CN110742023B - Breeding device and breeding method for fruit fly pupa parasitic wasps - Google Patents
Breeding device and breeding method for fruit fly pupa parasitic wasps Download PDFInfo
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- CN110742023B CN110742023B CN201911023836.2A CN201911023836A CN110742023B CN 110742023 B CN110742023 B CN 110742023B CN 201911023836 A CN201911023836 A CN 201911023836A CN 110742023 B CN110742023 B CN 110742023B
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- 241000255588 Tephritidae Species 0.000 title claims abstract description 35
- 241001481304 Vespoidea Species 0.000 title claims abstract description 30
- 241000382353 Pupa Species 0.000 title claims abstract description 21
- 238000009395 breeding Methods 0.000 title description 7
- 230000001488 breeding effect Effects 0.000 title description 5
- 230000009194 climbing Effects 0.000 claims abstract description 33
- 241000238631 Hexapoda Species 0.000 claims abstract description 29
- 230000003071 parasitic effect Effects 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 8
- 241000257303 Hymenoptera Species 0.000 claims abstract description 4
- 230000007704 transition Effects 0.000 claims description 11
- 235000013305 food Nutrition 0.000 claims description 5
- 241000607479 Yersinia pestis Species 0.000 claims description 4
- 235000013399 edible fruits Nutrition 0.000 claims description 4
- 230000032669 eclosion Effects 0.000 claims description 3
- 241000256856 Vespidae Species 0.000 claims 2
- 230000000384 rearing effect Effects 0.000 claims 2
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 abstract description 12
- 241000255601 Drosophila melanogaster Species 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 3
- 230000006378 damage Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Biomedical Technology (AREA)
- Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Catching Or Destruction (AREA)
Abstract
A feeding device and a feeding method for fruit fly pupa parasitic wasps are used for conveniently feeding the fruit fly pupa parasitic wasps. The feeding device comprises a feeding bottle, and the opening of the feeding bottle is wrapped with gauze; the support comprises a bag support, an insect receiving disc and a climbing rod which are sequentially arranged from top to bottom, the bag support is of a hollow structure with an open top, a through hole is formed in the side wall of the bag support, the insect receiving disc is also of a hollow structure with an open top, the top of the insect receiving disc is fixedly contacted with the outer wall of the bag support, an outlet is formed in the side wall of the bag support, and the upper end of the climbing rod extends into the outlet and is fixedly connected with the inner wall of the outlet; the lower end of the climbing rod is contacted with the inner wall of the feeding bottle. According to the feeding device and the feeding method for the parasitic wasps of the drosophila pupae, provided by the invention, the cultivation of the drosophila third-instar larvae can be conveniently realized, and the third-instar larvae can climb to the inner wall of the feeding bottle along the bracket when growing into the drosophila pupae; parasitic bees can be thrown into the feeding bottle at the moment. The whole feeding device can be convenient for feeding parasitic wasps and is flexible in operation.
Description
Technical Field
The invention relates to the technical field of cultivation of fruit fly pupa parasitic wasps, in particular to a device and a method for breeding fruit fly pupa parasitic wasps.
Background
Fruit flies are a pest that can harm a variety of fruits, resulting in reduced yield, spoilage of the fruits. The drosophila pupae is a stage in the life history of drosophila, and the parasitic wasp imagoes can be bred by culturing the drosophila pupae parasitic wasps in the stage. The parasitic wasps lay eggs in the fruit fly pupae, the parasitic wasps breed by utilizing nutrient substances in the fruit fly pupae, the fruit fly pupae die, and finally the parasitic wasps are hatched.
Disclosure of Invention
The invention aims to provide a device and a method for raising fruit fly pupa parasitic wasps, which are used for facilitating the three-instar larvae of fruit flies to climb to the inner wall of a culture bottle and further facilitating the parasitic wasp thrown into the culture bottle to be parasitic.
The technical scheme adopted by the invention for solving the technical problems is as follows: fruit bat pupa parasitic wasp's device of raising, characterized by, it includes:
the feeding bottle is a transparent piece, and gauze is wrapped at the mouth of the feeding bottle;
the support comprises a bag support, an insect receiving disc and a climbing rod which are sequentially arranged from top to bottom, the bag support is of a hollow structure with an open top, the bag support is used for placing a culture bag, a through hole is formed in the side wall of the bag support, the insect receiving disc is also of a hollow structure with an open top, the top of the insect receiving disc is fixedly contacted with the outer wall of the bag support, an outlet is formed in the side wall of the insect receiving disc, and the upper end of the climbing rod extends into the outlet and is fixedly connected with the inner wall of the outlet; the climbing rod is an elastic rod, and the lower end of the climbing rod is in contact with the inner wall of the feeding bottle.
