CN101810155A - Tetranychina harti feeder - Google Patents

Abstract

The invention relates to a feeder for wood sorrel such as spider mites, which belongs to the technical field of entomological experiments. The feeder consists of an upper feeding room and a lower pool. The detached leaves of safflower sorrel with stalks are upright in the feeder, the blade part where the mites are located is suspended in the breeding room, and the petiole enters below the water surface in the pool through the stalk tube at the bottom of the breeding room. When the present invention is used to raise wood sorrel such as spider mites, the leaves of safflower sorrel leaves are far away from the fresh-keeping liquid, are not easy to turn yellow and moldy, the leaves are dry, and mites can move freely on the leaves, and their living conditions are more natural . The breeding room is made of disposable plastic water cups and gauze, which is closed, air-permeable and light-transmitting, which can prevent mites from escaping and disappearing, and make the microenvironment of mites more consistent with the set breeding conditions. The parts of the feeder can be easily disassembled and assembled, which makes the microscope observation and replacement of the leaves more convenient.

Description

Sorrel like spider mite feeder

technical field

The invention relates to a device for raising wood sorrel such as spider mites, and belongs to the technical field of insect (acari) science experiments.

Background technique

The sorrel-like spider mite is an understudied species of spider mite. In addition to feeding on the wild wood sorrel that can be used as traditional Chinese medicine, the mite also seriously harms the safflower wood sorrel used for landscaping.

In the study of spider mites, it is often necessary to carry out individual feeding or quantitative feeding of the mite under set conditions, and to observe regularly during the feeding process. For the spider mites that mainly feed on the leaves of the host, they can be raised on leaves picked from the host plant (in vitro leaf method), or on leaves of living plants (in vivo leaf method). In the next case, certain methods or utensils should be adopted to keep the leaves fresh and prevent mites from escaping. In the latter case, escape prevention is also required. In order to ensure the accuracy of the research results, the breeding methods or utensils used should be able to effectively control the abnormal death and disappearance of spider mites, and try to ensure that the leaves are kept fresh, the living conditions of the mites are natural, the microenvironment where the mites live and the equipment The specified conditions (temperature, humidity, etc.) should be consistent, and it should be convenient to observe and operate. But at present in existing spider mite raising method or utensil, also do not have which one can meet above-mentioned requirement simultaneously and adapt to the characteristics of wood sorrel such as spider mite (individual is bigger, foot is longer, and host plant blade is little).

The representative method or utensils that have been reported to raise spider mites as food with detached leaves include water tray method, small chamber method, Hershey's feeder, Tiancheng's feeder, etc. What they have in common is: the detached leaves (or leaves) plate) on the wet base (water surface or wet absorbent materials such as absorbent paper, cotton, sponge, etc.) to keep fresh, and prevent spider mites from escaping with the water surface around the leaves, wet absorbent paper strips, and cell walls. Therefore, it can be collectively referred to as the in vitro wet-base leaf method. This method has the advantage of being more convenient to observe mites, but it also has many defects: (1) The surface humidity of the leaves is too high because they are close to the wet base, which makes the feet of spider mites easy to stick to dirt such as feces and hinders their activities, and makes the leaves It is easy to turn yellow and moldy and affect its lifespan and nutrition; (2) spider mites can only move on the upward leaf surface, and their positions are often unnatural; (3) due to the evaporation of moisture from the wet base, the leaf surface temperature is significantly lower than the ambient temperature (ie set temperature); (4) when using the water tray method, many spider mites will stray into the wet base material around the leaves and cause death or disappearance, especially species with long body thighs (such as wood sorrel such as spider mite ). These flaws affect the accuracy of test data, and even make the test fail completely. Individual feeding or quantitative feeding of spider mites on living plants is usually carried out by bagging (covering the branches where the mites are located with plastic bags) or cage method (clamping special small cages on the leaves). The main advantage of this type of method is that there is generally no need to consider the problem of leaf preservation during the breeding process. The main disadvantage is that it is very inconvenient to carry out regular microscopic examination of the mites during the breeding process, and it is not suitable for wood sorrel (because the wood sorrel plant is very weak. , and the leaves are small).

