CN105340764A - Manufacturing method for pollution-free ecological pig-raising fermentation bed - Google Patents
Manufacturing method for pollution-free ecological pig-raising fermentation bed Download PDFInfo
- Publication number
- CN105340764A CN105340764A CN201410414866.7A CN201410414866A CN105340764A CN 105340764 A CN105340764 A CN 105340764A CN 201410414866 A CN201410414866 A CN 201410414866A CN 105340764 A CN105340764 A CN 105340764A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- fermentation bed
- organic
- manufacturing
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Abstract
The invention relates to a manufacturing method for a pollution-free ecological pig-raising fermentation bed. The manufacturing method is characterized by comprising preparation of a fermentation agent and manufacturing of the fermentation bed. The manufacturing method comprises the steps of: preparing the fermentation agent by combining saccharomycetes, lactic acid bacteria, actinomycetes, photosynthetic bacteria and filamentous fungi in proportion; uniformly stirring the activated fermentation agent with organic packing in proportion; and stacking and fermenting the mixture to prepare the fermentation bed to raise live pigs. The manufacturing method provided by the invention solves the problem that in an existing ecological pig raising method, the starting fermentation time of the organic packing is long, an inoculant is not stable, requirements on fermentation conditions and raw materials are strict and the service life is short. By using a compound culture fermentation agent to construct an ecological colony house, the starting fermentation time of the organic packing is short (2-3 days in summer and 3-5 days in winter), the culture is high in activity (the degradation process can be completed within 3-5 days) and the efficacy exertion is lasting (the fermentation bed can be continuously used for 6-10 years), and is green and environmental-friendly.
Description
Technical field
The present invention relates to pig-breeding technical field, specifically a kind of preparation method of nuisanceless ecologically raising pigs fermentation bed.
Background technology
Ecologically raising pigs method selects multiple-microorganism, is mixed to form organic bedding and padding, the functional microorganism degradable swine excrement in organic bedding and padding with a certain amount of rice husk, sawdust, auxiliary material etc.At present, the method mainly indigneous flora fermentation method that ecologically raising pigs method adopts, namely gathers microorganism, then the cultivation that spreads cultivation from natural environment soil, to be enriched in bedding and padding by the microorganism of collection to carry out fermentative degradation ight soil by traditional means.But it is lack of standardization that the shortcoming of this method is preparation method, and operating process is loaded down with trivial details, and the kind of microorganism and quantity are all wayward, and quality is extremely unstable, easy infection miscellaneous bacteria, and organic pad material fermentation time is long, and about 2 time-of-weeks, are not suitable for large-scale promotion.Although also there are some manufacturers to sell fermenting agent on the market, because bacterial classification used is to its growth and breeding environment and all require higher to the raw material of organic bedding and padding, and bacterial classification useful life is also shorter, is also subject to a definite limitation so promote.
Summary of the invention
The organic bedding and padding startup fermentation time that the object of the invention is to solve in existing ecologically raising pigs method is long, manufacturing process complex operation, microbial inoculum quality is unstable, bacterium number is wayward, flora is not easily determined, easy infection miscellaneous bacteria, requires strict and the short-life problem of bacterial classification to raw material and fermentation condition, provides a kind of preparation method of multiple nuisanceless ecologically raising pigs fermentation bed.
