present, in addition to closely monitoring the migration route and the generation dynamics of Spodoptera frugiperda in China, how to effectively prevent and treat Spodoptera frugiperda becomes an urgent necessity for agricultural production. As an invasive species, the noctuid spodoptera frugiperda has remarkable characteristics that along with the migration of the noctuid spodoptera frugiperda, the noctuid spodoptera frugiperda can concentratedly lay eggs in a certain area and rapidly propagate, then the larvae hatch out after almost 3-5 days, and after about 1 week, the larvae enter the 4 th instar to begin to eat at a burst, which can cause huge economic loss in the area. Worse yet, these migratory intrusionsPests also have resistance to commonly used insecticides, and Spodoptera frugiperda has been reported to have resistance to chlorpyrifos, permethrin, lambda-cyhalothrin, and the like. In view of the harmful characteristics of Spodoptera frugiperda, effective measures for continuously preventing and controlling Spodoptera frugiperda need to be deeply explored and researched.
Gossypium hirsutum (Linnaeus) ChingCampoletischlorideaeUchida is used as an excellent natural enemy resource, is widely distributed in China, has strong adaptability, can parasitize more than 30 lepidoptera noctuid pests, including Spodoptera insects, and has excellent parasitic bee characteristics. Indoor researches in earlier stages find that the Helicoverpa armigera can successfully parasitize Spodoptera frugiperda larvae of 1-3 ages, preferably larvae of 2-3 ages. The Helicoverpa armigera has a parasitism rate and a cocooning rate of more than 80 percent on Spodoptera frugiperda of 2 years old. Such high parasitism and cocooning rates are due to indoor experiments, giving the bees enough time and space to have the opportunity to find the host and complete parasitism and cocooning. Along with the development of spodoptera frugiperda, especially after 3 ages later, although it still can be parasitized, because the internal rejection of host spodoptera frugiperda larva strengthens, can lead to cotton bollworm's lip ichneumoniae to face serious rejection and rejection response in spodoptera frugiperda 3 ages larva internal, it is difficult to accomplish growth and development and live and breed descendants.
In view of the characteristic of the spodoptera frugiperda to intensively burst, how to provide the cotton bollworm tooth-lipped ichneumonid with enough time to find a host and complete parasitism, and most importantly, breed more offspring to realize the continuity of prevention and control, and bring great challenges to biological prevention and control.
Disclosure of Invention
The invention provides a method for inhibiting the age of spodoptera frugiperda and application thereof, aiming at the problems of delaying the growth and development of spodoptera frugiperda and improving the cocooning rate of spodoptera frugiperda parasitized by Leptospira frugiperda, wherein the related medicament is Cyclosporine A, the English name is Cyclosporine A, the molecular formula is C 62 H 111 N 11 O 12 The relative molecular mass was 1202.61. The invention feeds Spodoptera frugiperda of the first day of 3 ages with 40 mu M cyclosporine A for one day, and then lets the cotton bollworm toothedThe parasitic Lysimachia clepias which eat the cyclosporine A are taken, the cocooning rate is counted, and the cocooning rate of the Spodoptera frugiperda which is parasitized and eaten with the cyclosporine A is evaluated. The effect and the dosage of the cyclosporine A for prolonging the development period of Spodoptera frugiperda are determined through experiments, the contribution to the improvement of the cocooning rate of the Helicoverpa armigera after the Spodoptera frugiperda which eats the cyclosporine A parasitizes is determined, the growth and development of invasive pests are delayed in the field, the selective parasitizing time of the parasitized bees is further enhanced, the cocooning rate is improved, and a potential biocontrol scheme is provided, so that a new method, a specific implementation scheme and effective concentration related to medicaments are provided for realizing the long-term control and the long-term safety of the Spodoptera frugiperda which invades the pests.
The technical scheme of the invention is realized as follows:
a method for inhibiting the age of Spodoptera frugiperda comprises the following steps:
1. preparing an environmental protection agent A feed with the concentration of 40 mu M and a control sample, feeding Spodoptera frugiperda of the first day of 3 ages for 1 day, and then turning to a normal feed; after 2 days, the age change of Spodoptera frugiperda was counted. Each treatment was performed in 24 aliquots and in 4 biological replicates.
2. Spodoptera frugiperda larvae of day 3 were selected and fed with a diet containing 40. Mu.M cyclosporin A for 1 day (room temperature, 25 ℃ C., same below). The normal feed of Spodoptera frugiperda in the corresponding development state was set as the control group.
