CN105795168A - Artificial feed for bat moth larvae and preparation method thereof - Google Patents
Artificial feed for bat moth larvae and preparation method thereof Download PDFInfo
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- CN105795168A CN105795168A CN201610275395.5A CN201610275395A CN105795168A CN 105795168 A CN105795168 A CN 105795168A CN 201610275395 A CN201610275395 A CN 201610275395A CN 105795168 A CN105795168 A CN 105795168A
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Abstract
The invention provides artificial feed for bat moth larvae and a preparation method thereof. The feed is prepared from the following raw materials in parts by weight: 180 to 220 parts of wheat bran, 230 to 260 parts of fish meal, 40 to 60 parts of corn flour, 60 to 90 parts of soybean meal, 100 to 140 parts of serpentgrass powder, 110 to 140 parts of yeast powder, 15 to 25 parts of agar, 4 to 6 parts of ethylparaben and 400 to 600 parts of sterile water. An insect source is hepialus menyuanicus or hepialus armoricanus, and the artificial feed is successfully prepared by virtue of the feeding practice of larvae in four years and by virtue of continuous preparation, feeding, formula improvement and experience summarizing.
Description
Technical field
The invention belongs to insect artificial diet's preparation technique field, be specifically related to a kind of Hepialus armorieanus Oberthur larva artificial feed and preparation method thereof.
Background technology
Hepialus armorieanus Oberthur Hepialus belongs to Lepidoptera Hepialidae, is the host insect of Cordyceps, when the nearly aging time larva of Hepialus was infected by Cordyceps fungus before and after winter, becomes Bombyx Batryticatus, therefore named " winter worm " after mycelial growth full polypide；To early summer, Bombyx Batryticatus head grows tubular Stroma and bassets, therefore named " summer grass ".It is containing various active materials such as cordycepin, cordycepic acid and Cordyceps polysaccharides, has the several functions such as kidney tonifying tonifying the lung, regulation immunity and antitumor, progressively becomes domestic and international hot-sale products because of good drug efficacy, and supply falls short of demand for product, price is surging.But Cordyceps wild resource is extremely limited, and excessively excavated throughout the year, cause yield to reduce year by year, gradually cannot be met popular growing demand.Therefore, artificial culture will become Cordyceps industry development, protection of resources and an important trend of plateau eco-environment protection and approach.China launched the artificial culture of Cordyceps from 1979, and Hepialus armorieanus Oberthur larva large-scale breeding could be successfully significant to its artificial culture.
At present, domestic Duo Jia research institution can carry out the small-scale of Hepialus armorieanus Oberthur larva and raise, but grow due to Hepialus armorieanus Oberthur insect growth and growing environment is required harshness, in addition feedstuff is single, undernutrition, mature larvae pupates, deformity or death easily occur when sprouting wings, causing the female male imago of Hepialus armorieanus Oberthur that can not obtain or obtain to be difficult to mating behavior and cannot obtain stable worm source, raising scale is extremely limited.It is demonstrated experimentally that single feed feeds the life that can preferably maintain Hepialus armorieanus Oberthur larva, but larval growth is grown slowly；Compared with synthetic feedstuff, the healthy pupa of equal number to be obtained needs the mature larvae of several times, and larval feeding also reaches the identical weight more feedstuffs of needs, causes the serious wasting of resources.At present, market has no the ready-made special feed prepared by the trophic level of Hepialus armorieanus Oberthur larva, it is therefore desirable to work out a kind of can be with the man-made feeds of mass rearing Hepialus armorieanus Oberthur larva and compound method thereof.Worm source of the present invention is Hepialus minyuancus, and the larva through 4 years raises practice, constantly formula feed, feed larva, improved formulations and analysis and summary, successfully make man-made feeds.
Summary of the invention
The main object of the present invention is to provide man-made feeds and the compound method thereof of a kind of Hepialus armorieanus Oberthur larva, solves single feed and feeds the problem causing Hepialus armorieanus Oberthur Larval foods deficiency, large-scale breeding difficulty and copulation breeding difficult；Another object of the present invention is to improve Hepialus armorieanus Oberthur larval growth rates and the problem reducing larvae feeds waste.