Inventor is by a large amount of tests, find that the synergy after following microorganism is carried out rational proportion has good ferment effect to live pig excreta, it is short that organic bedding and padding start fast fermentation time, the live pig excreta resolving time shortens greatly, and to the strong adaptability of fermentation condition, growing environment requires extensive, and it is lasting that bacterial classification effect plays the time.Composite bacteria leaven of the present invention is made primarily of saccharomycete, lactic acid bacteria, actinomycetes, photosynthetic bacteria and filamentous fungi;
Bacterial classification used is known bacterial classification, and concrete source is as follows:
Candida utili (CandidaunitsLodderetKueger) Kyoto Univ Japan finds, China Committee for Culture Collection of Microorganisms's preservation, preserving number is CGMCC, N02.1495.Fen Suo Dalian, Dalian, the inferior Dbaly yeast of the Chinese (Debaryomyceshansenii) northeast scientific research institution Y38, preserving number is CGMCC, raw institute is planted in Shanghai by N02.33 geotrichum candidum (Geolrichumcandidium) Chinese Academy of Sciences, preserving number is CGMCC, raw institute 372-24D plants in Shanghai in N02.498 Cellumomonas flavigena (Cellulomonasflavigena) Chinese Academy of Sciences, preserving number is CGMCC, Institute of Micro-biology of N01.1002 Lactobacillus plantarum (Lactobacillusplantarum) Chinese Academy of Sciences W46, preserving number is CGMCC, N01.556 pool red pseudomonas (Rhodopseudomonaspalustris) the Chinese Academy of Agricultural Sciences money draws institute 981, preserving number is ACCC, Institute of Micro-biology of N010650 Trichoderma viride (TrichodermaviridePers) Chinese Academy of Sciences, preserving number is CGMCC, N03.3711 aspergillus niger (Aspergillusniger) Huanghai Sea chemical industry research society Huanghai Sea 316, preserving number is CGMCC, the preservation of N03.316 bafillus natto (Bacillusnatto) Chinese agriculture Microbiological Culture Collection administrative center, preserving number is ACCC, N01III1, the preparation method of nuisanceless ecologically raising pigs fermentation bed is described below:
1. composite bacteria leaven preparation method
Slant strains cultivation → liquid first order seed (shaking flask) cultivation → liquid two stage (seeding tank) → fermentation tank culture → supercentrifuge → vacuum freeze drier → cracker → Counting alive microbial → bacterial classification combines → packs → finished product leavening seed culture in proportion: utilize any one in above-mentioned bacterial classification, conventional experiment condition is adopted to cultivate, namely with carbohydrate goods and materials, for carbon source, (said glucide can be glucose, sucrose, lactose, fructose, maltose, one or more in starch), with peptone, yeast extract or beef leaching thing are nitrogenous source, and add appropriate mineral salt and trace element, solution is deployed into as seed culture medium by deionized water, liquid bacteria a billion is obtained through cultivating, stand-by.Because the incubation of above bacterial classification is all with reference to cultural method described in Chinese bacterial classification catalogue, be all conventional laboratory facilities, therefore describe and conform to the principle of simplicity.
Slant strains is cultivated: each bacterial classification uses corresponding medium, inoculum concentration 5-10%, static gas wave refrigerator 20-48 hour under 28-350C condition, puts into 4 DEG C of Refrigerator stores.
Wherein shake flask culture conditions: temperature 30-40 DEG C, rotating speed 100-200 beat/min, inoculum concentration 2-4%, time 20-35 hour.
Fermentation tank culture condition: temperature 30-35 DEG C, ventilation 10-13 square metre/h, tank pressure 0.05-0.10Mpa, inoculum concentration 3-10%, time 24-36 hour.
Bacterial classification combines in proportion: qualified for mensuration bacterium number candida utili 10-20%, the inferior Dbaly yeast 10-15% of the Chinese, geotrichum candidum 10-15%, Cellumomonas flavigena 7-10%, Lactobacillus plantarum 2-5%, Rhodopseudomonas palustris 2-5%, Trichoderma viride 8-12%, aspergillus niger 6-10%, bafillus natto 12-17% are carried out combination and make leavening.
Composite bacteria leaven has following proterties:
Pale powder, tasteless or slightly tart flavour, viable count reaches 2.2xl0
11individual/g, moisture is below 10%.
2. the activation of composite bacteria leaven
Get corn flour 35-80%, thin chaff 35-80% mixes, leavening inoculum concentration 3-8 ‰, and amount of water 50-60%, mixes thoroughly three, and under being placed on 25-40 DEG C of condition, cultivate 15-20 hour, centre turns 2 times, keeps product temperature 25-40 DEG C.Cultivation instrument can in basin, in plastic sack, heat and moisture preserving.When cultivation charge level turns white, mycelia is gathered, and has sour-sweet fragrance to apply.