Contents of the invention

Technical problem The object of the present invention is to provide a kind of wood sorrel such as spider mite feeder, and this feeder can meet the following requirements simultaneously: Can effectively control the abnormal death rate and disappearance of wood sorrel such as spider mite in the raising process; Leaf preservation The time is long; the living state of mites is more natural; the microenvironment where mites live is basically the same as the set conditions; the observation of mites is more convenient. Adopting the feeder can greatly improve the accuracy of research data on wood sorrel such as spider mites.

Technical solution In order to achieve the above-mentioned purpose and requirement, the technical solution adopted in the present invention is:

A feeder for wood sorrel such as spider mites is composed of an upper feeding room and a lower pool. The feeding room is framed on the pool. The breeding room is formed by interlocking a bottom cup and a cover. The bottom cup is a disposable plastic water cup with a height of about 60 mm. There is a hole in the center of the bottom surface for inserting and fixing the insertion handle tube; the cover consists of a cover ring, The cover tube clamps the gauze cover, wherein the cover ring is a 20mm high part cut from the upper part of a disposable plastic water cup, the cover tube is most of the upper part of another plastic water cup, and the side length of the gauze cover is 40mm larger than the diameter of the upper mouth of the water cup Left and right square gauze pieces. The insertion tube is a 35 mm long section of a plastic straw with a diameter of 5 mm, and the top end is wrapped with gauze. The pool is a glass beaker or other transparent thick-walled cylindrical container, and its caliber is smaller than the upper caliber of the disposable plastic water cup but larger than the bottom diameter of the plastic cup.

The method of using the feeder is as follows: remove the cover of the breeding room, insert the petiole end of the safflower sorrel leaf through the gap between the gauze fibers at the upper end of the stalk tube, and enter the water surface of the pool after passing through the stalk tube, and the leaf part hangs upright. Put it in the breeding room, pick mites on the leaves according to needs, and then reset the cover; when you need to observe the mites on the leaves during the feeding process, you can take out the leaves after removing the cover, insert the leaves back after inspection, and then replace the cover reset.

Beneficial effect

The abnormal mortality rate of wood sorrel such as spider mites is lower than that of various in vitro wet base leaf methods; the missing rate of mites is much lower than that of water tray method; The basal leaf method is much smaller, and smaller than the cage method; the living state of the mites is more natural than that of most existing methods; the observation of the mites is more convenient than the live leaf method; the preservation time of the leaves is longer than that of the isolated wet basal leaf method. More; the replacement of leaves is more convenient than various in vitro wet-base leaf methods; at the same time, the feeder is easy to obtain materials, easy to make, and convenient to clean and store.

When using the present invention to raise wood sorrel such as spider mites, the leaves of safflower sorrel are upright in the feeder, and the blade part is naturally upright suspended in the breeding room, away from the fresh-keeping liquid, and it is not easy to turn yellow and moldy. Dry and dry, mites can move freely on the leaves, and their living conditions are more natural. The petiole enters the pool through the stalk pipe, absorbs water through the broken end, and plays a good role in keeping the leaves fresh. The breeding room is basically closed (closely fastened by the cover and the bottom cup), which can prevent mites from falling into the water and dying or escaping and disappearing. The materials used are disposable plastic water cups and medical gauze, etc. The light transmittance makes the microenvironment where the mites live are more consistent with the set conditions. The pool is acted as a glass beaker or other transparent thick-walled cylindrical container, which not only has light transmission, but also makes the center of gravity of the feeder more stable. The parts of the feeder can be easily disassembled and assembled, which makes the microscope observation and replacement of the leaves more convenient.

Description of drawings

Accompanying drawing shows the structure of an example of above-mentioned sorrel such as spider mite feeder.

Figure 1 is the overall rendering of the feeder.

Fig. 2 is a front view of some parts of the breeding room.

Figure 3 is the cover of the breeding room.

Fig. 4 is the bottom cup of the feeder equipped with a stalk tube.