Furthermore, the bag support is of a hemispherical structure, and a handle is fixed at the top of the bag support.
Furthermore, a pair of hanging rods is fixed at the top of the support, an inner ring is arranged on the inner wall of the mouth of the feeding bottle, a notch is formed in the inner ring, and the upper end of each hanging rod is arranged in the notch.
Furthermore, a transition rod is fixed on the inner wall of the insect receiving disc, the upper end of the transition rod extends into the through hole, and the fruit fly pupae pass through the through hole, enter the insect receiving disc along the transition rod and then pass through the outlet to move to the climbing rod.
Furthermore, a vertical rod is installed on the insect receiving plate in a threaded mode, and a pull wire is tied between the lower end of the vertical rod and the climbing rod.
Furthermore, a fixed seat is fixed on the inner wall of the insect receiving plate, and the vertical rod is in threaded connection with the fixed seat.
The method for raising the fruit fly pupa parasitic wasps is characterized by comprising the following steps of:
(1) putting the fruit fly larva and food into culture bag;
(2) placing the bracket into the feeding bottle, ensuring the bracket to be fixed in the feeding bottle and ensuring the climbing rod on the bracket to be contacted with the inner wall of the feeding bottle;
(3) placing the culture bag into a bag support at the upper part of the bracket, then wrapping gauze on the mouth of the feeding bottle, fastening the mouth of the feeding bottle through a rubber band, and placing the feeding bottle in a room-temperature (about 25 ℃) environment for cultivation;
(4) when a large amount of fruit fly pupas are attached to the inner wall of the feeding bottle, the gauze is detached, parasitic wasps are put into the feeding bottle, and then the parasitic wasps are parasitic on the fruit fly pupas to grow and breed;
(5) after the fruit fly pupae on the inner wall of the feeding bottle are parasitized, taking out the parasitized bees in the feeding bottle;
(6) preparing a new feeding bottle and a new culture bag, and repeating the steps (1) - (5) for one to two times to cultivate the parasitic wasps in the eclosion state.
The invention has the beneficial effects that: according to the feeding device and the feeding method for the parasitic wasps of the drosophila pupae, provided by the invention, the cultivation of the drosophila third-instar larvae can be conveniently realized, and the third-instar larvae can climb to the inner wall of the feeding bottle along the bracket when growing into the drosophila pupae; at the moment, the development condition of the third-instar larvae can be conveniently observed, when a large amount of drosophila melanogaster pupae are attached to the inner wall of the feeding bottle, the third-instar larvae basically become drosophila melanogaster pupae, parasitic wasps can be put into the feeding bottle, and the parasitic wasps engulf the drosophila melanogaster pupae to grow; after the pupae of the fruit flies in the feeding bottle are used up, the parasitic wasps can be taken out and put into a new feeding bottle. The whole feeding device can be convenient for feeding parasitic wasps and is flexible in operation.
Drawings
FIG. 1 is a front view of the feeding device of the present invention;
FIG. 2 is a three-dimensional view of a feeding bottle;
FIG. 3 is a schematic view of the internal structure of the feeding bottle;
FIG. 4 is a cross-sectional view of a feeding bottle;
FIG. 5 is a front view of the bracket;
FIG. 6 is a cross-sectional view of a stent;
in the figure: the breeding device comprises a breeding bottle 1, gauze 11, rubber bands 12, a placing surface 13, an inner ring 2, a gap 21, a bag support 3, a through hole 31, an ear plate 32, a hanging rod 33, a handle 34, an insect receiving disc 35, a transition rod 351, a climbing rod 36, a pull wire 37, a vertical rod 38 and a fixed seat 39.
Detailed Description
As shown in fig. 1 to 6, the feeding device of the present invention mainly includes a feeding bottle 1, gauze 11, rubber band 12, inner ring 2 and a support, and the feeding device of the present invention will be described in detail below with reference to the accompanying drawings.
As shown in figures 1 and 2, the feeding bottle 1 is a transparent glass bottle, and when in use, the mouth of the feeding bottle is wrapped with gauze 11, and then the gauze is tied by a rubber band 12. A placing surface 13 is arranged on the outer wall of the feeding bottle, an obtuse included angle is formed between the placing surface and the lower surface of the feeding bottle, and the feeding bottle is in an upright state after the lower surface of the feeding bottle is contacted with the ground; after the placing surface is contacted with the ground, the feeding bottle is in an inclined state, and parasitic bees can be conveniently put into the feeding bottle at the moment.