3. 30 female bollworm Germinal-Goshiichia denticola bees with the same size were selected, and larvae were selected from each of the 2 groups for single-head parasitism (each treatment corresponds to a control of larvae of the same age), 24 larvae per group, and 4 replicates were performed.
4. After all the parasitism is completed, the parasitized spodoptera frugiperda is raised on a normal feed for about one week.
5. Data arrangement: counting the cocoons of each group, and calculating the cocoons rate of each group.
The invention has the following beneficial effects:
1. the method utilizes the cyclosporine A to delay the growth and development of spodoptera frugiperda on the grassland, on one hand, more time is given for pests to control before overeating, and more sufficient time is also given for the cotton bollworm Ongchira vittata to find a host for parasitizing, and meanwhile, the treatment obviously increases the survival rate (namely the cocooning rate) of the cotton bollworm Ongchira vittata after parasitizing the larvae at a higher development stage, so that the method has great promotion effect on the colonization of the cotton bollworm Ochikura vittata population in the natural world, and provides an ecological, green and continuous method scheme for realizing the control of the pests by the pests. Meanwhile, the propagation of more cotton bollworm tooth-lip ichneumoniae is promoted, the prevention and control of other lepidoptera insects are also beneficial, more virtuous cycles can be promoted, the requirements for improving the crop yield and the crop quality are met, and the win-win situation of agricultural economic benefits and environmental benefits is realized.
2. The Spodoptera frugiperda of 3 ages for 1 day is treated by 40 mu M of cyclosporine A, and then is fed with normal feed one day, so that 26% of Spodoptera frugiperda larvae are still in the third instar after 2 days. Meanwhile, after the parasitism of the hypsizigus marmoreus for the cotton bollworm takes the cyclosporine A for one day, the cocooning rate of the parasitism of the hypsizigus marmoreus for the cotton bollworm after the Spodoptera frugiperda is obviously improved. The inventor of the application finds that the spodoptera frugiperda can be obviously eaten after the spodoptera frugiperda gets the age of the spodoptera frugiperda, and the cocooning rate of the Spodoptera clogiperda is obviously improved, so that the spodoptera frugiperda is prevented and treated by the Spodoptera frugiperda and the long-lasting safety of the Spodoptera frugiperda in the field is realized.
3. The normal development of spodoptera frugiperda can be remarkably delayed by feeding the ciclosporin A for 1 day, so that parasitism of the cotton bollworm of the Choristoneura dentata is given for a longer time and with a higher probability (if the ciclosporin A is fed for a longer time, on one hand, the medicine is wasted, and on the other hand, the weight of the larva is remarkably reduced to influence the growth and development of the bees). In addition, the cocooning rate of the parasitized spodoptera frugiperda is counted, and the cocooning rate is improved by 13.788%.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 shows the development stage distribution of Spodoptera frugiperda larvae at the first day of three instars, which feed on cyclosporin A, 3 days later.
FIG. 2 shows the cocooning rate of Spodoptera frugiperda larvae feeding on cyclosporin A after parasitization by Helicoverpa armigera.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without inventive effort based on the embodiments of the present invention, are within the scope of the present invention.
The agent used in the present application is cyclosporin A, formula C 62 H 111 N 11 O 12 The relative molecular mass was 1202.61.
Example 1: explores the influence of cyclosporine A on the growth and development of Spodoptera frugiperda
The method for delaying the growth and development of spodoptera frugiperda comprises the following steps:
1. preparing cyclosporine A feed with the concentration of 40 mu M and a control sample;
2. spodoptera frugiperda larvae of day 3 were selected and fed with a diet containing 40. Mu.M cyclosporin A for 1 day (room temperature, 25 ℃ C., same below). The normal feed of Spodoptera frugiperda in the corresponding development state was set as the control group.
The development results are shown in FIG. 1, and it is understood from FIG. 1 that the development period of Spodoptera frugiperda larvae fed on cyclosporin A is significantly prolonged.
Example 2: effect of Spodoptera frugiperda treated in example 1 on the cocooning rate of Helicoverpa armigera
The method for improving the cocooning rate of the helicoverpa armigera on the spodoptera frugiperda comprises the following steps:
1. 30 female Heliothis virescens of the same size were selected and larvae were individually parasitized from each of the 2 groups and parasitized in Spodoptera frugiperda treated in example 1 (each treatment corresponds to a control of larvae of the same instar), 24 larvae per group, 4 replicates.