For achieving the above object, the present invention adopts the following technical scheme that
A kind of Hepialus armorieanus Oberthur larva artificial feed, described feedstuff includes following weight portion meter raw material: Testa Tritici 180-220 part, fish flour 230-260 part, Semen Maydis powder 40-60 part, analysis for soybean powder 60-90 part, Polygonum viviparum L. powder 100-140 part, yeast powder 110-140 part, agar 15-25 part, ethyl hydroxybenzoate 4-6 part, sterilized water 400-600 part.
Preferably, described feedstuff includes following weight portion meter raw material: 200 parts of Testa Tritici, fish flour 245 parts, Semen Maydis powder 50 parts, analysis for soybean powder 75 parts, 120 parts of Polygonum viviparum L. powder, yeast powder 125 parts, 20 parts of agar, ethyl hydroxybenzoate 5 parts and sterilized water 500 parts.
Preferably, described feedstuff includes following weight portion meter raw material: 200 parts of Testa Tritici, fish flour 230 parts, Semen Maydis powder 60 parts, analysis for soybean powder 90 parts, 100 parts of Polygonum viviparum L. powder, yeast powder 110 parts, 20 parts of agar, ethyl hydroxybenzoate 5 parts and sterilized water 600 parts.
The preparation method of a kind of Hepialus armorieanus Oberthur larva artificial feed, described method includes the following:
(1) Testa Tritici, fish flour, Semen Maydis powder, analysis for soybean powder, Polygonum viviparum L. powder and yeast powder are put into container by formula weighing；
(2), after measuring sterilized water addition step (1) container, pastel is mixed into thoroughly；
(3) separately measuring sterilized water and pour in pot, after little fire slight fever, weighing agar, ethyl hydroxybenzoate are put in pot, and heating makes it pour pastel after dissolving into, use Glass rod whole process to stir, take out after boiling with soft fire system；
(4) after boiling completes, pouring the container sterilized after being cooled to 60 DEG C into, use feed granulator to be pressed into granule, after vacuum packaging, low temperature storage is standby.
Specifically include following steps:
(1) by Testa Tritici, fish flour, Semen Maydis powder, analysis for soybean powder, Polygonum viviparum L. powder and yeast powder after 121 degrees Celsius of sterilizings of high-pressure steam sterilizing pan, weigh respective material by formula and put into container；
(2), after measuring sterilized water 250-350 part addition container, Glass rod is used to mix into each composition thoroughly pastel；
(3) separately measure sterilized water 150-250 part and pour in pot, weigh agar after being heated to 60 DEG C, ethyl hydroxybenzoate is put in pot, adding high flame makes it pour pastel into after dissolving, Glass rod whole process is used to stir, little fire is turned after boiling in pot, temperature is maintained between 85-95 DEG C, and boiling was taken out after 15-20 minute；
(4) after boiling completes, pouring the container sterilized after being cooled to 60 DEG C into, use feed granulator to be pressed into 1-2g granule, after vacuum packaging, 8-12 DEG C of low temperature storage is standby.
It is an advantage of the current invention that: these formula man-made feeds are nutritious comprehensively, good feeding effect, can be effectively improved the development progress of larva, it is ensured that larva grows continuously and healthily, individual stalwartness.Man-made feeds preparation method is simple, and after making granule, evacuation packaging is beneficial to storage, low temperature storage feedstuff long shelf-life, and pellet conveniently feeds input, it is easy to application.
Detailed description of the invention
Below by specific embodiment, the method for the present invention is further described.
The preparation method of a kind of Hepialus armorieanus Oberthur larva artificial feed, specifically includes following steps:
(1) by Testa Tritici, fish flour, Semen Maydis powder, analysis for soybean powder, Polygonum viviparum L. powder and yeast powder after 121 degrees Celsius of sterilizings of high-pressure steam sterilizing pan, weigh respective material by formula and put into container；
(2), after measuring sterilized water 250-350 part addition container, Glass rod is used to mix into each composition thoroughly pastel；
(3) separately measure sterilized water 150-250 part and pour in pot, weigh agar after being heated to 60 DEG C, ethyl hydroxybenzoate is put in pot, adding high flame makes it pour pastel into after dissolving, Glass rod whole process is used to stir, little fire is turned after boiling in pot, temperature is maintained between 85-95 DEG C, and boiling was taken out after 15-20 minute；
(4) after boiling completes, pouring the container sterilized after being cooled to 60 DEG C into, use feed granulator to be pressed into 1-2g granule, after vacuum packaging, 8-12 DEG C of low temperature storage is standby.