As shown in figure 4, an inner ring 2 is arranged in the mouth of the feeding bottle, the inner ring is a circular transparent piece, and a notch 21 is arranged on the inner wall of the inner ring and used for fixing the support.
As shown in figure 3, a support is placed on the inner side of the feeding bottle, the support comprises a bag support 3, a hanging rod 33, an insect receiving disc 35, a transition rod 351, a climbing rod 36, a pull wire 37 and a vertical rod 38, the bag support 3 is of a hemispherical hollow structure, the upper surface of the bag support is a plane, the top of the bag support is open, and the bag support is used for placing a culture bag. And placing the third-instar larvae of the drosophila into the culture bag and enabling the third-instar larvae to grow into drosophila pupae by virtue of the food in the culture bag. The outer wall of the bag support is fully provided with through holes 31, the top of the bag support is fixed with a pair of ear plates 32 arranged left and right, and the ear plates are fixed with hanging rods 33 which are bent rods as shown in fig. 5 and 6, the hanging rods comprise two sections arranged at an obtuse included angle, and the lower ends of the hanging rods are fixedly connected with the ear plates. When the bag support is used, the upper end of the hanging rod is placed in the notch, and the bag support can be fixed at the moment. The handle 34 is fixed on the top of the bag support, and the handle is convenient for taking the bag support. An insect receiving disc 35 is arranged below the bag support, the insect receiving disc is of a hollow structure with an open top, the open end of the insect receiving disc is fixedly connected with the outer wall of the bag support, and the fruit fly pupae in the bag support can penetrate through the through holes and then enter the insect receiving disc. Be fixed with a plurality of transition poles 351 in the insect receiving disc, the upper end of transition pole stretch into in the through-hole and with through-hole inner wall fixed connection, the lower extreme of transition pole and the inner wall fixed connection of insect receiving disc are equipped with the export on the lateral wall of insect receiving disc, are fixed with a plurality of climbing poles 36 on the outer wall of insect receiving disc, and the climbing pole is the plastics pole, and the upper end of climbing pole is arranged in the export and is fixed with export inner wall contact. The climbing rod has elasticity, when the bag holds in the palm arrange in raise the bottle and the upper end of peg arrange the breach in, the lower extreme and the contact of raising the bottle inner wall of climbing rod under the effect of self elasticity, and have certain extrusion force between raise the bottle inner wall and the climbing rod. A fixed seat 39 is fixed on the inner wall of the insect receiving disc, a vertical rod 38 is installed on the fixed seat in a threaded manner, and the upper end of the vertical rod extends into the bag support. The lower end of the vertical rod passes through the side wall of the insect receiving disc and then extends downwards. The pull wire 37 is arranged between the lower end of the vertical rod and the corresponding climbing rod, and when the vertical rod is rotated, the pull wire can be driven to be wound at the lower end of the vertical rod or detached from the vertical rod. When the pull wire is continuously wound on the vertical rod, the lower end of the climbing rod can be pulled to be away from the inner wall of the feeding bottle; when the stay wire is continuously detached from the vertical rod, the lower end of the climbing rod is gradually close to the inner wall of the feeding bottle under the action of the self elastic force until the climbing rod is contacted with the inner wall of the feeding bottle.
When the bracket is used, the bracket is placed in a feeding bottle, then the third-instar larvae and food are placed in a culture bag, the culture bag is placed in a bag support, gauze is wrapped at the mouth of the feeding bottle, and finally the mouth of the feeding bottle is fastened through a rubber band. When the third-instar larvae grow into the fruit fly pupae, the fruit fly pupae can enter the insect receiving disc from the bag support and then climb to the inner wall of the feeding bottle along the climbing rod. The lower extreme shrink of pole is climbed in rotatory montant messenger, and the horizontal size of support diminishes and is less than the bottle mouth size of raising the bottle this moment, and then can be convenient put into the bottle of raising the support. And when taking out the support, at first rotatory montant makes climbing rod and feeding bottle inner wall separation, can avoid climbing the pole to cause the injury to the adnexed parasitic bee of feeding bottle inner wall this moment.