In the figure: 1. The cover ring of the feeding room; 2. The gauze cover of the feeding room; 3. The cover tube of the feeding room; 4. The leaves of Sorrel safflower; 5. The bottom cup of the feeding room; 7. Water surface; 8. Beaker (pool); 9. Petiole of safflower sorrel.

Detailed ways

The making and using method of example shown in accompanying drawing are as follows:

Production of the main parts of the breeding room: Take a disposable plastic water cup (bottom diameter 45mm, upper inner diameter 66.5mm, height 100mm), cut the upper part 20mm and the lower part 60mm high respectively as the cover ring (1) and the bottom of the breeding room Cup (5); Get 1 plastic cup in addition, remove the bottom, leave most (high 90mm) as the cover cylinder (3) of feeding room; Cut out the square gauze piece that side length is 105mm as the feeding room from the large piece of gauze Gauze cover (2); cut a 35mm long section from the drinking straw with a diameter of 5mm, wrap a circle of double-sided adhesive tape at one end, wrap gauze on the outside, and cut the other end into an inclined plane to form a handle pipe (6); Drill a round hole with a diameter slightly larger than 5mm in the bottom center of the bottom cup (5) of the feeding room, and then insert the tip of the stalk tube downward into the hole and fix it to make a bottom cup of the feeding room with a stalk tube (as shown in the figure). 4); Cover the gauze cover (2) on the upper mouth of the cover tube, then press down with the cover ring, clamp the gauze, and make the cover of the breeding room (as shown in Figure 3).

Assembly of the feeder: Take a 100ml glass beaker (8), pour about 60ml of water or nutrient solution into it; place the lower part of the bottom cup lightly in the mouth of the beaker, and insert the broken end of the safflower sorrel leaf petiole (9) through the stalk Insert the gauze fiber gap at the upper end of the pipe (6) below the water surface (7); slightly lift the bottom cup, insert the lower end of the cover into the mouth of the bottom cup, make the overlapping parts close to each other, and put it down gently.

The use of the feeder: take off the cover of the feeding room, you can connect mites on the blade (4) as required, and then reset the cover. When it is necessary to observe the mites on the leaves during the breeding process, the leaves can be taken out after the cover is removed. After the inspection, the leaves are inserted back and the cover is reset.

Claims (5)

1. Tetranychina harti feeder, it is characterized in that: feeder is made up of the receptacle on top and the pond of bottom, and the receptacle frame is on the pond, and both were both relatively independent, communicated with the inserting handle pipe that is fixed in the receptacle bottom again.

2. according to the described receptacle of claim 1, it is characterized in that: receptacle is fastened mutually by end cup and cover and forms, and end cup is the high part of the about 60mm in a kind of disposable plastic water tumbler bottom, and its bottom center is equipped with a hole, is used for inserting and fixing inserting handle pipe; Cover is formed by decorative cover, cover collet chuck tight feeder cloth lid, wherein decorative cover is the high part of 20mm from the intercepting of disposable plastic water tumbler top, the cover tube is the major part on another plastics water tumbler top, and the gauze lid is the square gauze piece about the big 40mm of side ratio water tumbler back cut diameter.

3. according to claim 1 or 2 described receptacles, it is characterized in that: long one section of 35mm in the plastic suction pipe that described inserting handle pipe is diameter 5mm, the top is wrapped up in gauze.

4. according to the described feeder of claim 1, it is characterized in that: described pond is glass beaker or other transparent heavy wall hydrostatic columns, and its bore is less than bore on the disposable plastic water tumbler but greater than footpath at the bottom of the plastic cup.

5. the using method of the described feeder of one of claim 1-4, it is characterized in that: the cover that takes off receptacle, the petiole broken ends of fractured bone of window-box oxalis leaf is inserted via inserting handle pipe upper end gauze fibre gap, by the laggard underwater of going into the pond of inserting handle pipe, blade-section uprightly is suspended in the receptacle, can on blade, connect mite as required, then cover be resetted; When needing to observe the mite on the blade in the feeding process, can take out leaf after taking off cover, check and leaf is returned after finishing slottingly, again cover be resetted.

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