3. the fermentation of organic bedding and padding
Organic bedding and padding can be one or more the mixtures in sawdust, rice husk, straw powder etc., and the leavening after activation is pressed 8-l2kg/m
3the inoculation of organic bedding and padding, the 35-40% that adds water puddles evenly, heat and moisture preserving heap fermentation, and ferment summer 2-3 days, winter 3-5 days, organic bedding and padding central temperature reaches 30-35 DEG C, is fermentation and completes.
Described organic bedding and padding are preferably made up of the wood chip of 40-60% percent by volume, the rice husk of 30-50% percent by volume.
4. the making of ecologically raising pigs fermentation bed
Fermentation bed in colony house can selectively above formula and underground type according to subterranean water level, the degree of depth will ensure between 0.6-01.2m, bottom first can lay the thick agricultural crop straw (one or more mixtures in maize straw corncob, peanut seedling, Cotton Stalk, wheat straw etc.) that dries of 0.2-0.5m or juggle as loose ventilation bottom, lays the organic fermentation bedding and padding after 0.3-0.5m above; Also can lay loose ventilation bottom in advance, directly all lay organic fermentation bedding and padding, fermentation bed has been laid live pig to be caught up and has been raised.
Composite bacteria leaven of the present invention is as organic pad material fermentation agent.
Composite bacteria leaven of the present invention is used for ecological method and raises pigs.
The present invention utilizes making fermenting bed for ecological pig breeding by using composite strain culture to be raised pigs pattern as a kind of environment protection ecological type that energy cycle of matter, conversion " maincenter " have been used for by multiple-microorganism.Organic bedding and padding in ecological colony house, by the fermentation of functional microorganism, to meet in house live pig automatically to insulation, ventilation and to micro-physiological demand.In ecological colony house, live pig just lives on this organic bedding and padding from birth, pig and bedding and padding, Pig dung and urine, microorganism species is formed one " ecological chain ", fermentation bed resembles an Application of Artificial Intelligence, it is always in ceaselessly continuous productive process, bedding and padding, the organic matters such as Pig dung and urine are transformed by this " maincenter " recycle of powerful microorganism species in fermentation bed, microorganism is at " eating " bedding and padding, Pig dung and urine, pig (comprises various bacterium protein in " eating " microorganism, the multiple organic acid secreted in microorganism growth process, biological sugar, vitamin and hundreds of biology enzyme), the noresidue of whole pig house no waste mine, produce without soiled litter, so very health is clean in ecological colony house, be difficult to see fly, be as good as stink, not only be beneficial to the digestion of animal and improve immunity, feed conversion rate can also be made to improve, input-output ratio and feedstuff-meat ratio reduce.
Compared with prior art, method for culturing pigs of the present invention has following beneficial effect:
1. composite bacteria leaven of the present invention joins according to scientific formula essence according to live pig physiological property to form, collaboratively between multiple-microorganism flora to go forward one by one, have complementary functions and enzyme system complementary, give full play to the advantage of each probiotics strain, bacterial activity is strong, bacterium number is stablized, improve degradation efficiency, shorten the degradation cycle to swine excrement, within 2-4 days, can degradation process be completed.And during the fermentation of fermentation bed inside center, temperature can reach 60-70 DEG C, can kill the worm's ovum in ight soil and germ, so very health is clean in ecological colony house, be difficult to see fly, with fresh air, free from extraneous odour.
2. the bacterial strain of cellulase-producing used, hemicellulase, protease, amylase, pectase, tannase in composite bacteria leaven of the present invention, by the above-mentioned enzyme material secreted in its process of growth, by the raw fiber in organic bedding and padding, lignin, long strand decomposed, nonabsorbable for live pig polymeric carbohydrate is changed into the low molecular weight carbohydrate of Absorbable rod utilization, and enter by the arch food of pig the digestion that digestive system assists livestock endogenous enzymes, improve digesting and assimilating of feed, improve the stink of swine excrement.
3. acid-producing bacteria used in composite bacteria leaven of the present invention, can produce a large amount of organic acid (citric acid, malic acid, gluconic acid, acetic acid, lactic acid) and active carbohydrate material (glucose, maltose, trehalose).Organic acid can reduce the pH value of organic bedding and padding, suppresses the field planting of various putrefying bacteria to grow.Eat by the arch of pig the liquid secretion that can stimulate digestion, increase feed intake, promote gastrointestinal peristalsis, slow down gastrointestinal emptying speed, make nutriment have digestion time more fully, improve digestibility.Micro-ecological environment in livestock body can be improved simultaneously, suppress the growth of pathogenic bacteria in livestock body, improve the resistance against diseases of livestock.