Embodiment 1
Launch Hepialus armorieanus Oberthur larva indoor to raise on a small scale in low altitude area, place: Fujian Province's Ningde City, the time period: on February 19th, 2014 to May 19, Hepialus minyuancus 5 instar larvae launches to schedule to last the man-made feeds feeding experiment of 90d.Every each Ingredients Weight of 1200g feedstuff is: Testa Tritici 200g, fish flour 245g, Semen Maydis powder 50g, analysis for soybean powder 75g, Polygonum viviparum L. powder 120g, yeast powder 125g.After above each composition high pressure steam sterilization; weigh by formula ratio and be placed in container; pastel is mixed into thoroughly after adding 300g sterilized water; separately take agar 20g, ethyl hydroxybenzoate 5g and sterilized water 200g; pour into and pot is poured into after heating for dissolving pastel; the slowly boiling of the least fire; while stirring; take out after 20 minutes; pour the container sterilized after being cooled to 60 DEG C into, use feed granulator to be pressed into the granule of 1.5g after condensation, prepare natural feed (Radix Dauci Sativae, the Ipomoea batatas Lam.) small pieces of 1.5g simultaneously; evacuation is packed, and low temperature storage is standby.
Raising Vespertilio 5 instar larvae being respectively connected to hungry 48h in vessel, Ipomoea batatas Lam. is group 1, and Radix Dauci Sativae is group 2, man-made feeds are group 3, are repeated 4 times, every weekly check or change feedstuff 1 time, observe larva and 3 kinds of feedstuffs are taken food situation, measure its weightening finish and food ingestion after 7d and calculate its feed conversion rate.Subsequently, carry out observing and measure worm weight at 30d, 60d, 90d respectively, calculate larval growth speed.Data use spss22.0 software to carry out significance analysis after Excel software typing processes.
Table 1 Hepialus armorieanus Oberthur larva takes food situation to 3 kinds of feedstuffs
Note: represent that difference is not notable (p ＞ 0.05) with column data shoulder note same letter, different letter representation significant differences (p ＜ 0.05).
From table 1, in the case of larva weightening finish difference not notable (p ＜ 0.05), Hepialus armorieanus Oberthur larva is significantly lower than single natural feed (p ＜ 0.05) to the food ingestion of man-made feeds, and larva is optimal to the conversion ratio of man-made feeds.
The impact on Hepialus armorieanus Oberthur larva worm weight of the 23 kinds of feedstuffs of table
With column data shoulder note same letter, note: larva starting weight is 100mg, represents that difference is notable (p ＞ 0.05), different letter representation significant differences (p ＜ 0.05) are lower same.
The impact on Hepialus armorieanus Oberthur larval growth speed of the 33 kinds of feedstuffs of table
From table 2, table 3, the speed of growth and the larva worm weight average of organizing 3 larvas are better than organizing 1, organizing 2, organize 1, organize 2 larvas when entering 60d-90d, declining to some extent occurs in larval growth rates, it is substantially less than group 3(p ＜ 0.05), especially organize 1 larval weight to occur declining, therefore take food man-made feeds and can ensure that larva growth promoter continuously and healthily.Observing to May 19, the larva ontogeny of group 3 is healthy and strong, and part larva prepupa even pupates.There is slight drying shrinkage in the larva individuality of group 1, and mortality rate is higher, pupates without prepupa；Organize 2 larva polypides less, pupate without prepupa.
Embodiment 2
Launch Hepialus armorieanus Oberthur larva indoor to raise on a small scale in low altitude area, place: Fujian Province's Ningde City, the time period: on February 19th, 2014 to May 19, Hepialus minyuancus 5 instar larvae launches to schedule to last the man-made feeds feeding experiment of 90d.Every each Ingredients Weight of 1200g feedstuff is: Testa Tritici 200g, fish flour 230g, Semen Maydis powder 60g, analysis for soybean powder 90g, Polygonum viviparum L. powder 100g, yeast powder 110g.After above each composition high pressure steam sterilization; weigh by formula ratio and be placed in container; pastel is mixed into thoroughly after adding 350g sterilized water; separately take agar 20g, ethyl hydroxybenzoate 5g and sterilized water 250g; pour into and pot is poured into after heating for dissolving pastel stirring; the slowly boiling of the least fire; while stirring; take out after 15 minutes; pour the container sterilized after being cooled to 60 DEG C into, use feed granulator to be pressed into the granule of 1.0g after condensation, be prepared as natural feed (Radix Dauci Sativae, the Ipomoea batatas Lam.) small pieces of about 1.0g simultaneously; evacuation is packed, and 8-12 DEG C of low temperature storage is standby.