2. After all the parasites were completed, spodoptera frugiperda was raised on normal feed for approximately one week.
3. Data arrangement: counting the number of cocoons in each group, and calculating the cocooning rate of each group.
As shown in FIG. 2, it is understood from FIG. 2 that the spodoptera frugiperda, which is a species feeding on cyclosporin A, parasitized on Heliothis armigera labyrinthica, significantly increased the cocooning rate.
Performing an Effect analysis
The above experiments were repeated 4 times in total, and the data were analyzed as follows:
distribution of 4 instar larvae: (number of larvae entering 4 instars/total worms) × 100%.
Distribution of 3 instar larvae: (larvae still at 3 instars/total worm) x 100%.
Cocooning rate = (total cocooning number of bees/total number of parasitic hosts) × 100%.
Differential analysis of cyclosporin a treated experimental and solvent treated control groups was performed using DPS version 9.01 software.
The control effect is that after 3 days, the normal development of spodoptera frugiperda can be remarkably delayed by feeding 1 day of cyclosporin A, nearly 96.88% of control spodoptera frugiperda enters 4-year old, but 26% of spodoptera frugiperda larvae are treated for 3-year old in a treatment group, which gives the cotton bollworm Olacrima parasitism for a longer time and a higher probability (if the longer time of feeding the cyclosporin A, on one hand, the medicament is wasted, and on the other hand, the excessive inhibition can remarkably reduce the weight of the larvae to influence the growth and development of the bees). In addition, the cocooning rate of the parasitized spodoptera frugiperda is counted, and the cocooning rate is improved by 13.788%.
Therefore, after the Spodoptera frugiperda eats the drug cyclosporine A, the duration of the larva can be remarkably delayed, the cocooning rate of the Helicoverpa armigera Ching can be remarkably improved, and a technical means is provided for realizing the controllability and continuity of insect control by insects.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (4)
1. The application of utilizing Spodoptera frugiperda in the third instar to improve the parasitism rate of the Helicoverpa armigera Oncorhynchus, and is characterized in that the method for obtaining the Spodoptera frugiperda in the third instar comprises the following steps: feeding Spodoptera frugiperda containing cyclosporine A at the first day of three ages to ensure that the Spodoptera frugiperda is still at the third age, feeding normal feed, and collecting Spodoptera frugiperda at the third age within 3 days;
the concentration of the cyclosporine A in the feed is 40 mu M; the feed containing cyclosporin A was fed for 24 hours.
2. Use according to claim 1, characterized by the steps of: the Helicoverpa armigera is parasitized on Spodoptera frugiperda in the third age period, so as to achieve the purpose of improving the cocooning rate.
3. The application of three-instar spodoptera frugiperda in improving the cocooning rate of the Helicoverpa armigera Oncorhynchus, is characterized in that the three-instar spodoptera frugiperda is obtained by the following steps: feeding Spodoptera frugiperda of the first day of three ages with feed containing cyclosporine A to ensure that the Spodoptera frugiperda is still in the third age, feeding normal feed, and collecting Spodoptera frugiperda of the third age within 3 days;
the concentration of the cyclosporine A in the feed is 40 mu M; the time for feeding the feed containing cyclosporin A was 24 hours.
4. Use according to claim 3, characterized in that the steps are: the Helicoverpa armigera is parasitized on Spodoptera frugiperda in the third age period, so as to achieve the purpose of improving the cocooning rate.
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| ATE95193T1 (en) * | 1987-06-17 | 1993-10-15 | Sandoz Ag | CYCLOSPORINS AND THEIR USE AS MEDICINAL PRODUCTS. |
| US6608045B2 (en) * | 1998-03-27 | 2003-08-19 | Chong Kun Dang Corporation | Streptomyces sp producing tautomycetin and immunosuppressant comprising tautomycetin as active ingredient |
| CN110637821B (en) * | 2019-10-29 | 2021-01-26 | 河南农业大学 | Novel medicine for preventing and treating armyworm and application method thereof |
| CN110731340B (en) * | 2019-10-29 | 2020-10-27 | 河南农业大学 | Medicinal synergist for preventing and treating armyworm and use method thereof |
| CN110692641B (en) * | 2019-10-29 | 2020-10-02 | 河南农业大学 | Method for preventing and treating prodenia litura by using cyclosporine A |
| CN111345188A (en) * | 2020-03-30 | 2020-06-30 | 海南省农业科学院植物保护研究所 | Spodoptera frugiperda parasitic wasp propagation method |
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