4. the composite bacteria leaven in the present invention is multiple functional, and sphere of action is wide.Can be fermented various types of organic bedding and padding (sawdust, wood chip, rice husk, all kinds of agricultural crop straw, industrial dreg), and composite bacteria leaven is to the strong adaptability of fermentation condition, growth requires more extensive, temperature is between 10-30 DEG C, and water content, at 40-60%, all can be fermented.The fermentation bed safety non-toxic that composite bacteria leaven makes, and there is the effect of enhancing live pig premunition.
5. the present invention combines microbial inoculum in making ecologically raising pigs fermentation bed process, starts fast, persistent.It is short that organic bedding and padding start fast fermentation time, and heat production is fast, and method of operating is simple.Through inventor's many experiments, summer 2-3 days, 3-5 days winter can ferment salty, live pig can be driven in and feed.It is lasting that bacterial classification effect plays the time, and keeper carries out feeding and management, and ferment bed can use 6-10 continuously, environmental protection, can Long-Time Service.
Embodiment
1. composite bacteria leaven preparation method
Slant strains cultivation → liquid first order seed (shaking flask) cultivation → liquid two stage (seeding tank) → fermentation tank culture → supercentrifuge → vacuum freeze drier → cracker → Counting alive microbial → bacterial classification combines → packs → finished product leavening in proportion.
Seed culture: utilize any one in above-mentioned bacterial classification, conventional experiment condition is adopted to cultivate, namely with carbohydrate goods and materials for carbon source, with peptone, yeast extract or beef leaching thing for nitrogenous source, and add appropriate mineral salt and trace element, being deployed into solution as seed culture medium by deionized water, obtaining liquid spawn through cultivating, stand-by.Because the incubation of above bacterial classification is all with reference to cultural method described in Chinese bacterial classification catalogue, be all conventional laboratory facilities, therefore describe and conform to the principle of simplicity.
Slant strains is cultivated: each bacterial classification uses corresponding medium, inoculum concentration 6-12%, static gas wave refrigerator 24-48 hour under 30-35 DEG C of condition, puts into 4 DEG C of Refrigerator stores.
Wherein shake flask culture conditions: temperature 30-40 DEG C, rotating speed 100-200 beat/min, inoculum concentration 2-4%, time 20-35 hour.
Fermentation tank culture condition: temperature 30-35 DEG C, ventilation 10-13 square metre/h, tank pressure 0.05-0.10Mpa, inoculum concentration 3-10%, time 24-36 hour.
Bacterial classification combines in proportion: candida utili 20% qualified for mensuration bacterium number, the inferior Dbaly yeast 18% of the Chinese, geotrichum candidum 5%, Cellumomonas flavigena 10%, Lactobacillus plantarum 3%, Rhodopseudomonas palustris 5%, Trichoderma viride 8%, aspergillus niger 10%, bafillus natto 11% are carried out combination and make leavening.
Composite bacteria leaven has following proterties:
Pale powder, tasteless or slightly tart flavour, viable count reaches 2.2x10
llindividual/g, moisture is below 10%.
2. the activation of composite bacteria leaven
Get corn flour 30%, wheat bran or thin chaff 70% mix, composite bacteria leaven inoculum concentration 3 ‰, and amount of water 45-50%, mixes thoroughly three, and under being placed on 20-35 DEG C of condition, cultivate 12-18 hour, centre turns 2 times, keeps product temperature 20-35 DEG C.Cultivation instrument can in basin, in plastic sack, heat and moisture preserving.When cultivation charge level turns white, mycelia is gathered, and has sour-sweet fragrance to apply.
3. the fermentation of organic bedding and padding
Organic bedding and padding are preferably made up of the wood chip of 50% percent by volume and the rice husk of 50% percent by volume.Composite bacteria leaven after activation is pressed lOkg/m
3the inoculation of organic bedding and padding, the 40-45% that adds water puddles evenly, heat and moisture preserving heap fermentation, and ferment summer 1-2 days, winter 3-5 days, organic bedding and padding central temperature reaches 35-40 DEG C, is fermentation and completes.