Being respectively connected to Vespertilio 5 instar larvae of hungry 48h in raising vessel, Ipomoea batatas Lam. is group 1, and Radix Dauci Sativae is group 2, man-made feeds are group 3, are repeated 4 times, every weekly check or change feedstuff 1 time, observe larva and 3 kinds of feedstuffs are taken food situation, measure weightening finish and food ingestion after 7d and calculate its feed conversion rate.Subsequently, carry out observing and measure larva worm at 30d, 60d, 90d respectively and weigh and calculate its growth rate.Data use spss22.0 software to carry out significance analysis after Excel software typing processes.
Table 1 Hepialus armorieanus Oberthur larva takes food situation to 3 kinds of feedstuffs
Note: represent that difference is not notable (p ＞ 0.05) with column data shoulder note same letter, different letter representation significant differences (p ＜ 0.05).
From table 1, in the case of larva weightening finish difference not notable (p ＜ 0.05), Hepialus armorieanus Oberthur larva is significantly lower than single natural feed (p ＜ 0.05) to the food ingestion of man-made feeds, but larva is optimal to the conversion ratio of man-made feeds.
The impact on Hepialus armorieanus Oberthur larva worm weight of the 23 kinds of feedstuffs of table
With column data shoulder note same letter, note: larva starting weight is 100mg, represents that difference is notable (p ＞ 0.05), different letter representation significant differences (p ＜ 0.05) are lower same.
The impact on Hepialus armorieanus Oberthur larval growth speed of the 33 kinds of feedstuffs of table
Result of the test is basically identical with embodiment 1, and it is more slightly lower than embodiment 1 that man-made feeds feed larva only worm weight and growth rate.
It should be noted that Hepialus armorieanus Oberthur larvae development goes through the phase up to 3-4 under natural conditions, use single feed indoor to feed and development duration can be foreshortened to 15-20 month, use synthetic feedstuff to feed, its development duration can be shortened 2-5 month again.Above-described embodiment illustrates, synthetic feedstuff is better than single feed to the feeding effect of Hepialus armorieanus Oberthur larva, can promote that Hepialus armorieanus Oberthur larval growth is grown, and shortens larva and goes through the phase, and larva polypide is healthy and strong, and percentage of pupation is improved, and saves feed cost.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent and modification, all should belong to the covering scope of the present invention.
Claims (6)
1. a Hepialus armorieanus Oberthur larva artificial feed, it is characterised in that: described feedstuff includes following weight portion meter raw material: Testa Tritici 180-220 part, fish flour 230-260 part, Semen Maydis powder 40-60 part, analysis for soybean powder 60-90 part, Polygonum viviparum L. powder 100-140 part, yeast powder 110-140 part, agar 15-25 part, ethyl hydroxybenzoate 4-6 part, sterilized water 400-600 part.
A kind of Hepialus armorieanus Oberthur larva artificial feed the most according to claim 1, it is characterised in that: described feedstuff includes following weight portion meter raw material: 200 parts of Testa Tritici, fish flour 245 parts, Semen Maydis powder 50 parts, analysis for soybean powder 75 parts, 120 parts of Polygonum viviparum L. powder, yeast powder 125 parts, 20 parts of agar, ethyl hydroxybenzoate 5 parts and sterilized water 500 parts.
A kind of Hepialus armorieanus Oberthur larva artificial feed the most according to claim 1, it is characterised in that: described feedstuff includes following weight portion meter raw material: 180 parts of Testa Tritici, fish flour 230 parts, Semen Maydis powder 40 parts, analysis for soybean powder 60 parts, 140 parts of Polygonum viviparum L. powder, yeast powder 110 parts, 20 parts of agar, ethyl hydroxybenzoate 5 parts and sterilized water 600 parts.
A kind of Hepialus armorieanus Oberthur larva artificial feed the most according to claim 1, it is characterised in that: described Hepialus armorieanus Oberthur includes Hepialus and Hepialus minyuancus.