The method for raising the fruit fly pupa parasitic wasps comprises the following steps:
(1) putting the fruit fly larva and food into culture bag;
(2) placing the bracket into the feeding bottle, ensuring the bracket to be fixed in the feeding bottle and ensuring the climbing rod on the bracket to be contacted with the inner wall of the feeding bottle;
(3) placing the culture bag into a bag support at the upper part of the bracket, then wrapping gauze on the mouth of the feeding bottle, fastening the mouth of the feeding bottle through a rubber band, and placing the feeding bottle in a room-temperature (about 25 ℃) environment for cultivation;
(4) when a large amount of fruit fly pupas are attached to the inner wall of the feeding bottle, the gauze is detached, parasitic wasps are put into the feeding bottle, and then the parasitic wasps are parasitic on the fruit fly pupas to grow and breed;
(5) after the quantity of the drosophila pupae on the inner wall of the feeding bottle is less or disappears, taking out the parasitic wasps in the feeding bottle;
(6) preparing a new feeding bottle and a new culture bag, and repeating the steps (1) - (5) for one to two times to cultivate the parasitic wasps in the eclosion state.
Claims (4)
1. Fruit bat pupa parasitic wasp's device of raising, characterized by, it includes:
the feeding bottle is a transparent piece, and gauze is wrapped at the mouth of the feeding bottle;
the support comprises a bag support, an insect receiving disc and a climbing rod which are sequentially arranged from top to bottom, the bag support is of a hemispherical hollow structure with an open top, the bag support is used for placing a culture bag, a through hole is formed in the side wall of the bag support, and a handle is fixed at the top of the bag support; the pest receiving disc is also of a hollow structure with an open top, the top of the pest receiving disc is fixedly contacted with the outer wall of the bag support, an outlet is formed in the side wall of the pest receiving disc, and the upper end of the climbing rod extends into the outlet and is fixedly connected with the inner wall of the outlet; the climbing rod is an elastic rod, and the lower end of the climbing rod is in contact with the inner wall of the feeding bottle; a transition rod is fixed on the inner wall of the insect receiving disc, the upper end of the transition rod extends into the through hole, and the fruit fly pupae pass through the through hole, enter the insect receiving disc along the transition rod, then pass through the outlet and move to the climbing rod; a vertical rod is arranged on the insect receiving disc in a threaded manner, and a pull wire is tied between the lower end of the vertical rod and the climbing rod.
2. The device for raising fruit fly pupa parasitic wasp according to claim 1, wherein a pair of hanging rods are fixed on the top of the support, an inner ring is provided on the inner wall of the mouth of the feeding bottle, a notch is provided on the inner ring, and the upper end of the hanging rod is placed in the notch.
3. The device for raising fruit fly pupa parasitic wasp according to claim 1, wherein a fixing seat is fixed on the inner wall of the insect receiving plate, and the vertical rod is in threaded connection with the fixing seat.
4. The method of rearing the fruit fly pupa parasitoid wasps using the apparatus for rearing the fruit fly pupa parasitoid wasps according to claim 1, characterized by comprising the steps of:
(1) putting the fruit fly larva and food into culture bag;
(2) placing the bracket into the feeding bottle, ensuring the bracket to be fixed in the feeding bottle and ensuring the climbing rod on the bracket to be contacted with the inner wall of the feeding bottle;
(3) placing the culture bag into a bag support at the upper part of the bracket, then wrapping gauze on the mouth of the feeding bottle, fastening the mouth of the feeding bottle through a rubber band, and placing the feeding bottle in a room temperature environment of 25 ℃ for cultivation;
(4) when a large amount of fruit fly pupas are attached to the inner wall of the feeding bottle, the gauze is detached, parasitic wasps are put into the feeding bottle, and then the parasitic wasps are parasitic on the fruit fly pupas to grow and breed;
(5) after the fruit fly pupae on the inner wall of the feeding bottle are parasitized, taking out the parasitized bees in the feeding bottle;
(6) preparing a new feeding bottle and a new culture bag, and repeating the steps (1) - (5) for one to two times to cultivate the parasitic wasps in the eclosion state.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911023836.2A CN110742023B (en) | 2019-10-25 | 2019-10-25 | Breeding device and breeding method for fruit fly pupa parasitic wasps |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911023836.2A CN110742023B (en) | 2019-10-25 | 2019-10-25 | Breeding device and breeding method for fruit fly pupa parasitic wasps |
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| Publication Number | Publication Date |
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| CN110742023A CN110742023A (en) | 2020-02-04 |
| CN110742023B true CN110742023B (en) | 2021-11-26 |
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