4. the making of ecologically raising pigs fermentation bed
Fermentation bed in colony house can selectively above formula and underground type according to subterranean water level, the degree of depth will ensure between 0.8-1.2m, bottom lays the mixed house thing of the thick maize straw of 0.3-0.6m and corncob in advance as loose ventilation bottom, lays the organic fermentation bedding and padding after 0.4-0.8m above.Fermentation bed has been laid live pig to be caught up and has been raised.
Embodiment two
1. each method of composite bacteria leaven system
Slant strains cultivation → liquid first order seed (shaking flask) cultivation → liquid two stage (seeding tank) → fermentation tank culture → supercentrifuge → vacuum freeze drier → cracker → Counting alive microbial → bacterial classification combines → packs → finished product leavening in proportion.
Seed culture: utilize any one in above-mentioned bacterial classification, conventional experiment condition is adopted to cultivate, namely with carbohydrate goods and materials for carbon source (said glucide can be in glucose, sucrose, lactose, fructose, maltose, starch one or more), with peptone, yeast extract or beef leaching thing for nitrogenous source, and add appropriate mineral salt and trace element, being deployed into solution as seed culture medium by deionized water, obtaining liquid spawn through cultivating, stand-by.Because the incubation of above bacterial classification is all with reference to cultural method described in Chinese bacterial classification catalogue, be all conventional laboratory facilities, therefore describe and conform to the principle of simplicity.
Slant strains is cultivated: each bacterial classification uses corresponding medium, inoculum concentration 6-12%, static gas wave refrigerator 24-48 hour under 30-35 DEG C of condition, puts into 4 DEG C of Refrigerator stores.
Wherein shake flask culture conditions: temperature 30-40 DEG C, rotating speed 100-200 beat/min, inoculum concentration 2-4%, time 20-35 hour.
Fermentation tank culture condition: temperature 30-35 DEG C, ventilation 10-13 square metre/h, tank pressure 0.05-0.10Mpa, inoculum concentration 3-10%, time 24-36 hour.
Bacterial classification combines in proportion: candida utili 25% qualified for mensuration bacterium number, the inferior Dbaly yeast 15% of the Chinese, geotrichum candidum 12%, Cellumomonas flavigena 8%, Lactobacillus plantarum 3%, Rhodopseudomonas palustris 3%, Trichoderma viride l0%, aspergillus niger g%, bafillus natto 15% are carried out combination and make leavening.
Composite bacteria leaven has following proterties:
Pale powder, tasteless or slightly tart flavour, viable count reaches 2.2xl0
11individual/g, moisture is below 10%.
2. the activation of composite bacteria leaven
Get corn flour 45%, thin chaff 45% mixes, composite bacteria leaven inoculum concentration 5 ‰, and amount of water 45-50%, mixes thoroughly three, and under being placed on 20-35 DEG C of condition, cultivate 12-18 hour, centre turns 2 times, keeps product temperature 20-35 DEG C.Cultivation instrument can in basin, in plastic sack, heat and moisture preserving.When cultivation charge level turns white, mycelia is gathered, and has sour-sweet fragrance to apply.
3. the fermentation of organic bedding and padding
Organic bedding and padding are preferably made up of the wood chip of 50% percent by volume and the rice husk of 50% percent by volume.Composite bacteria leaven after activation is pressed lOkg/m
3the inoculation of organic bedding and padding, the 40-45% that adds water puddles evenly, heat and moisture preserving heap fermentation, and ferment summer 1-2 days, winter 3-5 days, organic bedding and padding central temperature reaches 35-40 DEG C, is fermentation and completes.
4. the making of ecologically raising pigs fermentation bed
Under fermentation bed base area in colony house, position can selectively above formula and underground type forever, the degree of depth will ensure between 0.8-1.2m, bottom lays the mixture of the thick Cotton Stalk of 0.3-0.6m and peanut seedling in advance as loose ventilation bottom, lays the organic fermentation bedding and padding after 0.4-0.8m above.Fermentation bed has been laid live pig to be caught up and has been raised.