The preparation method of a kind of Hepialus armorieanus Oberthur larva artificial feed the most as claimed in claim 1, it is characterised in that: described method includes the following:
(1) Testa Tritici, fish flour, Semen Maydis powder, analysis for soybean powder, Polygonum viviparum L. powder and yeast powder are put into container by formula weighing；
(2), after measuring sterilized water addition step (1) container, pastel is mixed into thoroughly；
(3) separately measuring sterilized water and pour in pot, after little fire slight fever, weighing agar, ethyl hydroxybenzoate are put in pot, and heating makes it pour pastel after dissolving into, use Glass rod whole process to stir, take out after boiling with soft fire system；
(4) after boiling completes, pouring the container sterilized after being cooled to 60 DEG C into, use feed granulator to be pressed into granule, after vacuum packaging, low temperature storage is standby.
The preparation method of a kind of Hepialus armorieanus Oberthur larva artificial feed the most according to claim 5, it is characterised in that: described method step is as follows:
(1) by Testa Tritici, fish flour, Semen Maydis powder, analysis for soybean powder, Polygonum viviparum L. powder and yeast powder after 121 degrees Celsius of sterilizings of high-pressure steam sterilizing pan, weigh respective material by formula and put into container；
(2), after measuring sterilized water 250-350 part addition container, Glass rod is used to mix into each composition thoroughly pastel；
(3) separately measure sterilized water 150-250 part and pour in pot, weigh agar after being heated to 60 DEG C, ethyl hydroxybenzoate is put in pot, adding high flame makes it pour pastel into after dissolving, Glass rod whole process is used to stir, little fire is turned after boiling in pot, temperature is maintained between 85-95 DEG C, and boiling was taken out after 15-20 minute；
(4) after boiling completes, pouring the container sterilized after being cooled to 60 DEG C into, use feed granulator to be pressed into 1-2g granule, after vacuum packaging, 8-12 DEG C of low temperature storage is standby.
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Cited By (4)
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| CN106538902A (en) * | 2016-10-25 | 2017-03-29 | 广东省生物资源应用研究所 | A kind of man-made feeds of Vespertilio moth larvae and preparation method thereof |
| CN107549120A (en) * | 2017-09-27 | 2018-01-09 | 湖南师范大学 | The rearing method and its larvae feeds formula of a kind of bat moth |
| CN109221826A (en) * | 2018-09-30 | 2019-01-18 | 重庆市中药研究院 | A kind of artificial formula forage of bat moth and preparation method thereof |
| CN115226811A (en) * | 2022-07-25 | 2022-10-25 | 重庆市中药研究院 | Feed preservative and application thereof in preparation of cordyceps sinensis larva feed |
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| CN103371295A (en) * | 2012-04-19 | 2013-10-30 | 薛刚 | Cordyceps sinensis and hepialus based feed coating material and preparation method thereof and coating feed |
| CN103782969A (en) * | 2014-03-03 | 2014-05-14 | 广西壮族自治区林业科学研究院 | Method for breeding trichogramma pintoi indoors and corcyra cephalonica raising fodder |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106538902A (en) * | 2016-10-25 | 2017-03-29 | 广东省生物资源应用研究所 | A kind of man-made feeds of Vespertilio moth larvae and preparation method thereof |
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| JP2018536394A (en) * | 2016-10-25 | 2018-12-13 | 広東省生物資源応用研究所Ｇｕａｎｇｄｏｎｇ Ｉｎｓｔｉｔｕｔｅ Ｏｆ Ａｐｐｌｉｅｄ Ｂｉｏｌｏｇｉｃａｌ Ｒｅｓｏｕｒｃｅｓ | Artificial feed for bat larvae and method for preparing the same |
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| US10681921B2 (en) * | 2016-10-25 | 2020-06-16 | Guangdong Institute Of Applied Biological Resources | Artificial diet for ghost moth larvae and preparation method thereof |
| CN107549120A (en) * | 2017-09-27 | 2018-01-09 | 湖南师范大学 | The rearing method and its larvae feeds formula of a kind of bat moth |
| CN109221826A (en) * | 2018-09-30 | 2019-01-18 | 重庆市中药研究院 | A kind of artificial formula forage of bat moth and preparation method thereof |
| CN115226811A (en) * | 2022-07-25 | 2022-10-25 | 重庆市中药研究院 | Feed preservative and application thereof in preparation of cordyceps sinensis larva feed |
| CN115226811B (en) * | 2022-07-25 | 2024-03-26 | 重庆市中药研究院 | Feed preservative and application thereof in preparation of cordyceps sinensis larva feed |
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Application publication date: 20160727 |