Embodiment three
1. composite bacteria leaven preparation method
Slant strains cultivation → liquid first order seed (shaking flask) cultivation → liquid two stage (seeding tank) → fermentation tank culture → supercentrifuge → vacuum freeze drier → cracker → Counting alive microbial → bacterial classification combines → packs → finished product leavening in proportion
Seed culture: utilize any one in above-mentioned bacterial classification, conventional experiment condition is adopted to cultivate, namely with carbohydrate goods and materials for carbon source (said glucide can be in glucose, sucrose, lactose, fructose, maltose, starch one or more 1, with peptone, yeast extract or beef leaching thing for nitrogenous source, and add appropriate mineral salt and trace element, being deployed into solution as seed culture medium by deionized water, obtaining liquid spawn through cultivating, stand-by.Because the incubation of above bacterial classification is all with reference to cultural method described in Chinese bacterial classification catalogue, be all conventional laboratory facilities, therefore describe and conform to the principle of simplicity.
Slant strains is cultivated: each bacterial classification uses corresponding medium, inoculum concentration 6-12%, static gas wave refrigerator 24-48 hour under 30-35 DEG C of condition, puts into 4 DEG C of Refrigerator stores.
Wherein shake flask culture conditions: temperature 30-40 DEG C, rotating speed 100-200 beat/min, inoculum concentration 2-4%, time 20-35 hour.
Fermentation tank culture condition: temperature 30-35 DEG C, ventilation 10-13 square metre/h, tank pressure 0.05-0.10Mpa, inoculum concentration 3-10%, time 24-36 hour.
Bacterial classification combines in proportion: candida utili 15% qualified for mensuration bacterium number, the inferior Dbaly yeast 15% of the Chinese, geotrichum candidum 15%, Cellumomonas flavigena 12%, Lactobacillus plantarum 4%, Rhodopseudomonas palustris 4%, Trichoderma viride 10%, aspergillus niger 10%, bafillus natto 15% are carried out combination and make leavening.
Composite bacteria leaven has following proterties:
Pale powder, tasteless or slightly tart flavour, viable count reaches 2.2xl0
11individual/g, moisture is below 10%.
2. corn flour 70% is got in the activation of composite bacteria leaven, and wheat bran or thin chaff 30% mix, composite bacteria leaven inoculum concentration 5 ‰, amount of water 40-45%, mixes thoroughly three, under being placed on 2035 DEG C of conditions, cultivate 12-18 hour, centre turns 2 times, keeps product temperature 20-35 DEG C.Cultivation instrument can in basin, in plastic sack, heat and moisture preserving.When cultivation charge level turns white, mycelia is gathered, and has sour-sweet fragrance to apply.
3. the fermentation of organic bedding and padding
Organic bedding and padding are made up of the wood chip of 30% percent by volume, the rice husk of 30% percent by volume, the corn stalk powder of 20%.Composite bacteria leaven after activation is pressed 10kg/m
3the inoculation of organic bedding and padding, the 45-50% that adds water puddles evenly, heat and moisture preserving heap fermentation, and ferment summer 1-2 days, winter 3-5 days, organic bedding and padding central temperature reaches 35-40 DEG C, is fermentation and completes.
4. the making of ecologically raising pigs fermentation bed
Fermentation bed in colony house can selectively above formula and underground type according to subterranean water level, and the degree of depth will ensure between 0.8-l.Om, and bottom can lay loose ventilation bottom in advance, directly all lays the organic fermentation bedding and padding after 0.8-1.Om.Fermentation bed has been laid live pig to be caught up and has been raised.
Claims (5)
1. a preparation method for nuisanceless ecologically raising pigs fermentation bed, is characterized in that the composite bacteria leaven making ecologically raising pigs fermentation bed is made primarily of saccharomycete, lactic acid bacteria, actinomycetes, photosynthetic bacteria and filamentous fungi; Component in composite bacteria leaven forms by weight ratio: the inferior Dbaly yeast 10-15% of candida utili 10-20%, the Chinese, geotrichum candidum 10-15%, Cellumomonas flavigena 7-10%, Lactobacillus plantarum 2-5%, Rhodopseudomonas palustris 2-5%, Trichoderma viride 8-12%, aspergillus niger 6-10%, bafillus natto 12-17%.
2. the preparation method of nuisanceless ecologically raising pigs fermentation bed according to claim 1, it is characterized in that the preparation method of composite bacteria leaven is as follows: the saccharomycete cultivated by purifying, lactic acid bacteria, actinomycetes, photosynthetic bacteria and filamentous fungi mix in proportion, wherein candida utili 15-25%, the inferior Dbaly yeast 12-18% of the Chinese, geotrichum candidum 12-18%, Cellumomonas flavigena 8-12%, Lactobacillus plantarum 3-6%, Rhodopseudomonas palustris 3-6%, Trichoderma viride 8-10%, aspergillus niger 8-12%, bafillus natto 10-15%.
3. the preparation method of nuisanceless ecologically raising pigs fermentation bed according to claim 2, it is characterized in that saccharomycete, lactic acid bacteria, actinomycetes, the cultural method of photosynthetic bacteria and filamentous fungi is as follows: slant strains cultivation → liquid first order seed (shaking flask) cultivation → liquid two stage (seeding tank) → fermentation tank culture → supercentrifuge → vacuum freeze drier → cracker → Counting alive microbial → bacterial classification combines → pack → finished product leavening slant strains cultivation in proportion: each bacterial classification uses corresponding medium, inoculum concentration 6-12%, static gas wave refrigerator 36-48 hour under 30-35 DEG C of condition, put into 5 DEG C of Refrigerator stores,
Wherein shake flask culture conditions: temperature 30-30 DEG C, rotating speed 100-200 beat/min, inoculum concentration 2-4%, time 20-35 hour;
Fermentation tank culture condition: temperature 30-35 DEG C, ventilation 10-13 square metre/h, tank pressure 0.05-0.10Mpa, inoculum concentration 3-10%, time 24-36 hour.
4. realize the method for the preparation method of nuisanceless ecologically raising pigs fermentation bed described in claim 1, it is characterized in that composite bacteria leaven is as pad material fermentation agent organic in ecological colony house, preparation method is as follows: the activation of leavening: get corn flour 35-80%, thin chaff 35-80% mixes, leavening inoculum concentration 3-8 ‰, amount of water 50-60%, three is mixed thoroughly, under being placed on 25-40 DEG C of condition, cultivate 15-20 hour, centre turns 2 times, maintenance product temperature 25-40 DEG C, cultivation instrument can in basin, in plastic sack, heat and moisture preserving, when cultivation charge level turns white, mycelia is gathered, sour-sweet fragrance is had to apply:
Organic pad material fermentation: organic bedding and padding can be one or more the mixtures in sawdust, rice husk, straw powder etc., presses 8-l2kg/m by the leavening after activation
3the inoculation of organic bedding and padding, the 35-40% that adds water puddles evenly, heat and moisture preserving heap fermentation, and ferment summer 2-3 days, winter 3-5 days, organic bedding and padding central temperature reaches 30-35 DEG C, is fermentation and completes.
5. the preparation method of nuisanceless ecologically raising pigs fermentation bed described in claim 1, is characterized in that composite bacteria leaven is raised pigs for ecological method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410414866.7A CN105340764A (en) | 2014-08-22 | 2014-08-22 | Manufacturing method for pollution-free ecological pig-raising fermentation bed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410414866.7A CN105340764A (en) | 2014-08-22 | 2014-08-22 | Manufacturing method for pollution-free ecological pig-raising fermentation bed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105340764A true CN105340764A (en) | 2016-02-24 |
Family
ID=55317054
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410414866.7A Withdrawn CN105340764A (en) | 2014-08-22 | 2014-08-22 | Manufacturing method for pollution-free ecological pig-raising fermentation bed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105340764A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105907667A (en) * | 2016-04-22 | 2016-08-31 | 广西智宝科技有限公司 | Enhanced fermentation bed composite strain |
| CN106578522A (en) * | 2016-12-13 | 2017-04-26 | 河南广安生物科技股份有限公司 | Preparation method of mixed-culture solid state fermentation piglet compound feed |
| CN107079680A (en) * | 2017-03-29 | 2017-08-22 | 王耿文 | A kind of implantation methods of selenium-rich potato |
| CN107114085A (en) * | 2017-03-29 | 2017-09-01 | 王耿文 | A kind of implantation methods of selenium-enriched sweet potato |
| CN109526536A (en) * | 2017-07-31 | 2019-03-29 | 张家港爱尔乐信息科技有限公司 | A kind of stalk fermentation bed Inoculant and preparation method thereof and application method |
-
2014
- 2014-08-22 CN CN201410414866.7A patent/CN105340764A/en not_active Withdrawn
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105907667A (en) * | 2016-04-22 | 2016-08-31 | 广西智宝科技有限公司 | Enhanced fermentation bed composite strain |
| CN106578522A (en) * | 2016-12-13 | 2017-04-26 | 河南广安生物科技股份有限公司 | Preparation method of mixed-culture solid state fermentation piglet compound feed |
| CN107079680A (en) * | 2017-03-29 | 2017-08-22 | 王耿文 | A kind of implantation methods of selenium-rich potato |
| CN107114085A (en) * | 2017-03-29 | 2017-09-01 | 王耿文 | A kind of implantation methods of selenium-enriched sweet potato |
| CN109526536A (en) * | 2017-07-31 | 2019-03-29 | 张家港爱尔乐信息科技有限公司 | A kind of stalk fermentation bed Inoculant and preparation method thereof and application method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101565684A (en) | Method for making fermenting bed for ecological pig breeding by using composite strain culture | |
| CN106173363B (en) | The method for producing cattle and sheep complete feed as raw material staged fermentation using edible fungus bran | |
| CN105176881B (en) | A method of producing the engineering microbial inoculum and its production active bio-organic fertilizer of active bio-organic fertilizer | |
| CN106386163A (en) | Method for cultivating edible fungi by using crop straw | |
| CN102491802A (en) | Tea seed cake biological organic fertilizer and production method thereof | |
| CN101775359A (en) | Special microorganism composite bacterial agent for directly decomposing and fermenting crops straws to generate marsh gas and application method thereof | |
| CN102924156A (en) | Culture medium and cultural method for cultivating coprinus comatus | |
| CN105255767B (en) | A kind of complex microorganism preparations and fowl fermenting bed padding | |
| CN107646616A (en) | A kind of preparation method of floriculture substrate | |
| CN103380732A (en) | Preparation method for zymophyte being added in pig fermentation bed | |
| CN111789004B (en) | Method for realizing ecological cycle by combining sugarcane field planting and breeding | |
| CN105340764A (en) | Manufacturing method for pollution-free ecological pig-raising fermentation bed | |
| CN107912267A (en) | A kind of preparation method of Chinese fir seedling medium | |
| CN108516905A (en) | A method of preparing organic fertilizer using agriculture wastes | |
| CN104719170A (en) | Pig house and method for resource utilization of straw as fermentation bed padding for pigs | |
| CN104987156A (en) | Lyophyllum fumosurn culture medium using fermented bran and method for cultivating lyophyllum fumosurn | |
| CN105330407A (en) | Cultivation medium capable of increasing yield of big clitocybe and preparation method thereof | |
| CN107903112A (en) | A kind of preparation method of eucalyptus seedling culture substrate | |
| CN106234755B (en) | The method for producing cattle and sheep complete feed as raw material staged fermentation using bagasse | |
| CN103524161A (en) | Process for fermenting organic fertilizer by poultry and livestock excrement | |
| CN101885640A (en) | Method for preparing mushroom medium by using microbial composite bacteria | |
| CN102138637A (en) | Formula and preparation process of transforming agent of organic feed of grain-free biomass | |
| CN109180376A (en) | A kind of biomass fertilizer and preparation method thereof for discarding padding production with fermentation bed using pig-breeding | |
| CN1653936B (en) | Method for preparing composite micro-ecological enzyme for feeding the ruminant by using disused mushroom dregs | |
| CN109105271A (en) | A kind of padding and preparation method thereof of vaccary cowshed |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C04 | Withdrawal of patent application after publication (patent law 2001) | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20160224 |